| metal resistance in acidocella strains and plasmid-mediated transfer of this characteristic to acidiphilium multivorum and escherichia coli. | acidophilic heterotrophic strain gs19h of the genus acidocella exhibited extremely high resistance to cdso4 and znso4, with a mic of 1 m for each. the respective mics for an acidocella aminolytica strain were 400 and 600 mm. the mics of niso4 for the above strains were 200 and 175 mm, respectively. these strains were also resistant to cuso4, the mics being 20 and 40 mm, respectively. an acidocella facilis strain showed resistance only to znso4, with a mic of 150 mm. the metal salts, in general, ... | 1997 | 9361438 |
| plasmid curing from an acidophilic bacterium of the genus acidocella. | preservation of the acidophilic heterotroph, acidocella sp. strain gs19h, at 4 degrees c in stab culture eliminated all indigenous plasmids from this bacterium. growth at 42 degrees c initially caused changes in the plasmid profile followed by total elimination of plasmids after 10 cycles of growth. concomitant to this loss of all plasmids, the cured derivatives became sensitive to cdso(4) and znso(4), and the mic value of the salts dropped from 1 m for each in the case of parental strain to 2 m ... | 2000 | 10675596 |
| isolation and phylogenetic characterization of acidophilic microorganisms indigenous to acidic drainage waters at an abandoned norwegian copper mine. | the biodiversity of culturable acidophilic microbes in three acidic (ph 2.7-3.7), metal-rich waters at an abandoned subarctic copper mine in central norway was assessed. acidophilic bacteria were isolated by plating on selective solid media, and dominant isolates were identified from their physiological characteristics and 16s rrna gene sequences. the dominant iron-oxidizing acidophile in all three waters was an acidithiobacillus ferrooxidans-like eubacterium, which shared 98% 16s rdna identity ... | 2001 | 11722543 |
| naphthalene-utilizing and mercury-resistant bacteria isolated from an acidic environment. | soil samples were taken from areas of low ph (2.5-3.5) surrounding an outdoor coal storage pile. these samples were added to medium with naphthalene as the sole carbon source to enrich for organisms capable of degrading polycyclic aromatic hydrocarbons (pah) at low ph. five such bacterial strains were isolated. sequencing of the 16s rdna showed them to be members of the genera clavibacter, arthrobacter and acidocella. these organisms were all capable of growth with naphthalene as a sole carbon s ... | 2003 | 12827325 |
| genetics of metal resistance in acidophilic prokaryotes of acidic mine environments. | acidophilic bacteria inhabiting acidic mine regions cause natural leaching of sulphidic ores. they are now exploited in industrial operations for leaching of metals and beneficiation of low-grade and recalcitrant ores. recent trends emphasize application of thermoacidophiles and genetic engineering of ore-leaching bacteria for greater success in this area. this requires an in-depth understanding on the molecular genetics of these bacteria and construction of cloning vectors for them. metal resis ... | 2004 | 15274476 |
| microbial indicator groups in acidic mining lakes. | the salt composition of 14 acidic lakes was analysed, and their microbial communities were compared by their 16s rdna-based single-strand conformation polymorphism (sscp) fingerprints. the lakes were grouped into three chemically distinct types, and operational taxonomic units (otus) were identified in the 16s rdna sscp fingerprints. discriminant analysis between these groups using a multivariate approach showed that not a single organism, but rather a set of seven otus (ferromicrobium acidophil ... | 2005 | 15643943 |
| integration of metal-resistant determinants from the plasmid of an acidocella strain into the chromosome of escherichia coli dh5alpha. | acidophilic bacteria of mine origin are ideal systems for studying microbial metal resistance because of their ability to grow in the presence of high concentrations of metal salts. we have previously shown that the metal-resistant transformants obtained after transformation of escherichia coli dh5alpha with plasmid dna preparation from acidocella sp. strain gs19h did not contain any plasmid suggesting chromosomal integration of the plasmid(s) (appl environ microbiol 1997; 63: 4523-4527). the pr ... | 2005 | 15702259 |
| sulfidogenesis in low ph (3.8-4.2) media by a mixed population of acidophilic bacteria. | a defined mixed bacterial culture was established which catalyzed dissimilatory sulfate reduction, using glycerol as electron donor, at ph 3.8-4.2. the bacterial consortium comprised a endospore-forming sulfate reducing bacterium (isolate m1) that had been isolated from acidic sediment in a geothermal area of montserrat (west indies) and which had 94% sequence identity (of its 16s rrna gene) to the gram-positive neutrophile desulfosporosinus orientis, and a gram-negative (non sulfate-reducing) a ... | 2006 | 16456614 |
| acidophilic microbial communities associated with a natural, biodegraded hydrocarbon seepage. | characterization of microbial communities present in a surface petroleum seep in which hydrocarbons have been biodegraded for thousands of years in order to improve the understanding on natural petroleum biodegradation. dna was extracted from a natural, surface petroleum seep and subjected to culture independent analysis (rrna gene-based denaturing gradient gel electrophoresis and phylogenetic analysis of clone libraries). molecular analysis suggested dominance by acidophilic bacteria, especiall ... | 2006 | 16882136 |
