| herbicidins c and e, two new necleoside antibiotics. | streptomyces saganonensis no. 4075 used for production of herbicidins a and b has also produced other two antibiotics, herbicidins c and e in submerged culture. isolation of the antibiotics was performed by adsorption on resinous adsorbent followed by elution with aqueous meoh and completed by silica-gel chromatography. physico-chemical characteriation revealed that herbicidins c and e are new nucleoside antibiotics having an adenine moiety in their structures. herbicidins c and e indicated inhi ... | 1979 | 117003 |
| [the effect of actinomycin d on the production of viruses by the protoplasts of chinese cabbage infected in vitro by the yellow mosaic virus of turnips]. | chinese cabbage (brassica sinensis l. var. cantonner) protoplasts were infected by turnip yellow mosaic virus (tymv) and inoculated in the presence or absence of actinomycin d. virus production was determined 40 hrs. after inoculation, the time required for the virus replication cycle to be terminated. while actinomycin d had no effect on tymv production when present at a concentration of 1 microng/ml, a 50 to 80% inhibition of virus production was noticed at concentrations of the order of 5 to ... | 1977 | 405160 |
| populations of the spinach wilt pathogen, fusarium oxysporum f. sp. spinaciae, in the root tissues, rhizosphere, and soil in the field. | populations of fusarium oxysporum f. sp. spinaciae in root tissues and rhizosphere soil of diseased spinach plants were higher than in the root tissues and rhizosphere soil of healthy plants. populations in soil rhizosphere were higher than in nonrhizosphere soil. the fungus populations were very low in the root tissues of the nonsusceptible strawberry, broccoli, chinese cabbage, and mustard grown in the infested field. the populations were low at the beginning of the season, increased, and rema ... | 1979 | 436018 |
| synthesis and accumulation of polyamines and s-adenosylmethionine in chinese cabbage infected by turnip yellow mosaic virus. | | 1979 | 465028 |
| structure of plant nuclear and ribosomal dna containing chromatin. | digestion of plant chromatin from brassica pekinensis and matthiola incana with staphylococcus nuclease leads to a dna repeat of 175 plus or minus 8 and a core size of 140 base pairs. dnase i digestion results in multiples of 10 bases. ribosomal rnn genes were studied as a model system for active plant chromatin because of their great redundancy and their high transcriptional activity in growing and differentiating tissues. the actively transcribed genes were identified by nascent rna of ribosom ... | 1979 | 514813 |
| [in vitro infection of chinese cabbage protoplasts by the turnip yellow mosaic virus]. | | 1975 | 808296 |
| isolation of viruses by electro-extraction of infected plants. | the isolation of viruses from infected plant material by a process termed electro-extraction appeared to be a convenient and simple method of obtaining viruses in a fair state of purity. the method has the advantage over the conventional methods of virus purification that the infected plant tissue is not disintegrated and that organic solvents such as chloroform and butanol are avoided. the procedure used was demonstrated on the extraction of tobacco mosaic virus (tmv) from infected tobacco and ... | 1977 | 909872 |
| inhibition of turnip yellow mosaic virus synthesis by pyrimidine analogues. | the pyrimidine analogues 2-thiouracil, 2-thiouridine, 6-azauracil and 6-azauridine all inhibited the synthesis of turnip yellow mosaic virus (tymv) and increased the synthesis of empty virus protein shells in infected chinese cabbage leaf discs. uracil and uridine reversed these effects. 2-thiouracil also reduced the utp pool in tymv infected leaf discs. the results are consistent with the suggestion that these analogues or their in vivo derivatives affect virus synthesis by inhibiting the biosy ... | 1976 | 989025 |
| herbicidins a and b, two new antibiotics with herbicidal activity. i. producing organism and biological activities. | herbicidins a and b, two new antibiotics with selective and contact herbicidal activity, were produced by a new species of streptomyces designated as s. sagononensis. among tested microorganisms, herbicidins indicated some activity against fungi in vitro and xanthomonas oryzae in vivo. their characteristic features were brought into relief by their selective and contact killing effect on many dicotyledonous plant as well as their inhibition of germination of the plant seeds, such as chinese cabb ... | 1976 | 993127 |
| location, rate and asymmetry of ds-rna synthesis during replication of tymv in chinese cabbage. | the ds-rna induced by tymv infection in the nuclei of infected chinese cabbage leaf cells became labelled with [32p]phosphate most rapidly before production of virus could be detected. in contrast, the ds-rna associated with the chloroplasts became labelled most rapidly during the period of maximum virus production. | 1976 | 1275406 |
| localization of cauliflower mosaic virus in the cell nucleus of brassica pekinensis l. | cauliflower mosaic virus (camv) particles were observed in the nuclei of xylem parenchyma cells in brassica pekinensis l. doubly infected by camv and turnip mosaic virus (tumv). camv particles were aggregated in the nucleoplasm but not embedded in viroplasms. this phenomenon was not detected in cell nuclei of mesophyll tissue. typical features associated with infection by either camv or tumv normally occurred in the cytoplasm of cells of both tissues: two types of viroplasms with embedded camv p ... | 1992 | 1410828 |
| turnip yellow mosaic virus variants produced from dna clones encoding their genomes. | full-length dsdna clones that encode the genomes of two australian turnip yellow mosaic isolates, tymv-bl and tymv-cl have been constructed. these clones were transcribed to give 6.3 kb capped ssrna which infects chinese cabbages to give symptoms indistinguishable from those produced by the parental viruses. extensions of up to 26 nucleotides at the 3' end of the tymv-bl clone delay infections, but virus particles isolated from these plants 4 weeks after inoculation contain rna with the original ... | 1992 | 1456892 |
| second-site suppressor mutations assist in studying the function of the 3' noncoding region of turnip yellow mosaic virus rna. | the 3' noncoding region of turnip yellow mosaic virus rna includes an 82-nucleotide-long trna-like structure domain and a short upstream region that includes a potential pseudoknot overlapping the coat protein termination codon. genomic rnas with point mutations in the 3' noncoding region that result in poor replication in protoplasts and no systemic symptoms in planta were inoculated onto chinese cabbage plants in an effort to obtain second-site suppressor mutations. putative second-site suppre ... | 1992 | 1501271 |
