polyphasic identification of bacillus and brevibacillus strains from clinical, dairy and industrial specimens and proposal of brevibacillus invocatus sp. nov.. | thirty-three clinical, dairy and industrial isolates of aerobic endospore-forming bacteria which were unreactive in routine identification tests were characterized genotypically by using amplified rdna restriction analysis (ardra), 16s rdna sequencing and dna-dna reassociation, and phenotypically by using fatty acid methyl ester (fame) analysis, sds-page of whole-cell proteins, api biotype 100 assimilation tests and 16 other routine phenotypic tests. three isolates were identified as strains of ... | 2002 | 12054263 |
biodegradation of crude oil using an efficient microbial consortium in a simulated marine environment. | ochrobactrum sp. n1, brevibacillus parabrevis n2, b. parabrevis n3 and b. parabrevis n4 were selected when preparing a mixed bacterial consortium based on the efficiency of crude oil utilization. a crude oil degradation rate of the n-series microbial consortium reached upwards of 79% at a temperature of 25 °c in a 3.0% nacl solution in the shake flask trial. in the mesocosm experiment, a specially designed device was used to simulate the marine environment. the internal tank size was 1.5 m (l)×0 ... | 2012 | 22498316 |
experimental investigation of microbiologically influenced corrosion of selected steels in sugarcane juice environment. | in the current study, ferritic stainless grades aisi 439 and aisi 444 were investigated as possible construction materials for machinery and equipment in the cane-sugar industry. their performance in corrosive cane-sugar juice environment was compared with the presently used low carbon steel aisi 1010 and austenitic stainless steel aisi 304. the tafel plot electrochemical technique was used to evaluate general corrosion performance. microbiologically influenced corrosion (mic) behaviour in sugar ... | 2013 | 23764955 |
biochemical characterization of a novel surfactant-stable serine keratinase with no collagenase activity from brevibacillus parabrevis cgmcc 10798. | dehairing is a high pollution process in leather industry. conventionally, the lime-sulfide mediated chemical process for dehairing would lead to the discharge of pollutants and corrosion of industrial equipment. concerning these problems, keratinase has become a promising candidate for dehairing process in recent years. in this study, a keratinase-producing bacterium was isolated from sheepfold soil and identified as brevibacillus parabrevis cgmcc 10798 based on the biochemical characteristics ... | 2016 | 27651275 |
brevibacillus gelatini sp.nov., isolated from hot spring in turkey. | two gram-positive, moderately thermophilic, endospore-forming, rod-shaped, motile bacteria were isolated from camkoy hot spring in the provinces of aydın, turkey and were characterized in order to determine their phylogenetic positions. these strains were designated as pdf4t and pdf10. 16s rrna gene sequence analysis revealed that the two strains belonged to the genus brevibacillus; strain pdf4t showed highest sequence similarity to strain pdf10 (99.5 %), brevibacillus brevis dsm 30t (98.9 %), b ... | 2015 | 26585900 |
antifungal membranolytic activity of the tyrocidines against filamentous plant fungi. | the tyrocidines and analogues are cyclic decapeptides produced by brevibacillus parabrevis with a conserved sequence of cyclo(d-phe(1)-pro(2)-x(3)-x(4)-asn(5)-gln(6)-x(7)-val(8)-x(9)-leu(10)) with trp(3,4)/phe(3,4) in the aromatic dipeptide unit, lys(9)/orn(9) as their cationic residue and tyr (tyrocidines), trp (tryptocidines) or phe (phenicidines) in position 7. previous studies indicated they have a broad antifungal spectrum with the peptides containing a tyr residue in position 7 being more ... | 2016 | 27328781 |
the heat resistance and spoilage potential of aerobic mesophilic and thermophilic spore forming bacteria isolated from chinese milk powders. | the propensity for aerobic bacilli and allied genera to produce highly heat-resistant spores and thermally stable spoilage enzymes are major bacteriological issues faced by the dairy industry. most of the enzymes are able to survive any heat treatment applied during the manufacture of milk powders and have the potential to remain active in milk powders and other dairy products during storage, and may explain some of the sensory and functionality defects reported in dairy products. despite many r ... | 2016 | 27657656 |
[identification of pyrene degrading strains and the degrading characteristics research]. | three bacterial strains j1, j2, j3 which could use pyrene as the sole carbon and energy sources were isolated from soils contaminated with polycyclic aromatic hydrocarbons (pahs) by enrichment culture. the strains were identified as pseudomonas aeruginosa, flavobacterium mizutaii, brevibacillus parabrevis according to the results of morphology, physiology and the phylogenetical analyses of 16s rdna sequence. it was observed that the three strains could use pyrene at the concentrations of 50, 100 ... | 2010 | 20329546 |