| nucleotide sequence analysis of cryptic plasmid pam5 from acidiphilium multivorum. | plasmid pam5 of acidiphilium multivorum jcm-8867 has been completely sequenced by initial cloning of hindiii-psti fragments followed by primer walking. it has a size of 5161bp and single site for several restriction enzymes as revealed by dna sequencing. sequence analysis predicts five putative open reading frames. orf1 and orf3 show significant identity with various plasmid encoded mobilization (mob) and replication initiation (rep) proteins, respectively. the putative mob protein has several c ... | 2007 | 17363056 |
| penicillium strains as dominant degraders in soil for coffee residue, a biological waste unsuitable for fertilization. | coffee residue is an agricultural waste which inhibits the growth of several crops. therefore coffee residue-degrading microbes in soil were screened, isolated and characterized. | 2007 | 17850298 |
| "bioshrouding": a novel approach for securing reactive mineral tailings. | a novel technique ("bioshrouding") for safeguarding highly reactive sulfidic mineral tailings deposits is proposed. in this, freshly milled wastes are colonised with ferric iron-reducing heterotrophic acidophilic bacteria that form biofilms on reactive mineral surfaces, thereby preventing or minimising colonisation by iron sulfide-oxidising chemolithotrophs such as acidithiobacillus ferrooxidans and leptospirillum spp. data from initial experiments showed that dissolution of pyrite could be redu ... | 2008 | 17975731 |
| evidence that the potential for dissimilatory ferric iron reduction is widespread among acidophilic heterotrophic bacteria. | nineteen characterized strains and isolates of acidophilic heterotrophic bacteria were screened for their abilities to catalyse the reductive dissolution of the ferric iron mineral schwertmannite, under oxygen-limiting conditions. acidocella facilis, acidobacterium capsulatum, and all of the acidiphilium, acidocella and acidobacterium-like isolates that grew in liquid cultures were able to reduce iron. in contrast, neither acidisphaera rubrifaciens nor three acidisphaera-like isolates tested wer ... | 2008 | 18081844 |
| acidisoma tundrae gen. nov., sp. nov. and acidisoma sibiricum sp. nov., two acidophilic, psychrotolerant members of the alphaproteobacteria from acidic northern wetlands. | three obligately aerobic, heterotrophic bacteria, designated strains wm1t, tpb606t and tpb621, were isolated from acidic sphagnum-dominated tundra and siberian wetlands in russia. cells of these isolates were gram-negative, non-motile coccobacilli that occurred singly, in pairs or in chains, and were covered by large capsules. the novel strains were moderately acidophilic and psychrotolerant organisms capable of growth at ph 3.0-7.6 and 2-30 degrees c. cells contained numerous intracellular poly ... | 2009 | 19620354 |
| acidocella aluminiidurans sp. nov., an aluminium-tolerant bacterium isolated from panicum repens grown in a highly acidic swamp in actual acid sulfate soil area of vietnam. | an aluminium-tolerant bacterium, strain al46(t), was isolated from a waterweed, panicum repens, grown in a highly acidic swamp (ph 3) at an actual acid sulfate soil area of vietnam. cells were gram-negative, aerobic, non-spore-forming, non-motile rods (0.3 microm wide and 1.2-1.6 microm long). 16s rrna gene sequence analysis indicated that strain al46(t) belongs to the genus acidocella, class alphaproteobacteria. strain al46(t) was related most closely to the type strains of acidocella facilis a ... | 2010 | 19656936 |
| production of glycolic acid by chemolithotrophic iron- and sulfur-oxidizing bacteria and its role in delineating and sustaining acidophilic sulfide mineral-oxidizing consortia. | glycolic acid was detected as an exudate in actively growing cultures of three chemolithotrophic acidophiles that are important in biomining operations, leptospirillum ferriphilum, acidithiobacillus (at.) ferrooxidans, and at. caldus. although similar concentrations of glycolic acid were found in all cases, the concentrations corresponded to ca. 24% of the total dissolved organic carbon (doc) in cultures of l. ferriphilum but only ca. 5% of the total doc in cultures of the two acidithiobacillus ... | 2010 | 19933342 |
| ecophysiology of fe-cycling bacteria in acidic sediments. | using a combination of cultivation-dependent and -independent methods, this study aimed to elucidate the diversity of microorganisms involved in iron cycling and to resolve their in situ functional links in sediments of an acidic lignite mine lake. using six different media with ph values ranging from 2.5 to 4.3, 117 isolates were obtained that grouped into 38 different strains, including 27 putative new species with respect to the closest characterized strains. among the isolated strains, 22 st ... | 2010 | 20971876 |
| characterization of the bacterial community structure of sydney tar ponds sediment. | the sydney tar ponds is one of the largest toxic waste sites in canada. the bacterial diversity and abundance in the sydney tar ponds sediment was examined using a 16s rrna gene clone library and denaturing gradient gel electrophoresis (dgge) with four different primer sets. the clone library was grouped into 19 phylotypes that could be divided into five phyla: proteobacteria (56.9%), actinobacteria (35%), acidobacteria (4.9%), firmicutes (2.4%), and verrucomicrobia (0.8%). members of the phyla ... | 2011 | 21635217 |