| effect of carbon sources on germibation of chlamydospores of ustilago scitaminea syd. | | 1971 | 5551953 |
| a maize-specifically expressed gene cluster in ustilago maydis. | the corn pathogen ustilago maydis requires its host plant maize for development and completion of its sexual cycle. we have identified the fungal mig2-1 gene as being specifically expressed during this biotrophic stage. intriguingly, mig2-1 is part of a gene cluster comprising five highly homologous and similarly regulated genes designated mig2-1 to mig2-5. deletion analysis of the mig2-1 promoter provides evidence for negative and positive regulation. the predicted polypeptides of all five gene ... | 2002 | 11849538 |
| [structural changes in sugar cane buds during infection by the fungus ustilago scitaminea]. | the structural changes that take place in sugar cane buds during the infection with ustilago scitaminea were studied. buds of the cultivars sp79-2312 and na56-79, resistant and susceptible to this fungus, were inoculated with teliospores of u. scitaminea. they were placed in trays and maintained in darkness at 31 degrees and 80% relative humidity. thirty buds of each cultivar left without inoculating as controls. the samplings were carried out at 16, 40 and 72 h after inoculation, taking 10 inoc ... | 2002 | 12825980 |
| induced variation in the germination of chlamydospores of ustilago scitaminea. | | 1951 | 14849173 |
| glycoproteins from sugarcane plants regulate cell polarity of ustilago scitaminea teliospores. | saccharum officinarum, cv. mayarí, is a variety of sugarcane resistant to smut disease caused by ustilago scitaminea. sugarcane naturally produces glycoproteins that accumulate in the parenchymatous cells of stalks. these glycoproteins contain a heterofructan as polysaccharide moiety. the concentration of these glycoproteins clearly increases after inoculation of sugarcane plants with smut teliospores, although major symptoms of disease are not observed. these glycoproteins induce homotypic adhe ... | 2005 | 15832677 |
| molecular profiling demonstrates limited diversity amongst geographically separate strains of ustilago scitaminea. | intraspecies diversity within ustilago scitaminea isolates from south africa, reunion island, hawaii and guadeloupe was assessed by rapds, be mating-type gene detection, rdna sequence analysis, microscopy and germination and morphological studies. except for sequence data, the other analyses yielded no differences in the isolates that could be used in a phylogenetic separation. mycelial dna of the sa isolate shared 100% sequence identity with that of mycelial dna cultured from in vitro produced ... | 2005 | 15927742 |
| identification of sugarcane genes induced in disease-resistant somaclones upon inoculation with ustilago scitaminea or bipolaris sacchari. | to understand the molecular basis of a specific plant-pathogen interaction, it is important to identify plant genes that respond to the pathogen attack. amplified fragment length polymorphism (aflp) analysis of cdna was used to identify sugarcane genes differentially expressed in disease-resistant but not in susceptible sugarcane somaclones in response to inoculation with either ustilago scitaminea or bipolaris sacchari (also known as helminthosporium sacchari or drechslera sacchari), causal age ... | 2005 | 16386426 |
| evidence for the dispersal of a unique lineage from asia to america and africa in the sugarcane fungal pathogen ustilago scitaminea. | the basidiomycete ustilago scitaminea sydow, which causes sugarcane smut disease, has been spreading throughout africa and america since the 1940s. the genetic diversity and structure of different populations of this fungus worldwide was investigated using microsatellites. a total of 142 single-teliospore were isolated from 77 distinct whips (sori) collected in 15 countries worldwide. mycelium culture derived from on generation of selfing of these single teliospores were analysed for their polym ... | 2007 | 16979360 |
| associations between dna markers and resistance to diseases in sugarcane and effects of population substructure. | association between markers and sugarcane diseases were investigated in a collection of 154 sugarcane clones, consisting of important ancestors or parents, and cultivars. 1,068 polymorphic aflp and 141 srr markers were scored across all clones. data on the four most important diseases in the australian sugarcane industry were obtained; these diseases being pachymetra root rot (pachymetra chaunorhiza b.j. croft & m.w. dick), leaf scald (xanthomonas albilineans dowson), fiji leaf gall (fiji diseas ... | 2006 | 17047910 |
| differential expression analysis by cdna-aflp of saccharum spp. after inoculation with the host pathogen sporisorium scitamineum. | a differential expression study on the saccharum spp.-sporisorium scitamineum pathogenic interaction was carried out in the susceptible ja60-5 and the resistant m31/45 genotypes. using cdna-aflp analysis, a total of 64 transcript-derived fragments (tdfs) was found to be differentially expressed, with the majority (67.2%) of the differential tdfs up-regulated in the resistant m31/45 cultivar. the plant response against s. scitamineum infection was complex, representing major genes involved in oxi ... | 2008 | 18379790 |
| production of glycolipid biosurfactants, mannosylerythritol lipids, by a smut fungus, ustilago scitaminea nbrc 32730. | a smut fungus ustilago scitaminea nbrc 32730 on sugar cane (saccharum) was found to accumulate a large amount of glycolipids in the culture medium. as a result of structural characterization, the main glycolipid was identified as mel-b, 4-o-beta-(2',3'-di-o-alka(e)noyl-6'-o-acetyl-d-mannopyranosyl)-erythritol. the mel-b was sufficiently produced from a variety of sugars such as sucrose, glucose, fructose, and mannose. olive oil and methyl oleate were also available as carbon sources to produce m ... | 2009 | 19270362 |
| differential gene expression in sugarcane in response to challenge by fungal pathogen ustilago scitaminea revealed by cdna-aflp. | differential gene expression in sugarcane during sugarcane-ustilago scitaminea interaction was conducted in a smut-resistant genotype. using cdna-aflp along with silver staining, a total of 136 transcript-derived fragments (tdfs) were found to be differentially expressed in response to challenge by u. scitaminea. forty tdfs, 34 newly induced plus six with obvious upregulated expression after infection, were sequenced and validated by rt-pcr analysis. these results demonstrated that the expressio ... | 2011 | 21792273 |
| production and characterization of a glycolipid biosurfactant, mannosylerythritol lipid b, from sugarcane juice by ustilago scitaminea nbrc 32730. | mannosylerythritol lipids (mels) are glycolipid biosurfactants excreted by fungal strains. they show not only excellent surface-active properties but also versatile biochemical actions. ustilago scitaminea nbrc 32730 has been reported mainly to produce a mono-acetylated and di-acylated mel, mel-b, from sucrose as sole carbon source. in order to make biosurfactant production more efficient, we focused our attention on the use of sugarcane juice, one of the most economical resources. the fungal st ... | 2011 | 21737925 |
| differential protein expression in sugarcane during sugarcane-sporisorium scitamineum interaction revealed by 2-de and maldi-tof-tof/ms. | to understand the molecular basis of a specific plant-pathogen interaction, it is important to identify plant proteins that respond to the pathogen attack. two sugarcane varieties, nco376 and ya71-374, were used in this study. by applying 2-dimensional electrophoresis (2-de), the protein expression profile of sugarcane after inoculating with sporisorium scitamineum was analyzed. in total, 23 differentially expressed proteins were identified by maldi-tof-tof/ms. bioinformatics analysis revealed t ... | 2011 | 21822403 |