| chemotactic response to mucin by serpulina hyodysenteriae and other porcine spirochetes: potential role in intestinal colonization. | chemotaxis of porcine spirochetes towards a variety of mucins was measured quantitatively by a capillary method. a chemotaxis buffer consisting of 0.01 m potassium phosphate buffer (ph 7.0) and 0.2 mm l-cysteine hydrochloride was necessary for chemotaxis of spirochetes. the optimum incubation time and incubation temperature were 1 h and 40 degrees c, respectively. the mucin concentration also affected the chemotaxis observed, and a concentration of 1% (wt/vol) was near the optimum. virulent serp ... | 1994 | 8063433 |
| genetic characterisation of intestinal spirochaetes and their association with disease. | multilocus enzyme electrophoresis was used to assess genetic relationships amongst 175 isolates of anaerobic intestinal spirochaetes, including 72 isolates from individuals living in different parts of the world, 102 from pigs and one from a dog. amongst porcine isolates belonging to the genus serpulina, a possible new species was identified. all but one of the isolates from man were clustered with the canine isolate and 59 porcine isolates in a distinct group that we have previously called "ang ... | 1994 | 8176724 |
| pathogenicity of porcine intestinal spirochetes in gnotobiotic pigs. | twelve intestinal spirochete strains of porcine origin were characterized on the basis of their phenotypic properties, by multilocus enzyme electrophoresis, and by pathogenicity testing in gnotobiotic pigs. the spirochetes used included two strains of serpulina hyodysenteriae (b204 and p18a), two strains of serpulina innocens (b256 and 4/71), one strain from the proposed new genus and species "anguillina coli" (p43/6/78), and seven non-s. hyodysenteriae strains recently isolated from united king ... | 1994 | 8188364 |
| serpulina pilosicoli sp. nov., the agent of porcine intestinal spirochetosis. | phenotypic and genetic traits of porcine intestinal spirochete strain p43/6/78t (= atcc 51139t) (t = type strain), which is pathogenic and weakly beta-hemolytic, were determined in order to confirm the taxonomic position of this organism and its relationships to previously described species of intestinal spirochetes. in bhis broth, p43/6/78t cells had a doubling time of 1 to 2 h and grew to a maximum cell density of 2 x 10(9) cells per ml at 37 to 42 degrees c. they hydrolyzed hippurate, utilize ... | 1996 | 8573497 |
| pulsed-field gel electrophoresis for sub-specific differentiation of serpulina pilosicoli (formerly 'anguillina coli'). | pulsed-field gel electrophoresis (pfge) was developed for subspecific differentiation of serpulina pilosicoli, and was applied to 52 isolates recovered from cases of intestinal spirochaetosis (is) in pigs, dogs, human beings and various avian species. the technique was highly sensitive, differentiating the isolates into 40 groupings. only six groups contained more than one isolate; in five of these groups isolates with the same banding pattern were either from pigs in the same herds (four groups ... | 1996 | 8764512 |
| phenotypic characteristics of serpulina pilosicoli the agent of intestinal spirochaetosis. | the phenotypic characteristics of three serpulina pilosicoli strains isolated from humans with diarrhoea (wesb, kar, hrm7) and two porcine s. pilosicoli strains isolated from pigs with intestinal spirochaetosis (1648, 3295), were compared with the type strain of the species p43/6/78t (t = type strain) and other intestinal spirochaetes within the genus serpulina. all s. pilosicoli strains had a characteristic ultrastructural appearance, displayed similar growth rates, hydrolysed hippurate, lacked ... | 1996 | 8810504 |
| light microscopic and ultrastructural changes in the ceca of chicks inoculated with human and canine serpulina pilosicoli. | light microscopic and ultrastructural changes were observed in chicks challenged with north american serpulina pilosicoli, a weakly beta-hemolytic intestinal spirochete (wbhis) associated with human and canine intestinal spirochetosis. chicks in control groups received trypticase soy broth or canine serpulina innocens. the birds were necropsied at weekly intervals, and the ceca were processed for bacteriologic and pathologic examinations. no wbhis were isolated from the ceca of chicks in the con ... | 1996 | 8885181 |
| experimental infection of newly weaned pigs with human and porcine strains of serpulina pilosicoli. | cultures of serpulina pilosicoli 95/1000, isolated from a pig with porcine intestinal spirochetosis (pis), and s. pilosicoli wesb, isolated from an aboriginal child with diarrhea, were used to infect 5-week-old newly weaned pigs. four of 12 pigs infected with strain 95/1000 and 2 of 12 pigs infected with strain wesb became colonized and developed watery, mucoid diarrhea within 2 to 11 days postinfection. affected pigs all had moderate subacute mucosal colitis, with gross and histological changes ... | 1996 | 8890220 |
| genetic and phenotypic characterization of intestinal spirochetes colonizing chickens and allocation of known pathogenic isolates to three distinct genetic groups. | infection with intestinal spirochetes has recently been recognized as a cause of lost production in the poultry industry. little is known about these organisms, so a collection of 56 isolates originating from chickens in commercial flocks in australia, the united states, the netherlands, and the united kingdom was examined. strength of beta-hemolysis on blood agar, indole production, api zym enzyme profiles, and cellular morphology were determined, and multilocus enzyme electrophoresis was used ... | 1997 | 9003607 |
| identification of serpulina species associated with porcine colitis by biochemical analysis and pcr. | a pcr system for the detection and identification of group iv spirochetes (serpulina pilosicoli) was designed to complement biochemical tests, e.g., the hippurate hydrolysis and beta-glucosidase tests, and to verify the accuracy of a previously proposed biochemical classification system. the pcr assay was based on amplification of a segment of the 16s rrna gene. both primers were constructed to selectively amplify the 16s rrna gene of serpulina pilosicoli. all analyzed serpulina strains exhibiti ... | 1997 | 9003616 |
| identification and characterization of serpulina pilosicoli isolates recovered from the blood of critically ill patients. | the phenotypic and genetic characteristics of spirochetes isolated from the blood of one u.s. and six french patients with severe clinical disease or impaired immunity were examined. all spirochetes were anaerobic, weakly beta-hemolytic, positive for hippurate hydrolysis, and negative for beta-glucosidase activity. cell lengths ranged from 4 to 8 microm, and each isolate had between 8 and 12 periplasmic flagella per cell. these features were consistent with the spirochetes' being serpulina pilos ... | 1997 | 9003622 |
| laboratory identification and enteropathogenicity testing of serpulina pilosicoli associated with porcine colonic spirochetosis. | pathogenic intestinal spirochetes of swine include serpulina hyodysenteriae, a strongly beta-hemolytic spirochete that causes swine dysentery, and s. pilosicoli, a weakly beta-hemolytic intestinal spirochete (wbhis) that causes porcine colonic spirochetosis. because of the existence of nonpathogenic wbhis in the normal swine colon, it is important to develop laboratory procedures for accurate identification of s. pilosicoli. the purpose of the present study was to assess hippurate hydrolysis and ... | 1997 | 9211236 |
