| [purification of salmonella abortus ovis h antigen by using immunoadsorption]. | oh-antiserum was first produced in rabbit immunization with salmonella abortus ovis cultures and was later used for obtaining an immunoadsorbent after the method of avrameas and thernynck. the raw (not purified) h-antigen produced after the method of mccoy et al., was purified by the immunoadsorbent and its activity and specificity were studied serologically by the reaction of ouchterlony. it was established that the antigen reacts with the anti-h-salmonella serum, but does not react with normal ... | 1979 | 43005 |
| [modifications by nematoda of the sensitivity of mice to salmonella abortus ovis administered orally or subcutaneously]. | mice inoculated 6 to 9 days previously with heligmosomoides polygyrus (=nematospiroides dubius) become more frequently infected than controls after an oral challenge with a constant dose of salmonella abortus ovis. but parasitism increases the ld 50 when the subcutaneous route of infection is used, probably by stimulating the immune system of the host. | 1979 | 118826 |
| experimental salmonella abortus ovis infection of normal or primo-infected cd1 mice. | cd1 mice were inoculated intravenously or subcutaneously with sublethal doses of salmonella abortus ovis. kinetic studies demonstrate the invasion and multiplication potentiality of these bacteria in normal mice. primo-infected mice challenged subcutaneously into a hind footpad manifest an increased capacity to withstand the dissemination of the challenge bacteria from the inoculated region and to control the bacterial population in the draining lymph node and in the spleen. | 1979 | 507636 |
| [inactivated saponin vaccine against salmonellal abortion in sheep]. | an inactivated saponine vaccine is prepared from five highly immunogenic salmonella abortus ovis strains, selected by means of a biological test on white rats. saline was used as a diluent of the vaccine, with the addition of 30 per cent glycerine, 0.012 per cent saponine and 0.1 per cent propiolactone. the optimum immunization dose of 5 cm3 is injected singly subcutaneously behind the elbow, two and a half months after impregnation. the vaccine is applied on infected farms before the disease oc ... | 1978 | 571645 |
| [allergen effect on the number of monocytes in guinea pigs]. | followed up are the changes in the number of monocytes under the effect of venal injection of an allergen from salmonella abortus ovis to guinea pigs, sensibilized by live and killed cultures of the same salmonella. after inoculation of healthy guinea pigs with a live culture, the number of monocytes doubles and remains at this level up to the 11th day after the infection. the killed culture of this salmonella does not possess a similar action. a venal injection of salmonella abortus ovis allerg ... | 1978 | 753035 |
| [transmission of a delayed allergic reaction to salmonella abortus-ovis via the blood plasma of gamma-irradiated guinea pigs]. | use was made of blood plasma taken from guinea pigs (sensibilized with a live culture of salmonella abortus ovis and then irradiated wiht 800 rad gamma-rays) to transmit the skin allergy reaction to normal, nonsensibilized guinea pigs. the allergy reaction was demonstrated in the recipients of plasma as early as the 3-4th hour following the injection of the allergen into the skin. it reached its peak at the 12-24th hour and later on strongly diminished, remaining in few of the animals only up to ... | 1977 | 906314 |
| [the course of a salmonella-abortus-ovis infection in a herd of sheep]. | | 1977 | 918642 |
| [effect of several biologically active substances on the reactivity and immune response of the body]. | experiments were carried out with a total of 140 guinea pigs to study the effect of a tissue emulsion of swine embryos on some of the general defense reactions, the cell and humoral immunity of the organism in the treatment with an intact and a killed (with 3 per cent formalin) vaccine produced with two strains of salmonella abortus ovis. investigated were the phagocytic activity of the lung macrophages and the immobile cells of the reticulo-endothelial system in the spleen and the liver, the pr ... | 1977 | 929970 |
| [induction of an allergic response of the delayed type against salmonella abortus-ovis]. | studied were several of the conditions of inducing an allergy reaction of a delayed type in guinea pigs experimentally infected with salmonella abortusovis as well as the correlation between the production of agglutinins and precipitins and the allergic reaction of a delayed type. it was found that a live culture of salmonella abortusovis produced strongest allergic effects when injected into the pads. the injection (in the pads) of a killed culture, using no adjuvant, and the oral administratio ... | 1977 | 929973 |
| [some properties of cytophil antibodies against the o-antigen of salmonella abortusovis]. | boyden's multilayer method was made use of to study the resistance to mercaptoetanol, and to temperature effects as well as the fixation capacity of macrophages of other animal species as regards cytophil antibodies from sera of guinea pigs immunized with a live culture of salmonella abortusovis. it was found that they are resistant to heating at 56 degrees c for 30 minutes, to mercaptoetanol, and are not fixed by murine macrophages. discussed is the biologic value of such type of antibodies. | 1975 | 1189281 |
| intraperitoneal infection with salmonella abortusovis is partially controlled by a gene closely linked with the ity gene. | the aim of the present study was to determine whether the ity gene, which controls the resistance to s. typhimurium infection in mice, also governs the resistance to s. abortusovis, a serotype specific for goat and sheep. during either i.v. or i.p. infection, balb/c mice (itys) were not able to control the growth of s. abortusovis and eventually died from infection. in contrast cba (ityr) or (c.cb)f1 (ityr/s) mice were able to control the growth of these bacteria. using congenic c.d2 ityr mice, ... | 1992 | 1544222 |
| prophylaxis of salmonella abortus ovis-induced abortion of sheep by a salmonella typhimurium live vaccine. | a salmonella typhimurium live vaccine with optimal level of attenuation for sheep, constructed by means of 'metabolic drift' mutations, was tested for its efficacy in preventing salmonella abortus ovis-induced abortions. in two field trials in kirgiziya, 78,000 to 100,000 first delivery sheep received a fully tolerated single dose of 10(9) c.f.u. live vaccine 2 months before to 4 months after insemination. alternatively, they were immunized twice with commercial inactivated s. abortus ovis vacci ... | 1992 | 1574918 |
