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parasites in bulinus senegalensis (mollusca: planorbidae) and their detection.isoelectric focusing studies on enzyme variation between populations of the snail bulinus senegalensis revealed that parasitic infections in the snails contributed additional bands of enzyme activity, particularly in the glucose phosphate isomerase (gpi) and malate dehydrogenase (mdh) systems. the patterns due to the parasite enzymes were, in most cases, clearly distinct from those of the host and different from each other. parasites encountered included schistosoma haematobium, s. bovis, paramp ...197944358
[changes in the composition of bovine serum during invasion by distomata genus paramphistomum and fasciola hepatica]. 197549982
trials with rafoxanide. 8. efficacy of an injectable solution against trematodes and nematodes in cattle.four experiments are described in which the efficacy of an experimental 5% injectable solution of rafoxanide was evaluated against various adult and immature helminths in cattle. subcutaneous injection at a dosage of 3 mg/kg live mass resulted in the following reductions in mean worm burdens: adult fasciola hepatica, 82,6%; adult fasciola gigantica, 99,8% immature paramphistomum microbothrium, 10,1% adult haemonchus placei, 99,6%, third stage h. placei, 73,7%; adult bunostomum phlebotomum, 99,8% ...1977144191
phosphatase systems in paramphistomum explanatum fischoeder, 1901.extracts of adult paramphistomum explanatum have been shown to contain high concentration of acid phosphomonoesterase with maximum activity at ph 4.5. the enzyme has been characterized by an exhibition of an unexpected increase in the inhibitory action of a mercury at 1 mm concentration by edta. with a lower concentration of mercury (0.1 mm and below) edta gave partial protection against inhibition. different concentrations of magnesium and cobalt activated the enzyme while fluoride, copper, ars ...1975238347
effect of some--sh and other reagents on aspartate aminotransferase and l-alanine aminotransferase of paramphistomum explanatum fischoeder, 1901.studies on aspartate aminotransferase (got) and l-alanine aminotransferase (gpt) of paramphistomum explanatum have shown that gpt activity has more than twice the activity of got. the effect os some--sh reagents like cadmium, mercury, silver and iodoacetamide revealed that both enzymes were inhibited except that got was insensitive to cadmium ions. gpt was found to be much more sensitive to--sh reagents than got. there was unusual reaction to the two thiols used, cysteine and mercaptoethanol. cy ...1978418589
annual maturation of paramphistomum cervi (trematoda: paramphistomatidae) in moose, alces alces l. 1979540274
[dissemination of fasciola hepatica in cattle of novara province].the diffusion of f. hepatica in the cattle of the novara province was studied by means of a necroscopic survey on 2184 regularly slaughtered animals and a copro-microscopic survey on 5063 feaces samples picked up from 162 breedings farms. the 28.40% of the slaughtered animals resulted infected with f. hepatica. the parasite also resulted present in 52.74% of the examined breeding farms. a large diffusion of paramphistomum sp. was shown, too. the diffusion areas of these trematodes may be seen in ...1978553277
[contribution to the biology and pathogenicity of domestic omasal leech paramphistomum cervi. 1. developmental stages in the free state and in the intermediary host]. 1977588222
trematodes of the genus paramphistomum fischoeder, 1901 in ruminants of poland. 1977589497
schistosoma mattheei in the ox: the serological response.thirty friesian steers were infected with schistosoma mattheei and the antibody response was followed for up to 76 weeks by the complement fixation (cf), indirect haemagglutination (ih) and indirect immunofluorescent (if) tests. cf and if antibodies rose to a peak at about 25 weeks and then fell, while ih antibodies rose more slowly and remained high. peak ih and if titres were proportional to the level of infection. peak cf titres were reduced in animals on a low plane of nutrition. there was a ...1977605296
[contribution to the biology and pathogenicity of the endemic rumen flatworm paramphistomum cervi. 2. occurrence in pasture cattle grazing in marshy areas]. 1978629734
[biology and pathogenicity of the domestic ruminal fluke paramphistomum cervi. 3. development in cattle, sheep and deer]. 1978629739
experimental studies on the suitability of aquatic snails as intermediate hosts for paramphistomum cervi in iraq.three of the most common species of aquatic snails in iraq were examined for their susceptibility to infection with paramphistomum cervi. bulinus truncatus was the only susceptible species. no development occurred in physa acuta or lymnaea lagotis euphratica. these findings and a previous field observation that infection of b. truncatus with amphistome larvae is not uncommon indicate that b. truncatus is an intermediate host of p. cervi in iraq. young snails of b. truncatus were more susceptible ...1978666386
histochemical distribution of acetyl & butyryl cholinesterase in the tissues of a trematode paramphistomum cervi. 1978721155
[life cycle of paramphistomum togolense n. sp. (trematoda, paramphistomidae)].mature worms were obtained from a sheep experimentally infested with metacercariae issued from cercariae emitted by naturally infested snails: bulinus (bulinus) forskalli collected near lomé (togo). miracidia were used for infection of these laboratory-reared snails which gave numerous cercariae. larval stages are studied. morphological and histological characters of the adult and the very peculiar shape of the sporocyst distinguish this trematode from the other known species of the genus. the s ...1978742803
