| isolation and identification of an eps-producing rhizobium strain from arid soil (algeria): characterization of its eps and the effect of inoculation on wheat rhizosphere soil structure. | the production of exopolysaccharides (epss) by bacterial populations in the rhizosphere has been demonstrated to contribute to water and nutrient uptake by plant roots through the modification of the physical properties of rhizosphere soil. we report here the characterization of a new eps produced by a bacterial strain (kygt207) isolated from an arid soil in southern algeria (gassi touil), and the effect of inoculation of this strain on soil physical properties in the rhizosphere of triticum dur ... | 2005 | 15862451 |
| rhizobium alamii sp. nov., an exopolysaccharide-producing species isolated from legume and non-legume rhizospheres. | a group of exopolysaccharide-producing bacteria was isolated from the root environment of arabidopsis thaliana. the genetic diversity revealed by rep-pcr fingerprinting indicated that the isolates correspond to different strains. 16s rrna gene sequence analysis showed that the isolates are closely related to the strains rhizobium sp. yas34 and usda 1920, respectively isolated from sunflower roots and medicago ruthenica nodules. these bacteria belong to the rhizobium lineage of the alphaproteobac ... | 2009 | 19196780 |
| physiological responses and antioxidant enzyme changes in sulla coronaria inoculated by cadmium resistant bacteria. | plant growth promoting bacteria (pgpb) may help to reduce the toxicity of heavy metals on plants growing in polluted soils. in this work, sulla coronaria inoculated with four cd resistant bacteria (two pseudomonas spp. and two rhizobium sullae) were cultivated in hydroponic conditions treated by cd; long time treatment 50 µm cdcl2 for 30 days and short time treatment; 100 µm cdcl2 for 7 days. results showed that inoculation with cd resistant pgpb enhanced plant biomass, thus shoot and root dry w ... | 2017 | 28808815 |
| draft genome sequence of the nitrogen-fixing rhizobium sullae type strain is123(t) focusing on the key genes for symbiosis with its host hedysarum coronarium l. | the prominent feature of rhizobia is their molecular dialogue with plant hosts. such interaction is enabled by the presence of a series of symbiotic genes encoding for the synthesis and export of signals triggering organogenetic and physiological responses in the plant. the genome of the rhizobium sullae type strain is123(t) nodulating the legume hedysarum coronarium, was sequenced and resulted in 317 scaffolds for a total assembled size of 7,889,576 bp. its features were compared with those of ... | 2017 | 28798728 |
| rhizobium flavum sp. nov., a triazophos-degrading bacterium isolated from soil under the long-term application of triazophos. | a gram-stain-negative, non-motile, pale yellow, rod-shaped bacterial strain, yw14(t), was isolated from soil and its taxonomic position was investigated by a polyphasic study. strain yw14(t) did not form nodules on three different legumes, and the nodd and nifh genes were not detected by pcr. strain yw14(t) contained q-10 as the predominant ubiquinone. the major cellular fatty acid was c(18 : 1)ω7c. phylogenetic analyses based on 16s rrna gene sequences and seven housekeeping gene sequences (rec ... | 2014 | 24639241 |
| phenotypic and genetic characterization of rhizobia isolated from hedysarum flexuosum in northwest region of morocco. | seventy bacterial strains were isolated from root nodules of the legume hedysarum flexuosum grown wild in soils from northwest morocco. repetitive extragenic palindromic (rep)-polymerase chain reaction (pcr) clustered the strains into 30 rep-pcr groups. the nearly complete sequence of the 16s rrna gene from a representative strain of each rep-pcr pattern showed that 17 strains were closely related to members of the genus rhizobium of the family rhizobiaceae of the alphaproteobacteria. pairwise a ... | 2015 | 25721451 |
| molecular phylogenetic analysis of rhizobium sullae isolated from algerian hedysarum flexuosum. | isolates from root nodules of hedysarum flexuosum, sampled from north region of algeria, were analyzed on the basis of their phenotypic and molecular characteristics. they were tested for their tolerance to nacl, ph, temperatures, antibiotics and heavy metals resistance. interestingly, the isolate hf_04n appeared resistant to zncl2 (50 μg/ml) and grew at high saline concentration up to 9 %. the phylogenetic positions of five isolates were studied by comparative sequence analysis of 16s rrna, rec ... | 2016 | 27034287 |
