| actinomyces georgiae sp. nov., actinomyces gerencseriae sp. nov., designation of two genospecies of actinomyces naeslundii, and inclusion of a. naeslundii serotypes ii and iii and actinomyces viscosus serotype ii in a. naeslundii genospecies 2. | dnas of type strains and representative members of actinomyces groups from the human periodontal flora and from other habitats were compared by using the s1 nuclease procedure to determine their genetic relatedness. one rather common group from the human periodontal flora, previously called "actinomyces d08," is phenotypically distinct from, and genetically unrelated to, previously described species. we propose the name of actinomyces georgiae for this organism; the type strain is strain atcc 49 ... | 1990 | 2397195 |
| recent taxonomic changes for anaerobic gram-positive and selected gram-negative organisms. | the strains formerly classified as streptococcus anginosus or "streptococcus milleri" have now been recognized as three distinct species, streptococcus anginosus, streptococcus constellatus, and streptococcus intermedius. streptococcus morbillorum has been transferred into the genus gemella. four new species within the genus peptostreptococcus were recently named. a recent addition to the genus clostridium is clostridium argentinense, which includes clostridium botulinum type g. two new species ... | 1993 | 8324113 |
| rapid characterization of periodontal bacterial isolates by using fluorogenic substrate tests. | eighty-nine species of subgingival bacteria, represented by 121 reference strains and 892 patient isolates, including gram-negative, gram-positive, aerobic, facultatively anaerobic, microaerophilic, and anaerobic species, were characterized with a panel of fluorogenic, 4-methylumbelliferyl-linked substrate tests. identifications of all patient isolates were confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) of whole-cell proteins relative to reference strains. char ... | 1996 | 8789019 |
| microbiota of health, gingivitis, and initial periodontitis. | this study compared the subgingival microbiota in periodontal health, gingivitis and initial periodontitis using predominant culture and a dna probe, checkerboard hybridization method. 56 healthy adult subjects with minimal periodontal attachment loss were clinically monitored at 3-month intervals for 12 months. more sites demonstrated small increments of attachment loss than attachment gain over the monitoring period. sites, from 17 subjects, showing > or = 1.5 mm periodontal attachment loss du ... | 1998 | 9495607 |
| dominant cross-reactive antibodies generated during the response to a variety of oral bacterial species detect phosphorylcholine. | the intraperitoneal immunization of balb/c mice with subgingival plaque from advanced periodontal pockets or with certain strains of actinobacillus actinomycetemcomitans, fusobacterium nucleatum, actinomyces israelii, streptococcus mitis, or streptococcus oralis yielded frequently indistinguishable igm monoclonal antibodies which were reactive with antigens from a variety of oral bacteria. this study aimed to characterize the specificity of such monoclonal antibodies and the diversity of oral ba ... | 1999 | 10065949 |
| molecular approaches to the differentiation of actinomyces species. | the definition of the genus actinomyces relies heavily on traditional methods of taxonomy. this study sought to develop molecular tools for the identification of strains of actinomyces israelii and actinomyces gerencseriae. oligonucleotide probes were designed and one of these successfully differentiated. a. gerencseriae from ten strains of a. israelii and three other actinomyces species by dna:dna hybridization. however, probes based on known 16s rrna sequences failed to hybridize to all the st ... | 1999 | 10551170 |
| identification of oral actinomyces species using dna probes. | oral actinomyces comprise a major segment of both the supra- and subgingival microbiota; however, little is known about the distribution of individual species in different sites or clinical conditions. the purpose of the present investigation was to develop dna probes for suggested species and genotypes of oral actinomyces. whole genomic dna probes to 12 human oral species and/or serotypes were labeled with digoxigenin and used to seek cross-reactions among the taxa using the checkerboard dna-dn ... | 1999 | 10551171 |
| systemic doxycycline administration in the treatment of periodontal infections (ii). effect on antibiotic resistance of subgingival species. | the purpose of this investigation was to determine the proportion and prevalence of doxycycline resistant species in subgingival plaque samples taken during and after doxycycline administration. 20 subjects with adult periodontitis were randomly assigned to test (n = 10) or control groups (n = 10). saliva samples as well as subgingival plaque samples taken from the distal surface of 6 posterior teeth were collected at baseline. all subjects received full mouth srp and the test group systemic dox ... | 1999 | 10599905 |
| molecular analysis of bacterial species associated with childhood caries. | although substantial epidemiologic evidence links streptococcus mutans to caries, the pathobiology of caries may involve more complex communities of bacterial species. molecular methods for bacterial identification and enumeration now make it possible to more precisely study the microbiota associated with dental caries. the purpose of this study was to compare the bacteria found in early childhood caries (ecc) to those found in caries-free children by using molecular identification methods. clon ... | 2002 | 11880430 |
