| hemolymph lectin of the pearl oyster, pinctada fucata martensii: a possible non-self recognition system. | to elucidate a mutual correlation between the hemolymph lectin and hemocytes of the pearl oyster, pinctada fucata martensii, we searched for common epitopes and ligands. neither the hemocyte plasma membrane nor cytoplasm was immunoreactive to anti-hemolymph lectin antibody. the hemolymph lectin strongly bound to d-galactose and n-acetyl-d-galactosamine, and the plasma membrane of both granulocytes and agranulocytes had affinity only for d-mannose and n-acetyl-d-glucosamine-binding plant lectins. ... | 1990 | 2369965 |
| a galactose-specific lectin from the hemolymph of the pearl oyster, pinctada fucata martensii. | 1. a lectin in the serum of pinctada fucata martensii was purified by a combination of affinity chromatography on sepharose 4b coupled with bovine submaxillary gland mucine, anion exchange chromatography on mono q and gel filtration on superose 6. 2. the purified lectin was indicated to be homogeneous by polyacrylamide electrophoresis and rechromatography on mono q. 3. the purified lectin was approximately 440,000 in molecular weight and was composed of identical subunits with a molecular weight ... | 1989 | 2706936 |
| seasonal change of infective state of marine birnavirus in japanese pearl oyster pinctada fucata. | this study examines the seasonal occurrence and infective state of marine birnavirus (mabv) in cultured japanese pearl oyster (pinctada fucata). planted oysters were sampled monthly in 1997 and 1998. to detect mabv in the oysters, pcr and virus isolation were carried out. also, the indirect fluorescent antibody technique (ifat) was performed to know the organs expressing viral antigens. the detection rate of the mabv genome by pcr was low during july to october, but increased after november. thi ... | 2000 | 11087088 |
| clinical trial results on the use of a recombinant feline interferon-omega to protect japanese pearl oysters pinctada fucata martensii from akoya-virus infection. | japanese pearl oysters (akoya oyster: pinctada fucata martensii) are currently suffering mass mortalities from akoya-virus infection. in the present study, akoya oysters were injected with an anti-viral, recombinant feline interferon-omega) (rfeifn-omega) in an attempt to confer resistance to this virus. in infectivity experiments, oysters were twice injected with rfeifn-omega at 1 mega unit kg(-1) of the meat weight. they were challenged with a single inoculation of cultured akoya-virus and hel ... | 2000 | 11129377 |
| mass mortality of the japanese pearl oyster pinctada fucata martensii in relation to water temperature, chlorophyll a and phytoplankton composition. | mass mortalities of the japanese pearl oyster pinctada fucata martensii have widely occurred in western japan since 1994. the causes of these mass mortalities are at present not thoroughly understood. in this study, we investigated oyster survival in relation to some environmental factors such as water temperature, concentration of chlorophyll a and density or composition of phytoplankton. the examined mass mortality occurred from september to december 1998, and the color on the adductor muscle ... | 2001 | 11253876 |
| comparison of hemolytic activities among strains of heterocapsa circularisquama isolated in various localities in japan. | heterocapsa circularisquama (dinophyceae), a red tide dinoflagellate, is toxic to bivalve molluscs such as the pearl oyster (pinctada fucata), but no harmful effects of this alga on fish have been observed so far. we found that 7 strains of h. circularisquama showed hemolytic activities toward rabbit erythrocytes in a cell-density dependent manner, but to quite different extents. the strains which are known to be highly toxic to bivalves tend to show stronger hemolytic activities and vice versa, ... | 2002 | 11999427 |
| detection of marine birnavirus in the japanese pearl oyster pinctada fucata and seawater from different depths. | this study examines the seasonal changes of marine birnavirus (mabv) in seawater and the japanese pearl oyster pinctada fucata reared at different depths (2 and 15 m). oysters and seawater were collected in 1998, and a 2-step pcr was carried out to detect mabv. virus isolation was performed on the pcr-positive samples in the oyster. the detection rate of the mabv genome in the oyster was low during june, but increased after july at both 2 and 15 m depths. mabv was not isolated until after septem ... | 2002 | 12219977 |
| receptors for recombinant feline interferon-omega in hemocytes of the japanese pearl oyster pinctada fucata martensii. | in a previous study we determined that administration of recombinant feline interferon-omega (rfeifn-omega) could protect japanese pearl oysters pinctada fucata martensii from akoya-virus infection. our results suggested that rfeifn-omega enhanced the potential of agranulocytes to phagocytize necrotic cells and to produce collagen fibers that repair the lesions caused by the akoya-virus. in the present study, using an indirect immunofluorescence technique with an anti-rfeifn-omega rabbit serum, ... | 2002 | 12363085 |
| arsenic compounds accumulated in pearl oyster pinctada fucata. | we investigated the water-soluble arsenic compounds present in the soft tissues of both the pearl-free and the pearl-containing pearl oysters. after dividing the soft tissue into five parts, i.e., adductor muscle, foot, mantle, viscera and gill, each part was analyzed by high-performance liquid chromatography-inductively coupled plasma mass spectrometry for the arsenic compounds accumulated in it. arsenic concentration of each tissue part ranged from 22.1 to 45.7 microg g(-1) of dry tissue in th ... | 2003 | 12919784 |
