| lip-like genes in phanerochaete sordida and ceriporiopsis subvermispora, white rot fungi with no detectable lignin peroxidase activity. | lignin peroxidase-like genes were pcr amplified from phanerochaete sordida and ceriporiopsis subvermispora, fungi lacking lignin peroxidase (lip) activity. amplification products were highly similar to previously described lip genes. using reverse transcription-coupled pcr a lip-like cdna clone was amplified from p. sordida rna. in contrast, no evidence was obtained for transcription of c. subvermispora lip genes. | 1996 | 8779605 |
| [effect of cocultivating fungal species on the degradation of lignocellulose residues]. | the degradation potential of phanerochaete sordida, trametes trogii, coprinus truncorum and paecilomyces sp. upon yard wastes was evaluated. the species had been inoculated individually or in pairs formed by p. sordida and paecilomyces sp., t. trogii and paecilomyces sp., and c. truncorum and paecilomyces sp. the highest level of endoxilanase activity was produced by p. sordida growing alone, during day 21 (1.09 u/g of dry material), but in p. sordida and paecilomyces sp. cultures, the detected ... | 2002 | 11942082 |
| purification and characterization of a novel lignin peroxidase from white-rot fungus phanerochaete sordida yk-624. | we characterized kinetics and substrate oxidation of a novel lignin peroxidase (yk-lip) isolated from white-rot fungus phanerochaete sordida yk-624. yk-lip enzyme was identified and purified to homogeneity by anion-exchange chromatography and gel permeation chromatography. the molecular mass of yk-lip was approximately 50 kda, and the absorption spectrum of yk-lip was almost the same as that of the lip (pc-lip) from phanerochaete chrysosporium. steady-state kinetics of veratryl alcohol oxidation ... | 2003 | 12892894 |
| trapping of 2,7-dichlorodibenzo-p-dioxin in aqueous solution by enzymatic reaction of fungal manganese peroxidase in the presence of polyunsaturated fatty acids. | in the presence of polyunsaturated fatty acids, including cis-4,7,10,13,16,19-docosahexaenoic acid (dha), 2,7-dichlorodibenzo-p-dioxin (dcdd) was treated with manganese peroxidase (mnp) from white rot basidiomycete phanerochaete sordida yk-624. after incubation with mnp, dcdd could not be extracted from the reaction mixture with n-hexane and was trapped in the water layer. dcdd was released by alkalification of the water layer. dcdd was also trapped after treatment with lipoxidase, which produce ... | 2003 | 14570278 |
| lignin peroxidase is involved in the biobleaching of manganese-less oxygen-delignified hardwood kraft pulp by white-rot fungi in the solid-fermentation system. | biobleaching of manganese-less oxygen-delignified hardwood kraft pulp (e-okp) by the white-rot fungi phanerochaete sordida yk-624 and p. chrysosporium was examined in the solid-state fermentation system. p. sordida yk-624 possessed a higher brightening activity than p. chrysosporium, increasing pulp brightness by 13.4 points after seven days of treatment. in these fermentation systems, lignin peroxidase (lip) activity was detected as the principle ligninolytic enzyme, and manganese peroxidase an ... | 2004 | 15063497 |
| decolorization of mixtures of different reactive textile dyes by the white-rot basidiomycete phanerochaete sordida and inhibitory effect of polyvinyl alcohol. | we tried to decolorize mixtures of four reactive textile dyes, including azo and anthraquinone dyes, by a white-rot basidiomycete phanerochaete sordida. p. sordida decolorized dye mixtures (200 mg l-1 each) by 90% within 48 h in nitrogen-limited glucose-ammonium media. decolorization of dye mixtures needed mn2+ and tween 80 in the media. manganese peroxidase (mnp) played a major role in dye decolorization by p. sordida. decolorization of dye mixtures by p. sordida was partially inhibited by poly ... | 2005 | 15698645 |
