| isolation of enterococcus mundtii from normally sterile body sites in two patients. | enterococcus mundtii, a recently described nonmotile, yellow-pigmented enterococcal species, was isolated from a chronic thigh abscess and from operatively obtained sinus mucosa. it is emphasized that this species may be encountered in clinical specimens and that the correct species identification may be missed when commercially available identification systems are relied on. | 1991 | 2056046 |
| resistance of enterococcus faecium to neutrophil-mediated phagocytosis. | during a previous study of the opsonic requirements for neutrophil (polymorphonuclear leukocyte [pmn])-mediated killing of enterococci, we identified two strains of enterococcus faecium (tx0015 and tx0016) that were resistant to pmn-mediated killing. to better define the mechanism of this resistance, we examined phagocytosis with a fluorescence assay and found that tx0016 was completely resistant to phagocytosis by pmns; this finding was confirmed by electron microscopy. examination of multiple ... | 1994 | 7960141 |
| presumptive fecal streptococci in environmental samples characterized by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis. | the use of fecal streptococci as fecal indicators requires better knowledge of the ecology of these bacteria. we isolated 371 presumptive fecal streptococci from environmental samples--domestic wastewater, forest industry wastewater, contaminated surface and seawater, well water, cow dung, bird droppings, and pristine waters--and clustered them according to their protein profiles in one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis. some clusters could be tentati ... | 1993 | 8357252 |
| primary and secondary structures of rrna spacer regions in enterococci. | the 16s-23s and 23s-5s rrna spacer dna regions (spacer regions 1 and 2, respectively) from enterococcus faecalis, enterococcus faecium, enterococcus hirae, enterococcus durans and enterococcus mundtii were amplified by pcr. their nucleotide sequences were established and a secondary structure model showing the interaction between the two spacer regions was built. whereas lactococci and streptococcus sensu stricto are characterized by a single type of spacer region 1, the enterococci show a high ... | 1997 | 9084166 |
| species identification of enterococci via intergenic ribosomal pcr. | accurate species identification of enterococci has become important with the wide prevalence of acquired vancomycin resistance and the presence of less epidemiologically important, inherently vancomycin-resistant enterococci. using a collection of enterococcal strains, we found that pcr amplification of the intergenic spacer (its-pcr) between the 16s and 23s rrna genes can produce amplicon profiles characteristic of the enterococcus examined. the species examined were group i enterococci (entero ... | 1997 | 9114380 |
| effects of different test conditions on mics of food animal growth-promoting antibacterial agents for enterococci. | the influence of the addition of sheep blood to mueller-hinton ii agar and the effects of aerobic incubation with or without co2 and of anaerobic incubation were tested with bacitracin, tylosin, avoparcin, virginiamycin, avilamycin, narasin, and flavomycin on enterococci. the antibacterial activity of bambermycin (flavomycin) was strongly inhibited by the addition of blood, except with the species enterococcus faecium, enterococcus mundtii, enterococcus hirae, enterococcus casseliflavus, and ent ... | 1998 | 9650934 |
| a novel bacteriocin with a ygngv motif from vegetable-associated enterococcus mundtii: full characterization and interaction with target organisms. | a novel broad-spectrum antimicrobial peptide produced by vegetable-associated enterococcus mundtii was purified and characterized, and designated mundticin. to our knowledge, this is the first report on bacteriocin production by this organism. the elucidation of the full primary amino acid sequence of mundticin (kyygngvscnkkgcsvdwgkaigiignnsaanlatggaagwsk) revealed that this antimicrobial peptide belongs to the class iia bacteriocins of lactic acid bacteria which share a highly conserved n-termi ... | 1998 | 9733915 |
| colonization and microbiology of the motile enterococci in a patient population. | the motile enterococci with the vanc gene have intrinsic low-level resistance to vancomycin, but have not been implicated in a nosocomial outbreak. we determined the colonization rate of motile enterococci in hospitalized and nonhospitalized patients. perianal or stool specimens were cultured in enterococcosel broth supplemented with 6 micrograms of vancomycin per ml. rapid motility and pigment tests were performed on all enterococci isolated. a total of 82 motile and/or pigmented enterococci we ... | 1998 | 9764390 |
| use of yellow-pigmented enterococci as a specific indicator of human and nonhuman sources of faecal pollution. | antibiotic susceptibility tests and restriction enzyme analysis (rea) of genomic dna were performed to characterize the relationship between sources of isolates of yellow-pigmented enterococci. antibiotic susceptibility tests were conducted with 10 therapeutic antibiotics and 54 isolates grouped by source (wild and other) depending on their origin. in three antibiotics, cephalothin, erythromycin, and vancomycin, there was a significant (p < or = 0.05) association between susceptibility and sourc ... | 1998 | 10030000 |
| biopreservation in modified atmosphere stored mungbean sprouts: the use of vegetable-associated bacteriocinogenic lactic acid bacteria to control the growth of listeria monocytogenes. | two bacteriocinogenic strains of pediococcus parvulus and one bacteriocinogenic enterococcus mundtii strain were evaluated for their potential to control the growth of listeria monocytogenes on refrigerated, modified atmosphere (ma) stored mungbean sprouts. these three strains, which were isolated from minimally-processed vegetables, were shown to grow in culture broth at 4, 8, 15 and 30 degrees c. however, only ent. mundtii was capable of bacteriocin production at 4-8 degrees c. examination of ... | 1999 | 10196774 |
| sequencing the gene encoding manganese-dependent superoxide dismutase for rapid species identification of enterococci. | simple pcr and sequencing assays that utilize a single pair of degenerate primers were used to characterize a 438-bp-long dna fragment internal (soda(int)) to the soda gene encoding the manganese-dependent superoxide dismutase in 19 enterococcal type strains (enterococcus avium, enterococcus casseliflavus, enterococcus cecorum, enterococcus columbae, enterococcus dispar, enterococcus durans, enterococcus faecalis, enterococcus faecium, enterococcus flavescens, enterococcus gallinarum, enterococc ... | 2000 | 10618129 |
| identification of enterococcus species and phenotypically similar lactococcus and vagococcus species by reverse checkerboard hybridization to chaperonin 60 gene sequences. | data from four recent studies (s. h. goh et al., j. clin. microbiol. 36:2164-2166, 1998; s. h. goh et al., j. clin. microbiol. 34:818-823, 1996; s. h. goh et al., j. clin. microbiol. 35:3116-3121, 1997; a. y. c. kwok et al., int. j. syst. bacteriol. 49:1181-1192, 1999) suggest that an approximately 600-bp region of the chaperonin 60 (cpn60) gene, amplified by pcr with a single pair of degenerate primers, has utility as a potentially universal target for bacterial identification (id). this cpn60 ... | 2000 | 11060051 |
