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[population dynamics of rhipicephalus pusillus gil collado, 1938 (acarina, ixodidae) in 1986-1987 in south-eastern spain: modelling of the biological cycle].the evolution of the rhipicephalus pusillus population, a parasite of the european wild rabbit, oryctolagus cuniculus, is studied in a locality of the province of granada (spain), in 1986-1987. the moving average technique was applied to the sampling data (358 hosts), for each stage of the postembryonic cycle. this technique permitted us, together with the habitual usage of the statistics, to understand in a more complete manner, the evolution of daily changes which affect the average number of ...19892596808
rickettsiae of the spotted fever group associated with the host-parasite system oryctolagus cuniculi/rhipicephalus pusillus.wild rabbits--oryctolagus cuniculi--living in large numbers in a protected zone of tuscany, the park of migliarino--san rossore--massaciuccoli, showed to be carriers of the hard tick rhipicephalus pusillus, previously observed in north africa and sicily. antibodies to rickettsia conorii and r. slovaca were detected in 78.9 per cent of the wild rabbits captured in that area. seroconversion towards r. conorii was also observed in guinea pigs inoculated with homogenates of r. pusillus parasitizing ...19883195257
18s rrna gene sequences and phylogenetic relationships of european hard-tick species (acari: ixodidae).the complete 18s rrna gene sequences of the following six european hard-tick species were obtained by direct pcr cycle sequencing and silver-staining methods: rhipicephalus pusillus, boophilus annulatus, dermacentor marginatus, hyalomma lusitanicum, haemaphysalis punctata, and ixodes ricinus. differences observed in the sequence alignment of these six species together with the 18s rrna gene sequences of 13 other hard-tick species demonstrate that this gene is a good marker for supraspecific diff ...19989491423
general review of tick species present in portugal.at present, 24 species are known to occur in portugal: argas vespertilionis, ornithodoros maritimus and ornithodoros erraticus in argasidae; ixodes acuminatus, ixodes bivari, ixodes canisuga, ixodes frontalis, ixodes hexagonus, ixodes ricinus, ixodes simplex simplex, ixodes ventalloi, ixodes vespertilionis, dermacentor marginatus, dermacentor pictus', haemaphysalis hispanica, haemaphysalis inermis, haemaphysalis punctata, rhipicephalus bursa, rhipicephalus pusillus, rhipicephalus sanguineus, rhi ...199911071535
rickettsia sibirica isolation from a patient and detection in ticks, portugal.we report the first isolation of rickettsia sibirica (strain mongolotimonae) from the blood of a patient and detection by polymerase chain reaction (pcr) of the rickettsia in a rhipicephalus pusillus tick collected from a dead mongoose (herpestes ichneumon) in the alentejo region, portugal. we describe also the first pcr detection of a new rickettsia strain that is related to r. sibirica.200616836827
ectoparasites of the endangered iberian lynx lynx pardinus and sympatric wild and domestic carnivores in spain.ectoparasites can cause important skin disorders in animals and can also transmit pathogens. the iberian lynx lynx pardinus has been stated to be the most endangered felid in the world and such vector-borne pathogens may threaten its survival. we surveyed 98 wild carnivores (26 iberian lynxes, 34 red foxes vulpes vulpes, 24 egyptian mongooses herpestes ichneumon, 11 common genets genetta genetta, two eurasian badgers meles meles, one polecat mustela putorius) and 75 domestic but free-ranging car ...200717897365
lymphangitis in a portuguese patient infected with rickettsia sibirica. 200818325289
pathogens and symbionts in ticks: a survey on tick species distribution and presence of tick-transmitted micro-organisms in sardinia, italy.a total of 1485 adult ticks were collected from mammalian hosts in south-eastern sardinia, italy, during the years 2007-2008. ticks were identified and tested by pcr analysis for presence of rickettsia species of the spotted fever group, ehrlichia canis, anaplasma phagocytophilum, coxiella burnetii, bartonella species and leishmania species. among all tick species examined (rhipicephalus sanguineus, rhipicephalus turanicus, rhipicephalus bursa, rhipicephalus pusillus, hyalomma marginatum margina ...201020884769
