Publications

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parasitic diseases of marine fish: epidemiological and sanitary considerations.over recent decades, parasitic diseases have been increasingly considered a sanitary and economic threat to mediterranean aquaculture. in order to monitor the distribution of parasites in cultured marine fish from italy and study their pathogenic effects on the host, a three-year survey based on parasitological and histopathological exams was carried out on 2141 subjects from eleven fish species and coming from different farming systems (extensive, intensive inland farms, inshore floating cages, ...200616881387
sharpsnout sea bream (diplodus puntazzo) humoral immune response against the parasite enteromyxum leei (myxozoa).the humoral innate immune response of sharpsnout seabream diplodus puntazzo against the myxozoan enteromyxum leei was studied. enteromyxosis was transmitted by cohabitation and a group of uninfected fish served as control. at 5, 12, 19, 26, 40 and 55 days post-exposure (p.e.), control and recipient fish were sampled to determine the prevalence of infection and some humoral innate immune parameters (antiprotease, antitumoral and peroxidase activities). prevalence of infection was high from day 12 ...200717475509
parasite communities of adriatic cage-reared fish.from june 2001 to march 2002, 7 semi-offshore facilities in the adriatic sea rearing sea bass dicentrarchus labrax, sea bream sparus aurata, sharpsnout bream diplodus puntazzo and red sea bream pagellus bogaraveo aged 1+, were monitored for the presence of protozoan and metazoan parasites. obtained data sets of abundance and prevalence were used to evaluate the structure and dynamic of infra- and component parasite communities. in all hosts, except the red sea bream, dominant parasites were mono ...200515900691
cholecystokinin: how many functions? observations in seabreams.a short overview on the regional distribution of the gastro-intestinal peptide hormone cholecystokin (cck) in fish is presented. in particular, the results of molecular and immunological studies on seabreams, diplodus puntazzo and diplodus sargus, are reported, which, by demonstrating cck in the hindgut, open new questions regarding the functional role of this hormone in that part of the intestine. the putative involvement of hindgut cck in the feedback control of digestive processes was tested ...201424631546
the sex-specific transcriptome of the hermaphrodite sparid sharpsnout seabream (diplodus puntazzo).teleosts are characterized by a remarkable breadth of sexual mechanisms including various forms of hermaphroditism. sparidae is a fish family exhibiting gonochorism or hermaphroditism even in closely related species. the sparid diplodus puntazzo (sharpsnout seabream), exhibits rudimentary hermaphroditism characterized by intersexual immature gonads but single-sex mature ones. apart from the intriguing reproductive biology, it is economically important with a continuously growing aquaculture in t ...201425099474
environmental marine pathogen isolation using mesocosm culture of sharpsnout seabream: striking genomic and morphological features of novel endozoicomonas sp.aquaculture is a burgeoning industry, requiring diversification into new farmed species, which are often at risk from infectious disease. we used a mesocosm technique to investigate the susceptibility of sharpsnout seabream (diplodus puntazzo) larvae to potential environmental pathogens in seawater compared to control borehole water. fish exposed to seawater succumbed to epitheliocystis from 21 days post hatching, causing mortality in a quarter of the hosts. the pathogen responsible was not chla ...201526639610
host-parasite relationship of ceratomyxa puntazzi n. sp. (myxozoa: myxosporea) and sharpsnout seabream diplodus puntazzo (walbaum, 1792) from the mediterranean with first data on ceratomyxid host specificity in sparids.sparidae are economically important fishes to both, fisheries and aquaculture in the mediterranean. species diversification is an important strategy for the development of mediterranean aquaculture. one of the species recently introduced is the sharpsnout seabream diplodus puntazzo (walbaum, 1792). during a parasitological study of fish from the gulf of valencia and the mar menor (spain), myxozoan spores belonging to the genus ceratomyxa were found in the gall bladder of d. puntazzo. a morpholog ...201121680098
myxozoan infections in mediterranean mariculture.fish mariculture has dramatically expanded in recent years in mediterranean countries. in this scenario, several pathological problems have logically arisen and parasitological etiologies are increasingly being reported, either as primary or secondary pathogens. myxozoa is the most diverse and economically important group of fish parasites, and several species are known to cause or contribute to losses in mariculture. species of the genus enteromyxum currently constitute the most serious parasit ...200616881390