| diversity of domain v of 23s rrna gene sequence in different enterococcus species. | the highly conserved central loop of domain v of 23s rna (nucleotides 2042 to 2628; escherichia coli numbering) is implicated in peptidyltransferase activity and represents one of the target sites for macrolide, lincosamide, and streptogramin b antibiotics. dna encoding domain v (590 bp) of several species of enterococcus was amplified by pcr. twenty enterococcal isolates were tested, including enterococcus faecium (six isolates), enterococcus faecalis, enterococcus avium, enterococcus durans, e ... | 2000 | 11060057 |
| evidence for horizontal gene transfer in evolution of elongation factor tu in enterococci. | the elongation factor tu, encoded by tuf genes, is a gtp binding protein that plays a central role in protein synthesis. one to three tuf genes per genome are present, depending on the bacterial species. most low-g+c-content gram-positive bacteria carry only one tuf gene. we have designed degenerate pcr primers derived from consensus sequences of the tuf gene to amplify partial tuf sequences from 17 enterococcal species and other phylogenetically related species. the amplified dna fragments were ... | 2000 | 11092850 |
| identification of enterococcal isolates by temperature gradient gel electrophoresis and partial sequence analysis of pcr-amplified 16s rdna variable v6 regions. | based on partial sequence analysis of pcr-amplified 16s rdna variable v6 regions of 14 enterococcal type strains, enterococcus faecalis, enterococcus mundtii, enterococcus gallinarum, enterococcus avium, enterococcus raffinosus and enterococcus saccharolyticus showed characteristic sequence motifs which made it possible to separate them into six individual species lines. furthermore, two species cluster groups could be identified, including (i) enterococcus faecium, enterococcus durans, enteroco ... | 2001 | 11430498 |
| biochemical and genetic characterization of mundticin ks, an antilisterial peptide produced by enterococcus mundtii nfri 7393. | mundticin ks, a bacteriocin produced by enterococcus mundtii nfri 7393 isolated from grass silage in thailand, is active against closely related lactic acid bacteria and the food-borne pathogen listeria monocytogenes. in this study, biochemical and genetic characterization of mundticin ks was done. mundticin ks was purified to homogeneity by ammonium sulfate precipitation, sequential ion-exchange chromatography, and solid-phase extraction. the gene cluster (mun locus) for mundticin ks production ... | 2002 | 12147478 |
| purification of bacteriocins produced by lactic acid bacteria. | bacteriocins are antibacterial substances of a proteinaceous nature that are produced by different bacterial species. lactic acid bacteria (lab) produce biologically active peptides or protein complexes that display a bactericidal mode of action almost exclusively toward gram-positive bacteria and particularly toward closely related species. generally they are active against food spoilage and foodborne pathogenic microorganisms including bacillus cereus, clostridium perfringens, staphylococcus a ... | 2004 | 15156043 |
| endophthalmitis caused by enterococcus mundtii. | enterococcus mundtii has rarely been isolated from environmental or human sources. we report the identification of e. mundtii as a pathogen of human infectious disease by dna sequencing of 16s rrna and soda genes in a case of endophthalmitis developed in a 66-year-old immunocompetent gardener. | 2005 | 15750139 |
| an antibacterial and antiviral peptide produced by enterococcus mundtii st4v isolated from soya beans. | enterococcus mundtii st4v, isolated from soya beans, produces a 3950da antibacterial peptide active against gram-positive and gram-negative bacteria, including enterococcus faecalis, streptococcus spp., pseudomonas aeruginosa, klebsiella pneumoniae, streptococcus pneumoniae and staphylococcus aureus. the peptide also inactivated the herpes simplex viruses hsv-1 (strain f) and hsv-2 (strain g), a polio virus (pv3, strain sabin) and a measles virus (strain mv/brazil/001/91, an attenuated strain of ... | 2005 | 15869868 |
| atypical genetic locus associated with the zwf gene encoding the glucose 6-phosphate dehydrogenase from enterococcus mundtii crl35. | the zwf gene encoding glucose 6-phosphate dehydrogenase (g6pd, ec.1.1.1.49) from enterococcus mundtii crl35 was cloned as a 4921 bp ecori fragment and analyzed. the predicted zwf gene product consists of 506 residues with a molecular mass of 58.4 kda, and is fully active in escherichia coli as demonstrated by its heterologous expression in the zwf-negative mutant e. coli su294. it shows a high degree of sequence identity (40-60%) to g6pds described in other bacteria. upstream of the zwf gene, a ... | 2005 | 16091851 |
| characterization of a 3944 da bacteriocin, produced by enterococcus mundtii st15, with activity against gram-positive and gram-negative bacteria. | strain st15, isolated from soy beans, and identified as enterococcus mundtii, produces a 3944 da bacteriocin that inhibits the growth of lactobacillus sakei, enterococcus faecalis, bacillus cereus, propionibacterium sp., clostridium tyrobutyricum, acinetobacter baumanii, klebsiella pneumoniae, pseudomonas aeruginosa, staphylococcus aureus, streptococcus pneumoniae and streptococcus caprinus. bacteriocin st15 is inactivated by proteinase k, pronase, pepsin, protease and triton x-114, but not when ... | 2005 | 16102864 |
| enumeration and speciation of enterococci found in marine and intertidal sediments and coastal water in southern california. | to determine the levels and species distribution of enterococci in intertidal and marine sediments and coastal waters at two beaches frequently in violation of bacterial water standards. | 2005 | 16108802 |
| fluorescent in situ hybridization with specific dna probes offers adequate detection of enterococcus faecalis and enterococcus faecium in clinical samples. | enterococcus faecalis and enterococcus faecium are among the leading causes of hospital-acquired infections. reliable and quick identification of e. faecalis and e. faecium is important for accurate treatment and understanding their role in the pathogenesis of infections. fluorescent in situ hybridization (fish) of whole bacterial cells with oligonucleotides targeted at the 16s rrna molecule leads to a reduced time to identification. in clinical practice, fish therefore can be used in situations ... | 2005 | 16157547 |
| macrolide and lincosamide resistance genes of environmental streptococci from bovine milk. | environmental streptococcus isolates from bovine milk were identified to the species and strain level and screened for resistance to macrolide and lincosamide antibiotics by phenotypic and genotypic methods. isolates were tested for resistance to erythromycin and pirlimycin by broth microdilution assays. presence of ribosomal methylase genes (erma, ermb, ermc) and efflux pump genes (mefa/e, msra/c) was detected by polymerase chain reaction (pcr). resistance to pirlimycin (minimum inhibitory conc ... | 2005 | 16207514 |
| identification and production of a bacteriocin from enterococcus mundtii qu 2 isolated from soybean. | identification of the bacteriocin produced by enterococcus mundtii qu 2 newly isolated from soybean and fermentative production of the bacteriocin. | 2005 | 16238749 |