| epidemiological evaluation by rrna-dna hybridization of strains of salmonella enterica subsp. enterica serovar abortusovis isolated in southern italy in the years 1981-1989. | salmonella enterica subsp. enterica serovar abortusovis is a major agent of abortion of the sheep and is firmly established, although at low prevalence, in sicily. this paper describes the application rdna gene restriction pattern fingerprinting to investigate relatedness among 7 serovar abortusovis strains isolated at the "istituto zooprofilattico sperimentale" of sicily and 29 isolates identified at the southern italy centre of enterobacteriaceae between 1981 and 1989. although abortusovis ser ... | 1991 | 1727022 |
| phenotypic analysis of splenic lymphocytes and immunohistochemical study of hepatic granulomas after a murine infection with salmonella abortusovis. | infection in mice with an attenuated strain of salmonella abortusovis (sao), a specific pathogen for sheep, was used as a convenient model to understand further the induced immunity against sao. the hypovirulent rv6 strain, subcutaneously inoculated in salmonella-susceptible balb/cby (itys) mice, colonized the spleen and the liver in less than 6 days post-infection (pi) to be cleared after day 28 pi. simultaneously, an increase in spleen cell numbers, splenomegaly and hepatic granulomatous lesio ... | 1991 | 1783422 |
| experimental ovine salmonellosis (salmonella abortusovis): pathogenesis and vaccination. | salmonella enterica subsp. enterica ser. abortusovis, a sheep-adapted serotype, causes a contagious disease. abortion is the major symptom and the main source of contamination. research on this ovine disease may aid farmers, but may also contribute to comparative biological knowledge. innate resistance partly controlled by the ity locus, increased resistance to reinfection and humoral and t-cell-mediated immunity were observations gained with a murine model. in ewes, abortion regularly occurs fo ... | 1990 | 2101486 |
| [frequency of abortion and seroprevalence of the principal diseases causing ovine infectious abortion in the area of rabat (morocco)]. | a survey was carried out on 23 sheep flocks to estimate the frequency of abortion as well as the prevalence of antibodies against abortive infections. during the visit of each farm, a questionnaire was completed with the collaboration of the owner and blood samples were collected from all aborted ewes and some of those with normal lambing. a rate of 7% abortion was reached in both aborted and normally lambed ewes. anti-chlamydia psittaci antibodies were the most frequently detected (14 flocks). ... | 1990 | 2288452 |
| vaccinal properties of salmonella abortusovis mutants for streptomycin: screening with an ovine model. | living attenuated vaccines may help control infection with salmonella enterica subspecies enterica ser abortusovis in sheep without constraints incompatible with husbandry. virulence and immunogenicity of 2 abortusovis streptomycin-dependent strains and 3 reverse mutants were compared in sheep, the virulent parental strain abortusovis 15/5 being used as reference. reduction of virulence with conservation of some immunogenicity was demonstrated for the 5 mutant strains after intravenous or subcut ... | 1990 | 2344139 |
| [the isolation and characteristics of the biological and genetic properties of neamin-resistant mutants of salmonella abortus-ovis that are promising for vaccine creation]. | neamine-resistant mutants were obtained from s. abortus ovis virulent strain. these mutants were divided into three classes according to their sensitivity to streptomycin: mutants completely retaining their sensitivity, mutants sensitive to moderate and high doses of the antibiotic. on the basis of genetic analysis carried out with the use of bacteriophage p22, the near mutation of class near 100 strr 500 mutants was identified as nea b, and the near mutation of class near 100 strs, as nea a. th ... | 1990 | 2385985 |
| [the most frequent infectious causes of abortion in sheep in north bavaria with special reference to chlamydia and salmonella infections]. | between 1980 and 1987 1153 ovine fetuses and placentas were examined after abortion. in 68.5% of the cases a cause of abortion could be diagnosed as follows: 43.5% chlamydia psittaci var. ovis, 10.7% salmonella abortus ovis, 3.7% coxiella burnetii, 3.3% listeria monocytogenes and 0.4% campylobacter fetus intestinalis. hemolyzing escherichia coli and streptococci, yersinia pseudotuberculosis, salmonella typhimurium, salmonella enteritidis, corynebacterium pyogenes and other facultative pathogens ... | 1989 | 2503908 |
| [the differential diagnosis of salmonella abortus ovis and yersinia pseudotuberculosis in sheep abortions]. | 55 strains of salmonella abortus ovis and 3 strains of yersinia pseudotuberculosis (two serotypes i, one serotype ii), isolated out of aborted ovine fetuses from northern bavaria, were investigated and compared culturally, biochemically and serologically. the identification of s. abortus ovis (group b) could not be achieved by use of api 20 e. the code-number 4004500/47 identified s. typhi (group d) only. y. pseudotuberculosis serotype ii but not serotype i agglutinated with the polyvalent and t ... | 1989 | 2683516 |
| [ovine salmonellosis caused by salmonella abortus ovis]. | salmonella enteritidis subsp. enteritidis ser. abortus ovis, a sheep-adapted serotype, causes a contagious infectious disease with abortion as the main symptom, sometimes accompanied with mortality of lambs. this auxotrophic gram-negative bacterium ranks among the main causes of ovine abortions in some countries of europe and of western asia. the disease tends toward an endemic pattern with a multi-annual rhythm in the frequency of abortions. bacteremia leads to bacterial colonization of the fet ... | 1988 | 3069037 |
| anti-chlamydial immunity in ewes conferred by vaccination with a combination of three live chlamydia, brucella and salmonella vaccines. | live attenuated vaccines against chlamydia psittaci var ovis, brucella melitensis and salmonella abortus ovis have previously been shown to be compatible in mice by subcutaneous administration. immunity against challenge with virulent chlamydia was, however, slightly decreased in associations including the b melitensis rev 1 vaccine. the chlamydia strain 1b vaccine was administered to four- to five-month-old female lambs, either alone or in combination with the b melitensis rev1 and the s abortu ... | 1988 | 3176252 |
