| pharmacological and pathological studies on hepatic protective crude drugs from taiwan (v): the effects of bombax malabarica and scutellaria rivularis. | bombax malabarica dc and scutellaria rivularis b. were extracted in boiling water and concentrated into 1g/ml solution to investigate their hepatoprotective effect. carbon tertrachloride (ccl4) was injected into rat subcutaneously with a dose of 3.0 ml/kg to induce experimental acute hepatotoxicity in the animal. the activities of serum glumtamic-oxaloacetic transaminase (sgot) and serum glutamicpyruvic transaminase (sgpt) were measured after 72 hours of ccl4 administration. the hepatopathologic ... | 1992 | 1471609 |
| anatomical and histological studies of bupleuri radix. | three kinds of bupleuri radix were found in taiwan. to clarify their origins, histological studies were carried out to identify their internal and external structures. it was found that bupleuri radix or "chai-hu" of taiwan is derived from roots of the following species" 1, bupleurum chinense which was imported from china, 2, bupleurum falcatum var. komarowi a cultivated production of japanese origin, and 3, bupleurum kaoi which is indigenous to taiwan. these bupleuri radix were easily assigned ... | 1991 | 1767798 |
| [dynamic variation of saikosaponin contents]. | in this paper both the cultivated and wild bupleurum chinense and b. scorzonerifolium from northeast china of different growing periods and ages were analyzed for the contents of saikosaponins a and d by hplc and total saikosaponins by spectrophotometry. the results indicated that the saikosaponin content of b. chinense was higher than that of b. scorzonerifolium, and that the saikosaponin contents of the cultivated species were higher than or almost equal to those of the wild ones. it was also ... | 1991 | 1804165 |
| the pharmacological and pathological studies on taiwan folk medicine (vi): the effects of elephantopus scaber subsp. oblanceolata, e. mollis and pseudoelephantopus spicatus. | the liver protective effect of elephantopus scaber subsp. oblanceolata, e. mollis and pseudoelephantopus spicatus on ccl4 induced hepatotoxicity has been determined by liver enzyme functional test and pathological studies. as the result indicated, the acute increase of serum transaminase (sgot and sgpt level which was caused by ccl4 administration (3.0ml/kg, s.c.) can be significantly reduced when treating with e. scaber subsp. oblanceolata (p less than 0.001), e. mollis (p less than 0.01), p. s ... | 1991 | 1897489 |
| the pharmacological and pathological studies on taiwan folk medicine (iii): the effects of bupleurum kaoi and cultivated bupleurum falcatum var. komarowi. | the protective effects of water extract from roots of bupleurum kaoi liu, chao et chuang and bupleurum flacatum l. var. komarowi koso-polj on ccl4-induced hepatoxicity have been investigated. both b. kaoi (p less than 0.05) and b. falcatum var. komarowi (p less than 0.01) possessed more marked anti-hepatotoxic pharmacological effects than bupleurum chinense dc., the typical strain widely used in taiwan. the pathological improvement from treatment by means of the three drugs to alleviate ccl4-ind ... | 1990 | 2270844 |
| the pharmacological and pathological studies on taiwan folk medicine (iv): the effects of echinops grijisii and e. latifolius. | the protective effect of "san-fang-feng" derived from the root of echinops grijsii hance on ccl4-induced hepatotoxicity has been studied. this crude drug was found to possess a marked hepatoprotective effect. a comparison of the protective effect with "lou-lu" derived from the root of e. latifolius tausch and "pei-chai-hu" original bupleurum chinense showed that e. grijisii and e. latifolius were more effective than b. chinense as reported previously. the results suggested that both e. grijisii ... | 1990 | 2270845 |
| [preliminary study of traditional chinese medicine treatment of minimal brain dysfunction: analysis of 100 cases]. | this paper reports the clinical treatment of 100 patients with minimal brain dysfunction (mbd), their age ranged from 7-14.2 years, and the average age was 10.5. patients were divided into tcm and wm group randomly. the tcm group (80 cases) were treated with the therapy of subdueing hyperactivity of the liver and invigorating the function of the spleen by using: bupleurum chinense, scutellaria baicalensis, astragalus membranaceus, codonopsis pilosula, ligustrum lucidum, lophatherum gracile and t ... | 1990 | 2397543 |
| [comparison on saikosaponin levels in the root of bupleurum chinense of various sizes]. | | 1985 | 2933158 |
| the pharmacological and pathological studies on several hepatic protective crude drugs from taiwan (i). | this study is to investigate the hepatic protective effect of several taiwan crude drug extractions on the carbon tetrachloride (ccl4)-induced hepatotoxicity in rats. the pharmacological and pathological effects of bupleurum chinense, phellodendron wilsonii, clematis chinensis and hedyotis corymbosa were analyzed by liver enzyme function test and pathological studies. however, the results of amine transferase sgot and sgpt have shown a significant hepatic protective effect after treatment with b ... | 1988 | 3245533 |
| [comparative studies on the roots of wild and cultured bupleurum chinense dc]. | | 1988 | 3264223 |
| hepatoprotective effects of taiwan folk medicine: ixeris chinensis (thunb.) nak. on experimental liver injuries. | the hepatoprotective effects of ixeris chinensis (thunb.) nak. were studied on acute hepatitis induced in mice by a single dose of carbon tetrachloride (31.25 microliters/kg, ip) or acetaminophen (600 mg/kg, ip), and in rats by a single dose of beta-d-galactosamine (188 mg/kg, ip). hepatoprotective activity was monitored by estimating the serum transaminases (sgot and sgpt) levels and histopathological changes in the livers of experimental animals. the ixeris chinensis (thunb.) nak. extracts sig ... | 1994 | 7872236 |
| hepatoprotective effects of taiwan folk medicine: wedelia chinensis on three hepatotoxin-induced hepatotoxicity. | the hepatoprotective effects of a taiwanese crude herb, hwang-hua-mih-tsay (wedelia chinensis (osbeck) merr.), were investigated. acute hepatitis was induced by three hepatotoxins: carbon tetrachloride and acetaminophen in mice, and d(+)-galactosamine in rats. after treatment with w. chinensis (300 mg/kg, p.o.) at 2, 6 and 10 hours, a reduction in the elevation of serum glutamate oxaloacetic transaminase (sgot) and glutamate pyruvic transaminase (sgpt) levels was observed at 24 hrs after hepatot ... | 1994 | 7992815 |