| molecular cloning and expression of a pearl oyster (pinctada fucata) homologue of mammalian putative tumor suppressor qm. | the qm gene was originally identified as a putative tumor suppressor gene from a wilms' tumor cell line by subtractive hybridization assay. later studies showed that the qm protein is multifunctional, involved in cell growth and differentiation, energy metabolism, respiration, and cytoskeletal function. in this report a full-length complementary dna encoding a qm counterpart in pearl oyster (pinctada fucata) was isolated. phylogenetic analysis shows that oyster qm is more closely related to its ... | 2004 | 14508656 |
| characterization of prismalin-14, a novel matrix protein from the prismatic layer of the japanese pearl oyster (pinctada fucata). | the mollusc shell is a hard tissue consisting of calcium carbonate and organic matrices. the organic matrices are believed to play important roles in shell formation. in the present study, we extracted and purified a novel matrix protein, named prismalin-14, from the acid-insoluble fraction of the prismatic layer of the shell of the japanese pearl oyster (pinctada fucata), and determined its whole amino acid sequence by a combination of amino acid sequence analysis and ms analysis of the intact ... | 2004 | 15132736 |
| the carbonic anhydrase domain protein nacrein is expressed in the epithelial cells of the mantle and acts as a negative regulator in calcification in the mollusc pinctada fucata. | signals and organic matrix proteins secreted from the mantle are critical for the development of shells in molluscs. nacrein, which is composed of a carbonic anhydrase domain and a gly-x-asn repeat domain, is one of the organic matrix proteins that accumulates in shells. in situ hybridization revealed that nacrein was expressed in the outer epithelial cells of the mantle of the pearl oyster pinctada fucata. the recombinant nacrein protein inhibited the precipitation of calcium carbonate from a s ... | 2005 | 15795493 |
| cloning and characterization of a novel g protein beta-subunit of pearl oyster (pinctada fucata), and its interaction sites with calmodulin. | a cdna clone encoding a novel g protein beta subunit of beta1 subclass, pfgbeta1 was isolated from the pearl oyster (pinctada fucata). the deduced amino acid sequence of pfgbeta1 (341 amino acids) shares high homology to northern european squid (loligo pealei) and great pond snail (lymnaea stagnalis) pfgbeta1, while it has diverged from bovine (bos taurus) and human. the well-conserved amino acid domains in g protein beta subunit, seven wd repeats, were founded in the deduced amino acid sequence ... | 2005 | 16054410 |
| cdna cloning and characterization of a novel calmodulin-like protein from pearl oyster pinctada fucata. | calcium metabolism in oysters is a very complicated and highly controlled physiological and biochemical process. however, the regulation of calcium metabolism in oyster is poorly understood. our previous study showed that calmodulin (cam) seemed to play a regulatory role in the process of oyster calcium metabolism. in this study, a full-length cdna encoding a novel calmodulin-like protein (calp) with a long c-terminal sequence was identified from pearl oyster pinctada fucata, expressed in escher ... | 2005 | 16176264 |
| a novel carbonic anhydrase from the mantle of the pearl oyster (pinctada fucata). | a novel carbonic anhydrase (ca) has been purified from the mantle of the pearl oyster, pinctada fucata, by ammonium sulfate precipitation and affinity chromatography. its molecular mass was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page) to be approximately 38 kda. native-page shows that the novel ca can bind a fluorescent probe, 5-dimethylamino-1-naphthalenesulfonamide (dnsa), known to specifically bind carbonic anhydrase. compared to carbonic anhydrase i (cai ... | 2006 | 16406278 |
| significance of the extra c-terminal tail of calp, a novel calmodulin-like protein involved in oyster calcium metabolism. | oyster (pinctada fucata) calmodulin-like protein (calp), containing a c-terminally extra hydrophilic tail (150d-161k), is a novel protein involved in the regulation of oyster calcium metabolism. to investigate the importance of the extra fragment to the ca(2+)/mg(2+)-dependent conformational changes in the intact calp molecule and the interactions between calp and its target proteins, a truncated calp mutant (m-calp) devoid of the extended c-terminus was constructed and overexpressed in escheric ... | 2006 | 16759893 |
| does gender really matter in contaminant exposure? a case study using invertebrate models. | exposure to contaminants in the environment is indiscriminate and multiple species/populations of all sexes are potentially at risk. in this paper we examine the current information available on gender specific differences in invertebrates following exposure to environmental contaminants. because of their close association with the environment and diversity of habitats, invertebrates are uniquely at risk for adverse responses to pollutants. since 97% of all animal species are invertebrates, it w ... | 2007 | 17097631 |
| effects of cryopreservation methods on post-thaw motility of spermatozoa from the japanese pearl oyster, pinctada fucata martensii. | in order to develop cryopreservation techniques for japanese pearl oyster spermatozoa, the effects of various cryopreservation conditions on post-thaw motility were examined. spermatozoa cryopreserved with 10% methanol (met), dimethylformamide or dimethylacetamide plus 90% diluent comprising 80% seawater and 20% fetal bovine serum (fbs) showed higher percentages of post-thaw motility than those cryopreserved with 10% dimethylsulfoxide or glycerol. when spermatozoa were cryopreserved with various ... | 2007 | 17174293 |
