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regulation of defense and cryoprotective proteins by high levels of co(2) in annona fruit stored at chilling temperature.this study focuses on how the length of exposure to chilling temperature and atmosphere storage conditions regulate the hydrolytic activity and expression of chitinase (pr-q) and 1,3-beta-glucanase (pr-2) isoenzymes in cherimoyas (annona cherimola mill.). storage at 6 degrees c modified the expression of constitutive isoenzymes and induced the appearance of novel acidic chitinases, achi26 and achi24, at the onset of the storage period, and of a basic chitinase, bchi33, after prolonged storage. t ...200918538447
anti-helicobacter pylori activity of plants used in mexican traditional medicine for gastrointestinal disorders.helicobacter pylori is the major etiological agent of chronic active gastritis and peptic ulcer disease and is linked to gastric carcinoma. treatment to eradicate the bacteria failed in many cases, mainly due to antibiotic resistance, hence the necessity of developing better therapeutic regimens. mexico has an enormous unexplored potential of medicinal plants. this work evaluates the in vitro anti-h. pylori activity of 53 plants used in mexican traditional medicine for gastrointestinal disorders ...200919162157
analysis of the effect of high hydrostatic pressure treatment and enterocin as-48 addition on the bacterial communities of cherimoya pulp.in the present study, pulp obtained from cherimoya pulp (annona cherimola) was inoculated with epiphytic microbiota collected from cherimoya fruits, and supplemented or not with the circular bacteriocin enterocin as-48 (50μg/g) and then packed under vacuum. samples supplemented or not with enterocin were treated by high hydrostatic pressure (600mpa, 8min) and then stored at 5°c for 30days. the single as-48 treatment only delayed microbial growth non-significantly (p>0.05). hhp treatment reduced ...201525528726
a cryoprotective and cold-adapted 1,3-β-endoglucanase from cherimoya (annona cherimola) fruit.a 1,3-β-glucanase with potent cryoprotective activity was purified to homogeneity from the mesocarp of co(2)-treated cherimoya fruit (annona cherimola mill.) stored at low temperature using anion exchange and chromatofocusing chromatography. this protein was characterized as a glycosylated endo-1,3-β-glucanase with a m(r) of 22.07kda and a pi of 5.25. the hydrolase was active and stable in a broad acidic ph range and it exhibited maximum activity at ph 5.0. it had a low optimum temperature of 35 ...201121497868
annona squamosa seed extract in the regulation of hyperthyroidism and lipid-peroxidation in mice: possible involvement of quercetin.annona squamosa (custard apple) seeds are generally thrown away as waste materials. the extract of these seeds was evaluated for its possible ameliorative effect in the regulation of hyperthyroidism in mouse model. serum triiodothyronine (t(3)), thyroxine (t(4)) concentrations, hepatic glucose-6-phospatase (g-6-pase) and 5'-mono-deiodinase (5'di) activity were considered as the end parameters of thyroid function. simultaneously hepatic lipid peroxidation (lpo), superoxide dismutase (sod) and cat ...200717291737
two cyclopeptides from the seeds of annona cherimola.bioassay-guided fractionation of cytotoxic of methanol extract of the seeds of annona cherimola provided two novel cyclic peptides, cherimolacyclopeptide e (1) and cherimolacyclopeptide f (2), which exhibited significant cytotoxic activity against the kb (human nasopharyngeal carcinoma) cell culture system. the peptide 1 and 2 were elucidated by ms/ms fragmentation experiments using a q-tof mass spectrometer equipped with an esi source, extensive 2d nmr analyses and chemical degradation.200516040066
attraction of pollinators to atemoya (annona squamosa × annona cherimola) in puerto rico using commercial lures and food attractants.atemoya is a hybrid between annona squamosa l. and annona cherimola miller (annonaceae) and has potential to be an important fruit crop in tropical and subtropical areas. a major impediment to fruit production is low fruit set due to inadequate pollinator visits, typically, by beetles in the family nitidulidae. we used universal moth traps to monitor the attractiveness of two commercially available nitidulidae lures in combination with various food attractants, including raw bread dough, apple j ...201526470336
antidiabetic activity of aqueous leaf extract of annona squamosa in streptozotocin-nicotinamide type 2 diabetic rats.the claim by some tribal populations in parts of northern india that the young leaves of the custard apple tree, annona squamosa l. (annonaceae) has antidiabetic properties was investigated. diabetes mellitus was induced with streptozotocin-nicotinamide and graded doses of the aqueous leaf extracts were then administered in drinking water to normal and experimental diabetic rats for 12 days. fasting plasma glucose levels, serum insulin levels, serum lipid profiles and changes in body weight were ...200415036485