| the protective effect of alstonia scholaris r. br. on hepatotoxin-induced acute liver damage. | the hepatoprotective effect of alstonia scholaris r. br. on liver injuries induced by carbon tetrachloride (ccl4). beta-d-galactosamine, acetaminophen and ethanol were investigated by means of serum-biochemical and histopathological examinations. post treatment of a scholaris reduced dose-dependently the elevation of serum transaminases level and histopathological changes such as cell necrosis, inflammatory cell infiltration, which were caused by the single administration of 32 microliters/kg cc ... | 1996 | 8874672 |
| the hepatoprotective effects of taiwan folk medicine ham-hong-chho in rats. | bidens pilosa l. var minor (blume) sherff, b. pilosa l. and b. chilensis dc (compositae), commonly known as "ham-hong-chho" in taiwan, have been traditionally used for medicinal purposes. to clarify and compare the hepatoprotective effects of these three plants, we evaluated their potential effectiveness on ccl4- and acetaminophen-induced acute hepatic lesions in rats. the results indicated that the increase in sgot and sgpt activities caused by ccl4 (3.0 ml/kg, s.c.) and acetaminophen administr ... | 1996 | 8982435 |
| analysis of mitogenic substances in bupleurum chinense by esr spectroscopy. | the polyphenolic substance(s) in the hot water extract of bupleurum chinense (psf) showed strong mitogenic activity. in this paper, we analyzed psf by using esr spectroscopy, and found that i) psf showed a strong esr signal on g = 2.005 which was similar to the commercially available lignin; ii) sho-saiko-to, which contains an extract of b. chinense, also showed similar signals on esr; iii) powdered b. chinense also showed similar signals on g = 2.005. peroxidase activity, essential for producin ... | 1997 | 9013818 |
| wide range of molecular weight distribution of mitogenic substance(s) in the hot water extract of a chinese herbal medicine, bupleurum chinense. | in this study, we examined the contribution of lignin-like materials in lower molecular weight (mw) fractions from the hot water extract of bupleuri radix (bupleurum chinense) (hwe-br) for their immunopharmacological activities. mitogenic activity was detected in all the fractions of mw ranges: lower than 1.0 kda, 1.0-3.5 kda, 3.5-10 kda, and 10-50 kda. after naclo2 treatment of these subfractions, uv spectra, esr spectra, mitogenic activities on murine b-cells, and the activity of inducing nitr ... | 1997 | 9255416 |
| saikosaponin v-1 from roots of bupleurum chinense dc. | three triterpenoidal saponins, saikosaponin v-1(1), 6"-o-acetyl-saikosaponin b2 (2) and 6"-o-acetyl-saikosaponin d(3) were isolated from the roots of the title plant and the structures were identified on the basis of spectral analysis. saikosaponin v-1 is a new compound, which was identified as 3beta,16alpha,23,28-tetrahydroxy-olean-11,13(18)-dien-30-oic acid-3-o-beta-d-glucopyranosyl-(1-->3)-beta-d-fucopyranosyl-30-o-xylitol ester. | 2001 | 11407813 |
| [a new saikosaponin from bupleurum chinense dc]. | bupleurum chinense dc. is a well-known and very important traditional chinese drug. it is often used to treat common cold with fever, alternating chill and fever, feeling of fullness and oppression in the chest. a new saikosaponin was isolated from bupleurum chinense dc. and its structure was identified as 3-o-[beta-d-glucopyranosyl (1-->3)-beta-d-fucopyranosyl]-3 beta, 16 alpha, 23, 28-tetrahydroxy-olean-11, 13 (18)-dien-30-oic acid 30-o-[xylityl(1-->1)]-beta-d-glucopyranosyl-6-ester on the bas ... | 1998 | 11939068 |
| [studies on technology of supercritical-co2 fluid extraction for volatile oils and saikosaponins in bupleurum chinense dc]. | to study the technology of supercritical-co2 fluid extraction (sfe-co2) for the volatile oils and saikosaponins in bupleurum chinense. | 2000 | 12212097 |
| [study on organogenesis and dry substance accumulation of bupleurum chinense]. | to learn the development characteristics of bupleurum chinese dc. and to provide the cultivation fundament. | 2003 | 14650055 |
| [plant growth analysis of bupleurum chinense]. | to learn the development characteristics of bupleurum chinense dc. for the cultivation fundament. | 2003 | 14692316 |
| [rapd analysis of genuiness on source of bupleurum chinense]. | to identify the genuiness on 5 samples of bupleurum chinense dc. from cultivating base in dongfeng county, jilin province. | 2003 | 15058203 |
| [the influence of seed maturity and seed coat on the germination of bupleurum chinense and b. falcatum]. | to investigate the factors influencing the seed germination of bupleurum chinense and b. falcatum. | 2003 | 15139103 |
| preventive effect of traditional herbal formulae against experimental hypercholesterolemia in rats with special reference to blood lipoprotein cholesterol levels. | to investigate the effect of traditional herbal formulae (zhong yao) against experimental hypercholesterolemia, we assayed serum lipoprotein levels and hepatic function in rats administered herbal formulae under cholesterol rich diet. two kinds of herbal formulae; prescription 1 (bupleurum chinense and other 9 kinds of herbs) and 2 (camellia sinensis and other 5 kinds of herbs) were prepared. all of 35 rats were separated into 4 groups, group p fed under cholesterol rich diet for 15 days and adm ... | 2004 | 15325730 |
| [study on liposome preparation technique of bupleurum chinense volatile oil for injection]. | to study the preparation technique of the liposome of bupleurum chinense volatile oil. | 2004 | 15706913 |
| in vitro anti-helicobacter pylori action of 30 chinese herbal medicines used to treat ulcer diseases. | infection by helicobacter pylori has been ascertained to be an important etiologic impetus leading usually to chronic active gastritis and gastric ulcer with growing incidences worldwide. utilizing as the test pathogen a standard and five clinic strains of helicobacter pylori, the antibacterial action was assessed in vitro with ethanol extracts of 30 chinese herbal medicines which have been frequently prescribed since ancient times for treating gastritis-like disorders. among the 30 tested mater ... | 2005 | 15814268 |
| haemolytic activities and adjuvant effect of bupleurum chinense saponins on the immune responses to ovalbumin in mice. | in this study, the haemolytic activities of bupleurum chinense saponins (bcs) and its adjuvant potentials on the immune responses of icr mice against ovalbumin (ova) were evaluated. bcs showed a slight haemolytic effect, with its haemolytic percents being 3.32% and 1.19% at the concentrations of 500 and 250 microg/ml, respectively. icr mice were immunized subcutaneously with ova 100 microg alone or with ova 100 microg dissolved in saline containing aluminium hydroxide gel (alum, 200 microg), qui ... | 2006 | 16214270 |
