| inhibition of morphine dependence by a lipopolysaccharide from pantoea agglomerans. | a lipopolysaccharide from pantoea agglomerans (lpsp) was purified and examined for relief of morphine dependence by observing its inhibition of the jumping of mice on naloxone-precipitate withdrawal. administration of lpsp either intravenously or intradermally showed marked inhibition of the jumping. beta-endorphin in mouse serum and brain tissue were recognized to be in synchrony with the time course of the relief. administration of tnf-alpha gave similar effect, suggesting that lpsp induces a ... | 1992 | 1421014 |
| homeostasis as regulated by activated macrophage. ii. lps of plant origin other than wheat flour and their concomitant bacteria. | in order to seek a macrophage-activating substance, lipopolysaccharide (lps) of plant origin other than that of wheat flour was surveyed. a large amount of lps (10-100 micrograms/g) was detected in laminaria japonica (kelp), curcuma longa (turmeric), undaria pinnatifida and other substances. since concomitant bacteria possibly existing in root of farm products can be considered to contribute to lps of plant origin, a count was taken of bacterial cells both dead and alive. this count revealed tha ... | 1992 | 1525958 |
| homeostasis as regulated by activated macrophage. iii. protective effect of lpsw (lipopolysaccharide (lps) of wheat flour) on gastric ulcer in mice as compared with those of other lps from various sources. | protective effect of lipopolysaccharide (lps) from various sources on gastric ulcer has been examined in mice using parenteral as well as oral route. ulcer is induced by indomethacin, stress or alcohol. lps was prepared from 6 species of bacteria (escherichia coli, pantoea agglomerans, serratia ficaria, enterobacter cloacae, bordetella pertussis, alcaligenes faecalis) and from wheat flour. when administered intravenously, lps of pantoea agglomerans was the most effective among other lps examined ... | 1992 | 1525959 |
| ph dependence and gene structure of inaa in escherichia coli. | the weak-acid-inducible locus inaa in escherichia coli was mapped to 48.6 min by p1 cotransduction of inaa mud lac fusions and linked tn10 insertions. the inaa1::lac fusion tested negative for phenotypes characteristic of mutations in the nearby locus ubig. sequence analysis of a fragment amplified by polymerase chain reaction located the inaa1::lac fusion joint within an open reading frame 311 nucleotides downstream of nrdb, transcribed in the opposite direction, encoding a 168-amino-acid polyp ... | 1992 | 1537798 |
| pantoea punctata sp. nov., pantoea citrea sp. nov., and pantoea terrea sp. nov. isolated from fruit and soil samples. | a total of 37 bacterial strains with the general characteristics of the family enterobacteriaceae were isolated from fruit and soil samples in japan as producers of 2,5-diketo-d-gluconic acid from d-glucose. these organisms were phenotypically most closely related to the genus pantoea (f. gavini, j. mergaert, a. beji, c. mielearek, d. izard, k. kersters, and j. de ley, int. j. syst. bacteriol. 39:337-345, 1989) and were divided into three phenotypic groups. we selected nine representative strain ... | 1992 | 1581180 |
| chiral linear hydroxamates as biomimetic analogues of ferrioxamine and coprogen and their use in probing siderophore-receptor specificity in bacteria and fungi. | linear hydroxamate derivatives, possessing chiral alpha-amino acid moieties, were synthesized and their iron transport activities were studied in bacteria and fungi. no growth-promoting activity could be detected in the gram-positive hydroxamate-auxotroph aureobacterium flavescens jg9. however, gram-negative enterobacteria, such as escherichia coli, pantoea agglomerans and hafnia alvei were able to utilize iron from these analogues. uptake of 55fe-labeled analogues was inhibited by sodium azide, ... | 1991 | 1657086 |
| the specificity of bacterial siderophore receptors probed by bioassays. | the ability to utilize siderophores of bacterial and fungal origin has been studied in wild-type and mutant strains of the enterobacterial genera salmonella, escherichia, shigella, moellerella, klebsiella, enterobacter, hafnia, pantoea, ewingella, tatumella, yersinia, and in the non-enterics aeromonas, pseudomonas and aureobacterium. although only a few representative strains were tested, the results show characteristic genus-specific differences in the utilization of hydroxamate and catecholate ... | 1991 | 1663779 |
| transport and utilization of ferrioxamine-e-bound iron in erwinia herbicola (pantoea agglomerans). | we have analyzed ferrioxamine-e-mediated iron uptake and metabolization in erwinia herbicola k4 (pantoea agglomerans) by means of in vivo mössbauer spectroscopy and radioactive labeling techniques. a comparison of cell spectra with the spectrum of ferrioxamine clearly demonstrates that ferrioxamine e is not accumulated in the cell, indicating a fast metal transfer. only two major components of iron metabolism can be detected, a ferric and a ferrous species. at 30 min after uptake, 86% of the int ... | 1991 | 1931438 |
| phenotypical properties of enterobacter agglomerans (pantoea agglomerans) from human, animal and plant sources. | clinical, animal and plant isolates, representing different geographical areas, were identified as enterobacter agglomerans (pantoea agglomerans) using a quantitative bacterial dot method for dna-dna hybridization. the phenotypical properties of the 65 strains were investigated by conventional test methods. no strain decarboxylated ornithine. twenty-two strains, mainly plant isolates, showed delayed acid production from alpha-methyl-glycoside, a trait which may have ecological significance. with ... | 1991 | 2036218 |
| erwinia persicinus, a new species isolated from plants. | five strains of a gram-negative, oxidase-negative, facultatively anaerobic, fermentative, motile, rod-shaped bacterium with the general characteristics of the family enterobacteriaceae were isolated from tomatoes (three strains), a banana, and a cucumber. all of the strains produced a water-soluble pink pigment. as determined by dna hybridization (hydroxyapatite method) these five strains were 85 to 100% related in 60 and 75 degrees c reactions, and related sequences exhibited 1% or less base se ... | 1990 | 2275853 |
| an ice nucleation active gene of erwinia ananas. sequence similarity to those of pseudomonas species and regions required for ice nucleation activity. | the ice nucleation active gene, inaa, of erwinia ananas in-10 has been sequenced. this gene encodes a protein composed of 1322 amino acid residues. the inaa protein contains a 1120-residue segment consisting of 70 repeats of closely related 16 amino acid motifs (r-domain), which is flanked by n- and c-terminal sequences (n- and c-domains, respectively). its primary structure is similar to, but not identical with, those of pseudomonas inaw and inaz gene products. by truncating the inaa gene to va ... | 1989 | 2599095 |
| capsular polysaccharide biosynthesis and pathogenicity in erwinia stewartii require induction by an n-acylhomoserine lactone autoinducer. | n-acylhomoserine lactone (acyl-hsl)-mediated gene expression, also called autoinduction, is conserved among diverse gram-negative bacteria. in the paradigm vibrio fischeri system, bioluminescence is autoinducible, and the lux operon requires the transcriptional activator luxr and the acyl-hsl autoinducer for expression. the production of the acyl-hsl signal molecule is conferred by the luxi gene, and luxr encodes the transcriptional regulator. we show here that erwinia stewartii, the etiological ... | 1995 | 7665477 |
