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mitochondrial dna polymorphism in populations of siberian and european roe deer (capreolus pygargus and c. capreolus).we have amplified and sequenced 679 nucleotides of the mitochondrial dna control-region in 45 siberian (capreolus pygargus) and european (c. capreolus) roe deer from two localities in russia and seven in italy. average interspecific sequence divergence was 4.9%. six different haplotypes were found in siberian roe deer, and 14 haplotypes in alpine european roe deer. a population of the endemic italian subspecies c. c. italicus was monomorphic bearing a single haplotype with one unique nucleotide ...19989618912
[variability of multilocus dna markers in populations of the siberian (capreolus pygargus pall.) and european (c. capreolus l.) roe deer].two types of nuclear dna markers, m13 minisatellites and rapd, were used to examine intraspecific and interspecific variation in closely related roe deer species, capreolus capreolus l. and c. pygargus pall. the roe deer populations studied were highly polymorphic for minisatellite dna markers (s = 0.12-0.36). heterozygosities of the rapd loci were 0.185 (russian far east), 0.145, 0.131, 0.088 (cis-ural), and 0.06 (france). they correlated with karyotypic variation of b chromosomes (r = 0.975, p ...200011094770
[quantitative analysis of genetic parameters in populations of european (capreolus capreolus l.) and siberian (capreolus pygargus pall.) roe deer with rapd markers].a technique for obtaining unbiased estimates of genetic parameters (allelic frequencies of rapd loci, heterozygosity (h), wright's f statistic, and nei's genetic distances) in populations of the european (capreolus capreolus l.) and siberian (capreolus pygargus pall.) roe deer is presented. the technique employs jackknifing and multiple comparative analysis based on a modified holmes's procedure for bonferroni's test. it was demonstrated that samples from local groups of roe deer in the trans-ur ...200212138780
[morphological peculiarities of terminal placental villi in capreolus capreolus pygargus].this investigation was aimed at the study of peculiarities of placentation in siberian roe (capreolus capreolus pygargus)--one of the representatives of the cervidae family which is characterized by an embryonic diapause. using histological and morphometric methods, the main structural-functional regularities of the organization of roe placenta were determined at different stages of gestation, that included the formation of placenta at later stages of gestation, as compared with other ruminants, ...200516400918
mitochondrial dna distinction of northeastern china roe deer, siberian roe deer, and european roe deer, to clarify the taxonomic status of northeastern china roe deer.partial sequences of the mitochondrial control region of northeastern china roe deer were analyzed to determine the degree of genetic diversity. fourteen haplotypes were observed. the haplotype diversity was high (h = 0.872), nucleotide diversity was medium (p ( i ) = 0.0108), and the average tamura-nei nucleotide distance among them was 1.9%, indicating that genetic diversity of roe deer from northeastern china was relatively high and that the effective population size was large historically. t ...200717219081
species and sex identification of the korean goral (nemorhaedus caudatus) by molecular analysis of non-invasive samples.korean long-tailed goral (nemorhaedus caudatus) is one of the most endangered species in south korea. however, detailed species distribution and sex ratio data on the elusive goral are still lacking due to difficulty of identification of the species and sex in the field. the primary aim of this study was to develop an economical pcr-rflp method to identify species using invasive or non-invasive samples from five korean ungulates: goral (n. caudatus), roe deer (capreolus pygargus), feral goat (ca ...200818679054
reconstruction of the putative cervidae ancestral karyotype by chromosome painting of siberian roe deer (capreolus pygargus) with dromedary probes.the siberian roe deer (capreolus pygargus) is one of a few deer species presumably preserving the ancestral cervid karyotype. the comparative genomic data of the siberian roe deer are critical for our understanding of the karyotypic relationships within artiodactyls. we have established chromosomal homologies between the siberian roe deer and the dromedary (camelus dromedarius) by cross-species chromosome painting with dromedary chromosome-specific painting probes. dromedary chromosome paints de ...201020413959
