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relationships of plant pathogenic enterobacteria based on partial atpd, cara, and reca as individual and concatenated nucleotide and peptide sequences.relationships of the genera in the enterobacteriaceae containing plant pathogenic species: brenneria, dickeya, enterobacter, erwinia, pantoea, pectobacterium, and samsonia, were investigated by comparison of their nucleotide and peptide sequences of atpd, cara, reca, and the concatenated sequences. erwinia spp. and pantoea spp., with pectobacterium cypripedii, formed a group distinct from other pathogenic taxa. pectobacterium, brenneria, dickeya, and samsonia formed a contiguous clade. samsonia ...200717451899
bacterial ghost technology for pesticide delivery.bacterial ghosts are nondenaturated empty cell envelopes of gram-negative bacteria produced by e-mediated lysis. such envelopes from the plant-adhering bacterium pectobacterium cypripedii were tested for their ability to adhere to plant material and to be used as carriers for pesticide delivery. we show, using fluorescence-labeled p. cypripedii ghosts, that depending on the target plants 55 or 10% (rice or soya, respectively) of the applied bacterial ghosts was retained on the leaves after heavy ...200415373403
pantoea rodasii sp. nov., pantoea rwandensis sp. nov. and pantoea wallisii sp. nov., three novel pantoea species isolated from eucalyptus.several gram-negative, facultatively anaerobic bacterial isolates were obtained from eucalyptus seedlings showing symptoms of bacterial blight and dieback in colombia, rwanda and south africa. partial 16s rrna gene sequencing, together with partial gyrb sequencing, placed the isolates in the genus pantoea and indicated they constituted three novel species. multilocus sequence analysis (mlsa) based on partial sequences of gyrb, rpob, infb and atpd revealed p. dispersa, p. eucrina and p. cypripedi ...201121841003
a temperature-regulated campylobacter jejuni gluconate dehydrogenase is involved in respiration-dependent energy conservation and chicken colonization.campylobacter jejuni is a gastrointestinal pathogen of humans but can asymptomatically colonize the avian gut. c. jejuni therefore grows at both 37 degrees c and 42 degrees c, the internal temperatures of humans and birds respectively. microarray and proteomic studies on temperature regulation in c. jejuni strain 81-176 revealed the upregulation at 42 degrees c of two proteins, cj0414 and cj0415, orthologous to gluconate dehydrogenase (gadh) from pectobacterium cypripedii. 81-176 demonstrated ga ...200818284594
emended description of the genus pantoea, description of four species from human clinical samples, pantoea septica sp. nov., pantoea eucrina sp. nov., pantoea brenneri sp. nov. and pantoea conspicua sp. nov., and transfer of pectobacterium cypripedii (hori 1911) brenner et al. 1973 emend. hauben et al. 1998 to the genus as pantoea cypripedii comb. nov.bacterial strains belonging to dna hybridization groups (hg) ii, iv and v, in the erwinia herbicola-enterobacter agglomerans complex, of brenner et al. [int j syst bacteriol 34 (1984), 45-55] were suggested previously to belong to the genus pantoea, but have never been formally described and classified. additionally, it has been shown in several studies that pectobacterium cypripedii is more closely related to species of pantoea than to those of pectobacterium. in this study, the phylogenetic po ...201019946052
phosphate solubilizing rhizobacteria from an organic farm and their influence on the growth and yield of maize (zea mays l.).organic farming is gaining popularity all over the world as it avoids the use of synthetic chemicals. plant production in organic farming mainly depends on nutrient release as a function of mineralization processes in soils. in the present study, efficient phosphate mineralizing bacteria were isolated and their efficacy tested in plant mineral uptake and soil fertility of an organic field. amongst 12 p-solubilizing bacteria (psb) isolated from an organic field, two isolates were selected for fie ...201324005179
cloning, sequencing, and heterologous expression of an erwinia cypripedii 314b lactonase specific for l-alpha-hydroxyglutaric acid gamma-lactone.the gene for a lactonase that stereospecifically hydrolyzes (s)-5-oxo-2-tetrahydrofurancarboxylic acid to l-alpha-hydroxyglutaric acid was isolated from erwinia cypripedii 314b. determination of the nucleotide sequence showed that the gene consists of a single open reading frame of 1,152 bp that encodes a 383-amino-acid protein. comparison of the sequence of the predicted protein to that of the enzyme purified from e. cypripedii 314b revealed an n-terminal signal sequence of 19 amino acids. the ...200616328442