| analysis of the fatty acid from bupleurum chinense dc in china by gc-ms and gc-fid. | fatty acid of the root of the medicinal plant of bupleurum chinense dc in china has been investigated by gas chromatography combined with mass spectroscopy. after methyl-esterification, eight fatty acid compositions were identified by gc-ms. a simple and rapid determination of the fatty acid has been firstly developed by gc-fid. the derivatization condition was investigated in order to validate this method. palmitic acid, palmitoleic acid, oleic acid and linoleic acid were analyzed simultaneousl ... | 2005 | 16327203 |
| [identification of the fumatory radix bupleuri with sulfur by static headspace gc-ms]. | to identify whether radix bupleuri (bupleurum chinense) was fumed with sulfur. | 2005 | 16494020 |
| [effect of saikosaponins on epileptic seizure and eeg in pentetrazole-induced chronic kindling rats]. | to study the effect of saikosaponins, the active ingredients of bupleurum chinense dc, on epileptic seizure and eeg of pentetrazole (ptz)-induced chronic kindling rats. | 2006 | 16503523 |
| [studies on fingerprints of bupleurum chinense from hebei province with hplc-uv]. | to establish a hplc fingerprint analysis method for identification of bupleurum chinense of hebei province and compare the fingerprints of radix bupleuri collected from different habitats so as to establish a sensitive and specific method for controlling the quality of radix bupleuri. | 2006 | 16617777 |
| [effect of saikosaponins on glial fibrillary acidic protein expression in hippocampal astrocytes of pentetrazole-induced chronic kindling rats]. | to study the effect of saikosaponins, the active ingredients of bupleurum chinense dc, on glial fibrillary acidic protein (gfap) expression in hippocampal astrocytes of chronic kindling rats induced by pentetrazole (ptz). | 2006 | 16624750 |
| [saikosaponins inhibit increased glutamate and gaba expressions in the hippocampus of pentetrazole-induced slow kindling rats]. | to study the effect of saikosaponins, the active ingredients of bupleurum chinense dc. on glutamate and gaba expressions in the hippocampus of slow kindling rats induced by pentetrazole. | 2006 | 16939901 |
| [preliminary study on the characteristics of bupleurum chinense in nutrition uptake]. | it was studied that the characteristics of bupleurum chinense dc. in nutrition uptake based on potted and field experiment to provide a properly fertilizer application measure. the result showed that the absorption amounts of n, p and k were in the order of k > n > p under field condition. for one-year-old b. chinesnse dc., the average n, p2o5 and k2o absorption per 100 kg dry matter of root were 11.77 kg, 2.43 kg, 14.07 kg respectively; for two-year-old, the average n, p2o5 and k2o absorption a ... | 2006 | 17039870 |
| [preliminary study on n, p, k fertilizer to control of root rot of bupleurum chinense]. | the application of n and k fertillizer could improve the sensibility of bupleurvum chinense dc. to root rot, while large application of p fertilizer could decrase the sensibility. the fertilizer measure which could obtain highest yield but could not increase its disease resistense. to protect bupleurum chinense against root rot, more phosphorous fertilizer, certain nitrogen and potassium ferilizer should be applied in early elongation stage in the second growing year. | 2006 | 17326397 |
| its sequence analysis used for molecular identification of the bupleurum species from northwestern china. | radix bupleuri (chaihu), a famous traditional chinese medicine, is derived from the dried roots of bupleurum chinense dc. and b. scorzonerifolium willd. but nowadays, other nine species and varieties from genus bupleurum are also used as radix bupleuri in northwestern china. the authentic identification of dried roots of b. chinense and b. scorzonerifolium, however, is difficult to just base on the appearance and morphology. a molecular genetic method was developed to help discriminate the origi ... | 2007 | 17509842 |
| [histochemecal localization and the content compare of main medicinal components of vegetative organs in bupleurum chinense dc]. | the paraffin sectioning, histochemistry and phytochemistry methods were applied in the study of the localization and the content changes of saponins and flavonoids in the vegetative organs in bupleurum chinense dc. the results showed that the saponins distributed in vascular cambium and secondary phloem of the root; in the stem, they distributed mainly in epiderm,collenchyma and the epithelial cells of the secretory canals which lied in cortex and pith; in the leaf, they distributed in the epide ... | 2007 | 17966458 |
| elucidation of danzhixiaoyao wan and its constituent herbs on antioxidant activity and inhibition of nitric oxide production. | danzhixiaoyao wan (dw) is a common 10 herbs formulation in china for regulating several clinical conditions affecting women. this research tried to explain one of dw's functions, purging heat, using in vitro pharmacological analyses. the whole formulation and each single herb of dw were compared based on antioxidant activity with the oxygen radical absorbance capacity (orac) assay, and for their inhibitory effect (ie) on nitric oxide (no) production by lipopolysaccharide (lps)-activated raw 264. ... | 2007 | 18227909 |
| [determination of content of saikoside in bupleurum chinense from different sources]. | to determine the contents of saikosaponin a, d and saikosaponins in radix bupleuri from different and assess their quality. | 2008 | 18536454 |
| ultrastructure and secretion of secretory canals in vegetative organs of bupleurum chinense dc. | the development of secretory canals in vegetative organs of bupleurum chinense dc. and the accumulation of essential oils were investigated by transmission electron microscopy (tem) analysis. the secretion mechanism of the essential oil was also discussed. the results indicate that the plastid, ground substances of cytoplasm and mitochondria took part in the biosynthesis of oil or oil precursor. the endoplasmic reticulum involved in the transport of essential oil to the secretory lumen. at latte ... | 2008 | 18567500 |