| [nucleic acid components in carbohydrate-rich food]. | the content of nucleic acid components in numerous foods, especially carbohydrate-rich ones, has been investigated. the data obtained for bases (purines and pyrimidines) were calculated as nucleic-acid equivalents (rna or dna); the imp content was calculated from the measured content of hypoxanthine. not only did cultivated plants such as cereals and pulses show a high rna-equivalent content but also vegetables such as spinach, leek, broccoli, chinese cabbage and cauliflower. we found the same r ... | 1990 | 1689090 |
| expression of orf-69 of turnip yellow mosaic virus is necessary for viral spread in plants. | turnip yellow mosaic virus (tymv) rna has two extensively overlapping open reading frames (orfs) encoding noncapsid proteins. the longest of these, orf-206, is essential for rna replication. we have investigated the expression and role of orf-69 (encoding a protein with a mw of 69k) using specific antiserum and mutant genomic rnas designed to interrupt orf-69 expression. tyc69 antiserum immunoprecipitated a protein with an apparent mw of 75k (p69) from in vitro translations of tymv rna. mutant r ... | 1992 | 1736523 |
| turnip yellow mosaic virus rnas with anticodon loop substitutions that result in decreased valylation fail to replicate efficiently. | single and multiple nucleotide substitutions have been introduced into the anticodon loop of the trna-like structure of turnip yellow mosaic virus (tymv) genomic rna. we studied the effects of these mutations on in vitro valylation and on replication in chinese cabbage protoplasts and plants. only those mutants capable of efficient and complete valylation showed efficient replication in protoplasts and gave rise to systemic symptoms in whole plants. mutants that accepted valine inefficiently (in ... | 1991 | 2033666 |
| first isolation of xanthomonas campestris from the blood of a chinese woman. | xanthomonas campestris isolated from the blood of a patient with a fever was first reported. xanthomonas campestris is a bacterium that can cause black rot of some vegetables, such as rape. chinese cabbage, etc. human infection due to x. campestris has not been reported so far. the characteristics of this organism, including morphology, staining, physiology and biochemistry were studied. we believe that x. campestris is also one of the opportunistic pathogens, which can infect compromised host. | 1990 | 2118062 |
| induction of nitroarenes in cigarette smoke condensate treated with nitrate. | samples of cigarette smoke condensates (cscs) treated with nitric acid or chinese cabbage pickles having a high nitrate content strongly mutated salmonella typhimurium strain ta98; the benzene/ethanol extract after these treatments induced 1800 and 820 revertants, respectively, per mg of extract for strain ta98 in the absence of s9 mix. the major mutagens in these materials were found to be 1-nitropyrene and 1,3-dinitropyrene on the basis of the result of gas chromatography/mass spectrometry and ... | 1990 | 2155483 |
| [antimutagenic activity of vegetable and fruit extracts against in-vitro benzo(a)pyrene]. | about 80% of the juices from twenty vegetables and fruits showed antimutagenic activity when tested in the presence of the mutagen and carcinogen benzo[a]pyrene using the ames' salmonella/microsome assay. in a standardized test system juices from raw celeriac, broccoli, red cabbage, carrots, green, peppers, lettuce, asparagus, apricots, red-currants, gooseberries, raspberries, and pineapple showed more than 50% inhibition. leek, kohlrabi, cucumber, zucchini, french beans, fennel leaves, rhubarb, ... | 1990 | 2336853 |
| infectious tymv rna from cloned cdna: effects in vitro and in vivo of point substitutions in the initiation codons of two extensively overlapping orfs. | full-length cdna of the 6.3 kb turnip yellow mosaic virus (tymv) genome was placed between a t7 promoter and a unique hind iii site. in vitro transcription of hind iii-linearized dna of clone ptymc yielded full-length rna transcripts. in inoculations of chinese cabbage protoplasts and plants, capped transcripts and virion rna had similar specific infectivities and produced similar systemic symptoms. we have used the ptymc clone in studies of the expression of two overlapping open reading frames ... | 1989 | 2473440 |
| immunochemical properties of elongation factors 1 of plant origin. | elongation factors 1 (ef-1) have been isolated from different plants: wheat, yellow lupine, blue lupine, chinese cabbage and norway maple. antibodies for ef-1 from yellow lupine have been obtained in rabbits; antibodies for wheat ef-1 were elicited in mice. the immunological properties of ef-1 were assayed by the following methods: western blotting, double immunodiffusion and rocket immunoelectrophoresis. our results suggest that one antigenic site is similar for all plant elongation binding fac ... | 1988 | 3123226 |
| 4-methoxyindole derivatives as nitrosable precursors of mutagens in chinese cabbage. | two indole compounds isolated from fresh chinese cabbage were shown to be mutagen precursors that yielded direct-acting mutagens on treatment with nitrite. they were identified as 4-methoxyindole-3-acetonitrile and 4-methoxy-indole-3-aldehyde. when these compounds were treated with 50 mm nitrite at ph 3.0 for 1 h at 37 degrees c, 4-methoxyindole-3-acetonitrile induced 31,800 and 10,000 revertants of salmonella typhimurium ta100 and ta98, respectively, per mg of mutagen precursor in the absence o ... | 1986 | 3331681 |
| recently identified nitrite-reactive compounds in food: occurrence and biological properties of the nitrosated products. | various japanese foodstuffs are directly-acting mutagens in salmonella typhimurium ta100 after nitrite treatment. such mutagen precursors include tyramine and beta-carboline derivatives, isolated from soya sauce, and indole-3-acetonitrile, 4-methoxyindole-3-acetonitrile and 4-methoxyindole-3-aldehyde, isolated from fresh chinese cabbage. a mutagen produced from tyramine with nitrite was found to be 4-(2-aminoethyl)-6-diazo-2,4-cyclohexadienone (3-diazotyramine), and one produced from indole-3-ac ... | 1987 | 3679387 |
| a mutagen precursor in chinese cabbage, indole-3-acetonitrile, which becomes mutagenic on nitrite treatment. | after treatment with nitrite, chinese cabbage showed direct-acting mutagenicity on salmonella typhimurium ta100 inducing 3100 revertants per g. one of the mutagen precursors that became mutagenic after nitrite treatment was isolated, and identified as indole-3-acetonitrile. after treatment with nitrite, 1 mg of indole-3-acetonitrile induced 17 400 revertants of ta100 and 21 000 revertants of ta98 without s9 mix. | 1985 | 3889600 |
| nitrosatable precursors of mutagens in vegetables and soy sauce. | nitrosatable precursors of mutagens that show mutagenicity to salmonella typhimurium ta100 without s9 mix after treatment with nitrite at ph 3 were found in various foods. from chinese cabbage, three indole compounds, indole-3-acetonitrile, 4-methoxyindole-3-acetonitrile, and 4-methoxyindole-3-aldehyde, were identified as mutagen precursors. 1-methylindole and 2-methylindole, which are present in cigarette smoke showed strong mutagen precursor activity. escherichia coli wp2 uvra/pkm101 is more s ... | 1985 | 3916206 |