antiparasitic and immunomodulatory effect of innovative treatments against myxobolus sp. infection in diplodus puntazzo.the potential antiparasitic and immunomodulatory effect of three treatments against myxosporean parasites on the innate immune system of sharpsnout sea bream (diplodus puntazzo) was investigated. fish naturally infected with myxobolus sp. (bivalvulida/platysporina), a histozoic parasite mainly affecting the renal interstitial tissue, were treated by oral administration of a combination of salinomycin with amprolium, origanum essential oil or fumagillin in a small-scale field trial. various leuco ...200516169152
digestive enzyme activities in larvae of sharpsnout seabream (diplodus puntazzo).the ontogenesis and specific activities of pancreatic and intestinal enzymes were investigated in sharpsnout sea bream, diplodus puntazzo, during larval development until the end of weaning on day 50. the green-water technique was carried out for larval rearing in triplicate. trypsin was first detected as early as hatching and sharply increased related to age and exogenous feeding until day 25, but a sharp decrease was observed towards the end of the experiment. amylase was determined 2 days aft ...200717651999
ontogeny of the digestive tract in sharpsnout sea bream diplodus puntazzo (cetti, 1777).the ontogeny of the digestive tract was studied histologically and histochemically in sharpsnout sea bream diplodus puntazzo from hatching (0 dah, days after hatching) until day 57 (57 dah). at hatching, the digestive tract appeared as a histologically undifferentiated straight tube lying dorsally to the yolk sac. when the mouth opened at 3 dah, the digestive tract was differentiated into buccopharynx, oesophagus, incipient stomach and intestine. the pancreas, liver and gall bladder were also di ...200818581279
organogenesis of exocrine pancreas in sharpsnout sea bream (diplodus puntazzo) larvae: characterization of trypsin expression.the ontogeny and differentiation stages of digestive systems related with trypsin expression in larvae of sharpsnout sea bream, diplodus puntazzo, were investigated from hatching to 40 dah (days after hatching), and total lengths and weights of larvae were determined. histologic and enzymatic techniques were used to explain the functional development of the pancreas including trypsin activity. the pancreas was identified as a compact structure located in the region slightly posterior to the live ...201020077135
ontogeny and distribution of cholecystokinin-immuno reactive cells in the digestive tract of sharpsnout sea bream, diplodus puntazzo (cetti, 1777), during larval development.the appearance and regional distribution of cholecystokinin-immuno reactive cells (cck-ir) in the developing gut of larval diplodus puntazzo were studied by means of immunohistochemistry, with the aim of understanding the role of this peptide hormone in the acquisition of digestive capacity. immunohistochemical reaction showed cck-ir cells from 10 days after hatching (dah), near the pyloric sphincter and past the first bend in the midgut, as well as in the hindgut. at 25 dah cck-ir cells were sc ...201020619264
chronic ulcerative dermatopathy in cultured marine fishes. comparative study in sharpsnout sea bream, diplodus puntazzo (walbaum).chronic ulcerative dermatopathy (cud) also known as chronic erosive dermatopathy, hole-in-the-head, head and lateral line erosion syndrome (hlle) and lateral line depigmentation (lld) is a chronic disease of unknown aetiology that affects the lateral line canals of the head and the trunk of various fish species. it has been described only in freshwater species although there are reports that it also affects marine fish. here, we describe the disease in cultured sharpsnout sea bream using histolo ...201121545440
species specificity in the magnitude and duration of the acute stress response in mediterranean marine fish in culture.the aim of the present study was to examine the species-specific stress response for seven mediterranean fishes in culture. also, to evaluate the method of measuring free cortisol concentration in the rearing water as a non-invasive and reliable indicator of stress in marine species, of aquaculture importance. gilthead sea bream, sparus aurata (sparidae); common dentex, dentex dentex (sparidae); common pandora, pagellus erythrinus (sparidae); sharpsnout sea bream, diplodus puntazzo (sparidae); d ...201121712040
