| effects of crude extracts of various plants on infectious bovine rhinotracheitis virus-plaque production. | extracts of 28 plants were tested without demonstable antiviral activity in an agar-overlay plaque-reduction antiviral assay system, using infectious bovine rhinotracheitis virus and bovine endocardial cell cultures. ethanolic extract of narcissus tazetta l bulb elicited antiviral activity by inhibition of viral plaque formation. antiviral activity was demonstrated against infectious bovine rhinotracheitis and equine rhinopneumonitis viruses. narcissus tazetta l bulb did not directly inactivate ... | 1976 | 176907 |
| inhibition of dna polymerase of avian myeloblastosis virus by an alkaloid extract from narcissus tazetta l. | | 1973 | 4123212 |
| isolation and characterization of a mannose-binding lectin from leaves of the chinese daffodil narcissus tazetta. | a mannose-binding lectin was isolated from leaves of the chinese daffodil narcissus tazetta (family amaryllidaceae) using a procedure that comprised extraction with aqueous buffer, ammonium sulfate precipitation, ion exchange chromatography on deae-cellulose, affinity chromatography on affi-gel blue gel and mannose-agarose, and fplc-gel filtration on superose 12. the lectin was adsorbed on mannose-agarose and unadsorbed on deae-cellulose and affi-gel blue gel. it was an unglycosylated homodimer ... | 1998 | 10099780 |
| lectins from bulbs of the chinese daffodil narcissus tazetta (family amaryllidaceae). | the isolation of three lectins with similar n-terminal amino acid sequences from the bulbs of the chinese daffodil narcissus tazetta was achieved. the isolation protocol involved ion exchange chromatography on deae-cellulose, affinity chromatography on mannose-agarose, and fast protein liquid chromatography-gel filtration on superose 12. the lectins were all adsorbed on mannose-agarose and demonstrated a single band with a molecular weight of 13 kda in sds-polyacrylamide gel electrophoresis and ... | 2000 | 11012085 |
| isolation and characterization of a bioactive mannose-binding protein from the chinese chive allium tuberosum. | a mannose-binding protein was isolated from two different cultivars of the chinese chive allium tuberosum by extraction with 0.2 m nacl, ammonium sulfate precipitation, and affinity chromatography on mannose agarose and fetuin agarose. it exhibited hemagglutinating activity toward rabbit erythrocytes. the lectin (agglutinin) was adsorbed on the mannose-agarose column, but not on the fetuin-agarose column. this a. tuberosum lectin (atl) is unglycosylated, and not sialic acid binding. lectins isol ... | 2002 | 11829630 |
| gene expression of immunomodulatory cytokines induced by narcissus tazetta lectin in the mouse. | the immunomodulation of narcissus tazetta lectin (ntl) on the induction of gene expression of cytokines in the mouse was studied using specific cytokine primers, total rna isolated from mouse splenocytes and macrophages, and reverse transcription-polymerase chain reaction (rt-pcr). for comparison, a fungal antimitogenic lectin from agaricus bisporus (abl) was used to test and compare the acute (kinetic) induction of cytokine gene expression. ntl was able to induce the expression of il-1beta, tnf ... | 2002 | 11989721 |
| antimalarial activity screening of some alkaloids and the plant extracts from amaryllidaceae. | four groups of amaryllidaceae alkaloids, namely lycorine-, crinine-, tazettine-, and galanthamine-type, as well as plant extracts of the amaryllidaceae plants (pancratium maritimum, leucojum aestivum, and narcissus tazetta ssp. tazetta) growing in turkey were evaluated in vitro for their ability to inhibit plasmodium falciparum growth by a high-throughput screening method with a 96-well microtiter plate. all four groups of alkaloids exhibited antimalarial activity at different potencies. 6-hydro ... | 2003 | 14669261 |
| first report of a glutamine-rich antifungal peptide with immunomodulatory and antiproliferative activities from family amaryllidaceae. | this represents the first report of purification of a glutamine-rich antifungal peptide from family amarylliaceace. the peptide, designated as nartazin, was purified from the bulbs of the chinese daffodil narcissus tazetta var. chinensis by means of ion-exchange chromatography and affinity chromatography. its molecular mass was 7.1kda, as determined by sds-page and gel filtration. nartazin stimulated proliferation of mouse splenocytes and bone marrow cells but inhibited proliferation of leukemia ... | 2004 | 15522215 |
| characterisation of an isolate of narcissus degeneration virus from chinese narcissus (narcissus tazetta var. chinensis). | a potyvirus from chinese narcissus was transmitted mechanically to three species of narcissus and to lycoris radiata but not to 22 other test species. in western blot, the coat protein reacted strongly with narcissus degeneration virus (uk isolate) antiserum. antiserum raised to the chinese virus did not react with eighteen other potyviruses. the complete nucleotide sequence (9816 nt) had the typical genome organisation for a member of the genus potyvirus. sequence comparisons and phylogenetic a ... | 2007 | 16932980 |