| growth of enterococcus mundtii st15 in medium filtrate and purification of bacteriocin st15 by cation-exchange chromatography. | bacteriocin st15 (bacst15), produced by enterococcus mundtii st15, inhibited the growth of a variety of bacteria, including exopolysaccharide (eps)-producing strains isolated from biofilms in stainless steel pipes. maximal production of bacst15 (51200 au/ml) was recorded after 20 h of growth in mrs broth (biolab), which was maintained throughout fermentation. only 12800 au/ml bacst15 has been recorded in mrs filtrate with components smaller than 8000 da, suggesting that nutrients larger than 800 ... | 2005 | 16304704 |
| risk factors and outcomes associated with non-enterococcus faecalis, non-enterococcus faecium enterococcal bacteremia. | to compare risk factors, clinical features, and outcomes in patients with enterococcus avium, enterococcus casseliflavus, enterococcus durans, enterococcus gallinarum, and enterococcus mundtii bacteremia (cases) with those in patients with enterococcus faecalis bacteremia (controls). | 2006 | 16418983 |
| a taxonomic survey of lactic acid bacteria isolated from wheat (triticum durum) kernels and non-conventional flours. | in order to explore the correspondence between raw material- and mature sourdough-lactic acid bacterial (lab) communities, 59 italian wheat (triticum durum) grain samples, one bran and six non-conventional flour samples were analyzed through a culture-dependent approach. the highest cell count by an agar medium specific for lab was 2.16 log cfu/g. from about 2300 presumptive lab (gram-positive and catalase-negative) colonies collected, a total of 356 isolates were subjected to identification by ... | 2007 | 17683888 |
| a class iia peptide from enterococcus mundtii inhibits bacteria associated with otitis media. | peptide st4sa, produced by enterococcus mundtii st4sa, inhibits the growth of acinetobacter baumannii, enterococcus faecalis, enterococcus faecium, staphylococcus aureus, streptococcus pneumoniae and gram-positive bacteria isolated from patients diagnosed with middle ear infections. the peptide adsorbed at a level of 94% to s. pneumoniae 40, pseudomonas aeruginosa 25 and e. faecium hklhs. low concentrations of peptide st4sa (51200 arbitrary units (au)/ml) caused dna and enzyme leakage from targe ... | 2008 | 18155886 |
| comparative analysis of genetic diversity and incidence of virulence factors and antibiotic resistance among enterococcal populations from raw fruit and vegetable foods, water and soil, and clinical samples. | a comparative study was carried out among enterococci isolated from fruits and vegetable foods, water and soil, and clinical samples. results indicate strong differences in the numbers of enterococcal species found in different environments as well as their abundance. while enterococcus faecalis was clearly the predominant species in clinical samples, enterococcus faecium predominated in vegetables, and it slightly outnumbered e. faecalis in water samples. other species (enterococcus hirae, ente ... | 2008 | 18180067 |
| effect of gastro-intestinal conditions on the growth of enterococcus mundtii st4sa, and production of bacteriocin st4sa recorded by real-time pcr. | enterococcus mundtii st4sa, isolated from soybeans, produces a 3950 da bacteriocin (bacst4sa) active against a variety of gram-positive and gram-negative bacteria, including human pathogens. in this study, the effect of gastro-intestinal conditions on the survival of strain st4sa and production of bacst4sa was studied. strain st4sa was cultured in mrs broth at different ph and in mrs broth supplemented with bile, pancreatic enzymes, and contents of the stomach and small intestine of pigs, respec ... | 2008 | 18234384 |
| distribution and genetic relatedness of vancomycin-resistant enterococci (vre) isolated from healthy slaughtered chickens in hungary from 2001 to 2004. | the presence of the vana gene was determined in enterococci from healthy poultry, originating from the hungarian resistance monitoring system between 2001 and 2004. enterococci (n = 562) were collected from intestinal samples of slaughtered broiler chickens. the presence of van genes was detected by polymerase chain reaction (pcr). the vancomycin-resistant enterococcus (vre) strains carried only the vana gene. genus- and species-level identification of the vana gene carrier strains was carried o ... | 2008 | 18401953 |
| species distribution and antimicrobial resistance of enterococci isolated from surface and ocean water. | the species identification and antimicrobial resistance profiles were determined for enterococci isolated from southern california surface and ocean waters. | 2008 | 18422952 |
| potential of enterococci isolated from horses. | faecal samples of 122 horses (from farms in slovakia) were examined to select enterococci to study their probiotic potential for their further use as additives. each gram of faeces contained 1.0-5.0 cfu (log 10) of enterococci. of the 43 isolates, 25 (58.1%) were identified as enterococcus faecium, 3 strains were (6.9%) enterococcus mundtii and one strain was identified as e. faecalis. fourteen isolates were not characterized further. a significant proportion of the isolates were resistant to ka ... | 2008 | 18508395 |
| the role of environmental factors and medium composition on bacteriocin-like inhibitory substances (blis) production by enterococcus mundtii strains. | bacteriocin-like inhibitory substances (blis)-producers enterococcus mundtii wgwt1-1a, wgw11.2, wgj20.1, wgj40.2 and wgk53 from raw material origin were subjected to a study for the characterization of antimicrobial compound production under several growth conditions, including different cultivation media, growth temperatures, phs, different concentrations and sources of nitrogen compounds, carbohydrates and other nutritional factors, and in the presence of different percentages of ethanol and n ... | 2008 | 18541172 |
| adhesion of the probiotic strains enterococcus mundtii st4sa and lactobacillus plantarum 423 to caco-2 cells under conditions simulating the intestinal tract, and in the presence of antibiotics and anti-inflammatory medicaments. | adhesion of enterococcus mundtii st4sa and lactobacillus plantarum 423 to caco-2 (human carcinoma epithelial) cells was visualized by fluorescent staining. both strains showed good adhesion compared to l. casei mb1, l. casei shirota, l. johnsonii la1 and l. rhamnosus gg. no correlation was found between hydrophobicity, aggregation and adhesion to caco-2 cells. presence of antibiotics and anti-inflammatory medicaments reduced adhesion of bacterial strains to caco-2 cells. proteins sensitive to pe ... | 2008 | 18641972 |
| evaluation of enterococcus mundtii st4sa and lactobacillus plantarum 423 as probiotics by using a gastro-intestinal model with infant milk formulations as substrate. | enterococcus mundtii st4sa and lactobacillus plantarum 423 produce bacteriocins with activity against a number of gram-positive and gram-negative bacteria. both strains survived intestinal conditions simulated in a gastro-intestinal model (gim) with infant milk formulations as substrate and prevented the growth of listeria monocytogenes scotta. the strains are inhibited by the antibiotics amoxicillin, cefadroxil, roxithromycin and doxycycline, anti-inflammatory medicaments containing meloxicam, ... | 2008 | 18963159 |
| effect of modified mrs medium on production and purification of antimicrobial peptide st4sa produced by enterococcus mundtii. | highest antimicrobial activity of peptide st4sa (51,200 au/ml) was recorded after 14 h of growth in mrs broth with optimal production at ph 6.0 or 6.5. growth of strain st4sa in the presence of tryptone, yeast extract, or a combination of the two, yielded 102,400 au/ml. an increase in production of peptide st4sa to 102,400 au/ml was recorded in the presence of 20.0 g/l fructose, but decreased to 25,600 au/ml in the presence of lactose (20.0 g/l) or mannose (20.0 g/l) as sole carbon source. lower ... | 2009 | 19100330 |