| characteristics of novel lignin peroxidases produced by white-rot fungus phanerochaete sordida yk-624. | we characterized a lignin peroxidase (yk-lip2) isolated from shaking culture inoculated with the white-rot fungus phanerochaete sordida yk-624. the yk-lip2 enzyme was identified and purified to homogeneity by anion-exchange chromatography and gel permeation chromatography. the molecular weight of yk-lip2 was approximately 45 kda, and its absorption spectrum was almost the same as that of the lip (pc-lip) from p. chrysosporium. steady-state kinetics of veratryl alcohol (va) oxidation by yk-lip2 r ... | 2005 | 15869957 |
| third-generation biosensor for lactose based on newly discovered cellobiose dehydrogenase. | the present paper describes the principle and characteristics of a biosensor for lactose based on a third-generation design involving cellobiose dehydrogenase. as resulted from a previous comparative study (submitted manuscript), the novelty of this lactose biosensor is based on highly efficient direct electron transfer between two newly discovered cellobiose dehydrogenases (cdh), from the white rot fungi trametes villosa and phanerochaete sordida, and a solid spectrographic graphite electrode. ... | 2006 | 16408919 |
| removal of estrogenic activity of 4-tert-octylphenol by ligninolytic enzymes from white rot fungi. | 4-tert-octylphenol (4-t-op) was treated with the white rot fungus phanerochaete sordida yk-624 under ligninolytic condition with low-nitrogen and high-carbon culture medium. 4-t-op completely disappeared after 5 days of treatment and the activities of ligninolytic enzymes, laccase and manganese peroxidase (mnp), were detected during this period, thus suggesting that the disappearance of 4-t-op is related to these extracellular enzymes. therefore, 4-t-op was treated with laccase and mnp prepared ... | 2007 | 17497634 |
| transformation by complementation of a uracil auxotroph of the hyper lignin-degrading basidiomycete phanerochaete sordida yk-624. | phanerochaete sordida yk-624 is a hyper lignin-degrading basidiomycete possessing greater ligninolytic selectivity than either p. chrysosporium or trametes versicolor. to construct a gene transformation system for p. sordida yk-624, uracil auxotrophic mutants were generated using a combination of ultraviolet (uv) radiation and 5-fluoroorotate resistance as a selection scheme. an uracil auxotrophic strain (uv-64) was transformed into a uracil prototroph using the marker plasmid ppsura5 containing ... | 2007 | 17701036 |
| biodegradation of orange g by wood-rot fungi phanerochaete sordida txj-1302a and tyromyces lauteus txj-1302b. | two strains isolated from the organic layers of forests on zijin mountain have indicated a strong capability of decolorization for orange g on the solid plates. they were identified as phanerochaete sordida and tyromyces lauteus according to phenotypic and molecular techniques. through this study, we try to find the suitable condition and cheapest way for decolorization by two strains. the result shows that malt extract and ammonium sulfate are the best n source for p. sordida and t. lauteus, re ... | 2008 | 17826087 |
| fungal inoculum properties: extracellular enzyme expression and pentachlorophenol removal in highly contaminated field soils. | this study was conducted to improve the pentachlorophenol (pcp) bioremediation ability of white-rot fungi in highly contaminated field soils by manipulating bioaugmentation variables. these were the dry weight percentage of fungal inoculum addition (31-175 g kg(-1)), pcp concentration (100-2137 mg kg(-1) pcp), fungal inoculum formulation, and time (1-7 wk). five fungal isolates were used: the new zealand isolates trametes versicolor (l.: fr.) hr131 and trametes sp. hr577; the north american isol ... | 2007 | 17940259 |
| removal of diclofenac and mefenamic acid by the white rot fungus phanerochaete sordida yk-624 and identification of their metabolites after fungal transformation. | the non-steroidal anti-inflammatory drugs diclofenac (dcf) and mefenamic acid (mfa) were treated with the white rot fungus phanerochaete sordida yk-624. dcf completely disappeared and mfa decreased by about 90% after 6 days of treatment. it was also confirmed that the fungus almost completely removed the acute lethal toxicity of dcf and mfa towards the freshwater crustacean thamnocephalus platyurus after 6 days of treatment. mass spectrometric and (1)h nuclear magnetic resonance analyses demonst ... | 2010 | 20127144 |
