| genotypic and phenotypic characterization of the acinetobacter calcoaceticus-acinetobacter baumannii complex with the proposal of acinetobacter pittii sp. nov. (formerly acinetobacter genomic species 3) and acinetobacter nosocomialis sp. nov. (formerly acinetobacter genomic species 13tu). | acinetobacter genomic species (gen. sp.) 3 and gen. sp. 13tu are increasingly recognized as clinically important taxa within the acinetobacter calcoaceticus-acinetobacter baumannii (acb) complex. to define the taxonomic position of these genomic species, we investigated 80 strains representing the known diversity of the acb complex. all strains were characterized by aflp analysis, amplified rdna restriction analysis and nutritional or physiological testing, while selected strains were studied by ... | 2011 | 21320596 |
| acinetobacter infection - an emerging threat to human health. | the genus acinetobacter comprises a complex and heterogeneous group of bacteria, many of which are capable of causing a range of opportunistic, often catheter-related, infections in humans. however, acinetobacter baumannii, as well as its close relatives belonging to genomic species 3 ("acinetobacter pittii") and 13tu ("acinetobacter nosocomialis"), are important nosocomial pathogens, often associated with epidemic outbreaks of infection, that are only rarely found outside of a clinical setting. ... | 2011 | 22006724 |
| Characteristics of carbapenem-resistant Acinetobacter spp. other than Acinetobacter baumannii in South Korea. | Although many studies have been performed on carbapenem-resistant Acinetobacter baumannii, only a few studies have addressed carbapenem resistance in Acinetobacter spp. other than A. baumannii (non-baumannii Acinetobacter). Amongst 287 Acinetobacter spp. isolates from patients with bacteraemia in a South Korean hospital collected between 2003 and 2010, 160 (55.7%) were non-baumannii Acinetobacter spp. Antimicrobial susceptibility testing was performed and the effect of efflux pump inhibitors was ... | 2012 | 21996405 |
| Nosocomial bloodstream infections due to Acinetobacter baumannii, 'Acinetobacterpittii' and 'Acinetobacternosocomialis' in the United States. | OBJECTIVES: To compare the clinical features and antimicrobial susceptibilities of the clinically most important Acinetobacter species Acinetobacterbaumannii, 'Acinetobacterpittii' (formerly Acinetobacter genomic species 3) and 'Acinetobacternosocomialis' (formerly Acinetobacter genomic species 13TU). METHODS: 295 Acinetobacter isolates collected prospectively from patients with bloodstream infections (BSI) in 52 US hospitals were identified to species level. Clinical and microbiological feature ... | 2011 | 22209744 |
| molecular epidemiology of acinetobacter baumannii bloodstream isolates obtained in the united states from 1995 to 2004 using rep-pcr and multilocus sequence typing. | using a repetitive-sequence-based (rep)-pcr (diversilab), we have molecularly typed acinetobacter nosocomial bloodstream isolates (acinetobacter baumannii [n = 187], acinetobacter pittii [n = 23], and acinetobacter nosocomialis [n = 61]) obtained from patients hospitalized in u.s. hospitals over a 10-year period (1995-2004) during a nationwide surveillance study (surveillance and control of pathogens of epidemiological importance [scope]). patterns of a. baumannii rep-pcr were compared to those ... | 2012 | 22895032 |
| first identification of oxa-72 carbapenemase from acinetobacter pittii in colombia. | oxa-72 has been reported in few countries around the world. we report the first case in colombia in an acinetobacter pittii clinical isolate. the arrival of a new oxa, into a country with high endemic resistance, poses a significant threat, especially because the potential for widespread dissemination is considerable. | 2012 | 22508295 |
| expression of the resistance-nodulation-cell division pump adeijk in acinetobacter baumannii is regulated by aden, a tetr-type regulator. | resistance-nodulation-division efflux system adeijk contributes to intrinsic resistance to various drug classes in acinetobacter baumannii. by whole-genome sequencing, we have identified in clinical isolate bm4587 the aden gene, located 813 kbp upstream from adeijk, which encodes a tetr transcriptional regulator. in one-step mutant bm4666 overexpressing adeijk, the deletion of cytosine 582 (c(582)) in the 3' portion of this gene was responsible for a frameshift mutation resulting in the deletion ... | 2012 | 22371895 |
| draft genome sequences of multidrug-resistant acinetobacter sp. strains from colombian hospitals. | the draft genome sequences of the strains acinetobacter baumannii 107m, acinetobacter nosocomialis 28f, and acinetobacter pittii 42f, isolated from colombian hospitals, are reported here. these isolates are causative of nosocomial infections and are classified as multidrug resistant, as they showed resistance to four different antibiotic groups. | 2013 | 24285656 |
| pathogenic acinetobacter: from the cell surface to infinity and beyond. | the genus acinetobacter encompasses multiple nosocomial opportunistic pathogens that are of increasing worldwide relevance because of their ability to survive exposure to various antimicrobial and sterilization agents. among these, acinetobacter baumannii, acinetobacter nosocomialis, and acinetobacter pittii are the most frequently isolated in hospitals around the world. despite the growing incidence of multidrug-resistant acinetobacter spp., little is known about the factors that contribute to ... | 2016 | 26712938 |
| a case of imp-4-, oxa-421-, oxa-96-, and carb-2-producing acinetobacter pittii sequence type 119 in australia. | an imp-4-producing acinetobacter pittii strain coproducing oxacillinases was isolated from a leg wound of a 67-year-old female patient. identification to the species level by rpob and gyrb sequencing and multiplex-pcr-based analysis revealed that the isolate was a. pittii. whole-genome sequencing of this a. pittii isolate determined the presence of blaoxa-96, blacarb-2, and a novel blaoxa-421 gene. the position of this novel blaoxa-421 gene was similar to that of blaoxa-51 in a. baumannii, downs ... | 2014 | 25428154 |