| demonstration of the indolepyruvate decarboxylase gene homologue in different auxin-producing species of the enterobacteriaceae. | different enterobacteriaceae were assayed for their ability to produce the plant hormone indole-3-acetate with the aim to study the distribution of the indole-3-pyruvate pathway, which is known to be involved in the production of indole-3-acetate in a root-associated enterobacter cloacae strain. other e. cloacae strains, and also enterobacter agglomerans strains, pantoea agglomerans, klebsiella aerogenes, and klebsiella oxytoca were found to convert tryptophan into indole-3-acetate. as it was al ... | 1994 | 7704833 |
| suppression of hyperalgesia in streptozotocin-induced diabetic mice by a lipopolysaccharide from pantoea agglomerans. | the ability of a lipopolysaccharide from pantoea agglomerans (lpsp) to relieve hyperalgesia was examined by observing its inhibition of the decrease in the threshold for nociceptive perception, as determined by the tail-pinch test, in streptozotocin-induced diabetic mice. subcutaneous injection of lpsp suppressed hyperalgesia in streptozotocin-induced diabetic mice and also exerted a therapeutic effect on hyperalgesia in these animals. the present data suggest that lpsp may be effective in relie ... | 1994 | 7920422 |
| fur regulon in gram-negative bacteria. identification and characterization of new iron-regulated escherichia coli genes by a fur titration assay. | a highly sensitive genetic screen for the detection of cloned genes coding for iron-regulated and iron-storage/binding proteins was developed. the fur titration assay (furta) enabled identification of cloned iron-regulated genes from different gram-positive and gram-negative bacteria such as: bacillus subtilis, escherichia coli, pantoea agglomerans, pseudomonas putida, salmonella typhimurium, serratia marcescens and yersinia enterocolitica. an ordered e. coli cosmid library was screened for eith ... | 1994 | 8107138 |
| binding between lipopolysaccharide and cecropin a. | cecropin a (ca), a bioactive peptide, produced significant lethality to pantoea agglomerans (pa) at low concentrations. significant mortality occurred immediately after addition of ca. separate preincubations of lipopolysaccharides (lps) from the following bacteria: pa, serratia marcescens, escherichia coli (ec), and salmonella typhimurium with ca were performed prior to the bioassay. ca was also preincubated with diphosphoryl lipid a (dpl-a) from ec and s. minnesota (sm), trilinolein, palmitic, ... | 1995 | 8569759 |
| chemotaxis of alveolar macrophages and neutrophils in response to microbial products derived from organic dust. | mechanisms of chemotaxis of alveolar macrophages (ams) and neutrophils (pmns) in response to microbial products derived from organic dust were studied using the blindwell chemotaxis chamber technique. seven different known etiological agents causing respiratory symptoms were used for experiments: cell extract and endotoxin from pantoea agglomerans (synonyms: erwinia herbicola, enterobacter agglomerans), cell extracts from thermoactinomyces vulgaris and aspergillus fumigatus, protease from bacill ... | 1995 | 8705013 |
| distribution of diaminopropane and acetylspermidine in enterobacteriaceae. | polyamines of 97 strains (60 species) belonging to 18 genera of the family enterobacteriaceae were determined by high performance liquid chromatographic analysis. in addition to putrescine and cadavarine, diaminopropane was widely distributed in enterobacteriaceae and almost ubiquitously within enterobacter, pantoea, erwinia, leminorella, proteus, leclercia, morganella, klebsiella, hafnia, rahnella, serratia, and tatumella species and sporadically within citrobacter, escherichia, moellerella, pr ... | 1996 | 8742354 |
| biochemical characteristics of enterobacter agglomerans and related strains found in buckwheat seeds. | thirty strains of bacteria were randomly isolated and identified from buckwheat seeds. the phenotypic characteristics of these strains agree well with those of the enterobacter agglomerans-erwinia herbicola complex. on the basis of the difference in indole production and gas production from d-glucose, the isolates were divided into 3 phenotypic groups, viz. i, ii and iii. twenty two strains were in phenotypic group 1, which is negative for indole production and gas production from d-glucose, and ... | 1996 | 8854178 |
| first report of a human isolate of erwinia persicinus. | erwinia persicinus was first described in 1990 after being isolated from a variety of fruits and vegetables, including bananas, cucumbers, and tomatoes. in 1994, it was shown to be the causative agent of necrosis of bean pods. we now report the first human isolate of e. persicinus. the strain was isolated from the urine of an 88-year-old woman who presented with a urinary tract infection. by the hydroxyapatite method, dna from this strain was shown to be 94.5% related at 60 degrees c and 86% rel ... | 1998 | 9431957 |
| a negative regulator mediates quorum-sensing control of exopolysaccharide production in pantoea stewartii subsp. stewartii. | classical quorum-sensing (autoinduction) regulation, as exemplified by the lux system of vibrio fischeri, requires n-acyl homoserine lactone (ahl) signals to stimulate cognate transcriptional activators for the cell density-dependent expression of specific target gene systems. for pantoea stewartii subsp. stewartii, a bacterial pathogen of sweet corn and maize, the extracellular polysaccharide (eps) stewartan is a major virulence factor, and its production is controlled by quorum sensing in a po ... | 1998 | 9636211 |
| microbial communities of printing paper machines. | the microbial content of printing paper machines, running at a temperature of 45-50 degrees c and at ph 4.5-5, was studied. bacteria were prevalent colonizers of the machine wet end and the raw materials. a total of 390 strains of aerobic bacteria were isolated and 86% of these were identified to genus and species by biochemical, chemotaxonomic and phylogenetic methods. the most common bacteria found at the machine wet end were bacillus coagulans and other bacillus species, burkholderia cepacia, ... | 1998 | 9717292 |
| the gene for indole-3-acetyl-l-aspartic acid hydrolase from enterobacter agglomerans: molecular cloning, nucleotide sequence, and expression in escherichia coli. | a 5.5-kb dna fragment containing the indole-3-acetyl-aspartic acid (iaa-asp) hydrolase gene (iaasph) was isolated from enterobacter agglomerans strain gk12 using a hybridization probe based on the n-terminal amino acid sequence of the protein. the dna sequence of a 2.4-kb region of this fragment was determined and revealed a 1311-nucleotide orf large enough to encode the 45-kda iaa-asp hydrolase. a 1.5-kb dna fragment containing iaasph was subcloned into the escherichia coli expression plasmid p ... | 1998 | 9747708 |
| a pathogenicity gene isolated from the ppath plasmid of erwinia herbicola pv. gypsophilae determines host specificity. | the host range of the gall-forming bacterium erwinia herbicola pv. gypsophilae (ehg) is restricted to the gypsophila plant whereas e. herbicola pv. betae (ehb) incites galls on beet as well as gypsophila. the pathogenicity of ehg and ehb was previously shown to be dependent on a plasmid (ppath). transposon mutagenesis was used to generate mutants on the cosmid pla150 of the ppath from ehg824-1. a cluster of nonpathogenic mutations flanked by two is1327 elements was identified on a 3.2-kb ndei dn ... | 1998 | 9675891 |