| degrading ability of oligocyclic aromates by phanerochaete sordida selected via screening of white rot fungi. | seventy-nine white rot strains were screened to determine if they had the potential for use in the degradation of oligocyclic aromates (pahs) by measuring their dye-decoloration rate. fourteen strains that were selected based on their dye-decoloration rate were then evaluated for the ability to tolerate various levels of pahs spiked in agar medium. the ability of white rot fungi to degrade 3- or 4-ring pahs (anthracene, phenanthrene, fluoranthene, pyrene) was determined. two strains of phaneroch ... | 2010 | 20941579 |
| black liquor decolorization by selected white-rot fungi. | five different strains of white-rot fungi have been tested for their ability to decolorize black liquor on plates and on solid-state fermentation using vermiculite as the solid inert support. since the high salt concentration inhibited the growth of all fungi, the black liquor was dialyzed against distilled water prior to use. a preliminary step on plates was carried out to qualitatively determine the capacity of the fungal strains for black liquor decolorization. out of the five fungi studied, ... | 2011 | 21499784 |
| Effective Removal of Endocrine-Disrupting Compounds by Lignin Peroxidase from the White-Rot Fungus Phanerochaete sordida YK-624. | The removal of endocrine-disrupting compounds (EDCs) by lignin peroxidase from white-rot fungus Phanerochaete sordida YK-624 (YK-LiP1) was investigated. Five endocrine disruptors, p-t-octylphenol (OP), bisphenol A (BPA), estrone (E(1)), 17ß-estradiol (E(2)), and ethinylestradiol (EE(2)) were eliminated by YK-LiP1 more effectively than lignin peroxidase from P. chrysosporium (Pc-LiP), and OP and BPA were disappeared almost completely in the reaction mixture containing YK-LiP1 after a 24-h treatme ... | 2011 | 22203416 |
| electrochemical investigation of cellobiose dehydrogenase from new fungal sources on au electrodes. | following previous electrochemical investigations of cellobiose dehydrogenase (cdh), the present investigation reports on the initial screening of the electrochemistry of three new cdhs, two from the white rot basidiomycetes trametes villosa and phanerochaete sordida and one from the soft rot ascomycete myriococcum thermophilum, for their ability to directly exchange electrons with 10 different alkanethiol-modified au electrodes. direct electron transfer (det) between the enzymes and some of the ... | 2005 | 15741070 |
| manganese peroxidases of the white rot fungus phanerochaete sordida. | the ligninolytic enzymes produced by the white rot fungus phanerochaete sordida in liquid culture were studied. only manganese peroxidase (mnp) activity could be detected in the supernatant liquid of the cultures. lignin peroxidase (lip) and laccase activities were not detected under a variety of different culture conditions. the highest mnp activity levels were obtained in nitrogen-limited cultures grown under an oxygen atmosphere. the enzyme was induced by mn(ii). the initial ph of the culture ... | 1994 | 8135519 |
| detection of phanerochaete chrysosporium in soil by pcr and restriction enzyme analysis. | a nonradioactive method to detect phanerochaete chrysosporium grown in a soil matrix was developed. this method involved dna extraction, pcr amplification, and restriction enzyme analysis. amplification of ligninase h8 dna from pure cultures of p. chrysosporium was not as sensitive as amplification of the internal transcribed spacer (its) of the highly repetitive nuclear ribosomal dna. amplified its dna was digested with restriction enzymes for analysis. the restriction enzyme pattern of pcr-amp ... | 1994 | 8074515 |
| removal of estrogenic activity of natural steroidal hormone estrone by ligninolytic enzymes from white rot fungi. | natural steroidal hormone estrone (e1) was treated with the white rot fungus phanerochaete sordida yk-624 under ligninolytic condition with low-nitrogen and high-carbon culture medium. e1 decreased by 98% after 5 d of treatment and the activities of ligninolytic enzymes, manganese peroxidase (mnp) and laccase, were detected during treatment, which suggested that the disappearance of e1 is related to ligninolytic enzymes produced extracellularly by white rot fungus. therefore, e1 was treated with ... | 2006 | 16584756 |
