| reaction of the anastral mitotic apparatus of endosperm cells of the plant leucojum aestivum with antibodies to tubulin from porcine brain as revealed by immunofluorescence microscopy. | structures binding an antibody against tubulin from porcine brain were localized in the giant anastral mitotic apparatus of endosperm cells of the monocotyledonous plant, leucojum aestivum, by indirect immunofluorescence microscopy. both continuous and chromosomal spindle fibers were strongly stained. postive fluorescence was also noted in polar cap regions and, in prometaphase stages, to some extent at the fragmented nuclear envelope. intermingling and branching of subfiber elements was frequen ... | 1977 | 346233 |
| radioimmunoassay for the quantitative determination of galanthamine. | a radioimmunoassay for the quantitation of pmol amounts of the therapeutically important amaryllidaceae alkaloid, galanthamine, has been developed. the antiserum was raised against a conjugate of galanthamine-2-o-hemisuccinate-bovine serum albumin. the measuring range of the assay extends from 0.5 to 100 ng of galanthamine, and as little as 3.5 pmol may be detected. the antiserum is highly specific for galanthamine, showing practically no cross reactivity with different representatives of the mo ... | 1990 | 2356245 |
| antimalarial activity screening of some alkaloids and the plant extracts from amaryllidaceae. | four groups of amaryllidaceae alkaloids, namely lycorine-, crinine-, tazettine-, and galanthamine-type, as well as plant extracts of the amaryllidaceae plants (pancratium maritimum, leucojum aestivum, and narcissus tazetta ssp. tazetta) growing in turkey were evaluated in vitro for their ability to inhibit plasmodium falciparum growth by a high-throughput screening method with a 96-well microtiter plate. all four groups of alkaloids exhibited antimalarial activity at different potencies. 6-hydro ... | 2003 | 14669261 |
| enzyme immunoassay for the quantitative determination of galanthamine. | an enzyme immunoassay for the quantitation of fmol amounts of the therapeutically important amaryllidaceae alkaloid, galanthamine, was established. the antiserum was raised against a conjugate of galanthamine-2- o-hemisuccinate-bovine serum albumin. the antibodies used were isolated and purified by rivanol treatment with subsequent (nh (4)) (2)so (4) precipitation. the measuring range of the assay extends from 2 to loo pg of galanthamine, and as little as 3.5 fmol may be detected. the antibodies ... | 1993 | 17236005 |
| antiviral activities in extracts of turkish medicinal plants. | a total of 16 ethanol extracts of turkish medicinal plants were evaluated for antiviral activities against herpes simplex virus (hsv) and sindbis virus (sinv). extracts of galanthus elwesii and rheum ribes showed the most potent anti-hsv activities, while six other extracts had weaker activities. galanthus elwesii and leucojum aestivum were the most potent anti-sinv extracts with four others showing weaker activities. in total, five extracts were active against both viruses, three were selective ... | 2000 | 21214457 |
| antifungal and antibacterial activity of extracts and alkaloids of selected amaryllidaceae species. | alkaloidal extracts of six selected species of amaryllidaceae were studied with respect to their antibacterial and anti-yeast activity and their alkaloidal fingerprint. twenty-five alkaloids were determined by gc/ms, and sixteen of them identified from their mass spectra, retention times and retention indexes. in the antimicrobial assay, gram-negative escherichia coli and gram-positive staphylococcus aureus were used, along with isolates of the human pathogenic yeasts candida albicans, c. glabra ... | 2015 | 26594752 |
| cloning and characterization of a norbelladine 4'-o-methyltransferase involved in the biosynthesis of the alzheimer's drug galanthamine in narcissus sp. aff. pseudonarcissus. | galanthamine is an amaryllidaceae alkaloid used to treat the symptoms of alzheimer's disease. this compound is primarily isolated from daffodil (narcissus spp.), snowdrop (galanthus spp.), and summer snowflake (leucojum aestivum). despite its importance as a medicine, no genes involved in the biosynthetic pathway of galanthamine have been identified. this absence of genetic information on biosynthetic pathways is a limiting factor in the development of synthetic biology platforms for many import ... | 2014 | 25061748 |
| [experimental cultivation of the plants containing galanthamine at izu. (1) general growth of shokiran (lycoris aurea herb.), natsuzuisen (l. squamigera maxim.), snowflake (leucojum aestivum l.), and snowdrop (galanthus nivalis l.), 1961 to 1962]. | | 1963 | 14162015 |