| proposal for two new genera, brevibacillus gen. nov. and aneurinibacillus gen. nov. | 16s rrna gene sequences of the type strains of 11 species belonging to the bacillus brevis and bacillus aneurinolyticus groups were determined. on the basis of the results of gene sequence analyses, these species were separated into two clusters. the b. brevis cluster included 10 species, namely, bacillus brevis, bacillus agri, bacillus centrosporus, bacillus choshinensis, bacillus parabrevis, bacillus reuszeri, bacillus formosus, bacillus borstelensis, bacillus laterosporus, and bacillus thermo ... | 1996 | 8863420 |
| s-layered aneurinibacillus and bacillus spp. are susceptible to the lytic action of pseudomonas aeruginosa membrane vesicles. | when s-layered strains of bacillus stearothermophilus and aneurinibacillus thermoaerophilus, possessing s-layers of different lattice type and lattice constant as well as s-(glyco)protein chemistry, and isogenic s-layerless variants were subjected to membrane vesicles (mvs) from p. aeruginosa during plaque assays on plates or cfu measurements on cell suspensions, all bacterial types lysed. electron microscopy of negative stains, thin sections, and immunogold-labelled mv preparations revealed tha ... | 1998 | 9573179 |
| microbial diversity in hot synthetic compost as revealed by pcr-amplified rrna sequences from cultivated isolates and extracted dna. | high-temperature (>/=60 degrees c) synthetic food waste compost was examined by cultivation-dependent and -independent methods to determine predominant microbial populations. fluorescent direct counts totaled 6.4 (+/-2.5)x10(10) cells gdw(-1) in a freeze-dried 74 degrees c compost sample, while plate counts for thermophilic heterotrophic aerobes averaged 2.6 (+/-1.0)x10(8) cfu gdw(-1). a pre-lysis cell fractionation method was developed to obtain community dna and a suite of 16s and 18s rdna-tar ... | 2001 | 11295460 |
| paenibacillus jamilae sp. nov., an exopolysaccharide-producing bacterium able to grow in olive-mill wastewater. | endospore-forming strains were isolated from corn-compost treated with olive-mill wastewater ('alpechin'). the strains were taxonomically studied and proposed as a novel paenibacillus species. these organisms (strains b.3t, b.7 and b.9) were particularly distinguishable from other aerobic spore-forming species by their ability to grow optimally in 100% (v/v) olive-mill wastewater at 30 degrees c and ph 7.0 and concomitant production of an interesting exopolysaccharide. chemotaxonomic analysis re ... | 2001 | 11594596 |
| osmotically regulated synthesis of the compatible solute ectoine in bacillus pasteurii and related bacillus spp. | by using natural-abundance (13)c-nuclear magnetic resonance spectroscopy and high-performance liquid chromatography (hplc) analysis we have investigated the types of compatible solutes that are synthesized de novo in a variety of bacillus species under high-osmolality growth conditions. five different patterns of compatible solute production were found among the 13 bacillus species we studied. bacillus subtilis, b. licheniformis, and b. megaterium produced proline; b. cereus, b. circulans, b. th ... | 2002 | 11823218 |
| homologs of the rml enzymes from salmonella enterica are responsible for dtdp-beta-l-rhamnose biosynthesis in the gram-positive thermophile aneurinibacillus thermoaerophilus dsm 10155. | the glycan chains of the surface layer (s-layer) glycoprotein from the gram-positive, thermophilic bacterium aneurinibacillus (formerly bacillus) thermoaerophilus strain dsm 10155 are composed of l-rhamnose- and d-glycero-d-manno-heptose-containing disaccharide repeating units which are linked to the s-layer polypeptide via core structures that have variable lengths and novel o-glycosidic linkages. in this work we investigated the enzymes involved in the biosynthesis of thymidine diphospho-l-rha ... | 2002 | 12147463 |
| an aneurinibacillus sp. strain am-1 produces a proline-specific aminopeptidase useful for collagen degradation. | we have been for a species of thermophilic bacteria that can effectively decompose collagen and collagen peptides that tend to be hard-to-degrade proteins because of their high content of proline residues. this study focused upon the enzymatic degradation of prolyl peptides by thermophilic bacteria. | 2004 | 15012820 |