life-cycle and geographical distribution of paramphistomum daubneyi dinnik, 1962 (trematoda: paramphistomata). 1977754531
[clinical examination of the blood of roe deer (capreolus capreolus l.) and fallow deer (dama dama l.) naturally invaded by parasites].roe-deer (capreolus capreolus l. -- five animals) and fallow deer (dama dama l. -- eleven animals) of both sexes and at different age were subject to blood examination. the deer were killed in several preserves in bohemia during autumn and winter shootings in two years. the following blood values were determined: erythrocyte count, leucocyte count, hemoglobin content, hematocrit values, mcv, mch, mchc, white blood picture, total protein, its fractions (albumin, alpha-, beta-, and gamma-globulin) ...1975809892
[clinical examination of the blood of red deer (cervus elaphus l.) naturally infested with parasites].in six animals hunted and four immobilized animals of red deer (cervus elaphus l.) of both sexes and of different age, kept at three game preserves in bohemia, the psychological values were ascertained: the number of erythrocytes and leucocytes, the hemoglobin content, the hematocrit, mcv, mch, mchc, the white blood count, the total serum protein, the fractions: albumin, alpha-, beta- and gamma-globulin, the activity of sgot, and sgpt, the alkaline phosphatase and the metabolism of calcium, phos ...1976820034
enteric paramphistomiasis in yankasa sheep.an outbreak of acute enteric paramphistomiasis was observed among yankasa (indigenous) sheep that were herded with cattle on the university farm at samaru in february 1973. mortality was approximately thirty to forty per cent. the most important clinical signs were anorexia, unthriftiness and profuse foetid diarrhoea soiling the perianal region and hind legs. diagnosis was based on the post-mortem findings, clinical signs and presence of conical flukes identified as paramphistomum microbothrium.1977906085
[studies of the decontamination of the sewage from animal husbandry farms].investigations revealed that under the conditions prevailing in the districts of sofia and .pernik ascaris suum and trichocephalus suis eggs remained viable at the conventional storage of liquid manure on the large farms for about a year, and fasciola hepatica and paramphistomum sp. eggs--for 2 to 3 months. the methods of electrocoagulation and electroflotation could be employed with success in the cleansing and rendering harmless of the sewage waters after the preliminary treatment with calcium ...1977919336
histochemical localization of alkaline phosphatase in the trematode paramphistomum cervi (paramphistomidae). 1976992820
trematodes of domestic ruminants of afghanistan and their role in pathology.seven trematode species were found in the bile ducts and rumen of domestic ruminants: fasciola gigantica, f. hepatica, gigantocotyle explanatum, g. siemense, paramphistomum epiclitum, fischoederius cobboldi and gastrothylax cruminifer. the parasites caused marked pathological changes in the liver and rumen.19761010490
studies on the stomach flukes of buffalo in egypt (trematoda: paramphistomata).three amphistomous species, paramphistomum gotio fukui, 1922; paramphistomum microbothrium fischoeder, 1901 and carmyerius gregarius (looss, 1896) have been found as a result of the investigation of stomach flukes of buffalo in egypt. specific status of paramphistomum gotoi, variability of paramphistomum microbothrium collected in different egyptian domestic ruminants and histological structure of the muscular organs of carmyerius gregarius have been examined.19761010492
[effectiveness of terenol-hoechst and zanil-ici in the control of paramphistomum sp. in cattle]. 19761017564
ultrastructural observations on the redial tegument of paramphistomum epiclitum from the planorbid snail, indoplanorbis exustus.the morphology of the tegument in the redia of paramphistomum epiclitum (digenea: paramphistomidae) resembles that shown by most larval and adult digeneans; an outer surface syncytium is in continuity with the cytoplasm of in-sunken, nucleated cytons. although tegumental cytons usually contain a single nucleus, some display up to six nuclei. the tegumental syncytium lining the pharynx of p. epiclitum rediae lack underlying cytons. the apical membrane of the tegument is elaborated by folds and mi ...19921452991
ultrastructure and histochemistry of the digestive tract of juvenile paramphistomum epiclitum (paramphistomidae: digenea) during migration in indian ruminants.the digestive tract of juvenile paramphistomum epiclitum consists of a foregut with a highly muscular terminal pharynx and an oesophagus, which leads to a pair of unbranched and blind-ending intestinal caeca. a syncytium lining the foregut is continuous with the external tegument and displays similar sensory papillae and secretory bodies (t1 and t2). a third type of secretory body (t3) is confined to the oesophageal cytons of newly excysted juveniles and is first evident in the syncytium by day ...19921487369
ultrastructure and histochemistry of the protonephridial system of juvenile paramphistomum epiclitum and fischoederius elongatus (paramphistomidae: digenea) during migration in indian ruminants.the protonephridial system of juvenile paramphistomum epiclitum and fischoederius elongatus consists of a bilaterally symmetrical arrangement of primary, secondary and tertiary ducts which connect individual flame cells with a simple common bladder. primary and secondary ducts are formed from columns of adjoining cells which provide an epithelial lining, whose luminal surface is elaborated with either short tubercles or lamellae. groups of cilia project from the luminal surface at frequent inter ...19921487370