| identification and characterization of a biomineralization related gene pfmg1 highly expressed in the mantle of pinctada fucata. | to elucidate the mechanism of nacre biomineralization, the mantle of pinctada fucata (p. fucata) from the south china sea was used. using the mantle cdna library and the ests we have cloned through suppression subtractive hybridization (ssh), ten novel genes including pfmg1 were obtained through nested pcr. bioinformative results showed that pfmg1 had a high homology (40%) with onchocerca volvulus calcium-binding protein cbp-1 and had two ef-hand calcium-binding domains from the 81st to the 93rd ... | 2007 | 17223706 |
| differential community development of fouling species on the pearl oysters pinctada fucata, pteria penguin and pteria chinensis (bivalvia, pteriidae). | a field experiment documented the development of fouling communities on two shell regions, the lip and hinge, of the pearl oyster species pinctada fucata, pteria penguin and pteria chinensis. fouling communities on the three species were not distinct throughout the experiment. however, when each species was analysed separately, fouling communities on the lip and hinge of p. penguin and p. chinensis were significantly different during the whole sampling period and after 12 weeks, respectively, wh ... | 2006 | 17290861 |
| a novel extracellular ef-hand protein involved in the shell formation of pearl oyster. | mollusk shell formation is a complicated and highly controlled calcium metabolism process. previous studies revealed that several ef-hand calcium-binding proteins actively participate in the regulation of shell mineralization. in this study, we cloned a full-length cdna encoding a novel extracellular ef-hand calcium-binding protein (named efcbp) from the pearl oyster, pinctada fucata, according to the ef-hand motifs of calmodulin. although it shares high similarity with the calmodulin family in ... | 2007 | 17451885 |
| a novel glycosylphosphatidylinositol-anchored alkaline phosphatase dwells in the hepatic duct of the pearl oyster, pinctada fucata. | alkaline phosphatases are ubiquitous enzymes involved in many important biological processes. mammalian tissue-nonspecific alkaline phosphatase (tnap) has long been thought to play an important role in bone mineralization. in this study, we identified a full-length cdna encoding a potential alkaline phosphatse from pearl oyster pinctada fucata by rt-pcr and race and designated the encoded protein as pfap. the sequence of pfap shares an overall similarity of 67% with that of human tnap. predictio ... | 2007 | 17624576 |
| characterization and in vitro mineralization function of a soluble protein complex p60 from the nacre of pinctada fucata. | a soluble protein complex p60 from the powdered nacre of pinctada fucata was extracted and partially characterized. the biological activity of the p60 on pre-osteoblast cell line mc3t3-e1 and bone marrow stroma cells (mscs) was investigated. the p60 protein from the decalcified powered nacre was solubilized with acetic acid and then purified by liquid chromatography. the p60 protein was a protein complex composed of several subunits with disulfide bridges. the known protein nacrein, and its two ... | 2007 | 17627859 |
| the structure-function relationship analysis of prismalin-14 from the prismatic layer of the japanese pearl oyster, pinctada fucata. | the mollusk shell is a hard tissue consisting of calcium carbonate and organic matrices. the organic matrices are considered to play important roles in shell formation. we have previously identified a prismatic layer-specific protein named prismalin-14, which consists of 105 amino acid residues and includes four structurally characteristic regions; a repeated sequence of pro-ile-tyr-arg, a gly/tyr-rich region and n- and c-terminal asp-rich regions. prismalin-14 showed an inhibitory activity on c ... | 2007 | 17822437 |
| gene cloning and biochemical characterization of the bmp-2 of pinctada fucata. | the bone morphogenetic proteins (bmps) constitute a subfamily of the transforming growth factor type beta (tgf-beta) supergene family. bmp-2 plays an important role not only in osteoblast differentiation but also in pattern formation during development. to determine the function of bmp-2 in pinctada fucata development and hard tissue formation, we isolated a bmp-2 genomic dna clone and the bmp-2 cdna. the deduced bmp-2 sequence consisted of 447 amino acids. the bmp-2 gene was composed of three e ... | 2008 | 18175919 |
| significance of the c-terminal globular domain and the extra tail of the calmodulin-like protein (pinctada fucata) in subcellular localization and protein-protein interaction. | calmodulin (cam) plays a very important role in many physiological processes and is highly conserved in different species. in a previous study, we successfully cloned cam and a novel calmodulin-like protein (calp) with an extra c-terminal sequence from the pearl oyster pinctada fucata and then expressed in escherichia coli. in this research, we used fluorescence confocal microscopy to analyze the protein-protein interaction between cam/calp and p21cip1, which is cloned from mammalian cells, to s ... | 2008 | 18479946 |
| molecular characterization and expression analysis of the ikappab gene from pearl oyster pinctada fucata. | inhibitor of nf-kappab (ikappab) is one important member of nf-kappab signal pathway and plays a pivotal role in regulating the innate immune response of invertebrate. herein, we described the isolation and characterization of pearl oyster pinctada fucata ikappab gene (designated as poikappab). the poikappab cdna was 1975 bp long and consisted of a 5' untranslated region (utr) of 73 bp, a 3' utr of 807 bp with three rna instability motifs (attta) and a polyadenylation signal (aataaa) at 13 nucle ... | 2009 | 19010421 |
