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phylogenetic relationships of entomopathogenic nematophilic bacteria: xenorhabdus spp. and photorhabdus sp.phylogenetic relationships of xenorhabdus spp. and photorhabdus sp. were investigated on the basis of 16s rrna gene sequences. xenorhabdus spp. and photorhabdus sp. were grouped together with proteus vulgaris and arsenophonus nasoniae. this group was distant from other members of the family enterobacteriaceae. xenorhabdus japonicus, previously proposed as a new species, was nearly located to xenorhabdus nematophilus. signature nucleotides of x. japonicus were identified that distinguish it other ...19968914266
xenorhabdus japonicus sp. nov. associated with the nematode steinernema kushidai.a new species, xenorhabdus japonicus, is proposed as the bacterial symbiont of steinernema kushidai isolated from field soil in shizuoka prefecture, japan. xenorhabdus japonicus could be distinguished phenotypically and genetically from other xenorhabdus spp. the type strain of the species, sk-1, a gram-negative, facultative anaerobe and peritrichously flagellated rod, has colonies with primary and secondary forms. the strain can be differentiated from the type strain of xenorhabdus nematophilus ...199424420949
two novel insect defensins from larvae of the cupreous chafer, anomala cuprea: purification, amino acid sequences and antibacterial activity.a humoral immune response in larvae of the coleopteran insect, anomala cuprea has been examined for exploring the molecular basis of host-pathogen interactions. the antibacterial activity against the gram-positive strain, micrococcus luteus was detected at a low level in absence of injection. the activity increased strikingly in the hemolymph of the larvae challenged with escherichia coli, showing the fluctuating profile through a time course, which consists of the static induction phase, the pr ...200111719071
phylogenetic evidence for the taxonomic heterogeneity of photorhabdus luminescens.the sequences of the 16s rrna gene of 40 strains of bacterial symbionts isolated from the nematodes heterorhabditis spp. and seven bacterial symbionts of the nematodes steinernema spp. which were isolated from different geographical areas, as well as the type strain of xenorhabdus japonicus, were determined and compared to each other and to the sequences of several reference strains of members of the enterobacteriaceae. the data confirmed the separate status of the two genera of symbionts of ent ...19979103628
larvicidal activity of the symbiotic bacterium xenorhabdus japonicus from the entomopathogenic nematode steinernema kushidai against anomala cuprea (coleoptera:scarabaeidae).the entomopathogenicity of the symbiotic bacterium xenorhabdus japonicus and the nematode steinernema kushidai was determined. phase i and ii x. japonicus were cultured on an artificial medium and inoculated into the test insect or established into axenic s. kushidai populations. when 100, 1000, or 10,000 bacterial cells of phase i or ii were directly injected into the hemocoels of 3rd instar cupreous chafer, anomala cuprea, both phases in the late log period killed 100% of the larvae by the 2nd ...19968858911
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