| thermothrix thioparus gen. et sp. nov. a facultatively anaerobic facultative chemolithotroph living at neutral ph and high temperature. | thermothrix thioparus gen. et ep. nov. occurs naturally in a new mexico hot spring at a temperature of 74 degrees c, a ph of 7.0, and a hs- concentration of 1 mg/litre. the organism is gram-negative, non-motile, 0.5-1.0 x 3-20 mum, and forms cell chains up to 1 cm in length. the resulting filaments do not possess a sheath. sulfur is deposited extracellularly. the organism was isolated using an autotrophic medium with hs- as the energy source and no3- as the terminal electron acceptor. anaerobica ... | 1976 | 10063 |
| apurinic and apyrimidinic dna endonuclease of extremely thermophilic thermothrix thiopara. | an endonuclease specific for apurinic, apyrimidinic (ap) sites in dna was purified nearly to homogeneity from the extremely thermophilic bacterium thermothrix thiopara. the enzyme has a molecular weight of approximately 26,000. it cleaves neither native nor uv- or gamma-irradiated dnas and has no contaminating exonuclease or uracil-dna glycosylase activities. the enzyme has no cofactor requirement and is not inhibited by edta or n'-ethylmaleimide. it shows maximal activity at 70 degrees c and a ... | 1985 | 2414271 |
| uracil-dna glycosylase of thermophilic thermothrix thiopara. | an activity which released free uracil from dump-containing dna was purified approximately 1,700-fold from extracts of thermothrix thiopara, the first such activity to be isolated from extremely thermophilic bacteria. the enzyme appeared homogeneous, according to the results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis. it had a native molecular weight of 26,000 and existed as a monomer protein in water solution. the enzyme had an optimal activity at 70 degrees c, between ph 7.5 ... | 1985 | 4044527 |
| thermothrix azorensis sp. nov., an obligately chemolithoautotrophic, sulfur-oxidizing, thermophilic bacterium. | a new aerobic, obligately chemolithoautotrophic, thermophilic, sulfur-oxidizing bacterium, thermothrix azorensis, was isolated from a hot spring on sao miguel island in the azores. the cells of this organism are gram negative, nonsporulating, and rod shaped. filament formation appears to occur as a response to nonoptimal growth conditions. growth occurs at 63 to 86 degrees c, and the optimum temperature is 76 to 78 degrees c. the optimum ph range for growth is 7.0 to 7.5. the g+c content of the ... | 1996 | 8934901 |
| desulfurella kamchatkensis sp. nov. and desulfurella propionica sp. nov., new sulfur-respiring thermophilic bacteria from kamchatka thermal environments. | two strains of moderately thermophilic bacteria, which reduce elemental sulfur to hydrogen sulfide, were isolated from volcanic sources in kamchatka. strain k-119t was obtained from a thermophilic microbial community associated with thermothrix thiopara, and strain u-8t was isolated from a cyanobacterial mat inhabiting a sulfide-rich hot spring. cells of both strains are short gram-negative rods, motile with one polar flagellum (strain k-119t) or non-motile (strain u-8t). both strains are obliga ... | 1998 | 9731287 |
| superoxide dismutase biosynthesis by two thermophilic bacteria. | some high-molecular weight antioxidant defense system components of two thermophilic bacteria isolated from spa waters of serbia (yugoslavia) and identified as bacillus stearothermophilus and thermothrix sp. were studied. in addition to superoxide dismutase (sod; ec 1.15.1.1), qualitative analyses demonstrated the presence of catalase (ec 1.11.1.6), peroxidases and oxidases in both bacterial strains. cell-free extracts were subjected to nondenaturing polyacrylamide gel electrophoresis (page) and ... | 2000 | 11118588 |
| thermothrix thiopara: growth and metabolism of a newly isolated thermophile capable of oxidizing sulfur and sulfur compounds. | thermothrix thiopara is isolated from hot sulfur springs. it occurs in situ at a temperature of 72 degrees c, a ph of 7.0, and an hs concentration of 17.4 mumol/liter (0.8 ppm). the organism was capable of autotrophic growth. sulfite, sulfur, and polythionates were formed and subsequently degraded to sulfate during growth with thiosulfate as the sole energy source. thiosulfate was oxidized by the polythionate pathway, and the stoichiometry of growth on thiosulfate was determined. the organism wa ... | 1980 | 16345601 |
| growth kinetics and yield coefficients of the extreme thermophile thermothrix thiopara in continuous culture. | thermothrix thiopara did not appear to be stressed at high temperature (72 degrees c). both the actual and theoretical yields were higher than those of analogous mesophilic sulfur bacteria, and the specific growth rate (mu(max)) was more rapid than that of most autotrophs. the specific growth rate (0.58 h), specific maintenance rate (0.11 h), actual molar growth yield at mu(max) (y(max) = 16 g mol), and theoretical molar growth yield (y(g) = 24 g mol) were all higher for t. thiopara (72 degrees ... | 1983 | 16346163 |
| biological breakdown of denitrifying sulfide removal process in high-rate expanded granular bed reactor. | this work conducted a denitrifying sulfide removal (dsr) test in an expanded granular sludge bed (egsb) reactor at sustainable loadings of 6.09 kg m(-3) day(-1) for sulfide, 3.11 kg m(-3) day(-1) for nitrate-nitrogen, and 3.27 kg m(-1) day(-1) for acetate-carbon with >93% efficiency, which is significantly higher than those reported in literature. strains pseudomonas sp., nitrincola sp., and azoarcus sp. very likely yield heterotrophs. strains thermothrix sp. and sulfurovum sp. are the autotroph ... | 2008 | 18972111 |