| [the epizootic process in ewe lambs experimentally infected with salmonella abortus ovis and chlamydia psittaci var. ovis]. | studied was the dynamics of antibody production and salmonella shedding in a flock of year-old female sheep affected with a mixed form of infection of salmonella abortus ovis and chlamydia psittaci var. ovis. it was established that in the presence of a mixed infection dominating was the part played by chlamydia organisms. the apparent epizootic process with these agents usually ran its course in the later period of pregnancy, while salmonella organisms acted by the end of the first half of preg ... | 1987 | 3439023 |
| [resistance of salmonella abortus ovis to disinfectant agents]. | by the method "test-vector" the influence of solutions of chlorinated lime, chloramine, sodium base formalin was investigated over 30 strains salm. abortus ovis isolated from fecal and vaginal samples, as well as from aborted foetus. it was established that s. abortus ovis manifests different stability against the separated disinfectants. these microorganisms are most sensitive to the action of chlorine preparations. for that reason is recommended to use the chlorinated lime and the chloramine w ... | 1987 | 3445449 |
| kinetics of experimental salmonella abortus ovis infection in ewes. | the multiplication and dissemination of salmonella abortus ovis, a salmonella serotype which is specific for small ruminants, were studied in 17 culled non pregnant ewes. the virulent strain 15/5, that has previously been used either for the development of mouse models or as an abortion inducing agent in sheep, was injected by the subcutaneous route at a sublethal dose of 10(9) salmonella per animal. in most animals, it induced only transient hyperthermia and pronounced local reactions. groups o ... | 1987 | 3451684 |
| [experimental infection with salmonella abortus ovis in rams]. | the susceptibility of rams to experimental challenge with salmonella abortus ovis was investigated by subcutaneous, conjunctival or preputial administration of 1 x 10(10) viable salmonella to 3 groups of 6 adult préalpes rams. slaughter and autopsy of 15 rams were made 83 days after challenge. each of the 3 remaining rams was introduced in 3 groups of 6 salmonellosis-free ewes. the subcutaneous injection caused a significant hyperthermia, a rapid increase in antibody titers without detectable ge ... | 1986 | 3548560 |
| simultaneous vaccination by three living attenuated strains of brucella, salmonella and chlamydia in mice. | associations of vaccines for simultaneous administration were successfully developed long ago, in particular in human medicine, although there have been very few attempts to associate living bacterial vaccines. unfortunately, living vaccines can interact with one another resulting in either a virulent infection or an exclusion of one by the other. having developed two new low virulence vaccinal strains of chlamydia psittaci var. ovis and salmonella abortus ovis for use in veterinary medicine, we ... | 1987 | 3577354 |
| [epizootiological evaluation of the peroral and vaginal infection of sheep with s. abortus ovis]. | epizootiologic evaluation is made of the oral and the vaginal salmonella abortus ovis infection in sheep in view of the occurrence and persistence of the infection within the sheep flocks. it has been established that the presence of carriers of infection depends on the physiologic status of the animals at the time of contracting the infection. abortion, lambing, and the setting in of estrus were shown to affect favourably the existing carrier status. salmonella abortus ovis organisms were most ... | 1987 | 3629948 |
| [diagnostic value of serological and bacteriological methods in sheep affected with s. abortus-ovis and chlamydia psittaci var. ovis infections]. | it was found that the shedding of salmonella organisms by sheep with salmonella abortions was most active during the first fifteen days following abortions or lambing. in sheep affected with a mixed salmonella abortus ovis and chlamydia psittaci var. ovis infection this period was longer (about 30 days). the excretion of salmonella organisms was more strongly manifested with the vaginal discharge than with the feces, urine, and milk. no correlation was established between the titer value of the ... | 1986 | 3727383 |
| [an attempt to create a factorial model of the epizootic process in sheep infected with s. abortus ovis]. | experiments were carried out to build up a factorial model with a group of sheep at different age in an infected region. studied were the aspects of the disease as caused by salmonella abortus ovis alone and in combination with chl. ps. var. ovis. it was found that the factorial model can explain sufficiently well (77.22 per cent) the discussed system--the epizootic process with year-old females and sheep affected with pure and mixed infection. the epizootic process with a mixed infection in the ... | 1986 | 3811206 |
| [intensity of the epizootic process in sheep infected with s. abortus ovis and chlamydia psittaci var. ovis]. | an attempt was made to analyze comparatively the intensity of the epizootic process in young female sheep, ewes (second pregnancy, with no records of abortions in their first pregnancy) and their lambs either with an infection of salmonella abortus ovis only or with a mixed infection of s. abortus ovis and chlamydia psittaci var. ovis. this was reached through following up and studying the parameters morbidity, mortality, lethality, index of infectedness, index of deadlines, and fertility. it wa ... | 1986 | 3811213 |
| [role of rams in the epizootiology of salmonellal abortion in sheep]. | studied was the susceptibility of rams to a salmonella abortus ovis infection. it was found that the animals could resist the oral experimental infection when the causative agent was introduced in the form of 20 ml broth culture or when the environment was contaminated with it. the salmonella abortus ovis infection was found to be transmitted from infected rams to normal ewes and vice versa, chiefly through sexual route. | 1986 | 3811223 |