| enterococcus faecalis with the gelatinase phenotype regulated by the fsr operon and with biofilm-forming capacity are common in the agricultural environment. | the prevalence of gelatinase activity and biofilm formation among environmental enterococci was assessed. in total, 396 enterococcal isolates from swine and cattle faeces and house flies from a cattle farm were screened for gelatinase activity. the most prevalent phenotype on todd-hewitt agar with 1.5% skim milk was the weak protease (wp) (72.2% of isolates), followed by the strong protease (sp) 18.7%, and no protease (np) (9.1%). the majority of wp isolates was represented by enterococcus hirae ... | 2009 | 19222538 |
| molecular diversity and transferability of the tetracycline resistance gene tet(m), carried on tn916-1545 family transposons, in enterococci from a total food chain. | in the present study, 20 enterococci belonging to the species enterococcus faecalis (12 strains), enterococcus faecium (4), enterococcus durans (2), enterococcus hirae (1) and enterococcus mundtii (1) and originating from a total production chain of swine meat commodities were analysed to investigate the diversity of their tetracycline resistance gene tet(m). pcr-rflp and sequence analysis showed that the tet(m) gene of most strains can be correlated with the tn916 transposon. conversely, tet(m) ... | 2009 | 19333776 |
| characterization of mundticin l, a class iia anti-listeria bacteriocin from enterococcus mundtii cugf08. | enterococcus mundtii cugf08, a lactic acid bacterium isolated from alfalfa sprouts, was found to produce mundticin l, a new class iia bacteriocin that has a high level of inhibitory activity against the genus listeria. the plasmid-associated operons containing genes for the mundticin l precursor, the atp binding cassette (abc) transporter, and immunity were cloned and sequenced. the fifth residue of the conservative consensus sequence ygngx in the mature bacteriocin is leucine instead of valine ... | 2009 | 19581470 |
| population structure and safety aspects of enterococcus strains isolated from artisanal dry fermented sausages produced in argentina. | enterococci population from argentinean artisanal dry fermented sausage was identified and their safety aspects were evaluated. species-specific pcr was used to distinguish between enterococcus faecium (56%) and enterococcus faecalis (17%). other isolates (27%) were identified as enterococcus durans, enterococcus casseliflavus and enterococcus mundtii by using 16s rna gene sequence. rapd analyses showed different biotypes for ent. faecium and ent. faecalis species. low incidence of antibiotic re ... | 2009 | 19627479 |
| characterization and identification of proteolytic bacteria from the gut of the velvetbean caterpillar (lepidoptera: noctuidae). | the characterization and identification of proteolytic bacteria from the gut of the velvetbean caterpillar (anticarsia gemmatalis) were the objectives of this study. twelve aerobic and anaerobic isolates of proteolytic bacteria were obtained from the caterpillar gut in calcium caseinate agar. the number of colony forming units (cfus) of proteolytic bacteria was higher when the bacteria were extracted from caterpillars reared on artificial diet rather than on soybean leaves (1.73 +/- 0.35 x 10(3) ... | 2009 | 19689886 |
| characterization of enterococci populations collected from a subsurface flow constructed wetland. | the aim of this study was to identify and characterize the population of enterococcus sp. in domestic wastewater as it flows through a constructed wetland. | 2010 | 19778344 |
| enterococcus mundtii st4sa and lactobacillus plantarum 423 alleviated symptoms of salmonella infection, as determined in wistar rats challenged with salmonella enterica serovar typhimurium. | wistar rats were administered daily with lactobacillus plantarum 423 and enterococcus mundtii st4sa through intragastric gavage (1 x 10(8) cfu of each strain and a combination of the two strains). sterile saline was used as placebo. after 7 days, the animals were challenged by infection with 2 x 10(8) cfu salmonella enterica serovar typhimurium. after 1 day of treatment with l. plantarum 423 and e. mundtii st4sa, the feed and water intake, and body weight of the rats increased. the faecal moistu ... | 2010 | 20127245 |
| enterococcus species composition determined by capillary electrophoresis of the groesl gene spacer region dna. | marine recreational beaches are monitored for fecal contamination by enterococcus spp. (ent) counts. although different ent species in the environment tend to thrive in and originate from distinct hosts, the current monitoring method does not differentiate among species. time-consuming isolation-based species identification precludes routine analysis of environmental ent communities. therefore, an isolation-independent dna fingerprinting method was developed to characterize environmental ent com ... | 2010 | 20639015 |
| survival and adherence of antimicrobial peptide st4sa, produced by enterococcus mundtii, at conditions found in the human gastro-intestinal tract. | the probiotic strain enterococcus mundtii st4sa, isolated from soy beans, produces a broad-spectrum antimicrobial peptide. the aim of this study was to determine if peptide st4sa could withstand conditions simulating those found in the gastro-intestinal tract (git). antimicrobial activity of peptide st4sa has been monitored by growth inhibition of enterococcus faecium on plates and leakage of β-galactosidase from damaged cells. the ability of peptide st4sa to adhere to target cells, which is the ... | 2010 | 20967791 |
| isolation and characterisation of lactic acid bacterium for effective fermentation of cellobiose into optically pure homo l-(+)-lactic acid. | effective utilisation of cellulosic biomasses for economical lactic acid production requires a microorganism with potential ability to utilise efficiently its major components, glucose and cellobiose. amongst 631 strains isolated from different environmental samples, strain qu 25 produced high yields of l-(+)-lactic acid of high optical purity from cellobiose. the qu 25 strain was identified as enterococcus mundtii based on its sugar fermentation pattern and 16s rdna sequence. the production of ... | 2011 | 21061005 |
| identification of enterococcus mundtii as a pathogenic agent involved in the "flacherie" disease in bombyx mori l. larvae reared on artificial diet. | enterococcus mundtii was shown to be directly correlated with flacherie disease of the silkworm larvae reared on artificial diet supplemented with chloramphenicol. its identification was carried out by means of light and electron microscopy and nucleotide sequencing of 16s gene. the bacterium is capable of rapidly multiplying in the silkworm gut and of invading other body tissues, as demonstrated by deliberate infection of germfree larvae and by subsequent tem observations. e. mundtii can endure ... | 2010 | 21167172 |
| efficient homofermentative l-(+)-lactic acid production from xylose by a novel lactic acid bacterium, enterococcus mundtii qu 25. | enterococcus mundtii qu 25, a newly isolated lactic acid bacterium, efficiently metabolized xylose into l-lactate. in batch fermentations, the strain produced 964 mm l-(+)-lactate from 691 mm xylose, with a yield of 1.41 mol/mol xylose consumed and an extremely high optical purity of ≥99.9% without acetate production. | 2010 | 21193678 |
