| effect of nitrogen limitation on long-side-chain poly-beta-hydroxyalkanoate synthesis by pseudomonas resinovorans. | pseudomonas resinovorans produced poly-beta-hydroxyalkanoates (phas) when grown on hydrocarbons but not on glucose. in a chemostat culture, the pha composition was beta-hydroxybutyrate (c4)-beta-hydroxyhexanoate (c6)-beta-hydroxyoctanoate (c8)-beta-hydroxydecanoate (c10) (1:15:75:9) on octanoate and c4-c6-c8-c10 (8:62:23:7) on hexanoic acid. contrary to the reported behavior of pseudomonas oleovorans, the pha accumulation rate increased under ammonium limitation on octanoate. | 1992 | 1610198 |
| radiation crosslinking of a bacterial medium-chain-length poly(hydroxyalkanoate) elastomer from tallow. | pseudomonas resinovorans produces a medium-chain-length poly(hydroxyalkanoate) (mcl-pha) copolymer when grown on tallow (pha-tal). this polymer had a repeat unit composition ranging from c4 to c14 with some mono-unsaturation in the c12 and c14 alkyl side chains. thermal analysis indicated that the polymer was semi-crystalline with a melting temperature (t(m)) of 43.5 +/- 0.2 degrees c and a glass transition temperature (tg) of -43.4 +/- 2.0 degrees c. the presence of unsaturated side chains allo ... | 1998 | 9644597 |
| glucose/lipid mixed substrates as a means of controlling the properties of medium chain length poly(hydroxyalkanoates). | glucose-triacylglycerol (tag) mixed substrates were used to modulate the physical and mechanical properties of medium-chain-length poly(hydroxyalkanoates) (mcl-phas). pseudomonas resinovorans nrrl b-2649 grew and produced mcl-phas on glucose and tags (coconut oil, c; soybean oil, s) after 24 h in a shake flask culture. however, with the exception of coconut oil, maximum cell productivity was not reached in any of the cultures until 72 h post-inoculation. here, 50:50 mixtures of glucose and cocon ... | 2001 | 11749175 |
| organization and transcriptional characterization of catechol degradation genes involved in carbazole degradation by pseudomonas resinovorans strain ca10. | pseudomonas resinovorans strain ca10 assimilates catechol, which is an intermediate of carbazole degradation, by ortho cleavage pathway enzymes encoded by the catr, catbca operon. cat proteins of strain ca10 were very similar to those of p. putida, although the relatedness in non-coding regions was not high. it was found that catbca genes were induced in carbazole-grown cells as a single transcriptional unit. | 2002 | 12036072 |
| sphingomonas sp. strain ka1, carrying a carbazole dioxygenase gene homologue, degrades chlorinated dibenzo-p-dioxins in soil. | hybridization analysis showed that a newly isolated carbazole (car)-degrading bacterium sphingomonas sp. strain ka1 did not possess the gene encoding the terminal oxygenase component (caraa) of car 1,9a-dioxygenase at high homology (more than 90% identity) to that of another car-degrader, pseudomonas resinovorans strain ca10. however, pcr experiments using the primers for amplifying the internal fragment of the caraa gene (810 bp for strain ca10) showed that a pcr product of unexpected size (110 ... | 2002 | 12052549 |
| crystallization and preliminary crystallographic analysis of the terminal oxygenase component of carbazole 1,9a-dioxygenase of pseudomonas resinovorans strain ca10. | the terminal oxygenase component (caraa) of carbazole 1,9a-dioxygenase from pseudomonas resinovorans strain ca10 was crystallized at 293 k using the sitting-drop vapour-diffusion method under the following conditions: 0.1 m sodium citrate ph 5.6 in the presence of 0.5 m ammonium sulfate and 1.0 m lithium sulfate. by using additive reagents with the crystallizing condition, improved diffraction was obtained from the crystals. preliminary x-ray diffraction analysis indicated that caraa crystals ar ... | 2002 | 12136152 |
| enhanced degradation of carbazole and 2,3-dichlorodibenzo-p-dioxin in soils by pseudomonas resinovorans strain ca10. | we studied the degradation of carbazole (car) and 2,3-dichlorodibenzo-p-dioxin (2,3-dcdd) in soils inoculated with carbazole- and dioxin-degrader pseudomonas resinovorans strain ca10. by using tn5-based transposon delivery systems, this bacterium was chromosomally marked with a tandem green fluorescent protein (gfp) gene. real-time competitive pcr and direct counting using the (gfp) marker were employed to monitor the total number of carbazole 1,9a-dioxygenase gene (caraa) and survival of ca10 c ... | 2002 | 12363321 |