native and introduced host plants of anastrepha fraterculus and ceratitis capitata (diptera: tephritidae) in northwestern argentina.wild or commercially grown, native and exotic fruit were collected in 30 localities in the tucumán province (nw argentina) from january 1990 to december 1995 to determine their status as hosts of anastrepha fraterculus (wiedemann) and/or ceratitis capitata (wiedemann), the only two fruit fly species of economic and quarantine importance in argentina. a total of 84,094 fruit (3,466.1 kg) representing 33 species (7 native and 26 exotic) in 15 plant families were sampled. we determined the followin ...200314503581
modifications of allergenicity linked to food technologies.the prevalence of food allergies (fa) has increased over the past fifteen years. the reasons suggested are changes in dietary behaviour and the evolution of food technologies. new cases of fa have been described with chayote, rambutan, arguta, pumpkin seeds, custard apple, and with mycoproteins from fusarium.... additives using food proteins are at high risk: caseinates, lysozyme, cochineal red, papaïn, alpha-amylase, lactase etc. heating can reduce allergenicity or create neo-allergens, as well ...19989503097
an actidione resistant candida tropicalis from custard apple juice.an actidione resistant yeast, candida tropicalis, was isolated from fermenting custard apple juice. though a slight inhibition of growth was observed on the first day with 5000 ppm of actidione, growth was equal to control after one week. sorbic acid at 500 ppm and above inhibited the growth of this yeast while sodium benzoate and potassium metabisulphite were unable to suppress the growth even at 1000 ppm. fermentation and assimilation of different carbon sources were delayed in the presence of ...19817259152
the unusual acid-accumulating behavior during ripening of cherimoya (annona cherimola mill.) is linked to changes in transcription and enzyme activity related to citric and malic acid metabolism.cherimoya (annona cherimola mill.) is a subtropical fruit characterized by a significant increase in organic acid levels during ripening, making it an interesting model for studying the relationship between acidity and fruit flavor. in this work, we focused on understanding the balance between the concentration of organic acids and the gene expression and activity of enzymes involved in the synthesis and degradation of these metabolites during the development and ripening of cherimoya cv. "conch ...201627120592
profiling of lipophilic and phenolic phytochemicals of four cultivars from cherimoya (annona cherimola mill.).the lipophilic and phenolic extractives of the ripe mesocarp of four cherimoya cultivars ('perry vidal', 'mateus i', 'mateus iii' and 'funchal') from madeira island, were studied for the first time. the predominant lipophilic compounds are kaurene diterpenes (42.2-59.6%), fatty acids (18.0-35.6%) and sterols (9.6-23.7%). kaur-16-en-19-oic acid is the major lipophilic component of all cultivars accounting between 554 and 1350mgkg(-1) of dry material. the studied fruits also contain a high variety ...201627283704
antitumor activity of annona squamosa seed oil.custard apple (annona squamosa linn.) is an edible tropical fruit, and its seeds have been used to treat "malignant sore" (cancer) and other usage as insecticide. a comparison of extraction processes, chemical composition analysis and antitumor activity of a. squamosa seed oil (aso) were investigated.201627566205
mineral composition, nutritional properties, total phenolics and flavonoids compounds of the atemoya fruit (annona squamosa l. x annona cherimola mill.) and evaluation using multivariate analysis techniques.the atemoya is a hybrid fruit obtained by crossing of cherimoya (annona cherimola mill.) with sweet sop (annona squamosa l.). the information about chemical composition of atemoya is scarce. the mineral composition was evaluated employing inductively coupled plasma optical emission spectrometry (icp oes) and the centesimal composition and the physico-chemical parameters were assessed employing procedures described in the aoac methods. the total phenolic compounds (tpc) and total flavonoids (tf) ...201627598843
ocular toxicity by seeds of annona squamosa (custard apple).custard apple seeds have been used in native medicine from time immemorial for the management of head lice and skin exfoliation. we report six consecutive patients who developed toxic keratoconjunctivitis within 6-12 h of ocular exposure to custard apple seeds. the use of topical steroid worsens the toxicity and predisposes to the development of microbial keratitis in such cases. patients showed a good response to primary treatment with topical fortified antibiotics and lubricants. this case ser ...201627688289
the transition from somatic to germline identity shows conserved and specialized features during angiosperm evolution.how and why specific plant cells adopt germline identity during ovule development has proved challenging to address, and the pathways that are active in the ovules of basal/early-divergent angiosperms possessing a multilayered nucellus are still unclear. here, we compare megasporogenesis between two early-divergent angiosperms (annona cherimola and persea americana) and the evolutionarily derived arabidopsis thaliana, studying the three-dimensional spatial position of the megaspore mother cell ( ...201627878998
