| nocardia concava sp. nov., isolated from japanese patients. | two actinomycete strains, ifm 0354(t) and ifm 0576, isolated from japanese patients, were found to have morphological, biochemical and chemotaxonomic properties consistent with their classification in the genus nocardia. the strains resembled nocardia otitidiscaviarum and nocardia uniformis in their phenotypic properties. comparative 16s rrna gene sequence analysis showed that the strains are closely related to nocardia seriolae. dna-dna relatedness values and phenotypic differences from n. seri ... | 2005 | 16166713 |
| non-ribosomal propeptide precursor in nocardicin a biosynthesis predicted from adenylation domain specificity dependent on the mbth family protein noci. | nocardicin a is a monocyclic β-lactam isolated from the actinomycete nocardia uniformis that shows moderate antibiotic activity against a broad spectrum of gram-negative bacteria. the monobactams are of renewed interest due to emerging gram-negative strains resistant to clinically available penicillins and cephalosporins. like isopenicillin n, nocardicin a has a tripeptide core of non-ribosomal origin. paradoxically, the nocardicin a gene cluster encodes two non-ribosomal peptide synthetases (nr ... | 2013 | 23330869 |
| mutational analysis of nock and nocl in the nocardicin a producer nocardia uniformis. | the nocardicins are a family of monocyclic beta-lactam antibiotics produced by the actinomycete nocardia uniformis subsp. tsuyamanensis atcc 21806. the most potent of this series is nocardicin a, containing a syn-configured oxime moiety, an uncommon feature in natural products. the nocardicin a biosynthetic gene cluster was recently identified and found to encode proteins in keeping with nocardicin a production, including the nocardicin n-oxygenase, nocl, in addition to genes of undetermined fun ... | 2005 | 15629944 |
| nocardia acidivorans sp. nov., isolated from soil of the island of stromboli. | a gram-positive, non-spore-forming bacterium (strain gw4-1778(t)) was isolated from soil of the italian island of stromboli. 16s rrna gene sequence similarity studies showed that strain gw4-1778(t) is a member of the genus nocardia, most closely related to nocardia pseudobrasiliensis (genbank accession no. dq659914; 98.6 %), nocardia nova (z36930; 98.6 %), nocardia niigatensis (ab092563; 98.4 %), nocardia jiangxiensis (ay639902; 98.0 %), nocardia uniformis (z46752; 98.0 %) and nocardia miyunensi ... | 2007 | 17551026 |
| identification and characterization of nocr as a positive transcriptional regulator of the beta-lactam nocardicin a in nocardia uniformis. | nocardicin a is a monocyclic beta-lactam isolated from the actinomycete nocardia uniformis, which shows moderate activity against a broad spectrum of gram-negative bacteria. within the biosynthetic gene cluster of nocardicin a, nocr encodes a 583-amino-acid protein with high similarity to a class of transcriptional regulators known as streptomyces antibiotic regulatory proteins. insertional inactivation of this gene resulted in a mutant showing morphology and growth characteristics similar to th ... | 2009 | 19028891 |
| mutational analysis and characterization of nocardicin c-9' epimerase. | the biosynthetic gene cluster for the nocardicin a producer nocardia uniformis subsp. tsuyamanensis atcc 21806 was recently identified. nocardicin a is the most potent of a series of monocyclic beta-lactam antibiotics produced by this organism. its activity has been attributed to a syn-configured oxime moiety and a d-homoseryl side chain attached through an unusual ether linkage to the core nocardicin framework. notably present in the nocardicin biosynthetic gene cluster is nocj, encoding a prot ... | 2004 | 15252031 |
| the biosynthetic gene cluster for a monocyclic beta-lactam antibiotic, nocardicin a. | the monocyclic beta-lactam antibiotic nocardicin a is related structurally and biologically to the bicyclic beta-lactams comprised of penicillins/cephalosporins, clavams, and carbapenems. biosynthetic gene clusters are known for each of the latter, but not for monocyclic beta-lactams. a previously cloned gene encoding an enzyme specific to the biosynthetic pathway was used to isolate the nocardicin a cluster from nocardia uniformis. sequence analysis revealed the presence of 14 open reading fram ... | 2004 | 15271351 |
| role of the cytochrome p450 nocl in nocardicin a biosynthesis. | the nocardicins are members of a class of monocyclic beta-lactam antibiotics produced by the actinomycete nocardia uniformis subsp. tsuyamanesis (atcc 21806). the oxime moiety in nocardicin a is required for full biological activity and is rare among natural products. a putative gene cluster encoding the enzymes of the nocardicin biosynthetic pathway has been identified. among these, nocl shows similarity to cytochromes p450. we describe its heterologous expression and demonstrate its role in th ... | 2002 | 12105888 |
| nocardia uniformis nom. rev. | a soil isolate representing the putatively novel species 'nocardia uniformis' was found to have morphological, staining and chemotaxonomic properties consistent with its classification in the genus nocardia. an almost complete sequence of the 16s rdna of the strain was determined following cloning and sequencing of the amplified gene. the sequence was aligned with those available for nocardiae and phylogenetic trees were inferred using four tree-making algorithms. the organism was consistently a ... | 1999 | 10425784 |
| nocardia uniformis, a new species from solonetz soil. | | 1959 | 14421871 |
| nocardia inohanensis sp. nov., nocardia yamanashiensis sp. nov. and nocardia niigatensis sp. nov., isolated from clinical specimens. | comparative 16s rdna studies on six strains of actinomycete isolated from clinical specimens revealed that they belong to the genus nocardia and are closely related to nocardia seriolae, nocardia otitidiscaviarum, nocardia uniformis, nocardia pseudobrasiliensis and nocardia crassostreae. however, the novel organisms consistently formed a clade distinct from that of the five latter species. determination of dna-dna relatedness indicated that these strains could be classified under three novel spe ... | 2004 | 15023976 |
| purification, characterization, and cloning of an s-adenosylmethionine-dependent 3-amino-3-carboxypropyltransferase in nocardicin biosynthesis. | s-adenosylmethionine:nocardicin 3-amino-3-carboxypropyltransferase catalyzes the biosynthetically rare transfer of the 3-amino-3-carboxypropyl moiety from s-adenosylmethionine to a phenolic site in the beta-lactam substrates nocardicin e, f, and g, a late step of the biosynthesis of the monocyclic beta-lactam antibiotic nocardicin a. whereas a number of conventional methods were ineffective in purifying the transferase, it was successfully obtained by two complementary affinity chromatography st ... | 1998 | 9804844 |
| nocardicin a, a new monocyclic beta-lactam antibiotic. i. discovery, isolation and characterization. | nocardicin a is a new monocyclic beta-lactam antibiotic obtained from the fermentation broth of a strain of actinomycetes. the producing organism, strain ws 1571, was identified as nocardia uniformis subsp. tsuyamanensis atcc 21806. the antibiotic, obtained as colorless crystals, exhibits moderate in vitro antibacterial activity against a broad-spectrum of gram-negative bacteria including proteus and pseudomonas. it has low toxicity in laboratory animals. | 1976 | 956036 |