| [cloning and expression of alpha-d-galactosidase from coffee bean (coffea liberica & coffea canephora)]. | alpha-d-galactosidase (alpha-gal,e.c. 3.2.1.22) is an exo-glycosidase. the enzyme isolated from coffee beans has been well characterized. it has high activity in hydrolyzing the terminal alpha-d-galactoside residues from glycoconjugates on human blood group b erythrocytes, as well as in converting the blood group b into o. a different 1089 bp cdna open reading frame(orf) encoding gal of coffea liberica & c. canephora was cloned by homology-based rt-pcr. the cloned gal most closely resembles the ... | 2005 | 16257905 |
| antioxidant potential, tannin and polyphenol contents of seed and pericarp of three coffea species. | to investigate the antioxidant activity, total phenolic and total tannin content of the pericarp and the seed of coffea benghalensis (c. benghalensis) and coffea liberica compared to coffea arabica (c. arabica). | 2016 | 27086155 |
| ssr cross-amplification and variation within coffee trees (coffea spp.). | primer sets were developed from 85 coffea arabica sequences in addition to 25 already published primer sets. they were subsequently used for amplification in six african coffea species: coffea canephora (can), coffea eugenioides (eug), coffea heterocalyx (het), coffea liberica (lib), coffea sp. moloundou (mol) and coffea pseudozanguebariae (pse). the amplification percentages for these 110 primer pairs ranged from 72.7% for lib to 86.4% for pse. good transferability was thus obtained within the ... | 2004 | 15644965 |
| metabolic relations between methylxanthines and methyluric acids in coffea l. | metabolism of purine alkaloids in the leaves of coffea dewevrei de wild et durand var excelsa chev, coffea liberica bull ex hiern and coffea abeokutae cramer was studied by analyzing leaf discs collected during vegetative development and by feeding the following radioactive tracers: [(14)c]theobromine, [(14)c]caffeine, and [(14)c]theacrine (1,3,7,9-tetramethyluric acid). their principal metabolites were quantitatively and qualitatively determined. all three species convert the precursors to the ... | 1983 | 16663351 |
| reliable flow cytometric estimation of nuclear dna content in coffee trees. | flow cytometry gave high resolution of genome size in two coffee taxa (coffea liberica dewevrei and c. pseudozanguebariae). propidium iodide (pi) and petunia hybrida were used as dye and internal standard, respectively. proportionally between the dna content and the digitized fluorescence signal was checked. five main results were evident. first, optimal experimental conditions were established for peak location estimation (mean or mode), staining time (at least 2 minutes), high voltage (557 v) ... | 1996 | 8723900 |
| effect of the roasting method on the content of 5-hydroxytryptamides of carboxylic acids in roasted coffee beans. | coffee beans of coffea liberica (robusta) variety were roasted using convection and microwave heating. for roasting we used green coffee beans of 7.5% moisture content, and beans wetted to 10% moisture content and dried to 5% moisture content. the content of 5-hydroxytryptamides of carboxylic acids c-5-ht (determined by tlc) as the index of substances irritating alimentary canal was investigated in the roasted beans, depending on the bean treatment before roasting and applied roasting method. an ... | 2002 | 12224425 |
| consequences of stoichiometric error on nuclear dna content evaluation in coffea liberica var. dewevrei using dapi and propidium iodide. | the genome size of coffee trees (coffea sp.) was assessed using flow cytometry. nuclear dna was stained with two dyes [4',6-diamino-2-phenylindole dihydrochloride hydrate (dapi) and propidium iodide (pi)]. fluorescence in coffee tree nuclei (c-pi or c-dapi) was compared with that of the standard, petunia (p-pi or p-dapi). if there is no stoichiometric error, then the ratio between fluorescence of the target nuclei and that of the standard nuclei (r-pi or r-dapi) is expected to be proportional to ... | 2002 | 12096798 |
| aflp and ssr polymorphism in a coffea interspecific backcross progeny [( c. heterocalyx x c. canephora) x c. canephora]. | an interspecific cross (bc 1) involving a species with one of the largest genomes in the coffea genus [ coffea heterocalyx (het), qdna = 1.74 pg] and a species with a medium-sized genome [ coffea canephora (can), qdna = 1.43 pg] was studied using two types of molecular markers, aflp and ssr. one hundred and eighty eight aflp bands and 34 ssr primer pairs were suitable for mapping. the total map length was 1,360 cm with 190 loci distributed in 15 linkage groups. the results were compared to those ... | 2003 | 12879256 |
