Publications

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the orthologue of the fruitfly sex behaviour gene fruitless in the mosquito aedes aegypti: evolution of genomic organisation and alternative splicing.in drosophila melanogaster the doublesex (dsx) and fruitless (fru) regulatory genes act at the bottom of the somatic sex determination pathway. both are regulated via alternative splicing by an upstream female-specific tra/tra-2 complex, recognizing a common cis element. dsx controls somatic sexual differentiation of non-neural as well as of neural tissues. fru, on the other hand, expresses male-specific functions only in neural system where it is required to built the neural circuits underlying ...201323418412
the evolution of female ornaments and weaponry: social selection, sexual selection and ecological competition.ornaments, weapons and aggressive behaviours may evolve in female animals by mate choice and intrasexual competition for mating opportunities-the standard forms of sexual selection in males. however, a growing body of evidence suggests that selection tends to operate in different ways in males and females, with female traits more often mediating competition for ecological resources, rather than mate acquisition. two main solutions have been proposed to accommodate this disparity. one is to expan ...022777016
cost of mating and insemination capacity of a genetically modified mosquito aedes aegypti ox513a compared to its wild type counterpart.the idea of implementing genetics-based insect control strategies modelled on the traditional sit is becoming increasingly popular. in this paper we compare a genetically modified line of aedes aegypti carrying a tetracycline repressible, lethal positive feedback system (ox513a) with its wild type counterpart with respect to their insemination capacities and the cost of courtship and mating. genetically modified males inseminated just over half as many females as the wild type males during their ...201122022518
male mating costs in a polygynous mosquito with ornaments expressed in both sexes.male mate choice in species with conventional sex roles is difficult to explain and has, therefore, been the focus of many recent theoretical models. these models have focused on variance in female quality and, to a lesser extent, male investments/costs associated with mating. in this study, we investigate the costs of courtship and copulation in the polygynous mosquito sabethes cyaneus. in this species, both males and females possess elaborate ornaments. previous studies suggest that the most l ...200919640881
the effects of interspecific courtship on the mating success of aedes aegypti and aedes albopictus (diptera: culicidae) males.satyrization, a form of asymmetric reproductive interference, has recently been shown to play a role in competitive displacements of aedes aegypti (l.) by aedes albopictus (skuse). furthermore, female ae. aegypti from populations in sympatry with ae. albopictus have evolved reproductive character displacement and changes in mating behavior to reduce interspecific mating. in this article, we examine evolutionary responses of males to interspecific mating and show that satyrization has also evoked ...201527418696
phylogenetic analysis and temporal diversification of mosquitoes (diptera: culicidae) based on nuclear genes and morphology.phylogenetic analyses provide a framework for examining the evolution of morphological and molecular diversity, interpreting patterns in biogeography, and achieving a stable classification. the generic and suprageneric relationships within mosquitoes (diptera: culicidae) are poorly resolved, making these subjects difficult to address.200920028549
molecular identification of saint louis encephalitis virus genotype iv in colombia.saint louis encephalitis virus (slev) is a member of the japanese-encephalitis virus serocomplex of the genus flavivirus. slev is broadly distributed in the americas and the caribbean islands, where it is usually transmitted by mosquitoes of the genus culex and primarily to birds and mammalian-hosts. humans are occasionally infected by the virus and are dead-end hosts. slev causes encephalitis in temperate regions, while in tropical regions of the americas, several human cases and a wide biologi ...201526313538
colonized sabethes cyaneus, a sylvatic new world mosquito species, shows a low vector competence for zika virus relative to aedes aegypti.the introduction of zika virus (zikv) to the americas raised concern that the virus would spill back from human transmission, perpetuated by aedes aegypti, into a sylvatic cycle maintained in wildlife and forest-living mosquitoes. in the americas, sabethes species are vectors of sylvatic yellow fever virus (yfv) and are therefore candidate vectors of a sylvatic zikv cycle. to test the potential of sabethes cyaneus to transmit zikv, sa. cyaneus and ae. aegypti were fed on a129 mice one or two day ...201830115888
