| structure of fuscopeptins, phytotoxic metabolites of pseudomonas fuscovaginae. | the structure of the fuscopeptins, bioactive lipodepsipeptides produced in culture by the gramineae pathogen pseudomonas fuscovaginae, has been determined. the combined use of fab mass spectroscopy nmr spectroscopy and chemical and enzymatic procedures allowed one to define a peptide moiety corresponding to z-dhb-d-pro-l-leu-d-ala-d-ala-d-ala-d-ala-d-val-gly-d-ala-d-val-d-ala-d- val-z-dhb-da-thr-l-ala-l-dab-d-dab-l-phe with the terminal carboxyl group closing a macrocyclic ring on the hydroxyl g ... | 1996 | 8601458 |
| modulation of plant plasma membrane h+-atpase by phytotoxic lipodepsipeptides produced by the plant pathogen pseudomonas fuscovaginae. | pseudomonas fuscovaginae produces the lipodepsipeptides syringotoxin, fuscopeptin a and fuscopeptin b concurrently. these phytotoxins inhibit acidification of the external medium by fusicoccin-treated rice leaf sheath discs. when tested in vitro on h+-atpase of rice shoot plasma membranes, syringotoxin and its structural analogue syringomycin, produced by p. syringae pv. syringae, displayed a double effect. at low concentrations they stimulated the atpase activity of native right-side-out membra ... | 1998 | 9675287 |
| biological and molecular detection of toxic lipodepsipeptide-producing pseudomonas syringae strains and pcr identification in plants | toxin-based identification procedures are useful for differentiating pseudomonas syringae pathovars. a biological test on peptone-glucose-nacl agar in which the yeast rhodotorula pilimanae was used proved to be more reliable for detecting lipodepsipeptide-producing strains of p. syringae than the more usual test on potato dextrose agar in which geotrichum candidum is used. a pcr test performed with primers designed to amplify a 1, 040-bp fragment in the coding sequence of the syrd gene, which wa ... | 1999 | 10223977 |
| conformations in solution of the fuscopeptins. phytotoxic metabolites of pseudomonas fuscovaginae. | fuscopeptins are phytotoxic amphiphilic lipodepsipeptides containing 19 amino acid residues. they are produced by the plant pathogenic bacterium pseudomonas fuscovaginae in two forms, a and b, which differ only in the number of methylene groups in the fatty acid chain. their covalent structure and biological properties have been reported previously. cd and nmr spectroscopy investigations in solution revealed the absence of identifiable elements of secondary and tertiary structure for these molec ... | 1999 | 10561589 |
| siderophore typing, a powerful tool for the identification of fluorescent and nonfluorescent pseudomonads. | a total of 301 strains of fluorescent pseudomonads previously characterized by conventional phenotypic and/or genomic taxonomic methods were analyzed through siderotyping, i.e., by the isoelectrophoretic characterization of their main siderophores and pyoverdines and determination of the pyoverdine-mediated iron uptake specificity of the strains. as a general rule, strains within a well-circumscribed taxonomic group, namely the species pseudomonas brassicacearum, pseudomonas fuscovaginae, pseudo ... | 2002 | 12039729 |
| pseudomonas salomonii sp. nov., pathogenic on garlic, and pseudomonas palleroniana sp. nov., isolated from rice. | a total of 26 strains, including 15 strains isolated from garlic plants with the typical symptoms of 'café au lait' disease and 11 strains isolated from diseased or healthy rice seeds and sheaths infested by pseudomonas fuscovaginae, were compared with 70 type or reference strains of oxidase-positive pathogenic or non-pathogenic fluorescent pseudomonads. the strains were characterized by using a polyphasic taxonomic approach. numerical taxonomy of phenotypic characteristics showed that the garli ... | 2002 | 12508870 |
| high-performance liquid chromatography analyses of pyoverdin siderophores differentiate among phytopathogenic fluorescent pseudomonas species. | the relationship of pyoverdins produced by 41 pathovars of pseudomonas syringae and by phytopathogenic pseudomonas species was investigated. a high-performance liquid chromatography method for analyzing the culture medium proved to be superior to isoelectric focusing for detecting pyoverdin production, for differentiating slightly different pyoverdins, and for differentiating atypical from typical fe(iii)-chelated pyoverdins. nonfluorescent strains were found in pseudomonas amygdali, pseudomonas ... | 2003 | 12571041 |
| pseudomonas moraviensis sp. nov. and pseudomonas vranovensis sp. nov., soil bacteria isolated on nitroaromatic compounds, and emended description of pseudomonas asplenii. | two strains of gram-negative bacteria isolated from soil by selective enrichment with nitroaromatics were subjected to a polyphasic taxonomic study. on the basis of 16s rrna gene sequence analysis, the two strains were found to belong to the genus pseudomonas, within the gammaproteobacteria. strain 1b4t shared the highest sequence similarity with pseudomonas koreensis dsm 16610t (99.5%) and pseudomonas jessenii ccm 4840t (99.3%), and strain 2b2t with pseudomonas asplenii dsm 17133t (98.9%), pseu ... | 2006 | 17082407 |
| fuscopeptins, antimicrobial lipodepsipeptides from pseudomonas fuscovaginae, are channel forming peptides active on biological and model membranes. | fp-a and fp-b are ldps produced by the plant pathogen pseudomonas fuscovaginae. as expected from their primary structure, they shared a similar mechanism of action with the better characterized sps, synthesized by strains of pseudomonas syringae pv. syringae. indeed, they displayed hemolytic activity on human erythrocytes and were able to induce calcein release from luvs: the effect was dependent on the concentration of the fps and the lipid composition of the liposome and, in particular, it inc ... | 2008 | 18085513 |
