| quantitative analysis of phytopathogenic ascomycota on leaves of pedunculate oaks (quercus robur l.) by real-time pcr. | leaves of oak trees are often infected by various pathogenic fungi. as it is difficult to remove such organisms quantitatively from the leaf surface and as it is often impossible to grow these organisms independently from their host, there are almost no molecular data available from these oak leaf specific pathogens. for the quantitative removal of the microorganisms a procedure was developed combining a wax and microorganism freezing method with a dna extraction technique. for the development o ... | 2002 | 12007821 |
| proposal of nakamurella gen. nov. as a substitute for the bacterial genus microsphaera yoshimi et al. 1996 and nakamurellaceae fam. nov. as a substitute for the illegitimate bacterial family microsphaeraceae rainey et al. 1997. | the bacterial genus microsphaera yoshimi et al. 1996 is illegitimate because of priority of the fungal genus microsphaera (wallr.) lév. [principle 2, rule 51b(4) of the bacteriological code]. therefore, a new genus name, nakamurella, is proposed for the bacterial genus. the type species microsphaera multipartita yoshimi et al. 1996 becomes nakamurella multipartita gen. nov., comb. nov. due to the illegitimacy of the only genus in the family microsphaeraceae rainey et al. 1997, this family name i ... | 2004 | 15143055 |
| new and remarkable microfungi in north rhine westphalia, germany. | during our investigation on microfungi in north rhine westphalia in the years 2002 and 2003 we were able to collect and identify some new and rare species of microfungi as parasites and saprophytes on wild and ornamental plants. some of these like erysiphe elevata (burill.) u. braun & s. takamatsu comb. nov. [=microsphaera elevata burill.] on catalpa bignonioides walt., erysiphe syringae-japonicae (u. braun) u. braun & s. takamatsu [= microsphaera syringae-japonicae u. braun, m. aceris bunkina. ... | 2004 | 15756829 |
| quadrisphaera granulorum gen. nov., sp. nov., a gram-positive polyphosphate-accumulating coccus in tetrads or aggregates isolated from aerobic granules. | a gram-positive bacterium, designated strain ag019(t), was isolated by micromanipulation from aerobic granules obtained from a laboratory-scale sequencing batch reactor. this isolate grew axenically as cocci clustered predominantly in tetrads, and was morphologically similar to the dominant organisms observed in the biomass. the morphology also resembled that of the tetrad-forming organisms commonly seen in activated sludge samples. strain ag019(t) was found to be an oxidase-negative, catalase-p ... | 2005 | 16166665 |
| caespitotheca gen. nov., an ancestral genus in the erysiphales. | the phylogenetic position of uncinula forestalis within the erysiphales has been inferred from 5.8s, 18s, and 28s rdna sequences. although the appendages of the ascomata are uncinula-like, i.e. unbranched with curved-coiled apices, u. forestalis is situated at the very base of the large erysiphales cluster, far from the 'pseudoidium' clade (erysiphe, including microsphaera and uncinula) and separate from the recently introduced basal genus parauncinula. u. forestalis differs morphologically from ... | 2005 | 16175792 |
| nod factor induces soybean resistance to powdery mildew. | plants possess highly sensitive perception systems by which microbial signal molecules are recognized. in the bradyrhizobium-soybean (glycine max (l.) merr.) symbiosis, recognition is initiated through exchange of signal molecules, generally flavonoids from soybean and lipo-chitooligosaccharides (nod factors) from the microsymbiont. application of the nod factor nod bj-v (c18:1, mefuc) induced soybean resistance to powdery mildew caused by microsphaera diffusa. addition of nod factor (concentrat ... | 2005 | 16310369 |
| humicoccus flavidus gen. nov., sp. nov., isolated from soil. | a gram-positive, non-motile, spherical, non-spore-forming bacterial strain, ds-52(t), was isolated from soil from dokdo, korea, and its taxonomic position was investigated by using a polyphasic approach. it grew optimally at 25 degrees c and ph 6.0-7.0. strain ds-52(t) had meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan, and galactose, mannose, xylose and rhamnose as whole-cell sugars. it contained mk-8(h(4)) and mk-9(h(4)) as the predominant menaquinones a ... | 2007 | 17220441 |