| bleaching of hardwood kraft pulp with manganese peroxidase from phanerochaete sordida yk-624 without addition of mnso(inf4). | in vitro bleaching of an unbleached hardwood kraft pulp was performed with partially purified manganese peroxidase (mnp) from the fungus phanerochaete sordida yk-624 without the addition of mnso(inf4) in the presence of oxalate, malonate, or gluconate as manganese chelator. when the pulp was treated without the addition of mnso(inf4), the pulp brightness increased by about 10 points in the presence of 2 mm oxalate, but the brightness did not significantly increase in the presence of 50 mm malona ... | 1996 | 16535279 |
| biodegradation of simple chemical mixtures in soil. | exogenous microorganisms often are used to enhance bioremediation. this study compared the capabilities of two exogenous microbial cultures and an indigenous population to detoxify a weswood silt loam soil amended with a simple chemical mixture. the first three treatments were unamended soils inoculated with either indigenous microorganisms, pseudomonas aeruginosa, or phanerochaete sordida. three additional treatments consisted of soil amended with benzo[a]pyrene, pentachlorophenol, and 2,4,6-tr ... | 2005 | 16398121 |
| bleaching of hardwood kraft pulp with manganese peroxidase secreted from phanerochaete sordida yk-624. | in vitro bleaching of an unbleached hardwood kraft pulp was performed with manganese peroxidase (mnp) from the fungus phanerochaete sordida yk-624. when the kraft pulp was treated with partially purified mnp in the presence of mnso(4), tween 80, and sodium malonate with continuous addition of h(2)o(2) at 37 degrees c for 24 h, the pulp brightness increased by about 10 points and the kappa number decreased by about 6 points compared with untreated pulp. the pulp brightness was also increased by 4 ... | 1994 | 16349458 |
| in vitro bleaching of hardwood kraft pulp by extracellular enzymes excreted from white rot fungi in a cultivation system using a membrane filter. | to clarify the role of excreted extracellular enzymes during long-term incubation in a pulp biobleaching system with white rot fungi, we developed a cultivation system in which a membrane filter is used; this membrane filter can prevent direct contact between hyphae and kraft pulp, but allows extracellular enzymes to attack the kraft pulp. phanerochaete sordida yk-624 brightened the pulp 21.4 points to 54.0% brightness after a 5-day in vitro treatment; this value was significantly higher than th ... | 1994 | 16349219 |
| sensitivity to and degradation of pentachlorophenol by phanerochaete spp. | this research measured mycelial extension rates of selected strains of phanerochaete chrysorhiza, phanerochaete laevis, phanerochaete sanguinea, phanerochaete filamentosa, phanerochaete sordida, inonotus circinatus, and phanerochaete chrysosporium and the ability of these organisms to tolerate and degrade the wood preservative pentachlorophenol (pcp) in an aqueous medium and in soil. most of the tested species had mycelial extension rates in the range of </=0.5 to 1.5 cm day, but there were larg ... | 1990 | 16348355 |
| decolorization of azo dye by the white-rot basidiomycete phanerochaete sordida and by its manganese peroxidase. | we investigated the decolorization of an azo-reactive dye, reactive red 120, by a white-rot basidiomycete, phanerochaete sordida strain yk-624. in liquid culture of p. sordida in a medium containing 3% malt extract and 200 mg/l of the dye, the dye was 90.6% decolorized after 7 d. manganese peroxidase (mnp) activity was detected during the decolorization process. the dye could be decolorized by purified mnp of p. sordida in the presence of mn(ii) and tween 80. the involvement of lipid peroxidatio ... | 2003 | 16233439 |