| overexpression, purification, crystallization and preliminary x-ray crystallographic studies of a proline-specific aminopeptidase from aneurinibacillus sp. strain am-1. | to elucidate the structure and molecular mechanism of a characteristic proline-specific aminopeptidase produced by the thermophile aneurinibacillus sp. strain am-1, its gene was cloned and the recombinant protein was overexpressed in escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. x-ray diffraction data were collected to 1.8 a resolution from the recombinant aminopeptidase crystal. the crystals belong to the orthorhombic space group p2(1)2(1)2, with un ... | 2006 | 17142913 |
| the x-ray structure of dtdp-4-keto-6-deoxy-d-glucose-3,4-ketoisomerase. | the repeating unit of the glycan chain in the s-layer of the bacterium aneurinibacillus thermoaerophilus l420-91(t) is composed of four alpha-d-rhamnose molecules and two 3-acetamido-3,6-dideoxy-alpha-d-galactose moieties (abbreviated as fucp3nac). formation of the glycan layer requires nucleotide-activated sugars as the donor molecules. whereas the enzymes involved in the synthesis of gdp-rhamnose have been well characterized, less is known regarding the structures and enzymatic mechanisms of t ... | 2007 | 17459872 |
| elucidating the formation of 6-deoxyheptose: biochemical characterization of the gdp-d-glycero-d-manno-heptose c6 dehydratase, dmha, and its associated c4 reductase, dmhb. | 6-deoxyheptose is found within the surface polysaccharides of several bacterial pathogens. in yersinia pseudotuberculosis, it is important for the barrier function of the o-antigen in vitro and for bacterial dissemination in vivo. the putative c6 dehydratase dmha and c4 reductase dmhb, that were identified as responsible for 6-deoxyheptose synthesis based on genetics data, represent potential therapeutical targets. their detailed biochemical characterization is presented herein. the substrate, g ... | 2009 | 19610666 |
| evaluation of the vitek2 bcl card for identification of bacillus species and other aerobic endosporeformers. | to evaluate the performance of the vitek2 bacillus identification card (bcl) for the identification of aerobic endospore-forming bacteria, using fresh isolates and reference strains. | 2010 | 19943885 |
| tdp-mycaminose biosynthetic pathway revised and conversion of desosamine pathway to mycaminose pathway with one gene. | analysis of the tylosin gene cluster in streptomyces fradiae uncovered an orf, tyl1a, homologous to a hexose 3,4-isomerase found in aneurinibacillus thermoaerophilus. inclusion of the tyl1a gene along with other mycaminose biosynthetic genes (tylb, tylm1, tylm2, tylm3) identified in previous studies in an in vivo expression system successfully reconstituted the mycaminose pathway. expression of tyl1a alone in the s venezuelae kdesi mutant converted a desosamine pathway to a mycaminose pathway. t ... | 2005 | 16131199 |
| brevibacillus levickii sp. nov. and aneurinibacillus terranovensis sp. nov., two novel thermoacidophiles isolated from geothermal soils of northern victoria land, antarctica. | thirteen strains of endospore-forming bacteria were isolated from geothermal soils at cryptogam ridge, the north-west slope of mt melbourne, and at the vents and summit of mt rittmann in northern victoria land, antarctica. 16s rrna gene sequencing, sds-page and routine phenotypic characterization tests indicated that the seven isolates from the north-west slope of mt melbourne represent a novel species of brevibacillus and that the six isolates from cryptogam ridge and the vents and summit of mt ... | 2005 | 15879231 |
| reclassification of brevibacillus brevis strains ncimb 13288 and dsm 6472 (=nrrl nrs-887) as aneurinibacillus danicus sp. nov. and brevibacillus limnophilus sp. nov. | comparison of the hypervariable region (269-279 bases in length) at the 5' end of the 16s rdna sequences of 29 bacterial strains that were identified previously as brevibacillus brevis showed that 13 strains clustered with aneurinibacillus species, eight strains clustered with bacillus species and eight strains clustered with brevibacillus species. based on dna-dna hybridization results, 27 strains, not including [brevibacillus brevis] ncimb 13288 and [brevibacillus brevis] dsm 6472, were reiden ... | 2004 | 15023954 |
| a convenient synthesis of gdp d-glycero-alpha-d-manno-heptopyranose. | gdp d-glycero-alpha-d-manno-heptopyranose has been prepared in good overall yield from 2,3,4,6,7-penta-o-acetyl-d-glycero-d-manno-heptopyranose by a short-step synthesis. phosphitylation using the phosphoramidite procedure afforded the alpha-anomer in high selectivity. subsequent oxidation and partial deprotection gave the acetylated phosphate derivative, which was subjected to the coupling reaction with gmp-morpholidate to furnish the acetylated heptose nucleoside diphosphate in good yield. de- ... | 2004 | 14659681 |