| immunoblot reactivity of polyclonal and monoclonal antibodies with periplasmic flagellar proteins flaa1 and flab of porcine serpulina species. | the periplasmic-flagellum (pf) proteins of triton x-100-soluble and triton x-100-insoluble sodium dodecyl sulfate-treated fractions from reference and field strains of serpulina hyodysenteriae, serpulina innocens, and serpulina pilosicoli were characterized by western blotting with a rabbit polyclonal antibody (pab) specific for the 44-kda pf sheath protein of s. hyodysenteriae (z. li, f. dumas, d. dubreuil, and m. jacques, j. bacteriol. 175:8000-8007, 1993) and a murine monoclonal antibody (mab ... | 1997 | 9220154 |
| unification of the genera serpulina and brachyspira, and proposals of brachyspira hyodysenteriae comb. nov., brachyspira innocens comb. nov. and brachyspira pilosicoli comb. nov. | the phylogenetic positions of serpulina hyodysenteriae, serpulina innocens, serpulina pilosicoli and brachyspira aalborgi were studied. complete 16s ribosomal dna sequences of these three species and b. aalborgi revealed that their 16s rdna sequences were related more than 96.0%. the mol% guanine plus cytosine (g+c) of b. aalborgi dna was 27.1, and was similar to those of the 3 members of the genus serpulina. the homologous rates using 31p-labeled b. aalborgi chromosome dna in dna-dna reassociat ... | 1997 | 9251055 |
| pathogenicity of three strains of serpulina pilosicoli in pigs with a naturally acquired intestinal flora. | serpulina pilosicoli is an anaerobic spirochete which has been isolated from the colons of pigs with enteric disease. the clinical and pathologic features of experimental infections of conventional pigs (born by normal farrowing with a naturally acquired intestinal flora) with three strains of s. pilosicoli were determined in order to confirm the enteropathogenicity of this species. strains were derived from the colons of british pigs with colitis and passaged 8 to 10 times during expansion and ... | 1997 | 9284139 |
| outer membrane-associated serine protease of intestinal spirochetes. | pathogenic intestinal spirochetes cause damage to the intestinal mucosa of humans and animals by an unknown mechanism. the purpose of this study was to assess the pathogenic intestinal spirochetes serpulina hyodysenteriae, serpulina pilosicoli, and brachyspira aalborgi and the non-pathogenic commensal intestinal spirochetes serpulina innocens and treponema succinifaciens for protease activity. a partially heat stable, subtilisin-like, serine protease was identified in the outer membrane of all s ... | 1997 | 9297834 |
| sub-specific differentiation of intestinal spirochaete isolates by macrorestriction fragment profiling. | macrorestriction fragment profile analysis by pfge was used to distinguish intestinal spirochaetes, some of which were isolated from cases of swine dysentery and intestinal spirochaetosis in humans, pigs, mice, chickens and dogs. macrorestriction fragment profiles using smai and sacii restriction enzymes were produced and used in statistical analysis. this permitted the division of the isolates into two major clusters. one cluster contained isolates which were identified as serpulina pilosicoli ... | 1997 | 9308176 |
| colonic spirochetal infections in nonhuman primates that were associated with brachyspira aalborgi, serpulina pilosicoli, and unclassified flagellated bacteria. | | 1997 | 9310673 |
| recognition of two new species of intestinal spirochetes: serpulina intermedia sp. nov. and serpulina murdochii sp. nov. | on the basis of dna-dna hybridization data, nine intestinal spirochete strains were grouped into five genospecies. three of these genospecies were previously recognized serpulina species, serpulina hyodysenteriae (type strain, b78), serpulina innocens (type strain, b256), and serpulina pilosicoli (type strain, p43/6/78; previously "anguillina coli"). the other two genospecies were found to be new serpulina species, for which we propose the names serpulina intermedia sp. nov. (with type strain pw ... | 1997 | 9336899 |
| restriction fragment length polymorphism of the periplasmic flagellar flaa1 gene of serpulina species. | forty-one reference and field isolates of intestinal spirochetes representing serpulina hyodysenteriae, serpulina innocens, serpulina pilosicoli, brachyspira aalborgi, and nonclassified weakly beta-hemolytic intestinal spirochetes were compared by restriction fragment length polymorphism (rflp) of the periplasmic flagellar (pf) flaa1 gene. six genetically distinct groups (i through vi), each with a unique rflp fingerprint pattern, were identified by southern blotting analysis of ecorv chromosoma ... | 1997 | 9384289 |
| experimental infection of c3h mice with avian, porcine, or human isolates of serpulina pilosicoli. | c3h/hej (lps(d)/lps(d)) and c3h/heouj (lps(n)/lps(n)) mice were infected via gastric intubation with avian, porcine, or human isolates of weakly hemolytic spirochetes classified as serpulina pilosicoli. upon histopathological examination of cecal tissue from mice infected with avian or porcine isolates, colonization of spirochetes attached end-on to the apical surface of enterocytes was observed. there were no apparent differences in severity of cecal lesions between the lipopolysaccharide (lps) ... | 1997 | 9393839 |
| isolation of serpulina pilosicoli from rectal biopsy specimens showing evidence of intestinal spirochetosis. | histologic evidence of intestinal spirochetosis (is) was found in 22 of 41 (53.7%) rectal biopsy specimens from homosexual men attending a sexually transmitted diseases clinic. serpulina pilosicoli was cultured from 11 of the is-positive biopsy specimens (50%) and from 2 specimens (10.5%) in which spirochetes were not observed. the association between seeing spirochetes in biopsy specimens and isolating s. pilosicoli was statistically significant, clearly indicating that this spirochete is the a ... | 1998 | 9431961 |
| the prevalence of serpulina pilosicoli in humans and domestic animals in the eastern highlands of papua new guinea. | in a survey of five villages in the eastern highlands of papua new guinea, serpulina pilosicoli was isolated from rectal swabs from 113 of 496 individuals (22.8%). colonization rates ranged from 22.6-30.1% in four of the villages but was only 8.6% in the other village. in comparison colonization was demonstrated in only 5 of 54 indigenous people (9.3%) and none of 76 non-indigenous people living in an urban environment in the same region. colonization did not relate to reported occurrence of dia ... | 1997 | 9440442 |
| cecal spirochetosis associated with serpulina pilosicoli in captive juvenile ring-necked pheasants. | spirochetes similar to those described in the ceca of broilers with diarrhea and in laying hens with decreased egg production and growth were identified in the ceca of captive-raised juvenile ring-necked pheasants (phasianus colchicus). the birds were submitted for diagnostic investigation of an illness characterized by a seromucoid ocular discharge, sneezing, swollen infraorbital sinuses, and weight loss. in addition to cecal spirochetosis, the birds had mild enteric coccidiosis, trichomoniasis ... | 1997 | 9454940 |
| investigations into field cases of porcine colitis with particular reference to infection with serpulina pilosicoli. | investigations into the possible causes of colitis and typhlocolitis were carried out on 85 pig units in the united kingdom between 1992 and 1996. serpulina pilosicoli was identified most commonly, occurring as the suggested primary agent on 21 (25 per cent) of the units but forming part of mixed infections on another 23 (27 per cent) of the units, the main co-infections being yersinia pseudotuberculosis (eight units), proliferative enteropathy (six units), salmonella species (four units) or ser ... | 1998 | 9549864 |
