| growth of primary hippocampal neuronal tissue on an aragonite crystalline biomatrix. | tissue-like structures of hippocampal neurons were established in a crystalline three-dimensional (3d) aragonite biomatrix obtained from the exoskeleton of the coral porites lutea. cultures were maintained in vitro for up to 5 weeks. cell viability and regeneration of neuronal properties were studied by immunocytochemical methods, light microscopy image analysis techniques, and scanning electron microscopy. some portions of the cell population acquired the morphological characteristics of hippoc ... | 2005 | 15871670 |
| aragonite crystalline biomatrices support astrocytic tissue formation in vitro and in vivo. | astrocytes play a pivotal role in the development and function of the central nervous system by regulating synaptic activity and supporting and guiding growing axons. it is therefore a central therapeutic and scientific challenge to develop means to control astrocytic survival and growth. we cultured primary hippocampal astrocytes on a crystalline three-dimensional (3d) aragonite biomatrix prepared from the exoskeleton of the coral porites lutea. such culturing led to the formation of astrocytic ... | 2006 | 16889507 |
| biomaterial effects in articular cartilage tissue engineering using polyglycolic acid, a novel marine origin biomaterial, igf-i, and tgf-beta 1. | bovine articular chondrocytes were seeded on either polyglycolic acid (pga) non-woven mesh scaffolds or a biomatrix from the species porites lutea (por). these constructs were cultured for 6 weeks in the presence of insulin-like growth factor (igf)-i (10 ng/ml or 100 ng/ml) or transforming growth factor (tgf)-beta 1 (5 ng/ml or 30 ng/ml) to determine the in-vitro articular cartilage regeneration capacity of each. histology, deoxyribonucleic acid content, collagen i and ii (immunohistochemistry a ... | 2009 | 19239068 |
| culture and differentiation of osteoblasts on coral scaffold from human bone marrow mesenchymal stem cells. | in this paper we describe an approach that aims to provide fundamental information towards a scientific, biomechanical basis for the use of natural coral scaffolds to initiate mesenchymal stem cells into osteogenic differentiation for transplant purposes. biomaterial, such as corals, is an osteoconductive material that can be used to home human derived stem cells for clinical regenerative purposes. in bone transplantation, the use of biomaterials may be a solution to bypass two main critical obs ... | 2010 | 20703817 |
| bacteria associated with an encrusting sponge (terpios hoshinota) and the corals partially covered by the sponge. | terpios hoshinota, a dark encrusting sponge, is known to be a competitor for space in coral reef environments, and facilitates the death of corals. although numerous cyanobacteria have been detected in the sponge, little is known of the sponge-associated bacterial community. this study examined the sponge-associated bacterial community and the difference between the bacterial communities in the sponge and the coral partially covered by the sponge by analysis of 16s rrna gene sequences of samples ... | 2011 | 21265978 |
| the shifts of diazotrophic communities in spring and summer associated with coral galaxea astreata, pavona decussata, and porites lutea. | the coral holobiont often resides in oligotrophic waters; both coral cells and their symbiotic dinoflagellates possess ammonium assimilation enzymes and potentially benefit from the nitrogen fixation of coral-associated diazotrophs. however, the seasonal dynamics of coral-associated diazotrophs are not well characterized. here, the seasonal variations of diazotrophic communities associated with three corals, galaxea astreata, pavona decussata, and porites lutea, were studied using nifh gene ampl ... | 2016 | 27920768 |
| highly heterogeneous bacterial communities associated with the south china sea reef corals porites lutea, galaxea fascicularis and acropora millepora. | coral harbor diverse and specific bacteria play significant roles in coral holobiont function. bacteria associated with three of the common and phylogenetically divergent reef-building corals in the south china sea, porites lutea, galaxea fascicularis and acropora millepora, were investigated using 454 barcoded-pyrosequencing. three colonies of each species were sampled, and 16s rrna gene libraries were constructed individually. analysis of pyrosequencing libraries showed that bacterial communit ... | 2013 | 23940737 |
| detection of polyketide synthase and nonribosomal peptide synthetase biosynthetic genes from antimicrobial coral-associated actinomycetes. | the diversity and properties of actinobacteria, predominant residents in coral holobionts, have been rarely documented. in this study, we aimed to explore the species diversity, antimicrobial activities and biosynthetic potential of culturable actinomycetes within the tissues of the scleractinian corals porites lutea, galaxea fascicularis and acropora millepora from the south china sea. a total of 70 strains representing 13 families and 15 genera of actinobacteria were isolated. the antimicrobia ... | 2014 | 25190333 |
| bacterial communities associated with porites white patch syndrome (pwps) on three western indian ocean (wio) coral reefs. | the scleractinian coral porites lutea, an important reef-building coral on western indian ocean reefs (wio), is affected by a newly-reported white syndrome (ws) the porites white patch syndrome (pwps). histopathology and culture-independent molecular techniques were used to characterise the microbial communities associated with this emerging disease. microscopy showed extensive tissue fragmentation generally associated with ovoid basophilic bodies resembling bacterial aggregates. results of 16s ... | 2013 | 24391819 |
| identification of a bacterial pathogen associated with porites white patch syndrome in the western indian ocean. | porites white patch syndrome (pwps) is a coral disease recently described in the western indian ocean. this study aimed to isolate and identify potential pathogens associated with pwps utilizing both culture and nonculture screening techniques and inoculation trials. a total of 14 bacterial strains (those dominant in disease lesions, absent or rare in healthy tissues and considered potential pathogens in a previous study) were cultured and used to experimentally inoculate otherwise healthy indiv ... | 2015 | 26193772 |
| differences in physiological response to increased seawater temperature in nearshore and offshore corals in northern vietnam. | effects of elevated seawater temperature show high spatial heterogeneity and variation within and among coral species. the objective of this study was to investigate how two coral species, porites lutea and galaxea fascicularis, from two high latitude reefs differently exposed to chronic disturbance, respond to elevated seawater temperatures. corals were collected from reefs nearshore (i.e. subjected to high sediment load, higher chlorophyll α concentrations, turbidity etc.) and offshore (i.e. l ... | 2011 | 21324522 |
| orstomella yaliuensis n. sp., a xarifiid copepod (crustacea) parasitic in the polyps of hump coral porites lutea milne edwards & haime off taiwan. | a new species of xarifiid copepod, orstomella yaliuensis n. sp., is described. it was found living in the polyps of a scleractinian coral, porites lutea milne edwards & haime, collected off yaliu in the northern part of taiwan. the new species can be distinguished from its two congeners, o. faviae humes & ho, 1968 and o. lobophylliae humes & ho, 1968, by the presence of five (rather than six) segments on the antennule, a spine only (rather than a seta and a spine) at the tip of the antenna, a un ... | 2009 | 19633927 |