| removal of estrogenic activity of endocrine-disrupting genistein by ligninolytic enzymes from white rot fungi. | endocrine-disrupting genistein was treated with the white rot fungus phanerochaete sordida yk-624 under ligninolytic condition with low-nitrogen and high-carbon culture medium. genistein decreased by 93% after 4 days of treatment and the activities of ligninolytic enzymes, manganese peroxidase (mnp) and laccase, were detected during treatment, thus suggesting that the disappearance of genistein is related to ligninolytic enzymes produced extracellularly by white rot fungi. therefore, genistein w ... | 2005 | 15727826 |
| dimerization of bisphenol a by hyper lignin-degrading fungus phanerochaete sordida yk-624 under ligninolytic condition. | bisphenol a (bpa) was treated with hyper lignin-degrading fungus phanerochaete sordida yk-624 under ligninolytic condition. after preculturing p. sordida yk-624 for 4 days, bpa (final concentration, 1 and 0.1 mm) was added to cultures. both 1- and 0.1-mm bpa were effectively decreased within a 24-h treatment and two metabolites were detected. two metabolites (5,5'-bis-[1-(4-hydroxy-phenyl)1-methyl-ethyl]-biphenyl-2,2'-diol and 4-(2-(4-hydroxy-phenyl) propan-2-yl)-2-(4-(2-(4-hydroxyphenyl) propan ... | 2013 | 23334298 |
| improvement of ligninolytic properties in the hyper lignin-degrading fungus phanerochaete sordida yk-624 using a novel gene promoter. | we identified a highly expressed protein (buna2) by two-dimensional gel electrophoresis from the hyper lignin-degrading fungus phanerochaete sordida yk-624 under wood-rotting conditions. partial amino acid sequences of buna2 were determined by lc-ms/ms analysis, and buna2 gene (bee2) and promoter region were pcr-cloned and sequenced. the bee2 promoter was used to drive the expression of the manganese peroxidase gene (mnp4) in p. sordida yk-624. eighteen mnp4-expressing clones were obtained, with ... | 2012 | 22506973 |
| burkholderia sordidicola sp. nov., isolated from the white-rot fungus phanerochaete sordida. | two bacterial strains associated with the white-rot fungus phanerochaete sordida were subjected to taxonomic investigation. the isolates, designated kctc 12081(t) and kctc 12082, were gram-negative, non-motile, non-spore-forming and ovoid to rod-shaped. the strains contained major amounts of hexadecanoic acid, cyclo-heptadecanoic acid and omega-7-cis-octadecenoic acid in their cell envelopes. strain kctc 12081(t) contained ubiquinone-8 as the major isoprenoid quinone and the g+c content of its g ... | 2003 | 13130061 |
| degradation of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans by the white rot fungus phanerochaete sordida yk-624. | a method for the degradation of dioxins by white rot fungi was developed. degradation of a mixture of 10 kinds of tetra- to octachlorodibenzo-p-dioxins (polychlorinated dibenzo-p-dioxins [pcdds]) and tetra- to octachlorodibenzofurans (polychlorinated dibenzofurans [pcdfs]), which were chlorinated at 2-, 3-, 7-, and 8-positions of the molecules, by the white rot fungus phanerochaete sordida yk-624 was studied in a stationary low-nitrogen medium. the percent degradation values of pcdds and pcdfs w ... | 1996 | 8953705 |
| bioremediation of the neonicotinoid insecticide clothianidin by the white-rot fungus phanerochaete sordida. | clothianidin (clo) is a member of the neonicotinoid pesticides, which have been widely used worldwide over the last two decades. however, its toxicity for bees and neurological toxicity for humans are urgent problems. here, the degradation of clo by the white-rot fungus phanerochaete sordida was examined in nitrogen-limited liquid medium. after incubation for 20days at 30°c, 37% of clo was degraded in the cultures. high-resolution esi-ms and nmr analyses of the culture supernatant identified n-( ... | 2017 | 27694022 |
| phanerochaete sordida as a cause of pulmonary nodule in an immunocompromised patient: a case report. | phanerochaete sordida is a species of wood rotting fungus, which can degrade lignin, cellulose and hemicellulose contained in wood and other hard-to-biodegrade organic substances. however, to date, there have been no other reports demonstrating that p. sordida can infect humans. | 2017 | 28183273 |