| characterization of plasmids in extensively drug-resistant acinetobacter strains isolated in india and pakistan. | the blandm-1 gene is associated with extensive drug resistance in gram-negative bacteria. this probably spread to enterobacteriaceae from acinetobacter spp., and we characterized plasmids associated with blandm-1 in acinetobacter spp. to gain insight into their role in this dissemination. four clinical ndm-1-producing acinetobacter species strains from india and pakistan were investigated. a plasmid harboring blandm-1, pndm-40-1, was characterized by whole-genome sequencing of acinetobacter bere ... | 2015 | 25421466 |
| lettuce and fruits as a source of multidrug resistant acinetobacter spp. | the role of ready-to-eat products as a reservoir of pathogenic species of acinetobacter remains unclear. the objective of the present study was to evaluate the presence of acinetobacter species in lettuces and fruits marketed in portugal, and their susceptibility to antimicrobials. acinetobacter spp. were isolated from 77.9% of the samples and these microorganisms were also found as endophytes (i.e. present within the plant tissue) in 12 of 20 samples of lettuces analysed. among 253 isolates tha ... | 2017 | 28213015 |
| a new subclass of intrinsic aminoglycoside nucleotidyltransferases, ant(3")-ii, is horizontally transferred among acinetobacter spp. by homologous recombination. | the emergence and spread of antibiotic resistance among acinetobacter spp. have been investigated extensively. most studies focused on the multiple antibiotic resistance genes located on plasmids or genomic resistance islands. on the other hand, the mechanisms controlling intrinsic resistance are still not well understood. in this study, we identified the novel subclass of aminoglycoside nucleotidyltransferase ant(3")-ii in acinetobacter spp., which comprised numerous variants distributed among ... | 2017 | 28152054 |
| prevalence and antimicrobial susceptibility of acinetobacter spp. isolated from meat. | the prevalence and antibiotic resistance of acinetobacter spp. from fifty samples of meat (chicken, turkey, beef and pork) were evaluated. acinetobacter spp. was recovered from all samples and the clonal relatedness of 223 isolates identified to belong to the genus acinetobacter was established by pfge. a high genetic diversity was observed and 166 isolates from different samples, 141 representing different pfge profiles, were further identified to the species level by rpob gene sequencing. thir ... | 2017 | 27992816 |
| draft genome sequence of the environmentally isolated acinetobacter pittii strain ipk_tsa6.1. | acinetobacter pittii is an opportunistic pathogen frequently isolated from acinetobacter infections other than those from acinetobacter baumannii multidrug resistance in a. pittii, including resistance to carbapenems, has been increasingly reported worldwide. here, we report the 4.14-mbp draft genome sequence of a. pittii ipk_tsa6.1 that was isolated from a nonhospital setting. | 2016 | 27688336 |
| clonal transmission of gram-negative bacteria with carbapenemases ndm-1, vim-1, and oxa-23/72 in a bulgarian hospital. | we characterized 72 isolates with reduced susceptibility to carbapenems (50 acinetobacter spp., 13 proteus mirabilis, five escherichia coli, one morganella morganii, one enterobacter cloacae, one providencia rettgeri, and one pseudomonas aeruginosa) from a hospital in sofia, bulgaria. different β-lactamase genes were identified by polymerase chain reaction and sequencing. bacterial strain typing was performed by enzymatic macrorestriction and pulsed-field gel electrophoresis (pfge) typing as wel ... | 2017 | 27459019 |
| acinetobacter dijkshoorniae sp. nov., a member of the acinetobacter calcoaceticus-acinetobacter baumannii complex mainly recovered from clinical samples in different countries. | the recent advances in bacterial species identification methods have led to the rapid taxonomic diversification of the genus acinetobacter. in the present study, phenotypic and molecular methods have been used to determine the taxonomic position of a group of 12 genotypically distinct strains belonging to the acinetobacter calcoaceticus-acinetobacter baumannii (acb) complex, initially described by gerner-smidt and tjernberg in 1993, that are closely related to acinetobacter pittii. strains chara ... | 2016 | 27432448 |
| development and evaluation of species-specific pcr for detection of nine acinetobacter species. | molecular methods have the potential to improve the speed and accuracy of acinetobacter species identification in clinical settings. the goal of this study is to develop species-specific pcr assays based on differences in the rna polymerase beta-subunit gene (rpob) to detect nine commonly isolated acinetobacter species including acinetobacter baumannii, acinetobacter calcoaceticus, acinetobacter pittii, acinetobacter nosocomialis, acinetobacter lwoffii, acinetobacter ursingii, acinetobacter bere ... | 2016 | 27312551 |
| acinetobacter baumannii and a. pittii clinical isolates lack adherence and cytotoxicity to lung epithelial cells in vitro. | the molecular and genetic basis of acinetobacter baumannii and acinetobacter pittii virulence remains poorly understood, and there is still lack of knowledge in host cell response to these bacteria. in this study, we have used eleven clinical acinetobacter strains (a. baumannii n = 5; a. pittii n = 6) to unravel bacterial adherence, invasion and cytotoxicity to human lung epithelial cells. our results showed that adherence to epithelial cells by acinetobacter strains is scarce and cellular invas ... | 2016 | 27235198 |
