Publications

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post-meiotic cytokinesis and pollen aperture pattern ontogeny: comparison of development in four species differing in aperture pattern.pollen aperture patterns vary widely in angiosperms. an increasing number of studies indicate that aperture pattern ontogeny is correlated with the way in which cytokinesis that follows male meiosis is completed. the formation of the intersporal callose walls that isolate the microspores after meiosis was studied in four species with different aperture patterns (two monocots, phormium tenax and asphodelus albus, and two eudicots, helleborus foetidus and protea lepidocarpodendron). the way in whi ...200521652436
coumarins in phormium (new zealand flax) fibers: their role in fluorescence and photodegradation.the genus phormium (new zealand flax) has fiber possessing a high content of lignin and, like other lignocellulosic materials, it is subject to photodegradation. photoproducts in the fiber absorbing over a broad spectral region from ∼370 to 600nm are observed as a result of exposure to near-uv radiation from 350 to 400 nm. irradiation was shown to produce hydrogen peroxide and this can account, at least in part, for the photo-oxidation manifested in changes in the reflectance spectra of the fibe ...201121175642
structural diversity of fructans from members of the order asparagales in new zealand.the water-soluble carbohydrates (wscs) extracted from the underground parts of arthropodium cirratum, astelia banksii, bulbinella hookeri, dianella nigra and xeronema callistemon, and the flower stem of phormium tenax have been investigated. extracts of a. cirratum, b. hookeri, d. nigra and p. tenax contained 108.4, 28.1, 41.9 and 29.7 mg gfw(-1) wscs, respectively. thin-layer chromatography (tlc) showed that these species contained fructans. extracts of a. banksii and x. callistemon each contai ...200312737984
sequence heterogeneity in the two 16s rrna genes of phormium yellow leaf phytoplasma.phormium yellow leaf (pyl) phytoplasma causes a lethal disease of the monocotyledon, new zealand flax (phormium tenax). the 16s rrna genes of pyl phytoplasma were amplified from infected flax by pcr and cloned, and the nucleotide sequences were determined. dna sequencing and southern hybridization analysis of genomic dna indicated the presence of two copies of the 16s rrna gene. the two 16s rrna genes exhibited sequence heterogeneity in 4 nucleotide positions and could be distinguished by the re ...19968795200
antifungal and other compounds isolated from the roots of new zealand flax plants (the genus phormium). 19873430174
functional plant cell wall design revealed by the raman imaging approach.using the raman imaging approach, the optimization of the plant cell wall design was investigated on the micron level within different tissue types at different positions of a phormium tenax leaf. pectin and lignin distribution were visualized and the cellulose microfibril angle (mfa) of the cell walls was determined. a detailed analysis of the raman spectra extracted from the selected regions, allowed a semi-quantitative comparison of the chemical composition of the investigated tissue types on ...201121197544
a preliminary study of the polyuronide hemicellulose of phormium tenax (n.z.flax). 194521008352
indigenous ancestral sayings contribute to modern conservation partnerships: examples using phormium tenax.traditional ecological knowledge (tek) is central to indigenous worldviews and practices and is one of the most important contributions that indigenous people can bring to conservation management partnerships. however, researchers and managers may have difficulty accessing such knowledge, particularly where knowledge transmission has been damaged. a new methodological approach analyzes ancestral sayings from maori oral traditions for ecological information about phormium tenax, a plant with high ...200919323188
an evaluation of anthelmintic properties, assessed using faecal nematode egg counts, of new zealand native flax (phormium tenax).to investigate the anthelmintic properties of new zealand native flax (phormium tenax) for cattle, using a faecal egg count reduction test (fecrt).200819043474
species of colletotrichum on agavaceae.species of colletotrichum cause diseases on a wide range of hosts, frequently infecting plants in the agavaceae (monocotyledons: liliales). three species of colletotrichum restricted to the agavaceae were detected through morphological studies of specimens and molecular sequence analyses of the lsu of the nu-rdna and the its region of the nu-rdna from cultures. colletotrichum agaves on agave is fully described and illustrated. colletotrichum dracaenophilum is described as a new species for isola ...200617137776
comparative analysis of the plasmids from two isolates of "candidatus phytoplasma australiense".two plasmids from the plant-pathogenic mollicute "candidatus phytoplasma australiense" were completely sequenced from two isolates derived from different plant hosts. plasmid ppaph2 (3607bp) was obtained from phormium showing phormium yellow leaf symptoms and ppasb11 (3635bp) from strawberry showing strawberry lethal yellows symptoms. the plasmids varied in their copy number and nucleotide sequence yet contained the same four open reading frames (orfs). the deduced amino acid sequence derived fr ...200616620976
methods for characterizing plant fibers.the effectiveness of different microscopy techniques for measuring the dimensions of ultimate fibers from harakeke (phormium tenax, new zealand flax) was investigated using a factorial experimental design. constant variables were geographical location, location of specimens along the leaf, season (winter), individual plant, a fourth leaf from a north-facing fan, age of plant, and cultivars (two). experimental variables were microscopy techniques and measurement axis. measurements of width and le ...200516170820
the controversial telomeres of lily plants.the molecular structure of the exceptional telomeres of six plant species belonging to the order asparagales and two species of the order liliales was analyzed using southern blot and fluorescence in situ hybridization. three different situations were found, namely: i) in the two liliales species, tulipa australis (liliaceae) and merendera montana (colchicaceae), the chromosome ends display hybridization signals with oligonucleotides resembling telomere repeats of both plants (tttaggg)n and vert ...200515753570
structure of fructans from excised leaves of new zealand flax.the accumulation of total water-soluble carbohydrate, and specifically sucrose and fructan, by excised leaves of phormium tenax and p. cookianum (family phormiaceae j. g. agardh, order asparagales) was investigated. total water-soluble carbohydrate content of excised leaves of p. tenax and p. cookianum increased during 48 h of continuous illumination at an average rate of 1.3 and 0.9 mg g(-1) fresh weight leaf per hour, respectively. the sucrose content of excised leaves increased throughout the ...200111397431
'candidatus phytoplasma japonicum', a new phytoplasma taxon associated with japanese hydrangea phyllody.a phytoplasma was discovered in diseased specimens of field-grown hortensia (hydrangea spp.) exhibiting typical phyllody symptoms. pcr amplification of dna using phytoplasma specific primers detected phytoplasma dna in all of the diseased plants examined. no phytoplasma dna was found in healthy hortensia seedlings. rflp patterns of amplified 16s rdna differed from the patterns previously described for other phytoplasmas including six isolates of foreign hortensia phytoplasmas. based on the rflp, ...199910425791
phylogenetic positions of phytoplasmas associated with dieback, yellow crinkle and mosaic diseases of papaya, and their proposed inclusion in 'candidatus phytoplasma australiense' and a new taxon, 'candidatus phytoplasma australasia'.dna extracted from three papaya (carica papaya l.) plants, individually affected by dieback, yellow crinkle or mosaic diseases, was subjected to pcr using phytoplasma-specific primers to amplify the 16s rrna gene plus 16s-23s rrna intergenic spacer region. near-complete dna sequences obtained for the three pcr amplimers were subjected to phylogenetic analyses and direct sequence comparison with other phytoplasma 16s rdna and 16s-23s spacer region dna sequences. the papaya yellow crinkle (ppyc) a ...19989734050
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