| intrageneric relationships of enterococci as determined by reverse transcriptase sequencing of small-subunit rrna. | the 16s ribosomal ribonucleic acid (rrna) sequences of eleven enterococcus species were determined by reverse transcription in an attempt to clarify their intrageneric relationships. comparative analysis of the sequence data revealed the presence of several species groups within the genus. the species e. avium, e. malodoratus, e. pseudoavium and e. raffinosus formed a distinct group as did e. durans, e. faecium, e. hirae and e. mundtii and the pair of species e. casseliflavus and e. gallinarum. ... | 1991 | 1712504 |
| the phylogeny of aerococcus and pediococcus as determined by 16s rrna sequence analysis: description of tetragenococcus gen. nov. | the phylogenetic interrelationships of the genera pediococcus and aerococcus were investigated using reverse transcriptase sequencing of 16s rrna. the genus pediococcus was found to be phylogenetically heterogeneous. the four species p. acidilactici, p. damnosus, p. parvulus and p. pentosaceus formed a phylogenetically distinct group. within this pediococcal cluster, p. acidilactici was closely related to p. pentosaceus whereas p. damnosus showed a specific relationship with p. parvulus. the spe ... | 1990 | 2227360 |
| pucl287 plasmid from tetragenococcus halophila (pediococcus halophilus) atcc 33315 represents a new theta-type replicon family of lactic acid bacteria. | a cryptic plasmid, pucl287, was isolated from tetragenococcus halophila (pediococcus halophilus) atcc 33315. it had a theta-type mechanism of replication in its natural host. its minimal replicon, rep287, was isolated on a 1.6-kb ecori fragment. the rep287 host range included the genera pediococcus, enterococcus, lactobacillus and leuconostoc but not genus lactococcus. plasmids hybridizing to pucl287 are rare among lactic acid bacteria. as assessed by hybridization, rep287 is dissimilar to pam b ... | 1995 | 7750734 |
| characterization and replication mode determination of the minimal replicon of tetragenococcus halophila atcc33315 plasmid pucl287. | pucl287 is a cryptic plasmid of tetragenococcus halophila (formerly pediococcus halophilus) atcc33315 of relatively small size (8.7 kb). its minimal replicon was located on a 1235 bp mami-ecori fragment. this minimal replicon contains a non-translated region, followed by a gene encoding a putative 311 amino acid protein. deletion experiments showed that the non-translated region corresponds to the replication origin. determination of the replication mode was carried out in enterococcus faecalis ... | 1995 | 8824766 |
| lactic acid bacteria of foods and their current taxonomy. | application of molecular genetic techniques to determine the relatedness of food-associated lactic acid bacteria has resulted in significant changes in their taxonomic classification. during the 1980s the genus streptococcus was separated into the three genera enterococcus, lactococcus and streptococcus. the lactic acid bacteria associated with foods now include species of the genera carnobacterium, enterococcus, lactobacillus, lactococcus, leuconostoc, oenococcus, pediococcus, streptococcus, te ... | 1997 | 9168311 |
| tetragenococcus muriaticus sp. nov., a new moderately halophilic lactic acid bacterium isolated from fermented squid liver sauce. | a total of 11 strains of moderately halophilic histamine-producing bacteria isolated from fermented squid liver sauce were studied phenotypically, genotypically, and phylogenetically. these strains are considered members of the genus tetragenococcus based on their physiological, morphological, and chemotaxonomic characteristics. a 16s rrna gene sequence analysis showed that these strains clustered with, but were separate from, tetragenococcus halophilus. the results of dna-dna hybridization expe ... | 1997 | 9226914 |
| molecular analysis of the replication region of the theta-replicating plasmid pucl287 from tetragenococcus (pediococcus) halophilus atcc33315. | the complete nucleotide sequence of the 8.7-kb theta-replicating plasmid pucl287 from tetragenococcus halophilus (formerly pediococcus halophilus) atcc33315 has been determined. the replication region was identified and analyzed. its nucleotide sequence contains an untranslated region, the replication origin, followed by two open reading frames (orfs) encoding two proteins of 311 (repa287) and 168 (repb287) amino acids, respectively. evidence is presented to show that repa287 represents the plas ... | 1997 | 9294035 |
| growth, maintenance and fermentation pattern of the salt-tolerant lactic acid bacterium tetragenococcus halophila in anaerobic glucose limited retention cultures. | the homofermentative lactic acid bacterium tetragenococcus halophila showed mixed acid fermentation at low growth-rates under glucose limiting conditions and in the presence of 10% nacl. maximum growth yields in fermentors with cell retention were not affected by ph, but maintenance requirement was at ph 5.2 four times higher than at ph 7.0. despite the high salt-concentration of the medium, maintenance requirements were low compared to other lactic acid bacteria. the possible causes of the obse ... | 1997 | 9403109 |