| midgut bacteria in anopheles gambiae and an. funestus (diptera: culicidae) from kenya and mali. | field studies in kenya and mali investigated the prevalence of bacteria in the midguts of malaria vectors, and the potential relationship between gram-negative bacteria species and plasmodium falciparum sporozoites. midguts were dissected from 2,430 mosquitoes: 863 anopheles funestus giles and 1,037 an. gambiae s.l. giles from kenya, and 530 an. gambiae s.l. from mali. an. funestus had a higher prevalence of gram-negative bacteria (28.5%) compared with an. gambiae collected in kenya and mali (15 ... | 1998 | 9615538 |
| production of acyl-homoserine lactone quorum-sensing signals by gram-negative plant-associated bacteria. | many gram-negative bacteria regulate expression of specialized gene sets in response to population density. this regulatory mechanism, called autoinduction or quorum-sensing, is based on the production by the bacteria of a small, diffusible signal molecule called the autoinducer. in the most well-studied systems the autoinducers are n-acylated derivatives of l-homoserine lactone (acyl-hsl). signal specificity is conferred by the length, and the nature of the substitution at c-3, of the acyl side ... | 1998 | 9805399 |
| purification and characterization of gallic acid decarboxylase from pantoea agglomerans t71 | oxygen-sensitive gallic acid decarboxylase from pantoea (formerly enterobacter) agglomerans t71 was purified from a cell extract after stabilization by reducing agents. this enzyme has a molecular mass of approximately 320 kda and consists of six identical subunits. it is highly specific for gallic acid. gallic acid decarboxylase is unique among similar decarboxylases in that it requires iron as a cofactor, as shown by plasma emission spectroscopy (which revealed an iron content of 0.8 mol per m ... | 1998 | 9835557 |
| molecular recognition of siderophores: a study with cloned ferrioxamine receptors (foxa) from erwinia herbicola and yersinia enterocolitica. | the outer membrane receptor for ferrioxamines (foxaerw) of erwinia herbicola (pantoea agglomerans) was cloned from a cosmid gene bank and partially sequenced. a comparison of the partial amino acid sequence of foxaerw with the amino acid sequence of foxayer from yersinia enterocolitica revealed a high sequence homology. a functional analysis of foxaerw and foxayer receptors cloned into a fhu-negative background (hk97) revealed that ferrioxamines are recognized at very low concentrations (< 10 pm ... | 1998 | 9542066 |
| performance evaluation of disinfectant formulations using poloxamer-hydrogel biofilm-constructs. | poloxamer f127 is a di-block co-polymer of polyoxyethylene and polyoxypropylene. aqueous solutions show thermo-reversible gelation, being liquid at temperatures < 15 degrees c and robust gels at temperatures > 15 degrees c. chilled poloxamer solutions (30% w/v) were inoculated with approximately 10(4-5) cfu ml-1 of stationary phase cultures of pseudomonas aeruginosa, ps. fluorescens, pantoea agglomerans, micrococcus luteus, staphylococcus epidermidis, bacillus subtilis or listeria innocua. drops ... | 1998 | 9871316 |
| high affinity binding of albicidin phytotoxins by the alba protein from klebsiella oxytoca. | albicidins are a family of phytotoxins and antibiotics which play an important role in the pathogenesis of sugarcane leaf scald disease. the alba gene from klebsiella oxytoca encodes a protein which inactivates albicidin by heat-reversible binding. albicidin ligand binding to a recombinant alba protein, purified by means of a glutathione s-transferase gene fusion system, is an almost instant and saturable reaction. kinetic and stoichiometric analysis of the binding reaction indicated the presenc ... | 1998 | 9493391 |
| the gene for albicidin detoxification from pantoea dispersa encodes an esterase and attenuates pathogenicity of xanthomonas albilineans to sugarcane. | albicidin phytotoxins are pathogenicity factors in a devastating disease of sugarcane known as leaf scald, caused by xanthomonas albilineans. a gene (albd) from pantoea dispersa has been cloned and sequenced and been shown to code for a peptide of 235 amino acids that detoxifies albicidin. the gene shows no significant homology at the dna or protein level to any known sequence, but the gene product contains a gxsxg motif that is conserved in serine hydrolases. the albd protein, purified to homog ... | 1997 | 9275238 |
| anti-tumor effect of lipopolysaccharide by intradermal administration as a novel drug delivery system. | we examined the antitumor effect of lipopolysaccharide extracted from pantoea agglomerans, a gram-negative bacterium, using intradermal administration on murine syngeneic tumors, meth a fibrosarcoma, mh134 hepatoma and lewis lung (ll) carcinoma. the latter two tumors are known to be relatively low in immunogenicity, highly metastatic and to have low sensitivity to biological response modifiers. although the intradermal administration of lpsp had a significantly suppressive effect on the growth o ... | 1997 | 9216680 |
| dust-borne bacteria in animal sheds, schools and children's day care centres. | a total of 316 bacterial strains, including psychrophiles, mesophiles and thermophiles, were isolated and identified from indoor dusts in schools, children's day care centres and animal sheds. several species which had not previously been reported from indoor environments were found: sphingomonas, brevibacterium, nocardiopsis, deinococcus and rhodococcus/gordona. a new psychrophilic actinomycete genus was also found in animal sheds, representing a new undescribed peptidoglycan type and an unusua ... | 1999 | 10212408 |
| antitumor mechanism of intradermal administration of lipopolysaccharide. | we earlier demonstrated that 50% of the lethal dose of lipopolysaccharide (lps) from pantoea agglomerans given by the intradermal (i.d.) route is about 300 times greater than that given by the intravenous (i.v.) route, and that 400 micrograms/kg of lps administered i.d. significantly suppressed metastasis whereas administered i.v., it did not. to learn the specific mechanism involved in this i.d. administration, the fate of lps at the skin following administration and the concurrent production o ... | 1997 | 9216652 |
| structural characterization of lipid a obtained from pantoea agglomerans lipopolysaccharide. | lipopolysaccharide isolated from pantoea agglomerans showed higher priming and triggering activities for macrophages in terms of tumor necrosis factor production than other lipopolysaccharides. to identify the difference in biological activities of lipopolysaccharide of pantoea agglomerans from other lipopolysaccharides on the basis of structure, we determined the structure of the lipid a part, which is the biological center of lipopolysaccharides, by quantitative analysis, nuclear magnetic reso ... | 1997 | 9141664 |
| mechanisms of biocontrol by pantoea dispersa of sugar cane leaf scald disease caused by xanthomonas albilineans. | albicidins, a family of phytotoxins and antibiotics produced by xanthomonas albilineans, are important in sugar cane leaf scald disease development. the albicidin detoxifying bacterium pantoea dispersa (syn. erwinia herbicola) sb1403 provides very effective biocontrol against leaf scald disease in highly susceptible sugar cane cultivars. the p. dispersa gene (albd) for enzymatic detoxification of albicidin has recently been cloned and sequenced. to test the role of albicidin detoxification in bi ... | 1997 | 9134719 |
