genomic variability of staphylococcus aureus and the other coagulase-positive staphylococcus species estimated by macrorestriction analysis using pulsed-field gel electrophoresis.the genomic dnas of 95 culture collection and hospital staphylococcus aureus subsp. aureus strains of various origins, as well as the genomic dnas of other coagulase-positive staphylococcus species, were cleaved with restriction endonuclease smai and subjected to pulsed-field gel electrophoresis. the levels of similarity of the smai restriction patterns of the s. aureus subsp. aureus strains varied from 30 to 100%, which is considered characteristic of this species; thus, these organisms belonge ...19968573498
widespread distribution of disinfectant resistance genes among staphylococci of bovine and caprine origin in norway.we demonstrate here a widespread distribution of genes mediating efflux-based resistance to quaternary ammonium compounds (qacs) in staphylococci from unpasteurized milk from 127 dairy cattle herds and 70 dairy goat herds. qac resistance genes were identified in 21% of the cattle herds (qaca/b, smr, qacg, and qacj) and in 10% of the goat herds (qaca/b and smr). further examination of 42 qac-resistant bovine and caprine isolates revealed the following genes: qaca/b (12 isolates) was present in fo ...200516145078
reclassification of phenotypically identified staphylococcus intermedius reclassify phenotypically identified staphylococcus intermedius strains, which might include true s. intermedius strains and novel species such as staphylococcus pseudintermedius and staphylococcus delphini, we analyzed molecular phylogenies and phenotypic characteristics of 117 s. intermedius group (sig) strains tentatively identified as being s. intermedius by the rapid id32 staph assay. from phylogenetic analyses of soda and hsp60 sequences, the sig strains were divided into three clusters ...200717596353
staphylococcus spp., streptococcus canis, and arcanobacterium phocae of healthy canadian farmed mink and mink with pododermatitis.pododermatitis is a disease of concern for mink breeders in canada and worldwide, as it causes discomfort and lowers the breeding rates on farms affected by the disease. unfortunately, the etiology and pathogenesis of pododermatitis are still unknown. in this study, we compared staphylococcus spp. and streptococcus canis isolates from healthy mink with isolates from animals with pododermatitis on 2 farms in ontario. almost all hemolytic staphylococcus spp. isolated were shown to be staphylococcu ...201525852228
outbreak of neonatal diarrhea in farmed mink kits (mustella vison) associated with enterotoxigenic staphylococcus outbreak of diarrhea on a large commercial mink farm affected 5,000 of 36,000 neonatal mink kits, with 2,000 dying within a 2-week period. affected kits were severely dehydrated, and their furcoats and paws were covered with yellow- to green-tinged mucoid feces. on necropsy, the small intestines of examined animals were markedly distended by serous to mucoid fluid. microscopically, there was prominent colonization of the intestinal villar epithelium by gram-positive bacterial cocci in the abs ...201020466860
development and evaluation of a quality-controlled ribosomal sequence database for 16s ribosomal dna-based identification of staphylococcus establish an improved ribosomal gene sequence database as part of the ribosomal differentiation of microorganisms (ridom) project and to overcome the drawbacks of phenotypic identification systems and publicly accessible sequence databases, both strands of the 5' end of the 16s ribosomal dna (rdna) of 81 type and reference strains comprising all validly described staphylococcal (sub)species were sequenced. assuming a normal distribution for pairwise distances of all unique staphylococcal sequ ...200415528685
identification of staphylococcus species by 16s-23s rdna intergenic spacer pcr investigate whether 16s-23s rdna (rdna) spacer region length polymorphisms are suitable for the identification of staphylococcus strains, the 16s-23s rdna intergenic spacer region lengths of 221 strains belonging to 31 species were studied by using a pcr-based method. each species presented a specific 16s-23s pattern made of 1-8 fragments ranging from 104-771 bp, with the exception of the species staphylococcus warnei, staphylococcus caprae and staphylococcus piscifermentans, which presented ...19989734063
staphylococcus pseudintermedius sp. nov., a coagulase-positive species from animals.four staphylococcal isolates from clinical and necropsy specimens from a cat, a dog, a horse and a parrot (psittacus erithacus timneh) were found to constitute a distinct taxon. 16s rrna gene sequence analysis revealed that its closest phylogenetic relatives are staphylococcus intermedius and staphylococcus delphini. growth characteristics, biochemical features and dna-dna hybridizations demonstrated that the strains differ from these and other known species and that they represent a single, nov ...200516014483
comparative genomics of the staphylococcus intermedius group of animal pathogens.the staphylococcus intermedius group consists of three closely related coagulase-positive bacterial species including s. intermedius, staphylococcus pseudintermedius, and staphylococcus delphini. s. pseudintermedius is a major skin pathogen of dogs, which occasionally causes severe zoonotic infections of humans. s. delphini has been isolated from an array of different animals including horses, mink, and pigeons, whereas s. intermedius has been isolated only from pigeons to date. here we provide ...201222919635
a new class of quorum quenching molecules from staphylococcus species affects communication and growth of gram-negative bacteria.the knowledge that many pathogens rely on cell-to-cell communication mechanisms known as quorum sensing, opens a new disease control strategy: quorum quenching. here we report on one of the rare examples where gram-positive bacteria, the 'staphylococcus intermedius group' of zoonotic pathogens, excrete two compounds in millimolar concentrations that suppress the quorum sensing signaling and inhibit the growth of a broad spectrum of gram-negative beta- and gamma-proteobacteria. these compounds we ...201324098134
staphylococcus delphini and methicillin-resistant s. pseudintermedius in horses, canada. 201424565044
species differentiation within the staphylococcus intermedius group using a refined maldi-tof ms database.among coagulase-positive staphylococci of animal origin, the members of the staphylococcus intermedius-group (sig: s. intermedius, staphylococcus pseudintermedius and staphylococcus delphini) are important opportunistic pathogens in different animal hosts and occasionally in humans. however, the unambiguous species diagnosis of sig is often challenging. therefore, matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) -based sig-identification with bruker mic ...201424807701