genetic diversity and genetic structure of the siberian roe deer (capreolus pygargus) populations from asia.the roe deer, capreolus sp., is one of the most widespread meso-mammals of palearctic distribution, and includes two species, the european roe deer, c. capreolus inhabiting mainly europe, and the siberian roe deer, c. pygargus, distributed throughout continental asia. although there are a number of genetic studies concerning european roe deer, the siberian roe deer has been studied less, and none of these studies use microsatellite markers. natural processes have led to genetic structuring in wi ...201526282405
genotyping of capreolus pygargus fossil dna from denisova cave reveals phylogenetic relationships between ancient and modern populations.the extant roe deer (capreolus gray, 1821) includes two species: the european roe deer (c. capreolus) and the siberian roe deer (c. pygargus) that are distinguished by morphological and karyotypical differences. the siberian roe deer occupies a vast area of asia and is considerably less studied than the european roe deer. modern systematics of the siberian roe deer remain controversial with 4 morphological subspecies. roe deer fossilized bones are quite abundant in denisova cave (altai mountains ...201121897868
contrasting origin of b chromosomes in two cervids (siberian roe deer and grey brocket deer) unravelled by chromosome-specific dna sequencing.b chromosomes are dispensable and variable karyotypic elements found in some species of animals, plants and fungi. they often originate from duplications and translocations of host genomic regions or result from hybridization. in most species, little is known about their dna content. here we perform high-throughput sequencing and analysis of b chromosomes of roe deer and brocket deer, the only representatives of cetartiodactyla known to have b chromosomes.201627516089
transcription of a protein-coding gene on b chromosomes of the siberian roe deer (capreolus pygargus).most eukaryotic species represent stable karyotypes with a particular diploid number. b chromosomes are additional to standard karyotypes and may vary in size, number and morphology even between cells of the same individual. for many years it was generally believed that b chromosomes found in some plant, animal and fungi species lacked active genes. recently, molecular cytogenetic studies showed the presence of additional copies of protein-coding genes on b chromosomes. however, the transcriptio ...201323915065
prevalence of antibody to and dna of lawsonia intracellularis in samples from wild animals in korea.we evaluated the prevalence of lawsonia intracellularis infection in three wild animal species in korea; the korean water deer ( hydropotes inermis ), siberian roe deer ( capreolus pygargus ), and raccoon dogs ( nyctereutes procyonoides ). we collected 136 sera and 109 fecal samples from individuals in 10 wildlife rescue and conservation centers. serum samples were tested for anti- l. intracellularis antibodies using a blocking enzyme-linked immunosorbent assay (belisa), and fecal samples were s ...201627487235
description and life-cycle of taenia lynciscapreoli sp. n. (cestoda, cyclophyllidea).a new species of tapeworm, taenia lynciscapreoli sp. n. (cestoda, cyclophyllidea), is described from the eurasian lynx (lynx lynx), the main definitive host, and the roe deer (capreolus capreolus and capreolus pygargus), the main intermediate hosts, from finland and russia (siberia and the russian far east). the new species was found once also in the wolf (canis lupus) and the eurasian elk/moose (alces alces), representing accidental definitive and intermediate hosts, respectively. the conspecif ...201627199592
effects of interspecific interaction-linked habitat factors on moose resource selection and environmental stress.resource selection of herbivores is a complex ecological process that operates in relation to biological or non-biological factors, which may affect the feeding and movement, and subsequently their spatial distribution and environmental stress. here, we estimated moose (alces alces cameloides) resource selection for habitat variables and the effect of interspecific interactions related to roe deer (capreolus pygargus bedfordi) on its population distribution and environmental stress in the khinga ...201728128311