| multidrug-resistant enterococci in animal meat and faeces and co-transfer of resistance from an enterococcus durans to a human enterococcus faecium. | forty-eight isolates resistant to at least two antibiotics were selected from 53 antibiotic-resistant enterococci from chicken and pig meat and faeces and analysed for specific resistance determinants. of the 48 multidrug-resistant (mdr) strains, 31 were resistant to two antibiotics (29 to erythromycin and tetracycline, 1 to erythromycin and vancomycin, 1 to vancomycin and tetracycline), 14 to three (erythromycin, tetracycline and vancomycin or ampicillin) and 3 to four (erythromycin, vancomycin ... | 2011 | 21286720 |
| diversity and dynamics of bacterial populations during spontaneous sorghum fermentations used to produce ting, a south african food. | ting is a spontaneously fermented sorghum food that is popular for its sour taste and unique flavour. insight of the microbial diversity and population dynamics during sorghum fermentations is an essential component of the development of starter cultures for commercial production of ting. in this study, bacterial populations associated with spontaneous sorghum fermentations were examined using a culture-independent strategy based on denaturing gradient gel electrophoresis and sequence analysis o ... | 2011 | 21300507 |
| potential application of the nisin z preparation of lactococcus lactis w8 in preservation of milk. | aims: the aim of the study is to evaluate the effectiveness of the preparation of nisin z from lactococcus lactis w8-fermented milk in controlling the growth of spoilage bacteria in pasteurized milk. methods and results: spoilage bacteria isolated from pasteurized milk at 8 and 15°c were identified as enterococcus italicus, enterococcus mundtii, enterococcus faecalis, bacillus thuringiensis, bacillus cereus, lactobacillus paracasei, acinetobacter sp., pseudomonas fluorescens and enterobacter a ... | 2011 | 21554339 |
| release of bacteriocins from nanofibers prepared with combinations of poly(d,l-lactide) (pdlla) and poly(ethylene oxide) (peo). | plantaricin 423, produced by lactobacillus plantarum, and bacteriocin st4sa produced by enterococcus mundtii, were electrospun into nanofibers prepared from different combinations of poly(d,l-lactide) (pdlla) and poly(ethylene oxide) (peo) dissolved in n,n-dimethylformamide (dmf). both peptides were released from the nanofibers with a high initial burst and retained 88% of their original antimicrobial activity at 37 -¦c. nanofibers have the potential to serve as carrier matrix for bacteriocins a ... | 2011 | 21731433 |
| Hydrolytic fate of deoxynivalenol-3-glucoside during digestion. | Deoxynivalenol-3-ß-D-glucoside (D3G), a plant phase II metabolite of the Fusarium mycotoxin deoxynivalenol (DON), occurs in naturally contaminated wheat, maize, oat, barley and products thereof. Although considered as a detoxification product in plants, the toxicity of this substance in mammals is currently unknown. A major concern is the possible hydrolysis of the D3G conjugate back to its toxic precursor mycotoxin DON during mammalian digestion. We used in vitro model systems to investigate th ... | 2011 | 21878373 |
| Enterocin CRL35 Inhibits Listeria monocytogenes in a Murine Model. | Abstract Listeria monocytogenes is a foodborne pathogen causative of opportunistic infections. Listeriosis is associated with severe infections in pregnant women causing abortion or neonatal listeriosis. An alternative to antibiotics are safe novel bacteriocins peptides such as enterocin CRL35 with strong antilisterial activity produced by Enterococcus mundtii CRL35. In the present paper, our goal is to study the effectiveness of this peptide and the producer strain in a murine model of pregna ... | 2011 | 22011041 |
| draft genome sequence of enterococcus mundtii crl1656. | we report the draft genome sequence of enterococcus mundtii crl1656, which was isolated from the stripping milk of a clinically healthy adult holstein dairy cow from a dairy farm of the northwestern region of tucumán (argentina). the 3.10-mb genome sequence consists of 450 large contigs and contains 2,741 predicted protein-coding genes. | 2012 | 22207752 |
| Identification of lactic acid bacteria isolated from corn stovers. | One hundred and twenty-six strains were isolated from corn stover in Henan Province, China, of which 105 isolates were considered to be lactic acid bacteria (LAB) according to Gram-positive, catalase-negative and mainly metabolic lactic acid product. Analysis of the 16S ribosomal DNA sequence of 21 representative strains was used to confirm the presence of the predominant groups and to determine the phylogenetic affiliation of isolates. The sequences from the various LAB isolates showed high deg ... | 2011 | 21951900 |
| Bacteriophage lysis of enterococcus host strains: a tool for microbial source tracking? | This paper describes the isolation of Enterococcus host strains, for potential use as simple bacteriophage (phage)-based microbial source tracking (MST) tools. Presumptive Enterococcus host strains were isolated from cattle feces, raw municipal wastewater, agricultural runoff, and waters impacted by farms or wastewater treatment works (WWTW) in southern England, United Kingdom (UK). All enterococcal host strains (n = 390) were first screened for their ability to detect phage in samples of ra ... | 2011 | 22047499 |
| 23s rrna gene-based enterococci community signatures in lake pontchartrain, louisiana, usa, following urban runoff inputs after hurricane katrina. | little is known about the impacts of fecal polluted urban runoff inputs on the structure of enterococci communities in estuarine waters. this study employed a 23s rrna gene-based polymerase chain reaction (pcr) assay with newly designed genus-specific primers, ent127f-ent907r, to determine the possible impacts of hurricane katrina floodwaters via the 17th street canal discharge on the community structure of enterococci in lake pontchartrain. a total of 94 phylotypes were identified through the r ... | 2012 | 23269456 |
| colonization of the intestinal tract of the polyphagous pest spodoptera littoralis with the gfp-tagged indigenous gut bacterium enterococcus mundtii. | the alkaline gut of lepidopterans plays a crucial role in shaping communities of bacteria. enterococcus mundtii has emerged as one of the predominant gut microorganisms in the gastrointestinal tract of the major agricultural pest, spodoptera littoralis. therefore, it was selected as a model bacterium to study its adaptation to harsh alkaline gut conditions in its host insect throughout different stages of development (larvae, pupae, adults, and eggs). to date, the mechanism of bacterial survival ... | 2016 | 27379058 |
| phylogenetic analysis of antimicrobial lactic acid bacteria from farmed seabass dicentrarchus labrax. | the use of lactic acid bacteria (lab) in the prevention or reduction of fish diseases is receiving increasing attention. in the present study, 47 lab strains were isolated from farmed seabass ( dicentrarchus labrax ) and were phenotypically and phylogenetically analysed by 16s rdna and randomly amplified polymorphic dna - polymerase chain reaction (rapd-pcr). their antimicrobial effect was tested in vitro against a wide variety of pathogenic and spoilage bacteria. most of the strains isolated we ... | 2012 | 22439634 |