ultrastructure and histochemistry of the lymph system and parenchyma of juvenile paramphistomes (paramphistomidae: digenea) during migration in indian ruminants.the lymph system of juvenile paramphistomum epiclitum and fischoederius elongatus consists of a single pair of longitudinal primary vessels from which sub-dividing branches extend laterally to associate with most major tissues and organs. the system originates shortly after excystation in the definitive host and is fully developed in day 14 juveniles. lymph vessels are syncytial and membrane limited, with a matrix which contains autophagic-like inclusions, clusters of ser and free nuclei. simila ...19921487371
comparison of the hemoglobins of the platyhelminths gastrothylax crumenifer and paramphistomum epiclitum (trematoda: paramphistomatidae).1. gastrothylax crumenifer and paramphistomum epiclitum parasitize the water buffalo bubalus bubalis. 2. gastrothylas hemoglobin consisted of two fractions of ca 30,000 and ca 18,000 by gel filtration. sds-electrophoresis showed both to be single, ca 15,000 chains. 3. paramphistomum hemoglobin was ca 16,000 by both gel filtration and sds-electrophoresis. 4. reversed-phase chromatography of carboxymethylated trematode and buffalo globins gave single peaks and two peaks, respectively. although par ...19921611885
use of landsat mss imagery and soil type in a geographic information system to assess site-specific risk of fascioliasis on red river basin farms in louisiana.a geographic information system (gis) was constructed in an erdas environment using maps of soil types from the usda soil conservation service, landsat satellite multispectral scanner data (mss), boundaries for 25 study farms, and slope and hydrologic features shown in a two-quadrangle (usgs, 7.5') area in the red river basin near alexandria, louisiana. fecal sedimentation examinations were done in the fall of 1989, spring of 1990, and fall and winter of 1990-1991 on 10-16 random samples per her ...19921626889
epidemiology of paramphistomosis in cattle.the epidemiology of paramphistomosis in cattle was studied using tracer calves in a subtropical location in eastern australia. two species of paramphistomes were present; calicophoron calicophorum and paramphistomum ichikawai. the former species was the most abundant. gyraulus scottianus and helicorbis australiensis acted as intermediate hosts, respectively. paramphistome burdens varied seasonally and were dependent upon the number of infected host snails. peak fluke burdens and clinical paramph ...19911774118
parasites of livestock in asir province, southwestern saudi arabia.thirteen parasite species, three trematodes, five cestodes, one nematode, and four arthropods, have been reported in livestock in asir, southwestern saudi arabia. the occurrence of dicrocoelium dendriticum in imported sheep and paramphistomum cervi in indigenous cattle is recorded for the first time in saudi arabia.19902267729
lipid biosynthesis in paramphistomum microbothrium (trematoda).in vitro studies with paramphistomum microbothrium indicated that the trematode is capable of synthesizing its complex lipids using exogenous substrates. u-14c glucose and 2-14c acetate were predominantly incorporated into phospholipids while 1-14c oleic acid and u-14c palmitic acid appeared more in the neutral lipids. a large proportion of the labelled acetic acid incorporated into neutral lipids appeared in the triglycerides. p. microbothrium is capable of synthesizing its cholesterol de novo.19902337254
the susceptibility of lymnaeid snails to paramphistomum cervi infections in mexico.the occurrence of paramphistomum cervi has been reported in mexico, but its intermediate hosts have not been identified. five species of snails of the genus lymnaea, l. bulimoides, l. columella, l. cubensis, l. humilis and l. palustris, were collected in mexico. only three of the exposed species, l. palustris, l. cubensis and l. humilis, became infected with p. cervi. the first of these snail species was highly susceptible to infection and a higher number of the exposed snails shed cercariae. it ...19902343525
[antigenic structure of dicrocoelium dendriticum (rudolphi, 1819)].the purpose of this paper was to study the antigenic structure of dicrocoelium dendriticum with a particular consideration of its antigenic relationship between f. hepatica and paramphistomum sp. studies of the antigenic composition of somatic extract of d. dendriticum and its four fractions obtained by gel separation chromatography on sephadex g 75 were carried out by using double diffusion reaction with homologous (sdd) and heterogeneous (sfh, sps) rabbit sera as well as sera of sheep naturall ...19872455289
helminth development stages isolated from cattle faeces and herbage in pastures of rural areas in imo state, nigeria.examination of faecal and herbal samples from pastures of 6 villages in various parts of imo state exhibited a preponderance of helminth eggs and infective larva. the percentage of the various genera present was assessed. these genera include: oesophagostomum, trichostrongylus, cooperia, strongyloides, neoascaris, haemonchus, trichuris (nematodes), fasciola, cotylophoron, paramphistomum (trematodes), moniezia (cestodes). the results of investigation showed that the abundance of these development ...19892610677
