| analysis of 16s rrna gene sequences of vibrio costicola strains: description of salinivibrio costicola gen. nov., comb. nov. | the phylogenetic positions of six vibrio costicola strains were determined by direct sequencing and analysis of their pcr-amplified 16s ribosomal dnas. a comparative analysis of the sequence data revealed that the moderate halophile v. costicola forms a monophyletic branch that is distinct from other vibrio species and from moderately halophilic species of other genera. these results complement phenotypic and genotypic data determined previously. the molecular evidence, together with several phe ... | 1996 | 8782695 |
| salinivibrio costicola subsp. vallismortis subsp. nov., a halotolerant facultative anaerobe from death valley, and emended description of salinivibrio costicola. | strain dvt, a halotolerant, gram-negative, facultatively anaerobic bacterium, was isolated from a hypersaline pond located in death valley, california. the cells were non-spore-forming, motile, curved rods (1.0-1.8 x 0.5-0.6 microns) and occurred singly, in pairs or rarely in chains. strain dvt was oxidase-, catalase-, voges-proskauer-, amylase-, gelatinase- and lipase-positive and indole-negative. nitrate, sulfate and fumarate were not used as electron acceptors. carbohydrates served as energy ... | 2000 | 10758867 |
| polyamine distribution patterns within the families aeromonadaceae, vibrionaceae, pasteurellaceae, and halomonadaceae, and related genera of the gamma subclass of the proteobacteria. | polyamines of the four families and the five related genera within the gamma subclass of the class proteobacteria were analyzed by hplc with the objective of developing a chemotaxonomic system. the production of putrescine, diaminopropane, cadaverine, and agmatine are not exactly correlated to the phylogenetic genospecies within 36 strains of the genus aeromonas (the family aeromonadaceae) lacking in triamines. the occurrence of norspermidine was limited but not ubiquitous within the family vibr ... | 1997 | 12501353 |
| diversity of moderately halophilic bacteria producing extracellular hydrolytic enzymes. | the aim of this study was to determine the diversity of moderately halophilic bacteria with hydrolase activities. | 2003 | 12534822 |
| screening and characterization of the protease cp1 produced by the moderately halophilic bacterium pseudoalteromonas sp. strain cp76. | a total of 26 proteolytic moderate halophiles were isolated and characterized. most isolates were members of the genus salinivibrio (16 strains), while others were identified as bacillus (4 strains), salinicoccus (2 strains), or members of the gamma-proteobacteria (4 strains). strain cp76 was selected as the best producer of an extracellular protease, designated cp1, and was used for further studies. sequence analysis of the 16s rrna gene in addition to phenotypic tests led to the placement of t ... | 2003 | 12768453 |
| halotolerant aerobic heterotrophic bacteria from the great salt plains of oklahoma. | the salt plains national wildlife refuge (spnwr) near cherokee, oklahoma, contains a barren salt flat where permian brine rises to the surface and evaporates under dry conditions to leave a crust of white salt. rainfall events dissolve the salt crust and create ephemeral streams and ponds. the rapidly changing salinity and high surface temperatures, salinity, and uv exposure make this an extreme environment. the salt plains microbial observatory (spmo) examined the soil microbial community of th ... | 2004 | 15696379 |
| salinivibrio costicola subsp. alcaliphilus subsp. nov., a haloalkaliphilic aerobe from campania region (italy). | phenotypic and phylogenetic studies were performed on unidentified gram-negative staining, haloalkaliphilic aerobe and protease producer salinivibrio-like organism recovered from a saltish spring with algal mat in the "pozzo del sale" site (salt's well) in the campania region (south italy). phylogenetic analysis based on comparison of 16s rrna gene sequences demonstrated that the isolate was related to species of salinivibrio genus. the dna-dna hybridization of the type strain 18ag(t) with the m ... | 2005 | 15709363 |
| the diversity of culturable organotrophic bacteria from local solar salterns. | we isolated and cultured bacteria inhabiting solar saltern ponds in taean-gun, chungnam province, korea. all of the isolated 64 strains were found to be moderately halophilic bacteria, growing in a salt range of 2-20 %, with an optimal concentration of 5% salt. bacterial diversity among the isolated halophiles was evaluated via rflp analyses of pcr-amplified 16s rdnas, followed by phylogenetic analysis of the partial 16s rdna sequences. the combination of restriction enzyme digestions with haeii ... | 2005 | 15765050 |
| purification and characterization of a protease produced by an aerobic haloalkaliphilic species belonging to the salinivibrio genus. | an extracellular protease produced at the end of the exponential growth phase was purified to homogeneity and characterized from the new isolate haloalkaliphilic strain 18ag, phylogenetically related to salinivibrio costicola subsp. costicola. the protease molecular mass was about 38 kda. the enzyme was dependent on salt concentration for activity and stability, and it showed optimal activity at 60 degrees c in the presence of 2.0% nacl and 2.0 mm cacl2, while in the absence of cacl2 the optimum ... | 2005 | 15862445 |