| proteolytic activity of gut bacteria isolated from the velvet bean caterpillar anticarsia gemmatalis. | the development of proteinase inhibitors as potential insect control agents has been constrained by insect adaptation to these compounds. the velvet bean caterpillar (anticarsia gemmatalis) is a key soybean pest species that is well-adapted to proteinase inhibitors, particularly serine-proteinase inhibitors, which are abundant in the caterpillar host. the expression of diverse proteolytic enzymes by gut symbionts may allow the velvet bean caterpillar to circumvent proteinase inhibitors produced ... | 2013 | 23392900 |
| the gut bacteria associated with camponotus japonicus mayr with culture-dependent and dgge methods. | the bacterial composition and distribution in the different gut regions of camponotus japonicus were investigated using both culture-dependent method and culture-independent method of polymerase chain reaction and denaturing gradient gel electrophoresis (pcr-dgge). five different bacterial strains were isolated using culture-dependent method, and they all belong to the phylum firmicutes, including three genera of bacteria bacillus, paenibacillus, and enterococcus. bacillus cereus and enterococcu ... | 2012 | 22878556 |
| characterisation of the spoilage bacterial microbiota in oyster gills during storage at different temperatures. | the spoilage bacterial community in oyster gill was investigated during storage at 4, 10 and 20 °c. aerobic plate counts and ph values were determined. total bacterial dna was extracted from oyster gill and bulk cells of plate count media. the major bacterial species during fresh or different temperatures storage were determined by polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge). | 2013 | 23696433 |
| antimicrobial resistance and virulence profile of enterococci isolated from poultry and cattle sources in nigeria. | this study investigated the occurrence, antimicrobial resistance and virulence of enterococcus from poultry and cattle farms. three hundred and ninety samples: cloacal/rectal swabs (n = 260) and manure (n = 130] were processed for recovery of enterococcus species. standard bacteriological methods were used to isolate, identify and characterize enterococcus species for antimicrobial susceptibility and expression of virulence traits. detection of antibiotic resistance and virulence genes was carri ... | 2017 | 27987112 |
| significance and survival of enterococci during the house fly development. | house flies are among the most important nonbiting insect pests of medical and veterinary importance. larvae develop in decaying organic substrates and their survival strictly depends on an active microbial community. house flies have been implicated in the ecology and transmission of enterococci, including multi-antibiotic-resistant and virulent strains of enterococcus faecalis. in this study, eight american type culture collection type strains of enterococci including enterococcus avium, enter ... | 2014 | 24605454 |
| safety assessment of commensal enterococci from dogs. | enterococci form a complex, diverse, and very important group of bacteria from the technological and food safety aspect, or from the health-improving aspect as probiotics. generally, enterococci are considered to be of low pathogenic potential, which is associated mostly with clinical strains. in these strains, production of virulence factors as well as resistance to many antimicrobial drugs could complicate treatment of nosocomial infections. because there is a lack of information on incidence ... | 2017 | 28316009 |
| effect of in-feed administration and withdrawal of tylosin phosphate on antibiotic resistance in enterococci isolated from feedlot steers. | tylosin phosphate is a macrolide commonly administered to cattle in north america for the control of liver abscesses. this study investigated the effect of in-feed administration of tylosin phosphate to cattle at subtherapeutic levels and its subsequent withdrawal on macrolide resistance using enterococci as an indicator bacterium. fecal samples were collected from steers that received no antibiotics and steers administered tylosin phosphate (11 ppm) in-feed for 197 days and withdrawn 28 days be ... | 2015 | 26074889 |
| prevalence of enterococcus species and their virulence genes in fresh water prior to and after storm events. | enterococcus spp. isolates (n = 286) collected from six surface water bodies in subtropical brisbane, australia, prior to and after storm events, were identified to species level and tested for the presence of seven clinically important virulence genes (vgs). enterococcus faecalis (48%), enterococcus faecium (14%), enterococcus mundtii (13%), and enterococcus casseliflavus (13%) were frequently detected at all sites. the frequency of e. faecium occurrence increased from 6% in the dry period to 1 ... | 2014 | 24494806 |
| antibiotics and heavy metals resistance and other biological characters in enterococci isolated from surface water of monte cotugno lake (italy). | considering the limited knowledge about the biological characters in enterococci isolated from surface waters, we investigated antibiotic and heavy-metal resistance, bacteriocin production, and some important virulence traits of 165 enterococci collected in water samples from monte cotugno lake, the largest artificial basin built with earth in europe. the species distribution of isolates was as follows: enterococcus faecium (80%), enterococcus faecalis (12.7%), enterococcus casseliflavus (3%), e ... | 2013 | 23485245 |
| high inhibition of paenibacillus larvae and listeria monocytogenes by enterococcus isolated from different sources in tunisia and identification of their bacteriocin genes. | a total of 300 isolates of enterococcus, from different sources including faeces of poultry, cow and sheep, raw milk, ricotta cheese and water, in tunisia, were screened for their antibacterial activity. amongst them, 59 bacteriocin-producing strains were detected and identified by molecular methods. genes encoding for enta, entp, entb, entl50a/b, as-48 and bac31 bacteriocins were targeted by pcr. the bacteriocin-producing strains were assigned to the species enterococcus faecium, enterococcus f ... | 2014 | 24698417 |
| enterococcus species diversity in fecal samples of wild marine species as determined by real-time pcr. | analyses using culture-independent molecular techniques have improved our understanding of microbial composition. the aim of this work was to identify and quantify enterococci in fecal samples of wild marine species using real-time quantitative pcr. seven enterococcus species were examined in fecal dna of south american fur seals (arctocephalus australis), subantarctic fur seals (arctocephalus tropicalis), green turtles (chelonia mydas), magellanic penguins (spheniscus magellanicus), snowy-crown ... | 2017 | 27991828 |
| environmental waters as a source of antibiotic-resistant enterococcus species in belgrade, serbia. | despite the number of studies on antibiotic-resistant enterococci from serbian clinical settings, there are no data about environmental contamination with these bacteria. thus, this study investigated the prevalence of antibiotic-resistant enterococci in belgrade, serbia. enterococcus species collected from ten surface water sites, including a lake, two major river systems, and springs, were tested. among enterococci, we found single (21.7 %), double (17.4 %), and multiple antibiotic resistance ... | 2015 | 26314345 |