| preparation of diagnostic polyclonal and monoclonal antibodies against outer envelope proteins of serpulina pilosicoli. | the purpose of this study was to prepare specific sera for use in the rapid detection and identification of the intestinal spirochaete serpulina pilosicoli. in western blot analysis, with pig antiserum which was raised against whole cells of s. pilosicoli and absorbed with outer envelope protein extracts from s. hyodysenteriae and s. innocens, a prominent protein with mr of c. 72 kda was consistently identified in outer envelope preparations of s. pilosicoli strains. immunogold labelling demonst ... | 1998 | 9568997 |
| evaluation of day-old specific pathogen-free chicks as an experimental model for pathogenicity testing of intestinal spirochaete species. | specific pathogen-free chicks aged 1 day were challenged per os with strains of five different species of intestinal spirochaete originally isolated from pigs or human beings. a virulent strain of serpulina hyodysenteriae (wa 15) colonized chicks, causing retarded growth rate and histological changes, including caecal atrophy, epithelial and goblet cell hyperplasia, and crypt elongation. a further strain of s. hyodysenteriae (sa3), which was apparently avirulent for pigs, and a strain of serpuli ... | 1998 | 9651813 |
| detection of lawsonia intracellularis, serpulina hyodysenteriae, weakly beta-haemolytic intestinal spirochaetes, salmonella enterica, and haemolytic escherichia coli from swine herds with and without diarrhoea among growing pigs. | a polymerase chain reaction (pcr) was optimized to detect lawsonia intracellularis in faeces from naturally infected pigs. by combining a boiling procedure to extract dna and a nested pcr procedure, a detection limit at 2 x 10(2) bacterial cells per gram of faeces was achieved. the optimized pcr was used together with conventional culture techniques to detect serpulina hyodysenteriae, weakly beta-haemolytic intestinal spirochaetes (wbhis), salmonella enterica, and haemolytic escherichia coli, in ... | 1998 | 9659692 |
| canine intestinal spirochetes consist of serpulina pilosicoli and a newly identified group provisionally designated "serpulina canis" sp. nov. | the spirochetes inhabiting the large intestines of humans and animals consist of a diverse group of related organisms. intestinal spirochetosis caused by serpulina pilosicoli is a newly recognized enteric disease of human beings and animals with potential public health significance. the purpose of this study was to determine the species identity of canine intestinal spirochetes by comparing 30 isolates obtained from dogs in australia (n = 25) and the united states (n = 5) with reference strains ... | 1998 | 9666003 |
| examination of serpulina pilosicoli for attachment and invasion determinants of enterobacteria. | the spirochaete, serpulina pilosicoli, is the agent of intestinal spirochaetosis, a diarrhoeal disease of humans and other species. by mechanisms as yet unknown, large numbers of these spirochaetes intimately attach to the colonic mucosa by one cell end. in some infected individuals, the spirochaetes may invade the lamina propria and adjacent tissues, and they may cause spirochaetaemia. to examine s. pilosicoli for pathogenic determinants homologous with enterobacteria, dna was extracted from si ... | 1998 | 9711840 |
| population genetic analysis of serpulina pilosicoli and its molecular epidemiology in villages in the eastern highlands of papua new guinea. | the population genetics of serpulina pilosicoli and its molecular epidemiology in villages in the eastern highlands province of papua new guinea were investigated. multilocus enzyme electrophoresis (mlee) was used to analyse 164 isolates from humans and animals. these were divided into 33 electrophoretic types (ets), four of which contained 65% of the isolates. the mean genetic diversity (n = number of ets) for 145 human isolates was 0.18, and the mean number of alleles at five polymorphic loci ... | 1998 | 9734020 |
| serpulina alvinipulli sp. nov., a new serpulina species that is enteropathogenic for chickens. | strain c1t is an anaerobic spirochaete that causes intestinal disease in chickens. multilocus enzyme electrophoresis analysis and 16s rrna sequence comparisons have indicated that this spirochaete is a serpulina strain. in these investigations, various phenotypic and genomic properties useful for establishing a taxonomic identity for strain c1t were studied. as determined by electron microscopy, cells of the spirochaete measured 8-11 x 0.22-0.34 mum and had a typical spirochaete ultrastructure. ... | 1998 | 9734021 |
| identification of a gene sequence encoding a putative pyruvate oxidoreductase in serpulina pilosicoli. | serpulina pilosicoli is a recently described species of intestinal spirochaete which can be identified using a species-specific monoclonal antibody bjl/ac1 reactive with a 29-kda protein located in the cell envelope. a genomic library of the type strain of s. pilosicoli p43/6/78t was created in lambda zap express and screened using bjl/ac1. single positive clones were isolated and excised into the phagemid vector pbk-cmv. phagemid dna was purified and a single clone was selected for sequencing. ... | 1998 | 9741089 |
| serpulina pilosicoli, waterbirds and water: potential sources of infection for humans and other animals. | serpulina pilosicoli was isolated from 8 of 43 (19%) faecal specimens obtained from feral waterbirds sampled around a small lake at perth zoological gardens, western australia, and from 3 of 7 (43%) samples of the lake water. the organism was only isolated from 1 of 204 (0.5%) samples from captive birds and animals in the zoological collection. multilocus enzyme electrophoresis analysis of the isolates showed that they were genetically diverse, and none had identical electrophoretic profiles as ... | 1998 | 9747776 |
| specific detection of the genus serpulina, s. hyodysenteriae and s. pilosicoliin porcine intestines by fluorescent rrna in situ hybridization. | a fluorescent-labelledin situ hybridization method targeting rrna was devised to facilitate specific identification and diagnosis of diarrhoea and colitis in pigs caused by the genus serpulina, as well as to distinguish the species serpulina hyodysenteriae and serpulina pilosicoli in formalin-fixed colon tissue sections. a genus-specific oligonucleotide probe ser1410 targeting the five species of porcine serpulina was thus designed. furthermore, species specific oligonucleotide probes (hyo1210, ... | 1998 | 9778458 |
| in vitro activity of four antimicrobial agents against north american isolates of porcine serpulina pilosicoli. | porcine colonic spirochetosis is a nonfatal diarrheal disease that affects pigs during the growing and finishing stages of production. the disease is caused by serpulina pilosicoli, a newly recognized species of pathogenic intestinal spirochete. antimicrobial therapy aimed at reducing the infection may be helpful in controlling spirochetal diarrhea. in this study, the in vitro antimicrobial susceptibilities of the reference isolate s. pilosicoli p43/6/78 from the united kingdom and 19 field isol ... | 1998 | 9786523 |
| variation amongst human isolates of brachyspira (serpulina) pilosicoli based on biochemical characterization and 16s rrna gene sequencing. | brachyspira pilosicoli (formerly serpulina pilosicoli) causes swine spirochaetosis and can also be isolated fro human faeces, although its role in human disease remains unclear. the genetic and biochemical variations amongst 19 isolates of human spirochaetes from five different countries were evaluated and compared to those found amongst swine isolates of b. pilosicoli. all isolates were negative for beta-glucosidase and all but one were positive for hippurate hydrolysis, which are characteristi ... | 1998 | 9828427 |