| purification and characterization of carbazole 1,9a-dioxygenase, a three-component dioxygenase system of pseudomonas resinovorans strain ca10. | the carbazole 1,9a-dioxygenase (cardo) system of pseudomonas resinovorans strain ca10 consists of terminal oxygenase (caraa), ferredoxin (carac), and ferredoxin reductase (carad). each component of cardo was expressed in escherichia coli strain bl21(de3) as a native form (caraa) or a his-tagged form (carac and carad) and was purified to apparent homogeneity. caraa was found to be trimeric and to have one rieske type [2fe-2s] cluster and one mononuclear iron center in each monomer. both his-tagge ... | 2002 | 12450807 |
| bacterial degradation of aromatic compounds via angular dioxygenation. | dioxygenation is one of the important initial reactions of the bacterial degradation of various aromatic compounds. aromatic compounds, such as biphenyl, toluene, and naphthalene, are dioxygenated at lateral positions of the aromatic ring resulting in the formation of cis-dihydrodiol. this "normal" type of dioxygenation is termed lateral dioxygenation. on the other hand, the analysis of the bacterial degradation of fluorene (fn) analogues, such as 9-fluorenone, dibenzofuran (df), carbazole (car) ... | 2001 | 12483604 |
| complete nucleotide sequence of carbazole/dioxin-degrading plasmid pcar1 in pseudomonas resinovorans strain ca10 indicates its mosaicity and the presence of large catabolic transposon tn4676. | the car and ant operons originally isolated from pseudomonas resinovorans strain ca10 contain the genes encoding the carbazole/dioxin-degrading enzymes and anthranilate 1,2-dioxygenase, respectively, and are located on the plasmid pcar1. the entire nucleotide sequence of pcar1 was determined to elucidate the mechanism by which the car operon may have been assembled and distributed in nature. pcar1 is a 199,035-bp circular plasmid, and carries 190 open reading frames. although the incompatibility ... | 2003 | 12547188 |
| purification and characterization of meta-cleavage compound hydrolase from a carbazole degrader pseudomonas resinovorans strain ca10. | 2-hydroxy-6-oxo-6-(2'-aminophenyl)-hexa-2,4dienoic acid [6-(2'-aminophenyl)-hoda] hydrolase, involved in carbazole degradation by pseudomonas resinovorans strain ca10, was purified to near homogeneity from an overexpressing escherichia coli strain. the enzyme was dimeric, and its optimum ph was 7.0-7.5. phylogenetic analysis showed the close relationship of this enzyme to other hydrolases involved in the degradation of monocyclic aromatic compounds, and this enzyme was specific for 2-hydroxy-6-o ... | 2003 | 12619671 |
| crystal structure of a histidine-tagged serine hydrolase involved in the carbazole degradation (carc enzyme). | 2-hydroxy-6-oxo-6-(2(')-aminophenyl)-hexa-2,4-dienoate hydrolases (carc enzymes) from two carbazole-degrading bacteria were purified using recombinant escherichia coli strains with the histidine (his)-tagged purification system. the his-tagged carc (ht-carc) enzymes from pseudomonas resinovorans strain ca10 (ht-carc(ca10)) and janthinobacterium sp. strain j3 (ht-carc(j3)) exhibited hydrolase activity toward 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate as the purified native carc(ca10) did. ht-carc( ... | 2003 | 12659866 |
| expression, purification, and characterization of 2'-aminobiphenyl-2,3-diol 1,2-dioxygenase from carbazole-degrader pseudomonas resinovorans strain ca10. | the two-subunit meta-cleavage enzyme, 2'-aminobiphenyl-2,3-diol 1,2-dioxygenase (carbabb), from the carbazole degrader pseudomonas resinovorans strain ca10 was purified to homogeneity from an escherichia coli strain carrying the expression vector puca503, in which two copies of the carbabb genes are tandemly linked. sds-page and gel filtration showed that carb was a alpha2beta2-heterotetrameric enzyme with subunit molecular masses of approximately 10,000 for carba and 29,000 for carbb. the optim ... | 2003 | 12728990 |