| human cervicofacial actinomycoses: microbiological data for 1997 cases. | actinomycoses are sporadically occurring endogenous polymicrobial inflammatory processes, in which fermentative actinomycetes of the genera actinomyces, propionibacterium, or bifidobacterium act as the principal pathogens. difficulties in diagnosing the disease in a timely and reliable fashion have led clinicians and microbiologists to grossly underestimate its medical importance. therefore, we evaluated microbiological and selected clinical data derived from 1997 culture-positive cases of human ... | 2003 | 12905132 |
| actinomyces spp. in supragingival plaque of ethnic chinese preschool children with and without active dental caries. | very limited molecular epidemiological data are available on the role of actinomyces spp. in the pathogenesis of caries in the primary dentition. therefore, we investigated their distribution in supragingival plaque of ethnic chinese preschool children from singapore and hong kong, either with or without active caries. plaque samples were taken from intact interproximal enamel areas using dental floss. bacterial genomic dna of each sample was extracted and variable regions of 16s ribosomal dna a ... | 2003 | 12925831 |
| gingival crevice microbiota from chinese patients with gingivitis or necrotizing ulcerative gingivitis. | the objective of this study was to quantitatively compare the bacterial population structure in plaque from the gingival margin of two groups of 21 chinese patients with gingivitis or necrotizing ulcerative gingivitis (nug). subjects were recruited in four dental clinics in eastern china. samples were quantitatively assessed by immunofluorescence and fluorescent in situ hybridization for taxa known to be associated with periodontal diseases. the analyses showed that the fusiform taxa (fusobacter ... | 2004 | 14871191 |
| biofilm growth of lactobacillus species is promoted by actinomyces species and streptococcus mutans. | the ability of oral bacteria to integrate within a biofilm is pivotal to their survival. a dependence on the amount of biofilm growth by noncoaggregating lactobacillus rhamnosus and lactobacillus plantarum on coculture with actinomyces naeslundii, actinomyces gerencseriae, streptococcus mutans and veillonella parvula was investigated using an artificial-mouth culture system. biofilm formation by the lactobacilli in mono-culture was poor. in coculture with actinomyces species the amount of l. rha ... | 2004 | 15327645 |
| differences in the subgingival microbiota of swedish and usa subjects who were periodontally healthy or exhibited minimal periodontal disease. | previous studies have shown differences in the mean proportions of subgingival species in samples from periodontitis subjects in different countries, which may relate to differences in diet, genetics, disease susceptibility and manifestation. the purpose of the present investigation was to determine whether there were differences in the subgingival microbiotas of swedish and american subjects who exhibited periodontal health or minimal periodontal disease. | 2005 | 15642056 |
| phenotypic and genotypic selection of microbiota surviving under dental restorations. | the effects of sealing infected carious dentine below dental restorations on the phenotypic and genotypic diversity of the surviving microbiota was investigated. it was hypothesized that the microbiota would be subject to nutrient limitation or nutrient simplification, as it would no longer have access to dietary components or salivary secretion for growth. the available nutrients would be limited primarily to serum proteins passing from the pulp through the patent dentinal tubules to the infect ... | 2005 | 15870335 |
| antimicrobial susceptibility testing of actinomyces species with 12 antimicrobial agents. | this study was conducted to assess the susceptibility of human clinical isolates of actinomyces species to 12 antimicrobial agents. | 2005 | 15972310 |
| [actinomycosis of the buttock]. | cutaneous actinomycosis of the buttocks is a rare granulomatous bacterial infection that usually starts in the perianal area. we present an exceptional case in the form of a pseudo-tumor. | 2005 | 15988359 |
| in vitro activity of amoxicillin, clindamycin, doxycycline, metronidazole, and moxifloxacin against oral actinomyces. | actinomyces spp have been increasingly associated with endodontic infections. however, the antimicrobial susceptibility of this genus has not been studied extensively. the objective of this study was to determine the susceptibility of oral isolates of actinomyces naeslundii, actinomyces gerencseriae, actinomyces israelii, actinomyces viscosus, and actinomyces odontolyticus to amoxicillin, clindamycin, doxycycline, metronidazole, and moxifloxacin using in vitro assays. the minimum inhibitory conc ... | 2007 | 17437871 |
| [study of the relationship between oral actinomyces and childhood caries]. | actinomyces are members of the indigenous oral microbiota which have been implicated in root caries, but their role in childhood caries initiation and progression is not well-understood. the distribution of actinomyces in supragingival plaque on the primary teeth and the relationship between oral actinomyces spp. and childhood caries primarily were investigated. | 2007 | 18306628 |
| mlpa diagnostics of complex microbial communities: relative quantification of bacterial species in oral biofilms. | a multitude of molecular methods are currently used for identification and characterization of oral biofilms or for community profiling. however, multiplex pcr techniques that are able to routinely identify several species in a single assay are not available. multiplex ligation-dependent probe amplification (mlpa) identifies up to 45 unique fragments in a single tube pcr. here we report a novel use of mlpa in the relative quantification of targeted microorganisms in a community of oral microbiot ... | 2008 | 18824042 |