| localization and dynamic change of saikosaponin in root of bupleurum chinense. | anatomical, histochemical and phytochemical methods were used to investigate the structure, the localization and content changes of total saikosaponin and saikosaponin-a of the roots of bupleurum chinense dc. at different developmental stages. results showed that saikosaponin was mainly distributed in pericycle and primary phloem in the young root; but in the mature root, it was mainly distributed in vascular cambium and secondary phloem. during the whole growth period from the pre-blossom, blos ... | 2008 | 18713344 |
| [studies on fingerprints of bupleurum chinense and vinegar-baked bupleurum chinense from hubei province]. | to establish the hplc fingerprint of bupleurum chinense from gap provenance of hubei province baokang, and discuss the changes before and after processing. | 2008 | 18826138 |
| new megastigmane sesquiterpene and indole alkaloid glucosides from the aerial parts of bupleurum chinense dc. | a new megastigmane sesquiterpene glucoside named chaihuxinoside a (1), and a new indole diglucoside named chaihuxinoside b (9) were isolated from the aerial parts of bupleurum chinense dc. along with eight known compounds. structures of two new compounds were elucidated by a combination of chemical and spectroscopic methods. chaihuxinosides a and b were characterized as 11-hydroxyl-4-en-3,9-dioxo- megastigmane-11-o-beta-d-glucopyranoside (1), and 3-carboxy-indole-10-o-beta-d-glucopyranosyl(1-->2 ... | 2009 | 18930790 |
| [professor ling changquan's experience in treating primary liver cancer: an analysis of herbal medication]. | on the basis of outpatients' medical records concerning primary liver cancer (plc), data of 552 patients (with 2020 effective prescriptions) from the outpatient department of changhai hospital treated by professor ling changquan were collected. the nature, flavor and meridian distribution of the herbs used in the prescriptions were summarized by frequency method, and the features of the herbs used according to syndrome differentiation were analyzed by logistic regression. the couple herbs used w ... | 2008 | 19063833 |
| evaluation of traditional chinese herbal medicine: chaihu (bupleuri radix) by both high-performance liquid chromatographic and high-performance thin-layer chromatographic fingerprint and chemometric analysis. | chaihu (bupleuri radix), roots of bupleurum chinense and b. scorzonerifolium, is an authentic chinese materia medica in the chinese pharmacopoeia. some other species such as the roots of b. falcatum, b.bicaule and b. marginatum var. stenophyllum similar to chaihu can also be occasionally found in local raw herb markets. the quality of 33 lots of authenticated chaihu samples vs. 31 lots of commercial samples was evaluated by both high-performance liquid chromatography-evaporative light scattering ... | 2009 | 19084233 |
| [determination of ferulic acid in fuan oral liquid by high-performance capillary electrophoresis]. | to establish a quality control method of fuan oral liquid. | 2009 | 19304552 |
| [studies on the chemical constituents of the aerial part of bupleurum chinense (ii)]. | to study the chemical constituents of the aerial part of bupleurum chinense. | 2009 | 19565712 |
| herbal formula syjn protect pc12 cells from neurotoxicity induced by corticosterone. | syjn is a chinese herbal formula, containing four herbs: bupleurum chinense dc., curcuma aromatica salisb., perilla frutescens (linn.) britt. and acorus tatarinowii schott. previous studies on the formula in our laboratory revealed an antidepressant-like effect on animal models of behavioral despair. however,the mechanisms underlying such antidepressant-like effect are yet to be understood. the aim of this work was to verify the previously established antidepressant-like effects on cell level us ... | 2009 | 19635548 |
| drug-use pattern of chinese herbal medicines in insomnia: a 4-year survey in taiwan. | insomnia is a common complaint in the general population. interest in the use of alternative treatments for insomnia is increasing exponentially and is fairly common in taiwan. we undertook a survey to define the drug utilization patterns of chinese herbal medicines (cm) for insomnia in taiwan. | 2009 | 19753680 |
| [comparative research of different bupleurum chinense composition to influence of hepatotoxicity of rats and oxidative damage mechanism]. | to compare the influence of different bupleurun chinense composition to the degree of hepatotoxicity damage to rats and oxidative damage mechanism. | 2009 | 20030090 |
| metabolomics study on the anti-depression effect of xiaoyaosan on rat model of chronic unpredictable mild stress. | ethnopharmacology: xiaoyaosan, a famous chinese prescription, composed of poria (poria cocos (schw.) wolf), radix paeoniae alba (paeonia lactiflora pall.), radix glycyrrhizae (glycyrrhiza uralensis fisch.), radix bupleuri (bupleurum chinense dc.), radix angelicae sinensis (angelica sinensis (oliv.) diels), rhizoma atractylodis macrocephalae (atractylodes macrocephala koidz.), herba menthae (mentha haplocalyx briq.), and rhizoma zingiberis recens (zingiber officinale rosc.), has been widely used ... | 2010 | 20079416 |
| effects of syjn, a chinese herbal formula, on chronic unpredictable stress-induced changes in behavior and brain bdnf in rats. | suyu-jiaonang (syjn) is a chinese herbal formula that contains four herbs: bupleurum chinense dc, curcuma aromatica salisb., perilla frutescens (linn.) britt., and acorus tatarinowii schott. previous studies conducted in our laboratory have revealed an antidepressant-like effect of the formula in various mouse models of behavioral despair. the present study aimed to investigate whether syjn could produce antidepressant-like effects in chronic unpredictable stress (cus)-induced depression model i ... | 2010 | 20138132 |
| [differences of saikosaponin contents in bupleurum chinense from habitats in dongling mountain & wuling mountain of beijing]. | the different growing habitats of bupleurum chinense were investigated in donglin mountain & wuling mountain areas, the saikosaponin a and d in samples of b. chinense collected from different habitats were determined by hplc. results showed that b. chinense distributed in various habitats, such as meadow, understory and brushy. significant differences of saikosaponin contents were observed. the higher saikosaponins contents were showed in samples from meadow habitats, while the lower saikosaponi ... | 2009 | 20352996 |
