| the genus nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of nocardiopsaceae fam. nov. | the genus nocardiopsis was shown to be phylogenetically coherent and to represent a distinct lineage within the radiation of the order actinomycetales. the closest relatives of the genus nocardiopsis are members of the genera actinomadura, thermomonospora, streptosporangium, and microtetraspora. the intrageneric structure of the genus nocardiopsis is shown to consist of a highly related species group containing nocardiopsis dassonvillei, nocardiopsis alborubida, and nocardiopsis antarctica and a ... | 1996 | 8863440 |
| description of nocardiopsis synnemataformans sp. nov., elevation of nocardiopsis alba subsp. prasina to nocardiopsis prasina comb. nov., and designation of nocardiopsis antarctica and nocardiopsis alborubida as later subjective synonyms of nocardiopsis dassonvillei. | data from chemotaxonomic and 16s ribosomal dna sequence analyses of an isolate obtained from the sputum of a kidney transplant patient identified the isolate as a member of the genus nocardiopsis. dna-dna hybridization data, as well as physiological characteristics, indicated that the isolate represents a new species of the genus nocardiopsis, designated nocardiopsis synnemataformans; the type strain is strain immib d-1215 (= dsm 44143). in addition, dna-dna hybridization data, as well as the re ... | 1997 | 9336896 |
| nocardiopsis compostus sp. nov., from the atmosphere of a composting facility. | three strains (ks8, ks9t and ks21), isolated from air samples near a composting facility, were subjected to taxonomic analyses (characterized using a polyphasic approach). morphological and chemotaxonomic characteristics of the isolates were in agreement with those described for members of the genus nocardiopsis. on the basis of 16s rrna sequence comparison and phenotypic tests, ks21 clearly belonged to nocardiopsis alba. ks8 and ks9t showed less than 98% 16s rrna gene sequence similarity to any ... | 2002 | 11931176 |
| nocardiopsis aegyptia sp. nov., isolated from marine sediment. | an actinomycete, strain sng49(t), was isolated from marine sediment of abu qir bay, on the western seashore of alexandria, egypt. the bacterium was aerobic and gram-positive. it produced beige to light-yellow aerial mycelium, brown substrate mycelium and straight to flexuous hyphae, but no specific spore chains. 16s rdna sequence analysis and chemotaxonomic markers were consistent with classification of strain sng49(t) in the genus nocardiopsis, i.e. meso-diaminopimelic acid; no diagnostic sugar ... | 2004 | 15023959 |
| [farmer's lung cases of a farmer and his son with high bal fluid beta-d glucan levels]. | a farmer and his son, who treated straw in a cowshed, were admitted to our hospital because of severe dyspnea during summer time. their chest x-ray films revealed bilateral reticulonodular shadows in the middle to lower lung fields. bronchoalveolar lavage (bal) fluid analyses showed a high proportion of lymphocytes and an increased cd4/8 ratio. they were diagnosed with farmer's lung and treated with pulse therapy with methylprednisolone and tapering of steroid. hypoxemia and interstitial shadow ... | 2004 | 15678910 |
| [cell wall teichoic acids from streptomyces daghestanicus vkm ac-1722t and streptomyces murinus ina-00524t]. | the structure of cell wall teichoic acids was studied by chemical methods and nmr spectroscopy in the type strains of two actinomycete species of the "streptomyces griseoviridis" phenetic cluster: streptomyces daghestanicus and streptomyces murinus. s. daghestanicus vkm ac-1722t contained two polymers having a 1,5-poly(ribitol phosphate) structure. in one of them, the ribitol units had alpha-rhamnopyranose and 3-o-methyl-alpha-rhamnopyranose substituents; in the other, each ribitol unit was carr ... | 2005 | 15835778 |
| structural and mechanistic exploration of acid resistance: kinetic stability facilitates evolution of extremophilic behavior. | kinetically stable proteins are unique in that their stability is determined solely by kinetic barriers rather than by thermodynamic equilibria. to better understand how kinetic stability promotes protein survival under extreme environmental conditions, we analyzed the unfolding behavior and determined the structure of nocardiopsis alba protease a (napase), an acid-resistant, kinetically stable protease, and compared these results with a neutrophilic homolog, alpha-lytic protease (alphalp). alth ... | 2007 | 17382344 |
| production and characterization of lipopeptide biosurfactant by a sponge-associated marine actinomycetes nocardiopsis alba msa10. | a sponge-associated marine actinomycetes nocardiopsis alba msa10 was screened and evaluated for the production of biosurfactant. biosurfactant production was confirmed by conventional screening methods including hemolytic activity, drop collapsing test, oil displacement method, lipase production and emulsification index. the active compound was extracted with three solvents including ethyl acetate, diethyl ether and dichloromethane. the diethyl ether extract was fractionated by tlc and semi-prep ... | 2009 | 19288138 |