| sequencing and characterization of the xyl operon of a gram-positive bacterium, tetragenococcus halophila. | the xyl operon of a gram-positive bacterium, tetragenococcus halophila (previously called pediococcus halophilus), was cloned and sequenced. the dna was about 7.7 kb long and contained genes for a ribose binding protein and part of a ribose transporter, xylr (a putative regulatory gene), and the xyl operon, along with its regulatory region and transcription termination signal, in this order. the dna was at rich, the gc content being 35.8%, consistent with the gc content of this gram-positive bac ... | 1998 | 9647823 |
| xylose transport insensitivity to catabolite inhibition by phosphoenolpyruvate:sugar phosphotransferase system in tetragenococcus halophila. | tetragenococcus halophila accumulates glucose and 2-deoxyglucose (dglc) via the phosphoenolpyruvate:sugar phosphotransferase system (pts), and pentoses, maltose, and glycerol via non-pts carriers. based on the discovery that xylose metabolism in t. halophila was subject to catabolite repression but not to catabolite inhibition, we designed a selection protocol for thermosensitive mutants pleiotropically unable to use sugars. one such mutant was a ptsi mutant with a thermosensitive enzyme i (ei) ... | 1998 | 9805368 |
| molecular cloning and functional expression in lactobacillus plantarum 80 of xylt, encoding the d-xylose-h+ symporter of lactobacillus brevis. | a 3-kb region, located downstream of the lactobacillus brevis xyla gene (encoding d-xylose isomerase), was cloned in escherichia coli tg1. the sequence revealed two open reading frames which could code for the d-xylulose kinase gene (xylb) and another gene (xylt) encoding a protein of 457 amino acids with significant similarity to the d-xylose-h+ symporters of e. coli, xyle (57%), and bacillus megaterium, xylt (58%), to the d-xylose-na+ symporter of tetragenococcus halophila, xyle (57%), and to ... | 1998 | 9835554 |
| selective fermentation of xylose by a mutant of tetragenococcus halophila defective in phosphoenolpyruvate:mannose phosphotransferase, phosphofructokinase, and glucokinase. | tetragenococcus halophila is a gram-positive halophilic lactic acid bacterium used for soy sauce fermentation. we isolated a mutant, t. halophila 3e4, triply defective in phosphoenolpyruvate:mannose phosphotransferase, phosphofructokinase, and glucokinase. 3e4 selectively metabolized pentoses such as xylose and arabinose in the presence of hexoses such as glucose and galactose. we present here an example of the metabolic engineering of catabolite control. | 1998 | 9836445 |
| glycine betaine, carnitine, and choline enhance salinity tolerance and prevent the accumulation of sodium to a level inhibiting growth of tetragenococcus halophila. | natural-abundance (13)c-nuclear magnetic resonance was used to probe the intracellular organic solute content of the moderately halophilic bacterium tetragenococcus halophila. when grown in complex growth media supplemented or not with nacl, t. halophila accumulates glycine betaine and carnitine. unlike other moderate halophiles, t. halophila was not able to produce potent osmoprotectants (such as ectoines and glycine betaine) through de novo synthesis when cultured in defined medium under hyper ... | 2000 | 10653711 |
| differentiation of tetragenococcus populations occurring in products and manufacturing processes of puffer fish ovaries fermented with rice-bran. | tetragenococcus strains isolated from the manufacturing process of japanese puffer fish ovaries fermented with rice-bran were characterized and differentiated phenotypically and genotypically. a total of 413 tetragenococcus isolates were evaluated. on the basis of five representative substrates, the isolates were grouped into seven groups. an rflp (restriction fragment length polymorphisms) analysis of the 16s rrna gene of representative strains of major groups revealed that they could be groupe ... | 2000 | 10857547 |
| ps86, a new theta-replicating plasmid from enterococcus faecalis. | the complete nucleotide sequence of the small (5149 bp) and cryptic plasmid ps86 from enterococcus faecalis ssp. faecalis s-86 has been determined. sequence analysis revealed six putative open reading frames (orfs) encoding polypeptides of 28.3, 11.5, 8.4, 65.1, 7.3, and 11.96 kda each. based on sequence similarity, two cassettes have been identified in ps86: orf1 codes for the replication initiation protein (rep); orf4 codes for a putative mobilization protein that shows similarities to mob/pre ... | 2000 | 10977892 |
| a rapid pcr based method to distinguish between lactococcus and enterococcus. | phenotypic characterisation of lactococcus and enterococcus species remains unreliable as strains of both genera have been isolated which do not conform to the traditional criteria for separation of these genera. a bank of 131 isolates was phenotypically characterised by three methods: (a) traditional broth tests, (b) api rapid id 32 strep and (c) bbl crystal id kits. differences in genus designation between commercial kits were evident for 12 strains (9%), while 7 strains (5%) remained unidenti ... | 2000 | 11249021 |