| the incorporation of radiolabeled polyamines and methionine into turnip yellow mosaic virus in protoplasts from infected plants. | turnip yellow mosaic virus contains large amounts of nonexchangeable spermidine and induces an accumulation of spermidine in infected chinese cabbage. by 7 days after inoculation, a majority of protoplasts isolated from newly emerging leaves stain with fluorescent antibody to the virus. these protoplasts contain 1-2 x 10(6) virions per cell and continue to produce virus in culture for at least 48 hr. [14c]spermidine (10 microm) was taken up by these cells in amounts comparable to the original en ... | 1985 | 4060587 |
| the effects of dicyclohexylamine on polyamine biosynthesis and incorporation into turnip yellow mosaic virus in chinese cabbage protoplasts infected in vitro. | we have reported (r. balint and s. s. cohen, 1985, virology 144, 181-193) that protoplasts from plants infected with turnip yellow mosaic virus (tymv) continue to produce virus in culture and that newly formed virus particles contained predominantly newly synthesized spermidine and spermine. inhibition of spermidine synthesis by dicyclohexylamine (dcha), however, led to incorporation of preexisting spermidine and increased amounts of spermine into newly formed virions. we now report similar resu ... | 1985 | 4060588 |
| evidence for modification of protein phosphorylation by cytokinins. | kinetin stimulated phosphorylation of protein in floated chinese-cabbage leaf discs, but inhibited protein phosphorylation in nuclei+chloroplast extracts from chinese-cabbage or tobacco leaves. kinetin also inhibited protein phosphorylation in isolated tobacco nuclei or nuclei from carrot secondary-phloem tissue. purified chinese-cabbage leaf ribosomes exhibited protein kinase activity which was inhibited by kinetin and zeatin. the ribosome-associated kinase responded to kinetin and zeatin diffe ... | 1972 | 4348261 |
| fine structure of vesicles induced in chloroplasts of chinese cabbage leaves by infection with turnip yellow mosaic virus. | | 1973 | 4584930 |
| leaf ontogeny and virus replication in brassica pekinensis infected with turnip yellow mosaic virus. | | 1972 | 4623503 |
| the sequence of early cytological changes in chinese cabbage leaf cells following systemic infection with turnip yellow mosaic virus. | | 1974 | 4833533 |
| crystallization of turnip yellow mosaic virus in wilted leaves of chinese cabbage by treatment at high temperature. | | 1971 | 5315697 |
| properties of ribulose-1,5-diphosphate carboxylase (carboxydismutase) from chinese cabbage and photosynthetic microorganisms. | ribulose-1,5-diphosphate carboxylase (carboxydismutase) was prepared from chinese cabbage [brassica petsai (parl)] and the k(m) values and molecular weight were determined. these parameters were found to be in good agreement with values reported for this enzyme from other higher plants. investigation of carboxydismutase activity from the photosynthetic micro-organisms chlamydomonas reinhardi (iu 89+), plectonema boryanum (iu 594), and chromatium strain d showed striking similarity to the higher ... | 1968 | 5700019 |
| ribonuclease isozymes in chinese cabbage systemically infected with turnip yellow mosaic virus. | | 1968 | 5723669 |
| virus infection and photosynthesis. 1. increased photophosphorylation by chloroplasts from chinese cabbage infected with turnip yellow mosaic virus. | | 1965 | 5850878 |
| cytological changes induced by turnip yellow mosaic virus in chinese cabbage leaves. | | 1966 | 5938885 |
| length requirements for trna-specific enzymes and cleavage specificity at the 3' end of turnip yellow mosaic virus rna. | this paper describes the minimum length of the turnip yellow mosaic virus (tymv) rna necessary to fulfill the trna-like properties of the viral rna: 50 to 75 nucleotides and 86 nucleotides from the 3' end of tymv rna are sufficient for adenylation and valylation respectively by the escherichia coli system. the size of the trna-like fragments obtained in vitro in the presence of an e. coli, a reticulocyte or a chinese cabbage leaf extract has also been determined. among the major fragments libera ... | 1982 | 6176943 |
| determination of residues of fensulfothion and its metabolites in chinese cabbage, japanese radish , and turnips. | a method was developed using a selective nitrogen-phosphorus detector to determine the combined total residues, as the oxygen analogs, of fensulfothion (o,o-diethyl o-[p-(methylsulfinyl)-phenyl] phosphorothioate) and its 3 metabolites. the procedure was used to determine harvest residues in chinese cabbage, japanese radish, and turnips treated with 2 application rates of fensulfothion. residues found after application of 2 oz active ingredient (ai)/100 lineal ft of row ranged from 0.026 ppm for ... | 1980 | 6447687 |
| preliminary x-ray diffraction analysis of crystals of turnip yellow mosaic virus (tymv). | turnip yellow mosaic virus (tymv) was purified from chinese cabbage and crystallized in a form that permits high resolution structural analysis using x-ray diffraction. the crystals have a hexagonal bipyramidal morphology and often achieve dimensions of 1.0 x 1.0 x 0.5 mm. the crystals appear to be of hexagonal space group p6(2)22 with a=b=525 a, c=315 a, but we cannot strictly rule out the possibility that the space group is p622. they appear different than any crystals of tymv previously repor ... | 1995 | 7716173 |
| evaluation of brassicas in grazing systems for sheep: i. quality of forage and animal performance. | four years of grazing trials were conducted with brassica forages to evaluate their chemical composition and effect on adg of fattening lambs and breeding ewes in late fall. brassicas tested included kales (brassica oleracea l. var. acephala dc), turnips (b. rapa l.), and a chinese cabbage hybrid (b. rapa l. x b. pekinensis [lour.] rupr.). daily gains of lambs varied widely among years (19 to 330 g/d); adg on brassicas were, however, generally higher than on stockpiled kentucky 31 tall fescue (f ... | 1994 | 7928762 |
| evaluation of brassicas in grazing systems for sheep: ii. blood composition and nutrient status. | blood composition of lambs grazing brassicas and stockpiled grass or grass-clover pastures in the fall of 4 yr was monitored to assess possible effects of plant metabolites (e.g., glucosinolates, s-methyl cysteine sulfoxide) on health and performance. serum thyroxine (t4) concentrations in lambs grazing brassicas decreased upon initiation of grazing, with a subsequent recovery, and concentrations were increased by oral dosing with i or i+cuo. serum triiodothyronine (t3) increased gradually with ... | 1994 | 7928763 |
| fermentation and sensory characteristics of kimchi containing potassium chloride as a partial replacement for sodium chloride. | a study was designed to determine the effects of substituting up to 50% of the nacl in kimchi, a fermented chinese cabbage (brassica pekinensis) product containing scallions, garlic, ginger and hot red pepper powder, with kcl. brine water (15% salt) used to soak cabbage contained nacl:kcl ratios of 1:0 (control), 5:1, 2:1 and 1:1 (wt:wt). total acidity and ph of kimchi reached acceptable ranges of 0.4-0.6% (as lactic acid) and 4.4-4.7, respectively, after 13 days of incubation at 13 +/- 1 degree ... | 1994 | 8043352 |
