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multilocus sequence typing confirms the close genetic interrelatedness of three distinct flavescence dorée phytoplasma strain clusters and group 16srv phytoplasmas infecting grapevine and alder in europe.vineyards of southern france and northern italy are affected by the flavescence dorée (fd) phytoplasma, a quarantine pathogen transmitted by the leafhopper of nearctic origin scaphoideus titanus. to better trace propagation of fd strains and identify possible passage between the vineyard and wild plant compartments, molecular typing of phytoplasma strains was applied. the sequences of the two genetic loci map and uvrb-degv, along with the sequence of the secy gene, were determined among a collec ...200717468266
'candidatus phytoplasma spartii', 'candidatus phytoplasma rhamni' and 'candidatus phytoplasma allocasuarinae', respectively associated with spartium witches'-broom, buckthorn witches'-broom and allocasuarina yellows diseases.spartium witches'-broom (spawb), buckthorn witches'-broom (bwb) and allocasuarina yellows (alloy) are witches'-broom and yellows diseases of spartium junceum (spanish broom), rhamnus catharticus (buckthorn) and allocasuarina muelleriana (slaty she-oak), respectively. these diseases are associated with distinct phytoplasmas. the spawb, bwb and alloy phytoplasmas share <97.5 % 16s rdna sequence similarity with each other and with other known phytoplasmas, including the closely related phytoplasmas ...200415280265
bradyrhizobium sp. nodulating the mediterranean shrub spanish broom (spartium junceum l.).the molecular diversity of 25 strains of rhizobia, isolated in sicily from root nodules of the mediterranean shrubby legume spanish broom (spartium junceum l.), is presented in relation to the known rhizobial reference strains.200211849323
purification and characterization of two types of cytisus multiflorus hemagglutinin by affinity chromatography.two hemagglutinins were separated from extracts of cytisus multiflorus seeds by successive affinity chromatographies on columns of galactose- and di- n-acetylchitobiose-sepharose 4b. one was found to be inhibited by di- n-acetylchitobiose or tri- n-acetylchitotriose and shown to possess anti-h(o) activity [cytisus-type anti-h(o) hemagglutinin designated as cytisus multiflorus hemagglutinin i]. the other, which was not a blood group-specific hemagglutinin, was inhibited by galactose or lactose (h ...19836663442
sugar binding specificities of anti-h(o) lectins disclosed by use of fucose-containing human milk oligosaccharides as binding inhibitors.the binding to normal and sialidase-treated human erythrocytes of six 125i-labeled lectins [ulex europeus lectin i (uea-1) and ii (uea-ii), laburnum alpinum lectins i (laa-i) and ii (laa-ii), and cytisus multiflorus lectins i (cma-i) and ii (cma-ii)], was studied in detail. quantitative inhibition assays of the lectin binding to the cells were also performed with various human milk oligosaccharides as inhibitors. based on a comparison of the inhibition constants of the inhibitors thus obtained w ...19892752484
phylogenetic relationships of xylella fastidiosa strains isolated from landscape ornamentals in southern california.abstract xylella fastidiosa is an insect-borne, xylem-limited pathogenic bacterium that has been associated with a rise in incidence of diseased landscape ornamentals in southern california. the objective of this study was to genetically characterize strains isolated from ornamental hosts to understand their distribution and identity. strains of x. fastidiosa isolated from ornamentals were characterized using a multiprimer polymerase chain reaction (pcr) system, random amplified polymorphic dna ...200718943935
transfer of 238u, 230th, 226ra, and 210pb from soils to tree and shrub species in a mediterranean area.the soil-to-plant transfer factors of natural uranium isotopes ((238)u and (234)u), (230)th, (226)ra, and (210)pb were studied in a disused uranium mine located in the extremadura region in the south-west of spain. the plant samples included trees (quercus ilex, quercus suber, and eucalyptus cameldulensis) and one shrub (cytisus multiflorus). all of them are characteristic of mediterranean environments. the activity concentrations in leaves and fruit were determined for the tree species at diffe ...201020153657
flash pyrolysis of forestry residues from the portuguese central inland region within the framework of the biorefina-ter project.the feasibility of the valorization by flash pyrolysis of forest shrub wastes, namely bushes (cytisus multiflorus, spartium junceum, acacia dealbata and pterospartum tridentatum) has been studied in a conical spouted bed reactor operating at 500 °c, with a continuous biomass feed and char removal. high bio-oil yields in the 75-80 wt.% range have been obtained for all of the materials, with char yields between 16 and 23 wt.% and low gas yields (4-5 wt.%). bio-oils are composed mainly of water (ac ...201323266853
