Publications

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phytochemical and biological activities of bituminaria bituminosa l. (fabaceae).to investigate the phytochemical composition, the antioxidant and antibacterial activities of bituminaria bituminosa l. (fabaceae) (b. bituminosa).201425312171
pterocarpans from bituminaria morisiana and bituminaria bituminosa.the aerial parts of mediterranean papilionaceous plants bituminaria morisiana and b. bituminosa afforded, along with known phenolics, the prenylated pterocarpans bitucarpin a and b, whose structure was elucidated by spectroscopic techniques. a known isoflavonoid (8-prenyldaidzein) was also obtained for the first time as a genuine plant constituent. the accumulation of pterocarpans at the expense of biogenetically more primitive shikimate metabolites like furanocoumarins or isoflavonoids supports ...200312943781
physiological responses of bituminaria bituminosa to heavy metals.two hydroponic experiments were performed to study the physiological responses to heavy metals (hms) of two populations of bituminaria bituminosa (l.) c.h. stirton (fabaceae): one ("c2") from a site contaminated by hms and one from a non-contaminated site ("la"). in the first, we studied the effects of elevated concentrations of zn (12 and 61 μm). population c2 was more tolerant in terms of root and shoot growth at 61 μm zn, relative to control plants (1 μm zn). the similar tissue zn levels of t ...201121937143
accumulation of furanocoumarins by bituminaria bituminosa in relation to plant development and environmental stress.bituminaria bituminosa (l.) c.h. stirton (fabaceae) accumulates high concentrations of the furanocoumarins (fcs) angelicin and psoralen, which protect against infection and herbivory. the effects on fc accumulation of the exposure of two populations of b. bituminosa to abiotic stress (cold, heat and drought) under field conditions were determined, as well as the effect of temperature under controlled conditions, in hydroponic culture. in field conditions, psoralen and angelicin levels in the lea ...201222459509
the use of olive-mill waste compost to promote the plant vegetation cover in a trace-element-contaminated soil.the applicability of a mature compost as a soil amendment to promote the growth of native species for the phytorestoration of a mine-affected soil from a semi-arid area (se spain), contaminated with trace elements (as, cd, cu, mn, pb and zn), was evaluated in a 2-year field experiment. the effects of an inorganic fertiliser were also determined for comparison. bituminaria bituminosa was the selected native plant since it is a leguminous species adapted to the particular local pedoclimatic condit ...201423868726
moderating mycorrhizas: arbuscular mycorrhizas modify rhizosphere chemistry and maintain plant phosphorus status within narrow boundaries.pastures often experience a pulse of phosphorus (p) when fertilized. we examined the role of arbuscular mycorrhizal fungi (amf) in the uptake of p from a pulse. five legumes (kennedia prostrata, cullen australasicum, bituminaria bituminosa, medicago sativa and trifolium subterraneum) were grown in a moderate p, sterilized field soil, either with (+amf) or without (-amf) addition of unsterilized field soil. after 9-10 weeks, half the pots received 15 mg p kg(-1) of soil. one week later, we measur ...201424112081
in vitro cultures of bituminaria bituminosa: pterocarpan, furanocoumarin and isoflavone production and cytotoxic activity evaluation.bituminaria bituminosa l. is known for producing several compounds with considerable pharmaceutical interest, such as phenylpropanoids, furanocoumarins and pterocarpans. in vitro cultures of seedlings, shoots, and callus have been produced to obtain plant materials useful for the production of these metabolites. the secondary metabolite profile was evaluated by hplc-dad. the extracts of all the in vitro material contained the flavonoid daidzein, while plicatin b, erybraedin c and bitucarpin a we ...201424868860
cryopreservation of bituminaria bituminosa varieties and hybrids.bituminaria bituminosa (l.) c.h. stirton is a drought tolerant, perennial legume pasture species and a source of pharmaceutical compounds. bituminaria breeding programs aim to develop and conserve hybrids with desirable traits such as high forage quality, tolerance to biotic or abiotic stresses, and high contents of furanocoumarins. in this work we present a cryopreservation study of different b. bituminosa accessions: two varieties and eight intervarietal hybrids resulting from crosses between ...201526231072