| feed intake and apparent digestibility of hay-supplemented brassica diets for lambs. | animal performance on brassica diets often does not reflect laboratory estimates of the nutritive value of the herbage. hay supplementation of brassica pastures should increase diet dm and fiber intake and dilute anti-quality factors. the optimal hay:brassica ratio needed to achieve desirable animal performance, however, has not been established. effects of changing the hay:brassica ratio on feed and water intake, apparent digestibility of diets, thyroid status, and anemia were examined in five ... | 1994 | 8071189 |
| structure-activity relationships of brassinin in preventing the development of carcinogen-induced mammary lesions in organ culture. | brassinin, a phytoalexin, is found in chinese cabbage. previously, we showed that brassinin significantly inhibited dimethylbenz(a)anthracene (dmba)-induced mammary lesions in organ culture. moreover, it was an effective inhibitor against two stage skin carcinogenesis. in the present study, we synthesized several analogs of brassinin and evaluated their effectiveness in the mouse mammary gland organ culture model. results showed that cyclobrassinin, also a naturally occurring brassinin analog, w ... | 1994 | 8074474 |
| in vitro effect of vegetable and fruit juices on the mutagenicity of 2-amino-3-methylimidazo[4,5-f]quinoline, 2-amino-3,4-dimethylimidazo[4,5-f]quinoline and 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline. | the antimutagenic potencies of the juices of 28 fruits and 34 vegetables commonly consumed in germany were investigated with respect to the mutagenic activities induced by 2-amino-3-methyl[4,5-f]-quinoline (iq), and in part by 2-amino-3,4-dimethylimidazo[4,5-f]quinoline (meiq) or 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (meiqx) in salmonella typhimurium ta98 and ta100. with iq, weak to strong antimutagenic activities were found in 68% of the fruits and 73% of the vegetables that were tested ... | 1994 | 8206443 |
| increased viral yield and symptom severity result from a single amino acid substitution in the turnip yellow mosaic virus movement protein. | turnip yellow mosaic virus is a positive-strand rna virus that produces light green or yellow-green mosaic symptoms in chinese cabbage plants. we have characterized a strain that produces nearly uniform yellow-green chlorosis in systemically infected chinese cabbage leaves. the increased symptom severity is due to the single nucleotide substitution u1888-->c, which results in a tyrosine to histidine substitution in the movement protein encoded by orf-69. coding by the overlapping orf-206 is not ... | 1993 | 8324245 |
| electrotransfection of turnip yellow mosaic virus rna into brassica leaf protoplasts and detection of viral rna products with a non-radioactive probe. | we describe here a convenient and efficient system for studying turnip yellow mosaic virus (tymv) replication in leaf protoplasts. inoculation of rapeseed (brassica napus) or chinese cabbage (b. sinensis) protoplasts was achieved via electroporation, and sensitive detection of viral rna products was performed by northern blot analyses using a non-radioactive digoxigenin-labelled cdna probe. virus replication was detected when 1.5 x 10(6) rapeseed protoplasts were inoculated with 20 ng of tymv rn ... | 1993 | 8376966 |
| characterization of a cdna encoding cysteine proteinase inhibitor from chinese cabbage (brassica campestris l. ssp. pekinensis) flower buds. | a cdna encoding a new phytocystatin isotype named bcpi-1 was isolated from a cdna library of chinese cabbage flower buds. the bcpi-1 clone encodes 199 amino acids resulting in a protein much larger than other known phytocystatins. bcpi-1 has an unusually long c-terminus. a bcpi-1 fusion protein expressed in escherichia coli strongly inhibits the enzymatic activity of papain, a cysteine proteinase. genomic southern blot analysis revealed that the bcpi gene is a member of a small multi-gene family ... | 1996 | 8616262 |
| promoter sequences of two homologous pectin esterase genes from chinese cabbage (brassica campestris l. ssp. pekinensis) and pollen-specific expression of the gus gene driven by a promoter in tobacco plants. | the promoter regions of two genomic clones, gban215-6 and gban215-12 from chinese cabbage (brassica campestris l. ssp. pekinensis), were sequenced. the nucleotide sequences of their promoter regions were compared with that of the bp19 pollen-specific gene of brassca napus. high nucleotide sequence homologies were observed among these three genes in the region between 210 bp upstream and the putative transcription start site. a sequence motif tgtggtg, which is similar to that of the pb core motif ... | 1997 | 9085260 |
| turnip yellow mosaic virus rna-dependent rna polymerase: initiation of minus strand synthesis in vitro. | an rna-dependent rna polymerase (rdrp) activity was detergent-solubilized from the chloroplast membranes of chinese cabbage leaves infected with turnip yellow mosaic virus (tymv). the template-dependent, micrococcal nuclease-treated activity synthesized full-length minus strands from tymv rna and 3'-fragments as short as a 28-nucleotide-long rna comprising the amino acid acceptor stem of the 3'-trna-like structure (tls). minus strands were shown to arise by de novo initiation with the insertion ... | 1997 | 9217066 |
| a functional role for the conserved protonatable hairpins in the 5' untranslated region of turnip yellow mosaic virus rna. | the 5' untranslated region (utr) of the rna of several tymoviruses contains conserved hairpins with protonatable internal loops, consisting of c-c and c-a mismatches (k. hellendoorn, p. j. a. michiels, r. buitenhuis, and c. w. a. pleij, nucleic acids res. 24, 4910-4917, 1996). here, we present a functional analysis of the 5' utr of turnip yellow mosaic virus (tymv) rna, which contains two protonatable hairpins with nearly identical internal loops. mutations were introduced in an infectious cdna ... | 1997 | 9343237 |
| lifestyle and anti-helicobacter pylori immunoglobulin g antibody among outpatients. | since eradication of helicobacter pylori (h. pylori) is thought to be a preventive measure against stomach cancer, several studies have examined factors associated with the infection. this paper reports the association of the infection with lifestyle factors observed in a hospital-based case-control study. cases were 140 anti-h. pylori igg antibody-positive outpatients (75 males and 65 females). controls were 52 antibody-negative outpatients (22 males and 30 females). both groups had undergone g ... | 1997 | 9439678 |