| mechanistic analysis of high antitumor effect of intradermal administration of lipopolysaccharide from pantoea agglomerans. | lipopolysaccharide from pantoea agglomerans (lpsp) has a remarkably high antitumor activity even against poorly immunogeneic tumors when given by intradermal injection combined with cyclophosphamide (cy). we have extended this study to gain an insight into the mechanism of this antitumor effect, and especially into the induction of cell mediated immunity. in immunohistological studies, extensive necrosis and marked infiltration of the inflammatory cells at the tumor were observed after intraderm ... | 1996 | 9013473 |
| identification and characterization of a pantoea citrea gene encoding glucose dehydrogenase that is essential for causing pink disease of pineapple. | pantoea citrea, a member of the family enterobacteriaceae, causes pink disease of pineapple, whose symptom is characterized by the formation of pink to brown discolorations of the infected portions of the pineapple fruit cylinder upon canning. molecular genetic approaches were applied to elucidate the mechanism responsible for this fruit discoloration. a p. citrea mutant strain, cmc6, defective in its ability to cause pink disease and fruit discoloration, was generated by nitrosoguanidine mutage ... | 1997 | 8979341 |
| endotoxins and igg antibodies as indicators of occupational exposure to the microbial contaminants of metal-working fluids. | the aim of this study was to evaluate workers' exposure to microbes and bacterial endotoxins during the use of metal-working fluids (mwf). | 1999 | 10541909 |
| distribution of tetracycline resistance genes and transposons among phylloplane bacteria in michigan apple orchards. | the extent and nature of tetracycline resistance in bacterial populations of two apple orchards with no or a limited history of oxytetracycline usage were assessed. tetracycline-resistant (tc(r)) bacteria were mostly gram negative and represented from 0 to 47% of the total bacterial population on blossoms and leaves (versus 26 to 84% for streptomycin-resistant bacteria). a total of 87 isolates were screened for the presence of specific tc(r) determinants. tc(r) was determined to be due to the pr ... | 1999 | 10543801 |
| the phylogenetic position of serratia, buttiauxella and some other genera of the family enterobacteriaceae. | the phylogenetic relationships of the type strains of 38 species from 15 genera of the family enterobacteriaceae were investigated by comparative 16s rdna analysis. several sequences of strains from the genera citrobacter, erwinia, pantoea, proteus, rahnella and serratia, analysed in this study, have been analysed previously. however, as the sequences of this study differ slightly from the published ones, they were included in the analysis. of the 23 enterobacterial genera included in an overvie ... | 1999 | 10555323 |
| isolation of pantoea agglomerans in two cases of septic monoarthritis after plant thorn and wood sliver injuries. | arthritis after plant injury is often apparently aseptic. we report two cases due to pantoea agglomerans. in one case, the bacterium was isolated only from the pediatric blood culture media, bactec peds plus, monitored in bactec 9240, and not from the other media inoculated with the joint fluid. this procedure could help improve the diagnosis of septic arthritis. | 2000 | 10618144 |
| dissimilatory metal reduction by the facultative anaerobe pantoea agglomerans sp1. | anaerobic enrichments with acetate as the electron donor and fe(iii) as the terminal electron acceptor were obtained from sediments of salt pond, a coastal marine basin near woods hole, mass. a pure culture of a facultatively anaerobic fe(iii) reducer was isolated, and 16s rrna analysis demonstrated that this organism was most closely related to pantoea (formerly enterobacter) agglomerans, a member of the family enterobacteriaceae within the gamma subdivision of the proteobacteria. this organism ... | 2000 | 10653716 |
| significance of the c-terminal domain of erwinia uredovora ice nucleation-active protein (ina u). | ice nucleation-active (ina) proteins of bacterial origin comprise three distinct domains, i.e., n-terminal (n-), central repeat (r-), and c-terminal (c-) domains, among which the r-domain is essential, and its length may be correlated with the ice nucleation activity. in addition, the short c-terminal domain of about 50 amino acid residues is indispensable for the activity. using the ina u protein of erwinia uredovora, we carried out precise mutational analyses of its c-terminus. the ice nucleat ... | 1995 | 8720147 |
| intradermal administration of lipopolysaccharide in treatment of human cancer. | lipopolysaccharide (lps) has been recognized as a potent antitumor agent in animal tumor models; however, its use in human cancer therapy has been limited to only one trial, in which lps from salmonella was given intravenously. it was not very successful because of poor tumor response and was also toxic. we originally developed lps prepared from pantoea agglomerans (lpsp), and this was a well-purified, small-molecular-mass (5 kda) agent. we chose intradermal rather than intravenous administratio ... | 1996 | 8665574 |
| genetic and biochemical characterization of the pathway in pantoea citrea leading to pink disease of pineapple. | pink disease of pineapple, caused by pantoea citrea, is characterized by a dark coloration on fruit slices after autoclaving. this coloration is initiated by the oxidation of glucose to gluconate, which is followed by further oxidation of gluconate to as yet unknown chromogenic compounds. to elucidate the biochemical pathway leading to pink disease, we generated six coloration-defective mutants of p. citrea that were still able to oxidize glucose into gluconate. three mutants were found to be af ... | 2000 | 10735866 |
| an algorithm for automated bacterial identification using matrix-assisted laser desorption/ionization mass spectrometry. | an algorithm for bacterial identification using matrix-assisted laser desorption/ionization (maldi) mass spectrometry is being developed. this mass spectral fingerprint comparison algorithm is fully automated and statistically based, providing objective analysis of samples to be identified. based on extraction of reference fingerprint ions from test spectra, this approach should lend itself well to real-world applications where samples are likely to be impure. this algorithm is illustrated using ... | 2000 | 10740862 |
| use of continuous culture to screen for lipase-producing microorganisms and interesterification of butter fat by lipase isolates. | the continuous cultivation technique was used to investigate the screening for lipase-producing microorganisms from four commercial starters suitable for the degradation of domestic wastes. using this technique, three strains of lipase-producing bacteria were isolated and identified: pantoea agglomerans (bb96cc1, bb168cc2) and pseudomonas fluorescens (bw96cc1). in addition, butter fat induced more lipase production when present in the growth medium. interesterification of butter fat triacylglyce ... | 1996 | 8640605 |
| 16s ribosomal dna sequence analysis of a large collection of environmental and clinical unidentifiable bacterial isolates. | some bacteria are difficult to identify with phenotypic identification schemes commonly used outside reference laboratories. 16s ribosomal dna (rdna)-based identification of bacteria potentially offers a useful alternative when phenotypic characterization methods fail. however, as yet, the usefulness of 16s rdna sequence analysis in the identification of conventionally unidentifiable isolates has not been evaluated with a large collection of isolates. in this study, we evaluated the utility of 1 ... | 2000 | 11015374 |