| search for bacteriophages spontaneously occurring in cultures of haemolytic intestinal spirochaetes of human and animal origin. | an electron microscopic survey of the occurrence of bacteriophages which appear spontaneously in cultures of haemolytic intestinal spirochaetes of human and animal origin was made. excluding one isometric tailed phage particle which was observed in the form of free particle in proximity to a spirochaete of the w beta his strain hrm18, bacteriophages were never observed while examining cells of 21 weakly beta-haemolytic human intestinal spirochaetes (w beta his), swine serpulina pilosicoli strain ... | 1998 | 9871329 |
| bacteriophages induced from weakly beta-haemolytic human intestinal spirochaetes by mitomycin c. | a comparative electron microscopic analysis of weakly beta-haemolytic spirochaetes related to human and animal intestinal spirochaetosis was done in order to search for the presence of inducible bacteriophages associated with these spirochaetes. bacteriophages were detected at the electron microscope after experimental induction with mitomycin c in 4 strains of weakly beta-haemolytic spirochaetes related to human intestinal spirochaetosis, in serpulina pilosicoli strain p43/6/78, the causative a ... | 1998 | 9871330 |
| pcr amplification from fixed tissue indicates frequent involvement of brachyspira aalborgi in human intestinal spirochetosis. | pcr procedures amplifying portions of the 16s rrna and nadh oxidase genes of brachyspira aalborgi and serpulina pilosicoli were applied to dna extracted from paraffin-embedded human colonic or rectal tissues from 30 norwegian, australian, and u.s. patients, 16 of whom had histologic evidence of intestinal spirochetosis (is). b. aalborgi-specific sequences were identified by pcr in 10 of the is patients (62.5%) but none of the others, while s. pilosicoli sequences were not detected in tissues fro ... | 1999 | 10325391 |
| lipo-oligosaccharide profiles of serpulina pilosicoli strains and their serological cross-reactivities. | the purpose of this study was to determine the presence of lipopolysaccharide-like material in the intestinal spirochaete serpulina pilosicoli and the extent of antigenic cross-reactivity of this material in different strains of the species. hot water-phenol, aqueous-phase extracts from five porcine and three human strains of s. pilosicoli, and from seven strains of four other serpulina spp., were separated by sds-page and silver-stained profiles were obtained. all s. pilosicoli strains had a pr ... | 1999 | 10509486 |
| scanning electron microscopy and fluorescent in situ hybridization of experimental brachyspira (serpulina) pilosicoli infection in growing pigs. | two groups of six 8-week-old pigs were challenged with 1x10(9) cfu brachyspira (serpulina) pilosicoli or serpulina intermedia daily for 3 consecutive days to study the pathology of porcine colonic spirochetosis by scanning electron microscopy (sem) and fluorescent in situ hybridization (fish) with oligonucleotide probes targeting ribosomal rna specific for b. pilosicoli and the genus brachyspira/serpulina. six pigs served as noninoculated controls. the animals were euthanatized successively betw ... | 2000 | 10643977 |
| comparative pathology of bacterial enteric diseases of swine. | enteric bacterial infections are among the most common and economically significant diseases affecting swine production worldwide. clinical signs of these infections include diarrhea, reduced growth rate, weight loss, and death of preweaned, weanling, grower-finisher, young and adult age breeding animals. the most common etiological agents include escherichia coli, clostridium perfringens, lawsonia intracellularis, salmonella enterica, and brachyspira (serpulina) spp. with the exception of brach ... | 1999 | 10659346 |
| recovery from colonic infection elicits serum igg antibodies to specific serpulina pilosicoli outer membrane antigens (spoma). | colonic spirochetosis caused by s. pilosicoli is a disease of human and animals characterized by intimate attachment of the spirochete to colonic epithelial cells and colitis. to identify antigens that are potentially involved in recovery from the disease, whole-cell lysate (wc) and various detergent extracts including sarkosyl-soluble (ss) and insoluble (si), and triton x-114 detergent phase (txd) and aqueous phase (txa) of the human isolate sp16 were examined by western blotting with serpulina ... | 1999 | 10659358 |
| motility-regulated mucin association of serpulina pilosicoli, the agent of colonic spirochetosis of humans and animals. | colonic spirochetosis is a disease of humans and animals characterized by colonization of the colonic mucus gel and intimate attachment of serpulina pilosicoli to the apical membrane of enterocytes. motility-regulated mucin association plays a key role in colonic infection by the related spirochete serpulina hyodysenteriae, the cause of swine dysentery. in this study the chemotaxis of serpulina pilosicoli porcine isolate p43/6/78, human isolate sp16, and canine isolate 16242-94 was examined by a ... | 1999 | 10659359 |
| coiling phagocytosis is the predominant mechanism for uptake of the colonic spirochetosis bacterium serpulina pilosicoli by human monocytes. | serpulina pilosicoli is a newly identified pathogenic spirochete that establishes persistent colonic infections in human beings and animals. macrophages are one of the key defenses against invasion of mucosal surfaces by bacterial pathogens. macrophages engulf many bacteria by conventional phagocytosis; however recent studies indicate coiling phagocytosis as a new and important mechanism for internalization of legionella pneumophila and spirochetes of the genus borrelia, leptospira, and treponem ... | 1999 | 10659360 |
| influences of diet and vaccination on colonisation of pigs by the intestinal spirochaete brachyspira (serpulina) pilosicoli. | the purpose of this study was to determine whether methods used to control swine dysentery (sd), caused by the intestinal spirochaete brachyspira (serpulina) hyodysenteriae, would also be effective in controlling porcine intestinal spirochaetosis (pis) caused by the related spirochaete brachyspira (serpulina) pilosicoli. weaner pigs in groups i (n=8) and ii (n=6) received a standard weaner pig diet based on wheat and lupins, whilst group iii (n=6) received an experimental diet based on cooked wh ... | 2000 | 10731619 |
| detectability and prevalence of brachyspira species in herds rearing health class feeder pigs in finland. | faeces samples were taken three times at two-week intervals, from the farrowing units of four herds of known brachyspira (formerly serpulina) status and one of unknown brachyspira status. brachyspira hyodysenteriae, brachyspira pilosicoli, brachyspira intermedia and brachyspira group iii were isolated from the faecal samples from the weaners in the herds using either a maximum of 50 ppm of olaquindox or no feed additives. the detection rates were relatively consistent. however, b hyodysenteriae ... | 2000 | 10777042 |
| prevalence of brachyspira species isolated from diarrhoeic pigs in brazil. | pathogenic intestinal spirochaetes of pigs include brachyspira (formerly serpulina) hyodysenteriae, the cause of swine dysentery, and brachyspira pilosicoli, the cause of porcine colonic spirochetosis (pcs). the purpose of this study was to assess the relative importance of brachyspira species in diarrhoeal disease of growing pigs on farms in southern brazil. the intensity and pattern of haemolysis, the production of indole and the hydrolysis of hippurate by reference and field porcine intestina ... | 2000 | 10791468 |