| a nested array of rrna targeted probes for the detection and identification of enterococci by reverse hybridization. | complete 23s and almost complete 16s rrna gene sequences were determined for the type strains of the validly described enterococcus species, melissococcus pluton and tetragenococcus halophilus. a comprehensive set of rrna targeted specific oligonucleotide hybridization probes was designed according to the multiple probe concept. in silico probe design and evaluation was performed using the respective tools of the arb program package in combination with the arb databases comprising the currently ... | 2000 | 11249027 |
| the replicon of the cryptic plasmid psbo1 isolated from streptococcus bovis jb1. | the cryptic plasmid psbo1 (3904 bp) was isolated from streptococcus bovis jb1. psbo1 contained an open reading frame (orf) that is homologous to sequences encoding the replication protein (rep) in pefc1 (isolated from enterococcus faecalis), psk639 (staphylococcus epidermidis), pla103 (lactobacillus acidophilus), and pucl287 (tetragenococcus halophila). in addition, four 22-bp direct repeats (drs) were located upstream of the putative replication gene (rep) of psbo1. recombinant plasmids (psbe10 ... | 2001 | 11375657 |
| histamine formation by tetragenococcus muriaticus, a halophilic lactic acid bacterium isolated from fish sauce. | we examined histamine formation in cultures of tetragenococcus muriaticus, a halophilic lactic acid bacterium isolated from fish sauce. t. muriaticus formed histamine in low acidity (ph 5.8), o2 limiting conditions with optimal nacl and glucose concentrations of 5-7% (w/v) and above 1%, respectively. histamine formation could not be prevented even at 20% (w/v) nacl, indicating that nacl could not prevent histamine formation by this bacterium. a conspicuous amount of histamine accumulated only du ... | 2001 | 11759764 |
| widespread distribution of the bacteriocin-producing lactic acid cocci in miso-paste products. | a survey was conducted on the ecological distribution of bacteriocin-producing lactic acid bacteria in miso-pastes, a typical fermented food in japan. methods and results nine miso-pastes were sampled for isolation of bacteriocin-producers. almost all isolated enterococcal strains produced bacteriocins but no isolated tetragenococci did so. the bacteriocin-producing isolates were divided into nine groups by phenotypic tests. as the phenotypic characters were highly diverse, these strains could n ... | 2002 | 11966910 |
| plasmid-encoded asp operon confers a proton motive metabolic cycle catalyzed by an aspartate-alanine exchange reaction. | tetragenococcus halophila d10 catalyzes the decarboxylation of l-aspartate with nearly stoichiometric release of l-alanine and co(2). this trait is encoded on a 25-kb plasmid, pd1. we found in this plasmid a putative asp operon consisting of two genes, which we designated aspd and aspt, encoding an l-aspartate-beta-decarboxylase (aspd) and an aspartate-alanine antiporter (aspt), respectively, and determined the nucleotide sequences. the sequence analysis revealed that the genes of the asp operon ... | 2002 | 12003930 |
| purification and properties of a histidine decarboxylase from tetragenococcus muriaticus, a halophilic lactic acid bacterium. | a histidine decarboxylase from tetragenococcus muriaticus, a halophilic histamine-producing bacterium isolated from japanese fermented squid liver sauce, was purified to homogeneity, for the first time. | 2002 | 12010554 |
| the groesl operon of the halophilic lactic acid bacterium tetragenococcus halophila. | the groesl operon of the halophilic lactic acid bacterium tetragenococcus halophila was cloned by a pcr-based method. the molecular masses of groes and groel proteins were calculated to be 10,153 and 56,893 da, respectively. the amount of groesl mrna was increased 3.8-fold by heat shock (45 degrees c), and 4-fold by high nacl (3-4 m). the bacillus subtilis sigmaa-like constitutive promoter existed in front of groes, and was used under both normal and stress (heat shock and high salinity) conditi ... | 2002 | 12092841 |
| lactic acid bacteria isolated from soy sauce mash in thailand. | fourteen sphere-shaped and 30 rod-shaped lactic acid bacteria were isolated from soy sauce mash of two factories in thailand. these strains were separated into two groups, group a and group b, by cell shape and dna-dna similarity. group a contained 14 tetrad-forming strains, and these strains were identified as tetragenococcus halophilus by dna similarity. group b contained 30 rod-shaped bacteria, and they were further divided into four subgroups, b1, b2, b3, and b4, and three ungrouped strains ... | 2002 | 12469319 |
| potential osmoprotectants for the lactic acid bacteria pediococcus pentosaceus and tetragenococcus halophila. | the physiological responses of the lactic acid bacteria pediococcus pentosaceus and tetragenococcus halophila (formely known as p. halophila), subjected to osmotic stress in the presence of molecules known to act as osmoprotectants for other bacteria were studied. in a defined medium, glycine betaine, dimethylsulfonioacetate, choline, proline and l-carnitine were able to relieve inhibition of growth at 0.8 m nacl. the five compounds were shown to efficiently compete with glycine betaine transpor ... | 2003 | 12781949 |