| emended description of actinomyces naeslundii and descriptions of actinomyces oris sp. nov. and actinomyces johnsonii sp. nov., previously identified as actinomyces naeslundii genospecies 1, 2 and wva 963. | actinomyces naeslundii is an important early colonizer in the oral biofilm and consists of three genospecies (1, 2 and wva 963) which cannot be readily differentiated using conventional phenotypic testing or on the basis of 16s rrna gene sequencing. we have investigated a representative collection of type and reference strains and clinical and oral isolates (n=115) and determined the partial gene sequences of six housekeeping genes (atpa, rpob, pgi, metg, glta and gyra). these sequences identifi ... | 2009 | 19244431 |
| actinomyces massiliensis sp. nov., isolated from a patient blood culture. | gram-positive, non-spore-forming rods (strain 4401292(t)) were isolated from a human blood sample. based on cellular morphology and the results of biochemical tests, this strain was tentatively identified as belonging to an undescribed species of the genus actinomyces. phylogenetic analysis based on 16s rrna gene sequence comparison showed that the bacterium was related closely to actinomyces gerencseriae (95.1 % 16s rrna gene sequence similarity), actinomyces israelii (95.2 %), actinomyces oric ... | 2009 | 19244437 |
| empyema necessitatis revisited. | empyema necessitatis (en) is a rare disease, with unknown incidence, which has received little attention from a dermatological point of view but it is essential to recognize it because of the possibility of causing mortality if not treated properly and in time. we report a 32-year-old woman, diagnosed with nervous anorexia, with an enlarging mass on the anterior right thoracic wall. cultures showed actinomyces gerencseriae as the main etiological agent of her empyema "necessitatis". she was succ ... | 2010 | 19822479 |
| rna-oligonucleotide quantification technique (roqt) for the enumeration of uncultivated bacterial species in subgingival biofilms. | approximately 35% of the species present in subgingival biofilms are as yet uncultivated, so their role in periodontal pathogenesis is unknown. the aim of the present study was to develop a high throughput method to quantify a wide range of cultivated and uncultivated taxa in subgingival biofilm samples associated with periodontal disease or health. oligonucleotides targeting the 16s ribosomal dna gene were designed, synthesized and labeled with digoxigenin. these probes were hybridized with the ... | 2011 | 21375703 |
| prevalence of actinomyces spp. in patients with chronic periodontitis. | this study investigated the prevalence of actinomyces spp. in shallow, deep and very deep pockets of patients with chronic periodontitis compared to healthy controls and correlated the results with clinical status. twenty patients with chronic periodontitis and 15 healthy subjects were enrolled in this study. clinical indices were recorded in a six-point measurement per tooth. from each patient samples of supra and subgingival plaque were taken separately from teeth with shallow, deep and very d ... | 2015 | 26324012 |
| actinomyces spp. gene expression in root caries lesions. | the studies of the distribution of actinomyces spp. on carious and non-carious root surfaces have not been able to confirm the association of these bacteria with root caries, although they were extensively implicated as a prime suspect in root caries. | 2016 | 27640531 |
| osseointegrated implants placed at supracrestal level may harbour higher counts of a. gerencseriae and s. constellatus - a randomized, controlled pilot study. | this study aimed at evaluating the bacterial colonization in dental implants inserted in the crestal or supracrestal position and correlated it to radiographic bone measurements. | 2015 | 26499108 |
| actinomyces gerencseriae hip prosthesis infection: a case report. | actinomyces bacteria are part of the human oropharyngeal microbiota. they have been associated with abdominal, cervicofacial and thoracic infections and a few cases of joint infections have also been described. in particular, actinomyces gerencseriae, formerly described as actinomyces israelii serovar ii, has rarely been associated with human infections, mostly involving cervicofacial lesions and periodontal diseases. here, we report one case of hip prosthesis infection due to a. gerencseriae. | 2015 | 26415492 |
| identification of the actinomycete 16s ribosomal rna gene by polymerase chain reaction in oral inflammatory lesions. | to examine the histopathological characteristics of inflammatory lesions containing actinomyces based on dna sequencing. furthermore, case reports of actinomycosis in the maxillofacial region are summarized by a review of the literature. | 2013 | 24035115 |
| the association between detectable plasmatic human immunodeficiency virus (hiv) viral load and different subgingival microorganisms in brazilian adults with hiv: a multilevel analysis. | this study investigates the association between detectable plasmatic human immunodeficiency virus (hiv) viral load (hvl) and high levels of periodontal- and non-periodontal-related microorganisms in the subgingival microbiota of individuals with hiv. | 2014 | 23952074 |
| levels of selenomonas species in generalized aggressive periodontitis. | to compare the levels of selenomonas sputigena and uncultivated/unrecognized selenomonas species in subgingival biofilms from periodontally healthy subjects and from subjects with generalized aggressive periodontitis. | 2012 | 22612405 |
| adjunctive azithromycin in the treatment of aggressive periodontitis: microbiological findings of a 12-month randomized clinical trial. | to compare the subgingival microbiological outcomes of azithromycin or placebo as adjuncts to scaling and root planing (srp) in the treatment of aggressive periodontitis (agp), and to secondarily evaluate the microbiological effect of supragingival scaling in agp patients. | 2012 | 22445846 |