characterisation of nasal staphylococcus delphini and staphylococcus pseudintermedius isolates from healthy donkeys in tunisia.staphylococcus intermedius group (sig) bacteria can colonise the nares of some animals but are also emerging pathogens in humans and animals.201524913693
enteric colonization by staphylococcus delphini in four ferret kits with diarrhoea.four, 1-to 4-week-old ferret kits were submitted to the diagnostic center for population and animal health at michigan state university for post-mortem examination. grossly, multiple bowel loops in all ferret kits were distended by mucoid faecal material. microscopically, there was no evidence of inflammation or notable alteration to the normal mucosal morphology. gram-positive coccoid bacteria colonized variable segments of the small intestine. these bacteria were identified as staphylococcus d ...201425246181
pyogenic ventriculitis and ventricular empyema associated with staphylococcus pseudintermedius in a puppy.a 40-day-old male, blue heeler puppy with hindlimb ataxia, nystagmus, apathy, motor incoordination and hyperaesthesia of the forelimbs died 3 days after the onset of clinical signs. significant gross findings included cerebellar herniation, cerebral oedema and dilation of the third and right lateral cerebral ventricles due to the accumulation of a purulent exudate. histopathological examination revealed pyogenic ventriculitis and purulent meningoencephalitis. pure colonies of a coagulase-positiv ...201728041618
metabolism of azo dyes by human skin microbiota.reduction of methyl red (mr) and orange ii (or ii) by 26 human skin bacterial species was monitored by a rapid spectrophotometric assay. the analysis indicated that skin bacteria, representing the genera staphylococcus, corynebacterium, micrococcus, dermacoccus and kocuria, were able to reduce mr by 74-100 % in 24 h, with only three species unable to reduce completely the dye in that time. among the species tested, only corynebacterium xerosis was unable to reduce or ii to any degree by 24 h, an ...201019729456
population genetic structure of the staphylococcus intermedius group: insights into agr diversification and the emergence of methicillin-resistant strains.the population genetic structure of the animal pathogen staphylococcus intermedius is poorly understood. we carried out a multilocus sequence phylogenetic analysis of isolates from broad host and geographic origins to investigate inter- and intraspecies diversity. we found that isolates phenotypically identified as s. intermedius are differentiated into three closely related species, s. intermedius, staphylococcus pseudintermedius, and staphylococcus delphini. s. pseudintermedius, not s. interme ...200717905991
antimicrobial resistance of staphylococcus pseudintermedius.staphylococcus pseudintermedius, staphylococcus intermedius and staphylococcus delphini together comprise the s. intermedius group (sig). within the sig, s. pseudintermedius represents the major pathogenic species and is involved in a wide variety of infections, mainly in dogs, but to a lesser degree also in other animal species and humans. antimicrobial agents are commonly applied to control s. pseudintermedius infections; however, during recent years s. pseudintermedius isolates have been iden ...201222681522
mustelidae are natural hosts of staphylococcus delphini group a.according to the current taxonomy, the staphylococcus intermedius group (sig) comprises of at least three distinct species. while s. intermedius and s. pseudintermedius are associated with specific hosts (pigeons and dogs, respectively), the natural host of s. delphini remains unclear. we analysed 158 sig isolates from less studied animal species belonging to the order carnivora, including mink (n=118), fox (n=33), badger (n=6) and ferret (n=1). species identification was performed by nuc pcr in ...201222542523
evaluation of oxacillin and cefoxitin disk and mic breakpoints for prediction of methicillin resistance in human and veterinary isolates of staphylococcus intermedius group.staphylococcus pseudintermedius is a coagulase-positive species that colonizes the nares and anal mucosa of healthy dogs and cats. human infections with s. pseudintermedius range in severity from bite wounds and rhinosinusitis to endocarditis; historically, these infections were thought to be uncommon, but new laboratory methods suggest that their true incidence is underreported. oxacillin and cefoxitin disk and mic tests were evaluated for the detection of meca- or mecc-mediated methicillin res ...201626607988
camp test detected staphylococcus delphini atcc 49172 beta-haemolysin production.through a camp test, we first observed a staphylococcus delphini strain (atcc 49172) to release beta-haemolysin. production of the latter in this coagulase-positive species of the 'staphylococcus intermedius group', in fact, has been labeled to be undetermined, thus far. of course, a wider number of strains have to be investigated in order to define whether this property is constitutive (like in staphylococcus (pseud)intermedius), or strain-dependent (like in staphylococcus aureus), and which cl ...201324730145
detection of airborne bacteria in a duck production facility with two different personal air sampling devices for an exposure assessment.prevalent airborne microorganisms are not well characterized in industrial animal production buildings with respect to their quantity or quality. to investigate the work-related microbial exposure, personal bioaerosol sampling during the whole working day is recommended. therefore, bioaerosol sampling in a duck hatchery and a duck house with two personal air sampling devices, a filter-based pgp and a niosh particle size separator, was performed. subsequent, quantitative and qualitative analyses ...201525093856
evaluation of pyrrolidonyl arylamidase activity in staphylococcus delphini.clinical reference textbooks lack data for pyrrolidonyl arylamidase (pyr) activity in staphylococcus delphini this study evaluated pyr activities of 21 s. delphini strains by reference broth, rapid disc, and rapid slide methods. species and subgroup identifications were confirmed by nucleic acid-based methods and included nine group a and 12 group b strains. testing by rapid pyr methods with products from four manufacturers was performed at two testing locations, and, with the exception of one s ...201728003425
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