| comparison of its sequences from uk and north american sugar-beet powdery mildews and the designation of erysiphe betae. | powdery mildew of sugar beet, a disease of major economic significance, was first described at the beginning of the 20th century, and since then there has been some confusion over the correct taxonomic identity of the causal agent. in europe, the fungus was initially classified as the novel species microsphaera betae, later re-named erysiphe betae, whilst in america it was identified as e. polygoni, despite sugar-beet isolates from both regions having a host range restricted to beta species. it ... | 2007 | 17324758 |
| erysiphe abbreviata on cherry bark oak--morphology, phylogeny and taxonomy. | powdery mildew on cherry bark oak (quercus falcate var. pagodifolia) collected in tennessee, usa, was determined to be erysiphe abbreviata, a species confined to north america. the diagnostically important anamorph of this species is described for the first time. sequence analyses of the rdna its region and d1/d2 domains of the 28s rdna were used to obtain phylogenetic data for and taxonomic conclusions about this species. the structure of the anamorph (oidium subgen. pseudoidium) and the molecu ... | 2007 | 18268900 |
| saxeibacter lacteus gen. nov., sp. nov., an actinobacterium isolated from rock. | a gram-positive, cream-coloured, non-motile, rod-shaped actinomycete, designated dls-10t, was isolated from a rock collected on the peak of darangshi oreum (a small mountain 300 m above sea level) in jeju, republic of korea. the temperature and ph ranges for growth were 4-37 degrees c and ph 5.1-9.1. the organism grew at nacl concentrations up to 3 % and had the following chemotaxonomic characteristics: meso-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan, the ... | 2008 | 18398192 |
| identification of individual powdery mildew fungi infecting leaves and direct detection of gene expression by single conidium polymerase chain reaction. | abstract greenhouse-grown tomato seedlings were inoculated naturally with two genera of powdery mildew conidia forming appressorial germ tubes that could not be differentiated by length alone. for direct identification, single germinated conidia were removed from leaves by means of a glass pipette linked to the manipulator of a high-fidelity digital microscope. this microscope enabled in vivo observation of the fungi without leaf decoloration or fungal staining. the isolated conidia were subject ... | 2005 | 18943465 |
| genetic map of the powdery mildew resistance gene in soybean pi 243540. | powdery mildew (caused by microsphaera diffusa cooke & peck) is a common disease of soybean in many soybean-growing regions of the world and under greenhouse conditions. the previously reported rmd locus of soybean for resistance to powdery mildew was mapped on soybean molecular linkage group j (chromosome 16). we have discovered a single dominant gene in pi 243540 that provides season-long resistance to powdery mildew. the objective of this study was to map the powdery mildew resistance gene in ... | 2010 | 20616870 |
| genetic variability and mycohost association of ampelomyces quisqualis isolates inferred from phylogenetic analyses of its rdna and actin gene sequences. | ampelomyces quisqualis complex is well known as the most common and widespread hyperparasite of the family erysiphaceae, the cause of powdery mildew diseases. as commercial biopesticide products it is widely used to control the disease in field and plastic houses. although genetic diversity within ampelomyces isolates has been previously recognized, a single name a. quisqualis is still applied to all pycnidial intracellular hyperparasites of powdery mildew fungi. in this study, the phylogenetic ... | 2010 | 20943134 |
| complete genome sequence of nakamurella multipartita type strain (y-104). | nakamurella multipartita (yoshimi et al. 1996) tao et al. 2004 is the type species of the monospecific genus nakamurella in the actinobacterial suborder frankineae. the nonmotile, coccus-shaped strain was isolated from activated sludge acclimated with sugar-containing synthetic wastewater, and is capable of accumulating large amounts of polysaccharides in its cells. here we describe the features of the organism, together with the complete genome sequence and annotation. this is the first complet ... | 2010 | 21304699 |
| identification and structure of the mating-type locus and development of pcr-based markers for mating type in powdery mildew fungi. | in ascomycetes, mating compatibility is regulated by the mating-type locus, mat1. the objectives of this study were to identify and sequence genes at the mat1 locus in the grape powdery mildew fungus, erysiphe necator, to develop a pcr-based marker for determining mating type in e. necator, and to develop degenerate primers for amplification by pcr of conserved regions of mating-type idiomorphs in other powdery mildew fungi. we identified mat1-2-1 of the mat1-2 idiomorph in e. necator based on t ... | 2011 | 21515399 |