ultrastructure and cytochemistry of the tegument of orthocoelium scoliocoelium and paramphistomum cervi (trematoda: digenea).the tegument of orthocoelium scoliocoelium and paramphistomum cervi was examined using histochemical techniques and electron microscopy. on the basis of the distribution of acid and alkaline phosphatase (e.c. 3.1.3.2, e.c. 3.1.3.1), non-specific esterase (e.c. 3.1.1.1), cholinesterase (e.c. 3.1.1.7) and succinate dehydrogenase (e.c. 1.3.99.1) at light microscope level two distinct regions were recognized, an outer and an inner zone. electron microscopy revealed that the tegument comprises an out ...19882976778
[the first finding of paramphistomum daubneyi (dinnik, 1962) in beef cattle in algeria].the first finding of the causative agent of paramphistomatosis of cattle in the coastal region of algeria is described. as found on the basis of histological diagnosis, this causative agent is the species paramphistomum daubneyi (dinnik, 1962). lymnaea truncatula was found to be present as a potential intermediate host at the localities where the cattle harbouring these trematodes were kept. it is suggested by the high intensity of invasion by the trematodes (up to 2204 specimens in one host) th ...19873113035
[description and life cycle of paramphistomum microbothrium fischoeder, 1901, (trematoda, paramphistomidae) in algeria]. 19883268050
seasonal reproduction in paramphistomum epiclitum and gastrothylax crumenifer, rumen paramphistomes of the indian water buffalo, and comparison with the biliary paramphistome gigantocotyle explanatum. 19883417379
morphological and histochemical observations on the vitelline cells of developing and adult paramphistomum cervi (trematoda: digenea).in 6 week-old paramphistomum cervi, vitelline cells appear in the form of a few scattered cells, present below the body wall which divide mitotically to form vitelline follicles in 8 week-old worms. small shell globules appear first in 10 week-old worms, the number and size of which continue to increase till the worm becomes adult. in the latter the vitelline follicles contain stem cells, immature, maturing and mature vitelline cells, which differ due to the distribution of shell globules as wel ...19873437110
detection and quantitation of humoral immunity to paramphistomum microbothrium in gut mucosa of cattle. 19873453515
[superficial argentophilic structures in the miracidium and cercaria of paramphistomum microbothrioides price and mac intosh, 1944 (trematoda, paramphistomidae)].superficial argentophilic structures of the miracidium are identical to those observed in the other species of the genus. on the other hand, on the terebratorium, they are different from those of the miracidium of calicophoron calicophorum. cercarial papillae are rarely symmetrical and their number varies according to specimens. however differences between the species are obvious. if paramphistomum microbothrioides, p. microbothrium and p. daubneyi are related by acetabular papillae, the number ...19863566081
histochemical studies on eggshell formation in paramphistomum cervi (digenea: paramphistomatidae).the formation of the egg mass in the ootype, containing the oocyte, vitelline cells, numerous spermatozoa and mehlis' gland secretion marks the beginning of eggshell formation. mehlis' gland cells pour their lipoprotein secretion into the ootype; this secretion forms a thin template around the egg mass and upon this template the shell globules are deposited. eggshell formation begins in the proximal uterus and is completed in the middle uterus. the eggshells are devoid of phenols and phenolase i ...19873571922
on the metacercaria on paramphistomum (p.) microbothrium (trematoda, digenea): morphological, histological and histochemical study. 19873572069
[chaetotaxy of the cercaria of paramphistomum sp., a parasite of cattle in vendée].the cercaria was shed by an anisus rotundatus experimentally infected with a parasite of bos taurus in vendée (france). this trematode was identified as paramphistomum cervi. the snail natural host is unknown. the chaetotaxy of the cercaria differs from that of other european species of paramphistomum and particularly from p. leydeni and p. daubneyi both species which occur in vendée. the morphology of the adult precludes any relationship with p. ichikawai also present in vendée. therefore it ve ...19873662332
morphological and histochemical studies on the development of testis and spermatogenesis in the ruminal trematode of sheep, paramphistomum cervi (digenea: paramphistomatidae).morphological and histochemical changes accompanying testicular development and spermatogenesis have been described in the ruminal trematode paramphistomum cervi during the course of its infection in sheep. small testes of 4-week-old worms contain a few primordial germ cells. spermatogonial cells appear first in 6-week-old worms and increase in number by the 10th week when the testes become large and follicular. mature spermatozoa appear in the testes of 16-week-old worms. general pattern of spe ...19863721355
histochemical alterations in the duodenum of goats experimentally infected with paramphistomum cervi.certain histochemical alterations in the different tunics of duodenum in kids were studied 20, 40, 60 and 80 days post-infection (dpi) with paramphistomum cervi and the results compared with those of uninfected kids. there was a general reduction of polysaccharide complex substances and glycogen at 20 dpi. a marked decrease in polysaccharide complex substances and glycogen at 20 dpi. a marked decrease in polysaccharide complex substances and glycogen was especially discernible in the brunner's g ...19853992867