| turnip yellow mosaic virus isolates with experimentally produced recombinant virion proteins. | the consensus secondary structure of the virion proteins (vps) of tymoviruses was predicted from their amino acid sequences using a combination of computer methods: profile alignment, hydrophobicity moment and 'predictprotein'. all methods predicted that they were eight-stranded anti-parallel beta-barrels with two alpha-helical regions. the predicted structure was used to design recombinants of turnip yellow mosaic virus (tymv) in which selected parts of its vp were replaced with homologous regi ... | 1998 | 9472626 |
| virion protein sequence variation among australian isolates of turnip yellow mosaic tymovirus. | the virion protein genes, and 3' untranslated regions, of six variants of turnip yellow mosaic tymovirus (tymv) that produced different symptoms in their native host cardamine robusta and in chinese cabbage plants, have been sequenced. the sequences have been compared with each other, and with the same region of the pbl-16 clone of the blue lake isolate of tymv. the sequences of the virion protein genes differed by a mean of 1.89% (range 0-2.82%), and the encoded proteins by a mean of 1.71% (ran ... | 1998 | 9505977 |
| pollen ablation of transgenic tobacco plants by expression of the diphtheria toxin a-chain gene under the control of a putative pectin esterase promoter from chinese cabbage. | we previously showed that a 383 bp (-274 to approximately +109) promoter of a pollen-specific gene, gban215-6, had a property of a late gene in pollen development in transgenic tobacco plants. it drove gus gene expression from uninucleate microspores to pollen tube growth of trinucleated cells. to more precisely characterize the specificity of the promoter, we placed the diphtheria toxin a-chain (dtx-a) coding region under the control of the gban215-6 promoter. transgenic tobacco plants containi ... | 1998 | 9666468 |
| diet and brain cancer in adults: a case-control study in northeast china. | a hospital-based case-control study was conducted in the heilongjiang province of northeast china between may 1993 and may 1995. a total of 129 histologically confirmed brain cancer cases (73 gliomas and 56 meningiomas) and 258 matched controls were interviewed in 6 major hospitals to examine the influence of dietary factors in developing brain cancer. information was obtained about frequency of consumption of 57 food items. odds ratios (ors) were obtained from conditional logistic regression, i ... | 1999 | 10077146 |
| cloning and expression of a new isotype of the peroxiredoxin gene of chinese cabbage and its comparison to 2cys-peroxiredoxin isolated from the same plant. | a cdna encoding a newly identified isotype of peroxiredoxin (prx) was isolated from a chinese cabbage flower bud cdna library and designated cprxii. database searches using the predicted cprxii amino acid sequence revealed no substantial homology to other proteins with the exception of the yeast type ii prx with which cprxii shares 27.8% sequence identity. recombinant cprxii expressed in escherichia coli was able to protect glutamine synthetase from inactivation in a metal-catalyzed oxidation sy ... | 1999 | 10329461 |
| [effect of green vegetable on digestive tract absorption of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans in rats]. | the effect green vegetable on fecal excretion of polychlorinated dibenzo-p-dioxin (pcdd) congeners and polychlorinated dibenzofuran (pcdf) congeners was examined in male rats. the rats were administered 10% vegetable diets or a basal diet containing 0.2 ml of the causal rice-bran oil of yusho that had occurred in the southwest part of japan in 1968 and kept on the same diet for five days. the fecal excretion of 2,3,7,8-t4cdd and 2,3,4,7,8-p5cdf in the group fed with komatsuna, mitsuba, spinach a ... | 1999 | 10396873 |
| molecular cloning, expression, and functional characterization of a 2cys-peroxiredoxin in chinese cabbage. | a cdna (c2c-prx) corresponding to a 2cys-peroxiredoxin (2cys-prx) was isolated from a leaf cdna library of chinese cabbage. the predicted amino acid sequence of c2c-prx has 2 conserved cysteines and several peptide domains present in most of the 2cys-prx subfamily members. it shows the highest sequence homology to the 2cys-prx enzymes of spinach (88%) and arabidopsis (86%). southern analysis using the cdna insert of c2c-prx revealed that it consists of a small multigene family in chinese cabbage ... | 1999 | 10487217 |
| aiia, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of erwinia carotovora. | n-acylhomoserine lactones, known as autoinducers (ais), are widely conserved signal molecules present in quorum-sensing systems of many gram-negative bacteria. ais are involved in the regulation of diverse biological functions, including expression of pathogenic genes in the plant pathogens pseudomonas solanacearum, several erwinia species, and the human pathogen pseudomonas aeruginosa. a bacterial isolate, bacillus sp. 240b1, is capable of enzymatic inactivation of ais. the gene (aiia) for ai i ... | 2000 | 10716724 |
| [choice of plant light status for space greenhouse: results of ground-based experience]. | to decide on the light status of plants in space greenhouse, a theoretical study was undertaken to correlate specific productivity of space greenhouse with illumination characteristics including vertical par flux density (i), photoperiod (tau), and crop leaf index (l). it was demonstrated that in pace with i the daily productivity per a volume unit tended to monotonously approach maximum at i = ip, whereas the greenhouse energy efficiency me peaked at i = ie, ik < ie < ip, where ik is a compensa ... | 2000 | 10732194 |
| synthesis and biological activities of 4-chloroindole-3-acetic acid and its esters. | 4-chloroindole-3-acetic acid (4-cl-iaa) and its esters were synthesized from 2-chloro-6-nitrotoluene as the starting material. the biological activities of 4-ci-iaa and its esters were determined by four bioassays. except for the tert-butyl ester, 4-cl-iaa and its esters had stronger elongation activity toward avena coleoptiles than had indole-3-acetic acid. the biological activities of the methyl, ethyl and allyl esters were as strong as the activity of the free acid. all the esters, except for ... | 2000 | 10830497 |
| [chemoprevention of 2-amino-1-methyl-6-phenylimidazo[4,5-b] pyridine-induced carcinogen-dna adducts by chinese cabbage in rats]. | to examine the preventive effect of chinese cabbage (brassica chinensis) against colon carcinogen 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (phip)-induced phip-dna adduct formation in rats and its possible mechanism. | 1998 | 10920929 |
| chromatographic separation of a small subunit (psbw/psay) and its assignment to photosystem i reaction center. | by using a hydroxyapatite column, the five major photosystem i (psi) subunits (psaa,-b,-c,-d,-e) solubilized by sodium dodecyl sulfate (sds) were fractionated from a spinach psi reaction center preparation. another small (5-6 kda) polypeptide was also separated, and purified to homogeneity. mass spectroscopy yielded its molecular weight to be 5942 +/- 10. this polypeptide had an n-terminal sequence homologous to those of previously reported 5-kda subunits from spinach and wheat and a 6.1-kda sub ... | 2000 | 10924904 |