purification and characterization of a lactonase from erwinia cypripedii 314b that hydrolyzes ( s)-5-oxo-2-tetrahydrofurancarboxylic acid.a bacterium, strain 314b, able to assimilate ( s)-5-oxo-2-tetrahydrofurancarboxylic acid was isolated from soil and identified as erwinia cypripedii. a lactonase hydrolyzing ( s)-5-oxo-2-tetrahydrofurancarboxylic acid to l-alpha-hydroxyglutaric acid was purified 63-fold with 2% recovery from crude extracts of this bacterium to homogeneity as judged by sds-page. the molecular masses estimated by sds-page and gel filtration were 41 kda and 79 kda, respectively. the maximum activity was observed at ...200314576976
genotyping of bacteria belonging to the former erwinia genus by pcr-rflp analysis of a reca gene fragment.genotypic characterization, based on the analysis of restriction fragment length polymorphism of the reca gene fragment pcr product (reca pcr-rflp), was performed on members of the former erwinia genus. pcr primers deduced from published reca gene sequences of erwinia carotovora allowed the amplification of an approximately 730 bp dna fragment from each of the 19 erwinia species tested. amplified reca fragments were compared using rflp analysis with four endonucleases (alui, hinfi, tasi and tru1 ...200211832521
transfer of pectobacterium chrysanthemi (burkholder et al. 1953) brenner et al. 1973 and brenneria paradisiaca to the genus dickeya gen. nov. as dickeya chrysanthemi comb. nov. and dickeya paradisiaca comb. nov. and delineation of four novel species, dickeya dadantii sp. nov., dickeya dianthicola sp. nov., dickeya dieffenbachiae sp. nov. and dickeya zeae sp. nov.a collection of 75 strains of pectobacterium chrysanthemi (including all biovars and pathovars) and the type strains of brenneria paradisiaca (cfbp 4178(t)) and pectobacterium cypripedii (cfbp 3613(t)) were studied by dna-dna hybridization, numerical taxonomy of 121 phenotypic characteristics, serology and 16s rrna gene-based phylogenetic analyses. from analysis of 16s rrna gene sequences, it was deduced that p. chrysanthemi strains and b. paradisiaca cfbp 4178(t) formed a clade distinct from th ...200516014461
phylogeny and identification of pantoea species associated with plants, humans and the natural environment based on multilocus sequence analysis (mlsa).species belonging to the genus of pantoea are commonly isolated from plants, humans and the natural environment. the species of the genus are phenotypically closely related, making rapid identification of pantoea strains to the species level difficult. multilocus sequence analysis (mlsa) was evaluated as a means for rapid classification and identification of pantoea strains. four housekeeping genes, gyrb, rpob, atpd and infb, were sequenced for strains assigned to the genus. included in the stud ...200819008066
extracellular oxidation of d-glucose by some members of the enterobacteriaceae.extracellular d-glucose oxidation by 5 enterobacterial species was studied with the purpose of selecting conditions useful for taxonomic studies. extracellular production of gluconate from 14c-glucose by bacterial cells was evidenced by deae-cellulose paper chromatography. escherichia coli oxidized glucose only when pyrroloquinoline quinone (pqq) was added, whereas serratia marcescens, yersinia frederiksenii, erwinia cypripedii and cedecea lapagei oxidized d-glucose without added pqq. 2-deoxyglu ...19883382545
phylogenetic analysis of erwinia species based on 16s rrna gene sequences.the phylogenetic relationships of the type strains of 16 erwinia species were investigated by performing a comparative analysis of the sequences of the 16s rrna genes of these organisms. the sequence data were analyzed by the neighbor-joining method, and each branch was supported by moderate bootstrap values. the phylogenetic tree and sequence analyses confirmed that the genus erwinia is composed of species that exhibit considerable heterogeneity and form four clades that are intermixed with mem ...19979336906
cloning and expression of a gene cluster encoding three subunits of membrane-bound gluconate dehydrogenase from erwinia cypripedii atcc 29267 in escherichia coli.we have cloned the gene cluster encoding three subunits of membrane-bound gluconate dehydrogenase (gadh) from erwinia cypripedii atcc 29267 in escherichia coli by performing a direct-expression assay. the positive clone converted d-gluconate to 2-keto-d-gluconate (2kdg) in the culture medium. nucleotide sequence analysis of the gadh clone revealed that the cloned fragment contained the complete structural genes for a 68-kda dehydrogenase subunit, a 47-kda cytochrome c subunit, and a 24-kda subun ...19979352901
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