| identification of porcine intestinal spirochetes by pcr-restriction fragment length polymorphism analysis of ribosomal dna encoding 23s rrna. | the brachyspira (formerly serpulina) species rrl gene encoding 23s ribosomal rna (rrna) was used as a target for amplification of a 517bp dna fragment by polymerase chain reaction (pcr). the primers for pcr amplification had sequences that were conserved among brachyspira 23s rrna gene and were designed from nucleotide sequences of brachyspira hyodysenteriae, serpulina intermedia, brachyspira innocens and brachyspira pilosicoli available from the genbank database. digestion of pcr-generated prod ... | 2000 | 10889409 |
| cloning and dna sequence analysis of an immunogenic glucose-galactose mglb lipoprotein homologue from brachyspira pilosicoli, the agent of colonic spirochetosis. | colonic spirochetosis (cs) is a newly emerging infectious disease of humans and animals caused by the pathogenic spirochete brachyspira (formerly serpulina) pilosicoli. the purpose of this study was to characterize an antigen that was recognized by antibodies present in sera of challenge-exposed pigs. the gene encoding the antigen was identified by screening a plasmid library of human b. pilosicoli strain sp16 (atcc 49776) genomic dna with hyperimmune and convalescent swine sera. the predicted a ... | 2000 | 10899855 |
| detection of same sized 4.3 kb extrachromosomal dna elements in weakly beta-haemolytic human intestinal spirochaetes and serpulina pilosicoli of swine origin. | agarose gel electrophoresis of total dna from italian strains of weakly beta-haemolytic human intestinal spirochaetes (w beta his) and porcine serpulina pilosicoli reference strain p43/6/78 showed an extrachromosomal band having the same size and migrating at 4.3 kb. the same results were observed after agarose gel electrophoresis of dna obtained from the supernatant fluids of the spirochaetal cultures analysed. swine serpulina hyodysenteriae reference strain p18a was comparatively analysed and ... | 2000 | 10946400 |
| prevalence of intestinal pathogens in danish finishing pig herds. | our aim was to determine the prevalence of the intestinal bacteria: lawsonia intracellularis, brachyspira hyodysenteriae, serpulina intermedia, brachyspira innocens, brachyspira pilosicoli, pathogenic escherichia coli (serogroups o138, o139, o141 and o149) and salmonella enterica in danish finishing pig herds. a total of 79 herds was randomly selected and visited during 1998. from each herd, 20 faecal samples were collected from individual pigs weighing 30-50kg. furthermore, 10 pooled pen sample ... | 2000 | 10960714 |
| evaluation of blood culture systems for detection of the intestinal spirochaete brachyspira (serpulina) pilosicoli in human blood. | the anaerobic intestinal spirochaete brachyspira (serpulina) pilosicoli has been isolated from the bloodstream of french patients by manual blood culture systems. the purpose of this study was to determine whether the automated and manual blood culture systems used in australia are suitable for growth and detection of this organism. strains of b. pilosicoli were added to human blood to give concentrations ranging from 1 x 10(4) to 1 x 10(1) spirochaetes/ml and 10-ml volumes were inoculated into ... | 2000 | 11073157 |
| comparative prevalences of brachyspira aalborgi and brachyspira (serpulina) pilosicoli as etiologic agents of histologically identified intestinal spirochetosis in australia. | dna from gastrointestinal biopsy specimens from 28 australian patients with histologic evidence of intestinal spirochetosis (is) was subjected to pcrs to amplify segments of the 16s rrna and nadh oxidase genes of brachyspira aalborgi and brachyspira (serpulina) pilosicoli. b. aalborgi was identified in specimens from 24 (85.7%) patients and b. pilosicoli in those from 4 (14.3%) patients (2 of whom were also positive for b. aalborgi). for two patients, no product was amplified. this study demonst ... | 2001 | 11136797 |
| an investigation of the etiology of a mild diarrhea observed in a group of grower/finisher pigs. | an investigation into a mild diarrhea in a group of grower/finisher pigs was carried out in order to determine the etiology. a tiamulin injection and a carbadox-medicated ration were given to pens of pigs in a 2 x 2 factorial experimental design. pens of pigs were assessed a score, based on the consistency of the feces in the pen, each week. the clinical investigation looked for the intestinal pathogens brachyspira pilosicoli, b. hyodysenteriae, lawsonia intracellularis, salmonella spp., yersini ... | 2001 | 11195519 |
| pcr detection of brachyspira aalborgi and brachyspira pilosicoli in human faeces. | previously-developed pcr protocols specific for the 16s rrna gene of the intestinal spirochaetes brachyspira aalborgi and brachyspira pilosicoli were adapted for the detection of these species in human faeces, following dna extraction and purification using mini-prep columns. the limits of detection in seeded faeces for b. aalborgi and b. pilosicoli respectively were 2x10(2) and 7x10(3) cells per pcr reaction, equivalent to 5x10(4) and 1x10(5) cells per g of faeces. the pcr techniques were appli ... | 2001 | 11313130 |
| weakly beta-haemolytic human intestinal spirochaetes antagonize the haemolytic activity of clostridium perfringens alpha-toxin producer. | the production of haemolytic antagonism between weakly beta-haemolytic human intestinal spirochaetes (wbetahis) related to human intestinal spirochaetosis and clostridium perfringens alpha-toxin producer was investigated. a reduction of the clostridial haemolytic activity and a distortion of the haemolytic halo of clostridial alpha-toxin surrounded by a small zone of poorly cooperative haemolysis was clearly observed on the level of the spirochaetal growth area when 40 out of 41 wbetahis were cu ... | 2001 | 11346295 |
| survival of brachyspira hyodysenteriae and b. pilosicoli in terrestrial microcosms. | the survival of brachyspira hyodysenteriae and brachyspira pilosicoli was investigated at 10 degrees c in laboratory microcosms consisting of soil, porcine faeces, and in soil mixed with 10% porcine faeces, respectively. by plate spreading, survival of b. hyodysenteriae was found to be 10, 78 and 112 days in soil, soil mixed with 10% faeces, and in porcine faeces, respectively. the identities of the colonies on the plates were confirmed using pcr targeting 23s rdna for specific detection of b. h ... | 2001 | 11356316 |
| risk factors for intestinal pathogens in danish finishing pig herds. | the objective of this investigation was to identify risk factors for infection with the intestinal bacteria: lawsonia intracellularis, brachyspira hyodysenteriae, serpulina intermedia, brachyspira innocens, brachyspira pilosicoli and swine-pathogenic escherichia coli (serogroups o138, o139, o141 and o149) in danish finishing pig herds.a total of 79 herds was randomly selected and visited during 1998. from each herd, 20 faecal samples were collected from individual pigs weighing 30-50 kg. in tota ... | 2001 | 11448502 |
| diagnostic examination of human intestinal spirochetosis by fluorescent in situ hybridization for brachyspira aalborgi, brachyspira pilosicoli, and other species of the genus brachyspira (serpulina). | human intestinal spirochetosis, characterized by end-on attachment of densely packed spirochetes to the epithelial surface of the large intestines as a fringe has been associated with the weakly beta-hemolytic spirochetes brachyspira aalborgi and brachyspira (serpulina) pilosicoli. in this study, fluorescent in situ hybridization with oligonucleotide probes targeting 16s or 23s rrna of b. aalborgi, b. pilosicoli, and the genus brachyspira was applied to 40 sections of formalin-fixed, paraffin-em ... | 2001 | 11682538 |