| effect of inactivation of poly(hydroxyalkanoates) depolymerase gene on the properties of poly(hydroxyalkanoates) in pseudomonas resinovorans. | the phaz gene of pseudomonas resinovorans codes for a poly(hydroxyalkanoates) (pha) depolymerase. two phaz mutants of pseudomonas resinovorans nrrl b-2649, foac001 and foac002, were constructed by an in vitro transposition procedure followed by chromosomal integration via homologous recombination. a detailed mapping of the transposon insertion sites and an analysis of the resultant sequences showed that putative fusion polypeptides phaz(foac001) (239 amino-acid residues) and phaz(foac002) (85 am ... | 2003 | 12759786 |
| biosynthesis of medium-chain-length poly(hydroxyalkanoates) with altered composition by mutant hybrid pha synthases. | pseudomonas resinovorans harbors two isogenic poly(hydroxyalkanoates) (phas) synthase genes ( phac1(pre), phac2(pre)) responsible for the production of intracellular medium-chain-length (mcl-)phas. sequence analysis showed that the putative gene-products of these genes contain a conserved alpha/beta-hydrolase fold in the carboxy-terminal half of the proteins. hybrid genes pha7 and pha8 were constructed by exchanging the alpha/beta-hydrolase-fold coding portions of phac1(pre) and phac2(pre) at th ... | 2003 | 12761653 |
| [a soil bacterium capable of utilizing, as a source of carbon, the fixed fraction of certain oleoresins. pseudomonas resinovorans n. sp]. | | 1961 | 13721465 |
| [utilization of malonic acid as a source of carbon by pseudomonas resinovorans]. | | 1962 | 14490732 |
| divergent structures of carbazole degradative car operons isolated from gram-negative bacteria. | southern hybridization analysis of the genomes from the newly-isolated 10 carbazole (car)-utilizing bacteria revealed that 8 of the isolates carried gene clusters homologous to the car-catabolic car operon of pseudomonas resinovorans strain ca10. sequencing analysis showed that two car operons and the neighboring regions of pseudomonas sp. strain k23 are nearly identical to that of strain ca10. in contrast to strains ca10 and k23, caref genes did not exist downstream of the car gene cluster of j ... | 2004 | 15277751 |
| transcriptional regulation of the ant operon, encoding two-component anthranilate 1,2-dioxygenase, on the carbazole-degradative plasmid pcar1 of pseudomonas resinovorans strain ca10. | the carbazole-degradative plasmid pcar1 of pseudomonas resinovorans strain ca10 has two gene clusters, caraaaababbcacaddfe and antabc, which are involved in the conversions of carbazole to anthranilate and anthranilate to catechol, respectively. we proved that the antabc gene cluster, encoding two-component anthranilate 1,2-dioxygenase, constitutes a single transcriptional unit through northern hybridization and reverse transcription-pcr (rt-pcr) analyses. the transcription start point of anta w ... | 2004 | 15466034 |
| crystallization and preliminary crystallographic analysis of the 2'-aminobiphenyl-2,3-diol 1,2-dioxygenase from the carbazole-degrader pseudomonas resinovorans strain ca10. | carbabb, the class iii extradiol dioxygenase involved in carbazole degradation by pseudomonas resinovorans ca10, was crystallized at 278 k by the hanging-drop vapour-diffusion method using peg mme 550 as a precipitant. the crystals had a transparent thin square-pillar shape and belonged to space group p2(1)2(1)2, with unit-cell parameters a = 122.8, b = 144.6, c = 49.2 a, alpha = beta = gamma = 90 degrees . the crystals diffracted to a maximum resolution of 1.9 a and gave a data set with an over ... | 2004 | 15583384 |
| crystal structure of the ferredoxin component of carbazole 1,9a-dioxygenase of pseudomonas resinovorans strain ca10, a novel rieske non-heme iron oxygenase system. | the carbazole 1,9a-dioxygenase (cardo) system of pseudomonas resinovorans strain ca10 catalyzes the dioxygenation of carbazole; the 9ac carbon bonds to a nitrogen atom and its adjacent 1c carbon as the initial reaction in the mineralization pathway. the cardo system is composed of ferredoxin reductase (carad), ferredoxin (carac), and terminal oxygenase (caraa). carac acts as a mediator in the electron transfer from carad to caraa. to understand the structural basis of the protein-protein interac ... | 2005 | 15645447 |