| taxonomic homogeneity of a salt-tolerant lactic acid bacteria isolated from shoyu mash. | forty-seven salt-tolerant lactic acid bacteria, which had been isolated from different places and grown in 15% nacl, were examined to assess their taxonomic heterogeneity. among the isolates, 42 were isolated from shoyu mash during the acid fermentation phase, 2 were from miso and 3 were from anchovy pickles. all isolates were identified as tetragenococcus halophilus on the basis of dna relatedness values. we further examined 102 phenotypic characteristics of them. the isolates exhibited differe ... | 2003 | 12833212 |
| isolation and characterization of buta, a secondary glycine betaine transport system operating in tetragenococcus halophila. | through functional complementation of an escherichia coli mutant defective in glycine betaine uptake, we identified a single-component glycine betaine transporter from tetragenococcus halophila, a moderate halophilic lactic acid bacterium. dna sequence analysis characterized the buta protein as a member of the betaine choline carnitine transporter (bcct) family, that includes a variety of previously characterized compatible solute transporters such as opud from bacillus subtilis, ectp and betp f ... | 2003 | 14629018 |
| isolation and characterization of halophilic lactic acid bacteria isolated from "terasi" shrimp paste: a traditional fermented seafood product in indonesia. | lactic acid bacteria from "terasi" shrimp paste, a highly popular fermented seafood in indonesia were isolated and characterized. viable cell counts were 10(4) to 10(6) cfu/g on mrs medium. all the isolates were catalase-negative, gram-positive cocci and were able to grow at 15% nacl. numerical phenotypic analysis showed that the isolates clustered into one group. however, they could be classified into two types: the tetragenococcus halophilus group and the t. muriaticus group as revealed by a r ... | 2003 | 14673751 |
| effect of culture conditions on lactic acid production of tetragenococcus species. | to investigate the effects of the salt concentration, incubation temperature and initial ph of the medium on the fermentative ability of the halophilic lactic acid bacteria, tetragenococcus muriaticus and t. halophilus. | 2004 | 15139912 |
| membrane topology of the electrogenic aspartate-alanine antiporter aspt of tetragenococcus halophilus. | aspt is an electrogenic aspartate:alanine exchange protein that represents the vectorial component of a proton-motive metabolic cycle found in some strains of tetragenococcus halophilus. aspt is the sole member of a new family, the aspartate: alanine exchanger (aae) family, in secondary transporters, according to the computational classification proposed by saier et al. (http://www.biology.ucsd.edu/~msaier/transport/). we analyzed the topology of aspt biochemically, by using fusion methods in co ... | 2005 | 15670744 |
| biochemical and genetic evidence for the transfer of enterococcus solitarius collins et al. 1989 to the genus tetragenococcus as tetragenococcus solitarius comb. nov. | phylogenetic analysis of 16s rrna gene sequences revealed that enterococcus solitarius is not a member of the genus enterococcus, but is related to species of the genus tetragenococcus. on a phylogenetic tree, e. solitarius clustered with tetragenococcus halophilus and tetragenococcus muriaticus, with which it showed the highest 16s rrna gene sequence similarity level (about 94 %). phenotypic studies indicated that e. solitarius was also unable to produce acid from lactose, providing further evi ... | 2005 | 15774629 |
| tetragenococcus koreensis sp. nov., a novel rhamnolipid-producing bacterium. | a gram-positive, non-motile, non-spore-forming coccus (strain js(t)) was isolated from kimchi (a traditional korean food) and investigated using a polyphasic taxonomic approach. the 16s rrna gene sequence similarity between strain js(t) and its closest relative, tetragenococcus halophilus iam 1676(t), was 98.1%. the level of dna-dna relatedness between the two strains was 9.7%. strain js(t) had a dna g+c content of 38.3% and a cellular fatty acid profile containing 16:0, 18:1 and cyclo fatty aci ... | 2005 | 16014460 |
| molecular characterization and regulatory analysis of dnak operon of halophilic lactic acid bacterium tetragenococcus halophila. | we have cloned and characterized the dnak operon of tetragenococcus halophila jcm5888. nucleotide sequence analysis of cloned fragments showed that the dnak operon consists of four open reading frames with the organization hrca-grpe-dnak-dnaj. two regulatory circe (controlling inverted repeat of chaperone expression) elements were identified in the region up-stream of hrca. the t. halophila dnak encoded a protein of 618 amino acids with a calculated molecular mass of 67 kda. the deduced amino ac ... | 2002 | 16233219 |
| effect of heterologous expression of molecular chaperone dnak from tetragenococcus halophilus on salinity adaptation of escherichia coli. | molecular chaperone dnak of halophilic tetragenococcus halophilus jcm5888 was characterized under salinity conditions both in vitro and in vivo. the dnak gene was cloned into an expression vector and transformed into escherichia coli. the dnak protein obtained from the recombinant e. coli showed a significantly higher refolding activity of denatured lactate dehydrogenase than that from non-halophilic lactococcus lactis under nacl concentrations higher than 1 m. e. coli without the overexpression ... | 2003 | 16233497 |