antihyperglycemic activity of the leaves from annona cherimola miller and rutin on alloxan-induced diabetic rats.annona cherimola, known as "chirimoya" has been reported in mexican traditional medicine for the treatment of diabetes.201728250646
incidence and growth of salmonella enterica on the peel and pulp of avocado (persea americana) and custard apple (annona squamosa).the aim of this study was to assess the incidence and to estimate the growth kinetic parameters (maximum growth rate, μ; lag time, λ; and maximum population, κ) of salmonella on the peel and pulp of avocado (perseaamericana var. americana) and custard apple (annona squamosa l.) as affected by temperature (10-30°c). the incidence of salmonella was assessed on the peel and pulp of the fruits (n=200 of each fruit), separately, totalizing 800 analyses. only three samples of custard apple pulp were p ...201627393884
[analysis of the microbiological quality and potential presence of listeria monocytogenes in custard apple (annona muricata), mango (mangifera indica) and passion fruit (passiflora edulis) pulps from costa rica].the objective of this work was to determine some of the indicators associated to shelf life, hygiene, process and storage conditions for some of custard apple, mango and passion fruit pulps distributed by the main supermarket chains of the metropolitan area of san josé, costa rica, as well as to examine the potential presence of listeria monocytogenes in them. sixty fruit pulp samples were analyzed. tests included ph determination, total aerobic plate count, yeasts and mold count, lactic bacteri ...201324167958
antiprotozoal constituents from annona cherimola miller, a plant used in mexican traditional medicine for the treatment of diarrhea and dysentery.annona cherimola miller (annonaceae) is a medicinal plant frequently recommended in mexican traditional medicine for the treatment of gastrointestinal disorders such as diarrhea and dysentery.201728216899
two cold-induced family 19 glycosyl hydrolases from cherimoya (annona cherimola) fruit: an antifungal chitinase and a cold-adapted chitinase.two cold-induced chitinases were isolated and purified from the mesocarp cherimoyas (annona cherimola mill.) and they were characterised as acidic endochitinases with a mr of 24.79 and 47.77kda (achi24 and achi48, respectively), both family 19 glycosyl hydrolases. these purified chitinases differed significantly in their biochemical and biophysical properties. while both enzymes had similar optimal acidic ph values, achi24 was enzymatically active and stable at alkaline ph values, as well as dis ...201323890591
efficacy of annona squamosa on wound healing in streptozotocin-induced diabetic rats.annona squamosa l. (annonaceae), commonly known as custard apple, mainly used for its edible fruit, is also recognised with numerous medicinal properties. as there is no report on the efficacy of this plant for wound healing, we examined the efficacy of ethanolic extract of a. squamosa leaves on wound repair in streptozotocin-nicotinamide-induced diabetic rats. open excision wounds were made on the back of rats. the drug at a dosage of 100 mg/kg body wt was reconstituted in 200 µl of phosphate b ...201222233431
Antidepressant-like effects of an alkaloid extract of the aerial parts of Annona cherimolia in mice.Several species of Annona (Annonaceae) are used in traditional Mexican medicine by their anti-anxiety, anticonvulsant and tranquilizing properties. It has been reported that the alkaloids isolated from some species of the Annona have affinity to serotonergic 5-HT(1A) receptors and modulate dopaminergic transmission, which is involved in depressive disorders.201222101086
effects of processing methods on composition and functionality of volatile components isolated from immature fruits of atemoya.atemoya is one of the most important commercial fruits of the family annonaceae. the immature fruits of atemoya amply produced from a fruit-thinning process is normally regarded as waste and discarded. this research aimed at studying antimicrobial, antioxidant, and anti-inflammatory activities of the essential oil (eo) isolated from the immature fruits to explore its potential application. the fruits were subjected to different drying methods: solar drying (sd), oven drying at 30°c (od-30), and ...201626920282
potent cryoprotective activity of cold and co2-regulated cherimoya (annona cherimola) endochitinase.a cryoprotective chitinase (bchi14) was isolated and purified from the mesocarp of co(2)-treated cherimoya fruit (annona cherimola mill.) stored at chilling temperature by anion exchange and chromatofocusing chromatography. this hydrolase was characterized as an endochitinase with a m(r) of 14.31 kda and a pi of 8.26, belonging to the family 19 of glycosyl hydrolases (gh19). while it was stable over a wide ph range and active in a broad acidic ph range, it had an optimum ph of 7.0. its optimum t ...201020576315
an overview of fruit allergy and the causative allergens.plant allergens, being one of the most widespread allergenic substances, are hard to avoid. hence, their identification and characterization are of prime importance for the diagnosis and treatment of food allergy. the reported allergies to fruits mainly evoke oral allergy syndrome caused by the presence of cross-reactive ige to certain pollens and thus, allergy to fruits has also been linked to particular pollens. many fruit allergies are being studied for their causative allergens, and are bein ...201526549334