| diversity of carbazole-degrading bacteria having the car gene cluster: isolation of a novel gram-positive carbazole-degrading bacterium. | twenty-seven carbazole-utilizing bacterial strains were isolated from environmental samples, and were classified into 14 groups by amplified ribosomal dna restriction analysis. southern hybridization analyses showed that 3 and 17 isolates possessed the car gene homologs of pseudomonas resinovorans ca10 and sphingomonas sp. strain ka1, respectively. of the 17 isolates, 2 isolates also have the homolog of the caraa gene of sphingomonas sp. strain cb3. while the genome of one isolate, a gram-positi ... | 2005 | 15796992 |
| large plasmid pcar2 and class ii transposon tn4676 are functional mobile genetic elements to distribute the carbazole/dioxin-degradative car gene cluster in different bacteria. | the carbazole-catabolic plasmid pcar1 isolated from pseudomonas resinovorans strain ca10 was sequenced in its entirety; and it was found that pcar1 carries the class ii transposon tn4676 containing carbazole-degradative genes. in this study, a new plasmid designated pcar2 was isolated from p. putida strain hs01 that was a transconjugant from mating between the carbazole-degrader pseudomonas sp. strain k23 and p. putida strain ds1. southern hybridization and nucleotide sequence analysis of pcar1 ... | 2004 | 15856217 |
| regulation of pyrimidine synthesis in pseudomonas resinovorans. | to investigate the regulation of de novo pyrimidine biosynthesis in the bacterium pseudomonas resinovorans atcc 14235. | 2005 | 15892745 |
| differentiation of carbazole catabolic operons by replacement of the regulated promoter via transposition of an insertion sequence. | the carbazole catabolic car operons from pseudomonas resinovorans ca10 and janthinobacterium sp. j3 have nearly identical nucleotide sequences in their structural and intergenic regions but not in their flanking regions. transposition of ispre1 from the anthranilate catabolic ant operon located an inducible promoter pant upstream of the carca10 operon, which is regulated by the arac/xyls family activator antr in response to anthranilate. the transposed pant drives transcription of the carca10 op ... | 2006 | 16455652 |
| carbazole biodegradation in gas oil/water biphasic media by a new isolated bacterium burkholderia sp. strain imp5gc. | to select carbazole-degrading bacteria able to survive and metabolize carbazole in biphasic organic-water media and to study the factors affecting carbazole degradation in such conditions. | 2006 | 16553728 |
| plasmid pcar3 contains multiple gene sets involved in the conversion of carbazole to anthranilate. | the carbazole degradative car-i gene cluster (caraaibaibbiciaci) of sphingomonas sp. strain ka1 is located on the 254-kb circular plasmid pcar3. carbazole conversion to anthranilate is catalyzed by carbazole 1,9a-dioxygenase (cardo; caraaiaci), meta-cleavage enzyme (carbaibbi), and hydrolase (carci). cardo is a three-component dioxygenase, and caraai and caraci are its terminal oxygenase and ferredoxin components. the car-i gene cluster lacks the gene encoding the ferredoxin reductase component ... | 2006 | 16672458 |
| characterization of the replication, maintenance, and transfer features of the incp-7 plasmid pcar1, which carries genes involved in carbazole and dioxin degradation. | isolated from pseudomonas resinovorans ca10, pcar1 is a 199-kb plasmid that carries genes involved in the degradation of carbazole and dioxin. the nucleotide sequence of pcar1 has been determined previously. in this study, we characterized pcar1 in terms of its replication, maintenance, and conjugation. by constructing miniplasmids of pcar1 and testing their establishment in pseudomonas putida ds1, we show that pcar1 replication is due to the repa gene and its upstream dna region. the repa gene ... | 2006 | 16672459 |