| reconstitution and function of tetragenococcus halophila chaperonin 60 tetradecamer. | tetragenococcus halophila originally isolated from soy sauce is a halophilic lactic acid bacterium which can grow under 4 m sodium chloride. t. halophila chaperonin composed of a core moiety of chaperonin 60 (cpn60) and a lid moiety of chaperonin 10 (cpn10), is thought to contribute to host halotolerant capability. in this report, we reconstituted and characterized the core complex of t. halophila chaperonin by using a recombinant t. halophila cpn60 (tcpn60) overexpressed in escherichia coli. th ... | 2005 | 16233750 |
| monitoring the lactic acid bacterial diversity during shochu fermentation by pcr-denaturing gradient gel electrophoresis. | the presence of lactic acid bacteria (lab) during shochu fermentation was monitored by pcr-denaturing gradient gel electrophoresis (dgge) and by bacteriological culturing. no lab were detected from fermented mashes by pcr-dgge using a universal bacterial pcr primer set. however, pcr-dgge using a new primer specific for the 16s rdna of lactococcus, streptococcus, tetragenococcus, enterococcus, and vagococcus and two primers specific for the 16s rdna of lactobacillus, pediococcus, leuconostoc, and ... | 2005 | 16233780 |
| catellicoccus marimammalium gen. nov., sp. nov., a novel gram-positive, catalase-negative, coccus-shaped bacterium from porpoise and grey seal. | two strains of an unknown gram-positive, catalase-negative, facultatively anaerobic, coccus-shaped bacterium, originating from a porpoise and a grey seal, were characterized using phenotypic, biochemical and molecular phylogenetic methods. chemical studies revealed the presence of a cell-wall murein based on l-lysine (type l-lys-gly-d-asp) and a dna g+c content of 38 mol%. comparative 16s rrna gene sequencing showed that this unidentified coccus-shaped organism formed a hitherto unknown subline ... | 2006 | 16449452 |
| characterization of tetragenococcus halophila populations in indonesian soy mash (kecap) fermentation. | chinese- and japanese-type fermented soy sauces are made of different plant materials. the lactic acid bacterium tetragenococcus halophila is present and grows in both types. on the basis of the difference in sugar composition and content in the plant materials, differences in the populations of t. halophila bacteria were expected. however, obvious differences were found only regarding the utilization of l-arabinose. in the chinese type, almost all isolates utilized l-arabinose, while in the jap ... | 1996 | 16535286 |
| biodiversity of moderately halophilic bacteria in hypersaline habitats in egypt. | screening bacteria from different saline environments in alexandria. egypt, lead to the isolation of 76 gram-negative and 14 gram-positive moderately halophilic bacteria. the isolates were characterized taxonomically for a total of 155 features. these results were analyzed by numerical techniques using simple matching coefficient (ssm) and the clustering was achieved by the unweighed pair-group method of association (upgma). at 75% similarity level the gram-negative bacteria were clustered in 7 ... | 2006 | 16778349 |
| isolation and characterization of lactic acid bacteria from suan-tsai (fermented mustard), a traditional fermented food in taiwan. | to isolate, characterize, and identify lactic acid bacteria (lab) in suan-tsai (fermented mustard), a traditional fermented food in taiwan. | 2006 | 16834599 |
| isolation and characterization of lactic acid bacteria from dochi (fermented black beans), a traditional fermented food in taiwan. | to isolate, characterize and identify lactic acid bacteria (lab) in dochi (fermented black beans), a traditional fermented food in taiwan. | 2006 | 16869910 |
| structural and functional conversion of molecular chaperone clpb from the gram-positive halophilic lactic acid bacterium tetragenococcus halophilus mediated by atp and stress. | in this study, we report the purification, initial structural characterization, and functional analysis of the molecular chaperone clpb from the gram-positive, halophilic lactic acid bacterium tetragenococcus halophilus. a recombinant t. halophilus clpb (clpb(tha)) was overexpressed in escherichia coli and purified by affinity chromatography, hydroxyapatite chromatography, and gel filtration chromatography. as demonstrated by gel filtration chromatography, chemical cross-linking with glutaraldeh ... | 2006 | 16997952 |
| membrane topology of aspartate:alanine antiporter aspt from comamonas testosteroni. | we cloned the aspt gene encoding the l-aspartate:l-alanine antiporter asptct in comamonas testosteroni genomic dna. analysis of the nucleotide sequence revealed that c. testosteroni has an asp operon containing aspt upstream of the l-aspartate 4-decarboxylase gene, and that the gene order of the asp operon of c. testosteroni is the inverse of that of tetragenococcus halophilus. we used proteoliposomes to confirm the transport processes of asptct. to elucidate the two-dimensional structure of asp ... | 2007 | 17158863 |