| characterization of efficient plant-growth-promoting bacteria isolated from sulla coronaria resistant to cadmium and to other heavy metals. | the inoculation of plants with plant-growth-promoting rhizobacteria has become a priority in the phytoremediation of heavy-metal-contaminated soils. a total of 82 bacteria were isolated from sulla coronaria root nodules cultivated on four soil samples differently contaminated by heavy metals. the phenotypic characterization of these isolates demonstrated an increased tolerance to cadmium reaching 4.1mm, and to other metals, including zn, cu and ni. polymerase chain reaction/restriction fragment ... | 2017 | 27498183 |
| rhizobium hedysari sp. nov., a novel species isolated from a root nodule of hedysarum multijugum in china. | a strain 5-1-2(t) was isolated from a root nodule of hedysarum multijugum collected from zhangye city, gansu province, north-west china. phylogenetic analysis based on the 16s rrna gene sequence and other housekeeping genes (reca and atpd) indicated that the strain represents a novel species in the genus rhizobium close to the strain rhizobium subbaraonis jc85(t) with similarities of 98.27, 88.92 and 89.62%, respectively. strain 5-1-2(t) contained q-10 as the predominant ubiquinone. our results ... | 2017 | 28097539 |
| specificity in legume-rhizobia symbioses. | most species in the leguminosae (legume family) can fix atmospheric nitrogen (n₂) via symbiotic bacteria (rhizobia) in root nodules. here, the literature on legume-rhizobia symbioses in field soils was reviewed and genotypically characterised rhizobia related to the taxonomy of the legumes from which they were isolated. the leguminosae was divided into three sub-families, the caesalpinioideae, mimosoideae and papilionoideae. bradyrhizobium spp. were the exclusive rhizobial symbionts of species i ... | 2017 | 28346361 |
| rhizobium halotolerans sp. nov., isolated from chloroethylenes contaminated soil. | the strain designated as ab21(t) was isolated from chloroethylenes contaminated soil. cells are gram-negative, aerobic, non-spore-forming, and motile rods. phylogenetic analysis based on 16s rrna gene sequence showed that it belonged to the genus rhizobium, and was closely related to rhizobium sullae is 123(t) (97.4 %), rhizobium yanglingense sh 22623(t) (97.2 %), rhizobium gallicum r 602sp(t) (97.1 %), rhizobium alamii gbv 016(t) (97.0 %), and rhizobium monogolense usda 1844(t) (97.0 %). it sho ... | 2013 | 23377488 |
| high-quality permanent draft genome sequence of rhizobium sullae strain wsm1592; a hedysarum coronarium microsymbiont from sassari, italy. | rhizobium sullae strain wsm1592 is an aerobic, gram-negative, non-spore-forming rod that was isolated from an effective nitrogen (n2) fixing root nodule formed on the short-lived perennial legume hedysarum coronarium (also known as sulla coronaria or sulla). wsm1592 was isolated from a nodule recovered from h. coronarium roots located in ottava, bordering sassari, sardinia in 1995. wsm1592 is highly effective at fixing nitrogen with h. coronarium, and is currently the commercial sulla inoculant ... | 2015 | 26380632 |
| relevance of fucose-rich extracellular polysaccharides produced by rhizobium sullae strains nodulating hedysarum coronarium l. legumes. | specific and complex interactions between soil bacteria, known as rhizobia, and their leguminous host plants result in the development of root nodules. this process implies a complex dialogue between the partners. rhizobia synthesize different classes of polysaccharides: exopolysaccharides (eps), kdo-rich capsular polysaccharides, lipopolysaccharides, and cyclic β-(1,2)-glucans. these polymers are actors of a successful symbiosis with legumes. we focus here on studying the eps produced by rhizob ... | 2013 | 23183977 |
| nodulation-gene-inducing flavonoids increase overall production of autoinducers and expression of n-acyl homoserine lactone synthesis genes in rhizobia. | legume-nodulating rhizobia use n-acyl homoserine lactones (ahls) to regulate several physiological traits related to the symbiotic plant-microbe interaction. in this work, we show that sinorhizobium fredii smh12, rhizobium etli isp42 and rhizobium sullae is123, three rhizobial strains with different nodulation ranges, produced a similar pattern of ahl molecules, sharing, in all cases, production of n-octanoyl homoserine lactone and its 3-oxo and/or 3-hydroxy derivatives. interestingly, productio ... | 2011 | 21600980 |
| isolation and partial characterization of bacterial strains on low organic carbon medium from soils fertilized with different organic amendments. | a total of 720 bacterial strains were isolated from soils with four different organic amendment regimes on a low organic carbon (low-c) agar medium (10 µg c ml(-1)) traditionally used for isolation of oligotrophs. organic amendments in combination with field history resulted in differences in dissolved organic carbon contents in these soils. there were negative correlations between total and dissolved organic carbon content and the number of isolates on low-c agar medium, whereas these correlati ... | 2010 | 20422409 |