the hard-tick fauna of mainland portugal (acari: ixodidae): an update on geographical distribution and known associations with hosts and pathogens.this work is an updated revision of the available information on portuguese ixodid tick species. it includes data on tick biology, ecology, taxonomy and host/pathogen-associations. the current list of portuguese ixodid ticks comprises twenty species: dermacentor marginatus (sulzer, 1776), dermacentor reticulatus (fabricius, 1794), haemaphysalis hispanica gil collado, 1938, haemaphysalis inermis birula, 1895, haemaphysalis punctata canestrini & fanzago, 1878, hyalomma lusitanicum koch, 1844, hyal ...201121452063
presence of bartonella species in wild carnivores of northern spain.the genus bartonella was detected by pcr in 5.7% (12/212) of wild carnivores from northern spain. based on hybridization and sequence analyses, bartonella henselae was identified in a wildcat (felis silvestris), bartonella rochalimae in a red fox (vulpes vulpes) and in a wolf (canis lupus), and bartonella sp. in badgers (meles meles).201122138983
ticks infesting humans in italy and associated pathogens.ticks may transmit a large variety of pathogens, which cause illnesses in animals and humans, commonly referred to as to tick-borne diseases (tbds). the incidence of human tbds in italy is underestimated because of poor surveillance and the scant amount of studies available.201425023709
tick-borne infections in human and animal population worldwide.the abundance and activity of ectoparasites and its hosts are affected by various abiotic factors, such as climate and other organisms (predators, pathogens and competitors) presenting thus multiples forms of association (obligate to facultative, permanent to intermittent and superficial to subcutaneous) developed during long co-evolving processes. ticks are ectoparasites widespread globally and its eco epidemiology are closely related to the environmental conditions. they are obligatory hematop ...201527047089
the recent evolution of a maternally-inherited endosymbiont of ticks led to the emergence of the q fever pathogen, coxiella burnetii.q fever is a highly infectious disease with a worldwide distribution. its causative agent, the intracellular bacterium coxiella burnetii, infects a variety of vertebrate species, including humans. its evolutionary origin remains almost entirely unknown and uncertainty persists regarding the identity and lifestyle of its ancestors. a few tick species were recently found to harbor maternally-inherited coxiella-like organisms engaged in symbiotic interactions, but their relationships to the q fever ...201525978383
update on tick-borne rickettsioses around the world: a geographic approach.tick-borne rickettsioses are caused by obligate intracellular bacteria belonging to the spotted fever group of the genus rickettsia. these zoonoses are among the oldest known vector-borne diseases. however, in the past 25 years, the scope and importance of the recognized tick-associated rickettsial pathogens have increased dramatically, making this complex of diseases an ideal paradigm for the understanding of emerging and reemerging infections. several species of tick-borne rickettsiae that wer ...024092850
morphological and genetic diversity of rhipicephalus sanguineus sensu lato from the new and old worlds.the taxonomic status of the brown dog tick (rhipicephalus sanguineus sensu stricto), which has long been regarded as the most widespread tick worldwide and a vector of many pathogens to dogs and humans, is currently under dispute.201323880226
clustered cases of rickettsia sibirica mongolitimonae infection, france. 023460995
human infection with rickettsia sibirica mongolitimonae, spain, 2007-2011.human infection with rickettsia sibirica mongolitimonae was initially reported in 1996, and reports of a total of 18 cases have been published. we describe 6 additional cases that occurred in the mediterranean coast region of spain during 2007-2011. clinicians should consider this infection in patients who have traveled to this area.023343524
update on epidemiology of canine babesiosis in southern france.canine babesiosis is an emerging or re-emerging disease caused by babesia and theileria protozoans, also called piroplasms, transmitted by ixodid ticks. in europe, four etiological agents have been identified to date, namely babesia canis, b. vogeli, b. gibsoni and theileria annae. france has a high prevalence of canine babesiosis and two tick species, dermacentor reticulatus and rhipicephalus sanguineus, are supposed to transmit b. canis and b. vogeli respectively. in southern france, where dog ...201526303260