[pathogenesis of paramphistomiasis in sheep].experimental studies were carried out on the pathogenesis of paramphistomum infection of 58 weaned lambs, aged 4 to 12 months, infected with various numbers of adolescaria. the test animals were divided into 5 groups, each group having 2 subgroups. most of the animals were given the infectious material only once, while some of them were infected three times at 30-day intervals. the animals of the fifth group were infected with 5,000 live worms each. adolescaria were obtained from experimentally ...19853992928
[parasite egg development during methane fermentation].investigations were carried out on the development of the most frequently encountered parasitic diseases in farm animals raised under industrial conditions. the parasite eggs were obtained from great numbers of animals with fascioliasis, dicrocoeliasis, ascaridiasis, and paramphistomum infection, etc., and were mixed with fecal homogenate in a lagoon of a fermentor in the conditions of methane fermentation. it was found that the parasite eggs in such conditions retained their vitality in a state ...19854013079
on the species of paramphistomum of cattle and sheep in hungary. 19744420761
contribution to the morphology and distribution of paramphistomum microbothrium fischoeder, 1901 in cattle and sheep in the balkans. 19744452489
trials with rafoxanide. 5. efficacy studies against fasciola hepatica, fasciola gigantica, paramphistomum microbothrium and various nematodes in sheep. 19724670768
[intermediate hosts of paramphistomum sp]. 19725026996
anthelmintic efficacy of 2,6-dihydroxybenzoic acid-4'-bromanilide (hoe 296 v) against mature and immature paramphistomum microbothrium in goats. 19695381635
pathological lesions in livers of two indian sambars (cervus unicolor niger) infected with paramphistomum explanatum creplin, 1847; nasmark, 1937 gigantocotyle explanatum. 19695389383
geographic distribution of gongylonema pulchrum, gongylonema verrucosum, and paramphistomum liorchis in white-tailed deer of the southeastern united states. 19705461089
histochemical observations on the spermatogenesis of the trematode, paramphistomum (explanatum) bathycotyle. 19705487417
[intestinal paramphistomiasis in ruminants. experimental infection of sheep with metacercariae and immature forms of paramphistomum cervi (schrank, 1790)]. 19705530163
gametogenesis in paramphistomum microtothrium fischoeder, 1901. 19715569879
[on the biology of paramphistomum ichikawai fukui]. 19675618667
host-parasite relationships of paramphistomum microbothrium fischoeder, 1901, in experimentally infested ruminants, with particular reference to sheep. 19675626000
a study of the epidermal structures of the miracidia of calicophoron calicophorum (fischoeder, 1901) näsmark, 1937 and paramphistomum microbothrium (fischoeder, 1901). 19676051887
cytochemical studies on the prostate glands of the trematodes, paramphistomum epiclitum and paradistomoides orientalis.pear-shaped prostatic gland cells which surround the ejaculatory duct of paramphistomum and paradistomoides, secrete two types of products: (i) granules which are cytochemically complex containing lipids, proteins and polysaccharides (1:2, glycol groups) and (ii) globules which contain phospholipids only. the cytoplasm of these cells, in addition to high acid phosphatase activity, also contains rna, proteins and polysaccharides.19806164713
[superficial argentophilic structures of miracidium and cercaria of paramphistomum phillerouxi dinnik, 1961 (trematoda, paramphistomidae) (author's transl)].superficial argentophilic structures of the miracidium and cercaria of paramphistomum phillerouxi dinnik, 1961, are descrifed in detail. concerning the miracidium they are similar to those of the miracidia of some species belonging to the genus. on the other hand the chaetotaxy of the cercaria is different from that of the cercaria of p. togolense (alberet et coll., 1978) and therefore this appears to constitute a good criterium for the taxonomy of this group.19816167192
[argyrophilic integumentary structures of the miracidium, redia and cercaria of paramphistmun daubneyi dinnik, 1962 (trematoda, paramphistomidae)].paramphistomum microbothrium and p daubneyi are two sibling-species. the value of morphological criteria which have been used to distinguish the adults is not accepted by all authors. on the contrary, criteria based upon larval stages, except miracidia, permit an easy diagnosis. p. microbothrium parasitizes bulinids, the rediae have a long intestine, germ-balls are inequal in size, cercariae are long-tailed and possess numerous lateral somatic bristles: about 9 by a half-body and numerous caudal ...19816167193
[immunodiffusion studies of the complete somatic antigens of paramphistomum sp. and fasciola hepatica].investigations were carried out through the agar gel immunodiffusion test of complete somatic antigens of paramphistomum sp. and fasciola hepatica, using the respective homologic and heterologic hyperimmune rabbit sera. differences were established in the spectrum and number of the produced precipitation lines - 6 to paramphistomum and 4 to fasciola. with the identity test by the scheme in three after zilber and abelev (1962) no precipitation arcs were formed against the heterologic hyperimmune ...19816172897
morphological and histochemical studies on the ovarian development and oogenesis in paramphistomum cervi (digenea: paramphistomatidae).the ovary in 4-week-old worms contains undifferentiated germ cells. the oogonial differentiation, together with the appearance of some pas-positive material in the ooplasm occurs in the ovary of 8-week-old worms. the ovary of 16-week-old worms contains only oogonia and oocytes and no germ cells. in adult worms, the oocytes reach the pachytene stage and further development is arrested at this stage. nucleolar fragmentation and subsequent transport of nuclei to the ooplasm have been observed. the ...19846204914