serological survey of avian influenza virus infection in non-avian wildlife in xinjiang, china.we conducted a serological survey to detect antibodies against avian influenza virus (aiv) in gazella subgutturosa, canis lupus, capreolus pygargus, sus scrofa, cervus elaphus, capra ibex, ovis ammon, bos grunniens and pseudois nayaur in xinjiang, china. two hundred forty-six sera collected from 2009 to 2013 were assayed for antibodies against h5, h7 and h9 aivs using hemagglutination inhibition (hi) tests and a pan-influenza competitive elisa. across all tested wildlife species, 4.47 % harbored ...201626733295
cytogenetic description of the amazonian brown brocket mazama nemorivaga (artiodactyla, cervidae).the amazonian brown brocket mazama nemorivaga (cuvier, 1817) is a small to medium-sized deer from the amazon rainforest and ecotones. the first karyotype described was 2n=67 to 69 + 2-7 b and fn= 69-72, in which all chromosomes were acrocentric and the x chromosome was the only submetacentric chromosome. however, important aspects of the species chromosome evolution were not resolved because of the lack of information on chromosome banding. the g-banding pattern of mazama nemorivaga karyotype sh ...201324260687
diversity of formyltetrahydrofolate synthetase genes in the rumens of roe deer (capreolus pygargus) and sika deer (cervus nippon) fed different diets.reductive acetogenesis by homoacetogens represents an alternative pathway to methanogenesis to remove metabolic hydrogen during rumen fermentation. in this study, we investigated the occurrence of homoacetogen in the rumens of pasture-fed roe deer (capreolus pygargus) and sika deer (cervus nippon) fed either oak-leaf-based (tannin-rich, 100 mg/kg dried matter), corn-stover-based, or corn-silage-based diets, by using formyltetrahydrofolate synthetase (fthfs) gene sequences as a marker. the divers ...201727819479
immunohistochemical localization of gabaergic key molecules in the main olfactory bulb of the korean roe deer, capreolus pygargus.gamma-amino butyric acid (gaba) negatively regulates the excitatory activity of neurons and is a predominant neurotransmitter in the nervous system. the olfactory bulb, the main center in the olfactory system, is modulated by inhibitory interneurons that use gaba as their main neurotransmitter. the present study aimed to evaluate gabaergic transmission in the main olfactory bulb (mob) of the korean roe deer (capreolus pygargus) by examining the immunohistochemical localization of gabaergic key m ...201526115600
bacteria and methanogens differ along the gastrointestinal tract of chinese roe deer (capreolus pygargus).the current study provides the insight into the bacteria in the gastrointestinal tract (git) and methanogens presented in the rumen and cecum of the chinese roe deer (capreolus pygargus). the ruminal, ileal, cecal, and colonic contents, as well as feces, were obtained from each of the three, free-range, roe deer ingesting natural pasture after euthanasia. for the bacterial community, a total of 697,031 high-quality 16s rrna gene sequences were generated using high-throughput sequencing, and assi ...201425490208
histological and lectin histochemical studies on the olfactory mucosae of the korean roe deer, capreolus pygargus.the morphological features of the olfactory mucosae of korean roe deer, capreolus pygargus, were histologically studied using the ethmoid turbinates containing the olfactory mucosae from six roe deer (male, 2-3 years old). the ethmoid turbinates were embedded in paraffin, and histochemically evaluated in terms of the mucosal characteristics. lectin histochemistry was performed to investigate the carbohydrate-binding specificity on the olfactory mucosa. lectins, including triticum vulgaris wheat ...201525480445
new record of lipoptena fortisetosa (diptera: hippoboscidae) collected from siberian roe deer on jeju island, korea.lipoptena deer keds (diptera: hippoboscidae) are blood-sucking ectoparasites of mammals, and only one species, lipoptena cervi (l.), has previously been reported from the republic of korea. a study of siberian roe deer capreolus pygargus pallas on jeju island, south of the korean mainland, led to the discovery of a second species of deer ked, lipoptena fortisetosa maa, for korea. in total, 518 deer keds were collected from 19 adult and juvenile deer examined from may to october 2012. the infesta ...201324180125
a morphological study of the vomeronasal organ and the accessory olfactory bulb in the korean roe deer, capreolus pygargus.the vomeronasal organ (vno) and accessory olfactory bulb (aob) of the korean roe deer (capreolus pygargus) were studied histologically to evaluate their morphological characteristics. grossly, the vno, encased by cartilage, has a paired tubular structure with a caudal blind end and a rostral connection through incisive ducts on the hard palate. in the vno, the vomeronasal sensory epithelium (vse) consists of galectin-3-positive supporting cells, protein gene product (pgp) 9.5-positive receptor c ...201424055195