| the tonb-dependent ferrichrome receptor fcua of yersinia enterocolitica: evidence against a strict co-evolution of receptor structure and substrate specificity. | a yersinia enterocolitica receptor mutant was isolated which is impaired in ferrichrome uptake. the receptor-encoding gene fcua was cloned in escherichia coli k-12. a fcua mutant of y. enterocolitica could be complemented by the cloned dna fragment. the fcua-encoding region was sequenced and an open reading frame encoding 758 amino acids including a signal sequence of 36 amino acids was found. fcua shared 34.6% amino acid sequence homology with fata, the anguibactin receptor of vibrio anguillaru ... | 1993 | 8384682 |
| regulation of hrp genes and type iii protein secretion in erwinia amylovora by hrpx/hrpy, a novel two-component system, and hrps. | two novel regulatory components, hrpx and hrpy, of the hrp system of erwinia amylovora were identified. the hrpxy operon is expressed in rich media, but its transcription is increased threefold by low ph, nutrient, and temperature levels--conditions that mimic the plant apoplast. hrpxy is autoregulated and directs the expression of hrpl; hrpl, in turn, activates transcription of other loci in the hrp gene cluster (z.-m. wei and s. v. beer, j. bacteriol. 177:6201-6210, 1995). the deduced amino -a ... | 2000 | 11059492 |
| structure of the nifq gene from enterobacter agglomerans 333 and its overexpression in escherichia coli. | the nifq gene, involved in early stages of iron-molybdenum cofactor (femo-co) biosynthesis, was identified downstream of the nifb and niff genes of enterobacter agglomerans. this gene was cloned and its nucleotide sequence determined. the amino acid sequence, as deduced from the nucleotide sequence, revealed an accumulation of cysteine amino acid residues at the c-terminal end of the protein. the cysteine cluster showed the following consensus sequence cys-x4-cys-x2-cys-x5-cys, which is a typica ... | 1993 | 8316214 |
| effects of a lipopolysaccharide from pantoea agglomerans on the cocaine-induced place preference. | a lipopolysaccharide from pantoea agglomerans (lpsp) was purified, and its effect on the cocaine-induced place preference was examined in rats. cocaine (4 mg/kg, i.p.) produced a significant place preference. administration of lpsp (5-1000 micrograms/kg, i.p.) alone resulted in neither preference nor aversion for either the drug- or saline-associated place. however, pretreatment with lpsp (500 and 1000 micrograms/kg, i.p.) abolished the place preference that had been induced by cocaine. furtherm ... | 1994 | 8295482 |
| antinociceptive effect of lipopolysaccharide from pantoea agglomerans on streptozotocin-induced diabetic mice. | the antinociceptive effect of lipopolysaccharide from pantoea agglomerans (lpsp) in streptozotocin-induced diabetic mice was examined. although subcutaneous (s.c.) administration of lpsp produced a dose-dependent inhibition of the tail-flick response in both non-diabetic and diabetic mice, the antinociceptive response was greater in diabetic mice than in non-diabetic mice. the antinociceptive effects of lpsp in both diabetic and non-diabetic mice were significantly antagonized by s.c. administra ... | 1994 | 8137875 |
| pantoea agglomerans strain eh318 produces two antibiotics that inhibit erwinia amylovora in vitro. | pantoea agglomerans (synonym: erwinia herbicola) strain eh318 produces through antibiosis a complex zone of inhibited growth in an overlay seeded with erwinia amylovora, the causal agent of fire blight. this zone is caused by two antibiotics, named pantocin a and b. using a genomic library of eh318, two cosmids, pcpp702 and pcpp704, were identified that conferred on escherichia coli the ability to inhibit growth of e. amylovora. the two cosmids conferred different antibiotic activities on e. col ... | 2001 | 11133457 |
| dual regulation of inaa by the multiple antibiotic resistance (mar) and superoxide (soxrs) stress response systems of escherichia coli. | the roles of the marrab (multiple antibiotic resistance) operon and soxrs (superoxide response) genes in the regulation of inaa, an unlinked weak-acid-inducible gene, were studied. inaa expression was estimated from the beta-galactosidase activity of a chromosomal inaa1::lacz transcriptional fusion. marr mutations that elevate marrab transcription and engender multiple antibiotic resistance elevated inaa expression by 10- to 20-fold over that of the wild-type. similarly, one class of inaa consti ... | 1994 | 7928997 |
| the use of 16s and 16s-23s rdna to easily detect and differentiate common gram-negative orchard epiphytes. | the identification of gram-negative pathogenic and non-pathogenic bacteria commonly isolated from an orchard phylloplane may result in a time consuming and tedious process for the plant pathologist. the paper provides a simple "one-step" protocol that uses the polymerase chain reaction (pcr) to amplify intergenic spacer regions between 16s and 23s genes and a portion of 16s gene in the prokaryotic rrna genetic loci. amplified 16s rdna, and restriction fragment length polymorphisms (rflp) followi ... | 2001 | 11166101 |
| cloning and sequencing of an ice nucleation active gene of erwinia uredovora. | an ice nucleation activity gene, named inau, of the bacterium erwinia uredovora kuin-3 has been sequenced. this gene encodes a protein of 1034 amino acid residues, and its expression product, inau protein, has an 832-amino acid residue segment consisting of 52 repeats of closely related 16-amino acid motifs (r-domain), flanked by n- and c-terminal sequences (n- and c-domains, respectively). the primary structure of the inau protein is similar to those of the inaa, inaw, and inaz gene products of ... | 1994 | 7764866 |
| [immunomodulator as medicine for morphine and cocaine dependence. especially effect of lps]. | the majority of medicines developed for narcotic and stimulant dependence were agonists or antagonists of neurotransmitters and their receptors. recently it has been shown that the nervous system, the endocrine system and the immune system are regulated by mutual interaction. the nervous system and the endocrine system are influenced by an immunomodulator, and the immune system is regulated by a neurotransmitter. in this report, medicines for morphine and cocaine dependence are first surveyed an ... | 1995 | 7699580 |
| [a case report of far advanced gastric cancer with multiple liver metastasis (h3) treated with transarterial intermittent chemotherapy and intradermal administration of low molecular lipopolysaccharide (lpsp) extracted from pantoea agglomerans]. | a case of far advanced gastric cancer with multiple liver metastasis (h3) was treated with transarterial intermittent chemotherapy (5-fu: 250 mg/week, farmorubicin: 10 mg/4 weeks, mmc: 4 mg/2 weeks) and intradermal administration of low molecular lipopolysaccharide (lpsp) extracted from pantoea agglomerans. the ct examination and endoscopy showed regression of the tumor and the patient was discharged from the hospital. lpsp was given at the concentration of 0.1 microgram initially, and the dose ... | 1995 | 7574794 |
