| molecular and genetic structure of multiple hemoglobins in the eelpout, zoarces vivparus l. | six major hemoglobin components are present in the teleostean fish zoarces viviparus l. biochemical characterization has led to a molecular model for the polypeptide chain composition of the individual hemoglobins. only three different chains are involved. they are determined by three different structural loci, as indicated by the genetic variation of the electrophoretic hemoglobin pattern observed in natural populations. hemoglobins occur that, despite identical chain compositions, have differe ... | 1975 | 1180878 |
| cholinergic and adrenergic influence on the teleost heart in vivo. | the tonical cholinergic and adrenergic influence on the heart rate was investigated in vivo in seven species of marine teleosts (pollack, pollachius pollachius; cuckoo wrasse, labrus mixtus; ballan wrasse, labrus berggylta; five-bearded rockling, ciliata mustela; tadpole fish, raniceps raninus; eel-pout, zoarces viviparus and short-spined sea scorpion, myoxocephalus scor pius) during rest and, in two of the species (p. pollachius and l. mixtus), also during moderate swimming exercise in a blazka ... | 1987 | 3582588 |
| different stability of posttranslationally modified brain microtubules isolated from cold-temperate fish. | microtubule proteins were isolated by a temperature-dependent assembly-disassembly method from brain tissue of for cold-temperature fish; one fresh water fish (oncorhynchus mykiss), and three marine fish (labrus berggylta, zoarces viviparus and gadus morhua). the alpha-tubulins from all four fish species were acetylated. the alpha-tubulins from the marine fish were composed of a mixture of tyrosinated and detyrosinated tubulin, while the fresh water fish tubulin only reacted with an antibody aga ... | 1994 | 8028593 |
| effects of nonylphenol and 17 beta-oestradiol on vitellogenin synthesis, testicular structure and cytology in male eelpout zoarces viviparus. | nonylphenol has been found to be oestrogenic in fish and may influence the reproductive system of male fish. in the present study, the effects of low (10 microg g-1 week-1) and high (100 microg g-1 week-1) doses of nonylphenol and of 17 beta-oestradiol on the synthesis of vitellogenin and on testicular structure and cytology were investigated in male eelpout zoarces viviparus during active spermatogenesis (may) and late spermatogenesis (june). twenty-five days after injection, a significant dose ... | 1998 | 9405301 |
| vitellogenin in zoarces viviparus: purification, quantification by elisa and induction by estradiol-17 beta and 4-nonylphenol. | vitellogenin was isolated by gel filtration and ion-exchange chromatography from plasma of estradiol-treated male zoarces viviparus. the purification of vitellogenin was followed through each step by native polyacrylamide gelelectrophoresis (page). the purified vitellogenin was used to raise anti-vitellogenin antibodies in rabbits. the specificity of the affinity purified antibody raised against vitellogenin was assessed by western blot analysis. no crossreactivity was observed with plasma from ... | 1998 | 9827029 |
| temperature-dependent expression of cytochrome-c oxidase in antarctic and temperate fish. | seasonal acclimation versus permanent adaptation to low temperatures leads to a differential response in the expression of cytochrome-c oxidase (cco) in temperate and antarctic eelpouts. although eurythermal eelpout from the north sea (zoarces viviparus) displayed a cold-induced rise of cco activity in white muscle, enzyme activity in the cold stenothermal antarctic eelpout pachycara brachycephalum failed to reflect such a compensatory increase. in antarctic eelpout, cco activity correlates with ... | 1999 | 10444558 |
| intersex in feral marine and freshwater fish from northeastern germany. | a histopathological assessment of the gonad of male fish was performed as part of biological field studies carried out in coastal waters and small rivers in mecklenburg-western pomerania, northeastern germany. in the marine environment the eelpout (zoarces viviparus) was selected as sentinel species. the three spined stickleback (gasterosteus aculeatus) and perch (perca fluviatilis) were chosen at freshwater locations. histopathology of the testis revealed the presence of intersexuality in speci ... | 2002 | 12408630 |
| mitochondrial proliferation in the permanent vs. temporary cold: enzyme activities and mrna levels in antarctic and temperate zoarcid fish. | adjustments in mitochondrial properties and capacities are crucial in acclimatization to seasonal cold and in evolutionary cold adaptation of marine ectotherms. although long-term compensatory increments in aerobic capacity of fish tissues have frequently been described in response to cold, much less is known about transitional phases and gene expression patterns involved. we investigated the time course of adjustment to acute cold in liver of eurythermal eelpout zoarces viviparus. whereas citra ... | 2003 | 12907412 |
| dna damage in eelpout (zoarces viviparus) from göteborg harbour. | the relationship between dna damage and the exposure of marine organisms to environmental contaminants was examined in the göteborg harbour area. this research is part of a wider ecotoxicological study planned to evaluate the biological impact of chemical contamination in the river göta estuary, following a bunker oil (10-100 tonnes) spill occurred in june 2003. here we present data on the dna strand breaks derived using the comet assay and the presence of apoptotic cells using the diffusion ass ... | 2004 | 15288551 |