[analysis of the variability of the control region and cytochrome b gene of mtdna of capreolus pygargus pall].analysis the of cytochrome b gene (1140 b.p.) polymorphism and polymorphism of 79 specimens of the control region (934 b.p.) of mtdna of the siberian roe deer capreolus pygargus from 23 regions of russia and kazakhstan has been carried out. the combined alignment of two fragments ofmitochondrial dna revealed 36 haplotypes, which are distributed in three haplogroups. the net distance was 0.8, 1.3, and 1.3%. the specific pattern of haplogroups was described for roe deer of each region (ural, trans ...201622117417
[polymorphism of a short fragment of the mitochondrial genome control region (d-loop) in the siberian roe deer capreolus pygargus pallas, 1771 (artiodactyla, cervidae) from the russian far east].genetic diversity of siberian roe deer capreolus pygargus, 1771 from the russian far east was studied based on polymorphism analysis of a mtdna control region fragment (390 bp). three phylogenetic lines were found in the animals examined. the trend for change of haplotypes of different phylogroups was shown to occur from north to south in sikhote-alin. the haplotype distribution of siberian roe deer in the russian far east correlated with data on morphologiocal variability.201020583604
[karyotype of siberian roe-deer (capreolus pygargus pall.) from the northern border of the areal]. 19989644585
[the social organization, behavior and ecological parameters of a migrating population of siberian roe deer (capreolus pygargus pall.)].data obtained in 1988-1994 during a long-term study of the ecology and behavior of a seasonally migrating siberian roe population in amur province are reviewed. aerial surveillance of 24 roe marked with transmitters was used for tracing their migration routes under normal conditions and in the homing experiment. it was found that the routes of roe migrations can be as long as 200 km. these routes, as well as summer and winter territories and the sites where roe cross the rivers, remain unchanged ...20167894315
intraspecific variation of the interparietal suture closure in siberian roe deer capreolus pygargus from jeju island.the sequence of cranial suture closure among cervids is reported to be generally species-specific and highly conservative within species. on the other hand, it is known that intraspecific variation often exists to some extent in other mammalian taxa. here we studied the cranial suture closures of capreolus pygargus from jeju island and compared it with other cervid species. we found that the timing of the interparietal suture closure is highly variable within c. pygargus. capreolus capreolus sim ...201729109355
dominance of the enterocytozoon bieneusi genotype beb6 in red deer (cervus elaphus) and siberian roe deer (capreolus pygargus) in china and a brief literature review.enterocytozoon bieneusi is the most frequently diagnosed microsporidian species in humans and is also found in a wide range of animals. it is considered to be an important but neglected zoonotic pathogen. with the development of deer bred in captivity, the number of deer has been increasing in recent years in china and there are more people involved in this work. the aims of this study were to determine prevalence and genotypes of e. bieneusi in red deer (cervus elaphus) and siberian roe deer (c ...201729267159
branching patterns of the aortic arch in the siberian roe deer (capreolus pygargus pallas, 1771).this study examined the branching pattern of the aortic arch (aa) and its major branches in the siberian roe deer (capreolus pygargus pallas, 1771) from south korea. a total of eight of the nine expected types, based on the branching site and bilateral levels of the costocervical trunk (cct) and subclavian artery (sb), were observed in the arterial silicone casts of 35 deer (16 males, 19 females). this deer has no typical type. the three most common types were present in 28.6%, 25.7%, and 20.0% ...201729199212
glycan diversity in the vomeronasal organ of the korean roe deer, capreolus pygargus: a lectin histochemical study.glycans in the epithelium play an important role in cell-to-cell communication and adhesion. no detailed evaluation of glycoconjugates in the vomeronasal organs (vno) of the roe deer has been published previously. the aim of this study was to characterize glycan epitopes in the vomeronasal sensory epithelium (vse) and non-sensory epithelium (vnse) using lectin histochemistry. glycan epitopes identified by lectin histochemistry were grouped as follows: n-acetylglucosamine (s-wga, wga, bsl-ii, dsl ...201729029805