| the plant pathogen pseudomonas fuscovaginae contains two conserved quorum sensing systems involved in virulence and negatively regulated by rsal and the novel regulator rsam. | pseudomonas fuscovaginae is a gram-negative fluorescent pseudomonad pathogenic towards several plant species. despite its importance as a plant pathogen, no molecular studies of virulence have thus far been reported. in this study we show that p. fuscovaginae possesses two conserved n-acyl homoserine lactone (ahl) quorum sensing (qs) systems which we designated pfsi/r and pfvi/r. the pfsi/r system is homologous to the bvii/r system of burkholderia vietnamiensis and produces and responds to c10-h ... | 2011 | 20701623 |
| In Vitro Antibacterial Activity of Sphaeropsidins and Chemical Derivatives toward Xanthomonas oryzae pv. oryzae, the Causal Agent of Rice Bacterial Blight. | Sphaeropsidin A, the main phytotoxin produced by Diplodia cupressi, as well as the two natural analogues sphaeropsidins B and C and 14 derivatives obtained by chemical modifications were assayed for antibacterial activity against Xanthomonas oryzae pv. oryzae, Pseudomonas fuscovaginae, and Burkholderia glumae, the causal agents of severe bacterial rice diseases. The results showed a strong and specific activity of sphaeropsidin A against X. oryzae pv. oryzae, while no activity was observed again ... | 2011 | 22124378 |
| dissection of quorum-sensing genes in burkholderia glumae reveals non-canonical regulation and the new regulatory gene tofm for toxoflavin production. | burkholderia glumae causes bacterial panicle blight of rice and produces major virulence factors, including toxoflavin, under the control of the quorum-sensing (qs) system mediated by the luxi homolog, tofi, and the luxr homolog, tofr. in this study, a series of markerless deletion mutants of b. glumae for tofi and tofr were generated using the suicide vector system, pkksacb, for comprehensive characterization of the qs system of this pathogen. consistent with the previous studies by other resea ... | 2012 | 23284909 |
| rice sheath rot: an emerging ubiquitous destructive disease complex. | around one century ago, a rice disease characterized mainly by rotting of sheaths was reported in taiwan. the causal agent was identified as acrocylindrium oryzae, later known as sarocladium oryzae. since then it has become clear that various other organisms can cause similar disease symptoms, including fusarium sp. and fluorescent pseudomonads. these organisms have in common that they produce a range of phytotoxins that induce necrosis in plants. the same agents also cause grain discoloration, ... | 2015 | 26697031 |
| cyclic nucleotide-gated ion channel gene family in rice, identification, characterization and experimental analysis of expression response to plant hormones, biotic and abiotic stresses. | cyclic nucleotide-gated channels (cngcs) are ca2+-permeable cation transport channels, which are present in both animal and plant systems. they have been implicated in the uptake of both essential and toxic cations, ca2+ signaling, pathogen defense, and thermotolerance in plants. to date there has not been a genome-wide overview of the cngc gene family in any economically important crop, including rice (oryza sativa l.). there is an urgent need for a thorough genome-wide analysis and experimenta ... | 2014 | 25280591 |
| identification of virulence associated loci in the emerging broad host range plant pathogen pseudomonas fuscovaginae. | pseudomonas fuscovaginae (pfv) is an emerging plant pathogen of rice and also of other gramineae plants. it causes sheath brown rot disease in rice with symptoms that are characterized by brown lesions on the flag leaf sheath, grain discoloration and sterility. it was first isolated as a high altitude pathogen in japan and has since been reported in several countries throughout the world. pfv is a broad host range pathogen and very little is known about its virulence mechanisms. | 2014 | 25394860 |
| multiplex pcr assay for simultaneous detection of six major bacterial pathogens of rice. | the aim of this study was to develop a multiplex pcr (mpcr) assay for rapid, sensitive and simultaneous detection of six important rice pathogens: xanthomonas oryzae pv. oryzae, x. oryzae pv. oryzicola, pseudomonas fuscovaginae, burkholderia glumae, burkholderia gladioli and acidovorax avenae subsp. avenae. | 2016 | 26864896 |
| genome sequence of the rice pathogen pseudomonas fuscovaginae cb98818. | pseudomonas fuscovaginae is a phytopathogenic bacterium causing bacterial sheath brown rot of cereal crops. here, we present the draft genome sequence of p. fuscovaginae cb98818, originally isolated from a diseased rice plant in china. the draft genome will aid in epidemiological studies, comparative genomics, and quarantine of this broad-host-range pathogen. | 2012 | 22965098 |
| draft genome sequence of pseudomonas fuscovaginae, a broad-host-range pathogen of plants. | pseudomonas fuscovaginae was first reported as a pathogen of rice causing sheath rot in plants grown at high altitudes. p. fuscovaginae is now considered a broad-host-range plant pathogen causing disease in several economically important plants. we report what is, to our knowledge, the first draft genome sequence of a p. fuscovaginae strain. | 2012 | 22535942 |
| quorum sensing and rsam regulons of the rice pathogen pseudomonas fuscovaginae. | pseudomonas fuscovaginae (pfv) is an emerging plant pathogen causing sheath brown rot in rice, as well as diseases in other gramineae food crops including maize, sorghum and wheat. pfv possesses two conserved n-acyl homoserine lactone (ahl) quorum sensing (qs) systems called pfvi/r and pfsi/r, which are repressed by rsal and rsam, respectively. the two systems are not hierarchically organized and are involved in plant virulence. in this study the ahl qs pfsi/r, pfvi/r and rsam regulons were dete ... | 2017 | 28530166 |