Publications

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[parasitic helminths of the cecum and colon of equidae in italy].intestinal helminths from coecum and colon were studied in 93 equidae including 40 horses, 36 donkeys and 17 mules. a total of 38 species, 36 nematodes and 2 cestodes, were identified as follows: 1) triodontophorus serratus, 2) triodontophorus brevicauda, 3) strongylus equinus, 4) strongylus edentatus, 5) strongylus vulgaris, 6) cyathostomum tetracanthum, 7) cyathostomum coronatum, 8) cyathostomum labiatum, 9) cyathostomum labratum, 10) cyathostomum alveatum, 11) cyathostomum pateratum, 12) cyat ...19921339978
prevalence and abundance of equine strongyles (nematoda: strongyloidea) in tropical australia.a postmortem survey of 57 horses in tropical northern queensland revealed 41 (89%) infected with intestinal strongyles. thirty-five strongyle species (8 large strongyles and 27 small strongyles [cyathostominae]) were recorded of which 9 species are reported from australia for the first time. the 14 most prevalent small strongyles were cyathostomum catinatum (in 76% of horses), cyathostomum coronatum (65%), cyathostomum pateratum (33%), cyathostomum labiatum (30%), cylicostephanus calicatus (70%) ...19902380857
the prevalence and intensity of internal parasites of horses in the u.s.a.fifty-five adult horses were necropsied over a 15-month period, and their worm burdens counted and speciated. twenty-one species of cyathostominae were recovered. ten species: cyathostomum catinatum, cylicocyclus nassatus, cylicostephanus longibursatus, cyathostomum coronatum, cylicostephanus goldi, c. calicatus, c. minutus, cylicocyclus leptostomus, c. insigne and cyathostomum pateratum, comprised 98.9% of the total cyathostome burdens. these same 10 species also demonstrated high prevalences i ...19846237483
allele-specific pcr for the beta-tubulin codon 200 ttc/tac polymorphism using single adult and larval small strongyle (cyathostominae) stages.it has been shown that benzimidazole (bz) resistance in sheep gastrointestinal nematodes is linked with an increase in beta-tubulin codon 200 tyrosine-expressing alleles in the resistant parasite populations. here, an allele-specific pcr has been developed for the discrimination of the tac/ttc polymorphism in the beta-tubulin 200 codon of small strongyles. one reverse primer was used in 2 separate amplifications with 1 of 2 forward primers that differed only in their final 3' nucleotide. the pri ...200212053994
investigation of diversity and isotypes of the beta-tubulin cdna in several small strongyle (cyathostominae) species.the diversity of the beta-tubulin cdnas of the cyathostominae and the occurrence of further isotypes were examined in adult worms isolated from an anthelmintic-naïve horse. cdnas encoding beta-tubulin from cyathostomum catinatum, cylicocyclus nassatus, cylicocyclus insigne, cylicocyclus radiatus, cylicocyclus elongatus, cyathostomum coronatum, and cyathostomum pateratum were characterized using specific primers developed from the cdna sequence of cc. nassatus. the cdna sequences span 1,429 bp an ...200212197112
beta-tubulin complementary dna sequence variations observed between cyathostomins from benzimidazole-susceptible and -resistant populations.the molecular mechanism of benzimidazole (bz) resistance in cyathostomins of horses is still unclear. previous studies revealed that the ttc or tac polymorphism in codon 200 of the beta-tubulin isotype 1 gene is not as strictly correlated with bz resistance as in trichostrongyles in sheep. to identify further sites of polymorphism within the beta-tubulin gene related to bz resistance, complete complementary dnas (cdnas) encoding beta-tubulin of adult worms of cylicocyclus nassatus, cyathostomum ...200415357086
evidence of p-glycoprotein sequence diversity in cyathostomins.p-glycoproteins (pgps) are adenosine triphosphate-binding transporter proteins thought to be associated with multi-drug resistance in mammals and protozoans and have been suggested to be involved in the mechanism of ivermectin (ivm) resistance in haemonchus contortus. until now, resistance to ivm has not been reported in cyathostomins in horses in spite of its widespread and frequent use. reasons for this might be differences in the molecular mechanism of the development of resistance. based on ...200415562598
morphometric identification of equid cyathostome (nematoda: cyathostominae) infective larvae.the prevalence of infection with cyathosthomes of horses is high worldwide. identification of species on infective larvae based on available morphological keys is not fully accurate and the aim of the present study was to provide a reliable identification key of cyathostomes infective larvae. at the abattoir, horse large intestines were examined and the cyathostomes females extracted and identified. the eggs from uteruses of identified cyathostomes females were used for breeding the infective la ...200919359100
distribution and species-specific occurrence of cyathostomins (nematoda, strongylida) in naturally infected horses from italy, united kingdom and germany.a broad scale study was carried out in 2008 to evaluate the distribution and species-specific occurrence of cyathostomin populations in horse yards from europe. in total 102 properties and 3123 horses were included in italy (60 yards and 1646 animals), united kingdom (22 yards and 737 animals) and germany (20 yards and 740 animals). individual faecal samples were examined with a mcmaster technique while pooled samples were subjected to the microscopic examination of in vitro cultured larvae and ...201019906489
field study on the efficacy of an oral 2% ivermectin formulation in horses.twenty horses naturally infected with nematodes were included in a blind, controlled field study on efficacy and safety of an oral 2% ivermectin formulation at a dose of 0.2 mg.kg(-1). horses were divided into treated and non-treated (control) groups with ten animals each based on preliminary counts of eggs per gram of feces (epg). stool samples were collected after treatment for identification of nematode species. clinical evaluations and epg counts were performed on days 0, +5, +14 and +19. ni ...201121722495
the prevalence, abundance and distribution of cyathostomins (small stongyles) in horses from western romania.forty seven working horses from romania were post-mortem examined for small strongyles (cyathostominae) infections. all horses were found infected. the overall cyathostomins intensity ranged from 390 to 13,010 and horses were infected by 8-24 species. the intensity was higher in ventral colon (1531) and dorsal colon (824), the lowest in the caecum (524). twenty four species were identified. cyathostomum catinatum, cylicocyclus insigne, and c. nassatus had 100% of prevalence. over 50% of horses w ...201627198801
development of a recombinant protein-based elisa for diagnosis of larval cyathostomin infection.cyathostomins are ubiquitous nematodes of horses. once ingested, they can spend a substantial time as encysted larvae in the intestinal wall. the larvae can comprise up to 90% of the total burden, with up to several million worms reported in individuals. these stages can emerge in large numbers to cause life-threatening colitis. direct methods for detection of encysted larval burdens in live horses do not exist. previously, two antigen complexes were identified as promising markers for infection ...201627174468
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