| monoclonal antibodies against pseudomonas aeruginosa outer membrane antigens: isolation and characterization. | hybridomas secreting monoclonal antibodies specific for pseudomonas aeruginosa outer membrane antigens were isolated. one of the antibodies was highly specific for the o antigen of the lipopolysaccharide of international antigen typing scheme serotype 5 strains, reacting only weakly with a serotype 17 strain and failing to react with the outer membranes of strains representing 15 other serotypes. this monoclonal antibody was able to agglutinate heat-killed bacterial cells as well as lipopolysacc ... | 1982 | 6809625 |
| isolation of cyanophycin-degrading bacteria, cloning and characterization of an extracellular cyanophycinase gene (cphe) from pseudomonas anguilliseptica strain bi. the cphe gene from p. anguilliseptica bi encodes a cyanophycinhydrolyzing enzyme. | eleven bacteria capable of utilizing cyanophycin (cyanophycin granule polypeptide (cgp)) as a carbon source for growth were isolated. one isolate was taxonomically affiliated as pseudomonas anguilliseptica strain bi, and the extracellular cyanophycinase (cphe) was studied because utilization of cyanophycin as a carbon source and extracellular cyanophycinases were hitherto not described. cphe was detected in supernatants of cgp cultures and purified from a corresponding culture of strain bi emplo ... | 2002 | 11986309 |
| pcr detection and pfge dna macrorestriction analyses of clinical isolates of pseudomonas anguilliseptica from winter disease outbreaks in sea bream sparus aurata. | a pcr-based detection system for pseudomonas anguilliseptica was evaluated. the primer combination paf-par (forward primer paf = 5'-gacctcgccatta-3', reverse primer par = 5'-ctcagcagttttgaaag-3') gave a unique and specific amplification product of 439 bp at an annealing temperature of 46 degrees c with all the p. anguilliseptica isolates and strains (n = 56) but no amplification products were observed with any other pseudomonas species or phylogenetically related bacteria tested. the pcr assay h ... | 2002 | 12152901 |
| existence of two o-serotypes in the fish pathogen pseudomonas anguilliseptica. | the serological characteristics of a group of 32 pseudomonas anguilliseptica strains isolated in spain from seabream (sparus aurata) and turbot (scophthalmus maximus) were compared with a total of 18 collection strains of this bacterial species with different geographical and host origin. the employment of different techniques, including slide agglutination, microagglutination and dot blot, allowed us to establish two serological groups, one comprising practically all the eel isolates, and the o ... | 2003 | 12829386 |
| freshwater bacteria can methylate selenium through the thiopurine methyltransferase pathway. | involvement of the bacterial thiopurine methyltransferase (btpmt) in natural selenium methylation by freshwater was investigated. a freshwater environment that had no known selenium contamination but exhibited reproducible emission of dimethyl selenide (dmse) or dimethyl diselenide (dmdse) when it was supplemented with an organic form of selenium [(methyl)selenocysteine] or an inorganic form of selenium (sodium selenite) was used. the distribution of the btpmt gene (tpm) in the microflora was st ... | 2003 | 12839745 |
| isolation and characterization of gram-positive cyanophycin-degrading bacteria-kinetic studies on cyanophycin depolymerase activity in aerobic bacteria. | this study is the first report on the extracellular degradation of cyanophycin (cgp) by gram-positive bacteria. three different gram-positive bacteria were isolated from forest soil that were able to utilize cgp as the sole carbon source for growth. the isolates were assigned to species of the genera bacillus and micromonospora. from one of the isolates, which was taxonomically affiliated as bacillus megaterium strain bac19, the extracellular cgp depolymerase (extracellular cgpase; cphebm) was p ... | 2004 | 14715021 |
| pseudomonas anguilliseptica infection in farmed cod, gadus morhua l. | | 2004 | 15049894 |