the role of cats in the eco-epidemiology of spotted fever group diseases.mediterranean spotted fever (msf), whose etiological agent is r. conorii, is one of the oldest described vector-borne infectious diseases. although it is endemic in the mediterranean area, clinical cases have also been reported in other regions. r. massiliae-bar29 is related to msf cases. this strain is distributed worldwide. r. conorii and r. massiliae-bar29 are transmitted by ticks. dogs are considered the sentinel of r. conorii infection. cats could also be involved in their transmission. ric ...201425084969
detection of vector-borne pathogens in cats and their ectoparasites in southern italy.vector-borne pathogens are the subject of several investigations due to the zoonotic concern of some of them. however, limited data are available about the simultaneous presence of these pathogens in cats and their ectoparasites. the aim of the present study was to define the species of ectoparasites found on cats as well as to investigate vector-borne pathogens in cats and their ectoparasites in southern italy.201627160725
ticks and associated pathogens collected from cats in sicily and calabria (italy).limited information is available about the species of ticks infesting the cat and the pathogens that they harbor. the aims of the present study were to identify the species of ticks removed from cats living in sicily and calabria (italy) and to detect dna of vector-borne pathogens in the same ticks.201526445916
coinfection with "rickettsia sibirica subsp. mongolotimonae" and rickettsia conorii in a human patient: a challenge for molecular diagnosis tools.rickettsioses are zoonoses transmitted by vectors. more than one agent can coexist in vectors. although vectors may transmit more than one microorganism to humans, information on dual infections is scarce. we present a case of a patient with an atypical rickettsiosis diagnosis in whom two species of rickettsia were detected.201526135877
rickettsia massiliae in the canary islands. 022531111
detection of rickettsia sibirica mongolitimonae by using cutaneous swab samples and quantitative pcr. 024655579
feline and canine leishmaniosis and other vector-borne diseases in the aeolian islands: pathogen and vector circulation in a confined environment.vector-borne diseases (vbds) are prevalently investigated in dogs. studies on feline vbds are scant, though feline leishmaniosis (fel) is increasingly recognised as a disease of cats in endemic areas. comprehensive investigations on the distribution of vbds in populations of cats and dogs living in relatively small geographical areas, such as islands, are currently lacking. in this study the prevalence of leishmania infantum and other vbd pathogens was assessed in cohorts of cats and dogs living ...201728288759
[lymphangitis-associated rickettsiosis caused by rickettsia sibirica mongolitimonae].rickettsia sibirica mongolitimonae was first isolated 20 years ago in asia but has now been identified on three continents. hyalomma spp. and rhipicephalus pusillus ticks are vectors but only a small number of cases have been reported to date, mainly on the mediterranean coast. this bacterium induces the lymphangitis-associated rickettsiosis, a still unfamiliar rickettsiosis that is mainly characterized by fever with a rope-like lymphangitis and/or lymphadenopathy and skin eschar occurring after ...201424034636
long term study of ixodid ticks feeding on red deer (cervus elaphus) in a meso-mediterranean climate.red deer (cervus elaphus l.) are very valuable in trophy-hunting but also contribute to the preservation of natural areas. they are affected by many parasites and pathogens, including hard ticks that are not only important parasites themselves but can also act as vectors and/or reservoirs of pathogens. tick phenology is complex insofar as population dynamics depend on environmental conditions, vegetation, host availability and their own intrinsic characteristic. ticks were collected monthly from ...201626715543
seasonal dynamics of ixodid ticks on wild rabbits oryctolagus cuniculus (leporidae) from central spain.rabbit hemorrhagic disease and myxomatosis caused a decline in the rabbit population in the second half of the last century. despite some recovery, the risk of vector-borne disease is present and thus the importance of controlling vector populations. in the current study, we describe the ixodid tick fauna in wild rabbit in a natural reserve in ciudad real (central spain) during the course of two 3-year periods (2007-2009 and 2012-2014). of all the ticks collected on average 72.5 % were larvae, 2 ...201627411939