variation in herbage biochemical composition among pitch trefoil (bituminaria bituminosa) populations from elba island, italy.this study assessed the variation in herbage protein and fiber content as well as concentration of furocoumarins, plicatin b, (e)-werneria chromene, and pterocarpans of pitch trefoil (bituminaria bituminosa) germplasm sampled in situ in both summer and autumn in elba island, italy. populations were sampled from a range of climatic and edaphic conditions, on light soils with ph > 7.5. valuable variation occurred for forage quality and chemical composition. the quality features, relevant for a pos ...201626671427
response of spontaneous plants from an ex-mining site of elba island (tuscany, italy) to metal(loid) contamination.the release of large amounts of toxic metals in the neighboring sites of abandoned mine areas represents an important environmental risk for the ecosystem, because it adversely affects soil, water, and plant growth. the aim of the present study was to investigate the metal(loid) (as, cr, cu, ni, pb, and zn) contents of native mediterranean plants grown on the ex-mining area of elba island (italy), with the prospective of its recovery by further phytoremediation technology. soil samples were coll ...201728130721
Next generation DNA sequencing technology delivers valuable genetic markers for the genomic orphan legume species, Bituminaria bituminosa.ABSTRACT: BACKGROUND: Bituminaria bituminosa is a perennial legume species from the Canary Islands and Mediterranean region that has potential as a drought-tolerant pasture species and as a source of pharmaceutical compounds. Three botanical varieties have previously been identified in this species: albomarginata, bituminosa and crassiuscula. B. bituminosa can be considered a genomic 'orphan' species with very few genomic resources available. New DNA sequencing technologies provide an opportuni ...201122171578
erybraedin c, a natural compound from the plant bituminaria bituminosa, inhibits both the cleavage and religation activities of human topoisomerase i.the interaction of human topoisomerase i and erybraedin c, a pterocarpan purified from the plant bituminaria bituminosa, that was shown to have an antitumour activity, was investigated through enzymatic activity assays and molecular docking procedures. erybraedin c is able to inhibit both the cleavage and the religation steps of the enzyme reaction. in both cases, pre-incubation of the drug with the enzyme is required to produce a complete inhibition. molecular docking simulations indicate that, ...201019883377
estradiol-antagonistic activity of phenolic compounds from leguminous plants.natural flavonoids are currently receiving much attention because of their estrogenic and antiestrogenic properties. six isoflavones (isoprunetin, isoprunetin 7-o-beta-d-glucopyranoside, isoprunetin 4',7-di-o-beta-d-glucopyranoside, genistein, genistein 7-o-beta-d-glucopyranoside, daidzein), four flavones (luteolin, luteolin 7-o-beta-d-glucopyranoside, luteolin 4'-o-beta-d-glucopyranoside, licoflavone c), isolated from genista morisii and g. ephedroides (two leguminosae plants of the mediterrane ...200818167044
erybraedin c and bitucarpin a, two structurally related pterocarpans purified from bituminaria bituminosa, induced apoptosis in human colon adenocarcinoma cell lines mmr- and p53-proficient and -deficient in a dose-, time-, and structure-dependent fashion.pterocarpans, the second group of natural isoflavonoids, have received considerable interest on account of their medicinal properties. these drugs are employed as antitoxins, but display antifungal, antiviral and antibacterial properties as well. erybraedin c and bitucarpin a are two new structurally related pterocarpans recently purified and characterized. bitucarpin a differs from erybraedin c for the absence of a prenyl group in 5' position and the presence of a methoxylate hydroxyl group in ...200616271357
anti-clastogenic activity of two structurally related pterocarpans purified from bituminaria bituminosa in cultured human lymphocytes.plant-derived isoflavones are currently receiving much attention because of their phyto-estrogenic, antioxidant, anti-mutagenic, and anti-tumor activities. in this study we have evaluated the clastogenic and anti-clastogenic activities in human lymphocytes of two structurally related pterocarpans, iso-flavonoid derivatives, termed erybraedin c and bitucarpin a, recently purified from bituminaria bituminosa and chemically characterized. mitomycin c (mmc) and the radio-mimetic bleomycin (bl) were ...200415238232
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