| molecular investigation of carbapenem-resistant acinetobacter spp. from hospitals in north rhine-westphalia, germany. | emergence of carbapenem-resistant acinetobacter spp., especially acinetobacter baumannii, in hospitals has been increasingly detected worldwide. in the present study, we analyzed carbapenem-resistant isolates (70 a. baumannii and one acinetobacter pittii) collected in a period of 4 years (february 2008 to january 2012) in one diagnostic laboratory in germany. all isolates were carbapenemase positive with oxa-23 as by far the most common enzyme (n = 66, 93%). carbapenemases oxa-24-like and oxa-58 ... | 2017 | 27093111 |
| blood stream infections caused by acinetobacter baumannii group in japan - epidemiological and clinical investigation. | acinetobacter calcoaceticus-acinetobacter baumannii complex, especially a. baumannii, acinetobacter pittii and acinetobacter nosocomialis, constitutes an important group of nosocomial pathogens; however, epidemiological or clinical characteristics and prognosis is limited in japan. from 2009 to 2013, 47 blood stream infection cases resulting from a. baumannii group were reviewed at the national defense medical college, an 800-bed tertiary hospital. to determine the genospecies, further comparati ... | 2016 | 26993173 |
| species identification within acinetobacter calcoaceticus-baumannii complex using maldi-tof ms. | acinetobacter baumannii, one of the more clinically relevant species in the acinetobacter genus is well known to be multi-drug resistant and associated with bacteremia, urinary tract infection, pneumonia, wound infection and meningitis. however, it cannot be differentiated from closely related species such as acinetobacter calcoaceticus, acinetobacter pittii and acinetobacter nosocomialis by most phenotypic tests and can only be differentiated by specific, time consuming genotypic tests with ver ... | 2015 | 26381662 |
| in vitro activity of aminoglycosides against clinical isolates of acinetobacter baumannii complex and other nonfermentative gram-negative bacilli causing healthcare-associated bloodstream infections in taiwan. | aminoglycosides possess in vitro activity against aerobic and facultative gram-negative bacilli. however, nationwide surveillance on susceptibility data of acinetobacter baumannii complex and pseudomonas aeruginosa to aminoglycosides was limited, and aminoglycoside resistance has emerged in the past decade. we study the in vitro susceptibility of a. baumannii complex and other nonfermentative gram-negative bacilli (nfgnb) to aminoglycosides. | 2016 | 26364729 |
| the changing epidemiology of acinetobacter spp. producing oxa carbapenemases causing bloodstream infections in brazil: a brasnet report. | we evaluated the epidemiology of acinetobacter spp. recovered from patients diagnosed with bloodstream infections in 9 tertiary hospitals located in all brazilian geographic regions between april and august 2014. although oxa-23-producing acinetobacter baumannii clones were disseminated in most hospitals, it was observed for the first time the spread of oxa-72 among clonally related a. baumannii isolated from distinct hospitals. interestingly, acinetobacter pittii was the most frequent species f ... | 2015 | 26364001 |
| using vitek maldi-tof mass spectrometry to identify species belonging to the acinetobacter calcoaceticus-acinetobacter baumannii complex: a relevant alternative to molecular biology? | acinetobacter baumannii belongs to the acinetobacter calcoaceticus-baumannii complex (acb) containing 2 other pathogenic species: acinetobacter pittii and acinetobacter nosocomialis. identification of these bacteria remains problematic despite the use of matrix-assisted laser ionization time-of-flight mass spectrometry (maldi-tof ms). here, we enriched the saramis™ database of the vitek ms® plus mass spectrometer to improve the identification of species of the acb complex. for each species, we i ... | 2015 | 26198329 |
| a distinct alleles and genetic recombination of pmrcab operon in species of acinetobacter baumannii complex isolates. | to investigate pmrcab sequence divergence in 5 species of acinetobacter baumannii complex, a total of 80 isolates from a korean hospital were explored. we evaluated nucleotide and amino acid polymorphisms of pmrcab operon, and phylogenetic trees were constructed for each gene of prmcab operon. colistin and polymyxin b susceptibility was determined for all isolates, and multilocus sequence typing was also performed for a. baumannii isolates. our results showed that each species of a. baumannii co ... | 2015 | 25934155 |
| comparison between bacteremia caused by acinetobacter pittii and acinetobacter nosocomialis. | patients with acinetobacter pittii and acinetobacter nosocomialis bacteremia have lower mortality rates than those with acinetobacter baumannii bacteremia. however, it is unknown whether these organisms differ in outcomes of bacteremic patients. we conducted this study to answer this question. | 2017 | 25735795 |
| substitutions of ser83leu in gyra and ser80leu in parc associated with quinolone resistance in acinetobacter pittii. | to investigate the prevalence and the mechanism of quinolone-resistant acinetobacter pittii, 634 acinetobacter calcoaceticus-acinetobacter baumannii complex isolates were collected throughout zhejiang province. identification of isolates was conducted by matrix-assisted laser desorption ionization/time of flight mass spectrometry (maldi-tof ms), blaoxa-51-like gene, and partial rna polymerase β-subunit (rpob) amplification. twenty-seven isolates of a. pittii were identified. among the 634 isolat ... | 2015 | 25514581 |
| influence of acb complex genospecies on clinical outcomes in a u.s. hospital with high rates of multidrug resistance. | bacteria within the acinetobacter calcoaceticus-acinetobacter baumannii (acb) complex commonly cause nosocomial infection and are often multidrug resistant. advances in genospecies typing allow for speciation within the acb complex; however, little is known about the effect of genospecies on patient outcomes. | 2015 | 25246361 |