in vitro susceptibility of entamoeba histolytica and giardia lamblia to plants used in mexican traditional medicine for the treatment of gastrointestinal disorders.in our search for new antiprotozoal chemotherapy, we collected a selection of 26 plants used in mexican traditional medicine for the treatment of gastrointestinal disorders. methanolic extracts of these species were screened for their antiprotozoal activity against entamoeba histolytica and giardia lamblia trophozoites using in vitro tests. among the tested extracts, the derivates of following species showed selectivity and significant antiprotozoal activity: chiranthodendron pentadactylon, anno ...200616846708
differential cytotoxic effects of annona squamosa seed extracts on human tumour cell lines: role of reactive oxygen species and glutathione.annonaceous acetogenins are a new class of compounds that have been reported to have potent pesticidal, parasiticidal, anti-microbial, cell growth inhibitory activities. in this study, organic and aqueous extracts from the defatted seeds of annona squamosa (custard apple) were tested on different human tumour cell lines for antitumoural activity. while organic and aqueous extracts induced apoptosis in mcf-7 and k-562 cells, they failed to do so in colo-205 cells. treatment of mcf-7 and k-562 cel ...200515886460
permanent genetic resources: development of 52 new polymorphic ssr markers from cherimoya (annona cherimola mill.): transferability to related taxa and selection of a reduced set for dna fingerprinting and diversity studies.fifty-two single locus polymorphic microsatellites were developed using two genomic libraries digested with haeiii and rsai of cherimoya cv. fino de jete enriched in ct/ag repeats. a total of 222 alleles were detected with the selected simple sequence repeats (ssrs). the observed and expected heterozygosities ranged from 0.08 to 0.73 and from 0.20 to 0.84, respectively. most of the ssrs were transferable to other species in the annonaceae. a set of 20 microsatellites was selected to facilitate t ...200821585779
a uterotonic substance from annona cherimola seeds.water extract from seeds of annona cherimola was tested in vitro on uterus strips of nongravid guinea-pigs. the extract resulted in possessing utero-constrictive properties due to the presence of a gluco-steroidal substance isolated from it.19807211746
evaluation of cholesterol absorption and biosynthesis by decoctions of annona cherimola leaves.decoctions of the leaves of annona cherimola mill. are traditionally used in azores to treat hypercholesterolemia. although they are sold and consumed by people in order to improve their health, these are products that cannot be sold with claims for health benefits as they have never been studied scientifically.201324095697
characterization and gene cloning of an acidic thaumatin-like protein (tlp 1), an allergen from sapodilla fruit (manilkara zapota).allergy to sapodilla (manilkara zapota) fruit ingestion is rare. an independent study from our group has identified a basic thaumatin-like protein (tlp 2) as the major allergen. the present study was aimed at identifying and characterizing additional allergens from sapodilla.201324060761
solid-phase total synthesis of cherimolacyclopeptide e and discovery of more potent analogues by alanine screening.cherimolacyclopeptide e (1) is a cyclic hexapeptide obtained from annona cherimola, reported to be cytotoxic against the kb (human nasopharyngeal carcinoma) cell line. the solid-phase total syntheses of this cyclic peptide and its analogues were accomplished by employing fmoc/tert-butyl-protected amino acids and the kenner sulfonamide safety-catch linker. the synthetic peptide 1 was found to be weakly cytotoxic against four cell lines (molt-4, jurkat t lymphoma, mda-mb-231, and kb). analogues 3 ...201223148652
six cytotoxic annonaceous acetogenins from annona squamosa seeds.custard apple (annona squamosa l.) is an edible tropical fruit, and its seeds had been used in south china as a folk medicine to treat "malignant sore" (cancer) and as an insecticide. phytochemical investigation of the ethanol fraction of custard apple seeds led to the isolation of six new annonaceous acetogenins: annosquacins a-d (1-4), annosquatin a (5) and annosquatin b (6). their structures were elucidated by spectroscopic analysis. compounds 1-4 are adjacent bistetrahydrofuran annonaceous a ...201222953811
custard apple and latex allergy: a new type of cross-reaction? 201222697017
a cytotoxic cyclic heptapeptide from the seeds of annona cherimola.from a methanol extract of the seeds of annona cherimola, a new cyclic heptapeptide, cherimolacyclopeptide c, has been isolated. the structure was elucidated on the basis of the ms/ms fragmentation using a q-tof mass spectrometer equipped with an esi source, extensive 2d nmr experiments, and chemical degradation. cherimolacyclopeptide c exhibited significant in vitro cytotoxic activity against kb cells, with an ic(50) value of 0.072 microm.200415387664