| seasonal abundance of the parasitoid complex associated with the diamondback moth, plutella xylostella (lepidoptera: plutellidae) in hangzhou, china. | an investigation of insect parasitoids of the diamondback moth, plutella xylostella (linnaeus), in brassica vegetable crops in the suburbs of hangzhou was conducted during five periods from 1989 to 1997. eight species of primary parasitoids were recorded: trichogramma chilonis ishii, cotesia plutellae kurdjumov, microplitis sp., oomyzus sokolowskii kurdjumov, diadromus collaris (gravenhorst), itoplectis naranyae (ashmead), exochus sp. and brachymeria excarinata gahan. seven species of hyperparas ... | 2000 | 10996863 |
| fistupyrone, a novel inhibitor of the infection of chinese cabbage by alternaria brassicicola, from streptomyces sp. tp-a0569. | a new microbial metabolite, designated fistupyrone, was isolated from the culture broth of a plant-associated streptomyces sp. tp-a0569. fistupyrone inhibited the in vivo infection of the seedlings of chinese cabbage by alternaria brassicicola tp-f0423, the cause of alternaria leaf spot, without any in vitro fungicidal activity. | 2000 | 11132956 |
| flavonoid (myricetin, quercetin, kaempferol, luteolin, and apigenin) content of edible tropical plants. | studies were conducted on the flavonoids (myricetin, quercetin, kaempferol, luteolin, and apigenin) contents of 62 edible tropical plants. the highest total flavonoids content was in onion leaves (1497.5 mg/kg quercetin, 391.0 mg/kg luteolin, and 832.0 mg/kg kaempferol), followed by semambu leaves (2041.0 mg/kg), bird chili (1663.0 mg/kg), black tea (1491.0 mg/kg), papaya shoots (1264.0 mg/kg), and guava (1128.5 mg/kg). the major flavonoid in these plant extracts is quercetin, followed by myrice ... | 2001 | 11410016 |
| the superiority of organically cultivated vegetables to general ones regarding antimutagenic activities. | we found organically cultivated (oc) vegetables, using a water-soluble chitosan as a soil improvement agent and leaf surface spray, had much longer shelf life and better taste than that of generally cultivated (gc) vegetables. the purpose of this study is to determine the relative antimutagenic activity between oc and gc vegetables. eleven oc vegetables were harvested in march and april in 1999 and 2000, and gc ones were supplied as a control from nearby farms on the same date. the former vegeta ... | 2001 | 11551483 |
| mode of antagonism of brevibacillus brevis against botrytis cinerea in vitro. | to assess the activity of brevibacillus brevis (formerly bacillus brevis) nagano and the antibiotic it produces, gramicidin s, against the plant pathogen botrytis cinerea. | 2001 | 11576302 |
| interaction of turnip yellow mosaic virus val-rna with eukaryotic elongation factor ef-1 [alpha]. search for a function. | the 3'-terminal trna-like structure in turnip yellow mosaic virus (tymv) rna can be adenylated by trna nucleotidyltransferase and subsequently aminoacylated by valyl-trna synthetase. here we present evidence that tymv val-rna can form a stable complex with eukaryotic wheat germ elongation factor ef-1alpha and gtp: the val-rna is protected by ef-1alpha.. gtp against digestion by rnase a. by affinity chromatography of tymv val-rna fragments on immobilized ef-1alpha . gtp, it has been established t ... | 1986 | 11681331 |
| chemoprevention of 2-amino-1-methyl-6-phenyli-midazo 4,5-b pyridine-induced carcinogen-dna adducts by chinese cabbage in rats. | aim:the food-borne carcinogen 2-amino-1-methyl-6-phenylimidazo 4,5-b pyridine (phip) induces colon and mammary gland tumors in rats and has been implicated in the etiology of human colorectal cancer.this study was conducted to examine the potentially preventive effect of chinese cabbage (brassica chinensis),a brassica vegetable most commonly consumed in china, against this carcinogen-induced dna adduct formation in rats and its possible mechanisms.methods:sprague-dawley rats were maintained for ... | 1999 | 11819413 |
| a chinese cabbage cdna with high sequence identity to phospholipid hydroperoxide glutathione peroxidases encodes a novel isoform of thioredoxin-dependent peroxidase. | a cdna, phcc-tpx, specifying a protein highly homologous to known phospholipid hydroperoxide glutathione peroxidases was isolated from a chinese cabbage cdna library. phcc-tpx encodes a preprotein of 232 amino acids containing a putative n-terminal chloroplast targeting sequence and three conserved cys residues (cys(107), cys(136), and cys(155)). the mature form of enzyme without the signal peptide was expressed in escherichia coli, and the recombinant protein was found to utilize thioredoxin (t ... | 2002 | 11823460 |
| the symbiosis of bacillus subtilis l-forms with chinese cabbage seedlings inhibits conidial germination of botrytis cinerea. | to establish whether germination of botrytis cinerea was affected by the symbiosis of bacillus subtilis l-form bacteria with chinese cabbage. | 2002 | 11849491 |
| use of waste chinese cabbage as a substrate for yeast biomass production. | the possibility of using waste chinese cabbage (brassica campestris) as a substrate for microbial biomass production was investigated. the juice from waste chinese cabbage contains relatively high amounts of reducing sugars suitable for yeast culture. the cell mass and protein content of four species of yeast, candida utilis, pichia stipitis, kluyveromyces marxianus, and saccharomyces cerevisiae, were determined when cultured in juice extracted from cabbage waste. compared to ym broth containing ... | 2002 | 12094802 |
| characterization of a stamen-specific cdna encoding a novel plant defensin in chinese cabbage. | we isolated a stamen-specific cdna, bsd1 (brassica stamen specific plant defensin 1) that encodes a novel plant defensin peptide in chinese cabbage (brassica campestris l. ssp. pekinensis). plant defensins are antimicrobial peptides containing eight highly conserved cysteine residues linked by disulfide bridges. in bsd1, the eight cysteine residues and a glutamate residue at position 29 are conserved whereas other amino acid residues of the plant defensins consensus sequence are substituted. bsd ... | 2002 | 12139009 |
| ban103, a pollen-preferential gene, from chinese cabbage and its promoter activity. | we isolated and characterized a pollen-preferential gene, ban103, from chinese cabbage and analyzed the activity of its promoter. the ban103 cdna and genomic clone that contained the full-length gene were sequenced. the ban103 gene is a single copy in the chinese cabbage genome, and divided into three exons by two introns. the deduced sequence of 68 amino acids showed a homology with the brassica oleracea pollen coat protein, as well as several cold-induced proteins. ban103 transcription was res ... | 2002 | 12243345 |