| early dissemination of oxa-72-producing acinetobacter baumannii strain in colombia: a case report. | nosocomial infections caused by carbapenem-resistant acinetobacter baumannii isolates have reached epidemic levels in past decades. currently this microorganism is responsible for outbreaks of difficult eradication and with high mortality rates worldwide. we herein report a rare case of an oxa-72-producing a. baumannii isolate colonizing a 47-year-old male patient with peritonitis due to abdominal stab wound, four years earlier than the first report of this carbapenemase in acinetobacter pittii ... | 2014 | 25181399 |
| rapid identification of acinetobacter baumannii, acinetobacter nosocomialis and acinetobacter pittii with a multiplex pcr assay. | acinetobacter baumannii, acinetobacter nosocomialis and acinetobacter pittii are clinically relevant members of the acinetobacter calcoaceticus-a. baumannii (acb) complex and important nosocomial pathogens. these three species are genetically closely related and phenotypically similar; however, they differ in their epidemiology, antibiotic resistance and pathogenicity. in this study, we investigated the use of a multiplex pcr-based assay designed to detect internal fragments of the 16s-23s rrna ... | 2014 | 24965800 |
| maldi-tof ms and chemometric based identification of the acinetobacter calcoaceticus-acinetobacter baumannii complex species. | maldi-tof ms is becoming the technique of choice for rapid bacterial identification at species level in routine diagnostics. however, some drawbacks concerning the identification of closely related species such as those belonging to the acinetobacter calcoaceticus-acinetobacter baumannii (acb) complex lead to high rates of misidentifications. in this work we successfully developed an approach that combines maldi-tof ms and chemometric tools to discriminate the six acb complex species (a. baumann ... | 2014 | 24877727 |
| comparative in vitro activity of sitafloxacin against bacteremic isolates of carbapenem resistant acinetobacter baumannii complex. | the emergence of carbapenem-resistant acinetobacter baumannii (crab) complex has posed a great challenge to clinicians worldwide. sitafloxacin has been shown to have in vitro activity against pathogens resistant to other fluoroquinolones. however, data comparing the anti-crab activity of sitafloxacin with that of other antimicrobial agents are limited. | 2015 | 24662017 |
| worldwide dissemination of acquired carbapenem-hydrolysing class d β-lactamases in acinetobacter spp. other than acinetobacter baumannii. | the aim of this study was to identify acquired oxa-type carbapenemases in acinetobacter spp. other than acinetobacter baumannii. from a total of 453 carbapenem-susceptible and -resistant acinetobacter isolates collected worldwide, 23 were positive for blaoxa genes by multiplex pcr. these isolates were identified as acinetobacter pittii (n=18), acinetobacter nosocomialis (n=2), acinetobacter junii (n=1) and acinetobacter genomic species 14tu/13bj (n=2). the blaoxa genes and associated insertion s ... | 2014 | 24612983 |
| characterization of blaoxa-143 variants in acinetobacter baumannii and acinetobacter pittii. | the acquired carbapenem-hydrolyzing oxacillinase (oxa) oxa-143 has thus far been detected only in acinetobacter baumannii isolates from brazil. the aim of this study was to characterize three oxa-143 variants: oxa-231 and oxa-253 from carbapenem-resistant a. baumannii isolates and oxa-255 in a carbapenem-susceptible acinetobacter pittii isolate originating from brazil, honduras, and the united states, respectively. the 5' rapid amplification of cdna ends (race) technique identified the same tran ... | 2014 | 24566181 |
| characterization of a novel plasmid type and various genetic contexts of bla oxa-58 in acinetobacter spp. from multiple cities in china. | several studies have described the epidemiological distribution of blaoxa-58-harboring acinetobacter baumannii in china. however, there is limited data concerning the replicon types of blaoxa-58-carrying plasmids and the genetic context surrounding blaoxa-58 in acinetobacter spp. in china. | 2014 | 24400107 |
| prevalence of carbapenem-hydrolyzing class d β-lactamase genes in acinetobacter spp. isolates in china. | in order to assess the prevalence of carbapenem-hydrolyzing class d β-lactamase genes in acinetobacter spp. isolates in china, we conducted a polymerase chain reaction (pcr)-based surveillance of oxa-type β-lactamase gene clusters for a total of 2,880 acinetobacter spp. isolates collected from 23 chinese provinces. all isolates were tested for susceptibility to 12 antimicrobial agents and showed high rates of resistance to all these agents except minocycline. we also found that the vast majority ... | 2014 | 24374815 |
| identification of 50 class d β-lactamases and 65 acinetobacter-derived cephalosporinases in acinetobacter spp. | whole-genome sequencing of a collection of 103 acinetobacter strains belonging to 22 validly named species and another 16 putative species allowed detection of genes for 50 new class d β-lactamases and 65 new acinetobacter-derived cephalosporinases (adc). all oxacillinases (oxa) contained the three typical motifs of class d β-lactamases, stfk, (f/y)gn, and k(s/t)g. the phylogenetic tree drawn from the oxa sequences led to an increase in the number of oxa groups from 7 to 18. the topologies of th ... | 2014 | 24277043 |
| evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for species identification of acinetobacter strains isolated from blood cultures. | the clinical relevance of acinetobacter species, other than a. baumannii, as human pathogens has not been sufficiently assessed owing to the insufficiency of simple phenotypic clinical diagnostic laboratory tests. infections caused by these organisms have different impacts on clinical outcome and require different treatment and management approaches. it is therefore important to correctly identify acinetobacter species. matrix-assisted laser desorption ionization-time of flight mass spectrometry ... | 2014 | 24125498 |