antitumour activity of annona squamosa seed extracts is through the generation of free radicals and induction of apoptosis.the plant, annona squamosa traditionally known as custard apple possesses potent bioactive principles in all its parts. the effect of aqueous and organic extracts from defatted seeds of a. squamosa was studied on a rat histiocytic tumour cell line, ak-5. both the extracts caused significant apoptotic tumour cell death with enhanced caspase-3 activity, down regulation of antiapoptotic genes bcl-2 and bcl(xl), and enhanced the generation of intracellular ros, which correlated well with the decreas ...200422900348
pollen performance, cell number, and physiological state in the early-divergent angiosperm annona cherimola mill. (annonaceae) are related to environmental conditions during the final stages of pollen development.pollen performance is an important determinant for fertilization success, but high variability in pollen behavior both between and within species occurs in different years and under varying environmental conditions. annona cherimola, an early-divergent angiosperm, is a species that releases a variable ratio of bicellular and tricellular hydrated pollen at anther dehiscence depending on temperature. the presence of both bi- and tricellular types of pollen is an uncommon characteristic in angiospe ...201222569629
mapping genetic diversity of cherimoya (annona cherimola mill.): application of spatial analysis for conservation and use of plant genetic resources.there is a growing call for inventories that evaluate geographic patterns in diversity of plant genetic resources maintained on farm and in species' natural populations in order to enhance their use and conservation. such evaluations are relevant for useful tropical and subtropical tree species, as many of these species are still undomesticated, or in incipient stages of domestication and local populations can offer yet-unknown traits of high value to further domestication. for many outcrossing ...201222253801
high co2 atmosphere modulating the phenolic response associated with cell adhesion and hardening of annona cherimola fruit stored at chilling temperature.phenylalanine ammonia-lyase (pal, ec 4.3.1.5.) activity, tanning ability, and polyphenols levels were measured in cherimoya (annona cherimola mill.) fruit treated with 20% co(2) + 20% o(2) + 60% n(2) for 1, 3, or 6 days during chilling temperature (6 degrees c) storage. the residual effect of co(2) after transfer to air was also studied. these observations were correlated with texture and cellular characteristics, visualized by cryo-sem. tanning ability and the early increase in tannin polypheno ...200212475271
identification and characterization of genes differentially expressed in cherimoya (annona cherimola mill) after exposure to chilling injury conditions.cherimoyas (annona cherimola), like other subtropical/tropical fruits, are susceptible to damage from exposure to temperatures between 0 and 5 °c (chilling injury, ci), which may affect fruit quality. to increase our understanding of the molecular mechanisms involved in the ci response, a forward suppression subtractive hybridization (ssh) cdna library was constructed. in this work, we obtained 75 genes that could potentially be involved in the ci response. the ci induced activation of genes tha ...201122087802
floral development and floral phyllotaxis in anaxagorea (annonaceae).background and aims anaxagorea is the phylogenetically basalmost genus in the large tropical annonaceae (custard apple family) of magnoliales, but its floral structure is unknown in many respects. the aim of this study is to analyse evolutionarily interesting floral features in comparison with other genera of the annonaceae and the sister family eupomatiaceae. methods live flowers of anaxagorea crassipetala were examined in the field with vital staining, liquid-fixed material was studied with sc ...201121821626
four alkaloids from annona cherimola.four alkaloids, annocherine a, annocherine b, cherianoine, and romucosine h, along with one known alkaloid, artabonatine b, were isolated from the meoh extract of the stems of annona cherimola. their structures were identified on the basis of both analysis of their spectral data and from chemical evidence.200111314964
stigmatic receptivity in a dichogamous early-divergent angiosperm species, annona cherimola (annonaceae): influence of temperature and humidity.a variety of mechanisms to prevent inbreeding have arisen in different angiosperm taxa during plant evolution. in early-divergent angiosperms, a widespread system is dichogamy, in which female and male structures do not mature simultaneously, thus encouraging cross pollination. while this system is common in early-divergent angiosperms, it is less widespread in more recently evolved clades. an evaluation of the consequences of this system on outbreeding may provide clues on this change, but this ...201121613115
grapefruit as a host for the west indian fruit fly (diptera: tephritidae).the most common hosts for the west indian fruit fly, anastrepha obliqua (macquart) (diptera: tephritidae) are fruit in the family anacardiaceae (mango [mangifera l.] and mombin [spondias l.] species). however, similar to many of the tropical fruit flies of major economic importance, this species attacks several other families of crop fruit, including annonaceae (cherimoya, annona cherimola mill.), myrtaceae (guava, psidium l.), oxalidaceae (carambola, averrhoa carambola l.), passifloraceae (gran ...201121404839