| carriage of intestinal spirochaetes by humans: epidemiological data from western australia. | the purpose of this study was to investigate carriage of intestinal spirochaetes by selected population groups in western australia. stool specimens from 293 rural patients with gastrointestinal disorders, and from 227 healthy migrants from developing countries were cultured. spirochaete isolates were identified using pcr, and typed by pulsed field gel electrophoresis (pfge). brachyspira aalborgi was not isolated. brachyspira pilosicoli was recovered from 15 rural patients, all aboriginal. preva ... | 2001 | 11693517 |
| human intestinal spirochetosis: brachyspira aalborgi and/or brachyspira pilosicoli? | intestinal spirochetosis in humans (his) is a condition defined by the presence of a layer of spirochetes attached by one cell end to the colorectal epithelium. the pathologic significance of his is uncertain, but it has been linked to chronic diarrhea and other abdominal complaints. two anaerobic intestinal spirochete species have been associated with his, namely brachyspira pilosicoli and brachyspira aalborgi. brachyspira pilosicoli, which colonizes many animal species, is common (approximatel ... | 2001 | 11708739 |
| the search for brachyspira outer membrane proteins that interact with the host. | little is known about the outer membrane structure of brachyspira hyodysenteriae and brachyspira pilosicoli or the role of outer membrane proteins (omps) in host colonization and the development of disease. the isolation of outer membrane vesicles from b. hyodysenteriae has confirmed that cholesterol is a significant outer membrane constituent and that it may impart unique characteristics to the lipid bilayer structure, including a reduced density. unique proteins that have been identified in th ... | 2001 | 11708742 |
| comparative pathology and pathogenesis of naturally acquired and experimentally induced colonic spirochetosis. | research in the past decade has led to the recognition of brachyspira (formerly serpulina) pilosicoli as the primary etiologic agent of colonic spirochetosis (cs), an emerging cause of colitis in humans and animals. attachment of spirochetes to the epithelial surface of the lower intestine is considered to be the hallmark of cs. however, because b. pilosicoli, b. aalborgi and unclassified flagellated bacteria are found singly or together in humans and non-human primates with cs lesions, attachme ... | 2001 | 11708743 |
| porcine enteric spirochete infections in the uk: surveillance data and preliminary investigation of atypical isolates. | investigations into the possible causes of colitis and typhlocolitis were carried out on 98 pig units in the united kingdom between 1997 and 1999. brachyspira pilosicoli was identified most commonly, occurring as the suggested primary agent in 18% of the outbreaks but forming part of mixed infections in another 24% of outbreaks. the equivalent figures for other bacterial pathogens were: b. hyodysenteriae, 13% and 16%; lawsonia intracellularis, 10% and 15%: salmonella species, 6% and 12%; and yer ... | 2001 | 11708744 |
| eradication of endemic brachyspira pilosicoli infection from a farrowing herd: a case report. | brachyspira pilosicoli and b. innocens were isolated repeatedly from a herd of 60 sows which mostly produced feeder pigs but also raised some fattening pigs. postweaning diarrhea had been a severe problem in this herd for years. the b. pilosicoli eradication plan was based on the general guidelines for elimination of b. hyodysenteriae, with some modifications. the eradication measures were run in august 1997. in-feed medication with 200 p.p.m. tiamulin lasted for 18-30 days, depending on the age ... | 2001 | 11708747 |
| classification of brachyspira spp. isolated from swedish dogs. | brachyspira spp. were isolated from 21 of 32 sampled dogs (66%) in a colony of swedish beagle dogs with a history of diarrhea and from 3 of 17 swedish pet dogs (17%) with diarrhea. all swedish isolates were weakly beta-hemolytic and gave a negative indole reaction. eighty-eight percent showed negative alpha-galactosidase and hippurate reactions, but a positive beta-glucosidase reaction. two isolates were hippurate positive with a negative beta-glucosidase reaction. one additional german isolate ... | 2001 | 11708750 |
| brachyspira spp. (serpulina spp.) in birds: a review and results from a study of swedish game birds. | only limited data concerning the prevalence of intestinal spirochetes are available in game birds. this paper describes the prevalence and biochemical reactions of spirochetes isolated from 25 common partridges, 7 pheasants and 16 mallards originating from nine swedish game-bird farms. the birds, which had been submitted for post-mortem examination due to various problems, showed a variety of underlying diseases. additionally, fecal droppings from 22 common partridges, 20 pheasants and 20 mallar ... | 2001 | 11708752 |
| failure to detect brachyspira pilosicoli in bloodstream of australian patients. | | 2001 | 11712515 |
| prevalence of brachyspira pilosicoli in korean pigs, determined using a nested pcr. | | 2002 | 11878441 |
| first confirmation of porcine colonic spirochaetosis caused by brachyspira pilosicoli in iberian pigs in spain. | | 2002 | 11924554 |
| brachyspira (serpulina) pilosicoli of human origin interfere with the growth of clostridium perfringens alpha-toxin producer. | brachyspira (serpulina) pilosicoli of human origin interfere with the growth of clostridium perfringens alpha-toxin producer reducing the clostridial growth area and colonies number when bacteria were cultivated together in sheep blood agar plates. the growth inhibition of c. perfringens was only observed when b. (s.) pilosicoli grew 72-96 hours sooner than c. perfringens and after the inoculum of this latter the plates were anaerobically incubated for additional 48 hours. the phenomenon was obs ... | 2002 | 12019719 |
| brachyspira (serpulina) pilosicoli of human origin interfere with the haemolytic activity and the growth of staphylococcus aureus beta-toxin producer. | brachyspira (serpulina) pilosicoli related to intestinal spirochaetosis were found to interfere in vitro with the haemolytic activity and the growth of staphylococcus aureus beta-toxin producer. this interference was clearly appreciated because a reduction of the zone of the staphylococcal beta-toxin activity, the reduction and/or absence of cooperative haemolysis between bacteria, and the growth reduction of s. aureus were observed when b. (s.) pilosicoli were grown 72-96 hours sooner than s. a ... | 2002 | 12019720 |
| identification of brachyspira hyodysenteriae-specific dna fragments using representational difference analysis. | two novel brachyspira hyodysenteriae-specific dna fragments, designated as bh100 and bh400, were identified using representational difference analysis. to isolate the fragments the combined dna of the brachyspira pilosicoli, brachyspira intermedia, brachyspira murdochii and brachyspira innocens reference strains was subtracted from the genome of b. hyodysenteriae strain b204. both fragments were present in a single copy and mapped to different positions on the genome of b. hyodysenteriae b78(t). ... | 2002 | 12044671 |
| brachyspira (serpulina) pilosicoli spirochetemia in an immunocompromised patient. | the case of an elderly immunocompromised man with non-hodgkin's lymphoma who presented with fever, abdominal pain and bloody diarrhea is described. brachyspira pilosicoli was isolated from culture. the patient was treated with penicillin g i.v. and became afebrile. b. pilosicoli is a recently recognized enteric pathogen of humans and animals. intestinal spirochetosis should be included in the differential diagnosis of any immunocompromised or critically ill patient with dysentery. | 2002 | 12120947 |