| characterization of novel carbazole catabolism genes from gram-positive carbazole degrader nocardioides aromaticivorans ic177. | nocardioides aromaticivorans ic177 is a gram-positive carbazole degrader. the genes encoding carbazole degradation (car genes) were cloned into a cosmid clone and sequenced partially to reveal 19 open reading frames. the car genes were clustered into the caraacbabbacad and cardfe gene clusters, encoding the enzymes responsible for the degradation of carbazole to anthranilate and 2-hydroxypenta-2,4-dienoate and of 2-hydroxypenta-2,4-dienoate to pyruvic acid and acetyl coenzyme a, respectively. th ... | 2006 | 16672473 |
| transcriptome analysis of pseudomonas putida kt2440 harboring the completely sequenced incp-7 plasmid pcar1. | the incp-7 plasmid pcar1 of pseudomonas resinovorans ca10 confers the ability to degrade carbazole upon transfer to the recipient strain p. putida kt2440. we designed a customized whole-genome oligonucleotide microarray to study the coordinated expression of pcar1 and the chromosome in the transconjugant strain kt2440(pcar1). first, the transcriptome of kt2440(pcar1) during growth with carbazole as the sole carbon source was compared to that during growth with succinate. the carbazole catabolic ... | 2007 | 17675379 |
| competition between two microbial populations in a sequencing fed-batch reactor: theory, experimental verification, and implications for waste treatment applications. | competition between two microbial populations for a single pollutant (phenol) was studied in a sequencing fed-batch reactor (sfbr). a mathematical model describing this system was developed and tested experimentally. it is based on specific growth rate expressions revealed from pure culture batch experiments. the species employed were pseudomonas putida (atcc 17514) and pseudomonas resinovorans (atcc 14235). it was found that both species biodegrade phenol following inhibitory kinetics which can ... | 1993 | 18613087 |
| high-resolution mapping of plasmid transcriptomes in different host bacteria. | plasmids are extrachromosomal elements that replicate autonomously, and many can be transmitted between bacterial cells through conjugation. although the transcription pattern of genes on a plasmid can be altered by a change in host background, the expression range of plasmid genes that will result in phenotypic variation has not been quantitatively investigated. | 2009 | 19134166 |
| novel marine carbazole-degrading bacteria. | eleven carbazole (car)-degrading bacterial strains were isolated from seawater collected off the coast of japan using two different media. seven isolates were shown to be most closely related to the genera erythrobacter, hyphomonas, sphingosinicella, caulobacter, and lysobacter. meanwhile, strains oc3, oc6s, oc9, and oc11s showed low similarity to known bacteria, the closest relative being kordiimonas gwangyangensis gw14-5 (90% similarity). southern hybridization analysis revealed that only five ... | 2009 | 19187209 |
| isolation and characterization of a car gene cluster from the naphthalene, phenanthrene, and carbazole-degrading marine isolate lysobacter sp. strain oc7. | the novel carbazole (car)-degrading bacterium lysobacter sp. strain oc7 has been isolated from seawater and can also utilize naphthalene and phenanthrene as its sole carbon and energy source. the car-degradative gene cluster was isolated and encoded five complete open reading frames (orfs) and two truncated orfs. among them, four orfs showed 40-50% similarity with previously reported car-degradative genes. ferredoxin (carac) and ferredoxin reductase (carad) genes, which are necessary for the car ... | 2009 | 19452212 |
| methyl-branched poly(hydroxyalkanoate) biosynthesis from 13-methyltetradecanoic acid and mixed isostearic acid isomer substrates. | pseudomonas resinovorans, a known medium-chain-length (mcl-) poly(hydroxyalkanoate) (pha) producer, was grown on 13-methyltetradecanoic acid (13-mtda) and a mixture of isostearic acid (ia) isomers to produce methyl-branched mcl-pha polymers. shake-flask experiments revealed polymer productivities (the percent of the cell mass that is polymer) of 31 +/- 1% (n = 3) and 23 +/- 3% (n = 3) when grown in 13-mtda and ia, respectively. monomer content was determined by a combination of gas chromatograph ... | 2009 | 19644690 |