study of phosphagens in two trematodes.extracts from the adults of two trematodes fasciola gigantica and paramphistomum explanatum have been shown to contain three phosphagens, phosphoarginine, phosphocreatine, and phosphoglycocyamine. the three phosphokinases involved in the transphosphorylation of these phosphagens in both worms were inhibited by edta and mercuric ions while iodoacetate had no effect.19806245542
[seasonal changes and incidence of paramphistomum sp. infection in cattle in southeastern poland]. 19846531928
histopathology of the duodenum and rumen of goats during experimental infections with paramphistomum cervi.on microscopic examination after experimental infection with paramphistomum cervi, tissue reactions in the duodenum were more pronounced during early stages of the infection (20th day post-infection (dpi)). immature parasites were seen migrating to the muscularis layer, and focal infiltration of macrophages and lymphocytes was observed in the lamina propria and in the interstitial tissue of brunner's gland. at places, there was cystic dilatation of brunner's gland. at 40 dpi, the parasite was no ...19846541393
maturation of paramphistomum cervi in sheep in india.histological studies of the trematode paramphistomum cervi collected throughout the year from the rumen of sheep, revealed the occurrence of gravid worms from april to august and immature worms from september to march. the maturation of the gonads and the accessory sex glands i.e., the prostate-, mehli's- and vitelline-gland began during the period march-april and showed functional peaks during the period july-august, which coincided with the availability of the intermediate host, indoplanorbis ...19846541837
the effects of environmental factors on the development, behaviour and survival of paramphistomum microbothrium miracidia.the effects of temperature, hydrogen ion concentration, salts, light, gravity and living organisms on the development, survival and behaviour of paramphistomum microbothrium miracidia were studied. isotonic water environment of ph 7-8 and temperature of 25 degrees c were found to be most favourable for miracidial development and hatching. miracidia longevity was highest at 21 degrees c in an isotonic water environment of ph 6-8. demineralized water reduced the longevity of miracidia. miracidia e ...19846543054
[biology of paramphistomum petrowi--a parasite of axis deer in the maritime territory].experiments have shown that developmental period of eggs of p. petrowi under humid conditions depends directly on temperature. at 30 c their development is over in 8, at 26-27 c in 14 and at 18.5 c in 40 days. under natural conditions at daily average temperature of 17.5 c the hatching of miracidia occurred in 52 days. intermediate host of this trematode in a zone of park deer breeding is a freshwater mollusc of the family planorbidae, anisus minusculus. the development of parthenites in the int ...19836622060
[argyrophilic structures of the larval forms of 2 strains of paramphistomum microbothrium: 1 a parasite of isidora guernei and ovis aries, the other a parasite of isidora truncata and bos taurus].the life cycle of a paramphistome from north senegal is completed starting from cercariae shed by naturally infected isidora guernei: a sheep and then uninfected isidora guernei were successively infected experimentally. adults obtained were identifiable as p. phillerouxi or as p. microbothrium. species determination cannot be made on the basis of argentophilic structures in the miracidium. morphology of sporocysts and rediae differs from that observed in p. phillerouxi but is undistinguishable ...19836660795
[what is paramphistomum cervi?].it is proved histologically that lacking of tegumented papillae in paramphistomum cervi is based on the alteration of the tegument furnished with papillae in the normal state. the differences in the dorsal extension of the vitellaria, in the histological structure of the genital opening complex and in other features, used by eduardo (1982) for the division of p. cervi into 3 species, are refuted as not existing. therefore the forms p. leydeni, p. scotiae and p. hiberniae remain well established ...19836666870
some helminth and arthropod parasites of the grey duiker, sylvicapra grimmia.sixteen grey duikers were culled on the farm riekert 's laager in the central transvaal at irregular intervals from may 1979-march 1981. one trematode species, 3 cestode species and 16 nematode species were recovered from these animals. of these the following are new helminth records for this antelope: cooperia hungi , cooperia neitzi , cooperia pectinata , trichostrongylus axei, trichostrongylus colubriformis, trichostrongylus falculatus , trichostrongylus instabilis , impalaia tuberculata , ne ...19836676685
[superficial argentophilic structures of miracidia and cercaria of paramphistomum leydeni nasmark 1937].the life cycle of paramphistomum leydeni näsmark, 1937, is carried out in the laboratory. adults were obtained from the rumen of a naturally infected bos taurus in czechoslovakia. laboratory-reared planorbis planorbis experimentally infected shed the first cercariae two months after exposure to miracidia. argentophilic structures in miracidia and cercariae are described. in miracidia, these structures are similar to those previously described from species of paramphistomum: p. togolense, p. phil ...19846721375