aroclor 1254 inhibits the chemiluminescence response of peritoneal cavity cells from sharpsnout sea bream (diplodus puntazzo).chronic exposure to polychlorinated biphenyls (pcbs) affect the immune system of fish and could lead to a decreased disease resistance. the effects of aroclor 1254, pcb mixtures, on the diplodus puntazzo innate immunity were examined by assaying the zymosan stimulated chemiluminescence response (cl) of peritoneal cavity cells (pccs) at various times (1, 24, 48 h and 1-4 weeks) from intraperitoneal injection of the xenobiotic (1 mg kg(-1) body weight). controls were performed by assaying cells fr ...201424945575
pea protein concentrate in diets for sharpsnout sea bream (diplodus puntazzo): effects on growth and health status.four diets for sharpsnout sea bream juveniles (14 g body weight) with four levels of air-processed pea protein concentrate (ppc) (0, 160, 320 and 487 g/kg diet) were tested in triplicate. the experimental diets were isonitrogenous (43% crude protein) and isolipidic (19% ether extract) and the fish were fed to satiation twice a day. after 125 d, fish growth was diminished by the inclusion of ppc. feed conversion did not show significant differences in any treatment. neither the body analyses nor ...201627666681
risk assessment for parasites in cultures of diplodus puntazzo (sparidae) in the western mediterranean: prospects of cross infection with sparus aurata.the sharpsnout seabream diplodus puntazzo is of interest in mediterranean fish farming. disease is an important problem because parasites can spread quickly in culture conditions and fish often develop high parasite burdens. here we assess the risk that documented parasites pose to the sustainability of d. puntazzo farming. this study specifically considers metazoan and protist parasites recorded from wild and farmed d. puntazzo in scientific literature. risk assessment studies involve the ident ...201424893693
first report of edwardsiellosis in cage-cultured sharpsnout sea bream, diplodus puntazzo from the mediterranean.edwardsiella tarda, is a serious bacterial pathogen affecting a broad range of aquaculture fish species. the bacterium has also been reported as a human pathogen, however recent studies have dissociated the fish pathogenic edwardsiella from those isolated from humans by placing them in a new species, e. piscicida. here we report the first case of edwardsiellosis in cultured sharpsnout sea breams, diplodus puntazzo in greece.201526193880
morphometric, molecular and ecological analyses of the parasites of the sharpsnout seabream diplodus puntazzo cetti (sparidae) from the spanish mediterranean: implications for aquaculture.one of the fish species with the highest potential for aquaculture is the sharpsnout seabream, diplodus puntazzo cetti. among other aspects, the development of new fish cultures requires studies of potential pathogens that may compromise survival of the fish in captivity. moreover, both cultured and wild fish can act as sources or reservoirs of pathogens which may negatively affect other well-established cultures. we have studied the parasite fauna of the wild sharpsnout seabream, and monitored ...201524299967
severe mortality in mesocosm-reared sharpsnout sea bream diplodus puntazzo larvae due to epitheliocystis infection.this paper describes severe mortalities recorded in sharpsnout sea bream diplodus puntazzo larvae reared in mesocosms. the mortalities were attributed to epitheliocystis infection. the pathology associated with the disease is described using histological techniques. microscopical examination showed a massive infection of the skin, fins, and oral cavity, with impaired feeding, respiration, and osmoregulation being the most likely cause of death. this is the first report of epitheliocystis disease ...200819062753
ultrastructure of enteromyxum leei (diamant, lom, & dyková, 1994) (myxozoa), an enteric parasite infecting gilthead sea bream (sparus aurata) and sharpsnout sea bream (diplodus puntazzo).the ultrastructure of the developmental stages of the myxozoan enteromyxum leei parasitizing gilthead seabream (sparus aurata) intestine and sharpsnout sea bream (diplodus puntazzo) intestine and gallbladder are described. the earliest stage observed was a small dense trophozoite located among enterocytes. proliferative stages, observed intercellularly in the epithelium of the intestine and gallbladder as well as in the lumen, possessed the typical cell-in-cell configuration throughout their dev ...200818460155