| direct lactic acid production from beech wood by transgenic white-rot fungus phanerochaete sordida yk-624. | a lactic acid (la)-producing strain of the hyper-lignin-degrading fungus phanerochaete sordida yk-624 with the lactate dehydrogenase-encoding gene from bifidobacterium longum (blldh) was constructed. when the endogenous pyruvate decarboxylase gene-knocked down and blldh-expressing transformant was cultured with beech wood meal, the transformant was able to successively delignify and ferment the substrate. supplementation of calcium carbonate into the culture medium, significantly increased the l ... | 2016 | 27746306 |
| endophytic fungi from vitis labrusca l. ('niagara rosada') and its potential for the biological control of fusarium oxysporum. | we investigated the diversity of endophytic fungi found on grape (vitis labrusca cv. niagara rosada) leaves collected from salesópolis, sp, brazil. the fungi were isolated and characterized by amplified ribosomal dna restriction analysis, followed by sequencing of the its1-5.8s-its2 rdna. in addition, the ability of these endophytic fungi to inhibit the grapevine pathogen fusarium oxysporum f. sp herbemontis was determined in vitro. we also observed that the climatic factors, such as temperature ... | 2012 | 23315803 |
| diversity and antimicrobial activities of the fungal endophyte community associated with the traditional brazilian medicinal plant solanum cernuum vell. (solanaceae). | the diversity and antimicrobial activity of endophytic fungi associated with the brazilian medicinal plant solanum cernuum vell. were studied during summer and winter seasons. a total of 246 fungal isolates were obtained, including 225 filamentous fungi and 21 yeasts. they were identified by morphological, physiological, and molecular methods. fifty-five different taxa represented by the phyla ascomycota (33 taxa), basidiomycota (21 taxa), and zygomycota (one taxon) were identified. the most a ... | 2011 | 22182199 |
| biotransformation of acetamiprid by the white-rot fungus phanerochaete sordida yk-624. | acetamiprid (ace) belongs to the neonicotinoid class of systemic broad-spectrum insecticides, which are the most highly effective and largest-selling insecticides worldwide for crop protection. as neonicotinoid insecticides persist in crops, biotransformation of these insecticides represents a promising approach for improving the safety of foods. here, the elimination of ace from a liquid medium by the white-rot fungus phanerochaete sordida yk-624 was examined. under ligninolytic and non-lignino ... | 2011 | 21713509 |
| cellobiose dehydrogenase aryl diazonium modified single walled carbon nanotubes: enhanced direct electron transfer through a positively charged surface. | one of the challenges in the field of biosensors and biofuel cells is to establish a highly efficient electron transfer rate between the active site of redox enzymes and electrodes to fully access the catalytic potential of the biocatalyst and achieve high current densities. we report on very efficient direct electron transfer (det) between cellobiose dehydrogenase (cdh) from phanerochaete sordida (pscdh) and surface modified single walled carbon nanotubes (swcnt). sonicated swcnts were adsorbed ... | 2011 | 21417322 |
| cloning and transcriptional analysis of the gene encoding 5-aminolevulinic acid synthase of the white-rot fungus phanerochaete sordida yk-624. | in this study, we cloned the gene encoding 5-aminolevulinic acid synthase (alas) from the hyper-lignin-degrading fungus phanerochaete sordida yk-624. the deduced amino acid sequence showed highest identity (93.0%) to alas of p. chrysosporium. expression of the gene encoding alas, which we named aas, corresponded temporally with the expression and activity of manganese peroxidase. | 2011 | 21228472 |