| structural variation and functional importance of a d-loop-t-loop interaction in valine-accepting trna-like structures of plant viral rnas. | valine-accepting trna-like structures (tlss) are found at the 3' ends of the genomic rnas of most plant viruses belonging to the genera tymovirus, furovirus, pomovirus and pecluvirus, and of one tobamovirus species. sequence alignment of these tlss suggests the existence of a tertiary d-loop-t-loop interaction consisting of 2 bp, analogous to those in the elbow region of canonical trnas. the conserved g(18).psi(55) pair of regular trnas is found to covary in these tlss between g.u (possibly also ... | 2002 | 12364602 |
| assessing copper thresholds for phytotoxicity and potential dietary toxicity in selected vegetable crops. | copper pollution in soils is widespread, and its accumulation in crop products could pose a risk on human health. in this paper, bioavailability of added copper (cu) and critical cu concentrations in a vegetable garden soil was evaluated for chinese cabbage (brassica chinensis l.), pakchoi (brassica chinensis l.), and celery (apiumg graveolens l. var. dulce dc) based on human dietary toxicity. the availability of added cu in the soil decreased with incubation time, and had minimal change after 1 ... | 2002 | 12403270 |
| isolation of pathogen-induced chinese cabbage genes by subtractive hybridization employing selective adaptor ligation. | we have developed a subtractive cloning method in which target sequences are effectively enriched by selective adaptor ligation and pcr after hybridization. in this method both tester and driver dnas are digested with rsai, ligated with the linker dna containing a kpni recognition site, and amplified by pcr. the tester dna samples are divided into two aliquots, each digested with either rsai or kpni. the two dna samples are then combined and hybridized with an excess of the driver dna retaining ... | 2002 | 12445806 |
| [prokaryote expression and western analysis of bcplh gene of chinese cabbage]. | bcplh gene preferentially expressed in folding leaf of chinese cabbage contains dsrna-binding domains. the cdna of bcplh gene was cloned into a his-fusion expression vector pet-28a (+) and was induced to express in e. coli strain bl21 (de3). then, the specific protein was partially purified and the rabbit was immunized to prepare the anti-serum. meanwhile bcplh cdna was cloned into the pmal-c2 containing the solubizing partner, and then the soluble protein generated. it was demonstrated from wes ... | 2000 | 12549068 |
| an outbreak of infections with a new salmonella phage type linked to a symptomatic food handler. | in december 2001, the south australian communicable disease control branch investigated an outbreak of gastrointestinal illness linked to a korean style restaurant in metropolitan adelaide. twenty-eight people were identified as having experienced gastrointestinal symptoms subsequent to dining at the restaurant between 9 and 12 december 2001. a case-control study implicated mango pudding dessert (or 16.67 95% ci 2.03-177.04) and plain chicken (or 10.67 95% ci 1.04-264.32). nineteen cases and one ... | 2002 | 12549524 |
| botanicals in cancer chemoprevention. | botanicals have been used for the treatment of various human diseases throughout history. in addition, botanicals play a role in disease prevention. for example, epidemiologic studies have suggested that a reduced risk of cancer is associated with high consumption of vegetables and fruits. thus, the cancer chemopreventive potential of naturally occurring phytochemicals is of great interest. in this review, we discuss the cancer chemopreventive activity of cruciferous vegetables such as cabbage a ... | 2002 | 12549763 |
| [type iii secretion system in se9r can recognize and secret harpin in erwinia amylovora]. | bacterial pathogens of both animals and plants use type iii secretion machines to secret virulence proteins. the signal that leads to tye type iii secretion is encoded as stem loop structure in the messenger rna. harpin, produced by erwinia anylovora is secreted via type iii secretion. erwinia carotovora se9r and erwinia amylovora belong to the same genus and they use type iii secretion machines to secret virulence proteins. we used pcr to amplify hrpn from pcpp430 which harbors hrpn gene cluste ... | 2002 | 12557554 |
| comparative physiological responses in chinese cabbage induced by herbivory and fungal infection. | fungal infection of chinese cabbage leaves by alternaria brassicae has earlier been shown to have detrimental effects on larval development of the chrysomelid beetle phaedon cochleariae. furthermore, adults of this leaf beetle avoid fungus-infected chinese cabbage leaves for oviposition and feeding. however, herbivory had no impact on fungal growth. in this study, we investigated physiological responses of the host plant to herbivore attack and fungal infection in order to elucidate the mechanis ... | 2002 | 12564792 |
| indirect interactions between a phytopathogenic and an entomopathogenic fungus. | slow growth in herbivores may lead to higher mortality, due to prolonged exposure to natural enemies. a number of studies has tested the 'slow-growth, high-mortality' hypothesis using predators or parasitoids as natural enemies of the herbivore, but the possible role of pathogens is poorly documented. we provide evidence that phytopathogenic infection of a plant enhances the susceptibility of herbivore larvae to a generalist entomopathogen. larvae of the mustard leaf beetle phaedon cochleariae g ... | 2003 | 12590299 |
| stimulation by caffeic acid, coumalic acid, and corilagin of the germination of resting spores of the clubroot pathogen plasmodiophora brassicae. | some chemicals were examined for their effects on the germination of resting spores of the clubroot pathogen plasmodiophora brassicae, and on the control of clubroots in chinese cabbage. caffeic acid, coumalic acid, and corilagin stimulated the germination of plasmodiophora spores and prevented the formation of clubroots in chinese cabbage. clubroot might be controlled by agents with germination-stimulating effects. | 2003 | 12619690 |
| effects of cyazofamid against plasmodiophora brassicae woronin on chinese cabbage. | cyazofamid (4-chloro-2-cyano-n,n-dimethyl-5-p-tolylimidazole-1-sulfonamide) is a novel fungicide with high levels of activity against oomycetes fungi and plasmodiophora brassicae woronin. the effects of cyazofamid were investigated against p. brassicae, the causal agent of clubroot disease in chinese cabbage. cyazofamid at 0.3 mg litre(-1) inhibited resting spore germination of this pathogen by about 80%. cyazofamid at 3-10 mg litre(-1) exhibited fungicidal activity to resting spores of p. brass ... | 2003 | 12639045 |
| nitrate leaching in an andisol treated with different types of fertilizers. | nitrate (no3) leaching was studied in an andisol treated with four n fertilizers (sc: swine compost, cu: coated urea, an: ammonium n, or nf: no fertilizer) for 7 years. sweet corn (zea mays l.) was grown in summer, followed by chinese cabbage (brassica rapa l. var. amplexicaulis) or cabbage (brassica oleracea l. var. capitata) in autumn each year. in chemical fertilizer plots treated with an or cu, no(3)-n concentrations in soil water at 1-m depth increased markedly in the summer of the second y ... | 2003 | 12685774 |