histopathology and cellular response in enteromyxum leei (myxozoa) infections of diplodus puntazzo (teleostei).enteromyxum leei is an intestinal parasite responsible for serious outbreaks in mediterranean sharpsnout sea bream diplodus puntazzo. e. leei infection was experimentally transmitted to healthy d. puntazzo (r) by cohabitation with infected donor fish. haematological changes and histopathological damage were evaluated in relation to the course of infection. the prevalence of infection in r fish was 100% from day 10 post-exposure (p.e.) onwards, and the infection intensity and histopathological da ...200818373973
host switch of lamellodiscus elegans (monogenea: monopisthocotylea) and sparicotyle chrysophrii (monogenea: polyopisthocotylea) between cage-reared sparids.sharpsnout bream (diplodus puntazzo) has been used in adriatic aquaculture for less than a decade, but the decreasing trend of rearing this species will probably result in its complete substitution by more exploited sea bream (sparus aurata). only two facilities still rear both fish species in neighbouring cages in monoculture. a switch of parasites was observed between sparids during monitoring of the gill monogeneans of farmed fish. in wild fish of the adriatic sea, lamellodiscus elegans (mono ...200717187241
life-history- and ecosystem-driven variation in composition and residence pattern of seabream species (perciformes: sparidae) in two mediterranean coastal lagoons.species composition and length-frequency distributions of six sparid fish species were investigated in two central mediterranean coastal lagoons off the western coast of italy: fogliano and caprolace. in the former, the sparid fauna was dominated by the gilt-head seabream (sparus aurata), whereas in caprolace, species composition was more homogeneous across all six species. size structure varied considerably among species: s. aurata, diplodus puntazzo and diplodus vulgaris had a single-cohort st ...200616266729
potential drug (oxytetracycline and oxolinic acid) pollution from mediterranean sparid fish farms.the potential for input of two common antibacterial agents in mediterranean fish farms, oxytetracycline (otc) and oxolinic acid (oa), was estimated from measurements of these drugs in the faecal excretions of two important farmed sparids, gilthead sea bream, sparus aurata and sharpsnout sea bream diplodus puntazzo. oxolinic acid was found to be well absorbed by gilthead sea bream (92%) and sharpsnout sea bream (88%) while the absorption of otc was found to be considerably lower in both species ( ...200415276333
species diversity among the genus monorchis (digenea: monorchiidae) parasitic in marine teleosts: molecular, morphological and morphometrical studies with a description of monorchis blennii n. sp.molecular, morphological and morphometrical studies were conducted on two species of the genus monorchis (monorchiidae), monorchis parvus and monorchis monorchis, collected in different fish hosts from the mediterranean sea. the analysis of internal transcribed spacer 1 sequences of ribosomal dna showed that m. monorchis specimens from parablennius gattorugine were strongly divergent (12.9%) from specimens of this species collected in spondyliosoma cantharus and diplodus puntazzo. this high gene ...200211954908
differences in tissue fatty acid composition between reared and wild sharpsnout sea bream, diplodus puntazzo (cetti, 1777).the fatty acid composition and lipid content of white muscle, liver and mesenteric fat, in reared v. wild sharpsnout sea bream (diplodus puntazzo) were compared. the mesenteric fat index fat weight/body weight) and the lipid contents of both white muscle and liver proved consistently higher in farmed v. wild sharpsnout sea bream (79.0 (se 13.1) v. 38.7 (se 5.1) g/kg, 188.4 (se 30.0) v. 58.2 (se 3.9) g/kg and 27.2 (se 3.7) v. 17.3 (se 1.9) g/kg, respectively). the higher values of linoleic, eicos ...200111737960
understanding myxozoan infection dynamics in the sea: seasonality and transmission of ceratomyxa puntazzi.ceratomyxa puntazzi affects the sharpsnout seabream, diplodus puntazzo, a recently explored aquaculture species in the mediterranean. little is known about the transmission and seasonality of marine myxozoans, although this knowledge is of considerable importance for the design of management strategies for aquaculture. in the present study on c. puntazzi we investigated the potential pathways of transmission as well as the parasite abundance in fish and its density in environmental water samples ...201323747926
ultrastructural aspects of previtellogenic oocyte growth in hermaphrodite sharpsnout seabream, diplodus puntazzo (teleostei, sparidae).this paper describes various aspects of previtellogenic oocyte growth in sharpsnout seabream, diplodus puntazzo , is an important marine culture fish species in the mediterranean. the ultrastructural characteristics of nuclear morphology, nuclear-cytoplasmic ratio and the starting of the follicle envelope formation were described in detail. these cells do not significantly differ from those of the other teleost species. the ultrastructural aspects provide new information on the reproductive biol ...200919584025