immunohistochemical study of arginases 1 and 2 in the olfactory bulbs of the korean roe deer, capreolus pygargus.arginases are enzymes of the urea cycle that catalyze the hydrolysis of l-arginine to ornithine and urea. the enzymes are core components of the arginine-ornithine-glutamate-γ-amino butyric acid pathway of the central nervous system. in the present study, we immunohistochemically determined the localization of arginase 1 and 2 in the olfactory bulb (ob) of the roe dear (capreolus pygargus). reverse transcription pcr revealed that the mrnas encoding both arginase 1 and 2 were expressed in the ob. ...201728886869
genetic diversity and phylogeography of siberian roe deer, caproulus pygargus, in central and peripheral populations.current understanding of phylogeographical structure and genetic diversity of siberian roe deer remains limited mainly due to small sample size and/or low geographical coverage in previous studies. published data suggest at least two phylogroups: western (ural mountains and western siberia) and eastern (east from lake baikal, including the korean peninsula), but their phylogenetic relationship remains unclear. combined sequences of cytochrome b (1140 bp) and the mtdna control region (963 bp) wer ...201628725397
novel variants of the newly emerged anaplasma capra from korean water deer (hydropotes inermis argyropus) in south korea.anaplasma spp. are tick-borne gram-negative obligate intracellular bacteria that infect humans and a wide range of animals. anaplasma capra has emerged as a human pathogen; however, little is known about the occurrence and genetic identity of this agent in wildlife. the present study aimed to determine the infection rate and genetic profile of this pathogen in wild animals in the republic of korea.201931345253
molecular detection and identification of tick-borne bacteria and protozoans in goats and wild siberian roe deer (capreolus pygargus) from heilongjiang province, northeastern china.small ruminants are important hosts for various tick species and tick-associated organisms, many of which are zoonotic. the aim of the present study was to determine the presence of tick-borne protozoans and bacteria of public health and veterinary significance in goats and wild siberian roe deer (capreolus pygargus) from heilongjiang province, northeastern china.201931196180
influence of tree thinning on the abundance of mammals in a japanese larch larix kaempferi plantation.we examined the influence of tree thinning on the abundance of mammals in a japanese larch larix kaempferi plantation in mt gariwang, pyeongchang, south korea. in 2014 and 2015, from april to october, we recorded the presence of tracks as a proxy for abundance, for the following seven mammalian species: korean hare lepus coreanus, raccoon dog nyctereutes procyonoides, siberian weasel mustela sibirica, eurasian badger meles meles, water deer hydropotes inermis, roe deer capreolus pygargus, and wi ...201730460053
estimating abundance of siberian roe deer using fecal-dna capture-mark-recapture in northeast china.it is necessary to estimate the population abundance of deer for managing their populations. however, most estimates are from high-density populations inhabiting the forests of north america or europe; there is currently a lack of necessary knowledge regarding low-density deer populations in different forest habitats. in this article, we used fecal dna based on the capture-mark-recapture method to estimate the population abundance of siberian roe deer (capreoluspygargus) in liangshui national na ...202032635344
various branching patterns of the aortic arch in the siberian roe deer (capreolus pygargus, pallas, 1771) on jeju island.this study examined and compared the branching pattern of the aortic arch (aa) and its major branches in the siberian roe deer (capreolus pygargus) on jeju island (jeju roe deer [jrd]) with those in the roe deer of the korean peninsula (mainland roe deer [mrd]). seven of the nine expected types was observed in the arterial silicone casts of 29 deer (10 males, 19 females). the jrd was identical to the mrd in that absence of the typical pattern; however, the main three pattern types differed betwe ...201930568051
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