| cloning and expression of heat shock protein 70 gene in the haemocytes of pearl oyster (pinctada fucata, gould 1850) responding to bacterial challenge. | the cdna of pearl oyster pinctada fucata hsp70 (designated pfhsp70) was cloned by est and rapid amplification of cdna ends (race) techniques. the full length of pfhsp70 cdna was 2376 bp, consisting of a 5'-terminal untranslated region (utr) of 89 bp, a 3' terminal utr of 328 bp, and an open reading frame (orf) of 1959 bp encoding a polypeptide of 652 amino acids with a theoretical molecular weight of 71.42 kda and an estimated isoelectric point of 5.18. blast analysis revealed that the pfhsp70 g ... | 2009 | 19026750 |
| molecular cloning, characterization and expression analysis of a clip-domain serine protease from pearl oyster pinctada fucata. | the clip-domain serine proteases (sps) are the essential components of extracellular signaling cascade in various biological processes, especially in embryonic development and the innate immune responses of invertebrate. herein, we described the isolation and characterization of pearl oyster pinctada fucata clip-domain sp gene (designated as posp). the posp cdna was 1080 bp long and consisted of a 5'-untranslated region (utr) of 13 bp, a 3'-utr of 68 bp with a polyadenylation signal (aataaa) at ... | 2009 | 19254766 |
| the structure-function relationship of msi7, a matrix protein from pearl oyster pinctada fucata. | we previously identified a matrix protein, msi7, from pearl oyster pinctada fucata. according to the structural analysis, the dgd site in the n-terminal of msi7 is crucial for its role in the shell formation. in this study, we expressed a series of recombinant msi7 proteins, including the wild-type and several mutants directed at the dgd site, using an escherichia coli expression system to reveal the structure-function relationship of msi7. furthermore, in vitro crystallization, crystallization ... | 2009 | 19902130 |
| cloning and characterization of the activin like receptor 1 homolog (pf-alr1) in the pearl oyster, pinctada fucata. | the signal transduction mechanisms in mollusks are still elusive since the genome information is incomplete and cell lines are not available. in previous study, we cloned a highly conserved smad3 homolog (designated as pf-smad3) from the pearl oyster, pinctada fucata. it seems that transforming growth factor beta (tgfbeta) signaling may play similar roles in the oyster as in vertebrate. here we report a cdna encoding an activin like receptor 1 homolog (designated as pf-alr1) of the oyster, anoth ... | 2010 | 20226263 |
| molecular characterization and expression analysis of interferon-gamma-inducible lysosomal thiol reductase (gilt) gene from pearl oyster pinctada fucata. | interferon-gamma-inducible lysosomal thiol reductase (gilt) is an important thiol reductase, involved in class, mhc-restricted antigen processing by catalyzing disulfide bond reduction in mammals. herein, we describe the identification and characterization of pearl oyster pinctada fucata gilt (designated as pogilt). the pogilt cdna was 1273bp long and consisted of a 5'-untranslated region (utr) of 24bp, a 3'-utr of 484bp with two cytokine rna instability motifs (attta), and an open reading frame ... | 2010 | 20444427 |
| molecular characterization and expression analysis of cathepsin l1 cysteine protease from pearl oyster pinctada fucata. | cathepsin l is one of the crucial enzyme superfamilies and involved in the immune responses. in this study, a cdna encoding cathepsin l cysteine protease was identified and characterized from pearl oyster pinctada fucata (designated as pocl1). the pocl1 cdna was 1160 bp long and consisted of a 5'-untranslated region (utr) of 15 bp, a 3'-utr of 149 bp with a polyadenylation signal (aataaa) at 11 nucleotides upstream of the poly(a) tail, and an open reading frame (orf) of 996 bp encoding a polypep ... | 2010 | 20573562 |
| a multidomain galectin involved in innate immune response of pearl oyster pinctada fucata. | galectins could specifically bind to β-galactoside residues and play crucial roles in innate immune responses of vertebrates and invertebrates. in this study, the cdna of a galectin with multiple carbohydrate-recognition domains (crds) was cloned from pearl oyster pinctada fucata (designated as pogal). pogal cdna was 2138bp long and consisted of a 5'-untranslated region (utr) of 120bp, a 3'-utr of 350bp with two cytokine rna instability motifs (attta), and an open reading frame (orf) of 1668bp e ... | 2011 | 20813129 |
| pinctada fucata mantle gene 3 (pfmg3) promotes differentiation in mouse osteoblasts (mc3t3-e1). | nacre is secreted from the mantle of pearl oysters. in vivo and in vitro experiments have demonstrated that water-soluble extracts of nacre stimulate osteoblast differentiation and matrix mineralization, but the component responsible for this activity is unclear. it was reported that pinctada fucata mantle gene 3 (pfmg3) with an n-terminal signal peptide could be secreted into the nacre of p. fucata. here we report that pfmg3 is specifically expressed at the outer fold of the mantle and could pr ... | 2010 | 21109014 |
| a macrophage migration inhibitory factor like oxidoreductase from pearl oyster pinctada fucata involved in innate immune responses. | macrophage migration inhibitory factor (mif) is an important cytokine and plays a crucial role as a pivotal regulator of innate immunity. in this study, a mif cdna was identified and characterized from the pearl oyster pinctada fucata (designated as pomif). the full-length of pomif was 1544 bp and consisted of a 5'-untranslated region (utr) of 45 bp, a 3'-utr of 1139 bp with a polyadenylation signal (aataaa) at 12 nucleotides upstream of the poly (a) tail. the open reading frame (orf) of pomif w ... | 2011 | 21496487 |