| colonization of wheat roots by an exopolysaccharide-producing pantoea agglomerans strain and its effect on rhizosphere soil aggregation | the effect of bacterial secretion of an exopolysaccharide (eps) on rhizosphere soil physical properties was investigated by inoculating strain nas206, which was isolated from the rhizosphere of wheat (triticum durum l.) growing in a moroccan vertisol and was identified as pantoea aglomerans. phenotypic identification of this strain with the biotype-100 system was confirmed by amplified ribosomal dna restriction analysis. after inoculation of wheat seedlings with strain nas206, colonization incre ... | 1998 | 9758793 |
| characterization of pucd5000 involved in pink disease color formation by pantoea citrea. | pantoea citrea, the causal agent of pink disease of pineapple, harbors a cryptic plasmid of 5229 bp. designated pucd5000. on the basis of nucleotide and amino acid sequence analyses, pucd5000 contains both replication and mobilization loci (bom and mobcabd) that are similar to those in plasmids psw100 and psw200 in pantoea stewartii and pec3 in erwinia carotovora subsp. carotovora. the survival of p. citrea on pineapple does not depend on pucd5000. however, full pink coloration development, whic ... | 1998 | 9735319 |
| 16s rdna-based identification of bacteria from conjunctival swabs by pcr and dgge fingerprinting. | establishment of a new molecular biology technique for the identification of multiple bacteria from the ocular environment, which can be applied supplementarily to cultivation in cases of severe bacterial infections. | 2001 | 11328723 |
| all-d-cecropin b: synthesis, conformation, lipopolysaccharide binding, and antibacterial activity. | cecropin b (lcb) is a natural peptide with antibacterial and antifungal properties. the enantiomer of lcb, containing all-d amino acids (dcb), was synthesized to examine its antibacterial and binding properties. the conformation of dcb was compared to its enantiomer by circular dichroism. both the l- and d-peptides showed an identical induction of alpha-helical secondary structure. however, binding studies between lipopolysaccharide (lps) and dcb or lcb were studied with a dimethylmethylene blue ... | 2001 | 11330824 |
| identification of a new site for ferrichrome transport by comparison of the fhua proteins of escherichia coli, salmonella paratyphi b, salmonella typhimurium, and pantoea agglomerans. | the fhua genes of salmonella paratyphi b, salmonella typhimurium, and pantoea agglomerans were sequenced and compared with the known fhua sequence of escherichia coli. the highly similar fhua proteins displayed the largest difference in the predicted gating loop, which in e. coli controls the permeability of the fhua channel and serves as the principal binding site for the phages t1, t5, and phi80. all the fhua proteins contained the region in the gating loops required in e. coli for ferrichrome ... | 1998 | 9683481 |
| biofilm formation by gram-negative bacteria on central venous catheter connectors: effect of conditioning films in a laboratory model. | human blood components have been shown to enhance biofilm formation by gram-positive bacteria. we investigated the effect of human blood on biofilm formation on the inner lumen of needleless central venous catheter connectors by several gram-negative bacteria, specifically enterobacter cloacae, pseudomonas aeruginosa, and pantoea agglomerans. results suggest that a conditioning film of blood components promotes biofilm formation by these organisms in an in vitro system. | 2001 | 11376074 |
| phylogenetic position of phytopathogens within the enterobacteriaceae. | the almost complete 16s rdna sequences of twenty nine plant-associated strains, representing species of the genera erwinia, pantoea and enterobacter were determined and compared with those of other members of the enterobacteriaceae. the species of the genus erwinia may be divided into three phylogenetic groups. cluster i represents the true erwinias and comprises e. amylovora, e. mallotivora, e. persicinus, e. psidii, e. rhapontici and e. tracheiphila. we propose to unite the species of cluster ... | 1998 | 9779605 |
| [ability of representatives of pantoea agglomerans, as well as bacillus subtilis and some pseudomonas species to suppress the development of phytopathgenic bacteria and micromycetes in regulating plant growth]. | the ability of representatives of pantoea agglomerans (erwinia herbicola (lohnis) dye [21]), bacillus subtilis and some species of pseudomonas genus to inhibit the growth of phytopathogenic bacteria and micromycetes and to regulate the growth of plants has been comparatively studied. the ability to inhibit the growth of mycellium of phytopathogenic fusarium avenaceum, f. gibbosum, f. oxysporum was found out in all of 13 investigated strains of p. agglomerans, while the growth of f. culmorum is i ... | 2000 | 11421000 |
| allergic alveolitis among agricultural workers in eastern poland: a study of twenty cases. | the aim of this study was to identify the specific agents which caused extrinsic allergic alveolitis (eaa) in the selected group of 20 agricultural workers from eastern poland. the microbiological analysis of the samples of plant materials or dusts reported by the patients as causing symptoms has been carried out, followed by allergological tests (inhalation challenge, agar-gel precipitation test, inhibition of leukocyte migration, skin test) with extrinsic microbial antigens. it was found that ... | 1998 | 9852490 |
| identity and antibiotic susceptibility of enterobacterial flora of salad vegetables. | the enterobacterial flora from carrots (organic and non-organic) and salad vegetables has been identified and antibiotic susceptibilities determined. pseudomonas fluorescens and pantoea (formerly enterobacter) agglomerans were the species most commonly found; the former was usually resistant to at least six of the antibiotics under test. rahnella aquatilis (often producing beta-lactamase) was also found in carrots. there were no clear differences in flora from organic and non-organic carrots. th ... | 2001 | 11463532 |
| identification of an rcsa/rcsb recognition motif in the promoters of exopolysaccharide biosynthetic operons from erwinia amylovora and pantoea stewartii subspecies stewartii. | the regulation of capsule synthesis (rcs) regulatory network is responsible for the induction of exopolysaccharide biosynthesis in many enterobacterial species. we have previously shown that two transcriptional regulators, rcsa and rcsb, do bind as a heterodimer to the promoter of amsg, the first reading frame in the operon for amylovoran biosynthesis in the plant pathogenic bacterium erwinia amylovora. we now identified a 23-base pair fragment from position -555 to -533 upstream of the translat ... | 1999 | 9920870 |
| location and survival of leaf-associated bacteria in relation to pathogenicity and potential for growth within the leaf | the growth and survival of pathogenic and nonpathogenic pseudomonas syringae strains and of the nonpathogenic species pantoea agglomerans, stenotrophomonas maltophilia, and methylobacterium organophilum were compared in the phyllosphere of bean. in general, the plant pathogens survived better than the nonpathogens on leaves under environmental stress. the sizes of the total leaf-associated populations of the pathogenic p. syringae strains were greater than the sizes of the total leaf-associated ... | 1999 | 10103233 |