| molecular genotyping of acinetobacter spp. isolated in arizona, usa, using multilocus pcr and mass spectrometry. | acinetobacter spp. are a diverse group of gram-negative bacteria frequently implicated in nosocomial infections. genotypic methods have been instrumental in studying acinetobacter, but few offer high resolution, rapid turnaround time, technical ease and high inter-laboratory reproducibility, which has hampered understanding of disease incidence, transmission patterns and diversity within this genus. here, we further evaluated multilocus pcr electrospray ionization/mass spectrometry (pcr/esi-ms), ... | 2013 | 23741021 |
| comparative analysis of fecal microflora of healthy full-term indian infants born with different methods of delivery (vaginal vs cesarean): acinetobacter sp. prevalence in vaginally born infants. | in this study fecal microflora of human infants born through vaginal delivery (vb) and through cesarean section (cb) were investigated using culture-independent 16s rdna cloning and sequencing approach. the results obtained clearly revealed that fecal microbiota of vb infants distinctly differ from those in their counterpart cb infants. the intestinal microbiota of infants delivered by cesarean section appears to be more diverse, in terms of bacteria species, than the microbiota of vaginally del ... | 2012 | 23151789 |
| therapeutic options for acinetobacter baumannii infections: an update. | although the clinical importance of acinetobacter pittii and acinetobacter nosocomialis has increased in the nosocomial setting in the last decade, infections caused by acinetobacter baumannii still have the highest clinical relevance. the most important features of this latter species are the ability to persist in the hospital environment and the multi-drug, extended-drug or pandrug resistance they may present which compromises the treatment of infections caused by this microorganism. | 2012 | 23035697 |
| molecular epidemiology of acinetobacter baumannii and acinetobacter nosocomialis in germany over a 5-year period (2005-2009). | to investigate the species distribution within the acinetobacter calcoaceticus-acinetobacter baumannii complex and the molecular epidemiology of a. baumannii and acinetobacter nosocomialis, 376 acinetobacter isolates were collected prospectively from hospitalized patients at 15 medical centres in germany during three surveillance studies conducted over a 5-year period. species identification was performed by molecular methods. imipenem minimum inhibitory concentrations (mic) were determined by b ... | 2013 | 23034071 |
| molecular characterization of carbapenem-non-susceptible acinetobacter spp. in japan: predominance of multidrug-resistant acinetobacter baumannii clonal complex 92 and imp-type metallo-β-lactamase-producing non-baumannii acinetobacter species. | we conducted an epidemiological study concerning carbapenem-non-susceptible clinical isolates of acinetobacter spp. in japan by molecular procedures including carbapenemase gene identification and amplified ribosomal dna restriction analysis. among 598 clinically isolated acinetobacter spp. in 2007, 27 (4.5%) were non-susceptible to carbapenems. most carbapenem-non-susceptible acinetobacter baumannii (13/14) belonged to clonal complex (cc) 92, harbored bla (oxa-51-like) genes, including novel bl ... | 2012 | 22327516 |
| regional dissemination of acinetobacter species harbouring metallo-β-lactamase genes in japan. | metallo-β-lactamase (mbl) producers have been reported among the various acinetobacter species worldwide. in this study, the epidemiology and molecular characteristics of carbapenemase-encoding genes and mobile elements were studied to analyse the regional dissemination of mbl genes in acinetobacter species. from january 2001 to december 2006, 48 acinetobacter isolates harbouring mbl genes identified from five hospitals in kyoto and shiga prefecture, japan were collected and analysed. the partia ... | 2013 | 23009350 |
| acinetobacter pittii and acinetobacter nosocomialis among clinical isolates of the acinetobacter calcoaceticus-baumannii complex in sichuan, china. | among 82 clinical isolates of the acinetobacter calcoaceticus-baumannii complex recovered in 13 hospitals of sichuan, china, in 2011, 13 were acinetobacter pittii and 2 were acinetobacter nosocomialis. multilocus sequence typing revealed a novel sequence type (st) of a. nosocomialis and 7 novel sts of a. pittii. most isolates were hospital-acquired and colonized in the respiratory tract, while 6 cases with pneumonia due to a. pittii were identified. this study provided a snapshot of the local in ... | 2013 | 23639796 |
| effective transfer of a 47 kb ndm-1-positive plasmid among acinetobacter species. | to investigate the link between two ndm-1-positive acinetobacter isolates from the same hospital, the plasmid profiles of the isolates were examined. these two isolates were found from a surveillance programme within 3 months from two patients without obvious physical contact or hospitalization time overlap. | 2015 | 26180132 |
| dissemination and characterization of ndm-1-producing acinetobacter pittii in an intensive care unit in china. | the sporadic emergence of new delhi metallo-β-lactamase-1 (ndm-1)-producing acinetobacter spp. has been reported in china; however, ndm-1-positive bacteria epidemics are rarely reported in intensive care units (icus) in china, or even in the world. during 15 months' surveillance acinetobacter spp. isolated from patients, heathcare workers and surfaces of a chinese icu were screened for the bla(ndm-1) gene. a total of 27 of 3114 acinetobacter spp. strains were ndm-1 positive and identified as a. ... | 2012 | 23036089 |
| polypyrrole-grafted coconut shell biological carbon as a potential adsorbent for methyl tert-butyl ether removal: characterization and adsorption capability. | methyl tert-butyl ether (mtbe) has been used as a common gasoline additive worldwide since the late twentieth century, and it has become the most frequently detected groundwater pollutant in many countries. this study aimed to synthesize a novel microbial carrier to improve its adsorptive capacity for mtbe and biofilm formation, compared to the traditional granular activated carbon (gac). a polypyrrole (ppy)-modified gac composite (ppy/gac) was synthesized, and characterized by fourier transform ... | 2017 | 28125030 |