| cloning and expression of an actin gene in the haemocytes of pearl oyster (pinctada fucata, gould 1850). | an actin gene (designated pfact1) of pearl oyster, pinctada fucata, was cloned from haemocytes by the techniques of homological cloning and rapid amplification of cdna ends (race). the full length of pfact1 cdna was 1608-ábp in length, having a 5' untranslated region (utr) of 82-ábp, a 3' utr of 395-ábp, and an open reading frame (orf) of 1131-ábp encoding a polypeptide of 376 amino acids with a predicted molecular weight of 41.76-ákda and an estimated isoelectric point of 5.29. sequence analysi ... | 2008 | 21798155 |
| MIF from mussel: Coding sequence, phylogeny, polymorphism, 3D model and regulation of expression. | Three macrophage migration inhibitory factor (MIF)-related sequences were identified from a Mytilus galloprovincialis EST library. The consensus sequence included a 5'-UTR of 32 nucleotides, the complete ORF of 345 nucleotides, and a 3'-UTR of 349 nucleotides. As for other MIFs, M. galloprovincialis ORF does not include any signal or C-terminus extensions. The translated sequence of 115 amino acids possesses a molecular mass of 12,681.4, a pI of 6.27 and a stability index of 21.48. Its 3D struct ... | 2011 | 22085783 |
| molecular characterization and mrna expression of catalase from pearl oyster pinctada fucata. | catalase (ec 1.11.1.6) is an important antioxidant enzyme that protects aerobic organisms against oxidative damage by degrading hydrogen peroxide to water and oxygen. in the present study, a catalase cdna of peal oyster pincatada fucata (designated as pocat) is cloned and characterized by expressed sequence tag (est) and rapid amplification of cdna ends (race) methods. pocat is 2428 bp long and consists of a 5'-utr of 140 bp, an unusually long 3'-utr of 749 bp, and an open reading frame (orf) of ... | 2011 | 22118636 |
| a tandem-repeat galectin involved in innate immune response of the pearl oyster pinctada fucata. | the cdna of a tandem-repeat galectin from the pearl oyster pinctada fucata (designated pfgal) was cloned by expressed sequence tag (est) and rapid amplification of cdna ends (race) techniques. the full-length cdna of pfgal was 1386bp, consisting of a 5' untranslated region (utr) of 26bp, a 3' utr of 313bp, and an open reading frame (orf) of 1047bp encoding a polypeptide of 348 amino acids with a predicted molecular weight of 38.09kda and theoretical isoelectric point of 8.49. similar to other ta ... | 2011 | 21867976 |
| cdna cloning and mrna expression of a tandem-repeat galectin (pogal2) from the pearl oyster, pinctada fucata. | galectins can recognize and specifically bind to β-galactoside residues, playing crucial roles in innate immune responses of vertebrates and invertebrates. we cloned the cdna of a tandem-repeat galectin from the pearl oyster pinctada fucata (designated as pogal2). pogal2 cdna is 1347 bp long and consists of a 5'-untranslated region (utr) of 3 bp, a 3'-utr of 297 bp with one cytokine rna instability motif (attta), and an open reading frame of 1047 bp, encoding a polypeptide of 349 amino acids, wi ... | 2011 | 21948759 |
| water soluble bioactives of nacre mediate antioxidant activity and osteoblast differentiation. | the water soluble matrix of nacre is a proven osteoinductive material. in spite of the differences in the biomolecular compositions of nacre obtained from multiple species of oysters, the common biochemical properties of those principles substantiate their biological activity. however, the mechanism by which nacre stimulates bone differentiation remains largely unknown. since the positive impact of antioxidants on bone metabolism is well acknowledged, in this study we investigated the antioxidan ... | 2013 | 24367677 |
| pfirr interacts with hrigf-i and activates the map-kinase and pi3-kinase signaling pathways to regulate glycogen metabolism in pinctada fucata. | the insulin-induced mitogen-activated protein kinase (mapk) and phosphatidylinositol 3-kinase (pi3k) pathways are major intracellular signaling modules and conserved among eukaryotes that are known to regulate diverse cellular processes. however, they have not been investigated in the mollusk species pinctada fucata. here, we demonstrate that insulin-related peptide receptor of p. fucata (pfirr) interacts with human recombinant insulin-like growth factor i (hrigf-i), and stimulates the mapk and ... | 2016 | 26911653 |
| pfsmad4 plays a role in biomineralization and can transduce bone morphogenetic protein-2 signals in the pearl oyster pinctada fucata. | mollusca is the second largest phylum in nature. the shell of molluscs is a remarkable example of a natural composite biomaterial. biomineralization and how it affects mollusks is a popular research topic. the bmp-2 signaling pathway plays a canonical role in biomineralization. smad4 is an intracellular transmitter in the bmp signaling pathway in mammals, and some genomic data show smad4's involvement in bmp signaling in invertebrates, but whether smad4 plays a conservative role in pearl oyster, ... | 2016 | 27113217 |
| microarray: a global analysis of biomineralization-related gene expression profiles during larval development in the pearl oyster, pinctada fucata. | the molluscan pinctada fucata is an important pearl-culturing organism to study biomineralization mechanisms. several biomineralization-related genes play important roles regulating shell formation, but most previous work has focused only on their functions in adult oysters. few studies have investigated biomineralization during larval development, when the shell is initially constructed and formed until the juvenile stage in dissoconch shells. here, we report, for the first time, a global gene ... | 2015 | 25927556 |