| cloning and characterization of the constitutively expressed chitinase c gene from a marine bacterium, salinivibrio costicola strain 5sm-1. | the chitinase c gene (chic) encoding chitinase c (chic) from salinivibrio costicola 5sm-1 was cloned and the nucleotide sequence was determined. s. costicola chic was expressed constitutively and repressed by glucose. a single operon composed of two complete open reading frames organized in the order of chib, chic and one partial open reading frame of chia was found in the same transcriptional direction. chic was composed of 2610 bp encoding for 870 amino acids with a calculated molecular mass o ... | 2003 | 16233569 |
| production of an extracellular alkaline metalloprotease from a newly isolated, moderately halophile, salinivibrio sp. strain af-2004. | an extracellular protease was produced under stress conditions of high temperature and high salinity by a newly isolated moderate halophile, salinivibrio sp. strain af-2004 in a basal medium containing peptone, beef extract, glucose and nacl. a modification of kunitz method was used for protease assay. the isolate was capable of producing protease in the presence of sodium chloride, sodium sulfate, sodium nitrate, sodium nitrite, potassium chloride, sodium acetate and sodium citrate. the maximum ... | 2007 | 16638631 |
| purification and biochemical characterization of a protease secreted by the salinivibrio sp. strain af-2004 and its behavior in organic solvents. | a metalloprotease secreted by the moderately halophilic bacterium salinivibrio sp. strain af-2004 when the culture reached the stationary growth phase. this enzyme was purified to homogeneity by acetone precipitation and subsequent q-sepharose anion exchange and sephacryl s-200 gel filtration chromatography. the apparent molecular mass of the protease was 31 kda by sds-page, whereas it was estimated as approximately 29 kda by sephacryl s-200 gel filtration. the purified protease had a specific a ... | 2007 | 17072685 |
| inferring the evolutionary history of vibrios by means of multilocus sequence analysis. | we performed the first broad study aiming at the reconstruction of the evolutionary history of vibrios by means of multilocus sequence analysis of nine genes. overall, 14 distinct clades were recognized using the splitstree decomposition method. some of these clades may correspond to families, e.g., the clades salinivibrio and photobacteria, while other clades, e.g., splendidus and harveyi, correspond to genera. the common ancestor of all vibrios was estimated to have been present 600 million ye ... | 2007 | 17704223 |
| reclassification of vibrio fischeri, vibrio logei, vibrio salmonicida and vibrio wodanis as aliivibrio fischeri gen. nov., comb. nov., aliivibrio logei comb. nov., aliivibrio salmonicida comb. nov. and aliivibrio wodanis comb. nov. | four closely related species, vibrio fischeri, vibrio logei, vibrio salmonicida and vibrio wodanis, form a clade within the family vibrionaceae; the taxonomic status and phylogenetic position of this clade have remained ambiguous for many years. to resolve this ambiguity, we tested these species against other species of the vibrionaceae for phylogenetic and phenotypic differences. sequence identities for the 16s rrna gene were > or =97.4 % among members of the v. fischeri group, but were < or =9 ... | 2007 | 18048732 |
| molecular cloning and sequence analysis of a novel zinc-metalloprotease gene from the salinivibrio sp. strain af-2004 and its extracellular expression in e. coli. | in this work the first protease gene encoding a novel zinc-metalloprotease from the moderately halophilic bacterium salinivibrio sp. strain af-2004 has been cloned, sequenced and reported to the genbank. we have generated a library containing about 10,000 transformants whose screening yielded one clone harboring plasmid pbluescript with 3.6 kb inserted fragment (pbluesvp2) with positive caseinolytic activity. nucleotide sequence analysis of the selected clone revealed a single open reading frame ... | 2008 | 18093752 |
| salinivibrio proteolyticus sp. nov., a moderately halophilic and proteolytic species from a hypersaline lake in iran. | a novel moderately halophilic, gram-negative, curved-rod-shaped bacterium, designated strain af-2004t, was isolated from bakhtegan lake, a hypersaline lake with 17 % (w/v) total salt located in the southern region of iran. strain af-2004t was a facultative anaerobe, motile by one polar flagellum, non-sporulating and oxidase- and catalase-positive. it grew at salinities of 1-17 % (w/v) nacl, showing optimal growth at 5 % (w/v). growth occurred at 10.0-45.0 degrees c and ph 5.0-9.5, with optimum g ... | 2008 | 18450706 |
| production of an extracellular thermohalophilic lipase from a moderately halophilic bacterium, salinivibrio sp. strain sa-2. | fifty strains of moderately halophilic bacteria were isolated from various salty environments in iran. a strain designated as sa-2 was shown to be the best producer of extracellular lipase and was selected for further studies. biochemical and physiological characterization along with 16s rdna sequence analysis placed sa-2 in the genus salinivibrio. the optimum salt, ph, temperature and aeration for enzyme production were 0.1 m kcl, ph 8, 35 degrees c and 150 rpm, respectively. the enzyme product ... | 2008 | 18506896 |