| aflp analysis of genetic diversity within and among coffea arabica cultivars. | genetic diversity of coffea arabica cultivars was estimated using amplified fragment length polymorphism (aflp) markers. sixty one coffea accessions composed of six arabica cultivars, including typica, bourbon, catimor, catuai, caturra and mokka hybrid, plus two diploid coffea species, were analyzed with six ecori- msei primer combinations. a total of 274 informative aflp markers were generated and scored as binary data. these data were analyzed using cluster methods in the software package ntsy ... | 2002 | 12582521 |
| genetic mapping of a caffeoyl-coenzyme a 3-o-methyltransferase gene in coffee trees. impact on chlorogenic acid content. | chlorogenic acids (cga) are involved in the bitterness of coffee due to their decomposition in phenolic compounds during roasting. cga mainly include caffeoyl-quinic acids (cqa), dicaffeoyl-quinic acids (dicqa) and feruloyl-quinic acids (fqa), while cqa and dicqa constitute cga sensu stricto (cga s.s.). in the two cultivated species coffea canephora and coffea arabica, cga s.s. represents 88% and 95% of total cga, respectively. among all enzymes involved in cga biosynthesis, caffeoyl-coenzyme a ... | 2003 | 12861362 |
| fish using a gag-like fragment probe reveals a common ty3-gypsy-like retrotransposon in genome of coffea species. | the genus coffea possesses about 100 species, and the most economically important are coffea canephora and coffea arabica. the latter is predominantly self-compatible with 2n = 4x = 44, while the others of the genus are diploid with 2n = 2x = 22 and mostly self-incompatible. studies using molecular markers have been useful to detect differences between genomes in coffea; however, molecular and cytogenetic studies have produced only limited information on the karyotypes organization. we used dop- ... | 2012 | 23231601 |
| phytochemical overview and medicinal importance of coffea species from the past until now. | coffea (coffee) species are grown in almost all countries along the equator. many members of the genus have a large production history and an important role both in the global market and researches. seeds (coffeae semen) are successfully used in food, cosmetic, and pharmaceutical industries due to its caffeine and high polyphenol content. nowadays, the three best-known coffee species are arabic (coffea arabica l.), robusta (coffea robusta l. linden), and liberian coffees (coffea liberica hiern.) ... | 2016 | 27955739 |
| survey of philippine coffee beans for the presence of ochratoxigenic fungi. | in 2012 to 2014, philippine green coffee beans from coffea arabica in benguet and ifugao; coffea canephora var. robusta in abra, cavite, and ifugao; and coffea liberica and coffea excelsea from cavite were collected and assessed for the distribution of fungi with the potential to produce ochratoxin a (ota). the presence of fungal species was evaluated both before and after surface sterilization. there were remarkable ecological and varietal differences in the population of ota-producing species ... | 2016 | 26814977 |
| introgression molecular analysis of a leaf rust resistance gene from coffea liberica into c. arabica l. | leaf rust caused by the fungus hemileia vastatrix is the most devastating disease of arabica coffee ( coffea arabica). therefore, developing leaf rust-resistant varieties has been a breeding objective of the highest priority in many countries. the purpose of the present work was to gain insight into the mechanism of introgression into c. arabica of a leaf rust resistance gene from c. liberica (i.e. s(h)3 resistance factor) and to identify associated molecular markers. an f(2) progeny (i.e. 101 i ... | 2004 | 15241596 |
| [coffea arabica--production, botanical classification, new components and active agents and their pharmacological effects]. | the present paper surveys the latest knowledge about coffee grains (coffea arabica) as an important part of our everyday life. it lists the botanical classification of its principal species, such as coffea arabica, coffea robusta, and coffea liberica, the most important improved cultivars of production species in the world. the review paper also discusses the economic aspect, lists the most important regions of production, the optimal and farming technological conditions. it points out the occur ... | 2000 | 10953453 |