| virus-independent and common transcriptome responses of leafhopper vectors feeding on maize infected with semi-persistently and persistent propagatively transmitted viruses. | insects are the most important epidemiological factors for plant virus disease spread, with >75% of viruses being dependent on insects for transmission to new hosts. the black-faced leafhopper (graminella nigrifrons forbes) transmits two viruses that use different strategies for transmission: maize chlorotic dwarf virus (mcdv) which is semi-persistently transmitted and maize fine streak virus (mfsv) which is persistently and propagatively transmitted. to date, little is known regarding the molec ... | 2014 | 24524215 |
| detection of members of the secoviridae in the tallgrass prairie preserve, osage county, oklahoma, usa. | viruses are most frequently discovered because they cause disease. to expand knowledge of plant-associated viruses beyond these narrow constraints, non-cultivated plants of the tallgrass prairie of the united states were systematically surveyed for evidence of viruses. this report discusses putative viruses of the family secoviridae identified by the survey. sequence analysis suggests the presence of at least six viruses in the study site, including bean pod mottle virus, maize chlorotic dwarf v ... | 2012 | 22487310 |
| viruses in maize and johnsongrass in southern ohio. | the two major u.s. maize viruses, maize dwarf mosaic virus (mdmv) and maize chlorotic dwarf virus (mcdv), emerged in southern ohio and surrounding regions in the 1960s and caused significant losses. planting resistant varieties and changing cultural practices has dramatically reduced virus impact in subsequent decades. current information on the distribution, diversity, and impact of known and potential u.s. maize disease-causing viruses is lacking. to assess the current reservoir of viruses pre ... | 2014 | 24918609 |
| genetic analysis of resistance to six virus diseases in a multiple virus-resistant maize inbred line. | novel and previously known resistance loci for six phylogenetically diverse viruses were tightly clustered on chromosomes 2, 3, 6 and 10 in the multiply virus-resistant maize inbred line, oh1vi. virus diseases in maize can cause severe yield reductions that threaten crop production and food supplies in some regions of the world. genetic resistance to different viruses has been characterized in maize populations in diverse environments using different screening techniques, and resistance loci hav ... | 2014 | 24500307 |
| identification of a maize chlorotic dwarf virus silencing suppressor protein. | maize chlorotic dwarf virus (mcdv), a member of the genus waikavirus, family secoviridae, has a 11784 nt (+)ssrna genome that encodes a 389kda proteolytically processed polyprotein. we show that the n-terminal 78kda polyprotein (r78) of mcdv acts as a suppressor of rna silencing in a well-established assay system. we further demonstrate that r78 is cleaved by the viral 3c-like protease into 51 and 27kda proteins (p51 and p27), and that p51 is responsible for silencing suppressor activity. silenc ... | 2017 | 28160664 |
| identification of quantitative trait loci controlling resistance to maize chlorotic dwarf virus. | ineffective screening methods and low levels of disease resistance have hampered genetic analysis of maize (zea mays l.) resistance to disease caused by maize chlorotic dwarf virus (mcdv). progeny from a cross between the highly resistant maize inbred line oh1vi and the susceptible inbred line va35 were evaluated for mcdv symptoms after multiple virus inoculations, using the viral vector graminella nigrifrons. symptom severity scores from three rating dates were used to calculate area under the ... | 2004 | 15551042 |
| effect of environmental conditions and leafhopper gender on maize chlorotic dwarf virus transmission by graminella nigrifrons (homoptera: cicadellidae). | to determine the most economical and efficient means to maintain cultures of maize chlorotic dwarf virus (mcdv) and to screen for host plant resistance to mcdv, we evaluated the effects of temperature, light intensity, daylength, atmospheric pressure, and leafhopper gender on the frequency of transmission of mcdv by grarminella nigrifrons forbes (homoptera: cicadellidae). female leafhoppers transmitted at higher frequencies than males under most conditions. in temperature studies, transmission r ... | 2004 | 15279251 |
| accumulation of maize chlorotic dwarf virus proteins in its plant host and leafhopper vector. | the genome of maize chlorotic dwarf virus (mcdv; genus waikavirus; family sequiviridae) consists of a monopartite positive-sense rna genome encoding a single large polyprotein. antibodies were produced to his-fusions of three undefined regions of the mcdv polyprotein: the n-terminus of the polyprotein (r78), a region between coat proteins (cps) and the nucleotide-binding site (nbs) (r37), and a region between the nbs and a 3c-like protease (r69). the r78 antibodies react with proteins of 50 kda ... | 2004 | 15246276 |
| nucleotide sequence and taxonomy of maize chlorotic dwarf virus within the family sequiviridae. | the complete sequence of a tennessee isolate of maize chlorotic dwarf virus (mcdv-tn) was determined from cdna clones and by direct sequencing of the viral rna. the genome is 11813 nucleotides (nt) in length and contains one large open reading frame between nt 435 and 10763 that encodes a polyprotein of 3443 amino acids. the n-terminal amino acid sequences were determined for the three capsid proteins. all three were adjacent, starting at nt 2526 and putatively ending at nt 3761. comparison of t ... | 1997 | 9152437 |
| properties of maize chlorotic dwarf virus and its ribonucleic acid. | | 1976 | 960565 |