ige-mediated hypersensitivity to custard-apple. 19979201378
segregation distortion of isozyme loci in cherimoya (annona cherimola mill).the allelic segregation of 13 isozyme loci in hand-fertilized heterozygous cherimoya trees (annona cherimola mill.) has been studied. we analyzed 63 locus x progeny combinations and found non-mendelian segregation in 12 cases. the sequential bonferroni method revealed only eight cases of non-mendelian segregation; these have been investigated with several chi-square tests to discover what processes were involved. gametic selection appears to be the main contributor, although zygotic selection se ...199624162303
interpretation of triose phosphate isomerase isozymes in the cherimoya (annona cherimola mill.).detailed interpretation of triose phosphate isomerase (tpi) isozymes in seed plants has been restricted to only a few species. three sets of tpi bands are regularly observed in the cherimoya (annona cherimola), a primitive angiosperm. the slowest, set i, is expressed as one or three bands; the second-slowest set ii, as one or two bands; and the fastest, set iv, as one or three bands. a faint set iii, just cathodal to set iv, is detected rarely with overstaining. set iv bands are expressed in mac ...19883377755
pharmacological screening of annona cherimola for antihyperlipidemic potential.high blood cholesterol levels are consistently associated with higher risk of coronary heart disease, and other life-threatening cardiovascular and cerebro-vascular damage, including fatal strokes. in the present study methanolic extract of annona cherimola was evaluated for its antihyperlipidemic potential. acute hyperlipidemia was induced by single administration of triton wr 1339 (tyloxapol) intra-peritoneally. there was a significant dose dependent decrease in plasma total cholesterol, trigl ...201124826004
four new ent-kaurane diterpenoids from the fruits of annona cherimola.four new ent-kaurane diterpenoids (16r)-ent-kauran-17,19-diol (1), (16r)-17-hydroxy-ent-kauran-19-oic acid (2), (16s)-17-hydroxy-ent-kauran-19-oic acid (3), and (16r)-17-dimethoxy-ent-kauran-19-oic acid (4) have been isolated from the fresh fruits of annona cherimola together with eight known compounds. their structures are determined on the basis of spectroscopic data and optical rotation.201020460813
the progamic phase of an early-divergent angiosperm, annona cherimola (annonaceae).recent studies of reproductive biology in ancient angiosperm lineages are beginning to shed light on the early evolution of flowering plants, but comparative studies are restricted by fragmented and meagre species representation in these angiosperm clades. in the present study, the progamic phase, from pollination to fertilization, is characterized in annona cherimola, which is a member of the annonaceae, the largest extant family among early-divergent angiosperms. beside interest due to its phy ...201019939980
pollen development in annona cherimola mill. (annonaceae). implications for the evolution of aggregated pollen.in most flowering plants, pollen is dispersed as monads. however, aggregated pollen shedding in groups of four or more pollen grains has arisen independently several times during angiosperm evolution. the reasons behind this phenomenon are largely unknown. in this study, we followed pollen development in annona cherimola, a basal angiosperm species that releases pollen in groups of four, to investigate how pollen ontogeny may explain the rise and establishment of this character. we followed poll ...200919874617
the coexistence of bicellular and tricellular pollen in annona cherimola (annonaceae): implications for pollen evolution.most angiosperms release bicellular pollen. however, in about one-third of extant angiosperms, the second pollen mitosis occurs before anthesis such that pollen is tricellular upon release. the shift from bicellular to tricellular development has occurred several times independently, but its causes are largely unknown. in this work, we observed the coexistence of both kinds of pollen at anther dehiscence in annona cherimola, a species that belongs to the basal angiosperm family annonaceae. exami ...200921628235
comparative analysis of the volatile fraction from annona cherimola mill. cultivars by solid-phase microextraction and gas chromatography-quadrupole mass spectrometry detection.the analysis of volatile compounds in funchal, madeira, mateus and perry vidal cultivars of annona cherimola mill. (cherimoya) was carried out by headspace solid-phase microextraction (hs-spme) combined with gas chromatography-quadrupole mass spectrometry detection (gc-qmsd). hs-spme technique was optimized in terms of fibre selection, extraction time, extraction temperature and sample amount to reach the best extraction efficiency. the best result was obtained with 2g of sample, using a divinyl ...200919064096