| description of the metallo-β-lactamase gim-1 in acinetobacter pittii. | to characterize the mechanisms involved in the reduced carbapenem susceptibility of five acinetobacter pittii strains isolated from different regions of germany. | 2014 | 23956376 |
| clinical outcomes of hospital-acquired infection with acinetobacter nosocomialis and acinetobacter pittii. | the role of acinetobacter nosocomialis and acinetobacter pittii, which belong to the a. calcoaceticus-a. baumannii complex, in hospital-acquired infections is increasingly recognized. here we describe a retrospective cohort study of hospital-acquired a. calcoaceticus-a. baumannii complex infections at a university hospital in thailand. a total of 222 unique cases were identified between january 2010 and december 2011. the genomospecies of the a. calcoaceticus-a. baumannii complex isolates were c ... | 2014 | 24820079 |
| acinetobacter lactucae sp. nov., isolated from iceberg lettuce (asteraceae: lactuca sativa). | strain nrrl b-41902t and three closely related strains were isolated from iceberg lettuce. the strain was found to consist of strictly aerobic, gram-stain-negative rods that formed cocci in late stationary phase. 16s rrna gene sequence analysis showed that strain nrrl b-41902t was most closely related to species within the genera acinetobacter, and that a grouping of it and the three other closely related strains was most closely related to the type strain of acinetobacter pittii, which was also ... | 2016 | 27339105 |
| molecular characterisation of carbapenemases in urban pigeon droppings in france and algeria. | the main objective of this study was to detect the presence of carbapenemase-encoding genes in stool samples of urban pigeons. | 2017 | 28487209 |
| sensitivity and specificity of matrix-associated laser desorption/ionization - time of flight mass spectrometry (maldi-tof ms) in discrimination at species level for acinetobacter bacteremia. | we aimed to establish the role of maldi-tof ms on species discrimination of phenotypically indistinguishable a. baumannii, a. pittii and a. nosocomialis. compared to multiplex pcr, the gold standard, maldi-tof ms yielded a high sensitivity for a. baumannii (97.9%) and specificity for a. pittii (98.9%) and a. nosocomialis (100%). | 2017 | 28669800 |
| development and evaluation of a core genome multilocus typing scheme for whole-genome sequence-based typing of acinetobacter baumannii. | we have employed whole genome sequencing to define and evaluate a core genome multilocus sequence typing (cgmlst) scheme for acinetobacter baumannii. to define a core genome we downloaded a total of 1,573 putative a. baumannii genomes from ncbi as well as representative isolates belonging to the eight previously described international a. baumannii clonal lineages. the core genome was then employed against a total of fifty-three carbapenem-resistant a. baumannii isolates that were previously typ ... | 2017 | 28594944 |
| first report of cavitary pneumonia due to community-acquired acinetobacter pittii, study of virulence and overview of pathogenesis and treatment. | acinetobacter pittii is a nosocomial pathogen rarely involved in community-acquired infections. we report for the first time that a. pittii can be responsible for cavitary community-acquired pneumonia and study its virulence, and discuss its pathogenesis and treatment options. | 2017 | 28683724 |
| acinetobacter dijkshoorniae is a later heterotypic synonym of acinetobacter lactucae. | acinetobacter lactucae and acinetobacter dijkshoorniae were recently described as novel species, and both were reported to be closely related to acinetobacter pittii. because they were reviewed and published almost concurrently, their descriptions did not include a specific comparison between these two novel species. genomic data were provided in both initial descriptions, which simplifies the comparisons. genome comparisons based on in silico dna-dna hybridizations, average nucleotide identity ... | 2018 | 29106350 |
| seasonal and temperature-associated increase in community-onset acinetobacter baumannii complex colonization or infection. | identifying the trends in community-onset acinetobacter baumannii complex isolation and diversity according to temperature could help provide insight into the behavior of the a. baumannii complex. we performed a retrospective analysis of a. baumannii complex (acinetobacter baumannii, acinetobacter nosocomialis, acinetobacter pittii, and acinetobacter calcoaceticus) isolates obtained from patients at a korean community hospital from 2006 to 2015 with reference to seasonal temperatures. the incide ... | 2018 | 29401563 |
| defining the interaction of the protease cpaa with its type ii secretion chaperone cpab and its contribution to virulence in acinetobacter species. | acinetobacter baumannii, acinetobacter nosocomialis, and acinetobacter pittii are a frequent cause of multidrug-resistant, healthcare-associated infections. our previous work demonstrated that a. nosocomialis m2 possesses a functional type ii secretion system (t2ss) that is required for full virulence. further, we identified the metallo-endopeptidase cpaa, which has been shown previously to cleave human factor v and deregulate blood coagulation, as the most abundant type ii secreted effector pro ... | 2017 | 28982978 |
| the sesamum indicum rhizosphere associated bacterium: a source of antifungal compound. | the impact of fungal infections on human health has increased considerably within a past few decades. although drugs with antifungal properties are available, but they are less effective and are associated with side effects. | 2018 | 29412110 |
