| ima genome-f 6: draft genome sequences of armillaria fuscipes, ceratocystiopsis minuta, ceratocystis adiposa, endoconidiophora laricicola, e. polonica and penicillium freii daomc 242723. | the genomes of armillaria fuscipes, ceratocystiopsis minuta, ceratocystis adiposa, endoconidiophora laricicola, e. polonica, and penicillium freii daomc 242723 are presented in this genome announcement. these six genomes are from plant pathogens and otherwise economically important fungal species. the genome sizes range from 21 mb in the case of ceratocystiopsis minuta to 58 mb for the basidiomycete armillaria fuscipes. these genomes include the first reports of genomes for the genus endoconidio ... | 2016 | 27433447 |
| cloning, over-expression and characterization of an alkali-tolerant endo-β-1,4-mannanase from penicillium freii f63. | a glycosyl hydrolase family 5 endo-β-mannanase gene (man5f63) was cloned from penicillium freii f63 and overexpressed in pichia pastoris. man5f63 contained an open reading frame of 1260 bp that encoded a polypeptide of 419 amino acids including a putative 18-residue signal peptide. the recombinant enzyme (rman5f63) was secreted into the culture supernatant to near electrophoretic homogeneity with a high yield (1.1 gl(-1) in flask). its apparent molecular weight was approximately 72.0 kda, 29.0 k ... | 2012 | 22382013 |
| a penicillium freii gene that is highly similar to the beta-ketoacyl synthase domain of polyketide synthase genes from other fungi. | a penicillium freii dna fragment with similarity to the beta-keto-acyl synthase motif of the p. griseofulvum 6-methylsalicylic acid synthase encoding gene (msas) was identified by screening a cosmid library using a part of msas as the probe. two exons of 93 and 849 bp, encoding a predicted polypeptide of 314 amino acids, and a molecular mass of 33.4 kda, were identified (pfks). pfks was transcribed as a 1.6 kbp messenger. a region corresponding to the msas gene encoding essential enzyme activiti ... | 1997 | 9351263 |
| 2d-page examination of mrna populations from penicillium freii mutants deficient in xanthomegnin biosynthesis. | penicillium freii (lund and frisvad 1994) mutants deficient in the synthesis of xanthomegnin were isolated. in vitro translated mrna populations from selected radiation induced mutants and naturally occurring p. freii strains not able to produce xanthomegnin were examined by 2-dimensional polyacrylamide gel electrophoresis (2d-page). specific translation products were absent in mutants and natural isolates unable to produce xanthomegnin metabolites. one mutant (tsm 73) did not produce several of ... | 1996 | 8817920 |
| transformation of penicillium freii and a rapid pcr screening procedure for cotransformation events. | a strain of penicillium freii was transformed to hygromycin b resistance using the pan7-1 plasmid. the transformation frequency was approximately 100 transformants per microgram dna per 1 x 10(7) protoplasts. southern blotting revealed different types of integration into the genome of p.freii. when non-selectable plasmid dna was added together with pan7-1, this resulted in a cotransformation frequency of more than 60%. the cotransformation frequency was estimated using a facile dna extraction me ... | 1996 | 8817919 |