| ritual plants of muslim graveyards in northern israel. | this article surveys the botanical composition of 40 muslim graveyards in northern israel, accompanied by an ethnobotanical study of the folkloristic traditions of the use of these plants in cemeteries. three groups of plants were found to be repeated systematically and were also recognized for their ritual importance: aromatics herbs (especially salvia fruticosa and rosmarinus officinalis), white flowered plants (mainly narcissus tazetta, urginea maritima, iris spp. and pancratium spp.) and cup ... | 2006 | 16961931 |
| development-specific association of amyloplasts with microtubules in scale cells of narcissus tazetta. | narcissus tazetta is one of the major geophyte crops worldwide, but little is known about its cell biology. the narcissus storage organ was studied by monitoring scale cell biology during the growth stage and dormancy, and it was found that amyloplasts gradually increased in size and reached a maximum at dormancy. in parallel, microtubules changed their organisation: during the growth phase (february to march) they were oblique; during april and may, microtubules formed a network with round "hol ... | 2007 | 17458630 |
| the plant mannose-binding lectin ntl preserves cord blood haematopoietic stem/progenitor cells in long-term culture and enhances their ex vivo expansion. | ex vivo expansion of haematopoietic stem and progenitor cells in cytokine combinations is effective in promoting differentiation and proliferation of multilineage progenitor cells, but often results in reduction of self-renewable stem cells. this study investigated the effect of a mannose-binding lectin, ntl, purified from narcissus tazetta var. chinensis, on prolonged maintenance and expansion of cord blood cd34+ cells. our results showed that the presence of ntl or flt-3 ligand (fl) significan ... | 2008 | 17908183 |
| isolation, characterization, molecular cloning and modeling of a new lipid transfer protein with antiviral and antiproliferative activities from narcissus tazetta. | a fetuin-binding peptide with a molecular mass of about 9kda (designated ntp) was isolated and purified from the bulbs of chinese daffodil, narcissus tazetta var. chinensis l., by gel filtration and high-performance liquid chromatography, after removing the mannose-binding proteins by mannose-agarose column. molecular cloning revealed that ntp contained an open reading frame of 354bp encoding a polypeptide of 118 amino acids which included a 26-amino-acid signal peptide. an analysis of the deduc ... | 2008 | 18824058 |
| antimicrobial, cytotoxicity and phytochemical screening of jordanian plants used in traditional medicine. | antimicrobial activity and cytotoxicity of fifty one extracts of different parts of 14 plants were studied. ethanol, methanol, aqueous, butanol, and n-hexane extracts were tested against three gram negative, two gram positive bacteria, and two fungi. cytotoxicity and phytochemical screening were determined using mtt and tlc assays, respectively. of the fifty one extracts, twenty two showed activities against different microorganisms with mics ranging from 62.5 to 1000 microg/ml. the highest acti ... | 2010 | 20336015 |
| narcissus tazetta lectin shows strong inhibitory effects against respiratory syncytial virus, influenza a (h1n1, h3n2, h5n1) and b viruses. | a mannose-binding lectin (narcissus tazetta lectin [ntl]) with potent antiviral activity was isolated and purified from the bulbs of the chinese daffodil narcissus tazetta var. chinensis, using ion exchange chromatography on diethylaminoethyl (deae)-cellulose, affinity chromatography on mannose-agarose and fast protein liquid chromatography (fplc)-gel filtration on superose 12. the purified lectin was shown to have an apparent molecular mass of 26 kda by gel filtration and 13 kda by sds-page, in ... | 2010 | 20413914 |
| oxidative stress and mitochondrial dysfunctions are early events in narciclasine-induced programmed cell death in tobacco bright yellow-2 cells. | narciclasine (ncs) is a plant growth inhibitor isolated from the secreted mucilage of narcissus tazetta bulbs. it is a commonly used anticancer agent in animal systems. in this study, we provide evidence to show that ncs also acts as an agent in inducing programmed cell death (pcd) in tobacco bright yellow-2 (tby-2) cell cultures. ncs treatment induces typical pcd-associated morphological and biochemical changes, namely cell shrinkage, chromatin condensation and nuclear dna degradation. to inves ... | 2012 | 21916896 |
| hexacyclopeptides secreted by an endophytic fungus fusarium solani n06 act as crosstalk molecules in narcissus tazetta. | the basis of chemical crosstalk in plants and associated endophytes lies in certain so-called communication molecules that are responsible for plant-microbe and microbe-microbe interactions. consequently, elucidating the factors that affect the nature, distribution, and amount of these molecules and how they impact the interaction among endophytes and associated organisms is essential to understand the true potential of endophytes. in the present study, we report the discovery of nine hexacyclop ... | 2015 | 25958995 |