energy intake and macronutrient selection in sharpsnout seabream (diplodus puntazzo) challenged with fat dilution and fat deprivation using encapsulated diets.sharpsnout seabream fed pure macronutrient capsules were challenged to fat dilution and fat deprivation in order to investigate the effects of fat level on energy intake regulation and macronutrient selection by fish, as they lack oropharyngeal chemosensory information from the diet. during the control phase, the fish were fed three individually encapsulated macronutrients, from which they composed a diet containing 67.36% protein (p), 19.08% carbohydrates (ch) and 13.57% fat (f), in terms of ma ...200817997462
locomotor, feeding and melatonin daily rhythms in sharpsnout seabream (diplodus puntazzo).sharpsnout seabream is a marine teleost of increasing interest for mediterranean aquaculture, but there is still a lack of information regarding its circadian organization. in this study, we have investigated sharpsnout seabream locomotor activity, feeding and plasma melatonin daily rhythms under a 12:12-h ld cycle, as well as the persistence of locomotor activity circadian rhythmicity under constant light (ll) conditions. when submitted to an ld cycle, most sharpsnout seabream displayed a diurn ...200616682061
oxytetracycline hydrochloride vital labelling revisited: the case of dicentrarchus labrax and diplodus puntazzo.the main objective of this study was to determine the optimal oxytetracycline hydrochloride (otc) dosage for otolith marking in dicentrarchus labrax and diplodus puntazzo. adult reared individuals of these two species were marked with otc intraperitoneal injections at three recommended doses (25, 45 and 60 mg kg(-1) fish) and a blank. fishes were monitored for 36-49 days from when they were marked. the effectiveness of the otolith marking was inversely related to the otc dosage. the injections w ...201121366571
the atlantic-mediterranean transition: discordant genetic patterns in two seabream species, diplodus puntazzo (cetti) and diplodus sargus (l.).sparids are a group of demersal perciform fish of high commercial value, which have experienced an extensive radiation, particularly in the mediterranean, where they occupy a variety of different niches. the present study focuses on two species: diplodus sargus and d. puntazzo, presenting a wide distribution from the mediterranean to the eastern atlantic coasts. they display similar ecological behaviour and are evolutionary closely related. both are highly appreciated in fisheries and d. puntazz ...200515936957
histological and cytological studies on the developing thymus of sharpsnout seabream, diplodus puntazzo.the structure of the developing thymus of the marine teleost, diplodus puntazzo, was studied by light and transmission electron microscopy. the first anlage of the thymus developed by d 20 postfertilisation (p.f.) as a group of undifferentiated cells dorsal to the epithelium of the branchial chamber. the organ increased significantly in size around d 51-66 p.f. and differentiation of cortex and medulla occurred concomitantly. on the basis of their localisation, 4 main types of epithelial cell we ...199910227665
is cryopreservation a homogeneous process? ultrastructure and motility of untreated, prefreezing, and postthawed spermatozoa of diplodus puntazzo (cetti).this study subdivides the cryopreservation procedure for diplodus puntazzo spermatozoa into three key phases, fresh, prefreezing (samples equilibrated in cryosolutions), and postthawed stages, and examines the ultrastructural anomalies and motility profiles of spermatozoa in each stage, with different cryodiluents. two simple cryosolutions were evaluated: 0.17 m sodium chloride containing a final concentration of 15% dimethyl sulfoxide (me(2)so) (cryosolution a) and 0.1 m sodium citrate containi ...200111748933
multiple recurrent evolution of trophic types in northeastern atlantic and mediterranean seabreams (sparidae, percoidei).seabreams are among the most valuable fish, not only for small-scale and semiindustrial fisheries but also for aquaculture throughout the mediterranean. nevertheless, their phylogenetic relationships are not at all clear. the current taxonomy is based solely on trophic morphology and rests on the assumption that each trophic type evolved only once from a less specialized ancestral condition. we analyzed a 486-bp segment of the mitochondrial 16s rdna of all 24 seabream species described for the n ...200010754071
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