| analysis of the metabolites of indole degraded by an isolated acinetobacter pittii l1. | indole and its derivatives are typical nitrogen heterocyclic compounds and have been of immense concern since they are known for the risk of their toxic, recalcitrant, and carcinogenic properties for human and ecological environment. in this study, a gram-negative bacterial strain of eliminating indole was isolated from a coking wastewater. the strain was confirmed as acinetobacter pittii l1 based on the physiological and biochemical characterization and 16s ribosomal dna (rdna) gene sequence ho ... | 2017 | 29392129 |
| antimicrobial effect of copper alloys on acinetobacter species isolated from infections and hospital environment. | an increased proportion of gram-negative bacteria have recently been reported among etiologic agents of infection. in poland, acinetobacter baumannii is a big problem for hospitals, especially intensive care units. touch surfaces made from materials with antimicrobial properties, especially copper alloys, are recommended as a supplementary method of increasing biological safety in the hospital environment. | 2018 | 29387344 |
| acinetobacter pittii from companion animals coharboring blaoxa-58, the tet(39) region, and other resistance genes on a single plasmid. | | 2018 | 29109166 |
| draft genome sequence of the hydrocarbon-degrading bacterium acinetobacter pittii strain abc isolated from noonmati refinery, assam, india. | we report here the 3.84-mb draft genome sequence of hydrocarbon-degrading acinetobacter pittii strain abc isolated from oil-contaminated soil in guwahati, india. the genome sequence contains 3,602 coding sequences and a g+c content of 38.83%. this is the first report of the genome sequence of an acinetobacter pittii from an oil-contaminated environment. | 2017 | 29097480 |
| uncommon carbapenemase-encoding plasmids in the clinically emergent acinetobacter pittii. | two carbapenemase-carrying plasmids, pls488 (blaoxa-23) and pls535 (blaoxa-58) from acinetobacter pittii clinical isolates, were characterized in this study, including their ability to be transferred to acinetobacter baumannii. | 2018 | 29069366 |
| glimpse into the genome sequence of a multidrug-resistant acinetobacter pittii st950 clinical isolate carrying the blaoxa-72 and blaoxa-533 genes in china. | the development of carbapenem-resistant acinetobacter species is of serious concern in the hospital settings and naturally occurring oxacillinase genes (blaoxa) have been identified in several acinetobacter species. in this study, we report the genome sequence of a. pittii tcm178 belongs to st950, a multidrug-resistant isolate that harbored the blaoxa-72 and blaoxa-533 genes simultaneous. the genome size was estimated to be 3,789,564 bp with 3,501 predicted coding regions, and g+c content is 37. ... | 2017 | 28954001 |
| xylanase from acinetobacter pittii mask 25 and developed magnetic cross-linked xylanase aggregate produce predominantly xylopentose and xylohexose from agro biomass. | most of the chemical and biochemical processes used for the de-polymerization of structural polymers of lignocellulosic biomass are environment unfriendly and costly. here an efficient process based on xylanase, produced by acinetobacter pittii mask25 (mtcc 25132), hydrolysis of only physically treated rice straw and corn cob has been developed for the production of xylooligosaccharides. bacterial strain isolated from soil was found to produce maximum xylanase at 30°c and ph 7. while the optimum ... | 2017 | 28822953 |
| acinetobacter pittii biofilm formation on inanimate surfaces after long-term desiccation. | the survival of pathogenic micro-organisms in the healthcare environment has a major role in nosocomial infections. among the responsible mechanisms enabling nosocomial pathogens to persist with these stress conditions is their ability to resist desiccation and to form biofilms. | 2018 | 28764931 |
| acinetobacter pittii, an emerging new multi-drug resistant fish pathogen isolated from diseased blunt snout bream (megalobrama amblycephala yih) in china. | despite the reason that genus acinetobacter works as a grave human pathogen, very few numbers of researches have been done so that term it as a pathogen in respect to fish. as per the current study, isolation of three pathogenic bacterial strains was carried out from infected blunt snout bream (megalobrama amblycephala yih), from a farm in yixing city, jiangsu province, china, which displayed symptoms like tail-rot, shedding scales and ascites in addition to gentle ulceration on the entire body ... | 2017 | 28755261 |
| whole-genome sequence of acinetobacter pittii humv-6483 isolated from human urine. | acinetobacter pittii strain humv-6483 was obtained from urine from an adult patient. we report here its complete genome assembly using pacbio single-molecule real-time sequencing, which resulted in a chromosome with 4.07 mb and a circular contig of 112 kb. about 3,953 protein-coding genes are predicted from this assembly. | 2017 | 28729271 |
| first report of the carbapenemase gene blaoxa-499 in acinetobacter pittii. | we identified the carbapenemase gene blaoxa-499, a variant of blaoxa-143, from a clinical isolate of acinetobacter pittii for the first time. oxa-499 shared 93.1% amino acid identity with oxa-143, and the gene was located on the chromosome. by cloning the oxa-499-encoding gene into the pwh1266 vector and transforming it into susceptible acinetobacter spp., we were able to show that oxa-499 confers resistance to carbapenems. | 2017 | 28264850 |
| carbapenem-resistant acinetobacter pittii strain harboring blaoxa-72 from brazil. | in this study, we report the isolation of oxa-72-producing acinetobacter pittii in brazil. a carbapenem-resistant a. pittii strain was recovered from a hospitalized female patient from espírito santo, southeastern brazil. pcr screening and dna sequencing allowed us to identify the presence of blaoxa-72. we observed blaoxa-72 in a ~11kb plasmid and flanked by xerc/xerd-binding sites. | 2017 | 28237176 |