| effects of redox cycling compounds on dt diaphorase activity in the liver of rainbow trout (oncorhynchus mykiss). | background: dt diaphorase (dtd; nad(p)h:quinone oxidoreductase; ec 1.6.99.2) catalyses the two electron reduction of quinones, thus preventing redox cycling and consequently quinone dependent production of reactive oxygen species. in rat and mouse, a wide range of chemicals including polyaromatic hydrocarbons, azo dyes and quinones induces dtd. bifunctional compounds, such as beta-naphthoflavone (beta-nf) and benzo(a)pyrene (b(a)p), induce dtd together with cyp1a and phase ii enzymes by a mechan ... | 2005 | 15871734 |
| oxidative damage in eelpout (zoarces viviparus), measured as protein carbonyls and tbars, as biomarkers. | during 2002-2003 a dredging campaign was undertaken in göteborg harbor, sweden, to widen and deepen shipping lanes. a bunker oil spill occurred in the harbor in july 2003, thereby further exasperating the situation for marine life. eelpout, zoarces viviparus, was used as a sentinel species to monitor the impact of these events. here, we have investigated the effects on two liver parameters, lipid peroxidation and protein carbonylation, which can serve as biomarkers for oxidative stress. lipid pe ... | 2005 | 15917092 |
| temperature-dependent protein synthesis capacities in antarctic and temperate (north sea) fish (zoarcidae). | for an evaluation of effects of seasonal cold acclimation and evolutionary cold adaptation on protein synthesis capacity, the protein synthesis apparatus was isolated from the gills and white muscle of antarctic eelpout pachycara brachycephalum and north sea eelpout zoarces viviparus. both species had been acclimated to 0 degrees c (control) and 5 degrees c (antarctic) and 5 degrees c and 10 degrees c (north sea control). the translational capacities of the protein synthesis machineries were det ... | 2005 | 15939780 |
| effects of dredging in göteborg harbor, sweden, assessed by biomarkers in eelpout (zoarces viviparus). | we used a battery of biomarkers in fish to study the effects of the extensive dredging in göteborg harbor situated at the river göta alv estuary, sweden. eelpout (zoarces viviparus) were sampled along a gradient into göteborg harbor, both before and during the dredging. biomarker responses in the eelpout before the dredging already indicated that fish in göteborg harbor are chronically affected by pollutants under normal conditions compared to those in a reference area. however, the results duri ... | 2005 | 16152967 |
| developmental disorders in larvae of eelpout (zoarces viviparus) from german and swedish baltic coastal waters. | pregnant eelpout were collected for several years at german and swedish baltic coastal stations with different impact of pollutants and the prevalence of fry abnormalities compared between sites. mortality of apparently normal developed larvae was observed with high variability in both german and swedish sites. malformation of larvae was a prominent disorder at all german stations with a prevalence ranging from about 50% up to a maximum of 90% in almost all analysed samples. at the swedish stati ... | 2006 | 16364374 |
| effects of seasonal and latitudinal cold on oxidative stress parameters and activation of hypoxia inducible factor (hif-1) in zoarcid fish. | acute, short term cooling of north sea eelpout zoarces viviparus is associated with a reduction of tissue redox state and activation of hypoxia inducible factor (hif-1) in the liver. the present study explores the response of hif-1 to seasonal cold in zoarces viviparus, and to latitudinal cold by comparing the eurythermal north sea fish to stenothermal antarctic eelpout (pachycara brachycephalum). hypoxic signalling (hif-1 dna binding activity) was studied in liver of summer and winter north sea ... | 2007 | 17579869 |
| two new antarctic gyrodactylus species (monogenoidea): description and phylogenetic characterization. | two new species of gyrodactylus nordmann, 1832 (gyrodactylidae, monogenoidea) are described, i.e., gyrodactylus coriicepsi n. sp. from gills of black rockcod, notothenia coriiceps richardson, 1844, and gyrodactylus nudifronsi n. sp. from gills of gaudy notothen, lepidonotothen nudifrons lönnberg, 1905, collected in waters surrounding the south shetland islands, antarctica. gyrodactylus coriicepsi n. sp. is characterized by a long, narrowing membrane of the ventral bar and a marginal hook sickle ... | 2009 | 19413367 |
| hypercapnia induced shifts in gill energy budgets of antarctic notothenioids. | mechanisms responsive to hypercapnia (elevated co(2) concentrations) and shaping branchial energy turnover were investigated in isolated perfused gills of two antarctic notothenioids (gobionotothen gibberifrons, notothenia coriiceps). branchial oxygen consumption was measured under normo- versus hypercapnic conditions (10,000 ppm co(2)) at high extracellular ph values. the fractional costs of ion regulation, protein and rna synthesis in the energy budgets were determined using specific inhibitor ... | 2010 | 19834716 |