| isolation of novel pseudomonas syringae promoters and functional characterization in polyhydroxyalkanoate-producing pseudomonads. | a library of genomic dna fragments of pseudomonas syringae pv. tomato dc3000 was constructed in a laczalpha-containing plasmid, pbs29. the library was used in a preliminary alpha-complementation-based screen to identify clones with promoter activity in escherichia coli. ten positive clones were sequenced and their locations in the chromosomal dna of dc3000 strain were mapped. five positive clones (p2, p3, p4, p6 and p8) were further assayed for promoter activity in three polyhydroxyalkanoate-pro ... | 2010 | 20034598 |
| tailor-made type ii pseudomonas pha synthases and their use for the biosynthesis of polylactic acid and its copolymer in recombinant escherichia coli. | previously, we have developed metabolically engineered escherichia coli strains capable of producing polylactic acid (pla) and poly(3-hydroxybutyrate-co-lactate) [p(3hb-co-la)] by employing evolved clostridium propionicum propionate coa transferase (pct(cp)) and pseudomonas sp. mbel 6-19 polyhydroxyalkanoate (pha) synthase 1 (phac1(ps6-19)). introduction of mutations four sites (e130, s325, s477, and q481) of phac1( ps6-19) have been found to affect the polymer content, lactate mole fraction, an ... | 2011 | 21221571 |
| pseudomonas resinovorans spr1, a newly isolated strain with potential of transforming eugenol to vanillin and vanillic acid. | in this study a novel strain was isolated with the capability to grow on eugenol as a source of carbon and energy. this strain was identified as pseudomonas resinovorans (genbank accession no. hq198585) based on phenotypic characterization and phylogenetic analysis of 16s rdna gene. the intermediates coniferyl alcohol, coniferyl aldehyde, ferulic acid, vanillin and vanillic acid were detected in the culture supernatant during eugenol biotransformation with this strain. the products were confirme ... | 2011 | 21689800 |
| manipulation of förster energy transfer of coupled fluorophores through biotransformation by pseudomonas resinovorans ca10. | an alkyne-terminated anthracene and azide-terminated carbazole were joined through a copper-catalyzed cycloaddition to form a joined donor/acceptor pair. the photonic pair exhibited energy transfer when excited at the peak absorbance of carbazole and fluoresced with an anthracene spectral response. the fluorescent behavior was confirmed as förster energy transfer (fret). the lysate of pseudomonas resinovorans ca10, a member of a predominant group of soil microorganisms that can metabolize a host ... | 2011 | 22044050 |
| high-rate biological denitrification in the cyclic rotating-bed biological reactor: effect of cod/no3(-), nitrate concentration and salinity and the phylogenetic analysis of denitrifiers. | the effects of cod/no3(-) ratio, nitrate concentration and salinity was tested on the performance of the crbr in denitrification with catechol as carbon source. the maximum nitrate reduction attained at cod/no3(-) ratio of 1. the crbr operated at optimum cod/no3(-) ratio could completely denitrify the nitrate at inlet concentration up to 1250mg/l without nitrite accumulation. the maximum denitrification rate in the crbr was 3.56kgno3(-)/m(3)d with a nitrate reduction efficiency of 99% when the b ... | 2015 | 26369277 |
| valorization of fatty acids-containing wastes and byproducts into short- and medium-chain length polyhydroxyalkanoates. | olive oil distillate (ood), biodiesel fatty acids-byproduct (fab) and used cooking oil (uco) were tested as inexpensive carbon sources for the production of polyhydroxyalkanoates (pha) with different composition using twelve bacterial strains. ood and fab were exploited for the first time as alternative substrates for pha production. uco, ood and fab were used by cupriavidus necator and pseudomonas oleovorans to synthesize the homopolymer poly-3-hydroxybutyrate, while pseudomonas resinovorans an ... | 2016 | 26047553 |
