| identification of proteases from periodontopathogenic bacteria as activators of latent human neutrophil and fibroblast-type interstitial collagenases. | activation of latent human fibroblast-type and neutrophil interstitial procollagenases as well as degradation of native type i collagen by supra- and subgingival dental plaque extracts, an 80-kda trypsinlike protease from porphyromas gingivalis (atcc 33277), a 95-kda chymotrypsinlike protease from treponema denticola (atcc 29522), and selected bacterial species commonly isolated in periodontitis was studied. the bacteria included were prevotella intermedia (atcc 25261), prevotella buccae (es 57) ... | 1992 | 1398963 |
| bacteroides spp. in dental root canal infections. | a summary of a series of bacteriological studies of endodontic infections is presented in this article. the bacteriology of 62 root canal infections was studied with special attention focused on the occurrence, role and taxonomy of bacteroides spp. all infections except one were mixed infections dominated usually by anaerobic bacteria. four to 6 different species were present in most canals. species of the genus bacteroides were found more frequently than species of any other genus. seventy-eigh ... | 1989 | 2598878 |
| mitsuokella dentalis in human periodontitis. | the occurrence of mitsuokella dentalis in periodontitis was determined by culture and dna probe detection. subgingival paper-point samples from 480 periodontitis patients were transported in vmga iii, plated onto brucella agar with 5% sheep blood and incubated anaerobically for 7 days. presumptive identification was based on a colony morphology resembling a water drop and biochemical characteristics. dna probe detection was performed on paper-point samples using a digoxigenin-labeled cellular m. ... | 1994 | 7478766 |
| the tet(q) gene in bacteria isolated from patients with refractory periodontal disease. | twenty-two tetracycline-resistant (tetr) anaerobic and facultative anaerobic bacteria isolated from periodontal pockets of 12 patients with refractory periodontitis were examined for the presence of the tet q determinant by dna-dna hybridization. dot blots of bacterial dna were tested with an intragenic digoxigenin-labelled tet(q) probe consisting of a 1.45 kb ecori/pvuii fragment from plasmid pnfd13-2. southern blots of chromosomal dna digested with the restriction enzyme ecori were also examin ... | 1994 | 7478767 |
| 16s rrna gene similarities indicate that hallella seregens (moore and moore) and mitsuokella dentalis (haapsalo et al.) are genealogically highly related and are members of the genus prevotella: emended description of the genus prevotella (shah and collins) and description of prevotella dentalis comb. nov. | because of similarities in the cellular fatty acid compositions of hallella seregens and mitsuokella dentalis, we determined the 16s rrna gene sequences of the type strains of these species to assess their relationship. a very high level of sequence relatedness (approximately 99.8%) was found between h. seregens and m. dentalis, indicating that these species are genealogically closely related. a comparative sequence analysis revealed that these two species are members of the genus prevotella and ... | 1995 | 7547307 |
| recently described clinically important anaerobic bacteria: taxonomic aspects and update. | a new method of identifying bacteria, phylogenetic 16s rrna sequencing, has led to major reorganizations among most genera of anaerobic bacteria. the pigmented prevotella species now comprise seven species including p. nigrescens and p. tannerae; p. intermedia/p. nigrescens-like organisms await inclusion. the former mitsuokella dentalis and hallella seregens were transferred to prevotella as one species, p. dentalis. p. enoeca is a new nonpigmenting prevotella. the genus porphyromonas currently ... | 1997 | 9310640 |
| prevotella multisaccharivorax sp. nov., isolated from human subgingival plaque. | six bacterial strains isolated from the human oral cavity, pppa16, pppa20(t), pppa24, pppa31, eppa6 and eppa7, were characterized by determining phenotypic and biochemical features, cellular fatty acid profiles, menaquinone profiles and phylogenetic position based on 16s rrna gene sequence analysis. 16s rrna gene sequence analysis showed that the isolates represented the same species of the genus prevotella. the strains were related to prevotella dentalis with about 89% similarity. in addition, ... | 2005 | 16166675 |
| prevotella bergensis sp. nov., isolated from human infections. | eight strains of anaerobic gram-negative bacilli isolated from infections of the skin and soft tissues were subjected to a comprehensive range of phenotypic and genotypic tests. 16s rrna gene sequence analysis revealed the strains to constitute a homogeneous group, distinct from species with validly published names but related to a cluster including prevotella buccae, prevotella dentalis and prevotella baroniae. a novel species, prevotella bergensis sp. nov., is proposed to accommodate these str ... | 2006 | 16514036 |
| genome-based taxonomic classification of bacteroidetes. | the bacterial phylum bacteroidetes, characterized by a distinct gliding motility, occurs in a broad variety of ecosystems, habitats, life styles, and physiologies. accordingly, taxonomic classification of the phylum, based on a limited number of features, proved difficult and controversial in the past, for example, when decisions were based on unresolved phylogenetic trees of the 16s rrna gene sequence. here we use a large collection of type-strain genomes from bacteroidetes and closely related ... | 2016 | 28066339 |
| discovery of β-1,4-d-mannosyl-n-acetyl-d-glucosamine phosphorylase involved in the metabolism of n-glycans. | a gene cluster involved in n-glycan metabolism was identified in the genome of bacteroides thetaiotaomicron vpi-5482. this gene cluster encodes a major facilitator superfamily transporter, a starch utilization system-like transporter consisting of a tonb-dependent oligosaccharide transporter and an outer membrane lipoprotein, four glycoside hydrolases (α-mannosidase, β-n-acetylhexosaminidase, exo-α-sialidase, and endo-β-n-acetylglucosaminidase), and a phosphorylase (bt1033) with unknown function ... | 2013 | 23943617 |