| [salmonella abortus ovis carrier state in dogs and rats]. | serologic and bacteriologic investigations were carried out with 10 dogs, guarding sheep flocks with and without salmonella abortions, and 17 grey rats. it was found that both species of animals could harbour salmonella abortus ovis provided they swallowed infective material (aborted fetuses and fetal membranes). it was shown that although lasting but 2 to 3 months only the carrier state could play a definite part in the epizootiology of salmonella abortions in sheep. | 1985 | 3992911 |
| [the bactericidal titre, the level of properdin, gamma globulins and agglutinins in the blood of sheep infected with salmonella abortus ovis]. | | 1974 | 4139802 |
| [persistence of salmonella abortus ovis in soil]. | | 1974 | 4474679 |
| [oxygen consumption and dehydrogenase activity of salmonella abortus ovis strains with different lipopolysaccharide levels]. | | 1973 | 4799564 |
| [study of the dependence of oxygen consumption on the lipopolysaccharide-protein complex content in the antigenic structure of salmonella abortus ovis]. | | 1973 | 4802754 |
| isolation of salmonella abortus ovis bacteriophage and the determination of its specificity. | | 1969 | 5394124 |
| [on microbiology and mechanism of bacterial abortion. ii. experimental abortions with salmonella abortus ovis]. | | 1967 | 5601507 |
| titration of salmonella abortus-ovis strains with chick embrios and white mice. | | 1968 | 5698602 |
| endotoxic preparations from salmonella abortus-ovis and their toxicity in pregnant guinea pigs. | | 1968 | 5723993 |
| in-vitro sensitivity of salmonella abortus-ovis strains isolated from different parts of bulgaria to various antibiotics and chemotherapeutic agents. | | 1968 | 5752785 |
| clinico-microbiological and immunological studies on experimental oral infection in pregnant sheep with salmonella abortus ovis. | | 1969 | 5813685 |
| infection of sheep in yorkshire with salmonella abortus ovis. | | 1969 | 5815883 |
| [macrophage "disappearance" from the peritoneal cavity as affected by a specific antigen]. | investigations were carried out into the effect of a specific antigen on the changes in the macrophage count in the peritoneal exudate of guinea pigs sensibilized with a live salmonella abortus ovis culture, with and without an allergic reaction of a delayed type. the setting in of the allergic response was shown to correlate with the decrease in the number of macrophages--in animals with a positive response there was a strong drop of the macrophage count in the peritoneal exudate. it is stated ... | 1981 | 6179287 |
| [effect of levamisole on the formation of agglutinins against salmonellosis in weaned lambs]. | in order to follow up the effect of levamizol on the titer of agglutinins a killed vaccine of salmonella abortus ovis; s. enteritidis, and s. typhimurium strains was used under protection of antidenaturating agents (80 per cent sac charose) and levamizol ici at the rate of 10 mg per kg body mass. experiments were carried out with a total of 20 rabbits and 36 weaned lambs, divided into groups and injected with vaccine, vaccine and levamizol simultaneously, vaccine with levamizol following on the ... | 1984 | 6390935 |
| [experimental infection of sheep with salmonella abortus ovis: effect of the gestational stage]. | an inoculum of 1 x 10(10) s. abortus ovis was administered subcutaneously to non-pregnant pre-alpine x merino ewes (group 1 : 37 days before fecundation) and to pregnant ewes (groups 2 and 3: 33 or 89 days after fecundation). the proportion of abortions to gestations in each group was 0/9, 1/9, 8/10 respectively. in group 2, there was only one abortion which occurred 64 days after inoculation. in group 3, the average time between inoculation and abortion was 19 days; three ewes died of an s. abo ... | 1984 | 6486685 |
| [trial, in a contaminated environment, of an attenuated lyophilized vaccine against s. abortus ovis abortion in sheep]. | merinos ewe-lambs from arles formed part of a transhumant flock in which s. abortus ovis infection was enzootic. a lyophilized living vaccine was prepared from an attenuated mutant strain of salmonella abortus ovis. about one third out of 450 ewe-lambs of the same flock received one subcutaneous injection of vaccine 1 to 3 months before fecundation; the other ewe-lambs were used as controls. no modification of behaviour was observed after vaccination. three months later, serological post-vaccina ... | 1984 | 6517478 |
| [effect of routes and doses of salmonella abortus ovis inoculation on the abortive effect and serologic response of ewes]. | three successive experiments using a restricted number of préalpes x lacaune ewes were designed to form a procedure allowing the reproduction of an abortive infection with salmonella abortus ovis. effects of inoculations at mid-gestation were estimated by the duration of gestation, bacteriological excretion at lambing and evolutions of rectal temperatures and of serological antibodies. serological responses were revealed with four tests: o or h agglutination tests in tubes, h agglutination or co ... | 1983 | 6614790 |
| immunogenicity of a low-virulence vaccinal strain against salmonella abortus-ovis infection in mice. | strain rv6, a streptomycin-independent reverse mutant of salmonella abortus-ovis, multiplied and disseminated in vivo to a lesser extent than the virulent parental strain 15/5, as determined by subcutaneous inoculation of two outbred strains of mice with different susceptibilities to salmonella infection. this lower virulence of strain rv6 was not modified by 10 passages in susceptible mice. vaccination with strain rv6 induced a dose-dependent capacity of mice to control subsequent infection wit ... | 1983 | 6840854 |
| [examination of 112 strains of salmonella abortus ovis (author's transl)]. | | 1980 | 7009052 |
| [active immunoprophylaxis of salmonella abortion in sheep]. | a live vaccine was produced against the salmonellosis abortion in sheep with the use of an attenuated (chemically) strain of salmonella abortus ovis. it was tested comparatively with a killed vaccine in sheep under experimental conditions. the dynamics of the agglutinating antibodies was followed up after immunization with the two vaccines, studying also the class of the immunoglobulins. it was found that the production of class igg antibodies in sheep treated with the live vaccine proceeded mor ... | 1982 | 7157660 |