nutritional and phytochemical composition of annona cherimola mill. fruits and by-products: potential health benefits.annona cherimola mill., commonly known as cherimoya, is a tropical fruit well known due to its tasty flavour. in the present study the antioxidant activity of pulp, peel and seeds of four cultivars from a. cherimola mill. from madeira island (madeira, funchal, perry vidal and mateus ii) was analysed. moreover, nutritional composition (proximates and vitamins) and bioactive compounds content were determined. the peel of madeira cultivar showed the highest antioxidant capacity, with an ec50 of 0.9 ...201626433307
dna barcoding of perennial fruit tree species of agronomic interest in the genus annona (annonaceae).the dna barcode initiative aims to establish a universal protocol using short genetic sequences to discriminate among animal and plant species. although many markers have been proposed to become the barcode of plants, the consortium for the barcode of life (cbol) plant working group recommended using as a core the combination of two portions of plastid coding region, rbcl and matk. in this paper, specific markers based on matk sequences were developed for 7 closely related annona species of agro ...201526284104
storage behavior of mango as affected by post harvest application of plant extracts and storage conditions.the use of plant extracts could be a useful alternative to synthetic fungicides in the post harvest handling of fruits and vegetables. the aim of this study was to access the efficacy of extracts obtained from four plants (neem, pongamia, custard apple leaf and marigold flowers) on the extension of shelf life of mango fruits cv. dashehri under two storage conditions (cool store and ambient condition). the fruits were treated with 2 concentrations of each plant extracts (10 % and 20 %) were place ...201425328189
arabinogalactan proteins, pollen tube growth, and the reversible effects of yariv phenylglycoside.arabinogalactan proteins (agps) are abundant complex macromolecules involved in both reproductive and vegetative plant growth. they are secreted at pollen tube tips in lilium longiflorum. here, we report the effect of the (beta-d-glucosyl)3 yariv phenylglycoside, known to interact with agps, on pollen tube extension in several plant species. in annona cherimola the yariv reagent clearly inhibited pollen tube extension within 1-2 h of treatment, as demonstrated previously for l. longiflorum, but ...200211926071
determination of some physicochemical characteristics, bioactive compounds and antioxidant activity of tropical fruits from yucatan, mexico.the aim to the study was to determine the physicochemical composition, bioactive compounds and antioxidant activity of fruits from yucatan, mexico such as star apple, cashew, mombin, mamey sapote, white sapote, sugar apple, sapodilla, dragon fruit, nance, ilama, custard apple, mamoncillo and black sapote. the physicochemical characteristics were different between fruits and were good sources of bioactive compounds. the edible part with the highest values of antioxidant activity were mamoncillo, ...201424444968
influence of gamma irradiation and benzyl adenine on keeping quality of custard apple fruits during storage.the custard apple (annona squamosa) fruits were procured from local market, irradiated with radiation doses 0, 0.25, 0.50, 0.75, 1.00, 1.25, 1.50, 1.75 kgy and then treated with benzyl adenine (50 and 100 part per million) and stored at ambient temperature (25 ± 5 °c, relative humidity 90 ± 2%) for 12 days. the treated fruits were evaluated for sensory (viz; flavour, texture, internal and external colour) and chemical constituents (viz; total soluble solids, titrable acidity, ascorbic acid, free ...201324426000
ascorbic acid contents of pakistani fruits and vegetables.fresh fruits and vegetables are good sources of vitamin c which is known for its antioxidant and immune-enhancing effects. the objective of this study was to determine ascorbic acid (vitamin c) contents of regularly consumed fruits and vegetables available in pakistani markets. most commonly used fresh fruits and vegetables were homogenized in 5% trichloroacetic acid, and ascorbic acid contents in the extracts were determined using a spectrophotometric method. banana, custard apple, orange, lemo ...200617105704
latex-vegetable syndrome due to custard apple and aubergine: new variations of the hevein symphony.an increasing number of vegetables with crossreactions to latex are being described in patients with latex-vegetable syndrome. we present two of these vegetables, custard apple linked in two previous cases with latex sensitisation, and aubergine, that had not been described up to now in patients with latex sensitisation. the diagnosis of both cases was based on the clinical history, positive skin prick test (spt) and specific ige to the offending vegetables, as well as to positive spt and specif ...200516433216
rain forest provides pollinating beetles for atemoya crops.small beetles, usually species of nitidulidae, are the natural pollinators of atemoya (annona squamosa l. x a. cherimola mill. hybrids; custard apple) flowers but commercial atemoya growers often need to carry out labor-intensive hand pollination to produce enough high-quality fruit. because australian rain forest has plant species in the same family as atemoya (annonaceae) and because many rain forest plants are beetle pollinated, we set out to discover whether tropical rain forest in far north ...200516156571