| glucose triggers atp secretion from bacteria in a growth-phase-dependent manner. | atp modulates immune cell functions, and atp derived from gut commensal bacteria promotes the differentiation of t helper 17 (th17) cells in the intestinal lamina propria. we recently reported that enterococcus gallinarum, isolated from mice and humans, secretes atp. we have since found and characterized several atp-secreting bacteria. of the tested enterococci, enterococcus mundtii secreted the greatest amount of atp (>2 μm/10(8) cells) after overnight culture. glucose, not amino acids and vita ... | 2013 | 23354720 |
| inhibition of listeria monocytogenes by enterococcus mundtii isolated from soil. | two bacterial isolates with inhibitory activity against listeria monocytogenes and enterococcus faecalis were obtained from soil. genotypic and phenotypic characterization identified them as enterococcus mundtii, a species whose ability to compete with l. monocytogenes is relatively unexplored compared to other members of the genus. the thermal stability of the inhibitory factor and its sensitivity to proteolytic enzymes indicate that it is most likely a bacteriocin. both isolates grew at compar ... | 2012 | 22986201 |
| application of bacteriocinogenic enterococcus mundtii crl35 and enterococcus faecium st88ch in the control of listeria monocytogenes in fresh minas cheese. | several strains of enterococcus spp. are capable of producing bacteriocins with antimicrobial activity against important bacterial pathogens in dairy products. in this study, the bacteriocins produced by two enterococcus strains (enterococcus mundtii crl35 and enterococcus faecium st88ch), isolated from cheeses, were characterized and tested for their capability to control growth of listeria monocytogenes 426 in experimentally contaminated fresh minas cheese during refrigerated storage. both str ... | 2012 | 22850372 |
| enterococci isolated from farm ostriches and their relation to enterocins. | the present study focuses on the detection of enterococci in ostrich faeces. forty-six bacterial colonies from 140 ostriches were identified at the species level using the maldi-tof ms identification system. according to the score value evaluation, they were allotted to the species enterococcus hirae, enterococcus faecium and enterococcus mundtii confirmed also by phenotypic testing. dominated species e. hirae (34 strains) were submitted to more detailed testing. those strains e. hirae produced ... | 2016 | 26603748 |
| dynamics of microbial community during ensiling direct-cut alfalfa with and without lab inoculant and sugar. | to gain deeper insights into the clostridial community dynamics and chemical transformations during the ensiling of alfalfa. | 2017 | 28370869 |
| symbiont-derived antimicrobials contribute to the control of the lepidopteran gut microbiota. | insects develop efficient antimicrobial strategies to flourish in a bacterial world. it has long been proposed that native gut microbiota is an important component of host defense; however, the responsible species have rarely been isolated to elucidate the mechanism of action. here we show that the dominant symbiotic bacterium enterococcus mundtii associated with the generalist herbivore spodoptera littoralis actively secretes a stable class iia bacteriocin (mundticin ks) against invading bacter ... | 2017 | 28107652 |
| draft genome sequence of enterococcus mundtii sl 16, an indigenous gut bacterium of the polyphagous pest spodoptera littoralis. | | 2016 | 27826292 |
| tyrosine decarboxylase activity of enterococcus mundtii: new insights into phenotypic and genetic aspects. | few information is available about the tyraminogenic potential of the species enterococcus mundtii. in this study, two plant-derived strains of e. mundtii were selected and investigated to better understand the phenotypic behaviour and the genetic mechanisms involved in tyramine accumulation. both the strains accumulated tyramine from the beginning of exponential phase of growth, independently on the addition of tyrosine to the medium. the strains accumulated also 2-phenylethylamine, although wi ... | 2016 | 27624853 |
| draft genome sequence of enterococcus mundtii qauem2808, isolated from dahi, a fermented milk product. | enterococcus mundtii qauem2808 has been isolated from dahi, an indigenous fermented milk product of pakistan. here, we report the draft genome sequence for this strain, which consists of 160 contigs corresponding to 2,957,514 bp and a g+c content of 38.5%. | 2016 | 27635009 |
| probiotic assessment of lactobacillus plantarum 15hn and enterococcus mundtii 50h isolated from traditional dairies microbiota. | probiotics are microorganisms, which show beneficial health effects on hosts once consumed in sufficient amounts. among probiotic bacteria, the bioactive compounds from lactic acid bacteria (lab) group can be utilized as preservative agents. lab group can be isolated and characterized from traditional dairy sources. this study aimed to isolate, identify, and biologically characterize probiotic lab strains from iranian traditional dairy products. | 2016 | 27123416 |
| first case of vana-positive enterococcus mundtii in human urinary tract infection in iran. | we cultured enterococci from urinary tract infections in iranian hospitals. seven different enterococcus species (e. raffinosus, e. durans, e. hirae, e. avium, e. mundtii, e. faecium and e. faecalis) were found. seven strains were vancomycin resistant, leading to an overall vancomycin resistance rate of 3.9%. the enterococcal infection rate was high and vancomycin-resistant enterococci incidence low. we report the first vana-positive e. mundtii urinary tract infections. | 2016 | 27081495 |
| enterococcus mundtii st4sa and lactobacillus plantarum 423 excludes listeria monocytogenes from the git, as shown by bioluminescent studies in mice. | listeria monocytogenes is an opportunistic food-borne pathogen and is life-threatening to individuals with a weakened immune system. the aim of this study was to determine if lactobacillus plantarum 423 and enterococcus mundtii st4sa could prevent colonisation of l. monocytogenes in the gastro-intestinal tract (git). mice were gavaged with l. plantarum 423, e. mundtii st4sa, and a combination of the two strains, for 6 consecutive days and orally infected with a bioluminescent strain of l. monocy ... | 2016 | 26689230 |
| host and symbiont jointly control gut microbiota during complete metamorphosis. | holometabolous insects undergo a radical anatomical re-organisation during metamorphosis. this poses a developmental challenge: the host must replace the larval gut but at the same time retain symbiotic gut microbes and avoid infection by opportunistic pathogens. by manipulating host immunity and bacterial competitive ability, we study how the host galleria mellonella and the symbiotic bacterium enterococcus mundtii interact to manage the composition of the microbiota during metamorphosis. disen ... | 2015 | 26544881 |
| identification and methods for prevention of enterococcus mundtii infection in silkworm larvae, bombyx mori, reared on artificial diet. | previously, it was reported that enterococcus mundtii (e. mundtii) was associated with flacherie disease of silkworm larvae reared on artificial diet. in this study, we report that e. mundtii was isolated from diseased silkworm larvae, and validated as a pathogenic bacterium of the animal. when silkworm larva was infected with 1.04 × 10⁶ colony-forming units of e. mundtii via oral administration of diet, half population died within six days, indicating that the bacterium is pathogenic to silkwor ... | 2015 | 26193940 |