| the behavior of mobile genetic elements (mges) in different environments. | mobile genetic elements (mges) including plasmids have an important role in the rapid evolution and adaptation of bacteria. here, the behavior of mges in different environments is reviewed, in particular, behavior of the plasmid pcar1, a carbazole-degradative plasmid isolated from pseudomonas resinovorans ca10. pcar1 belongs to incompatibility p-7 group and is self-transmissible among different bacteria. comparisons of changes in the transcriptome of different host strains caused by carrying pca ... | 2017 | 28077029 |
| characterization of medium chain length polyhydroxyalkanoate produced from olive oil deodorizer distillate. | olive oil deodorizer distillate (oodd) was used for the first time as the sole substrate for polyhydroxyalkanoates (pha) production by the bacterium pseudomonas resinovorans in bioreactor cultivation. a pha content in the biomass of 36 ± 0.8 wt% was attained within 19 h of cultivation. a final polymer concentration of 4.7 ± 0.3 gl(-1) was reached, corresponding to a volumetric productivity of 5.9 ± 0.2 gl(-1)day(-1). the pha was composed of 3-hydroxyoctanoate (48.3 ± 7.3 mol%), 3-hydroxydecanoat ... | 2016 | 26484598 |
| fruit pomace and waste frying oil as sustainable resources for the bioproduction of medium-chain-length polyhydroxyalkanoates. | medium-chain-length polyhydroxyalkanoates (mcl-phas) are biobased and biodegradable alternatives to petrol-derived polymers, whose break-through has been prevented by high production cost. therefore we investigated whether wastes from the food industry (nine types of fruit pomace including apricots, cherries and grapes, and waste frying oil) could replace the costly sugars and fatty acids typically used as carbon substrates for the bacterial fermentations. a selection of enzyme preparations was ... | 2014 | 24882726 |
| complete genome sequence of the carbazole degrader pseudomonas resinovorans strain ca10 (nbrc 106553). | pseudomonas resinovorans strain ca10 can grow on carbazole as its sole carbon and nitrogen source. here, we report the complete nucleotide sequence of the ca10 genome (a 6,285,863-bp chromosome and a 198,965-bp plasmid). ca10 carries a larger number of genes that are potentially responsible for aromatic hydrocarbon metabolism than do other previously sequenced pseudomonas spp. | 2013 | 23887915 |
| cloning, sequencing, and characterization of lipase genes from a polyhydroxyalkanoate (pha)-synthesizing pseudomonas resinovorans. | lipase (lip) and lipase-specific foldase (lif) genes of a biodegradable polyhydroxyalkanoate (pha)-synthesizing pseudomonas resinovorans nrrl b-2649 were cloned using primers based on consensus sequences, followed by polymerase chain reaction-based genome walking. sequence analyses showed a putative lip gene product (314 amino acids, a.a.) with its catalytic active site (ser(111), asp(258), and his(280)) identified. the foldase lif gene that is located 55 bp downstream of lip codes for a putativ ... | 2012 | 22644524 |
| structural and molecular genetic analyses of the bacterial carbazole degradation system. | carbazole degradation by several bacterial strains, including pseudomonas resinovorans ca10, has been investigated over the last two decades. as the initial reaction in degradation pathways, carbazole is commonly oxygenated at angular (c9a) and adjacent (c1) carbons as two hydroxyl groups in a cis configuration. this type of dioxygenation is termed "angular dioxygenation," and is catalyzed by carbazole 1,9a-dioxygenase (cardo), consisting of terminal oxygenase, ferredoxin, and ferredoxin reducta ... | 2012 | 22232235 |
| diversity of culturable aerobic denitrifying bacteria in the sediment, water and biofilms in liangshui river of beijing, china. | aerobic denitrification is a process reducing the nitrate into gaseous nitrogen forms in the presence of oxygen gas, which makes the nitrification and denitrification performed simultaneously. however, little was known on the diversity of the culturable aerobic denitrifying bacteria in the surface water system. in this study, 116 strains of aerobic denitrifying bacteria were isolated from the sediment, water and biofilm samples in liangshui river of beijing. these bacteria were classified into 1 ... | 2017 | 28855587 |