| vaccination strategies to prevent emerging diseases for spanish aquaculture. | in recent years, three serious diseases have emerged in spanish aquaculture. these are lactococcosis caused by lactococcus garvieae, which is of economical importance in rainbow trout (oncorhynchus mykiss); pseudomonadiasis caused by pseudomonas anguilliseptica which affects gilthead seabream (sparus aurata) and turbot (scophthalmus maximus); and flexibacteriosis caused by tenacibaculum maritimum which became a devastating problem in the emerging culture of sole (solea spp). to obtain useful inf ... | 2005 | 15962472 |
| phenotypic, serological and genetic characterization of pseudomonas anguilliseptica strains isolated from cod, gadus morhua l., in northern europe. | the biochemical, serological and genetic characteristics of six strains of pseudomonas anguilliseptica isolated from cod, gadus morhua, in scotland were compared to well characterized isolates of this same bacterial species but of different origin. biochemical and physiological analyses showed that this group of isolates was highly homogeneous, their characteristics matching previous descriptions of the pathogen. similar results were obtained for the six cod isolates in the serological assays, a ... | 2007 | 17958609 |
| pseudomonas guineae sp. nov., a novel psychrotolerant bacterium from an antarctic environment. | two gram-negative, cold-adapted, aerobic bacteria, designated strains m8t and m6, were isolated from soil collected from the south shetland islands. the organisms were rod-shaped, catalase- and oxidase-positive and motile by means of polar flagella. these two psychrotolerant strains grew between -4 and 30 degrees c. 16s rrna gene sequence analysis placed strains m8t and m6 within the genus pseudomonas. dna-dna hybridization experiments between the antarctic isolate m8t and type strains of phylog ... | 2007 | 17978227 |
| polymerase chain reaction amplification of repetitive intergenic consensus and repetitive extragenic palindromic sequences for molecular typing of pseudomonas anguilliseptica and aeromonas salmonicida. | the aim of this study was to evaluate the use of two molecular techniques, repetitive extragenic palindromic polymerase chain reaction (rep-pcr) and repetitive intergenic consensus pcr (eric-pcr), as epidemiological tools with which to discriminate among genetically distinct strains within two bacterial fish pathogens, pseudomonas anguilliseptica and aeromonas salmonicida. a total of 30 a. salmonicida and 52 p. anguilliseptica were analyzed. for p. anguilliseptica, three different major fingerpr ... | 2008 | 18783128 |
| experimental pseudomonas anguilliseptica infection in turbot psetta maxima (l.): a histopathological and immunohistochemical study. | experimental infection with pseudomonas anguilliseptica was performed both by intraperitoneal (i.p.) and bath route on juvenile turbot (psetta maxima) in order to evaluate the pathology induced. turbot was found to be sensitive to i.p. challenge (1.7x10(6) cfu/fish) but no to bath exposure. the i.p. challenge induced septicaemic infection and mortality. externally, moribund fish showed distended abdomen and pale areas at day 9. the gross pathological internal signs present were abundant ascitic ... | 2009 | 19683980 |
| bacterial pathogens and flora isolated from farm-cultured eels (anguilla japonica) and their environmental waters in korean eel farms. | the purpose of this study was to investigate bacterial pathogens and flora in both sick and clinically healthy eels, anguilla japonica, and the environmental rearing waters of korean eel farms. between 2003 and 2010, a total of 621 sick eels were submitted for diagnosis, while 216 healthy eels and 87 environmental water samples were collected during a survey of 26 eel farms in korea. seven different bacterial species were obtained from 183 isolates, which were recovered from the internal organs ... | 2012 | 23357049 |
| simultaneous detection of multiple fish pathogens using a naked-eye readable dna microarray. | we coupled 16s rdna pcr and dna hybridization technology to construct a microarray for simultaneous detection and discrimination of eight fish pathogens (aeromonas hydrophila, edwardsiella tarda, flavobacterium columnare, lactococcus garvieae, photobacterium damselae, pseudomonas anguilliseptica, streptococcus iniae and vibrio anguillarum) commonly encountered in aquaculture. the array comprised short oligonucleotide probes (30 mer) complementary to the polymorphic regions of 16s rrna genes for ... | 2012 | 22736973 |