| detoxification of aflatoxin b1 by manganese peroxidase from the white-rot fungus phanerochaete sordida yk-624. | aflatoxin b(1) (afb(1) ) is a potent mycotoxin with mutagenic, carcinogenic, teratogenic, hepatotoxic, and immunosuppressive properties. in order to develop a bioremediation system for afb(1) -contaminated foods by white-rot fungi or ligninolytic enzymes, afb(1) was treated with manganese peroxidase (mnp) from the white-rot fungus phanerochaete sordida yk-624. afb(1) was eliminated by mnp. the maximum elimination (86.0%) of afb(1) was observed after 48 h in a reaction mixture containing 5 nkat o ... | 2010 | 21118293 |
| cloning and homologous expression of novel lignin peroxidase genes in the white-rot fungus phanerochaete sordida yk-624. | two genes, encoding yk-lip1 and yk-lip2, were cloned from the white-rot fungus phanerochaete sordida yk-624, and a homologous expression system for the gene was constructed. two full-length cdnas (ylpa and ylpb) were isolated by degenerate rt-pcr and race-pcr. the results of n-terminal amino acid sequence analysis of native yk-lip1 and yk-lip2 showed that ylpa and ylpb coded for yk-lip2 and yk-lip1 respectively. the promoter of glyceraldehyde-3-phosphate dehydrogenase cloned from p. sordida yk-6 ... | 2009 | 19661691 |
| comparison of direct and mediated electron transfer for cellobiose dehydrogenase from phanerochaete sordida. | direct and mediated electron transfer (det and met) between the enzyme and electrodes were compared for cellobiose dehydrogenase (cdh) from the basidiomycete phanerochaete sordida (pscdh). for det, pscdh was adsorbed at pyrolytic graphite (pg) electrodes while for met the enzyme was covalently linked to a low potential os redox polymer. both types of electrodes were prepared in the presence of single walled carbon nanotubes (swcnts). det requires the oxidation of the heme domain, while met occur ... | 2009 | 19256522 |
| accurate delimitation of phanerochaete chrysosporium and phanerochaete sordida by specific pcr primers and cultural approach. | white rot fungi, phanerochaete chrysosporium and phanerochaete sordida, have been mostly studied in a variety of industrial processes like biopulping and pulp bleaching as well as in bioremediation. whereas p. sordida is widely distributed in the north temperate zone, p. chrysosporium is reported in the restricted area and hundreds of reports have been described from a few strains of p. chrysosporium, which are deposited at various fungal collections in the world. the isolates of two species are ... | 2007 | 18050951 |
| improving xylitol production through recombinant expression of xylose reductase in the white-rot fungus phanerochaete sordida yk-624. | we generated an expression construct consisting of the xylose reductase (xr) gene (xr) from phanerochaete chrysosporium. transformant x7 exhibited increased xylitol production and markedly higher xr activities than the wild-type strain. rt-pcr analysis demonstrated that the increased xr activity was associated with constant expression of the recombinant xr gene. | 2015 | 25547244 |
| improvement of ligninolytic properties by recombinant expression of glyoxal oxidase gene in hyper lignin-degrading fungus phanerochaete sordida yk-624. | glyoxal oxidase (glox) is a source of the extracellular h2o2 required for the oxidation reactions catalyzed by the ligninolytic peroxidases. in the present study, the glox-encoding gene (glx) of phanerochaete chrysosporium was cloned, and bee2 promoter of p. sordida yk-624 was used to drive the expression of glx. the expression plasmid was transformed into a p. sordida yk-624 uracil auxotrophic mutant (strain uv-64), and 16 clones were obtained as glox-introducing transformants. these transforma ... | 2014 | 25117933 |
| heterologous expression of a lectin from pleurocybella porrigens (ppl) in phanerochaete sordida yk-624. | pleurocybella porrigens is a mushroom-forming fungus, which had been consumed as a traditional food in japan. however, in 2004, 55 people got poisoned by eating the mushroom and 17 people among them died of acute encephalopathy. we have already reported the purification, characterization, and cdna cloning of a lectin from the mushroom (ppl) which might have caused the poisoning. here, we report the heterologous expression of recombinant ppl by basidiomycete phanerochaete sordida yk-624. the glyc ... | 2014 | 24631556 |