| herbal formula syjn increases neurotrophin-3 and nerve growth factor expression in brain regions of rats exposed to chronic unpredictable stress. | syjn is a chinese herbal formula that contains four herbs: bupleurum chinense dc., curcuma aromatica salisb., perilla frutescens (l.) britt., and acorus tatarinowii schott. previous studies conducted in our laboratory have revealed an antidepressant-like effect of the formula in chronic unpredictable stress (cus)-induced depression model in rats. the present study aimed to investigate whether neurotrophin-3 (nt-3) and nerve growth factor (ngf) are involved in the antidepressant-like action of sy ... | 2010 | 20600769 |
| [breeding of new varieties "zhongchai no. 2" and "zhongchai no. 3" of bupleurum chinense]. | to breed new varieties with better uniformity and commercial quality as well as higher saikosaponin contents. | 2010 | 20931839 |
| [study on main inheritable parameters of test-tube plants population of bupleurum chinense in different growing periods]. | to determining the growth status and medicine quality of test-tube plants of bupleurum chinense. | 2010 | 21049603 |
| ["dose-toxicity" relationship study on rat's chronic hepatoxicity of refined products of saikosaponin by alcohol elution]. | to observe the degree of hepatoxicity damage to rats after taking different dose refined products of saikosaponin by alcohol elution for 15 days. | 2010 | 21137351 |
| in vivo evaluation of anthelmintic potential of medicinal plant extracts against dactylogyrus intermedius (monogenea) in goldfish (carassius auratus). | in the present study, an attempt has been made to petroleum ether, chloroform, ethyl acetate, methanol, and water extracts of prunus amygdalus batsch seeds (semen amygdali), cimicifuga foetida l. rhizomes (rhizoma cimicifugae), peucedanum decursivum (miq.) maxim roots (radix peucedani), momordica cochinchinensis spreng. seeds (semen momordicae), and bupleurum chinense dc. roots (radix bupleuri chinensis) for their in vivo anthelmintic activity against monogenean dactylogyrus intermedius in goldf ... | 2010 | 21153837 |
| validation and application of reference genes for quantitative gene expression analyses in various tissues of bupleurum chinense. | it is crucial to select stable references in gene expression analyses using quantitative real-time pcr (qrt-pcr). in this work, seven frequently used reference genes, 18s, actin, ef1α, α-tubulin, β-tubulin, cyclophilin and cytoplasmic ribosomal protein l2 (l2), from bupleurum chinense dc. were evaluated as the internal control in five tissues, roots, stems, leaves, flowers and fruits, before tissue specific gene expression assays. the results showed that β-tubulin was the most stable and reliabl ... | 2010 | 21161394 |
| characterization of the multiple absorbed constituents in rats after oral administration of chai-huang decoction by liquid chromatography coupled with electrospray-ionization mass spectrometry. | a rapid, sensitive, and specific method by high-performance liquid chromatography (hplc) coupled to diode-array detection (dad) and tandem mass spectrometry (ms) techniques was developed for the identification of absorbed constituents and their metabolites in rats after the oral administration of a chai-huang decoction (chd), which consists of bupleurum chinense and scutellaria baicalensis in the proportion 1 : 1 (w/w). by comparing their retention times and ms data with those of authentic compo ... | 2010 | 21162005 |
| anti-diabetic effects of a coptis chinensis containing new traditional chinese medicine formula in type 2 diabetic rats. | the chinese formula tang-min-ling (tml), an improved product of the decoction of dachaihu which has a history of more than 2000 years, has main constituents of coptis chinensis franch, scutellaria baicalensis georgi, rheum officinale baill and bupleurum chinense dc. a multi-central randomized controlled investigation performed previously by us has showed that tml has positive effects on regulating glycometabolism in type 2 diabetes (t2dm) patients, but the mechanisms remain unclear. using otsuka ... | 2011 | 21213398 |
| [study on dynamic accumulation of index components from bupleurum chinense in various collecting periods]. | to study the dynamic change law of volatile oil, saikosaponin a, d and alcohol-extract from bupleurum chinense at songxian region in henan province, and to explore the optimal harvest period of bupleurum chinense. | 2010 | 21213529 |
| [construction of genetic linkage map of bupleurum chinense dc. using issr and ssr markers]. | abstract: molecular genetic map is a fundamental organizational tool for genomic research. however, a genetic linkage map for bupleurum chinense dc. has not been developed. in this study, with the theory of pseudo-testcross, 96 f1 plants from an intraspecific cross of b. chinense were used as mapping populations. twenty eight issr (inter-simple sequence repeat) primers and 44 ssr (simple sequence repeat) primers were used to detect the polymorphisms between the parental plants, and of them, 28 i ... | 2010 | 21355221 |
| [characteristics and abortion cytological pathways of male sterility of bupleurum chinense]. | to study the characteristics of male sterility of bupleurum chinense and further explore the developmental period and reason of abortion for the male sterile plants. | 2010 | 21438382 |
| [cropping patterns of bupleurum chinense in semi-arid region of loess plateau]. | field experiments were conducted to investigate the suitable cropping patterns of grain crops and medical plant bupleurum chinense in the semi-arid region of the loess plateau. three cropping patterns were designed, including corn-bupleurum chinense intercropping (corn/bupleurum chinense), wheat followed by soybean-bupleurum chinense intercropping (wheat-soybean/bupleurum chinense), and sequential planting of bupleurum chinense (wheat-bupleurum chinense). of the three patterns, wheat-soybean/bup ... | 2011 | 21657044 |
| metabonomic study on chronic unpredictable mild stress and intervention effects of xiaoyaosan in rats using gas chromatography coupled with mass spectrometry. | ethnopharmacology: xiaoyaosan (xys), a famous chinese prescription, composed of radix bupleuri (bupleurum chinense dc.), radix angelicae sinensis (angelica sinensis (oliv.) diels), radix paeoniae alba (paeonia lactiflora pall.), rhizoma atractylodis macrocephalae (atractylodes macrocephala koidz.), poria (poria cocos (schw.) wolf), radix glycyrrhizae (glycyrrhiza uralensis fisch.), herba menthae (mentha haplocalyx briq.), and rhizoma zingiberis recens (zingiber officinale rosc.), has been widely ... | 2011 | 21718771 |