| biosynthesis of dtdp-3-acetamido-3,6-dideoxy-alpha-d-galactose in aneurinibacillus thermoaerophilus l420-91t. | the glycan chain of the s-layer protein of aneurinibacillus thermoaerophilus l420-91t (dsm 10154) consists of d-rhamnose and 3-acetamido-3,6-dideoxy-d-galactose (d-fucp3nac). thymidine diphosphate-activated d-fucp3nac serves as precursor for the assembly of structural polysaccharides in gram-positive and gram-negative organisms. the biosynthesis of dtdp-3-acetamido-3,6-dideoxy-alpha-d-galactose (dtdp-d-fucp3nac) involves five enzymes. the first two steps of the reaction are catalyzed by enzymes ... | 2003 | 12740380 |
| the first biantennary bacterial secondary cell wall polymer and its influence on s-layer glycoprotein assembly. | the cell surface of aneurinibacillus thermoaerophilus dsm 10155 is covered with a square surface (s)-layer glycoprotein lattice. this s-layer glycoprotein, which was extracted with aqueous buffers after a freeze-thaw cycle of the bacterial cells, is the only completely water-soluble s-layer glycoprotein to be reported to date. the purified s-layer glycoprotein preparation had an overall carbohydrate content of 19%. detailed chemical investigations indicated that the s-layer o-glycans of previous ... | 2002 | 12201818 |
| heterotrophic bacteria growing in association with methylococcus capsulatus (bath) in a single cell protein production process. | the methanotrophic bacterium methylococcus capsulatus (bath) grows on pure methane. however, in a single cell protein production process using natural gas as methane source, a bacterial consortium is necessary to support growth over longer periods in continuous cultures. in different bioreactors of norferm danmark a/s, three bacteria consistently invaded m. capsulatus cultures growing under semi-sterile conditions in continuous culture. these bacteria have now been identified as a not yet descri ... | 2002 | 12073128 |
| simplified technique for identification of the aerobic spore-forming bacteria by phenotype. | the use of modern research approaches of genetics, biochemistry and molecular biology has led to progress in bacterial taxonomy. systematic study of the aerobic spore-forming bacteria has resulted in the realignment of the genus bacillus into several new genera. in the meantime, the identification process has become more difficult for the non-specialist in bacillus taxonomy. this paper presents a key for the simplified phenotypic identification of the mesophilic, aerobic, spore-forming bacteria ... | 2001 | 11491334 |
| biosynthesis of nucleotide-activated d-glycero-d-manno-heptose. | the glycan chain repeats of the s-layer glycoprotein of aneurinibacillus thermoaerophilus dsm 10155 contain d-glycero-d-manno-heptose, which has also been described as constituent of lipopolysaccharide cores of gram-negative bacteria. the four genes required for biosynthesis of the nucleotide-activated form gdp-d-glycero-d-manno-heptose were cloned, sequenced, and overexpressed in escherichia coli, and the corresponding enzymes gmha, gmhb, gmhc, and gmhd were purified to homogeneity. the isomera ... | 2001 | 11279237 |
| identification of two gdp-6-deoxy-d-lyxo-4-hexulose reductases synthesizing gdp-d-rhamnose in aneurinibacillus thermoaerophilus l420-91t. | the glycan repeats of the surface layer glycoprotein of aneurinibacillus thermoaerophilus l420-91t contain d-rhamnose and 3-acetamido-3,6-dideoxy-d-galactose, both of which are also constituents of lipopolysaccharides of gram-negative plant and human pathogenic bacteria. the two genes required for biosynthesis of the nucleotide-activated precursor gdp-d-rhamnose, gmd and rmd, were cloned, sequenced, and overexpressed in escherichia coli. the corresponding enzymes gmd and rmd were purified to hom ... | 2001 | 11096116 |
| structural heterogeneity in the core oligosaccharide of the s-layer glycoprotein from aneurinibacillus thermoaerophilus dsm 10155. | the surface layer glycoprotein of aneurinibacillus thermoaerophilus dsm 10155 has a total carbohydrate content of 15% (by mass), consisting of o-linked oligosaccharide chains. after proteolytic digestion of the s-layer glycoprotein bypronase e and subsequent purification of the digestion products by gel permeation chromatography, chromatofocusing and high-performance liquid chromatography two glycopeptide pools a and b with identical glycans and the repeating unit structure -->4)-alpha-l-rha p - ... | 1999 | 10406844 |