| draft genome of the pearl oyster pinctada fucata: a platform for understanding bivalve biology. | the study of the pearl oyster pinctada fucata is key to increasing our understanding of the molecular mechanisms involved in pearl biosynthesis and biology of bivalve molluscs. we sequenced ~1150-mb genome at ~40-fold coverage using the roche 454 gs-flx and illumina gaiix sequencers. the sequences were assembled into contigs with n50 = 1.6 kb (total contig assembly reached to 1024 mb) and scaffolds with n50 = 14.5 kb. the pearl oyster genome is at-rich, with a gc content of 34%. dna transposons, ... | 2012 | 22315334 |
| a cdna cloning of a novel alpha-class tyrosinase of pinctada fucata: its expression analysis and characterization of the expressed protein. | tyrosinase plays an important role in the formation of the shell matrix and melanin synthesis in mollusks shells. a cdna clone encoding a 47 kda protein was isolated from the pearl oyster pinctada fucata. the cdna was 1,957 base pairs long and encodes a 417 residue protein that has extensive sequence identity with tyrosinase (polyphenol oxidase: ec 1.14.18.1). this tyrosinase-like protein, termed pfty, contains an n-terminal signal sequence and the two copper-binding domain signatures (cua and c ... | 2014 | 24818013 |
| molecular cloning and characterization of full-length cdna of calmodulin gene from pacific oyster crassostrea gigas. | the shell of the pearl oyster (pinctada fucata) mainly comprises aragonite whereas that of the pacific oyster (crassostrea gigas) is mainly calcite, thereby suggesting the different mechanisms of shell formation between above two mollusks. calmodulin (cam) is an important gene for regulating the uptake, transport, and secretion of calcium during the process of shell formation in pearl oyster. it is interesting to characterize the cam in oysters, which could facilitate the understanding of the di ... | 2016 | 27703977 |
| deep sequencing of ests from nacreous and prismatic layer producing tissues and a screen for novel shell formation-related genes in the pearl oyster. | despite its economic importance, we have a limited understanding of the molecular mechanisms underlying shell formation in pearl oysters, wherein the calcium carbonate crystals, nacre and prism, are formed in a highly controlled manner. we constructed comprehensive expressed gene profiles in the shell-forming tissues of the pearl oyster pinctada fucata and identified novel shell formation-related genes candidates. | 2011 | 21731681 |
| differential gene expression during larval metamorphic development in the pearl oyster, pinctada fucata, based on transcriptome analysis. | p. fucata experiences a series of transformations in appearance, from swimming larvae to sessile juveniles, during which significant changes in gene expression likely occur. thus, p. fucata could be an ideal model in which to study the molecular mechanisms of larval metamorphosis during development in invertebrates. to study the molecular driving force behind metamorphic development in larvae of p. fucata, transcriptomes of five larval stages (trochophore, d-shape, umbonal, eyespots, and spats) ... | 2016 | 27843935 |
| recombinant production of a shell matrix protein in escherichia coli and its application to the biomimetic synthesis of spherulitic calcite crystals. | to overcome the limited production capability of shell matrix proteins and efficiently conduct in vitro caco3 biomineralization studies, a putative recombinant shell matrix protein was prepared and characterized. | 2016 | 26758877 |
| n16, a nacreous protein, inhibits osteoclast differentiation and enhances osteogenesis. | n16 is a protein from the nacreous layer of pinctada fucata, a pearl oyster. it has been found to promote biomineralization, and we hypothesized that it also plays a role in bone metabolism. the cdna of n16 was cloned and expressed in escherichia coli to produce n16 protein, which was purified to high homogeneity by ion-exchange and gel filtration columns. the effects of n16 on osteoclast differentiation and osteogenesis were clarified using the murine preosteoclast cell line raw 264.7 and the p ... | 2016 | 26741297 |
| identification and expression characterization of three wnt signaling genes in pearl oyster (pinctada fucata). | the wnt signaling pathway plays an important role in animal development and in the biomineralization process. at present, although the biomineralization mechanism in pearl oyster (pinctada fucata) has been extensively studied, there is little research on the wnt signaling pathway in pearl oyster. to understand the potential role of the wnt signaling pathway in pearl oyster, we cloned and sequenced three genes from the wnt signaling pathway in pearl oyster that encode the following proteins: β-ca ... | 2017 | 26969109 |
| three stats are involved in the regulation of the expression of antimicrobial peptides in the triangle sail mussel, hyriopsis cumingii. | janus kinase (jak) and signal transducers and activators of transcription (stat) signaling pathway is associated in antiviral and antibacterial immune response. previous studies primarily investigated the function of stats in mammals. for most invertebrates, only one stat was found in each species, such as stat92e was found in drosophila melanogaster. the studies, which focus on the functional difference between various stats in the same species of invertebrate, are limited. in the present study ... | 2017 | 28214598 |