| topology of aspt, the aspartate:alanine antiporter of tetragenococcus halophilus, determined by site-directed fluorescence labeling. | the gram-positive lactic acid bacterium tetragenococcus halophilus catalyzes the decarboxylation of l-aspartate (asp) with release of l-alanine (ala) and co(2). the decarboxylation reaction consists of two steps: electrogenic exchange of asp for ala catalyzed by an aspartate:alanine antiporter (aspt) and intracellular decarboxylation of the transported asp catalyzed by an l-aspartate-beta-decarboxylase (aspd). aspt belongs to the newly classified aspartate:alanine exchanger family (transporter c ... | 2007 | 17660287 |
| protective effect of hop beta-acids on microbial degradation of thick juice during storage. | this study assessed the value of a commercial alkaline solution of hop beta-acids (hba) for prevention of microbial degradation of thick juice, a concentrated intermediate product in the production of beet sugar. | 2008 | 17850321 |
| immunomodulatory effect of halophilic lactic acid bacterium tetragenococcus halophilus th221 from soy sauce moromi grown in high-salt medium. | a halophilic lactic acid bacterium, tetragenococcus halophilus, was found to possess an immunomodulatory activity that promotes t helper type 1 (th1) immunity in addition to its important roles in soy sauce brewing. strain th221 was selected from 151 strains isolated from soy sauce (shoyu) moromi, since it induced strong interleukin (il)-12 production by mouse peritoneal macrophages in vitro. the relationship between the salt concentration in the medium and the il-12 production-inducing activity ... | 2008 | 18061297 |
| predominance of tetragenococcus halophilus as the cause of sugar thick juice degradation. | the industrial storage of sugar thick juice was simulated on a laboratory scale. terminal restriction fragment length polymorphism (t-rflp) analysis and the application of clone libraries in parallel with classical microbiology were used to study the bacterial diversity and all revealed a dominance (>99%) of tetragenococcus halophilus during storage. the degradation of thick juice correlated with the appearance of l-lactic acid and high concentrations of t. halophilus. in addition, pure cultures ... | 2008 | 18206785 |
| changes in bacterial communities accompanied by aggregation in a fed-batch composting reactor. | the contents of fed-batch composting (fbc) reactors often aggregate after prolonged operation. this process leads to irreversible breakdown of the decomposition reaction and possible alteration of the bacterial communities. we compared the structures of bacterial communities in reactors under aggregate and optimal conditions. the results of 16s rrna gene clone analysis showed that populations of the family bacillaceae (such as bacillus spp., cerasibacillus spp., gracilibacillus spp.), which domi ... | 2008 | 18231830 |
| in vivo and in vitro complementation study comparing the function of dnak chaperone systems from halophilic lactic acid bacterium tetragenococcus halophilus and escherichia coli. | in this study, we characterized the dnak chaperone system from tetragenococcus halophilus, a halophilic lactic acid bacterium. an in vivo complementation test showed that under heat stress conditions, t. halophilus dnak did not rescue the growth of the escherichia coli dnak deletion mutant, whereas t. halophilus dnaj and grpe complemented the corresponding mutations of e. coli. purified t. halophilus dnak showed intrinsic weak atpase activity and holding chaperone activity in vitro, but t. halop ... | 2008 | 18323638 |
| characterization of the first oral vagococcus isolate from a root-filled tooth with periradicular lesions. | strain endo-eh was isolated from a root-filled tooth associated with periradicular lesions. after subculturing on columbia blood agar, phenotypic and genomic characterizations using different biochemical test systems, automated ribotyping, maldi-tof mass spectronomy, antibiotic susceptibility testing, and 16s rrna gene sequence analysis were applied for further analysis. phenotypic characterization identified this strain as vagococcus fluvialis. riboprint pattern analysis and 16s rrna sequencing ... | 2008 | 18560938 |
| analysis of a 30 kbp plasmid encoding histidine decarboxylase gene in tetragenococcus halophilus isolated from fish sauce. | in order to analyze the genes related to the histamine production, a strain of histamine producing halophilic bacteria, referred to as strain h, was isolated using enrichment culture and dilution-to-extinction methods with histidine broth inoculated from the fish sauce mashes. the two japanese fish sauce mashes used, accumulate over 1000 mg/l of histamine. phenotypic and 16 s rrna gene sequence analyses identified strain h as tetragenococcus halophilus, the predominant histamine producing bacter ... | 2008 | 18573560 |
| present knowledge of the bacterial microflora in the extreme environment of sugar thick juice. | the diversity of the bacterial population in sugar thick juice, an intermediate product in the production of beet sugar, which exhibits an extreme, osmophilic environment with a water activity value (a(w)) less than 0.86, was assessed with both culture-dependent and -independent 16s ribosomal rna (rrna) gene-based analyses. in comparison with previous studies, the number of different thick juice bacterial species increased from 29 to 72. remarkably, a limited, gram-positive, culturable flora, en ... | 2008 | 18620976 |