trematodiasis in turkey: comparative efficacy of triclabendazole and niclofolan against natural infections of fasciola hepatica and f. gigantica in sheep.a new fasciolicide, triclabendazole, tested at single oral dose rates of 5 and 10 mg/kg body-weight, proved to be highly effective (greater than 99%) against chronic field infections of fasciola hepatica and f. gigantica in sheep. the performance of the new drug compared favourably with niclofolan at the recommended dose. neither product was effective against dicrocoelium dendriticum and paramphistomum spp.19846747253
[the incidence of flukes of the genus paramphistomum in czechoslovakia].an effort was made to evaluate objectively all findings on the variability of fluke of the family paramphistomatidae and to give a clear survey on the species occurring in this country in order to be able to control it successfully in the case of widespread occurrence of paramphistomatosis or its intermediate hosts. the individual fluke species have specific intermediate hosts and a correct determination can make preventive measurements easier. three species of rumen fluke live parasitically in ...19826814041
effects of carbon dioxide, temperature, ph and the number of worms on the in vitro survival, histology and histochemistry of paramphistomum cervi (paramphistomatidae: digenea).survival of immature and mature stages of paramphistomum cervi, collected from the intestine and the rumen of sheep has been studied in in vitro using tyrode's, hank's, eagle's, stephenson's saline and hedon--fleig media. twenty-five ml quantities of carbon dioxide (co2)-rich hedon--fleig solution at ph 6.7, at 37 degrees c supported the maximum survival of 5 worms of both immature and mature flukes for periods of 9 and 10 days, respectively. alterations to the ph of the medium, the incubation t ...19826819682
failure of a bovine strain of paramphistomum microbothrium to survive in the rumen of sheep. 19836836173
role of oesophageal glands in the digestive physiology of two rumen amphistomes orthocoelium scoliocoelium and paramphistomum cervi.histochemical techniques have been used to study the chemical composition of the oesophageal gland secretions of orthocoelium scoliocoelium and paramphistomum cervi. results suggest that the secretions contain numerous enzymes, e.g., non-specific alkaline and acid phosphatases, atpase, tppase, esterase (cathepsin c like), beta-galactosidase, beta-glucuronidase and n-acetyl-beta-glucosaminidase. the role of these enzymes in the digestion of food in these amphistomes is discussed. on the basis of ...19836841957
morphological and histochemical observations on the oocapt and oviducal transport of oocytes in paramphistomum cervi (zeder, 1790) (digenea: trematoda).the oocapt of paramphistomum cervi has a narrow constriction followed by a wide lumen with a thickened wall and is surrounded by several unicellular flask-shaped gland cells opening into the lumen. the oocapt gland cells are filled with granules, the size and number of which vary in the body cells and its duct. histochemical studies have shown that the oocapt gland cells secrete mainly mucoproteins but a few lipoprotein and phospholipid granules have also been observed. the passage of the oocyte ...19836875252
seasonal incidence of helminths in sheep and goats in south mozambique.the seasonal fluctuations in faecal worm egg counts were studied at monthly intervals for over a year in 20 male sheep and goats on extensive grazing tracts at a farm near maputo, mozambique. larval differentiation from faecal cultures showed that haemonchus contortus and oesophagostomum columbianum were the most prevalent worm species. trichostrongylus colubriformis, cooperia spp., strongyloides papillosus, moniezia spp., paramphistomum spp. and trichuris spp. also occurred. after the onset of ...19826892176
bovine fascioliasis in nigeria--intercurrent parasitic and bacterial infections.the bacterial and parasitic infections associated with field cases of bovine fascioliasis due to fasciola gigantica were investigated; 47.6% of infected cattle had blood parasites, 61.9% had faecal-borne parasites and 85.8% had positive bacterial isolates in the bile in contrast to 13.3% with blood parasites, 71.1% with faecal-borne parasites and 28.9% positive for bile bacteria among uninfected cattle. trypanosoma spp., babesia, paramphistomum and schistosoma were the most commonly found parasi ...19827101464
the seasonal incidence of helminth parasites of cattle in the eastern transvaal lowveld.two helminth-free tracer calves were exposed to natural helminth infestation of a farm in the eastern transvaal lowveld for one month. on a nearby farm calves grazed with the herd for at least 3 months before slaughter so that the cumulative worm burden could be determined. the gastro-intestinal parasites increased markedly after rain. the major species and order or prevalence were: cooperia pectinata, cooperia punctata, haemonchus placei and oesophagostomum radiatum; minor species were bunostom ...19827175905
[study of the therapeutic efficacy of nilsan preparation against paramphistomatidae and nematode infections in sheep].experiments were carried out with the preparation nilsan (oxiclozanide + levamizal) in the treatment of sheep with fasciola, dictyocaulus, and trichostrongylus infection. the trials involved a total of 2782 sheep on two sheep-breeding farms, constituting 15 flocks. the liveweight of the adult animals ranged from 40 to 60 kg, and that of the year-old female ones--from 40 to 45 kg. the daily ration on the two farms did not differ essentially from that offered to the animals in the past 2-3 years. ...19827179728
[cattle-associated infections caused by balanorchis anastrophus fischoeder, 1901 and paramphistomum sp. (trematoda, paramphistomidae) in rio grande do sul, brazil]. 19827182861