| characterization of the xanthomonas axonopodis pv. glycines hrp pathogenicity island. | we sequenced an approximately 29-kb region from xanthomonas axonopodis pv. glycines that contained the hrp type iii secretion system, and we characterized the genes in this region by tn3-gus mutagenesis and gene expression analyses. from the region, hrp (hypersensitive response and pathogenicity) and hrc (hrp and conserved) genes, which encode type iii secretion systems, and hpa (hrp-associated) genes were identified. the characteristics of the region, such as the presence of many virulence gene ... | 2003 | 12730176 |
| peh production, flagellum synthesis, and virulence reduced in erwinia carotovora subsp. carotovora by mutation in a homologue of cytr. | erwinia carotovora subsp. carotovora is a causal agent of soft-rot diseases in a wide variety of plants. here, we have isolated a new regulatory factor involved in the virulence of e. carotovora subsp. carotovora by in vivo insertional mutagenesis using a transposon tn5. the gene was homologous to cytr encoding a transcriptional repressor of nucleoside uptake and catabolism genes in escherichia coli, salmonella typhimurium, and vibrio cholerae. phenotypic characterization of a nonpolar deletion ... | 2003 | 12744509 |
| growth conditions, elemental accumulation and induced physiological changes in chinese cabbage. | soils contaminated with low levels of heavy metals and other trace elements are now frequently used for vegetable growing. in this situation, heavy metals and trace elements from these polluted soils may accumulate in the agricultural plants being grown in them and thereby enter the human food chain. the objectives of this study are to elucidate the effects of growth conditions, manipulated by the crop covers, on the phytoaccumulation of elements, and to investigate the conceivable influences of ... | 2003 | 12781236 |
| characterization of salicylic acid-induced genes in chinese cabbage. | salicylic acid is a messenger molecule in the activation of defense responses in plants. in this study, we isolated four cdna clones representing salicylic acid-induced genes in chinese cabbage (brassica rapa subsp. pekinensis) by subtractive hybridization. of the four clones, the bc5-2 clone encodes a putative glucosyltransferase protein. the bc5-3 clone is highly similar to an arabidopsis gene encoding a putative metal-binding farnesylated protein. the bc6-1 clone is a chitinase gene with simi ... | 2003 | 12835914 |
| molecular detection and beta-glucuronidase expression of gus-marked bacillus subtilis l-form bacteria in developing chinese cabbage seedlings. | to detect l-form bacteria in developing chinese cabbage seedlings. | 2003 | 12859751 |
| urinary isothiocyanate levels, brassica, and human breast cancer. | brassica vegetable consumption (e.g., chinese cabbage) provides isothiocyanates (itc) and other glucosinolate derivatives capable of inducing phase ii enzymes [e.g., glutathione s-transferases (gstm1, gstt1, and gstp1) and nadph quinine oxidoreductase] and apoptosis, altering steroid hormone metabolism, regulating estrogen receptor response, and stabilizing cellular proliferation. asian populations consuming large amounts of brassica have a lower breast cancer incidence compared with western pop ... | 2003 | 12873994 |
| [change in incidence of parasite eggs and larvae from vegetable in the markets of taegu] | a survey on vegetables, which consisted of lettuce (lactuca sativa), young radish (raphanus sapivus), and chinese cabbage (brassica pekinensis) collected from 6 markets in the taegu city, was conducted for the discovery of helminth eggs and larvae, from july 1982 to june 1983. the results were compared with the data obtained from vegetables collected at the same markets by lee (1969) and choi and lee (1972). both sides of vegetable leaves were washed carefully with a hard brush, and the species ... | 1983 | 12902676 |
| incidence of parasites found on vegetables collected from markets and vegetable gardens in taegu area. | a parasitic survey on vegetables collected from markets and vegetable gardens in taegu area was conducted for the discovery of human parasitic eggs and larvae. three species of vegetable, lettuce (lactuca sativa), young radish(raphanus sapivus) and chinese cabbage (brassica pekinensis) were selected. all vegetable leaves were washed with a hard brush, and then species of parasites and the approximate mean number of parasitic eggs per 200 grams of vegetable leaves were investigated. when vegetabl ... | 1972 | 12913510 |
| induction of male sterile cabbage using a tapetum-specific promoter from brassica campestris l. ssp. pekinensis. | the anther (tapetum)-specific gene bca9 was isolated from chinese cabbage, brassica campestris l. ssp. pekinensis cv. jangwon, using the arabidopsis tapetum-specific a9 gene as a probe. the dna and amino acid sequences of the coding region of the bca9 gene showed high homology with a9 genes from arabidopsis and b. napus. however, the dna sequences of the 5' noncoding (promoter) region were different, except for the sequence from -281 to -89. to test the specific activity of this promoter, a plan ... | 2003 | 12920565 |
| [chemical composition of volatile oil from chromolaena odorata and its effect on plant, fungi and insect growth]. | the volatile oil from c. odorata had a significant effect on the growth of plants, fungi and insects. the result showed that the seedling growth of five test plants was obviously inhibited, in the order of ryegrass > chinese cabbage > radish > snap been > rice. the inhibitory effect of the oil at its middle concentration (800 mg.l-1) for pyricularia grisea was the strongest, the next was to phytophthora nicotianae, and the weakest was to fusarium axysporum. the inhibitory percentage was 61.40%, ... | 2003 | 12924132 |
| characterization of a salicylic acid- and pathogen-induced lipase-like gene in chinese cabbage. | a cdna clone for a salicylic acid-induced gene in chinese cabbage (brassica rapa subsp. pekinensis) was isolated and characterized. the cabbage gene, designated br-sil1 (for brassica rapa salicylate-induced lipase-like 1 gene), encodes a putative lipase that has the family ii lipase motif gdsxxdxg around the active site serine. a database search showed that plant genomes have a large number of genes that contain the family ii lipase motif. the lipase-like proteins include a myrosinase-associated ... | 2003 | 14536025 |
| environmental concerns related to high thallium levels in soils and thallium uptake by plants in southwest guizhou, china. | thallium (tl) contamination in soils poses a significant threat to human health due to the high toxicity of tl and its ready assimilation by crops. this study is focused on high concentrations of tl in soils in the lanmuchang area of southwest guizhou, china, which is related to natural processes of tl-rich sulfide mineralization. thallium contents range from 40 to 124 mg/kg in soils originating from the mining area, from 20 to 28 mg/kg in slope wash materials, from 14 to 62 mg/kg in alluvial de ... | 2004 | 14654287 |