| isolation and characterization of salt-sensitive mutants of the moderately halophilic bacterium salinivibrio costicola subsp. yaniae. | salinivibrio costicola subsp. yaniae is a moderately halophilic bacterium which can grow over a wide range of salinity. in response to external osmotic stress (1-3 m nacl), s. costicola subsp. yaniae can accumulate ectoine, glycine betaine, and glutamate as compatible solutes. we used suicide plasmids psup101 to introduce transposon tn1732 into s. costicola subsp. yaniae via escherichia coli sm10 mediated by conjugation. one tn1732-induced mutant, mu1, which was very sensitive to the external sa ... | 2008 | 18685202 |
| salinivibrio siamensis sp. nov., from fermented fish (pla-ra) in thailand. | a gram-negative, facultatively anaerobic, moderately halophilic bacterium, strain nd1-1(t), was isolated from fermented fish (pla-ra) in thailand. the cells were curved rods, motile and non-endospore-forming. the novel strain grew optimally at 37 degrees c, at ph 8 and in the presence of 9-10 % (w/v) nacl. the predominant respiratory lipoquinone was q-8. the major cellular fatty acids were c(16 : 0) and c(12 : 0). polar lipid analysis revealed the presence of phosphatidylethanolamine, phosphatid ... | 2009 | 19329624 |
| [phylogenetic diversity of cultivable bacteria associated with a sea anemone from coast of the naozhou island in zhanjiang, china]. | to understand the diversity of cultivable bacteria isolated from a sea anemone collected from the coastal water of the naozbou island in the leizhou bay on south china sea. | 2009 | 19445182 |
| [diversity of culturable bacteria associated with the sea urchin hemicentrotus pulcherrimus from naozhou island]. | to investigate the diversity of culturable bacteria isolated from the sea urchin hemicentrotus pulcherrimus collected from a tidal flat of naozhou island (20 degrees 52'n - 20 degrees 56'n 110 degrees 33'e - 110 degrees 38'e), leizhou bay, south china sea, china. | 2009 | 20112668 |
| enhanced activity and stability in the presence of organic solvents by increased active site polarity and stabilization of a surface loop in a metalloprotease. | salinivibrio zinc-metalloprotease (svp) is an enzyme which was isolated from salinivibrio proteolyticus, a moderately halophilic species from a hypersaline lake in iran. a195e and g203d mutants were constructed to increase polarity near the active site in order to preserve the hydration layer against organic solvents [dimethylformamide (dmf), methanol, isopropanol and n-propanol]. a268p was constructed to stabilize a surface loop far from the active site and a195e/a268p was constructed to invest ... | 2010 | 20519323 |
| accumulation and role of compatible solutes in fast-growing salinivibrio costicola subsp. yaniae. | the moderately halophilic bacterium salinivibrio costicola subsp. yaniae showed an extremely fast growth rate. optimal growth was observed in artificial seawater containing 1.4 mol/l nacl and in mm63 media containing 0.6 mol/l nacl. we analyzed a variety of compatible solutes that had accumulated in this strain grown in the media. the supplementation effect of the compatible solutes glycine betaine, glutamate, and ectoine to the growth of s. costicola subsp. yaniae was examined. glycine betaine ... | 2010 | 21164572 |
| salinivibrio sharmensis sp. nov., a novel haloalkaliphilic bacterium from a saline lake in ras mohammed park (egypt). | a novel haloalkaliphilic, facultative anaerobic and gram-negative salinivibrio-like microorganism (designated strain bag(t)) was recovered from a saline lake in ras mohammed park (egypt). cells were motile, curved rods, not spore-forming and occurred singly. strain bag(t) grew optimally at 35°c (temperature growth range 25-40°c) with 10.0% (w/v) nacl [nacl growth range 6.0-16.0% (w/v)] and at ph 9.0 (ph growth range 6.0-10.0). strain bag(t) had phosphatidylethanolamine (pea) and phosphatidylglyc ... | 2011 | 21287211 |
| detection of quorum sensing signal molecules in the family vibrionaceae. | the aim of this study was to detect the production of three kinds of quorum sensing (qs) signal molecules, i.e. the n-acyl-homoserine lactone (ahl), the autoinducer-2 (ai-2) and the cholerae autoinducer-1-like (cai-1-like) molecules in 25 vibrionaceae strains. | 2011 | 21395950 |
| bacteria associated with artemia spp. along the salinity gradient of the solar salterns at eilat (israel). | the crustacean genus artemia naturally inhabits various saline and hypersaline environments and is the most frequently laboratory-hatched animal for live feed in mari- and aquaculture. because of its high economic importance, artemia-bacteria interactions were so far studied mostly in laboratory strains. in this study, we focused our attention on the artemia-associated microbiota in its natural environment in the solar salterns of eilat, israel. we applied a culture-independent method (clone lib ... | 2011 | 21492196 |