[occurrence of paramphistomum in poland cattle]. 19827183010
[problems in the differential diagnosis of the species paramphistomum daubneyi dinnik 1962 and paramphistomum microbothrium fischoeder 190].the taxonomic meaning of the morphological features of helminths is of paramount importance for their differential diagnostics. however, they should not be conceived as absolute, especially wih regard to trematodes such as p. daubneyi and p. microbothrium, similar not only on the basis of their morphological features but also with regard to their ultimate host-receivers as well as their geographical spreading. it is pointed out that for the differentiation of the above two types one has to take ...19807210495
[presence in france of paramphistomum leydeni näsmark, 1937 (trematoda:paramphistomata) (author's transl)].the authors point out, for the first time, the presence of paramphistomum leydeni näsmark, 1937 in the rumen of cattle originating from loire-atlantique and camargue. the parasite is briefly described again and its systematic position is discussed.19807224534
studies on amphistomiasis in andhra pradesh (india) vi. immunization of lambs and kids with gamma irradiated metacercariae of cercariae indicae xxvi.the life span and the pathogenicity of amphistomes developing from cercariae indicae xxvi (paramphistomum epicilitum) were considerably reduced when the metacercariae were gamma-irradiated at 2 krad or 3 krad. the higher irradiation dose resulted in the complete absence of the flukes in infected animals. single vaccination of lambs and kids with 3000 metacercariae irradiated at 2 krad or 3 krad stimulated a significant degree of resistance against challenge with 5000 non-irradiated metacercariae ...19817229328
the effect of five fasciolicides on malate dehydrogenase activity and mortality of fasciola gigantica, fasciolopsis buski and paramphistomum explanatum.the effect of oxyclozanide, hexachlorophene, nitroxynil, rafoxanide and diamphenethide on malate dehydrogenase activity of homogenates of fasciola gigantica, fasciolopsis buski and paramphistomum explanatum was investigated. the ratio of oxaloacetate reduction to malate oxidation in homogenates of fasciola gigantica, fasciolopsis buski and p. explanatum was 4.5:1, 3.6:1 and 5.2:1 respectively. oxyclozanide and rafoxanide at 10(-3) m inhibited enzyme activity by 100% in homogenates from all three ...19817264272
stereocan observations on the surface topography of gastrothylax crumenifer (creplin, 1847) poirier, 1883 and paramphistomum epiclitum fischoeder, 1904 (trematoda: digenea).the critical-point-dried specimens of gastrothylax crumenifer (creplin, 1847) poirier, 1883 and paramphistomum epiclitum fischoeder, 1904, both recovered from the rumen of sheep, were studied by scanning electron microscopy at magnifications ranging from 10 to 10,000x to reveal the structural differences of the tegument between the two species. in g. crumenifer, both dorsal and ventral surfaces have tubercle-like tegumental elevations, devoid of spines. a row of prominent, regularly arranged, bu ...19817276508
[method for obtaining paramphistomum daubneyi, dinnik, 1962 metacercariae].miracidium ova were obtained from the feces of a ram that was experimentally infected with paramphistomum daubneyi. the fecal masses were extended in the respective amount of water and were passed through a standard sieve, 0.150 and 0.080. the ova were collected in the second sieve, and were rinsed several times after the method of successive sedimentation. they were cultured in a thermostat at 26 degrees c in the course of 12 days. test molluscs of the g. truncatula species (a laboratory strain ...19817303483
[development of paramphistomum cervi from miracidium to metacercaria]. 19817337647
paramphistomum microbothrioides in american bison and domestic beef cattle.intestinal paramphistomiosis was diagnosed in a bull and cow with severe diarrhea and weight loss, and subsequent investigation suggested that the infection was transmitted from american bison (bison bison). the rumen fluke, paramphistomum microbothrioides, was recovered from the rumen of 3 bison on the farm or origin. single operculate eggs of p microbothrioides measuring 130 x 69 micrometers were recovered from the feces of the bull, from 9 of 10 bison, and from 3 of 9 other beef cattle. snail ...19817341559
time-course analysis of antibody response by eitb and elisa before and after chemotherapy in sheep infected with fasciola gigantica.the time-course analysis of the antibody response to fascicola gigantica infection in sheep was studied by enzyme-linked immunosorbent assay (elisa) and enzyme-linked immunoelectrotransfer blot (eitb). sera from sheep experimentally infected with f. gigantica were reacted with excretory-secretory antigens of the worm before and after chemotherapy with oxyclozanide. in elisa, there was a significant increase in anti-fasciola antibody by 2 weeks after infection and there was a sharp decrease in an ...19957571329
structural and functional comparison of trematode haemoglobins.haemoglobins from three different species of trematodes (gastrothylax crumenifer, paramphistomum epiclitum and isoparorchis hypselobagri) and their respective hosts were digested with papain. peptides so produced were separated on 10-15% sds-pagge. the probability of coincidence of the peptides was analysed statistically. oxygen affinity curves of trematode haemoglobins were found to be a rectangular hyperbolic. the hill coefficient values of all the trematode haemoglobins were found to be 1. th ...19957622793
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