identification and characterization of microorganisms from earthworm viscera for the conversion of fish wastes into liquid fertilizer. | five bacteria isolated from earthworm viscera and identified as brevibacillus agri, bacillus cereus, bacillus licheniformis, and brevibacillus parabrevis by 16s rrna sequencing were employed in the conversion of fish wastes generated from a restaurant specializing in sliced raw fish into fertilizer. within 120h after inoculation of autoclaved fish waste with 5.15 x 10(5) cfu ml(-1) mixed isolates, the amount of dry sludge decreased from 29.4 to 0.2g, the ph changed from 7.05 to 5.70, and the cel ... | 2010 | 20189380 |
enhancement of linear gramicidin expression from bacillus brevis atcc 8185 by casein peptide. | bacillus brevis (brevibacillus parabrevis) atcc 8185 synthesizes two kinds of antibiotic peptides, cyclopeptide tyrocidine and linear gramicidin. the production of linear gramicidin can be induced by the standard method (using a skim milk medium for pre-culture and beef broth for the main culture) employed for the induction of tyrocidine. in this study, we tried to determine the optimal growth medium for b. brevis atcc 8185 for synthesizing linear gramicidin. the yield of linear gramicidin produ ... | 2005 | 15849407 |
proposal for two new genera, brevibacillus gen. nov. and aneurinibacillus gen. nov. | 16s rrna gene sequences of the type strains of 11 species belonging to the bacillus brevis and bacillus aneurinolyticus groups were determined. on the basis of the results of gene sequence analyses, these species were separated into two clusters. the b. brevis cluster included 10 species, namely, bacillus brevis, bacillus agri, bacillus centrosporus, bacillus choshinensis, bacillus parabrevis, bacillus reuszeri, bacillus formosus, bacillus borstelensis, bacillus laterosporus, and bacillus thermo ... | 1996 | 8863420 |
characterization of bacillus brevis with descriptions of bacillus migulanus sp. nov., bacillus choshinensis sp. nov., bacillus parabrevis sp. nov., and bacillus galactophilus sp. nov. | thirty-five bacillus brevis strains obtained from culture collections, including protein-producing isolates, were taxonomically studied by using numerical analysis, dna base composition, and dna-dna hybridization. six dna relatedness groups were represented, and these groups correlated well with clusters based on the numerical analysis. the b. brevis strains were separated into b. brevis sensu stricto, four new species, and an unidentified species of the genus bacillus. bacillus migulanus sp. no ... | 1993 | 8494737 |
determination of cypermethrin degradation potential of soil bacteria along with plant growth-promoting characteristics. | the pyrethroid insecticide cypermethrin is in extensive use since 1980s for insect control. however, its toxicity toward aquatic animals and humans requires its complete removal from contaminated areas that can be done using indigenous microbes through bioremediation. in this study, three bacterial strains isolated from agricultural soil and identified as acinetobacter calcoaceticus mcm5, brevibacillus parabrevis fcm9, and sphingomonas sp. rcm6 were found highly efficient in degrading cypermethr ... | 2015 | 25194282 |
combination of culture-independent and culture-dependent molecular methods for the determination of bacterial community of iru, a fermented parkia biglobosa seeds. | in this study, bacterial composition of iru produced by natural, uncontrolled fermentation of parkia biglobosa seeds was assessed using culture-independent method in combination with culture-based genotypic typing techniques. pcr-denaturing gradient gel electrophoresis (dgge) revealed similarity in dna fragments with the two dna extraction methods used and confirmed bacterial diversity in the 16 iru samples from different production regions. dna sequencing of the highly variable v3 region of the ... | 2012 | 23316189 |
brevibacillus ginsengisoli sp. nov., a denitrifying bacterium isolated from soil of a ginseng field. | a gram-positive, rod-shaped, spore-forming bacterium, gsoil 3088t, was isolated from soil from a ginseng field in pocheon province in south korea and characterized in order to determine its taxonomic position. on the basis of 16s rrna gene sequence similarity, strain gsoil 3088t was shown to belong to the family paenibacillaceae, being related to brevibacillus centrosporus (96.6%), brevibacillus borstelensis (96.3%), brevibacillus parabrevis (96.1%), brevibacillus formosus (96.1%), brevibacillus ... | 2006 | 17082408 |
reclassification of brevibacillus brevis strains ncimb 13288 and dsm 6472 (=nrrl nrs-887) as aneurinibacillus danicus sp. nov. and brevibacillus limnophilus sp. nov. | comparison of the hypervariable region (269-279 bases in length) at the 5' end of the 16s rdna sequences of 29 bacterial strains that were identified previously as brevibacillus brevis showed that 13 strains clustered with aneurinibacillus species, eight strains clustered with bacillus species and eight strains clustered with brevibacillus species. based on dna-dna hybridization results, 27 strains, not including [brevibacillus brevis] ncimb 13288 and [brevibacillus brevis] dsm 6472, were reiden ... | 2004 | 15023954 |