| structure of the ockelbo virus genome and its relationship to other sindbis viruses. | ockelbo virus was first isolated in 1982 in sweden. it is the causal agent of disease in humans characterized by arthritis, rash, and fever and is antigenically very closely related to sindbis virus. we have determined the nucleotide and translated amino acid sequences of the prototype ockelbo virus isolate (82-5) to determine the relatedness of ockelbo virus to sindbis virus at the genomic level and clarify the taxonomic position of ockelbo virus within the alphavirus genus. the numbers of nucl ... | 1991 | 1673813 |
| [monoclonal antibodies to the karelian fever virus. the characteristics of their biological properties]. | biological properties of 6 variants of monoclonal antibodies (mab) to karelian fever virus, a member of the alpha-virus serocomplex sindbis-wee, produced by the available hybridomas. the productivity of hybridomas of the "karel" series in tissue culture and in cultivation as ascitic fluid was evaluated. among the antibodies analysed, all were specific to envelope proteins, of them 2 were against protein e2 and four against protein e1. comparison of mca biologic activity (neutralizing, antihemagg ... | 1990 | 1979458 |
| [a comparative analysis of the polypeptides of the the sindbis and karelian fever viruses]. | in pulse-chase experiments with karelian fever virus-infected cells, proteins were found with molecular weights of 130, 98, 78, and 62 kd of which the first, second and fourth were classified as polypeptide precursors of the structural proteins of virion. the molecular weights of proteins e1, e2 and c of 52, 47 and 34 kd, respectively, as well as isoelectric points of isolated glycoproteins (pi e1 = 6.3, pi e2 = 8.4) were similar in kfv (strain leiv-9298) and sindbis virus (strain ar339). the an ... | 1990 | 2176422 |
| [use of probes containing cloned genes of the karelian fever virus in detecting viral genetic material in infected cells by a molecular hybridization method]. | two plasmid dna-probes containing dna-replicas of kfv genes (clone 1-protein e1 gene, clone 9--proteins e1 and p1 genes of kfv) were used for detection of the genetic material of karelian fever virus (kfv) in the infected cells and study of the time course of accumulation of virus-specific rnas in the process of infection. the detection was performed by the method of rna:dna dot-hybridization. both probes were hybridized with kfv and sindbis virus rna in equal amounts--5 x 10(2) infected cells a ... | 1989 | 2728408 |
| [analysis of the primary structure of segments of the karelian fever virus genome]. | primary structure of two parts of karelian fever virus (kfv) genome (29-57 nt and 10507-11591 nt) cloned in recombinant plasmids has been studied and compared with that of sindbis (hrsp strain) and ockelbo viruses. fifty-four nucleotide substitutes were revealed in the sequenced parts of kfv genome including partially 5' and 3'-nontranslated sites ( a total of 1613 nucleotides, or approximately 13.8% of the genome length), in comparison with the sindbis virus prototype hrsp strain, this being in ... | 1995 | 8659171 |
| [recombination of alpha-viruses during mixed infection in cultured cells. formation of hybrid forms of rna of venezuelan equine encephalomyelitis virus and karelian fever virus in the region of genes for nucleocapsid and envelope proteins]. | a recombination of the genomes of vee and kf viruses, two alphaviruses of different serotypes, takes place in mixed infection of chick embryo fibroblasts and vero cells with vaccine strain vee-230 and kf-9298 strain. this recombination resulted in the production of recombinant virions with altered antigenic properties. polymerase chain reaction (pcr) rapidly and effectively detected the hybrid sites of the genome in recombinant viruses. short nucleotide sequences (approximately 20 n. p.) of a kn ... | 1996 | 8999680 |
| [comparative analysis of expression of genes for 2',5'-oligoadenylate synthetase and mrna for dsrna-protein kinase in human fibroblasts infected with the sindbis alphavirus and induced by alpha-interferon]. | expression of 2,5-oas and ds-protein kinase (ds-pk) in human fibroblast culture following treatment with alpha-if in a dose of 1000 u or challenge with karelian fever virus (kfv) (5 pfu/cell, 4 h.p.i.) was compared. a highly sensitive and rapid method, coupled rt-pcr, was used to detect mrna transcription levels. alpha-if had an expressed stimulating effect on 2,5-oas mrna level, whereas the virus markedly inhibited the synthesis of ds-pk mrna. | 1997 | 9297349 |
| sindbis-group alphavirus replication in periosteum and endosteum of long bones in adult mice. | several alphaviruses, including the sindbis-group viruses, ross river virus, o'nyong-nyong virus, and chikungunya virus, are associated with outbreaks of acute and persistent arthralgia and arthritis in humans. mechanisms underlying alphavirus-induced arthralgia and arthritis are not clearly understood, though direct viral replication within or around the affected joints is thought to contribute to disease. s.a.ar86 is a sindbis-group alphavirus closely related to the arthralgia-associated ockel ... | 2000 | 10982376 |
| transmission studies of a european sindbis virus in the floodwater mosquito aedes vexans (diptera: culicidae). | sindbis viruses are arthropod-borne viruses, which are maintained in nature in a culex mosquito-bird associated transmission cycle, but aedes species have been suspected as playing a role in infecting humans. in this study, we addressed the question whether or not germany's most abundant floodwater mosquito species aedes vexans (diptera, culicidae) can serve as an efficient vector for sindbis viruses. firstly, the overall susceptibility of ae. vexans was tested by intrathoracic inoculation of 40 ... | 2002 | 12141742 |
| [recombinant interferon-alpha suppression of karelian fever virus replication in human blood cells]. | the active replication of karelian fever virus (kfv) in human blood vessels and the protective activity of the russian agent reaferon were first shown. kfl was highly susceptible to interferon (ifn)-alpha. in control (uninfected) cells, reaferon caused low gene expressions of the ifn-dependent enzymes dsrna-dependent protein kinase and 2'5'-oligoadenylate synthetase, by exerting a little effect on the activity of its family genes. kfv suppressed the reaferon-induced gene expression of ifn-depend ... | 2016 | 22834144 |