| infection of narcissus roots by aphelenchoides subtenuis. | the widespread destruction of commercially grown bulbs of narcissus tazetta papyraceus (paper white) has been reported in israel. this phenomenon is usually characterized by a premature yellowing of the foliage, accompanied by root rot and dark, sunken basal plates. this study confirmed thataphelenchoides subtenuis is the main cause of the basal plate disease of narcissus. in contrast to other aphelenchoides species, which feed on stems or leaves, a. subtenuis penetrates narcissus roots. in our ... | 1993 | 19279798 |
| evolutionary rates and genetic diversities of mixed potyviruses in narcissus. | there is no attempt to evaluate evolutionary rates, timescales and diversities of viruses collected from mixedly infected hosts in nature. plants of the genus narcissus are a monocotyledon and are susceptible to several viruses. in this study, narcissus plants (narcissus tazetta var. chinensis) showing mosaic or striping leaves were collected in japan, and these were investigated for potyvirus infections using potyvirus-specific primers. individual narcissus plants were found frequently to be mi ... | 2016 | 27590715 |
| secretory expression and characterization of chinese narcissus gna-like lectin in pichia pastoris. | narcissus tazetta lectin (ntl) is a gna-like lectin, which has a wide potential application in medicine, agriculture, and glycobiology. in the present paper, the codon-optimized ntl gene was transformed into the yeast pichia pastoris; sds-page gel and western blotting analysis revealed that the recombinant lectin was expressed successfully in pichia yeast. the similarity between the recombinant ntl and the native ntl was confirmed by circular dichroism (cd) and hemagglutination assay further. in ... | 2017 | 28130769 |
| ft-like nft1 gene may play a role in flower transition induced by heat accumulation in narcissus tazetta var. chinensis. | the low-temperature flowering-response pathway, used as an inductive stimulus to induce flowering in plant species from temperate regions in response to cold temperature, has been extensively studied. however, limited information is available on the flower transition of several bulbous species, which require high temperature for flower differentiation. narcissus tazetta var. chinensis (chinese narcissus) exhibits a 2 year juvenile phase, and flower initiation within its bulbs occurs during summe ... | 2013 | 23303875 |
| narciclasine inhibits the responses of arabidopsis roots to auxin. | the plant hormone auxin plays a central role in the regulation of plant growth and development, as well as in responses to environmental stimuli. narciclasine (ncs, an amaryllidaceae alkaloid) isolated from narcissus tazetta bulbs has a broad range of inhibitory effects on plants. in this study, the role of ncs in responses to auxin in arabidopsis thaliana roots was investigated. we demonstrated the inhibitory effects of ncs on auxin-inducible lateral root formation, root hair formation, primary ... | 2012 | 22476291 |
| the flavonol glycosides in the fruit of pyrus communis l. cultivar bon chrétien. | 1. two new flavonol glycosides were isolated from the fruit of pyrus communis l. cultivar bon chrétien. these were identified as isorhamnetin 3-rhamnogalactoside and a derivative of isorhamnetin 3-glucoside which was associated (possibly acylated) with an unknown aliphatic organic acid. 2. the melting point of isorhamnetin 3-glucoside isolated from bon chrétien pears is different from that of isorhamnetin 3-glucoside previously isolated from argemone mexicana and calendula officinalis. 3. isorha ... | 1965 | 16749105 |
| molecular cloning and spatiotemporal expression of an apetala1/fruitfull-like mads-box gene from the orchid (cymbidium faberi). | in order to identify genes involved in floral transition and development of the orchid species, a full-length apetala1/fruitfull-like (ap1/ful-like) mads box cdna was cloned from cymbidium faberi (c. faberi) sepals and designated as c. faberi apetala1-like (cfap11], jq031272.1). the deduced amino acid sequence of cfap11 shared 84% homology with a member of the ap1/ful-like group of mads box genes (ay927238.1, dendrobium thyrsiflorum ful-like mads box protein 3 mrna). phylogenetic analysis shows ... | 2013 | 23697165 |
| cloning and functional characterization of a gna-like lectin from chinese narcissus (narcissus tazetta var. chinensis roem). | a full-length cdna encoding narcissus tazetta lectin (ntl) was isolated from chinese narcissus (n. tazetta var. chinensis roem). the open reading frame (orf) was 519 bp long and encoded 172 amino acids with a theoretical isoelectric point of 5.27 and a calculated molecular mass of 18.6 kda. conserved domain analysis indicated that it possessed three d-(+)-mannose-binding sites, presumed to be similar to those of galanthus nivalis agglutinin (gna)-like lectins. a recombinant (glutathione s-transf ... | 2011 | 21261630 |