experimental pestivirus infections in pregnant goats.fifty pregnant goats, inoculated intramuscularly at different gestational stages with a non-cytopathic ovine pestivirus or a cytopathic bovine pestivirus, all developed pestivirus-neutralizing antibodies within 5 weeks of inoculation. the incidence of reproductive failure was similar for the two agents. parturition at term with only healthy kids occurred in 13 (26 per cent) of the goats. viable kids were not born to any of the 17 goats inoculated at about day 40 of gestation. three of the 17 del ...19911663955
contamination of a live virus vaccine against pseudorabies (aujeszky's disease) by an ovine pestivirus pathogen for the pig. 19882852911
pathology in the ovine foetus caused by an ovine pestivirus.homogenised tissues or tissue culture supernatant fluid containing a noncytopathic pestivirus obtained from a lamb with a neurologic form of border disease, were inoculated into ewes at different stages of pregnancy. foetal death occurred in 9 ewes of those inoculated between 19 and 47 days of pregnancy while 3 ewes did not lamb. eight of the foetuses were aborted between 77 and 132 days of pregnancy; of these 6 were autolysed or mummified and one had arthrogryposis. the one full-term dead lamb ...19836311154
further characterization of border disease virus isolates: evidence for the presence of more than three species within the genus pestivirus.the molecular analysis of three ovine pestivirus strains revealed the existence of two distinct groups of sheep-derived pestiviruses, namely "true" border disease virus strains (bdv) and bovine viral diarrhea virus (bvdv)-like strains. as an extension of these studies rt-pcr and nucleotide sequencing of the autoprotease (npro) and nucleocapsid protein (c) encoding regions of additional serologically defined ovine pestivirus strains were performed. a comparison of npro and c revealed that three o ...19957747470
molecular characterization of border disease virus, a pestivirus from sheep.three serologically different pestivirus strains isolated from sheep were selected for molecular analysis. cdna and deduced amino acid sequences of the genomic regions encoding glycoproteins e1 and e2 were obtained from the three strains. a comparison with amino acid sequences of bovine viral diarrhea virus (bvdv) and classical swine fever virus (csfv) revealed that one of the three ovine pestivirus strains can be grouped together with bvdv. the other two strains, however, were clearly different ...19948291236
genetic characterization of ruminant pestiviruses: sequence analysis of viral genotypes isolated from sheep.historically, the genus pestivirus was believed to contain three species of viruses; bovine viral diarrhea virus (bvdv), border disease virus (bdv) and classical swine fever virus (csfv). however, based on limited sequence analysis of a small number of pestiviral isolates from domestic livestock, evidence has recently emerged indicating that at least four distinct genotypes exist. in an attempt to gain a better understanding of the degree of viral variation among ruminant pestiviruses, the entir ...19979037733
molecular characterization of ovine pestiviruses.forty-two ovine pestivirus isolates, collected over a period of 18 years, were compared by phylogenetic analysis. the viruses were mostly field isolates from britain; two others originated from sweden and two from new zealand. rt-pcr products were obtained from two genomic regions, one within the 5'-noncoding (5'-nc) region, and the other encompassing parts of the p20 (npro) and c coding regions. direct sequencing of the 5'-nc pcr products, followed by computer-assisted phylogenetic analysis, di ...19979129644
ovine pestiviruses: their taxonomic status revealed by palindromic nucleotide substitutions.we examined previously identified border disease virus (bdv) strains by using a newly proposed genotyping procedure based on palindromic nucleotide substitutions (pns) in the 5'-untranslated region (utr), and found 22 (41.5%) out of 53 strains of bdv in the nucleotide sequence databases are not of bdv. all the 22 ovine pestivirus strains were allocated to the bvdv species according to the pns, and were compared with reference strains of pestivirus 1 (bvdv-ia,-ib, and-ic genovars), pestivirus 2 ( ...199910591495
a rt-pcr assay for the rapid recognition of border disease virus.a reverse transcription--polymerase chain reaction (rt-pcr) method was developed for the specific detection of border disease virus (bdv), using the primers pbd1 and pbd2 flanking a 225 bp dna fragment, selected from the 5'noncoding region of the pestivirus genome. in tests on 70 pestiviruses it was shown to be bdv-specific. a closed, one-tube nested rt-pcr method employing general pestivirus outer primers (324 and 326), and the same bdv-specific inner primers (pbd1 and pbd2) in conjunction with ...200010958243
genetic and antigenic characterization of novel pestivirus genotypes: implications for classification.currently, the genus pestivirus comprises the four approved species bovine viral diarrhea virus 1 (bvdv-1), bvdv-2, classical swine fever virus (csfv), and border disease virus (bdv) and one tentative fifth species represented by a single strain (h138) isolated from a giraffe in kenya more than 30 years ago. to further address the issue of heterogeneity of pestiviruses we have determined the entire n(pro) and e2 coding sequences for several new pestivirus isolates. interestingly, phylogenetic an ...200312832207
genetic characterization of ovine pestiviruses isolated in france, between 1985 and order to investigate the genetic heterogeneity of ovine pestivirus isolates in france, 32-clinical samples collected between 1985 and 2006, previously detected positive by indirect immuno-fluorescence for the presence of border disease virus (bdv), were tested by reverse transcription-polymerase chain reaction (rt-pcr). parts of the 5'-non-coding region (5'-ncr) and the npro-coding region were successfully amplified from 23 samples. an internal control was designed and used to assess the vali ...200818299176
clinical and laboratorial findings in pregnant ewes and their progeny infected with border disease virus (bdv-4 genotype).to evaluate the pathogenicity of local isolates of ovine pestiviruses (bdv-4 genotype), 13 virus- and antibody-negative, artificially inseminated pregnant ewes were challenged on days 108 (5 ewes), 76 (5 ewes) and 55 of pregnancy (3 ewes) with 2 ml of ovine pestivirus containing 10(6) tcid(50). viraemia was detected by rt-pcr from 2 to 15 days pi in most ewes. no abortion due to the infection was observed but the number of stillbirths was high (32%), and bodyweight at lambing was significantly r ...200918755485
genetic and antigenic typing of border disease virus (bdv) isolates from italy reveals the existence of a novel bdv group.border disease virus belongs to the pestivirus genus, within the family flaviviridae. genetic analysis of pestiviruses isolated from sheep in continental europe have led to the proposal that bdv isolates are segregated into at least seven clusters. in 2005 the molecular analysis of an italian caprine bdv strain provided evidence for the presence of an atypical pestivirus, which may represent the first member of a putative novel pestivirus sub-group. to further build on this study, ovine pestivir ...201120656426
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