| zinc bacitracin enhances colonization by the intestinal spirochaete brachyspira pilosicoli in experimentally infected layer hens. | brachyspira pilosicoli strain cpsp1 isolated from a chicken in a broiler breeder flock in queensland was used to experimentally infect 40 individually caged 22-week-old laying hens. another 10 birds were sham-inoculated with sterile broth. all chickens received a commercial layer diet, but 10 infected birds had 50 parts/10(6) zinc bacitracin (znb) incorporated in their food. birds were kept for 7 weeks, and faecal moisture, egg numbers, egg weights and body weights were recorded weekly. b. pilos ... | 2002 | 12396353 |
| evaluation of tiamulin and lincomycin for the treatment of broiler breeders experimentally infected with the intestinal spirochaete brachyspira pilosicoli. | brachyspira pilosicoli strain cpsp1 isolated from a chicken in a broiler breeder flock in queensland was used to experimentally infect 30 individually caged 22-week-old cobb 500 broiler breeder hens. another 10 birds were sham-inoculated with sterile broth. all birds failed to become colonized. at 29 weeks of age, all birds were transferred to a diet containing 50 parts/10(6) zinc bacitracin (znb) and were re-challenged with the same b. pilosicoli strain at 32 weeks of age, weekly for 5 weeks. t ... | 2002 | 12396354 |
| experimental infection of broiler breeder hens with the intestinal spirochaete brachyspira (serpulina) pilosicoli causes reduced egg production. | the pathogenic potential of the anaerobic intestinal spirochaetes brachyspira (serpulina) pilosicoli and brachyspira innocens was evaluated in adult chickens. thirty 17-week-old cobb broiler breeder hens were individually caged in three groups of 10 birds. control birds (group a) were sham inoculated with sterile broth medium. birds in the other two groups (groups b and c) were inoculated, respectively, with an isolate of b. innocens or of b. pilosicoli. birds were monitored daily, and killed at ... | 2002 | 12396362 |
| validation of an immunoperoxidase monolayer assay as a serologic test for porcine proliferative enteropathy. | the sensitivity and specificity of an immunoperoxidase monolayer assay (ipma) was evaluated in a blind serologic study of a group of disease-free pigs and a group of pigs experimentally infected with intestinal homogenate containing lawsonia intracellularis organisms. sixty pigs from the control group were kept in the source farm, and another 60 animals were transferred to an isolation unit aid challenged intragastrically. all animals were bled before and 21 days after challenge. fecal samples w ... | 2002 | 12423042 |
| increasing the viscosity of the intestinal contents stimulates proliferation of enterotoxigenic escherichia coli and brachyspira pilosicoli in weaner pigs. | the present study was designed to evaluate the effect of increased viscosity of the intestinal digesta on proliferation of enterotoxigenic escherichia coli and the intestinal spirochaete brachyspira pilosicoli in weaned pigs. pigs were fed an experimental diet based on cooked white rice (r), which was supplemented with carboxymethylcellulose (cmc; 40 g/kg diet) to increase digesta viscosity. thirty-six piglets weaned at 21 d of age were divided into six groups, three of which were fed r and thre ... | 2002 | 12425733 |
| diarrhoea in the growing pig - a comparison of clinical, morphological and microbial findings between animals from good and poor performance herds. | diarrhoea among growing pigs (8-13 weeks old) is a significant problem in many herds. nine herds with poor performance and diarrhoea among growing pigs were selected on the basis of their piglet mean age at a body weight of 25 kg, compared to the overall mean age in swedish herds. in addition, four herds with good average performance and no problems with diarrhoea were selected. pigs were necropsied and samples for histology and microbiology were collected. based on the necropsy findings, the pi ... | 2003 | 12589741 |
| detection by pcr and isolation assays of the anaerobic intestinal spirochete brachyspira aalborgi from the feces of captive nonhuman primates. | the purpose of this study was to investigate the presence of the anaerobic intestinal spirochetes brachyspira aalborgi and brachyspira pilosicoli in the feces of captive nonhuman primates (n = 35) from 19 species housed at the zoological gardens, perth, western australia. both spirochete species are known to infect human beings. dna was extracted from freshly collected feces with a commercially available qiaamp dna stool minikit and subjected to pcr protocols amplifying portions of the 16s rrna ... | 2003 | 12624050 |
| epidemiological studies of brachyspira pilosicoli in two australian piggeries. | the epidemiology of infection with the intestinal spirochaete brachyspira pilosicoli within pig herds is incompletely understood. to investigate this further, cross-sectional and cohort studies were undertaken on two piggeries. faeces were subjected to selective culture, and dna was extracted from growth on the primary media and amplified by polymerase chain reaction (pcr). on one farm, samples from other animal species and the environment were also examined. isolates were subjected to multilocu ... | 2003 | 12636999 |
| colonisation of pet shop puppies with brachyspira pilosicoli. | anaerobic intestinal spirochaetes of the genus brachyspira are known to colonise dogs, but relatively little is known about their prevalence, distribution or pathogenic potential. one species, brachyspira pilosicoli, is thought to cause diarrhoea in dogs, as well as in other animals and humans. to investigate the prevalence and distribution of infection, faecal samples from 49 puppies from six pet shops in the suburbs of perth, western australia were subjected to selective culture for anaerobic ... | 2003 | 12637005 |
| experimental infection of layer hens with a human isolate of brachyspira pilosicoli. | the anaerobic intestinal spirochaete brachyspira pilosicoli commonly colonizes the large intestine of a number of species, including chickens and human beings. the purpose of the current study was to determine whether an isolate of b. pilosicoli recovered from an hiv-infected patient with diarrhoea could infect and cause disease in adult chickens. over a 4-week period following experimental infection, a group of eight inoculated chickens showed a persistent and significant increase in faecal wat ... | 2003 | 12676876 |
| in vitro antimicrobial susceptibility of brachyspira pilosicoli isolates from humans. | the in vitro antimicrobial susceptibility of the anaerobic intestinal spirochete brachyspira pilosicoli was investigated by an agar dilution method. human (n = 123) and porcine (n = 16) isolates were susceptible to metronidazole, ceftriaxone, meropenem, tetracycline, moxifloxacin, and chloramphenicol; erythromycin and ciprofloxacin were not active. resistance to amoxicillin and clindamycin varied. amoxicillin susceptibility was restored by clavulanic acid. | 2003 | 12821498 |
| development of a duplex pcr assay for detection of brachyspira hyodysenteriae and brachyspira pilosicoli in pig feces. | a duplex pcr (d-pcr) amplifying portions of the brachyspira hyodysenteriae nadh oxidase gene and the b. pilosicoli 16s rrna gene was developed and then tested on dna extracted from 178 porcine fecal samples. the feces also underwent anaerobic culture and species-specific pcrs. fecal extraction-d-pcr detected seven additional samples containing b. hyodysenteriae and five more containing b. pilosicoli. | 2003 | 12843096 |