| use of the mcherry fluorescent protein to study intestinal colonization by enterococcus mundtii st4sa and lactobacillus plantarum 423 in mice. | lactic acid bacteria (lab) are natural inhabitants of the gastrointestinal tract (git) of humans and animals, and some lab species receive considerable attention due to their health benefits. although many papers have been published on probiotic lab, only a few reports have been published on the migration and colonization of the cells in the git. this is due mostly to the lack of efficient reporter systems. in this study, we report on the application of the fluorescent mcherry protein in the in ... | 2015 | 26116681 |
| identification and antimicrobial activity detection of lactic acid bacteria isolated from corn stover silage. | a total of 59 lactic acid bacteria (lab) strains were isolated from corn stover silage. according to phenotypic and chemotaxonomic characteristics, 16s ribosomal dna (rdna) sequences and reca gene polymerase chain reaction amplification, these lab isolates were identified as five species: lactobacillus (l.) plantarum subsp. plantarum, pediococcus pentosaceus, enterococcus mundtii, weissella cibaria and leuconostoc pseudomesenteroides, respectively. those strains were also screened for antimicrob ... | 2015 | 25924957 |
| an alternative allosteric regulation mechanism of an acidophilic l-lactate dehydrogenase from enterococcus mundtii 15-1a. | a plant-derived enterococcus mundtii 15-1a, that has been previously isolated from brassica rapa l. subsp. nipposinica (l.h. bailey) hanelt var. linearifolia by our group, possesses two kinds of l-lactate dehydrogenase (l-ldh): ldh-1 and ldh-2. ldh-1 was activated under low concentration of fluctose-1,6-bisphosphate (fbp) at both ph 5.5 and 7.5. although ldh-2 was also activated under the low concentration of fbp at ph 5.5, a high concentration of fbp is necessary to activate it at ph 7.5. the p ... | 2014 | 25379380 |
| fed-batch fermentation for enhanced lactic acid production from glucose/xylose mixture without carbon catabolite repression. | there has been tremendous growth in the production of optically pure l-lactic acid from lignocellulose-derived sugars. in this study, enterococcus mundtii qu 25 was used to ferment a glucose/xylose mixture to l-lactic acid. maintenance of the xylose concentration at greater than 10 g/l achieved homo-lactic acid fermentation and reduced the formation of byproducts. furthermore, carbon catabolite repression (ccr) was avoided by maintaining the glucose concentration below 25 g/l; therefore, initial ... | 2015 | 25280397 |
| genomic comparative analysis of the environmental enterococcus mundtii against enterococcal representative species. | enterococcus mundtii is a yellow-pigmented microorganism rarely found in human infections. the draft genome sequence of e. mundtii was recently announced. its genome encodes at least 2,589 genes and 57 rnas, and 4 putative genomic islands have been detected. the objective of this study was to compare the genetic content of e. mundtii with respect to other enterococcal species and, more specifically, to identify genes coding for putative virulence traits present in enterococcal opportunistic path ... | 2014 | 24942651 |
| draft genome sequence of the nonstarter bacteriocin-producing strain enterococcus mundtii crl35. | enterococcus mundtii crl35 is a bacteriocinogenic strain isolated from an artisanal cheese of northwestern argentina. here we report its draft genome sequence, consisting of 82 contigs. in silico genomic analysis of biotechnological properties was performed to determine the potential of this microorganism to be used in a food model system. | 2014 | 24855298 |
| complete genome sequence of enterococcus mundtii qu 25, an efficient l-(+)-lactic acid-producing bacterium. | enterococcus mundtii qu 25, a non-dairy bacterial strain of ovine faecal origin, can ferment both cellobiose and xylose to produce l-lactic acid. the use of this strain is highly desirable for economical l-lactate production from renewable biomass substrates. genome sequence determination is necessary for the genetic improvement of this strain. we report the complete genome sequence of strain qu 25, primarily determined using pacific biosciences sequencing technology. the e. mundtii qu 25 genome ... | 2014 | 24568933 |
| physicochemical factors differentially affect the biomass and bacteriocin production by bovine enterococcus mundtii crl1656. | bovine enterococcus mundtii crl1656 (centro de referencia para lactobacilos culture collection) produces an anti-listeria and anti-streptococcus dysgalactiae bacteriocin identified as mundticin crl1656. the strain and its bacteriocin are candidates to be included in a beneficial product to prevent bovine mastitis as an alternative to antimicrobial agents. to optimize the production of biomass and mundticin crl1656 by e. mundtii crl1656, a complete 3 × 2(4) factorial design was applied. the effec ... | 2014 | 24359825 |
| commensal symbiosis between a lactococcus lactis strain and an enterococcus mundtii strain increases cell yield in constituted broth. | to exert their beneficial effects, probiotics need to survive in the stringent conditions of the gastrointestinal tract. symbiosis between different bacteria is a potential way of enhancing this survival. in developing new probiotic cultures, we investigated the synergic effect between enterococcus mundtii ifo 13712 and 7 strains of lactococcus lactis, many of which are widely used as starter bacteria for making dairy products and have probiotic properties. the growth yield of a mixed culture of ... | 2012 | 22981578 |
| release of enterococcus mundtii bacteriocin st4sa from self-setting brushite bone cement. | maxillofacial and craniofacial surgery is on the increase, which exposes more patients at risk of acquiring microbial infections. the use of antibiotic-loaded calcium phosphate bone cements has been shown to reduce the incidence of infection. a marked increase in antibiotic-resistant pathogens, including multidrug-resistant pathogens, has been reported. this has led to the investigation of various compounds as alternatives to conventional treatments. in this paper, we report on the incorporation ... | 2011 | 26781577 |
| otitis media: a review, with a focus on alternative treatments. | otitis media (om) is the accumulation of fluids in the middle ear, with or without symptoms of inflammation. the infection is caused by dysfunction or obstruction of the eustachian tube and is most commonly diagnosed in children under the age of two. the microbiology of om differs, with streptococcus pneumoniae, non-typeable haemophilus influenzae and moraxella catarrhalis the most commonly isolated pathogens. the emergence of penicillin-resistant strep. pneumoniae, β-lactamase-producing strains ... | 2009 | 26783131 |
| safety assessment of lactobacillus plantarum 423 and enterococcus mundtii st4sa determined in trials with wistar rats. | colonization of lactobacillus plantarum 423 and enterococcus mundtii st4sa in the gastro-intestinal tract was determined by using wistar rats as model. the strains were administered through intragastric gavage over 14 days. fish with strain-specific oligonucleotide probes indicated that lact. plantarum 423 adhered to the surfaces of the ileum and the cecum. enterococcus mundtii st4sa, on the other hand, adhered to the surfaces of the cecum and colon. results obtained by dgge have shown that stra ... | 2009 | 26783128 |