cherimolacyclopeptide d, a novel cycloheptapeptide from the seeds of annona cherimola.in a chemical investigation of the seeds of annona cherimola, a natural cyclic heptapeptide, cherimolacyclopeptide d, were isolated and purified by hplc with three known cyclic peptides, cherimolacyclopeptides a, b and c. the structure was established by various analyses including ms/ms fragmentation, spectroscopic and chemical evidences.200515771892
malate metabolism and adaptation to chilling temperature storage by pretreatment with high co2 levels in annona cherimola fruit.in this study we focused on the effect of a pretreatment with high (20%) co2 levels on malic acid metabolism in cherimoya (annona cherimola mill) fruit stored at chilling temperature. we analyzed the activity of phosphoenolpyruvate carboxylase (pepc), malate dehydrogenase (mdh), and the nadp-malic enzyme (nadp-me), involved in the carboxylation/decarboxylation of malate. our results show that co2 treatment, which improves tolerance to prolonged storage at chilling temperature, was closely linked ...200415264911
oral allergic syndrome after ingestion of custard apple. 200312653803
phosphoenolpyruvate carboxylase from cherimoya fruit: properties, kinetics and effects of high co(2).phosphoenolpyruvate carboxylase (pepc, ec 4.1.1.31) regulatory properties were studied in non-photosynthetic (mesocarp) and photosynthetic (peel) tissues from cherimoya (annona cherimola mill.) fruit stored in air, in order to gain a better understanding of in vivo enzyme regulation. analyses were also performed with fruit treated with 20% co(2)-20% o(2) to define the role of pepc as part of an adaptive mechanism to high external carbon dioxide levels. the results revealed that the special kinet ...200111730863
anti-headlice activity of a preparation of annona squamosa seed extract.various parts of annona squamosa linn. (custard apple) have long been used in thai folk medicine. the effectiveness of organic solvent extracts of custard apple seeds and leaves against headlice has been reported. the present study is focused on the efficacy and stability of seed extract prepared as a cream. a petroleum ether extract of seeds was prepared as a 20% w/w oil in water cream and tested for anti-headlice activity in school girls. after a 3 hour-hair exposure to 20 g of freshly prepare ...200011414452
high-performance liquid chromatographic screening of chlorophyll derivatives produced during fruit storage.reversed-phase high-performance liquid chromatography with photodiode array and fluorescence detection was applied to the systematic screening of chlorophylls and derivative pigments. the chromatographic procedure proposed made it possible to successfully separate and identify eight chlorophyll derivatives (the a and b forms of chlorophyll, chlorophyllide, pheophytin and pheophorbide) by using a linear gradient of methanol, acetone and ammonium acetate. the method has been routinely applied to s ...200010722106
aromin-a, an annonaceous acetogenin from annona cherimola.chromatographic fractionation of a meoh extract from the stems of annona cherimola led to the isolation of a new non-adjacent, bis-thf ring acetogenin (one thf ring being at c-4 and the other at c-16), aromin-a (1) along with the known cytotoxic acetogenin squamocin (2). the structures of these isolates were established by means of mass spectrometry and spectroscopic techniques.199910382318
haploid plants from in vitro anther culture of annona squamosa linn.haploid plants were induced from anther callus of annona squamosa linn. (custard apple) on a nitsch basal medium supplemented with 6-benzyl-aminopurine and naphthalene acetic acid. when naphthalene acetic acid was replaced by indole-3-acetic acid only multiple shoots were obtained. pretreatment (chilling, centrifugation, reduced atmospheric pressure etc.) of the flowers was not effective but dissection of the flowers in a suspension of activated charcoal and sucrose was found essential. the anth ...198324258051
browning in annona cherimola fruit: role of polyphenol oxidase and characterization of a coding sequence of the enzyme.cherimoya (annona cherimola mill.) fruit is an attractive candidate for food processing applications as fresh cut. however, along with its desirable delicate taste, cherimoya shows a marked susceptibility to browning. this condition is mainly attributed to polyphenol oxidase activity (ppo). a general lack of knowledge regarding ppo and its role in the oxidative loss of quality in processed cherimoya fruit requires a better understanding of the mechanisms involved. the work carried out included t ...200717907770
application of low-pressure gas chromatography/tandem mass spectrometry to the determination of pesticide residues in tropical fruits.a multiresidue method has been developed for determining pesticide residues in the tropical fruits kiwi, custard apple, and mango. the intended purpose of the method is for regulatory analyses of commodities for pesticides that have established maximum residue limits. a fast and simple extraction method with cyclohexane-ethyl acetate (1 + 1, v/v) and a high-speed homogenizer was optimized. pressurized liquid extraction was evaluated as an alternative automated extraction technique. the pesticide ...201217760354
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