| genetic and physiological diversity of tetragenococcus halophilus strains isolated from sugar- and salt-rich environments. | tetragenococcus halophilus is known to flourish in extreme salt environments. recently, this halophilic bacterium also appeared as the dominant microflora during storage of sugar thick juice, an intermediate product of beet sugar production. although t. halophilus can cause degradation of thick juice, dominance of this bacterium does not always result in degradation. in this study t. halophilus strains from high-salt and high-sugar environments, and in particular from degraded and non-degraded t ... | 2008 | 18757794 |
| structural and functional importance of transmembrane domain 3 (tm3) in the aspartate:alanine antiporter aspt: topology and function of the residues of tm3 and oligomerization of aspt. | aspt, the aspartate:alanine antiporter of tetragenococcus halophilus, a membrane protein of 543 amino acids with 10 putative transmembrane (tm) helices, is the prototype of the aspartate:alanine exchanger (aae) family of transporters. because tm3 (isoleucine 64 to methionine 85) has many amino acid residues that are conserved among members of the aae family and because tm3 contains two charged residues and four polar residues, it is thought to be located near (or to form part of) the substrate t ... | 2009 | 19181816 |
| clinical efficacy of halophilic lactic acid bacterium tetragenococcus halophilus th221 from soy sauce moromi for perennial allergic rhinitis. | recently, some common foods in daily life, especially lactic acid bacteria, have been found to have anti-allergic effects. we previously isolated a halophilic lactic acid bacterium, tetragenococcus halophilus th221, from soy sauce moromi, a mixture of koji and salt solution, and showed that it possesses an immunomodulatory activity that promotes t helper type 1 immunity. | 2009 | 19240374 |
| analysis of microbial communities in doenjang, a korean fermented soybean paste, using nested pcr-denaturing gradient gel electrophoresis. | doenjang is a traditional korean fermented soybean paste that provides a major source of protein. the microbial diversity of 10 samples of doenjang (5 commercially manufactured products and 5 homemade products) was investigated using nested pcr-denaturing gradient gel electrophoresis (dgge). in the first step, the nearly complete 16s rrna and 18s rrna genes were amplified using universal primers. subsequently, these products were used as a template in a nested pcr to obtain fragments suitable fo ... | 2009 | 19324443 |
| analysis of bacterial and fungal communities in japanese- and chinese-fermented soybean pastes using nested pcr-dgge. | the microbial diversity of japanese- and chinese-fermented soybean pastes was investigated using nested pcr-denaturing gradient gel electrophoresis (dgge). five japanese-fermented soybean paste samples and three chinese-fermented soybean paste samples were analyzed for bacteria and fungi. extracted dna was used as a template for pcr to amplify 16s rrna and 18s rrna genes. the nearly complete 16s rrna and 18s rrna genes were amplified using universal primers, and the resulting products were subse ... | 2010 | 19924476 |
| effect of halotolerant starter microorganisms on chemical characteristics of fermented chum salmon (oncorhynchus keta) sauce. | chum salmon sauce mash was inoculated with barley koji (barley steamed and molded with aspergillus oryzae ) and halotolerant microorganisms (htms), zygosaccharomyces rouxii , candida versatilis , and tetragenococcus halophilus , in nine different combinations under non-aseptic conditions similar to the industrial fish sauce production and fermented at 35 +/- 2.5 degrees c for 84 days. the changes in the chemical components, color, and sensory properties during fermentation were investigated. fre ... | 2010 | 20405947 |
| microbiota during fermentation of chum salmon (oncorhynchus keta) sauce mash inoculated with halotolerant microbial starters: analyses using the plate count method and pcr-denaturing gradient gel electrophoresis (dgge). | nine different combinations of mugi koji (barley steamed and molded with aspergillus oryzae) and halotolerant microorganisms (htms), zygosaccharomyces rouxii, candida versatilis, and tetragenococcus halophilus, were inoculated into chum salmon sauce mash under a non-aseptic condition used in industrial fish sauce production and fermented at 35 +/- 2.5 degrees c for 84 days to elucidate the microbial dynamics (i.e., microbial count and microbiota) during fermentation. the viable count of halotole ... | 2010 | 20417400 |
| proteinase-producing halophilic lactic acid bacteria isolated from fish sauce fermentation and their ability to produce volatile compounds. | halophilic lactic acid bacteria were isolated from fish sauce mashes fermented at 1 to 12 months. seven out of sixty-four isolates were selected according to their proteolytic activity and growth at 25% nacl for characterization and investigation of volatile compound production. all selected isolates were gram-positive cocci with pairs/tetrads and grew at 0-25% nacl, ph 4.5-9.0. results of 16s rrna gene sequence analysis showed 99% homology to tetragenococcus halophilus atcc 33315. the restricti ... | 2010 | 20541276 |