| twenty years of elemental analysis of marine biota within the german environmental specimen bank--a thorough look at the data. | as one component of the german ecological environment observation, the environmental specimen bank program was initiated in the mid-1980s. under the program, representative specimens of marine, fresh water, and terrestrial ecosystems are sampled regularly and archived under chemically stable conditions. an initial characterization of the samples provides data regarding the status quo of the respective ecosystems. the aim of the present publication is to give insight into these real-time monitori ... | 2010 | 20058089 |
| conversion of 11-ketoandrostenedione to 11-ketotestosterone by blood cells of six fish species. | blood cells from baltic salmon, salmo salar, three-spined stickleback, gasterosteus aculeatus, eel pout, zoarces viviparus, crucian carp, carassius carassius, african catfish, clarias gariepinus, and reedfish, calamoichthys calabaricus, were incubated with tritiated 11 beta-hydroxyandrostenedione (oha) or 11-ketoandrostenedione (oa). in all fish there was conversion of oa to 11-ketotestosterone (ot), indicating that 17 beta-hydroxysteroid dehydrogenase activity was present in the blood cells. on ... | 1990 | 2295425 |
| assessing the environmental genotoxicity risk in the baltic sea: frequencies of nuclear buds in blood erythrocytes of three native fish species. | nuclear bud (nb) formation was investigated in blood erythrocytes of 1892 flounder platichthys flesus, herring clupea harengus, and eelpout zoarces viviparus specimens that were collected at 82 sites representing different regions of the baltic sea in 2009-2011. this is the first attempt to evaluate the baseline levels of nb and rank the genotoxicity risk for native fish species. nb levels were compared to the previously published micronuclei (mn) data from the same individual fish specimens in ... | 2015 | 25384369 |
| risk of environmental genotoxicity in the baltic sea over the period of 2009-2011 assessed by micronuclei frequencies in blood erythrocytes of flounder (platichthys flesus), herring (clupea harengus) and eelpout (zoarces viviparus). | environmental genotoxicity was investigated at 82 locations encompassing different regions of the baltic sea. micronuclei (mn) analysis was performed in erythrocytes of 1892 specimens of flounder platichthys flesus, herring clupea harengus and eelpout zoarces viviparus, three of the most common native fish species of the baltic sea collected in 2009-2011. mn background levels in fish were determined using data obtained in 2001-2011 from 107 baltic sites. extremely high genotoxicity risk zones we ... | 2012 | 22349255 |
| permanent genetic resources added to molecular ecology resources database 1 october 2011-30 november 2011. | this article documents the addition of 139 microsatellite marker loci and 90 pairs of single-nucleotide polymorphism sequencing primers to the molecular ecology resources database. loci were developed for the following species: aglaoctenus lagotis, costus pulverulentus, costus scaber, culex pipiens, dascyllus marginatus, lupinus nanus benth, phloeomyzus passerini, podarcis muralis, rhododendron rubropilosum hayata var. taiwanalpinum and zoarces viviparus. these loci were cross-tested on the foll ... | 2012 | 22296658 |
| in vivo mr spectroscopy and mr imaging on non-anaesthetized marine fish: techniques and first results. | in vivo magnetic resonance imaging (mri) and spectroscopy (mrs) experiments were carried out in non-anaesthetized marine fish with a maximum body length of 40 cm using a 4.7 t horizontal magnet. in flow-through animal chambers long-term investigations could be carried out for several days in a sessile benthic zoarcid species, the eelpout zoarces viviparus, as well as in a pelagic gadid species, the cod gadus morhua. sea water adapted probes and optimised excitation and refocusing pulse shapes re ... | 2002 | 12034337 |
| evolutionary force in confamiliar marine vertebrates of different temperature realms: adaptive trends in zoarcid fish transcriptomes. | studies of temperature-induced adaptation on the basis of genomic sequence data were mainly done in extremophiles. although the general hypothesis of an increased molecular flexibility in the cold is widely accepted, the results of thermal adaptation are still difficult to detect at proteomic down to the genomic sequence level. approaches towards a more detailed picture emerge with the advent of new sequencing technologies. only small changes in primary protein structure have been shown to modif ... | 2012 | 23051706 |
| characterization of the zoarces viviparus liver transcriptome using massively parallel pyrosequencing. | the teleost zoarces viviparus (eelpout) lives along the coasts of northern europe and has long been an established model organism for marine ecology and environmental monitoring. the scarce information about this species genome has however restrained the use of efficient molecular-level assays, such as gene expression microarrays. | 2009 | 19646242 |
| purification, crystal structure determination and functional characterization of type iii antifreeze proteins from the european eelpout zoarces viviparus. | antifreeze proteins (afps) are essential components of many organisms adaptation to cold temperatures. fish type iii afps are divided into two groups, sp isoforms being much less active than qae1 isoforms. two type iii afps from zoarces viviparus, a qae1 (zvafp13) and an sp (zvafp6) isoform, are here characterized and their crystal structures determined. we conclude that the higher activity of the qae1 isoforms cannot be attributed to single residues, but rather a combination of structural effec ... | 2014 | 25025819 |