| an alpha-2 macroglobulin in the pearl oyster pinctada fucata: characterization and function in hemocyte phagocytosis of vibrio alginolyticus. | alpha-2 macroglobulin (α2m) is a ubiquitous protease inhibitor and considered to be an evolutionarily conserved constituent of innate host defence system. here, an α2m gene (designated as pfα2m) was obtained from the pearl oyster pinctada fucata by rt-pcr, pcr walking and rapid amplification of cdna ends (race). the pfα2m cdna consists of 6394 bp with an open reading frame (orf) of 5745 bp encoding a protein of 1914 amino acids with a 19 residues signal peptide. pfα2m sequence contains three put ... | 2016 | 27346151 |
| transcriptome analysis of the pearl oyster (pinctada fucata) hemocytes in response to vibrio alginolyticus infection. | the pearl oyster pinctada fucata is cultured widely for production of marine pearls in china, while mass mortalities, likely related to pathogenic infections, have occurred frequently in juvenile, mother and operated oysters. to address this issue, understanding host defense mechanisms of p. fucata against pathogenic challenge is extremely important. in the present study, a comparative analysis of hemocyte transcriptomes of p. fucata before and after vibrio alginolyticus infection was conducted ... | 2016 | 26363408 |
| molecular characterization, expression and function analysis of a five-domain kazal-type serine proteinase inhibitor from pearl oyster pinctada fucata. | serine proteinase inhibitors represent an expanding superfamily of endogenous inhibitors that are regulate proteolytic events and involved in a variety of physiological and immunological processes. a five-domain kazal-type serine proteinase inhibitor (pokspi) was identified and characterized from pearl oyster pinctada fucata based on expressed sequence tag (est) analysis. the full-length cdna was 737 bp with an open reading frame (orf) 660 bp encoding a 219 amino acid protein a theoretical molec ... | 2014 | 24378679 |
| genomic structure, characterization and expression analysis of a manganese superoxide dismutase from pearl oyster pinctada fucata. | manganese superoxide dismutase (mnsod) is a major component of the cellular defense mechanisms against oxidative damage. we cloned and analyzed the expression pattern and genomic structure of the mnsod gene of pearl oyster pinctada fucata, hereafter designated as pomnsod. the full-length pomnsod cdna was 1080 bp in length and consisted of a 5'-untranslated region (utr) of 222 bp, a 3'-utr of 318 bp with a polyadenylation signal (aataaa) at 15 nucleotides upstream of the poly (a) tail, and an ope ... | 2013 | 23880235 |
| molecular cloning and characterization of class i nf-κb transcription factor from pearl oyster (pinctada fucata). | nf-κb transcription factors play central roles in many important physiological and pathological processes including innate immune responses. here we report the cloning of an nf-κb transcription factor, pfrelish from pearl oyster pinctada fucata, one of the most important bivalve mollusks for seawater pearl production. pfrelish full-length cdna is 3916 bp with an open reading frame of 3558 bp encoding a putative protein of 1186 amino acids. the deduced pfrelish contains a n-terminal rhd, a nucleu ... | 2012 | 22796487 |
| molecular cloning, characterization and expression analysis of tumor necrosis factor receptor-associated factor 3 (traf3) from pearl oyster pinctada fucata. | traf3 is a highly versatile regulator that negatively regulates jnk and alternative nuclear factor-κb signalling, but positively controls type i interferon production. to investigate traf3 function in innate immune responses among invertebrate especially mollusk, we characterized traf3 (pftraf3) from pearl oyster pinctada fucata, one of the most important bivalve mollusks for seawater pearl production. pftraf3 cdna is 2261 bp with an open reading frame of 1623 bp encoding a putative protein of 5 ... | 2012 | 22796485 |
| cathepsin l is an immune-related protein in pacific abalone (haliotis discus hannai)--purification and characterization. | cathepsin l, an immune-related protein, was purified from the hepatopancreas of pacific abalone (haliotis discus hannai) by ammonium sulfate precipitation and column chromatographies of sp-sepharose and sephacryl s-200 hr. purified cathepsin l appeared as two bands with molecular masses of 28.0 and 28.5 kda (namely cathepsin la and lb) on sds-page under reducing conditions, suggesting that it is a glycoprotein. peptide mass fingerprinting (pmf) analysis revealed that peptide fragments of 95 amin ... | 2015 | 26549175 |
| marinegenomicsdb: an integrated genome viewer for community-based annotation of genomes. | we constructed a web-based genome annotation platform, marinegenomicsdb, to integrate genome data from various marine organisms including the pearl oyster pinctada fucata and the coral acropora digitifera. this newly developed viewer application provides open access to published data and a user-friendly environment for community-based manual gene annotation. development on a flexible framework enables easy expansion of the website on demand. to date, more than 2000 genes have been annotated usin ... | 2013 | 24125644 |
| cloning and characterization of the shell matrix protein shematrin in scallop chlamys farreri. | the shematrin family is unique to the organic matrices of pearl oyster shells, containing repetitive, low-complexity domains designated as xgnx (where x is a hydrophobic amino acid). current studies suggested that shematrins are framework proteins in the prismatic layer of pinctada fucata; however, the exact function of shematrin during shell formation is unclear. in this study, we cloned and characterized shematrin, a protein highly homologous to shematrin-2, from the mantle tissue of scallop ( ... | 2014 | 25001481 |