| complete glycan structure of the s-layer glycoprotein of aneurinibacillus thermoaerophilus gs4-97. | isolate gs4-97 was purified from an extraction juice sample of an austrian beet sugar factory and affiliated to the newly described species aneurinibacillus thermoaerophilus. it is closely related to the type strain of this species, a.thermoaerophilus l420-91(t), and possesses a square surface layer (s-layer) array composed of identical glycoprotein monomers as its outermost cell envelope component. by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the purified s-layer showed an appa ... | 1999 | 10089215 |
| control of downy mildew (pseudoperonospora cubensis) of greenhouse grown cucumbers with alternative biological agents. | in organic cucumber production infection with downy mildew (pseudoperonospora cubensis) is a major problem. plant extracts from glycyrrhiza glabra l. (licorice), a plant belonging to the family fabaceae, and salvia officinalis (sage) as well as cultures of the bacterium aneurinibacillus migulanus were investigated for efficacy of disease control under commercial growing conditions. contrary to bioassays, where sage extract and the microorganism showed highest activity, in the trials of 2008 g. g ... | 2010 | 21534461 |
| the structural basis for catalytic function of gmd and rmd, two closely related enzymes from the gdp-d-rhamnose biosynthesis pathway. | the rare 6-deoxysugar d-rhamnose is a component of bacterial cell surface glycans, including the d-rhamnose homopolymer produced by pseudomonas aeruginosa, called a-band o polysaccharide. gdp-d-rhamnose synthesis from gdp-d-mannose is catalyzed by two enzymes. the first is a gdp-d-mannose-4,6-dehydratase (gmd). the second enzyme, rmd, reduces the gmd product (gdp-6-deoxy-d-lyxo-hexos-4-ulose) to gdp-d-rhamnose. genes encoding gmd and rmd are present in p. aeruginosa, and genetic evidence indicat ... | 2009 | 19459932 |
| biosynthesis of dtdp-3-acetamido-3,6-dideoxy-alpha-d-glucose. | derivatives of 3-amino-3,6-dideoxyhexoses are widespread in nature. they are part of the repeating units of lipopolysaccharide o-antigens, of the glycan moiety of s-layer (bacterial cell surface layer) glycoproteins and also of many antibiotics. in the present study, we focused on the elucidation of the biosynthesis pathway of dtdp-alpha-d-quip3nac (dtdp-3-acetamido-3,6-dideoxy-alpha-d-glucose) from the gram-positive, anaerobic, thermophilic organism thermoanaerobacterium thermosaccharolyticum e ... | 2008 | 17941826 |
| the ability of aneurinibacillus migulanus (bacillus brevis) to produce the antibiotic gramicidin s is correlated with phenotype variation. | phenotype instability of bacterial strains can cause significant problems in biotechnological applications, since industrially useful properties may be lost. here we report such degenerative dissociation for aneurinibacillus migulanus (formerly known as bacillus brevis) an established producer of the antimicrobial peptide gramicidin s (gs). phenotypic variations within and between various strains maintained in different culture collections are demonstrated. the type strain, atcc 9999, consists o ... | 2007 | 17720841 |
| screening and distributing features of bacteria with hydantoinase and carbamoylase. | hydantoinase and carbamoylase are key biocatalysts for the production of optically pure amino acids from dl-5-substituted hydantoins (ssh). out of 364 isolated strains with hydantoinase and carbamoylase at 45 degrees c, 24 strains showed efficient hydantoinase and carbamoylase activities. among them, 17 produced l-amino acids, and 7 produced d-amino acids from both aromatic dl-5-benzylhydantoin and aliphatic dl-5-isopropylhydantoin. most of the strains that were able to form l-amino acid belonge ... | 2009 | 17498938 |
| characterisation and optimisation of three potential aerobic bacterial strains for kraft lignin degradation from pulp paper waste. | eight aerobic bacterial strains were isolated from pulp paper mill effluent sludge. out of eight through nutrient enrichment technique three potential aerobic bacterial strains itrc s(6), itrc s(7) and itrc s(8) were found capable to effectively degrade the kraft lignin (kl), a major byproduct of the chemical pulping process and main contributor to the colour and toxicity of effluent. further, these potential strains (itrc s(6), itrc s(7) and itrc s(8)) were biochemically characterised as gram v ... | 2007 | 17150240 |