a new physical-chemical process for the efficient production of cellulose fibers from spanish broom (spartium junceum l.).a novel and efficient method for the extraction of cellulose fibers from spanish broom (spartium junceum l.) is presented. the method is based on the sequential combination between an initial chemical stage (alkaline digestion) and a subsequent physical-chemical stage, consisting of compression with hot air in an autoclave followed by rapid decompression (dicode process, digestion-compression-decompression). the alkaline mother liquor deriving from the initial digestion step can be conveniently ...201019734042
self-sterility in two cytisus species (leguminosae, papilionoideae) due to early-acting inbreeding depression.in most angiosperms, the endosperm develops before the embryo, but with harmony between the two structures until final seed formation. in an embryological study, we show that inbreeding depression causes disharmony in development of the two structures in two leguminosae shrubs, cytisus multiflorus and c. striatus. our main objective was to test the causes of self-sterility in the two species by comparing the embryological development of the self seeds with that of cross seeds. in developing self ...201021622373
influence of the drying method in the antioxidant potential and chemical composition of four shrubby flowering plants from the tribe genisteae (fabaceae).flowers from several common mediterranean shrubs, such as those from the cytisus genus and genista genus (tribe genisteae/fabaceae) have long been used for medicinal purposes and seasoning in the northeastern portuguese region. despite, the shade-drying traditionally used to process these plants, freeze-drying is claimed to better preserve the quality of medicinal plants. herein, the effects of drying process in the antioxidants composition and properties of cytisus multiflorus, cytisus scopariu ...201121816201
spartium junceum aromatic water: chemical composition and antitumor activity.the purpose of this study was to analyse the chemical composition of spartium junceum l. (also known as spanish broom) aromatic water and to evaluate its cytotoxic activity against a series of human cancer cell lines (melanoma: rpmi 7932; leukemia: k562; breast cancer cell: mcf7-bart and mcf7-iclc, colon adenocarcinoma: sw480). the results show that the aromatic water was cytotoxic toward the tumor cell lines analyzed (rpmi 7932, k562, mcf7-bart, mcf7-iclc, sw480), while it did not appreciably a ...201222428268
[spartium junceum (spanish broom) poisoning]. 201424582126
metal uptake by native plants and revegetation potential of mining sulfide-rich waste-dumps.waste dumps resulting from metal exploitation create serious environmental damage, providing soil and water degradation over long distances. phytostabilization can be used to remediate these mining sites. the present study aims to evaluate the behavior of selected plant species (erica arborea, ulex europaeus, agrostis delicatula, and cytisus multiflorus) that grow spontaneously in three sulfide-rich waste-dumps (lapa grande, cerdeirinha, and penedono, portugal). these sites represent different g ...201424933904
purification and structural stability of white spanish broom (cytisus multiflorus) peroxidase.new plant peroxidase has been isolated to homogeneity from the white spanish broom cytisus multiflorus. the enzyme purification steps included homogenization, nh(4)so(4) precipitation, extraction of broom colored compounds and consecutive chromatography on phenyl-sepharose, hitrap™ sp hp and superdex-75 and 200. the novel peroxidase was characterized as having a molecular weight of 50 ± 3 kda. steady-state tryptophan fluorescence and far-uv circular dichroism (cd) studies, together with enzymati ...201525246165
snowpack, fire, and forest disturbance: interactions affect montane invasions by non-native shrubs.montane regions worldwide have experienced relatively low plant invasion rates, a trend attributed to increased climatic severity, low rates of disturbance, and reduced propagule pressure relative to lowlands. manipulative experiments at elevations above the invasive range of non-native species can clarify the relative contributions of these mechanisms to montane invasion resistance, yet such experiments are rare. furthermore, global climate change and land use changes are expected to cause decr ...201525482316