| co-infection with pseudomonas anguilliseptica and delftia acidovorans in the european eel, anguilla anguilla (l.): a case history of an illegally trafficked protected species. | inspections by customs agents at barcelona airport discovered 420 kg of contraband glass eels prepared for shipment to hong kong. after confiscation of these animals by police, they were transported to holding facilities to be maintained until after a judicial hearing. upon arrival, they were separated into two groups and held under ambient flow-through conditions in fresh water. during their captivity period, several peaks in mortality occurred and multiple bacterial strains were isolated from ... | 2013 | 23297744 |
| amoebic gill disease outbreak in marine fish cultured in korea. | in 2015, 6.7-60% mortality was observed in black seabream ( acanthopagrus schlegelii), rock bream ( oplegnathus fasciatus), and gray mullet ( mugil cephalus) farmed in the southern coast of korea. on examination, numerous amoebae were found on the gills of these 3 fish species with detection rate of 100%. some rock bream and gray mullet were coinfected with bacteria ( pseudomonas anguilliseptica, vibrio tapetis, or vibrio anguillarum). histologic examination revealed extensive hyperplastic epith ... | 2017 | 28205457 |
| eutrophication, ammonia intoxication, and infectious diseases: interdisciplinary factors of mass mortalities in cultured nile tilapia. | the present study was designed to assess the possible causes of the mass mortalities of nile tilapia oreochromis niloticus at el-behera governorate, egypt, in relationship to environmental and microbiotic factors. water samples were collected from fish farms at different locations and from lake edku to analyze water temperature, water ph, salinity, biological oxygen demand, dissolved oxygen, total ammonia nitrogen, and un-ionized ammonia. a number of moribund and freshly dead fish were sampled a ... | 2016 | 27484819 |
| aflatoxin b1 degradation by liquid cultures and lysates of three bacterial strains. | aflatoxin contamination remains a daunting issue to address in food safety. in spite of the efforts geared towards prevention and elimination of this toxin, it still persists in agricultural commodities. this has necessitated the search for other measures such as microbial degradation to combat this hazard. in this study, we investigated the biodegradation of aflatoxin b1 (afb1), using lysates of three bacterial strains (pseudomonas anguilliseptica vgf1, pseudomonas fluorescens and staphylococcu ... | 2016 | 27294556 |
| efficacy of formalin-killed pseudomonas anguilliseptica vaccine on immune gene expression and protection in farmed olive flounder, paralichthys olivaceus. | formalin killed pseudomonas anguilliseptica bacterial vaccine was prepared and administered to farm reared olive flounder, paralichthys olivaceus reared at 17 °c and 20 °c for 4 weeks. non-vaccinated fishes (n=150) served as positive control. vaccinated fishes were divided into two groups (n=150 each in replicate). both the vaccinated and non-vaccinated fishes were challenged intraperitoneally with p. anguilliseptica (1×10(7) cfu ml(-1)) isolates and pbs (negative control). fishes were sampled f ... | 2014 | 24530933 |
| oxasetin from lophiostoma sp. of the baltic sea: identification, in silico binding mode prediction and antibacterial evaluation against fish pathogenic bacteria. | because of the evolving resistance of microorganisms against existing antibiotics, there is an increasing need for new ones, not only in human, but also in veterinary medicine. the dichloromethane extract of a fungal strain of the genus lophiostoma, isolated from driftwood collected from the coast of the baltic sea, displayed antibacterial activity against some fish pathogenic bacteria. ergosterol epoxide (1), cerebroside c (2) and oxasetin (3) were isolated from the extract and structurally elu ... | 2013 | 24273851 |