| molecular identification of microorganisms from endodontic infections. | a relatively wide range of bacteria have been isolated from root canals using standard culture techniques. however, only 50% of the bacteria in the oral cavity are cultivable (s. s. socransky et al., arch. oral biol. 8:278-280, 1963); hence, bacterial diversity in endodontic infections is underestimated. this study used a pcr-based 16s rrna gene assay, followed by cloning and sequencing of 16s rrna amplicons from a small subset of samples to assess the diversity of bacteria present in infected r ... | 2001 | 11526164 |
| new types of antimicrobial compounds produced by lactobacillus plantarum. | new types of antimicrobial compounds were identified in the culture filtrate of lactobacillus plantarum vtt e-78076. activity was detected in the low molecular mass fraction separated by gel chromatography. this fraction totally inhibited the growth of the gram-negative test organism, pantoea agglomerans (enterobacter agglomerans) vtt e-90396. characteristic compounds from this fraction were identified by gc/ms-analysis and the identification was confirmed using pure commercial reference compoun ... | 1999 | 10200070 |
| production of the biocontrol agent pantoea agglomerans strain cpa-2 using commercial products and by-products. | the aim of this paper was to find the nitrogen and carbon sources that provide maximum biomass production of strain cpa-2 of the biocontrol agent pantoea agglomerans and minimum cost of media, whilst maintaining biocontrol efficacy. to reduce the cost of media, commercial products and by-products were tested. p. agglomerans can be produced using a combination of nitrogen sources such as yeast extract (5 g l(-1)) and dry beer yeast (10 g l(-1)) with inexpensive carbohydrates such as sucrose (10 g ... | 2001 | 11549003 |
| reclassification of non-pigmented erwinia herbicola strains from trees as erwinia billingiae sp. nov. | twenty-two erwinia-like strains, isolated from trees since the late fifties and belonging to a distinct phenotypic group with resemblance to pantoea agglomerans, were further characterized by conventional biochemical tests, the biolog metabolic fingerprinting system and fatty acid analysis. their phylogenetic positions were determined by comparing the 16s rrna gene sequence of a representative strain to available sequences of erwinia, pantoea, pectobacterium and brenneria species. the strains we ... | 1999 | 10319458 |
| erwinia pyrifoliae sp. nov., a novel pathogen that affects asian pear trees (pyrus pyrifolia nakai) | a novel pathogen from asian pears (pyrus pyrifolia nakai) was analysed by sequencing the 16s rdna and the adjacent intergenic region, and the data were compared to related enterobacteriaceae. the 16s rdna of the asian pear pathogen was almost identical with the sequence of erwinia amylovora, in contrast to the 16s-23s rrna intergenic transcribed spacer region of both species. a dendrogram was deduced from determined sequences of the spacer regions including those of several related species such ... | 1999 | 10319516 |
| genetic organization of the pantoea stewartii subsp. stewartii hrp gene cluster and sequence analysis of the hrpa, hrpc, hrpn, and wtse operons. | the hrp/wts gene cluster of pantoea stewartii subsp. stewartii is required for pathogenicity on sweet corn and the ability to elicit a hypersensitive response (hr) in tobacco. site-directed transposon mutagenesis and nucleotide sequencing were used to identify hrp/wts genes within the left 20 kb of this cluster. seventeen open reading frames (orfs) comprise seven genetic complementation groups. these orfs share homology with hrp and dsp genes from erwinia amylovora, erwinia chrysanthemi, and pse ... | 2001 | 11605961 |
| characterization of plasmids that encode streptomycin-resistance in bacterial epiphytes of apple. | streptomycin resistance in strains of pseudomonas syringae pv. papulans, pantoea agglomerans and a yellow-pigmented, non-fluorescent pseudomonas sp. (py), isolated from apple orchards in new york and washington states, is predominantly associated with stra-strb genes carried on conjugal plasmids (r plasmids). none of 128 resistant erwinia amylovora strains from the eastern and western usa hybridized with a stra-strb probe, smp3. resistant py strains transfered r plasmids to ps. syringae pv. papu ... | 1999 | 10347868 |
| gdhb, a gene encoding a second quinoprotein glucose dehydrogenase in pantoea citrea, is required for pink disease of pineapple. | the pink disease of pineapple, caused by the bacterium pantoea citrea, is characterized by a dark coloration on fruit slices after canning. a glucose dehydrogenase (gdh) encoded by the gdha gene has been implicated in the colour formation activity of p. citrea. in this paper it has been shown that p. citrea contains a second, homologous gdh gene and its product, gdhb, represents the main source of gdh activity in this organism. unlike gdha, gdhb is constitutively expressed during the exponential ... | 1999 | 10376838 |
| gut colonization by an ice nucleation active bacterium, erwinia (pantoea) ananas reduces the cold hardiness of mulberry pyralid larvae. | to evaluate the suitability of using ice nucleation active (ina) bacteria for the biological control of insect pests, the supercooling point (scp) of larvae of mulberry pyralid, glyphodes duplicalis, and silkworm, bombyx mori, ingesting ina strains of erwinia (pantoea) ananas and pseudomonas syringae was determined. mean scp of the guts of silkworm larvae ingesting ina strains of e. ananas ranged from -2.5 to -2.8 degrees c, being 5 degrees c higher than that in control treatments. similarly, me ... | 1999 | 10413571 |
| exploitation of gut bacteria in the locust. | | 2000 | 10706273 |
| biological control of an insect pest by gut-colonizing enterobacter cloacae transformed with ice nucleation gene. | the ice nucleation (in) gene inaa of epiphytic erwinia (pantoea) ananas in10 was transformed into enterobacter cloacae wbmh-3-cmr originated from the faeces of silkworms. the transformant designated as ent. cloacae wbmh-3-cmr(pice6s13) exhibited in activity, unlike the parent strain. the transgenic strain was ingested by mulberry pyralid larvae, fed on detached mulberry leaves, and the supercooling capacity and cold hardiness of these larvae were examined. the mean supercooling point (scp) of th ... | 2000 | 10735247 |
| biological control of postharvest pear diseases using a bacterium, pantoea agglomerans cpa-2. | epiphytic microorganisms isolated from the fruits and leaf surfaces of apples and pears were screened for antagonistic activity against penicillium expansum on pears. from 247 microorganisms tested for antagonistic properties against p. expansum, a bacterium strain identified as pantoea agglomerans (cpa-2) was selected. this bacterium was very effective against botrytis cinerea, p. expansum and rhizopus stolonifer. complete control at the three tested concentrations (2 x 10(7), 8 x 10(7) and 1 x ... | 2001 | 11759762 |
| quorum sensing in the plant pathogen erwinia carotovora subsp. carotovora: the role of expr(ecc). | the production of the main virulence determinants of the plant pathogen erwinia carotovora subsp. carotovora, the extracellular cell wall-degrading enzymes, is partly controlled by the diffusible signal molecule n-(3-oxohexanoyl)-l-homoserine lactone (ohhl). ohhl is synthesized by the product of the expi/cari gene. linked to expi we found a gene encoding a putative transcriptional regulator of the luxr-family. this gene, expr(ecc), is transcribed convergently to the expi gene and the two open re ... | 2000 | 10755301 |