| species classification and quality assessment of chaihu (radix bupleuri) based on high-performance liquid chromatographic fingerprint and combined chemometrics methods. | a high-performance liquid chromatographic (hplc) method was established to analyze 36 chaihu (radix bupleuri) samples collected from three species (bupleurum chinense dc., b. scorzonerifolium willd. and b. smithii wolff.). addition of trifluoroacetic acid into the mobile phase resulted in fingerprint chromatograms with stable baselines. there were thirty-two characteristic peaks in the standard fingerprint of b. chinense dc. different recognition pattern methods, including similarity analysis (s ... | 2011 | 21725817 |
| bupleurum chinense dc polysaccharides attenuates lipopolysaccharide-induced acute lung injury in mice. | bupleurum chinense dc had hepato-protective, anti-inflammatory, antipyretic, analgesic, and immunomodulatory effect in traditional chinese medicine. this study was to determine whether the crude polysaccharides isolated from the roots of bupleurum chinense dc (bcps) attenuated lipopolysaccharide (lps)-induced acute lung injury in mice. mice were challenged with lps intratracheally 2h before bcps (20, 40 and 80 mg/kg) administration. the bronchoalveolar lavage fluid (balf) was collected 24h after ... | 2012 | 22112722 |
| Analysis of saikosaponins in rat plasma by anionic adducts-based liquid chromatography tandem mass spectrometry method. | Saikosaponins (SSs) are a class of triterpene saponins with a wide spectrum of bioactivities. A sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed for simultaneous determination of saikosaponin a, saikosaponin c, saikosaponin d and saikosaponin b(2) in rat plasma. Plasma samples were prepared by liquid-liquid extraction. The analytes and the internal standard (IS) digoxin were well separated on an octadecyl column using gradient elution and analyzed by moni ... | 2011 | 21993856 |
| transcriptome analysis of bupleurum chinense focusing on genes involved in the biosynthesis of saikosaponins. | bupleurum chinense dc. is a widely used traditional chinese medicinal plant. saikosaponins are the major bioactive constituents of b. chinense, but relatively little is known about saikosaponin biosynthesis. the 454 pyrosequencing technology provides a promising opportunity for finding novel genes that participate in plant metabolism. consequently, this technology may help to identify the candidate genes involved in the saikosaponin biosynthetic pathway. | 2011 | 22047182 |
| [Advances of studies on seed biology of Bupleurum chinense]. | Bupleurum chinense is a commonly used Chinese medicinal material, which has been used medicinally in China for over 2000 years, the development of it is of great value. There have been great advances of studies on its embryology and seed biology in recent years. In this paper, we make a review of the growth of its embryo and fruit, the characteristics of dormancy and storage and the germination of its seed. Besides, we summarize the reasons of its low germination rate and the achievements in imp ... | 2011 | 22121817 |
| characterization and quantification of major constituents of xue fu zhu yu by uplc-dad-ms/ms. | xue fu zhu yu (xfzy), a classic recipe in traditional chinese medicine (tcm), has been demonstrated to show protective effects on cardiovascular system. for quality control of xfzy products, qualitative analysis using ultra high performance liquid chromatography with diode-array detector-tandem mass spectrometry (uplc-dad-ms) was undertaken. twenty-eight compounds from xfzy were simultaneously detected; among them, seventeen compounds were unequivocally identified, and another eight compounds we ... | 2012 | 22264846 |
| [cloning and distribution pattern of hmgr gene conserved fragment in alisma orientale]. | to clone and study the distribution pattern of 3-hydroxy-3-methylglutaryl coenzyme a reductase (hmgr) gene conserved fragment in alisma orientale. | 2011 | 22259996 |
| polysaccharides from bupleurum chinense impact the recruitment and migration of neutrophils by blocking fmlp chemoattractant receptor-mediated functions. | although neutrophils play important roles in host defense, sometimes they contribute to inflammation-related tissue injuries. thus, restriction of their recruitment to the inflammatory sites is a promising strategy in the amelioration of inflammatory disease. the previous studies reported the anti-inflammatory effects of bupleurum chinense, but the active ingredients and possible mechanism are still unclear. here, we isolated water-soluble polysaccharides (bcps) from b. chinense, to evaluate its ... | 2012 | 23399130 |
| prescription of chinese herbal medicine in pattern-based traditional chinese medicine treatment for depression: a systematic review. | traditional chinese medicine (tcm) treatments are often prescribed based on individuals' pattern diagnoses. a systematic review of randomized controlled trials in chinese and english literatures on tcm pattern-based treatment for depression has therefore been conducted. a total of 61 studies, 2504 subjects, and 27 tcm patterns were included. due to the large variation of tcm pattern among participants, we only analyzed the top four commonly studied tcm patterns: liver qi depression, liver depres ... | 2015 | 26180532 |
| [expression analyses of bcugt3 and bcugt6, and their in vitro expression in escherichia coli]. | the tissue-specific and meja-induced transcriptional levels of bcugt3 and bcugt6 in bupleurum chinense were analyzed in the present study. the transcriptional levels of bcugt3 in root, leaf, flower and fruit were similar and they all were higher than those in stem. the transcriptional level of bcugt6 was the highest in leaf and the lowest in flower among in all tested tissues. with non-treated adventitious roots as control, bcugt6's transcriptional levels were elevated to nearly 2 folds for 2 h, ... | 2014 | 24761629 |
| [expression analysis of glycosyltransferase bcugt1 from bupleurum chinense dc. and its expression in e. coli and the target protein purification]. | the orf sequence of glycosyltransferase gene bcugt1 cloned from bupleurum chinense dc. was analyzed and its three dimentional structure was predicted. using qrt-pcr method, the expression characteristics of bcugt1 after methyl jasmonate (meja) induction and in different plant tissues were investigated. the results showed that bcugt1 may be involved in saikosaponin biosynthesis in b. chinense. thereafter, the recombinant vectors of bcugt1 were constructed for its expression in e. coli. the target ... | 2013 | 24187847 |