| transcriptome analysis of the freshwater pearl mussel (cristaria plicata) mantle unravels genes involved in the formation of shell and pearl. | cristaria plicata, a bivalve widespread in eastern asia fresh water, is utilized as the freshwater pearl mussel in china. with a high economic value in pearl production, it is also an ideal object used for the studies on biomineralization in freshwater. in the research, we performed a large-scale sequencing of cristaria plicata mantle transcriptome using illumina hiseq™ 2500, obtaining 98,501 unigenes with 67,817,724 bases. 22.28 and 16.64% of the unigenes were annotated in the nr and swiss-prot ... | 2017 | 27987057 |
| bivalve-specific gene expansion in the pearl oyster genome: implications of adaptation to a sessile lifestyle. | bivalve molluscs have flourished in marine environments, and many species constitute important aquatic resources. recently, whole genome sequences from two bivalves, the pearl oyster, pinctada fucata, and the pacific oyster, crassostrea gigas, have been decoded, making it possible to compare genomic sequences among molluscs, and to explore general and lineage-specific genetic features and trends in bivalves. in order to improve the quality of sequence data for these purposes, we have updated the ... | 2016 | 26900483 |
| repertoire and evolution of tnf superfamily in crassostrea gigas: implications for expansion and diversification of this superfamily in mollusca. | tumor necrosis factor superfamily (tnfsf) members represent a group of cytokines participating in diverse immunological, pathological and developmental pathways. however, compared with deuterostomia and cnidaia, the composition and evolution of tnf homologous in protostomia are still not well understood. in the present study, a total of 81 tnf superfamily (tnfsf) genes from 15 mollusk species, including 23 tnfsf genes from crassostrea gigas, were surveyed by genome-wide bioinformatics analysis. ... | 2015 | 25910814 |
| molecular characterization and analysis of a putative 5-ht receptor involved in reproduction process of the pearl oyster pinctada fucata. | 5-ht (5-hydroxytryptamine; serotonin) has been linked to a variety of biological roles including gonad maturation and sequential spawning in bivalve molluscs. to gain a better understanding of the effects of 5-ht on developmental regulation in the pearl oyster pinctada fucata, the isolation, cloning, and expression of the 5-ht receptor was investigated in this study. a full-length cdna (2541 bp) encoding a putative 5-ht receptor (5-htpf) of 471 amino acids was isolated from the ovary of the pear ... | 2014 | 24852353 |
| crassostrea gigas ferritin: cdna sequence analysis for two heavy chain type subunits and protein purification. | ferritin has been shown as being the principal iron storage in the majority of living organisms. in marine species, ferritin is also involved in high-level accumulation of (210)po. as part of our work on the investigation of these radionuclides' concentration in natural environment, ferritin was searched at the gene and protein level. ferritin was purified from the visceral mass of the oyster crassostrea gigas by ion-exchange chromatography and hplc. sds-page revealed one band of 20 kda. an expr ... | 2004 | 15315822 |
| sequence of the pearl oyster carbonic anhydrase-related protein and its evolutionary implications. | carbonic anhydrases are conserved in vertebrates and invertebrates, and a noncatalytic carbonic anhydrase-related protein viii (carp viii) has been found in deuterostomes and the phylum placozoa. i isolated a cdna encoding a noncatalytic carp from the mantle of the pearl oyster pinctada fucata. the polypeptide (carp-1) predicted from the nucleotide sequence shares 44-60% identity with known carp viii sequences, and its phylogenetic analysis showed that p. fucata formed a single group with deuter ... | 2012 | 21964518 |
| evolutionary aspects of variability in bhlh orthologous families: insights from the pearl oyster, pinctada fucata. | basic helix-loop-helix (bhlh) transcription factors play significant roles in multiple biological processes in metazoan cells. in recent work, we showed that three orthologous hlh families, pearl, amber, and peridot, have apparently been lost in the drosophila melanogaster, caenorhabditis elegans, and homo sapiens lineages. to further address the gain and loss of bhlh proteins during bilaterian evolution, we examined the genome of the pearl oyster, pinctada fucata, which has recently been sequen ... | 2013 | 24125650 |
| pf-sp8/9, a novel member of the specificity protein family in pinctada fucata, potentially participates in biomineralization. | specificity protein (sp) belong to a transcription factor family that contains nine subgroups with essential functions in development, including skeletogenesis, tooth development, neural tube closure, and limb formation. in molluscs, functions of the sp protein family members have not been reported in detail. in this study, we report the first sp protein-encoding gene in pinctada fucata. we named the translated protein pf-sp8/9, based on the phylogenetic development tree constructed using sp pro ... | 2016 | 27178782 |
| extensible byssus of pinctada fucata: ca(2+)-stabilized nanocavities and a thrombospondin-1 protein. | the extensible byssus is produced by the foot of bivalve animals, including the pearl oyster pinctada fucata, and enables them to attach to hard underwater surfaces. however, the mechanism of their extensibility is not well understood. to understand this mechanism, we analyzed the ultrastructure, composition and mechanical properties of the p. fucata byssus using electron microscopy, elemental analysis, proteomics and mechanical testing. in contrast to the microstructures of mytilus sp. byssus, ... | 2015 | 26446436 |