| comparison of pcr-dgge and pcr-sscp analysis for bacterial flora of japanese traditional fermented fish products, aji-narezushi and iwashi-nukazuke. | the bacterial flora of two japanese traditional fermented fish products, aji-narezushi (salted and long-fermented horse mackerel (trachurus japonicas) with rice) and iwashi-nukazuke (salted and long-fermented sardine (sardinops melanostica) with rice bran), was analysed using non-culture-based polymerase chain reaction (pcr) denaturing gradient gel electrophoresis (dgge) and culture-based pcr single-strand conformation polymorphism (sscp) methods. | 2010 | 20572057 |
| isolation and partial characterization of halotolerant lactic acid bacteria from two mexican cheeses. | isolated strains of halotolerant or halophilic lactic acid bacteria (halab) from cotija and doble crema cheeses were identified and partially characterized by phenotypic and genotypic methods, and their technological abilities were studied in order to test their potential use as dairy starter components. humidity, a(w), ph, and salt concentration of cheeses were determined. genotypic diversity was evaluated by randomly amplified polymorphic dna-polymerase chain reaction. molecular identification ... | 2011 | 21327742 |
| method for rapid and sensitive detection of enterococcus sp. and enterococcus faecalis/faecium cells in potable water samples. | we have developed a rapid and robust technological solution including a membrane filtration and dissolution method followed by a molecular enrichment and a real-time pcr assay, for detecting the presence of enterococcus sp. or enterococcus faecalis/faecium per 100 ml of water in less than 5 h and we compared it to method 1600 on mei agar in terms of specificity, sensitivity, and limit of detection. the mei and the enterococcus sp.-specific assay detected respectively 73 (64.0%) and 114 (100%) of ... | 2011 | 21333318 |
| characterization of tetragenococcus strains from sugar thick juice reveals a new species, tetragenococcus osmophilus sp. nov., and divides tetragenococcus halophilus into two subspecies, t. halophilus subsp. halophilus subsp. nov. and t. halophilus subsp. flandriensis subsp. nov. | most bacteria recovered so far from sugar thick juice during storage represented strains of the species tetragenococcus halophilus. recently, several gram-positive, non-motile, non-sporeforming cocci with other physiological and genetic traits were isolated from sugar thick juice samples from different origin. in this study, representative isolates were investigated using a polyphasic taxonomic approach. the 16s rrna gene sequence similarity between these isolates and their closest relative, tet ... | 2011 | 21357458 |
| diversity of plasmids encoding histidine decarboxylase gene in tetragenococcus spp. isolated from japanese fish sauce. | nineteen isolates of histamine producing halophilic bacteria were isolated from four fish sauce mashes, each mash accumulating over 1000ppm of histamine. the complete sequences of the plasmids encoding the pyruvoyl dependent histidine decarboxylase gene (hdca), which is harbored in histamine producing bacteria, were determined. in conjunction, the sequence regions adjacent to hdca were analyzed to provide information regarding its genetic origin. as reference strains, tetragenococcus halophilus ... | 2011 | 21616548 |
| substrate specificity of the aspartate:alanine antiporter (aspt) of tetragenococcus halophilus in reconstituted liposomes. | the aspartate:alanine antiporter (aspt) of the lactic acid bacterium tetragenococcus halophilus is a member of the aspartate:alanine exchanger (aaex) transporter family. t. halophilus aspt catalyzes the electrogenic exchange of l-aspartate(1-) with l-alanine(0). although physiological functions of aspt were well studied, l-aspartate(1-):l-alanine(0) antiport mechanisms are still unsolved. here we report that the binding sites of l-aspartate and l-alanine are independently present in aspt by mean ... | 2011 | 21719707 |
| use of tetragenococcus halophilus as a starter culture for flavor improvement in fish sauce fermentation. | the potential of tetragenococcus halophilus as a starter culture for flavor improvement in fish sauce fermentation was elucidated. four strains of t. halophilus isolated from fish sauce mashes were inoculated to anchovy mixed with 25% nacl with an approximate cell count of 10(6) cfu/ml. the +¦-amino content of 6-month-old fish sauce samples inoculated with t. halophilus was 780-784 mm. the addition of t. halophilus mrc10-1-3 and t. halophilus mcd10-5-10 resulted in a reduction of histamine (p < ... | 2011 | 21710980 |
| a sulfated polysaccharide, fucoidan, enhances the immunomodulatory effects of lactic acid bacteria. | fucoidan, a sulfated polysaccharide contained in brown algae, has a variety of immunomodulatory effects, including antitumor and antiviral effects. on the other hand, lactic acid bacteria (lab) also have immunomodulatory effects such as anti-allergic effects. in this study, we demonstrated that fucoidan enhances the probiotic effects of lab on immune functions. by using peyer's patch cells and spleen cells in vitro, fucoidan amplified i ... | 2011 | 22160132 |