| [chemical and physicochemical properties of the h antigen (flagellin) isolated from salmonella abortus ovis]. | some chemical and physiochemical properties of h-antigen, obtained through immunoabsorption, were studied. by applying the method of davis, during a disc electrophoresis in a polyacrylic amide gel, it was proved that the molecule of flagellin is homogenous and is built up of only one polypeptic chain. on the other hand, by applying weber's and osborn's method, it was proved that flagellin is a protein having a molecular weight of about 53000 daltons. it showed its isoelectric point at ph 5.2, pr ... | 1980 | 7233827 |
| causes of abortion in khorassan province--mashad. | the aim of this paper is to analyse the cause of abortion and postnatal deaths in sheep on the basis of laboratory examinations. for serological examination against brucellosis and salmonellosis 899 blood samples were collected. from all tested sera 411 (45.7%) were positive to brucella melitensis antigen and only 10 (1.11%) to salmonella abortus ovis antigens. bacteriological examination were done with 50 lambs, 143 aborted foetuses and 46 vaginal swabs. brucella melitensis was stated in 7.6% i ... | 1981 | 7301632 |
| vaccinal properties of salmonella abortus ovis mutants for streptomycin: screening with a murine model. | streptomycin-dependent and streptomycin-independent reverse mutants of salmonella abortus ovis were selected on medium with and without added streptomycin. their in vivo properties were tested with a murine model. reduced capacities of dissemination and multiplication of all mutant strains were evidenced by means of subcutaneous, intraperitoneal, and intravenous inoculations of two outbred strains of mice with different susceptibilities to experimental s. abortus ovis infection. a factorial immu ... | 1981 | 7309235 |
| [mechanism of glycogen depletion in the liver in endotoxin-treated rats]. | studied were the rate and mechanism of glycogen breakdown in rats treated with salmonella abortus ovis endotoxin. it was found that tissue homogenates from treated animals showed higher glycogenolytic activity in vitro as compared with homogenates from untreated animals, which demonstrated the direct mechanism of endotoxin action. the higher lytic activity with treated animals seemed to be associated with the higher activity of glycogen-phosphorylase, which showed peak values at the third hour f ... | 1981 | 7340109 |
| coccidia parasitism increases resistance of mice to subcutaneous inoculation with salmonella abortus ovis. | resistance of outbred mice to subcutaneous challenge with s. abortus ovis was increased when previously or simultaneously parasitized with eimeria falciformis. this was measured by mortality rates and levels of splenic infection induced by salmonella in parasitized and control nmri or cd-1 (cr) mice. no effect was observed when e. falciformis was inoculated after s. abortus ovis. | 1981 | 7342834 |
| [characteristics of a salmonella abortus ovis allergen]. | studies were carried out to obtain an allergen of salmonella abortus ovis and establish its chemical composition and diagnostic value with guinea pigs and sheep infected with the same causative agent. it was found that the allergen produced contained a great amount of protein (75 per cent), an essentially low amount of sugars (11 per cent), and a negligible amount of total phosphorus (0.85 per cent). it was also established that the allergen did not induce the production of antibodies in rabbits ... | 1981 | 7344287 |
| experimental salmonella abortus ovis infection in ewes. | | 1980 | 7434498 |
| subcutaneous and conjunctival vaccination with a live attenuated strain of salmonella abortusovis: effect of gestation on serological response of ewes. | annual serological testing of flocks vaccinated by the subcutaneous route with a live, attenuated strain of salmonella abortusovis has previously demonstrated the persistence of agglutinating antibodies. it has however been impossible to determine whether the antibodies originated from the vaccination or from an enzootic infection. the serological response, as measured by a microtechnique of seroagglutination using a stained antigen, was studied in an isolated flock of 30 adult ewes. the trial p ... | 1995 | 7735301 |
| salmonella abortusovis experimental infection induced by the conjunctival route: clinical, serological and bacteriological study of the dose effect in female lambs. | the aim of this work was to explore the first stages of infection with salmonella abortusovis after a mucosal inoculation, and to establish an experimental model limiting the bacteriological investigations to the inoculation site. four groups of 6 ewe-lambs were infected by the conjunctival route with decreasing doses of salmonella abortusovis. the clinical and serological survey was completed by a daily bacteriological examination of nasal swabs and faeces. enumeration of viable bacteria was pe ... | 1995 | 7735306 |
| humoral immune response to salmonella abortusovis in sheep: in vitro induction of an antibody synthesis from either sensitized or unprimed lymph node cells. | in vitro culture conditions were determined to induce an anti-salmonella abortusovis antibody synthesis from lymph node leucocytes of three immunized sheep and two unprimed lambs maintained in culture in the presence of heat-inactivated bacteria for 2 weeks. humoral immune responses were assessed by enumerating specific antibody-secreting cells using elisaspot and by titrating immunoglobulins secreted into culture supernatants using elisa techniques. optimal secondary antibody response was obser ... | 1994 | 7941308 |
| resistance of nod mice to salmonellosis. | the nod mouse strain has become one of the most popular animal models for exploring insulin dependent type 1 diabetes. genetic studies have underlined the polygenic nature of the murine disease. two such genes were mapped on chromosome 1. one, associated with diabetes onset, is strongly linked with the lsh, ity, bcg genes encoding for resistance against l. donovani, s. typhimurium and mycobacterium. we have undertaken to phenotype nod mice with regard to one of these resistance genes. after infe ... | 1993 | 8044701 |
| comparative histopathology of draining lymph node after infection with virulent or attenuated strains of salmonella abortusovis in lambs. | histological responses to the early phase of infection were compared in parotid lymph nodes of lambs infected by the subcutaneous route into the right eyelid with either a virulent or an attenuated strain of salmonella abortusovis. the right parotid lymph nodes showed a massive pmn infiltration for the first days of infection for both strains. from day 6, the infected lymph nodes developed a lymphoid hyperplasia with prominent germinal centers independent of strain type. the virulent strain of s ... | 1994 | 8203128 |