| isolation and characterization of a nocardiopsis sp. from honeybee guts. | although actinomycetes are the plant-associated environmental bacteria best known for producing thousands of antibiotics, their presence in the guts of flower-feeding honeybees has rarely been reported. here, we report on the selective isolation of actinomycetes from the gut microbiota of healthy honeybees, and their inhibitory activity against honeybee indigenous bacteria. more than 70% of the sampled honeybees (n>40) in a season carried at least one cfu of actinomycete. the isolates from bees ... | 2010 | 20846361 |
| nocardiopsis yanglingensis sp. nov., a thermophilic strain isolated from a compost of button mushrooms. | a strain named a18 was recovered from a compost of button mushrooms. it was characterized using a polyphasic approach. on the basis of 16s rrna gene sequence comparison, it belonged to the genus nocardiopsis and was most closely related to the type strains of nocardiopsis flavescens (sequence similarity 98.0%), nocardiopsis prasina (97.5%), nocardiopsis metallicus (97.4%), nocardiopsis alba (97.3%). the combination of phylogenetic analysis, dna-dna hybridization, phenotypic characteristics and c ... | 2011 | 21671196 |
| melanin-templated rapid synthesis of silver nanostructures. | as a potent antimicrobial agent, silver nanostructures have been used in nanosensors and nanomaterial-based assays for the detection of food relevant analytes such as organic molecules, aroma, chemical contaminants, gases and food borne pathogens. in addition silver based nanocomposites act as an antimicrobial for food packaging materials. in this prospective, the food grade melanin pigment extracted from sponge associated actinobacterium nocardiopsis alba msa10 and melanin mediated synthesis of ... | 2014 | 24885756 |
| an airborne actinobacteria nocardiopsis alba isolated from bioaerosol of a mushroom compost facility. | actinobacteria are widely distributed in many environments and represent the most important trigger to the occupant respiratory health. health complaints, including hypersensitivity pneumonitis of the workers, were recorded in a mushroom compost facility (mcf). the studies on the airborne bacteria were carried out to find a possible microbiological source of these symptoms. culture analysis of compost bioaerosols collected in different location of the mcf was performed. an assessment of the indo ... | 2014 | 25382928 |
| molecular expression of l-asparaginase gene from nocardiopsis alba niot-vkma08 in escherichia coli: a prospective recombinant enzyme for leukaemia chemotherapy. | l-asparaginase is an antineoplastic agent that selectively reduces the level of l-asparagine in blood and diminishes the proliferation of cancerous cells. studies were carried out on the cloning and heterologous expression of l-asparaginase biosynthesis gene (ansa) from nocardiopsis alba niot-vkma08 to achieve the stable inducible system that overproduces the glutaminase-free recombinant l-asparaginase. overexpression of recombinant l-asparaginase was achieved with an optimized final concentrati ... | 2016 | 27155523 |
| novel glutaminase free l-asparaginase from nocardiopsis alba niot-vkma08: production, optimization, functional and molecular characterization. | studies were carried out for the optimization and production of novel extracellular glutaminase-free l-asparaginase from nocardiopsis alba niot-vkma08. among the tested carbon and nitrogen sources, maximum l-asparaginase production was observed with a combination of l-asparagine and maltose (1.5%) and twofold increase in yield (18.47 iu ml(-1)) was observed with newly optimized niot-asparaginase medium. activity of the purified enzyme was moderately inhibited by various divalent cations and thio ... | 2015 | 25274019 |
| isomethoxyneihumicin, a new cytotoxic agent produced by marine nocardiopsis alba km6-1. | a new cytotoxic agent designated isomethoxyneihumicin (1 and 2), a mixture of lactam-lactim tautomers, was isolated along with methoxyneihumicin (3) from the culture broth of the marine nocardiopsis alba km6-1. the structures of 1 and 2 were elucidated in spectroscopic analyses (1d and 2d nmr data, and roesy correlations). isomethoxyneihumicin (15.0 μm) and 3 (15.0 μm) arrested the cell cycle of jurkat cells at the g2/m phase (66 and 67%) in 12 h. isomethoxyneihumicin and 3 exhibited cytotoxicit ... | 2016 | 27999443 |
| quinazoline derivative from indigenous isolate, nocardiopsis alba inhibits human telomerase enzyme. | aim of this study was isolation and screening of various secondary metabolites produced by indigenous isolates of soil actinomycetes for human telomerase inhibitory activity. | 2016 | 27567126 |
| cloning, expression and characterization of a novel gh5 exo/endoglucanase of thermobifida halotolerans yim 90462(t) by genome mining. | the 1389-bp thcel5a gene, which encodes a family 5 of glycoside hydrolases (gh5), was screened from the draft genome of thermobifida halotolerans yim 90462(t). thcel5a was most similar (77% identity) to a gh5 endoglucanase from thermobifida fusca yx, followed by cellulases from nocardiopsis dassonvillei subsp. dassonvillei dsm 43111, nocardiopsis alba atcc baa-2165, and kribbella flavida dsm 17836. the deduced amino acid sequence of thcel5a, which consisted of 462 amino acid residues, encompassed ... | 2015 | 26026381 |