mechanism-based suicide inactivation of white spanish broom (cytisus multiflorus) peroxidase by excess hydrogen peroxide.suicide inactivation is a common mechanism observed for haem peroxidases, in which the enzyme is inactivated as a result of self-oxidation mediated by intermediate highly oxidizing enzyme forms during the catalytic cycle. the time-dependence and the inactivation mechanism of cytisus multiflorus peroxidase (cmp) by hydrogen peroxide were studied kinetically with four co-substrates (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (abts), ferulic acid, guaiacol and o-dianisidine). catalytic ...201526407901
kinetics of spanish broom peroxidase obeys a ping-pong bi-bi mechanism with competitive inhibition by substrates.in plants, adverse conditions often induce an increase in reactive oxygen species (ros) such as hydrogen peroxide (h2o2). h2o2 is reduced to water, and thus becomes detoxified by enzymes such as cytisus multiflorus peroxidase (cmp). here, the steady-state kinetics of the h2o2-supported oxidation of different organic substrates by cmp was investigated. analysis of the initial rates vs. h2o2 and reducing substrate concentrations proved to be consistent with a substrate-inhibited ping-pong bi-bi re ...201526416239
study of the adsorption of mercury (ii) on lignocellulosic materials under static and dynamic conditions.who has declared mercury as one of the most dangerous pollutants for human health. unfortunately, several cases of rivers and aquifers contaminated by mercury inevitably poses the problem on how to remediate them. considerable efforts are being addressed to develop cost-effective methodologies, among which the use of low-cost adsorbing materials. in this paper, the adsorption performances of an alternative lignocellulosic material derived from the spanish broom plant, are presented. this plant i ...201728390230
the response of spartium junceum roots to slope: anchorage and gene factors.plant anchorage is governed by complex, finely regulated mechanisms that occur at a morphological, architectural and anatomical level. spanish broom (spartium junceum) is a woody plant frequently found on slopes--a condition that affects plant anchorage. this plant grows throughout the mediterranean area where it plays an important role in preventing landslides. spanish broom seedlings respond promptly to slope by altering stem and root morphology. the aim of this study was to investigate the me ...200616352708
megasporogenesis, megagametogenesis and ontogeny of the aril in cytisus striatus and c. multiflorus (leguminosae: papilionoideae).there are few embryological reports on wild legumes and even fewer on their seminal appendages. there are no existing studies on the complete ontogeny of these appendages in cytiseae, a very important papilionoideae tribe in mediterranean ecosystems. in this work megasporogenesis, megagametogenesis and aril ontogeny were studied in cytisus multiflorus and c. striatus, endemics from the western mediterranean region.200616873423
spanish broom flower ingestion: a very unusual poisoning. 200616969244
characterization of phenolic compounds in flowers of wild medicinal plants from northeastern portugal.crataegus monogyna, cytisus multiflorus, malva sylvestris and sambucus nigra have been used as important medicinal plants in the iberian peninsula since a long time ago, and are claimed to have various health benefits. this study aimed to determine the phenolic profile and composition of wild medicinal flowers of those species. the analysis was performed by hplc-dad-esi/ms. flavonoids, and particularly flavonols and flavones, were the main groups in almost all the studied samples. c. multiflorus ...201222342808
bioactive compounds, rp-hplc analysis of phenolics, and antioxidant activity of some portuguese shrub species extracts.in the ecosystem of serra da estrela, some plant species have the potential to be used as raw material for extraction of bioactive products. the goal of this work was to determine the phenolic, flavonoid, tannin and alkaloid contents of the methanolic extracts of some shrubs (echinospartum ibericum, pterospartum tridentatum, juniperus communis, ruscus aculeatus, rubus ulmifolius, hakea sericea, cytisus multiflorus, crataegus monogyna, erica arborea and ipomoea acuminata), and then to correlate t ...201122312726
spanish broom (spartium junceum l.) fibers impregnated with vancomycin-loaded chitosan nanoparticles as new antibacterial wound dressing: preparation, characterization and antibacterial activity.in this work, we propose as new wound dressing, the spanish broom fibers impregnated with vancomycin (vm) loaded chitosan nanoparticles. spanish broom fibers were extracted by patented method dicode and the morphological, physical and mechanical properties were investigated. chitosan nanoparticles were prepared by ionic gelation using different weight ratios between chitosan (ch) and tripolyphosphate (tpp). nanoparticles were characterized in terms of size, zeta potential, yield, encapsulation e ...201727931851
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