| salmonella abortusovis infection in susceptible balb/cby mice: importance of lyt-2+ and l3t4+ t cells in acquired immunity and granuloma formation. | the role of t cells in granulomatous responses and in acquired immunity against salmonella abortusovis (sao) infection was studied in a murine model. mice were subcutaneously (s.c.) vaccinated with a live attenuated strain of sao. one month after vaccination, the transfer of primed spleen cells (1 x 10(8) cells per mouse) to syngeneic recipient mice conferred a significant protection of 3 log10, measured by spleen colonization on day 6 after s.c. challenge. in vitro treatment of spleen cells, be ... | 1993 | 8321117 |
| tnphoa salmonella abortusovis mutants unable to adhere to epithelial cells and with reduced virulence in mice. | salmonella abortusovis is a pathogenic bacterium highly specific to sheep, causing spontaneous abortion. in order to understand the role of genes involved in pathogenicity, we investigated s. abortusovis with the random mutagenic tnphoa transposon. a total of 95 s. abortusovis tnphoa mutants yielding alkaline phosphatase active fusion protein were obtained. in this way we created a bank of strains in order to identify any phenotypic modification which could affect the periplasmic and/or exported ... | 1993 | 8386703 |
| strain typing with is200 fingerprints in salmonella abortusovis. | a collection of salmonella abortusovis isolates was examined for the presence of insertion element is200. all proved to contain three or four copies of the element. one is200 hybridization band of approximately 9 kb was found in all isolates, indicating that all s. abortusovis strains carry an is200 element in similar or identical locations; this band can be potentially useful for serovar identification. s. abortusovis collection isolates from distinct geographic areas were highly polymorphic, s ... | 1996 | 8779575 |
| flow cytometric analysis of the reactivity of workshop monoclonal antibodies on sheep lymph node cells, after infection with salmonella abortusovis. | of the 302 monoclonal antibodies included in the third workshop on ruminant leukocyte antigens, 167 have been tested for their reactivity on uninfected and salmonella-infected sheep lymph node leukocytes using facs analysis. only 47 of them showed specificities which could be related to those of some control monoclonal antibodies, such as the anti-cd5, anti-ig light chain and anti-mhc class ii monoclonal antibodies. | 1996 | 8896231 |
| conserved structure of is200 elements in salmonella. | sequence analysis of three is200 elements (two from salmonella typhimurium, one from salmonella abortusovis) reveals a highly conserved structure, with a length of 707-708 bp and absence of terminal repeats. is200 contains an open-reading-frame (orf) which potentially encodes a peptide of 151 amino acids, with a putative ribosome-binding-site properly placed upstream of the orf. a potential rna stem-loop structure that might occlude the ribosome-binding-site of the orf is also found. another con ... | 1997 | 9060429 |
| [significance of causes of infectious abortion in sheep flocks in northern baden-württemberg with special reference to chlamydia psittaci]. | investigations on the reasons of infectious abortion cases in sheep flocks in northern parts of baden-wuerttemberg eludicate the wide-spreading of chlamydia psittaci (c. psittaci) and its significance as the most frequent cause of abortions in sheep. another important pathogen agent causing abortions is salmonella abortus ovis (s. abortus ovis) which could be demonstrated by using a serological elisa test. a less important role than c. psittaci and s. abortus ovis plays coxiella burnetii in abor ... | 1997 | 9092464 |
| in vitro cellular responses from sheep draining lymph node cells after subcutaneous inoculation with salmonella abortusovis. | salmonella abortusovis infection leads to ovine abortion. the basis for immunity against this infection is unknown. immune responses were studied from prescapular lymph node (psln) cells of sheep infected with either a subcutaneous inoculation of virulent (15/5) or a vaccine (rv6) strain and compared with those of uninfected sheep. psln cell phenotypes were characterized by immunofluorescence staining associated with flow cytometry. the in vitro responses were analysed using the psln cell prolif ... | 1997 | 9112738 |
| identification of salmonella abortusovis by pcr amplification of a serovar-specific is200 element. | field and collection isolates of salmonella abortusovis carry one is200 element in a distinct chromosome location. is200 is not found in the corresponding region of the chromosome of other salmonella serovars. sequencing of the boundaries of the s. abortusovis-specific is200 insertion permitted the design of primers for the amplification of this is200 element by pcr. isolates of s. abortusovis are identified by the amplification of a dna fragment of about 900 bp or larger. pcr amplification of d ... | 1997 | 9143137 |
| [detection methods for salmonella abortus ovis and examinations in sheep flocks in northern baden-württemberg]. | investigations into the incidence of salmonella abortus ovis (s. abortus ovis) infections should not be neglected in diagnosis of ovine abortion cases. for detection of this pathogen agent, direct cultivation, as well as pre-enrichment combined with enrichment procedures in tetrathionate or rappaport-vassiliadis medium, should be performed with respect to the features of s. abortus ovis. the detection limit of s. abortus ovis using pre-enrichment and enrichment media could be determined using 6. ... | 1997 | 9190780 |
| radioactive technetium-99m labelling of salmonella abortusovis for the assessment of bacterial dissemination in sheep by in vivo imaging. | we report the development and validation of a 99mtc-labelling technique of bacteria, applied to salmonella abortusovis. the radioactive labelling is obtained using a pre-tinning step of the cells followed by direct incubation of s. abortusovis suspension with 99mtc-pertechnetate. several procedures with different amounts of stannous tin (snf2 or sncl2) were evaluated. the selected method, respectful of bacterial viability, provided a 30% labelling yield. viability of 99mtc-labelled bacteria was ... | 1997 | 9355252 |