| nocardiopsis algeriensis sp. nov., an alkalitolerant actinomycete isolated from saharan soil. | an alkalitolerant actinomycete strain, designated b32(t), was isolated from a saharan soil sample collected from adrar province (south of algeria), and then investigated using a polyphasic taxonomic approach. the strain was observed to produce short chains of spores on the dichotomous branched aerial mycelium and formed a fragmented substrate mycelium. the optimum nacl concentration for growth was found to be 0-5 % (w/v) and the optimum growth temperature and ph were found to be 25-35 °c and 7.0 ... | 2015 | 25380720 |
| thermodynamics of a ca(2+)-dependent highly thermostable alkaline protease from a haloalkliphilic actinomycete. | an alkaline protease from salt-tolerant alkaliphilic actinomycetes, nocardiopsis alba ok-5 was purified by a single-step hydrophobic interaction chromatography and characterized. the purified protease with an estimated molecular mass of 20 kda was optimally active at 70 °c in 0-3 m nacl and 0-100 mm ca(2+) displaying significant stability at 50-80 °c. the enzyme was stable at 80 °c in 100 mm ca(2+) with kd of 17 × 10(-3) and t1/2 of 32 min. the activation energy (ea), enthalpy (δh*), and entropy ... | 2015 | 25150113 |
| analysis of the biosynthesis of antibacterial cyclic dipeptides in nocardiopsis alba. | nocardiopsis alba is frequently isolated from environment and has recently been suggested as a casual symbiotic actinobacterium of diverse invertebrates. using activity-guided fractionation, we purified two antibacterial cyclic dipeptides, cyclo(δphe-δleu) (albonoursin) and cyclo(δmtyr-δleu), from a culture of nocardiopsis alba atcc baa-2165. analysis of n. alba genome revealed genetic information similar to albonoursin biosynthetic gene cluster, albabc. an albabc gene deletion mutant of n. alba ... | 2014 | 25048158 |
| production of bioactive compounds by actinomycetes and their antioxidant properties. | an actinomycete was isolated from mangrove soil collected from nellore region of andhra pradesh, india, and screened for its ability to produce bioactive compounds. the cultural, morphological, and biochemical characters and 16s rrna sequencing suggest that the isolated strain is nocardiopsis alba. the bioactive compounds produced by this strain were purified by column chromatography. the in vitro antioxidant capacity of the isolated compounds (fractions) was estimated and fraction f2 showed ver ... | 2014 | 24790761 |
| a bacterial source for mollusk pyrone polyketides. | in the oceans, secondary metabolites often protect otherwise poorly defended invertebrates, such as shell-less mollusks, from predation. the origins of these metabolites are largely unknown, but many of them are thought to be made by symbiotic bacteria. in contrast, mollusks with thick shells and toxic venoms are thought to lack these secondary metabolites because of reduced defensive needs. here, we show that heavily defended cone snails also occasionally contain abundant secondary metabolites, ... | 2013 | 23352141 |
| reliable and reproducible method for rapid identification of nocardia species by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. | recently, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms) has been challenged for the identification of nocardia species. however, the standard ethanol-formic acid extraction alone is insufficient in allowing the membrane proteins of nocardia species to be ionized by the matrix. we therefore aimed to establish our new extraction method for the maldi-tof ms-based identification of nocardia species isolates. our modified extraction procedure is through d ... | 2013 | 24800394 |
| whole-genome sequence of nocardiopsis alba strain atcc baa-2165, associated with honeybees. | the actinomycete nocardiopsis alba was reportedly associated with honeybees in separate occurrences. we report the complete genome of nocardiopsis alba atcc baa-2165 isolated from honeybee guts. it will provide insights into the metabolism and genetic regulatory networks of this genus of bacteria that enable them to live in a range of environments. | 2012 | 23105086 |
| new diketopiperazine derivatives from a deep-sea-derived nocardiopsis alba scsio 03039. | the strain scsio 03039 was isolated from a sediment sample in the indian ocean and was characterized as a nocardiopsis alba species on the basis of its 16s rrna gene sequence. seven diketopiperazines (dkps), including two new dkps nocazines d (2a) and e (2b), and five known dkps (1, 3-6), were isolated from n. alba scsio 03039, along with two known compounds 2-methoxy-1,4-naphthoquinone (7) and 1-hydroxy-4-methoxy-2-naphthoic acid (8). their structures were elucidated by mass and nmr spectroscop ... | 2013 | 23093033 |
| purification strategies, characteristics and thermodynamic analysis of a highly thermostable alkaline protease from a salt-tolerant alkaliphilic actinomycete, nocardiopsis alba ok-5. | an alkaline protease from salt tolerant alkaliphilic actinomycetes, nocardiopsis alba strain ok-5 was purified to homogeneity by 27 and 13 fold with a yield of 35 and 13% using two-steps and one-step method, respectively. the purification methods involved hydrophobic interaction on phenyl sapharose matrix. the apparent molecular mass was 20 kda. the temperature optimum shifted from 70 to 80°c in 4m nacl and 30% na-glutamate, with significant stability at 60-80°c in na-glutamate. deactivation rat ... | 2012 | 22377407 |