| brevibacillus ginsengisoli sp. nov., a denitrifying bacterium isolated from soil of a ginseng field. | a gram-positive, rod-shaped, spore-forming bacterium, gsoil 3088t, was isolated from soil from a ginseng field in pocheon province in south korea and characterized in order to determine its taxonomic position. on the basis of 16s rrna gene sequence similarity, strain gsoil 3088t was shown to belong to the family paenibacillaceae, being related to brevibacillus centrosporus (96.6%), brevibacillus borstelensis (96.3%), brevibacillus parabrevis (96.1%), brevibacillus formosus (96.1%), brevibacillus ... | 2006 | 17082408 |
| [cloning of the restriction-modification genes of bacillus centrosporus in escherichia coli]. | a genomic library of bacillus centrosporus was obtained using pbr327 as a vector. the total plasmid dna of the library was cleaved by the bcni restriction endonuclease and then transformed in escherichia coli rr1. two clones possessing restriction and dna modification profiles of bcni were identified among the transformants. their respective plasmids were 13.3 and 9.05 kbp in size. restriction mapping of both plasmids showed each of them to contain two sites for hindiii and one for both eco31i a ... | 1988 | 2834268 |
| description of heterotrophic bacteria occurring in paper mills and paper products. | to isolate aerobic mesophilic bacilli and thermophilic bacteria from different paper mill samples and to evaluate their potential harmfulness. | 2004 | 15546414 |
| polyphasic identification of bacillus and brevibacillus strains from clinical, dairy and industrial specimens and proposal of brevibacillus invocatus sp. nov.. | thirty-three clinical, dairy and industrial isolates of aerobic endospore-forming bacteria which were unreactive in routine identification tests were characterized genotypically by using amplified rdna restriction analysis (ardra), 16s rdna sequencing and dna-dna reassociation, and phenotypically by using fatty acid methyl ester (fame) analysis, sds-page of whole-cell proteins, api biotype 100 assimilation tests and 16 other routine phenotypic tests. three isolates were identified as strains of ... | 2002 | 12054263 |
| circular permutation of dna cytosine-n4 methyltransferases: in vivo coexistence in the bcni system and in vitro probing by hybrid formation. | sequence analysis of the bcni restriction-modification system from bacillus centrosporus revealed four open reading frames (bcnic, bcnir, bcnib and bcnia) that are arranged as two converging collinear pairs. one pair encodes a putative small regulatory protein, c.bcni, and the restriction endonuclease r.bcni. the other two gene products are the dna cytosine-n4 methyltransferases m.bcnia and m.bcnib, which differ by circular permutation of conserved sequence motifs. the bcni methyltransferases ar ... | 2002 | 11917015 |
| a pair of single-strand and double-strand dna cytosine-n4 methyltransferases from bacillus centrosporus. | sequence analysis of the bcni restriction-modification system revealed the presence of an open reading frame encoding a second cytosine-n4 methyltransferase, m.bcnia, in the vicinity of the genes specifying the previously characterized cytosine-n4 methyltransferase m.bcnib and restriction endonuclease r.bcni. both methyltransferases were purified from the e. coli cells expressing the individual genes, and their enzymatic efficiencies in vitro were compared with a variety of dna substrates. both ... | 1998 | 9628356 |
| proposal for two new genera, brevibacillus gen. nov. and aneurinibacillus gen. nov. | 16s rrna gene sequences of the type strains of 11 species belonging to the bacillus brevis and bacillus aneurinolyticus groups were determined. on the basis of the results of gene sequence analyses, these species were separated into two clusters. the b. brevis cluster included 10 species, namely, bacillus brevis, bacillus agri, bacillus centrosporus, bacillus choshinensis, bacillus parabrevis, bacillus reuszeri, bacillus formosus, bacillus borstelensis, bacillus laterosporus, and bacillus thermo ... | 1996 | 8863420 |
| cloning of the modification methylase gene of bacillus centrosporus in escherichia coli. | the gene specifying a sequence-specific modification methylase of bacillus centrosporus has been cloned in escherichia coli using the restriction endonuclease hindiii and the plasmid pbr322. the selection was based on detection of new methylation properties rendering recombinant plasmids carrying the methylase gene nonsusceptible to bcni endonuclease cleavage. the presence of a 3.2-kb hindiii fragment in either orientation conferred bcni resistance on the recombinant plasmids. these results sugg ... | 1982 | 6299887 |
| a new restriction endonuclease bcni from bacillus centrosporus rfl 1. | | 1982 | 6277689 |
| isolation and some properties of the restriction endonuclease bcni from bacillus centrosporus. | a specific type-ii restriction endonuclease bcni from bacillus centrosporus has been purified to electrophoretic homogeneity in three chromatographic steps. around 15 micrograms of such a preparation can be isolated from 1 g of the cell paste. the yield of the enzyme is higher than that of any type-ii restriction endonuclease so far reported. the molecular weight of the enzyme determined by gel filtration and polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate equals 27 ... | 1982 | 6277627 |