| mouse susceptibility to infection by the salmonella abortusovis vaccine strain rv6 is controlled by the ity/nramp 1 gene and influences the antibody but not the complement responses. | early growth of salmonella typhimurium in spleen and liver of mice is controlled by the mouse chromosome 1 locus ity/nramp 1. genetic control of resistance to the attenuated vaccine strain rv6 of salmonella abortusovis was studied in mice infected by the intravenous route. comparison of kinetics of bacterial colonization of spleen and liver in two congenic balb/c-susceptible (itys) and -resistant (ityr) mouse lines showed that balb/c mice (itys) were significantly more susceptible to infection t ... | 1998 | 9466946 |
| analysis of the immune response in sheep efferent lymph during salmonella abortusovis infection. | the efferent lymph duct of the ovine prescapular lymph node was cannulated, and salmonella abortusovis (sao), a specific pathogen for sheep inducing abortion and mortality of newborn lambs, was inoculated by the subcutaneous route in this lymph node drained area. while the prescapular lymph node draining the inoculation site represented an efficient barrier for the vaccinal sao rv6 strain spreading, sao 15/5 virulent bacteria were steadily detected in efferent lymph of infected sheep. the inocul ... | 1997 | 9533271 |
| salmonella abortusovis, strain rv6, a new vaccinal vehicle for small ruminants. | salmonella abortusovis strain rv6 (sao rv6) is a live attenuated vaccine used for a few years to protect ewes against abortive salmonellosis. as salmonellae, particularly salmonella aro mutants, have considerable potential as vehicles for the presentation of heterologous vaccine antigens, sao rv6 was tested in order to develop a vaccinal vehicle for small ruminants. five vector plasmids were tested in sao rv6; these plasmids, which carry maltose binding protein (mbp) expressed as protein, but di ... | 1998 | 9631532 |
| preparation of a high titre salmonella abortusovis h-antigen and antiserum and improvement of the microagglutination test. | serological diagnosis of small ruminant abortions due to salmonella abortusovis (s.a.o) was improved by using modified procedures to produce high titre h-antigens and anti-h serum. while the titres of the two standard antigens were 1:8 and 1:20, both modified antigens had a titre of 1:50. similarly, the use of adjuvanted and non adjuvanted antigens for the production of anti-h hyperimmune serum resulted in a titre of 1:160,000 as compared to 1:20,000 when standard procedures were used. in additi ... | 1999 | 10333073 |
| a salmonella abortusovis inactivated vaccine protects mice from abortion after challenge. | two types of salmonella abortusovis vaccines were prepared, one with aluminium hydroxide (vaccine a) and the other with water in oil (vaccine b) adjuvants. they were compared in a pregnant mouse model, aiming at protecting them from abortions after challenge with a virulent strain of s. abortusovis. the protection for vaccine a was from 74% to 77.6% and that for vaccine b from 71% to 79.6%. abortions occurred 5-10 days post challenge and s. abortusovis was isolated from all aborted fetuses and f ... | 1999 | 10384889 |
| role of the salmonella abortusovis virulence plasmid in the infection of balb/c mice. | following oral inoculation of balb/c mice, salmonella abortusovis strain ss44 was recovered in lower numbers from the peyer's patches and mesenteric lymph nodes compared with s. typhimurium strain sl1344, whereas splenic infections were equivalent between the two serovars. ss44 was cured of its virulence plasmid or subjected to mutagenesis of the spv genes, and the spv(-) derivatives were tested for virulence in mice. plasmid-cured s. abortusovis su40 retained virulence in balb/c mice when inocu ... | 2000 | 10867227 |
| cross-protection of salmonella abortusovis, s. choleraesuis, s. dublin and s. gallinarum in mice induced by s. abortusovis and s. gallinarum: bacteriology and humoral immune response. | cross-protection induced by primary infection with abortusovis and gallinarum was examined against challenge injection with these salmonella serotypes as well as with dublin and choleraesuis, the other virulent serotypes. abortusovis induced efficient protection against the other salmonella. gallinarum was ineffective against choleraesuis. even with low multiplication in mice, the gallinarum j91 strain induced a weak but significant protection against dublin (same o group serotype). the antibodi ... | 2002 | 11873819 |
| genetic parameters for resistance to the salmonella abortusovis vaccinal strain rv6 in sheep. | an experimental population (1216 lambs from 30 sires) of the inra401 sheep was created in an inra flock to allow qtl detection for susceptibility to salmonella infection, wool and carcass traits. the inra401 is a sheep composite line developed from two breeds: berrichon du cher and romanov. at 113 days of age on average, the lambs were inoculated intravenously with 10(8) salmonella abortusovis rv6 (vaccinal strain). they were slaughtered 10 days after the inoculation. several traits were measure ... | 2003 | 12633533 |
| [further study of anti-salmonella abortus ovis specific agglutinins in blood serum of pregnant women]. | | 1955 | 13287999 |
| [abortion in sheep: epidemic salmonella abortusovis outbreak 2005 in switzerland]. | in spring 2005, the outbreak of contagious abortion caused by salmonella abortusovis in 6 sheep flocks in switzerland led to considerable economic losses. the swiss small ruminant health service (bgk) evaluated this case. the aim was to identify the source of the epidemic in order to avoid further spread of infection and to evaluate the possibility of using vaccination. moreover, a strategy for prevention of future outbreaks was developed. this article aims to increase disease awareness of food ... | 2005 | 16259410 |
| migratory monocytes and granulocytes are major lymphatic carriers of salmonella from tissue to draining lymph node. | dendritic cells (dc) are recognized as sentinels, which capture antigens in tissue and migrate to the lymph node, where they initiate immune responses. however, when a vaccine strain of green fluorescent protein-expressing salmonella abortusovis (sao) was inoculated into sheep oral mucosa, it induced accumulation of myeloid non-dc in the subcapsular sinus and paracortex of the draining lymph node, and sao was mainly found associated with these cells (granulocytes and macrophages) but rarely with ... | 2006 | 16330534 |