| rhizobium mesosinicum sp. nov., isolated from root nodules of three different legumes. | thirteen novel strains were isolated from root nodules of three different leguminous plants of the genera albizia, kummerowia and dalbergia grown in china. cells of these strains were gram-negative, strictly aerobic, non-spore-forming, motile rods. phylogenetic analysis of 16s rrna gene sequences revealed that they belong to the genus rhizobium. a representative strain, ccbau 25010t, showed 98.8% 16s rrna gene sequence similarity to its closest phylogenetic relative, rhizobium sullae is123t. the ... | 2009 | 19567587 |
| selenite-reducing capacity of the copper-containing nitrite reductase of rhizobium sullae. | rhizobium sullae strain hcnt1 contains a nitric oxide-producing nitrite reductase of unknown function due to the absence of a complementary nitric oxide reductase. hcnt1 had the ability to grow on selenite concentrations as high as 50 mm, and during growth, selenite was reduced to the less toxic elemental selenium. an hcnt1 mutant lacking nitrite reductase grew poorly in the presence of 5 mm selenite, was unable to grow in the presence of 25 or 50 mm selenite and also showed no evidence of selen ... | 2007 | 17227457 |
| investigation into the role of the truncated denitrification chain in rhizobium sullae strain hcnt1. | most denitrifying bacteria reduce nitrate to the inert gases nitrous oxide or nitrogen. a remarkable exception to this is rhizobium sullae strain hcnt1, which catalyses only a single step in the denitrification pathway, the reduction of nitrite to the reactive molecule nitric oxide. further study demonstrated that hcnt1 does not encode the genes for no reductase. prolonged incubation of hcnt1 under anoxic conditions revealed that the cells had reduced culturability but not viability when nitrite ... | 2006 | 16417501 |
| pcr primers based on different portions of insertion elements can assist genetic relatedness studies, strain fingerprinting and species identification in rhizobia. | using the sequence of an insertion element originally found in rhizobium sullae, the nitrogen-fixing bacterial symbiont of the legume hedysarum coronarium, we devised three primer pairs (inbound, outbound and internal primers) for the following applications: (a) tracing genetic relatedness within rhizobia using a method independent of ribosomal inheritance, based on the presence and conservation of is elements; (b) achieve sensitive and reproducible bacterial fingerprinting; (c) enable a fast an ... | 2005 | 16332341 |
| gamma proteobacteria can nodulate legumes of the genus hedysarum. | the bacteria hosted in the root nodules of the three mediterranean wild legume species hedysarum carnosum, hedysarum spinosissimum subsp. capitatum, and hedysarum pallidum, growing in native stands in different habitats in algeria were isolated. bacteria were recovered on yeast-mannitol-agar or on minimal media from a total of 52 nodules. isolates were analyzed by amplified ribosomal dna restriction analysis (ardra) using the enzyme cfoi, and further sorted by rapd fingerprinting. a total of ten ... | 2004 | 15368852 |
| rhizobium sullae sp. nov. (formerly 'rhizobium hedysari'), the root-nodule microsymbiont of hedysarum coronarium l. | this work is the completion of a series of reports describing the nitrogen-fixing bacterial symbionts of sulla (hedysarum coronarium l., leguminosae) and providing the grounds for their proposal as a new taxon. the introduction summarizes a large amount of previous evidence gathered on the physiology, genetics and ecology of such organisms, which have in the past been referred to provisionally as 'rhizobium hedysari'. upon adding 16s rna sequencing, amplified rdna restriction analysis of the rrn ... | 2002 | 12148639 |
| characterization of the gene encoding nitrite reductase and the physiological consequences of its expression in the nondenitrifying rhizobium "hedysari" strain hcnt1. | rhizobium "hedysari" hcnt1 is an unclassified rhizobium which contains a nitric oxide-producing nitrite reductase but is apparently incapable of coupling the reduction of nitrite to energy conservation. the gene encoding the nitrite reductase, nirk, has been cloned and sequenced and was found to encode a protein closely related to the copper-containing family of nitrite reductases. unlike other members of this family, nirk expression in hcnt1 is not dependent on the presence of nitrogen oxides, ... | 1996 | 8899992 |