| survival of pantoea agglomerans strain cpa-2 in a spray-drying process. | spray drying could be a suitable method for preserving microorganisms, as it allows large quantities of cultures to be dried at low cost. the aims of this paper were to evaluate the effects of spray-drying conditions on survival of the biocontrol agent pantoea agglomerans cpa-2, which has shown antifungal activity against penicillium expansum and penicillium digitatum on citrus fruits. various compounds cited in the bibliography as carriers were tested in our spray drying, and some salts (mgso4, ... | 2002 | 11808793 |
| occurrence and transferability of beta-lactam resistance in enterobacteriaceae isolated in children's university hospital in bratislava. | occurrence and transferability of beta-lactam resistance in 30 multi-resistant escherichia coli, klebsiella spp., enterobacter spp., pantoea agglomerans, citrobacter freundii and serratia marcescens strains isolated from children between 0 and 3 years of age is presented. the strains were resistant to ampicillin (30), cefoxitin (22), cefotaxime (30), ceftriaxone (30), ceftazidime (30) and aztreonam (28), but susceptible to cefepime (30) and imipenem (26). twenty-eight of 30 isolates possessed a ... | 2001 | 11830947 |
| enhancement of disease resistance against penaeid acute viraemia and induction of virus-inactivating activity in haemolymph of kuruma shrimp, penaeus japonicus, by oral administration of pantoea agglomerans lipopolysaccharide (lps). | | 2000 | 11016589 |
| post-harvest biological control by pantoea agglomerans (cpa-2) on golden delicious apples. | to investigate the potential of pantoea agglomerans to control the major post-harvest diseases on golden delicious apples. | 2002 | 11849352 |
| a note: gut bacteria produce components of a locust cohesion pheromone. | faecal pellets from germ-free locusts were used as culture media to determine the ability of locust gut bacteria to synthesize phenolic components of the locust cohesion pheromone. | 2002 | 11966918 |
| effect of package and storage conditions on viability and efficacy of the freeze-dried biocontrol agent pantoea agglomerans strain cpa-2. | to reduce concentrations of protective and rehydrating media and to evaluate the effect of storage temperature, packaging and atmosphere conditions on the stability of freeze-dried pantoea agglomerans cells. efficacy against penicillium digitatum of freeze-dried cells in orange fruits was also evaluated. | 2002 | 11972691 |
| functional analysis of the erwinia herbicola tutb gene and its product. | the tutb gene, which lies just downstream of tpl, has been cloned from erwinia herbicola, and its product was analyzed. despite its high sequence similarity to tryptophan transporters, tutb was found to be a tyrosine-specific transporter. tryptophan acted as a competitive inhibitor of tyrosine transport. unlike the tryptophanase operon, the tpl and tutb genes do not constitute an operon. | 2002 | 12003958 |
| natural incidence of endophytic bacteria in pea cultivars under field conditions. | pea plants grown in the field were used to study the natural incidence of endophytic bacteria in the stem. eleven pea cultivars at the flowering stage were screened for the presence of endophytic bacteria using a printing technique with surface disinfested stem cross-sections on 5% trypticase soy agar (tsa). five cultivars showed colonization. cultivar twiggy showed the highest and most consistent colonization and was further investigated. stems of cv. twiggy at the pod stage were analyzed for e ... | 2000 | 11109492 |
| impact of fluoroquinolone administration on the emergence of fluoroquinolone-resistant gram-negative bacilli from gastrointestinal flora. | we assessed the risk factors for acquisition of fluoroquinolone-resistant, gram-negative organisms in the gastrointestinal tract of hospitalized patients. we analyzed stool samples from 204 patients and recovered fluoroquinolone-resistant, gram-negative organisms from 63. receipt of fluoroquinolone during the month preceding admission was the only risk factor identified, whereas female sex, duration of hospitalization, exposure to indwelling devices, admission from another hospital, and history ... | 2001 | 11112677 |
| plant-dependent genotypic and phenotypic diversity of antagonistic rhizobacteria isolated from different verticillium host plants. | to study the effect of plant species on the abundance and diversity of bacterial antagonists, the abundance, the phenotypic diversity, and the genotypic diversity of rhizobacteria isolated from potato, oilseed rape, and strawberry and from bulk soil which showed antagonistic activity towards the soilborne pathogen verticillium dahliae kleb. were analyzed. rhizosphere and soil samples were taken five times over two growing seasons in 1998 and 1999 from a randomized field trial. bacterial isolates ... | 2002 | 12089011 |
| effect of protective agents, rehydration media and initial cell concentration on viability of pantoea agglomerans strain cpa-2 subjected to freeze-drying. | the effect of initial cell density, protective agents and rehydration media on the viability of biocontrol agent pantoea agglomerans cpa-2 when subjected to freeze-drying was studied. several additives were tested as protective agents against freeze-drying injury. maximum viability of the bacterial cells was obtained with disaccharides (survival levels > 60%). freeze-dried samples were rehydrated with several media; the highest percentage viability was obtained with 10% non-fat skim milk (100%+) ... | 2000 | 11119153 |
| the effect of nitrogen and carbon sources on growth of the biocontrol agent pantoea agglomerans strain cpa-2. | the effect of several nitrogen and carbon sources on the growth of pantoea agglomerans (strain cpa-2) was studied for the first time. | 2002 | 12100585 |
| characterization and properties of intracellular proteins after cold acclimation of the ice-nucleating bacterium pantoea agglomerans (erwinia herbicola) ifo12686. | the ice-nucleating bacterium pantoea agglomerans (erwinia herbicola) ifo12686 (ina(+)) responds to a decrease in temperature by the induction of proteins. the pattern of protein bands from strain ifo12686 following a shift in temperature from 30 to 12 degrees c could be divided into four major groups: (1) increasing protein bands, (2) decreasing protein bands, (3) increasing--decreasing protein bands, and (4) almost constant protein bands. we identified a cryoprotective function in the increasin ... | 2000 | 11161552 |
| a cold acclimation protein with refolding activity on frozen denatured enzymes. | we found that a cold acclimation protein from an ice-nucleating bacterium, patoea ananas kuin-3, has refolding activity on frozen denatured protein. based on a sds-page analysis, we confirmed that the cold shock-treated cells of strain kuin-3 could produce some cold acclimation proteins that inhibit their syntheses by the addition of chloramphenicol during the cold acclimation. among such proteins, hsc25 had refolding activity similar to groels. hsc25 was purified to apparent homogeneity by (nh4 ... | 2000 | 11210132 |
| differences in attachment of salmonella enterica serovars and escherichia coli o157:h7 to alfalfa sprouts. | numerous salmonella enterica and escherichia coli o157:h7 outbreaks have been associated with contaminated sprouts. we examined how s. enterica serovars, e. coli serotypes, and nonpathogenic bacteria isolated from alfalfa sprouts grow on and adhere to alfalfa sprouts. growth on and adherence to sprouts were not significantly different among different serovars of s. enterica, but all s. enterica serovars grew on and adhered to alfalfa sprouts significantly better than e. coli o157:h7. e. coli o15 ... | 2002 | 12324317 |