| clinical significance of human intestinal spirochetosis--a morphologic approach. | intestinal spirochetosis (is) is a condition defined morphologically by the presence of spirochetal microorganisms attached to the apical cell membrane of the colonic and rectal epithelium. intestinal spirochetes comprise a heterogeneous group of bacteria. in humans brachyspira aalborgi and brachyspira pilosicoli predominate. prevalence rates of is are low where living standards are high, in contrast to poorly developed areas where is is common. homosexuals and hiv-infected individuals are at hi ... | 2003 | 14556061 |
| molecular epidemiological study of brachyspira pilosicoli in finnish sow herds. | brachyspira (b.) pilosicoli, the causative agent of intestinal spirochaetosis in pigs, is a quite common laboratory finding from faecal samples of weaned and growing pigs in finland. a better understanding of the epidemiology of b. pilosicoli in and between finnish pig farms is needed. altogether 131 b. pilosicoli isolates from 49 finnish sow herds were studied by pulsed-field gel electrophoresis. mlui was used as a restriction enzyme for all the isolates, and smai for 70 isolates. the isolates ... | 2003 | 14596539 |
| colonic spirochetosis in children and adults. | we undertook a retrospective analysis of colonic spirochetosis in 14 cases: females, 3; males, 11; children, 4; adults, 10. two men had hiv infections. all children and both hiv-infected men had abdominal complaints, diarrhea, or both. most other adults underwent colonoscopy for polyp screening (n = 4) or follow-up of crohn disease (n = 1) or had other indications (n = 2) or diarrhea (n = 1). histologically, spirochetosis was identified in all parts of the colon and was not strongly associated w ... | 2003 | 14671970 |
| survival of intestinal spirochaete strains from chickens in the presence of disinfectants and in faeces held at different temperatures. | this study aimed to evaluate the efficacy of some commonly used disinfectants in inactivating the pathogenic avian intestinal spirochaetes brachyspira intermedia and brachyspira pilosicoli, and to examine spirochaete survival in chicken caecal faeces held at 4 degrees c, 25 degrees c or 37 degrees c. six disinfectants were evaluated at their recommended working concentrations: alkaline salts, quaternary ammonium, iodine as an iodophor, chlorine from a chlorine-release agent, glutaraldehyde and h ... | 2003 | 14676016 |
| [colonic spirochetes: an infrequent cause of adult diarrhea]. | large intestine infection due to spirochetes was described in 1967 and is mainly related to two pathogens, serpulina pilosicoli and brachyspira aalborgi. clinically, infection presents as diarrhea and/or rectorrhagia and is more frequent among homosexuals. its prevalence is difficult to estimate but significant differences have been described according to the socioeconomic level of the area studied. we describe three cases of diarrhea due to spirochetes, which are of interest due to the lack of ... | 2004 | 14718105 |
| colonization and risk factors for brachyspira aalborgi and brachyspira pilosicoli in humans and dogs on tea estates in assam, india. | the prevalence of colonization with the anaerobic intestinal spirochaetes brachyspira aalborgi and brachyspira pilosicoli was investigated in humans (n = 316) and dogs (n = 101) living on three tea estates in assam, india. colonization was detected using pcr on dna from faeces. nineteen (6%) human faecal samples contained b. aalborgi dna, 80 (25.3%) contained b. pilosicoli dna, and 10 (3.2%) contained dna from both species. one canine sample contained dna from b. pilosicoli. significant factors ... | 2004 | 14979599 |
| immunomagnetic separation of the intestinal spirochaetes brachyspira pilosicoli and brachyspira hyodysenteriae from porcine faeces. | porcine intestinal spirochaetes are fastidious anaerobic organisms and, as a consequence, it has been necessary to develop various protocols to enhance their isolation from or detection in faeces. immunomagnetic separation (ims) is a method developed recently to improve separation of target cells from mixed cell suspensions. the purpose of the present study was to compare the relative sensitivity of ims for isolation of brachyspira pilosicoli and brachyspira hyodysenteriae with current routine d ... | 2004 | 15017286 |
| extensive intestinal spirochaetosis in pigs challenged with brachyspira pilosicoli. | a field isolate of brachyspira pilosicoli, the aetiological agent of porcine intestinal spirochaetosis, was inoculated by stomach tube into six 6-week-old pigs. all animals developed loose to watery faeces and were killed successively on days 8, 14 or 17 post-inoculation (day 17 being the end of the study). mild mucosal reddening and flecks of pus characterized the gross lesions, while diffuse, catarrhal colitis was revealed microscopically in all animals. intestinal spirochaetosis with moderate ... | 2004 | 15017287 |
| brachyspira pilosicoli colonization in experimentally infected mice can be facilitated by dietary manipulation. | the purpose of this study was to determine whether defined dietary manipulations would enhance colonization of mice experimentally challenged with the intestinal spirochaete brachyspira pilosicoli. weanling c3h/hej mice (n = 48) were fed either a standard balanced mouse diet or a diet supplemented with 50 p.p.m. zinc bacitracin (znb), with 50 % (w/w) lactose or with both supplements. eight mice from each group were challenged orally with a human strain of b. pilosicoli (wesb), whilst four in eac ... | 2004 | 15017288 |
| biochemical properties of membrane-associated proteases of brachyspira pilosicoli isolated from humans with intestinal disorders. | a membrane-associated, subtilisin-like, serine protease activity was found in both pathogenic and non-pathogenic strains of brachyspira species in a previous study, but the biochemical properties of the enzyme were not investigated. the purpose of the present study was to characterize further the biochemical properties, including substrate specificity, of the membrane-associated protease of brachyspira pilosicoli isolated from humans with intestinal disorders. protease activity of detergent-enri ... | 2004 | 15017289 |
| prevalence, risk factors and molecular epidemiology of brachyspira pilosicoli in humans on the island of bali, indonesia. | the purpose of this study was to investigate the prevalence and epidemiology of the anaerobic intestinal spirochaete brachyspira pilosicoli amongst indonesians living in rural and urban settings on the island of bali. faecal samples (n = 992) were collected on two occasions, 4 months apart, from people living in four traditional farming villages, one peri-urban location and one urban area. samples were cultured anaerobically on selective agar and intestinal spirochaete isolates were confirmed to ... | 2004 | 15017290 |
| analysis of genetic variation in brachyspira aalborgi and related spirochaetes determined by partial sequencing of the 16s rrna and nadh oxidase genes. | the purpose of this study was to investigate genetic variation in the anaerobic intestinal spirochaete brachyspira aalborgi by partial sequencing of the 16s rrna and nadh oxidase genes. the spirochaete is poorly cultivable; hence, only six isolates were available for analysis. additional sequences were amplified from dna extracted from fixed colorectal biopsies from 26 patients with histological evidence of intestinal spirochaetosis, and from the faeces of six non-human primates (nhp). multiple ... | 2004 | 15017291 |