| draft genome sequence of a multidrug-resistant beta-lactamase oxa-357-producing acinetobacter pittii st865 clinical isolate from china. | worldwide increasing emergence of carbapenem-resistant acinetobacter spp. has rendered the limited availability of effective antimicrobial agents and has become a major public health concern. in this study, we report the draft genome sequence of a. pittii tcm156, a multidrug-resistant isolate that harbored the blaoxa-357 gene. the genome sequence was further analyzed by various bioinformatics methods. the genome size was estimated to be 3,807,313bp with 3508 predicted coding regions and g+c cont ... | 2017 | 28034597 |
| fortuitous diagnosis of ndm-1-producing acinetobacter pittii carriage in a patient from france with no recent history of travel. | | 2017 | 27999060 |
| draft genome sequence of acinetobacter pittii st643 shared by cystic fibrosis patients. | acinetobacter pittii has emerged as an important hospital pathogen that is associated with outbreaks and drug resistance. in cystic fibrosis (cf) patients, the detection of acinetobacter spp. is rare; however, we isolated the a. pittii sequence type st643 in several brazilian cf patients treated in the same centre. the current study describes the draft genome of a. pittii st643. | 2016 | 27653362 |
| draft genome sequence of a multidrug-resistant new delhi metallo-β-lactamase ndm-1-producing acinetobacter pittii sequence type 207 isolate from china. | the increasing emergence of carbapenem-resistant acinetobacter spp. worldwide has resulted in the limited availability of effective antimicrobial agents and has become a major public health concern. in this study, the draft genome sequence of acinetobacter pittii tcm292 belonging to sequence type 207 (st207), a multidrug-resistant (mdr) isolate harbouring the new delhi metallo-β-lactamase blandm-1 gene, was determined using an illumina hiseq™ 2000 platform. the genome sequence was analysed by bi ... | 2016 | 27530847 |
| emergence of ndm-1 and oxa-72 producing acinetobacter pittii clinical isolates in lebanon. | acinetobacter spp. have emerged as global opportunistic pathogen causing a wide range of infections. emergence of carbapenem resistance in these organisms is a matter of great concern. we report here the first detection of acinetobacter pittii clinical isolates in lebanon carrying either the bla ndm-1 or the bla oxa-72 gene. | 2016 | 27222717 |
| identification of variable-number tandem-repeat (vntr) sequences in acinetobacter pittii and development of an optimized multiple-locus vntr analysis typing scheme. | to develop a multiple-locus variable-number tandem-repeat (vntr) analysis (mlva) assay for acinetobacter pittii typing. | 2015 | 26777905 |
| emergence of st119 acinetobacter pittii co-harbouring ndm-1 and oxa-58 in malaysia. | | 2016 | 26742728 |
| identification of ndm-1 in a putatively novel acinetobacter species ("nb14") closely related to acinetobacter pittii. | in this study, we describe the molecular characterization of a plasmid-located blandm-1 harbored by an acinetobacter clinical isolate recovered from a patient in turkey that putatively constitutes a novel acinetobacter species, as shown by its distinct ardra (amplified 16s ribosomal dna restriction analysis) profile and molecular sequencing techniques. blandm-1 was carried by a conjugative plasmid widespread among non-baumannii acinetobacter isolates, suggesting its potential for dissemination b ... | 2015 | 26259796 |
| emergence of acinetobacter pittii harboring new delhi metallo-beta-lactamase genes in daejeon, korea. | carbapenemase production has been reported worldwide in gram-negative bacteria, including acinetobacter species. we detected carbapenemase-producing acinetobacter pittii in clinical isolates in daejeon, korea. twenty-one ertapenem-resistant a. pittii isolates screened with a disk diffusion method were characterized by using the epsilon test, four multiplex pcr assays, and a multilocus sequence typing (mlst) scheme. a total of 21 a. pittii isolates harbored the metallo-β-lactamase (mbl) gene bla( ... | 2015 | 26206691 |
| use of whole-genome sequencing for characterisation of a st119 ndm-1-producing acinetobacter pittii from a patient in denmark with no history of recent travel. | | 2015 | 26143592 |
| "roar" of blandm-1 and "silence" of blaoxa-58 co-exist in acinetobacter pittii. | acinetobacter pittii 44551 was recovered from a patient with gout combined with tuberculosis and was found to harbor the carbapenemase genes blandm-1 and blaoxa-58 on two different plasmids pndm-44551 and poxa58-44551, respectively. pndm-44551 displayed high self-transferability across multiple bacterial species, while poxa58-44551 was likely co-transferable with pndm-44551 into a. baumannii receipts. pndm-44551 was a close variant of the previously characterized pndm-bj01, and the blandm-1 gene ... | 2015 | 25755005 |
| emergence of ndm-1-producing acinetobacter pittii in brazil. | | 2015 | 25616530 |
| molecular characterization of ndm-1-producing acinetobacter pittii isolated from turkey in 2006. | | 2014 | 25096072 |
| oxa-207, a novel oxa-24 variant with reduced catalytic efficiency against carbapenems in acinetobacter pittii from spain. | a carbapenem-resistant acinetobacter pittii strain carrying an oxa-24-like enzyme was isolated in northern spain in 2008. sequence analysis confirmed the presence of the novel bla(oxa-207) gene flanked by the site-specific xerc/xerd-like recombination binding sites and showing a unique gly222val substitution compared to oxa-24. cloning and kinetic analysis showed that oxa-207 presents a reduction in the catalytic efficiency against carbapenems and a noticeable increase for oxacillin. | 2014 | 24890588 |
| emergence of genetically unrelated ndm-1-producing acinetobacter pittii strains in paraguay. | | 2014 | 24793901 |
| emergence of oxa-72-producing acinetobacter pittii clinical isolates. | | 2014 | 24355573 |
| could acinetobacter pittii act as an ndm-1 reservoir for enterobacteriaceae? | | 2013 | 23732698 |