| molecular genotyping of salmonella enterica abortusovis by pulsed field gel electrophoresis. | genotyping of salmonella strains is an important tool to discriminate among isolates and to improve epidemiological studies when an outbreak occurs. no phagetyping scheme is available for salmonella enterica subsp. enterica serovar abortusovis (sao) and molecular methods previously used were not standardized and were time consuming. among the dna-based methods of genotyping, pulsed field gel electrophoresis (pfge) is currently in use to subtype salmonella isolates. in this study we evaluated the ... | 2006 | 16624505 |
| an outbreak of salmonella abortusovis abortions in sheep in south croatia. | the aim of the study was to give an account of the epidemic of abortions in sheep caused by salmonella enterica ssp. enterica serovar abortusovis, which occurred in dalmatia, south croatia, in winter 2003-2004. five sheep flocks with rate of abortion ranging from 22% to 38% during the last-third of gestation were examined. salmonella abortusovis was isolated from 13 vaginal smears and two fetuses. direct inoculation was found to be superior to pre-enrichment and enrichment in selective broth for ... | 2006 | 16907961 |
| diagnosis by culture and pcr of salmonella abortusovis infection under clinical conditions in aborting sheep in switzerland. | since 2003 eleven swiss sheep flocks were affected by abortion storms due to salmonella abortusovis, an infection which had not been reported in this country for decades although cases of salmonellosis are notifiable in switzerland. this raised doubts about the adequacy of the currently used diagnostic tools and the origin of this infection. therefore, pcr was tested for its potential as a more rapid and more reliable method for diagnosing s. abortusovis infections under field conditions. fecal ... | 2009 | 19403244 |
| diagnosis of placental pathogens in small ruminants by immunohistochemistry and pcr on paraffin-embedded samples. | a histological study was carried out on 58 formalin-fixed and paraffin-embedded samples of placenta from sheep and goats that had aborted, and the placental lesions were graded. sequential histological sections of each cotyledon were then immunostained with specific antibodies and used for pcr detection of chlamydophila abortus, coxiella burnetii, salmonella abortusovis, brucella melitensis, listeria monocytogenes and toxoplasma gondii. most of the cotyledons showed different degrees of placenti ... | 2009 | 19666916 |
| use of an indirect enzyme-linked immunosorbent assay for detection of antibodies in sheep naturally infected with salmonella abortusovis. | an indirect enzyme-linked immunosorbent assay (elisa) was modified and validated to detect antibodies against salmonella abortusovis in naturally infected sheep. the elisa was validated with 44 positive and 45 negative control serum samples. compared with the immunoblot, the sensitivity and specificity of the assay were 98% and 100%, respectively. to follow antibody levels over time, samples from 12 infected ewes were collected at 1, 3, and 10 months after abortion. all animals showed antibody l ... | 2010 | 20622222 |
| seroprevalence survey for salmonella abortusovis infection in swiss sheep flocks. | between 1976 and 2003, no infections with salmonella abortusovis had been officially recorded in switzerland. since then, however, several sheep flocks were infected and suffered massive fetal losses suggesting a re-emergence of the disease. therefore, the aim of this study was to assess the epidemiological situation of s. abortusovis infection in sheep in this country. a representative serum sample collected in 2007 in the context of certifying brucella freedom included sera from 578 flocks wit ... | 2010 | 20870306 |
| functional analysis of bovine nramp1 and production of transgenic cloned embryos in vitro. | natural resistance-associated macrophage protein 1 (nramp1) plays an important role in restraining the growth of intracellular pathogens within macrophages. in this study, nramp1 cdna was cloned from qinchuan cattle and its anti-bacterial activity was demonstrated as being able to significantly inhibit the growth of salmonella abortusovis and brucella abortus in macrophages. calf fibroblasts stably transfected with psp-nramp1-ha vector were used to reconstruct bovine embryos by somatic cell nucl ... | 2015 | 23683995 |
| vaccination schedules in small ruminant farms. | development and implementation of health management plans is the cornerstone of profitable farms; prevention of microbial diseases by means of vaccination is an integral part of such a plan. in every production type and management system in small ruminants, microbial diseases have a major significance, hence their proper control must be based in good health management practices, including use of effective and safe vaccines. development of various types of vaccines is evolving very quickly in rec ... | 2015 | 26220514 |
| fimh adhesin from host unrestricted salmonella enteritidis binds to different glycoprotein ligands expressed by enterocytes from sheep, pig and cattle than fimh adhesins from host restricted salmonella abortus-ovis, salmonella choleraesuis and salmonella dublin. | adhesion to gut tissues and colonization of the alimentary tract, two important stages in the pathogenesis of salmonella, are mediated by fimh adhesin of type 1 fimbriae. it was suggested that minor differences in the structure of fimh are most likely associated with differences in adhesion specificities, and may determine the tropism of various salmonella serovars to different species and tissues. we investigated this hypothesis by comparing the binding properties of fimh proteins from three sa ... | 2013 | 23910950 |
| quantitative trait loci for resistance to infection in sheep using a live salmonella abortusovis vaccine. | quantitative trait loci (qtl) mapping for susceptibility to a salmonella abortusovis vaccinal strain was performed using an experimental design involving 30 romane sheep sire families (1216 progenies). nine qtl corresponding to bacterial load, weight variations and antibody response criteria were mapped on eight chromosomes, including the major histocompatibility complex area on chromosome 20. surprisingly, none was found to be significant in the slc11a1 region (formerly nramp1) that has been sh ... | 2012 | 22497440 |