diversity of mosquito vectors (diptera: culicidae) in caxiuanã, pará, brazil.this paper presents a study based on ecological parameters represented by diversity and richness indices applied in a community of mosquitoes (diptera: culicidae), at the national forest of caxiuanã, melgaço municipality, state of pará, in the brazilian amazon. a total of 25,433 specimens of culicids were collected in the study, from five field collection periods, over 10 months, between 2005 and 2006. specimens were collected in four heights of the forest (ground level, 8 m, 16 m, and 30 m-cano ...201222997514
interactions between coexisting intracellular genomes: mitochondrial density and wolbachia infection.many arthropods are infected with maternally transmitted microorganisms, leading to the coexistence of several intracellular genomes within the host cells, including their own mitochondria. as these genomes are cotransmitted, their patterns of evolution have been intimately linked, with possible consequences for the diversity and evolution of the host mitochondrial dna. the evolutionary aspects of the situation have been thoroughly investigated, especially the selective sweep on the mitochondria ...200919181828
cytogenetic and isozyme profile of sabethes cyaneus: a mosquito of the neotropical canopy.sabethes cyaneus, a newly colonized culicine mosquito from the panamanian forest canopy, has a distribution range from honduras to argentina. cytogenetic studies, the first on any sabethes species, revealed a karyotype of three pairs of homomorphic chromosomes (2n = 6). well-developed polytene chromosomes were discovered in the larval salivary glands and a photomap standard was constructed. clear banding patterns and consistent landmarks distinguished each of the six arms. substantial asynapsis ...20072876025
scanning electron microscopy of eggs of sabethes cyaneus.mosquitoes of the neotropical genus sabethes, some species of which are yellow fever vectors, most often develop through the immature stages in tree holes. sabethes eggs have not been previously characterized using scanning electron microscopy. eggs of sabethes cyaneus (length: 349.6 +/- 2.7 microm; width: 172.6 +/- 1.14 microm; n = 10) are almost biconical when examined from the top. from a lateral perspective 2 surfaces can be seen. one surface is smooth and more convex, whereas the other is l ...201323687859
tests of sabethes cyaneus leg paddle function in mating and flight.both sexes of the mosquito sabethes cyaneus have conspicuous midleg paddles of unknown function. these paddles are displayed prominently by males during courtship. however, we found that paddle removal reduced successful mating of females, not males. males without paddles approached and courted females normally, but females without paddles were rarely approached by males. paddle removal appeared not to alter flight, and paddleless females oviposited in the manner characteristic of sabethes, sugg ...19901983023
chromosomal localization and copy number of 18s + 28s ribosomal rna genes in evolutionarily diverse mosquitoes (diptera, culicidae).in situ hybridization using 3h-labeled 18s and 28s ribosomal dna (rdna) probes from aedes albopictus was performed on the mitotic chromosomes of 20 species of mosquitoes belonging to 8 genera of subfamilies culicinae and anophelinae. in all but one species examined, the rdna family was localized to a single chromosome per haploid genome. aedes triseriatus was the only exception, with the rdna cistrons present on chromosome 1 and on chromosome 3. the ribosomal rna genes were located on chromosome ...19902093704
experimental studies to determine the susceptibility to infection with st. louis encephalitis virus of five species of panamanian mosquitoes.the susceptibility to infection with a panamanian isolate of st. louis encephalitis (sle) virus was evaluated in culex quinquefasciatus, haemagogus equinus, mansonia dyari, sabethes cyaneus and deinocerites pseudes. when fed on blood-virus suspensions using the hanging drop or pledget technique, the median infective dose (id50) of sle virus for colonized strains of cx. quinquefasciatus and hg. equinus was 10(2.8) plaque forming units (pfu)/2 microliters and 10(3.5) pfu/2 microliters, respectivel ...19911686273
release-recapture experiments with canopy mosquitoes in the genera haemagogus and sabethes (diptera: culicidae) in brazilian amazonia.in 2 forested areas near belém (para state, brazil), 2 haemagogus and 6 sabethes species were marked released and recaptured in may 1989 and in april 1993. the recapture rates were high, 4.9 and 13.1% for haemagogus and sabethes spp., respectively. for haemagogus janthinomys dyar, females were recaptured until 27 d after release. the duration of the gonotrophic cycle was between 5.0 and 9.5 d and the survival rate was 0.90-0.92. haemagogus leucocelaenus (dyar & shannon) was recaptured once, 21 d ...19989835682
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