| development of a c3c-based elisa method for the determination of anti-complementary potency of bupleurum polysaccharides. | traditionally, determination of inhibitory potency of complement inhibitors is performed by the hemolytic assay. however, this assay is not applicable to the lectin pathway, thus impeding the understanding of complement inhibitors against the overall function of the complement system. the main objective of our study was to develop a specific enzyme-linked immunosorbent assay (elisa) as an alternative method to assess the anti-complement activity, particularly against the lectin pathway. by using ... | 2015 | 26579461 |
| nutrient and metal analyses of chinese herbal products marketed for veterinary use. | many chinese herbs and herbal mixtures are fed to domestic animals for their reputed medicinal properties. these herbs could contribute to the intake of essential nutrients and toxic metals, but their composition is mostly unknown. the purpose of this study was to measure major nutrient (crude protein, crude fat, carbohydrate, fibre) and mineral (ca, p, mg, k, na, fe, zn, cu, mn, mo, s, al, cd, ni, pb) concentrations in samples of fourteen combination formulas labelled for veterinary use and com ... | 2013 | 22289051 |
| dynamic analysis of the endogenous metabolites in depressed patients treated with tcm formula xiaoyaosan using urinary (1)h nmr-based metabolomics. | xiaoyaosan (xys), one of the best-known traditional chinese medicine prescriptions with a long history of use, is composed of bupleurum chinense dc., paeonia lactiflora pall., poria cocos (schw.) wolf, angelica sinensis (oliv.) diels, zingiber officinale rosc., atractylodes macrocephala koidz., glycyrrhiza uralensis fisch., and mentha haplocalyx briq. for centuries, xys has been widely used in china for the treatment of mental disorders such as depression. however, the complicated mechanism unde ... | 2014 | 25448502 |
| saikosaponin a of bupleurum chinense (chaihu) elevates bone morphogenetic protein 4 (bmp-4) during hepatic stellate cell activation. | saikosaponin a (ssa) is a compound extracted from a chinese herb which has been widely used in treating liver diseases such as liver fibrosis. however, the mechanism of ssa in treatment of liver fibrosis still remain unclear. our previous study demonstrated that bmp4 stimulated the expression of smooth muscle alpha actin (α-sma) in the liver. therefore, the current study investigates the effect of ssa on bmp4 expression during hepatic stellate cell activation in a human hepatic stellate cell lin ... | 2013 | 23969230 |
| anti-tumor and immunomodulatory activities induced by an alkali-extracted polysaccharide bcap-1 from bupleurum chinense via nf-κb signaling pathway. | bupleurum chinense is a well-known traditional chinese medicine. polysaccharides extracted from medical plants possess multiple healthy benefits. in the present study, an alkali-extracted polysaccharide (bcap-1) was isolated from bupleurum chinense, and evaluated its physicochemical features, anti-tumor activities and immunomodulatory effects. bcap-1 was obtained by alkali-extraction, ethanol precipitation, and fractionation by deae-cellulose and sepharose cl-6b columns. bcap-1 markedly inhibite ... | 2017 | 27884671 |
| bupleurum chinense polysaccharide inhibit adhesion of human melanoma cells via blocking β1 integrin function. | adhesive interaction contributes toward tumor metastasis and the transmembrane glycoprotein receptor, integrin has been recognized to mediate the adhesion to extracellular matrix thus upregulating tumor metastasis. in the current study, we evaluated the anti-adhesive mechanisms of a water-soluble polysaccharide (bcp) extracted from bupleurum chinense. bcp inhibited integrin-mediated adhesion of human melanoma a375 cells to fibronectin but had no effects on nonspecific adhesion to poly-l-lysine. ... | 2017 | 27842819 |
| toxic polyacetylenes in the genus bupleurum (apiaceae) - distribution, toxicity, molecular mechanism and analysis. | the genus bupleurum includes approximately 200 species that are widely distributed in the northern hemisphere, eurasia and north africa. certain species of this genus have long been used as antiphlogistic, antipyretic and analgesic agents in traditional folk medicine. as described in the chinese pharmacopoeia, the roots of bupleurum chinense dc. and b. scorzonerifolium willd. are the herbal materials that compose chaihu (radix bupleuri), a well-known tcm herb. | 2016 | 27693772 |
| antioxidant and cytotoxic lignans from the roots of bupleurum chinense. | in the search for biologically active compounds from the roots of bupleurum chinense d c., phytochemical investigation of its ethanol extract led to the isolation and identification of a new 8-o-4' neolignan glucoside, saikolignanoside a (1), along with eight known lignans (2-9). their structures were determined on the basis of ir, uv, hresims, and nmr spectroscopic analyses. the antioxidant and cytotoxic effects of isolated compounds were evaluated in vitro. the isolated compounds (ic50 > 200 μ ... | 2017 | 27649745 |
| vinegar amount in the process affected the components of vinegar-baked radix bupleuri and its hepatoprotective effect. | bupleuri radix (in chinese chaihu), the dried roots of bupleurum chinense dc, is a traditional chinese medicine widely used to treat fever, hepatitis, jaundice, nephritis, dizziness. when baked with vinegar, its effect is more focused on liver related disease. this paper was undertaken to determine the best vinegar amount in the processing and explore its key efficacy components. | 2016 | 27599678 |
| [evaluation for merchandise character and quality of bupleurum chinense root from different habitats]. | to evaluate the merchandise character and quality of bupleurum chinense root from different habitats. | 2015 | 26983230 |
| neuroprotective oleanane triterpenes from the roots of bupleurum chinense. | the discovery of new natural compounds with pharmacological properties is an increasingly important field, and a continuous phytochemical investigation of the roots of bupleurum chinense d.c. has led to the isolation of 17 triterpenoids, including three new oleanane triterpenes (1-3) together with 14 known ones. their structures were determined on the basis of 1d and 2d nmr spectra as well as hr-esi-ms data. the cytotoxicities of all compounds against five selected human cancer cell lines were a ... | 2016 | 26883148 |
| anti-melanoma activity of bupleurum chinense, bupleurum kaoi and nanoparticle formulation of their major bioactive compound saikosaponin-d. | bupleurum chinense is a traditional chinese medicinal herb which has been used to treat various inflammatory and infectious diseases, while bupleurum kaoi is an endemic plant in taiwan. we determined whether b. chinense and b. kaoi and their biologically active saikosaponin compounds possess anti-melanoma activity. in addition, we developed a novel saikosaponin-d nanoparticle system to improve its solubility, and evaluated its antiproliferative effects and molecular mechanisms in melanoma cells. | 2016 | 26748071 |