long-term ecological study of host-seeking adults of hyalomma lusitanicum (acari: ixodidae) in a meso-mediterranean climate.from january 2007 to december 2014, three representative meso-mediterranean bioclimatic environment types were sampled monthly using blanket-dragging techniques to determine the tick abundance rate. hyalomma lusitanicum koch, 1844 was the most prevalent species (96.58%) followed by dermacentor marginatus sulzer, 1776; rhipicephalus pusillus gil collado, 1936; and rhipicephalus bursa canestrini and fanzago, 1878. h. lusitanicum adults begin questing activity around march, numbers rising quickly r ...201626477051
one particular anaplasma phagocytophilum ecotype infects cattle in the camargue, france.anaplasma phagocytophilum is a zoonotic tick-borne pathogen responsible for granulocytic anaplasmosis, a mild to a severe febrile disease that affects man and several animal species, including cows and horses. in europe, i. ricinus is the only proven vector for this pathogen, but studies suggest that other tick genera and species could be involved in its transmission. our objective was to assess the presence and genetic diversity of a. phagocytophilum in domestic animals and different tick speci ...201728764743
detection of coxiella burnetii dna in peridomestic and wild animals and ticks in an endemic region (canary islands, spain).coxiella burnetii, the etiological agent of human q fever, can infect mammals, birds, and arthropods. the canary islands (spain) are considered an endemic territory, with a high prevalence in both humans and livestock. nonetheless, there is no epidemiological information about the wild and peridomestic cycles of c. burnetii. tissue samples from rodents on farms (100) and wild rabbits (129) were collected and assessed by pcr to detect c. burnetii dna. in parallel, ticks were also collected from v ...201728759337
season-long control of flea and tick infestations in a population of cats in the aeolian archipelago using a collar containing 10% imidacloprid and 4.5% flumethrin.cats that have outdoor access are highly exposed to ticks, fleas, mites and flying insects, though the risk to become infested by arthropods is less perceived in cats than in dogs. this has resulted in fewer treatment and prevention options being available for cats than for dogs. a collar containing a combination of 10% imidacloprid and 4.5% flumethrin (seresto®, bayer animal health) is available for cats and licensed with claims against ticks and fleas for 7-8 months. following the assessment o ...201729173546
the effect of excluding ungulates on the abundance of ixodid ticks on wild rabbit (oryctolagus cuniculus).tick abundance depends heavily on host density, so reducing access to the host should reduce tick populations in a determined area. in this study we compared the parasitic index (pi = average number of ticks per rabbit) of two wild rabbit populations separated 16 years ago by a fence keeping ungulates from moving freely. two areas were selected (closed and open) wherein 50 wild rabbits per area were sampled for ticks. the pi in the open area (pi = 989.62) was significantly higher than in the clo ...201728840404
genetic characterization of rhipicephalus sanguineus (sensu lato) ticks from dogs in portugal.the taxonomic status of the brown dog tick rhipicephalus sanguineus (sensu stricto) is a subject of on-going debate; there is a consensus that populations of this tick species should be referred to as r. sanguineus (sensu lato) until its taxonomic status is resolved. recent genetic studies revealed the existence of more than one lineage of r. sanguineus (s.l.) in temperate countries. in this study, we assessed the genetic identity of ticks collected from rural dogs living in several areas locate ...201728285602
genetic analysis of rhipicephalus sanguineus sensu lato ticks parasites of dogs in africa north of the sahara based on mitochondrial dna sequences.the aim of this work was to determine the evolutionary relationship among tick populations of rhipicephalus sanguineus sensu lato distributed in africa north of the sahara and different lineages of r. sanguineus s.l. distributed in different regions of sub-saharan africa, america and europe through the analysis of dna sequences of two mitochondrial genes. one hundred and thirty six 16s rrna gene sequences and twenty-seven 12s rrna gene sequences of r. sanguineus s.l. were analyzed. phylogenetic ...201728495189
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