| [clinical observation of breast hyperplasia treated with auricular point sticking therapy and xiaopijian]. | to observe the efficacy difference in the treatment of breast hyperplasia between the combined therapy of auricular point sticking and xiaopijian and the simple application of xiaopijian. | 2015 | 26571890 |
| antioxidant, anticancer and apoptotic effects of the bupleurum chinense root extract in ho-8910 ovarian cancer cells. | the purpose of this study was to evaluate the anticancer, apoptotic and antioxidant properties of bupleurum chinense (b.c) root extract against human epithelial ovarian cancer cells (ho-8910) in vitro. | 2015 | 26537084 |
| cytotoxic triterpenoid glycosides (saikosaponins) from the roots of bupleurum chinense. | as a part of our ongoing studies on cytotoxic triterpenoid saponins from herbal medicines, phytochemical investigation of the roots of bupleurum chinense dc. afforded four new saikosaponins (1-4), along with 16 known ones (5-20). their structures were established by direct interpretation of their spectral data, mainly hr-esi-ms, 1d nmr and 2d nmr, and by comparison with literature data. among them, compound 20 was isolated from the natural product for the first time. the cytotoxicities of all co ... | 2015 | 26259802 |
| evaluation of the genetic diversity of bupleurum using amplified fragment length polymorphism analysis. | radix bupleuri (chaihu), the dried root of the bupleurum plant, is an important component of traditional chinese medicine. in this study, we examined the genetic diversity of 11 bupleurum strains, originating from 7 provinces in china, using amplified fragment length polymorphism analysis. a total of 274 polymorphic bands were obtained using 6 primer combinations, indicating a high level of polymorphism across all strains. an estimation of the relative relationships among strains revealed geneti ... | 2015 | 25867406 |
| [effect of combined application of zinc, boron and molybdenum on yield and saikosaponin a, saikosaponin d contents of bupleurum chinense]. | this research use "3414" fertilizer effect experiments to handle zinc, boron and molybdenum trace element fertilizer, determined the dry matter accumulation and content of saikosaponion a and d, to investigate the different ratio of zinc, boron and molybdenum on yield and saikosaponin a, saikosaponin d contents of bupleurum chinense. found the suitable ratio of zinc, boron and molybdenum play an active role on dry matter accumulation and distribution, the treatment zn2b2mo3 is the best one to pr ... | 2014 | 25850256 |
| robust pls prediction model for saikosaponin a in bupleurum chinense dc. coupled with granularity-hybrid calibration set. | this study demonstrated particle size effect on the measurement of saikosaponin a in bupleurum chinense dc. by near infrared reflectance (nir) spectroscopy. four types of granularity were prepared including powder samples passed through 40-mesh, 65-mesh, 80-mesh, and 100-mesh sieve. effects of granularity on nir spectra were investigated, which showed to be wavelength dependent. nir intensity was proportional to particle size in the first combination-overtone and combination region. local partia ... | 2015 | 25821634 |
| caffeic acid derivatives from bupleurum chinense. | in this study, caffeic acid (ca) and its three derivatives including 3-caffeoylquinic acid (3-cqa, neochlorogenic acid), 4-caffeoylquinic acid (4-cqa, cryptochlorogenic acid), and 5-caffeoylquinic acid (5-cqa, chlorogenic acid) were identified in bupleurum chinense aerial parts using reverse-phase high-performance liquid chromatography (rp-hplc) with photodiode array (pda) detector, reference compounds and chemical reactions. separation was performed on a c18 column using gradient elution with 4 ... | 2017 | 25657804 |
| [grain filling dynamics and germination characteristics of bupleurum chinense seeds]. | bupleurum chinense used in the study were cultivated in the experimental fields of gansu agricultural university for three years. the seeds of b. chinense were collected every 3 days 10 d after the blossom. the result showed that the 1 000-grain fresh weight reached the maximum 43 d after the blossom and then decreased rapidly, at the mature period the fresh weight of seeds were falling to the same level of the dry weight. the dynamic change of the grain dry matter accumulation showed as an s-sh ... | 2014 | 25612430 |
| [identification of bupleurum chinense and b. longiradiatum based on its2 barcode]. | in this study, its2 barcode was used to identify bupleurum chinense and b. longiradiatum. the its2 regions of 48 samples were amplified and sequenced. the sequences obtained above were aligned and the k2p distances were calculated. we used three methods, blast1, nearest distance and phylogenetic tree (nj-tree), to test the identification ability. the results showed that the maximum intraspecific genetic distance of b. chinense was 0.013, and the minimum interspecific genetic distance between b. ... | 2014 | 25244736 |
| autophagic effects of chaihu (dried roots of bupleurum chinense dc or bupleurum scorzoneraefolium wild). | chaihu, prepared from the dried roots of bupleurum chinense dc (also known as bei chaihu in chinese) or bupleurum scorzoneraefolium wild (also known as nan chaihu in chinese), is a herbal medicine for harmonizing and soothing gan (liver) qi stagnation. substantial pharmacological studies have been conducted on chaihu and its active components (saikosaponins). one of the active components of chaihu, saikosaponin-d, exhibited anticancer effects via autophagy induction. this article reviews the pha ... | 2014 | 25228909 |
| comparison of root transcriptomes and expressions of genes involved in main medicinal secondary metabolites from bupleurum chinense and bupleurum scorzonerifolium, the two chinese official radix bupleuri source species. | radix bupleuri, roots of bupleurum species, is a widely used traditional chinese medicine. here, we compared the root transcriptomes of both bupleurum chinense dc. and bupleurum scorzonerifolium willd. a total of 313 483 and 342 263 high quality expressed sequence tags were obtained, respectively. in addition, 17 117 (59.2%) and 19 416 (62.8%) unigenes for b. chinense and b. scorzonerifolium had homologous genes in the opposite dataset. for b. chinense, kyoto encyclopedia of genes and genomes da ... | 2015 | 25117935 |