| metabolism of bisphenol a by hyper lignin-degrading fungus phanerochaete sordida yk-624 under non-ligninolytic condition. | recently, we reported the conversion of bisphenol a (bpa) to 4-(2-(4-hydroxyphenyl)propan-2-yl)benzene-1,2-diol (hydroxy-bpa) by hyper lignin-degrading fungus phanerochaete sordida yk-624 under non-ligninolytic condition. in the present study, the metabolism of hydroxy-bpa by p. sordida yk-624 was demonstrated under non-ligninolytic condition. under these conditions, approximately 66% of hydroxy-bpa was degraded after 7 d of incubation. high-resolution electrospray ionization mass spectra and nu ... | 2014 | 24582362 |
| white rot basidiomycetes isolated from chiloé national park in los lagos region, chile. | wood decomposition is an important component in forest ecosystems but information about the diversity of fungi causing decay is lacking. this is especially true for the temperate rain forests in chile. these investigations show results of a biodiversity study of white-rot fungi in wood obtained from chiloé national park in los lagos region, chile. culturing from white-rotted wood followed by sequencing of the complete internal transcribed spacer region of the ribosomal dna (rdna) or partial larg ... | 2013 | 24068453 |
| hydroxylation of bisphenol a by hyper lignin-degrading fungus phanerochaete sordida yk-624 under non-ligninolytic condition. | bisphenol a (bpa) is one of the representative compounds of the endocrine disrupting compounds group and the highest volume chemicals produced worldwide. as a result, bpa is often detected in many soil and water environments. in this study, we demonstrated the transformation of bpa from liquid cultures inoculated with hyper lignin-degrading fungus phanerochaete sordida yk-624. under non-ligninolytic condition, approximately 80% of bpa was eliminated after 7d of incubation. high-resolution electr ... | 2013 | 23942019 |
| improvement of manganese peroxidase production by the hyper lignin-degrading fungus phanerochaete sordida yk-624 by recombinant expression of the 5-aminolevulinic acid synthase gene. | the manganese peroxidase (mnp) gene (mnp4) promoter of phanerochaete sordida yk-624 was used to drive expression of 5-aminolevulinic acid synthase (als), which is a key heme biosynthesis enzyme. the expression plasmid pmnp4pro-als was transformed into p. sordida yk-624 uracil auxotrophic mutant uv-64, and 14 recombinant als expressing-transformants were generated. average cumulative mnp activities in the transformants were 1.18-fold higher than that of control transformants. in particular, trans ... | 2013 | 23884595 |
| effects of homologous expression of 1,4-benzoquinone reductase and homogentisate 1,2-dioxygenase genes on wood decay in hyper-lignin-degrading fungus phanerochaete sordida yk-624. | we investigated the function of 1,4-benzoquinone reductase (bqr)- and homogentisate 1,2-dioxygenase (hgd)-like genes in wood degradation by phanerochaete sordida yk-624, which exhibits high ligninolytic activity and selectivity. we determined homologous expression in the genomic and cdna sequences of bqr- and hgd-like genes in p. sordida yk-624 (psbqr and pshgd). both genes shared high homology (≥90 % amino acid sequence similarity) with the corresponding genes in phanerochaete chrysosporium. th ... | 2016 | 27363425 |
| improvement of ethanol production by recombinant expression of pyruvate decarboxylase in the white-rot fungus phanerochaete sordida yk-624. | to improve ethanol production by phanerochaete sordida yk-624, the pyruvate decarboxylase (pdc) gene was cloned from and reintroduced into this hyper lignin-degrading fungus; the gene encodes a key enzyme in alcoholic fermentation. we screened 16 transformant p. sordida yk-624 strains that each expressed a second, recombinant pdc gene (pdc) and then identified the transformant strain (designated gp7) with the highest ethanol production. direct ethanol production from hardwood was 1.41 higher wit ... | 2016 | 26766784 |
| carboxylated or aminated polyaniline-multiwalled carbon nanotubes nanohybrids for immobilization of cellobiose dehydrogenase on gold electrodes. | polymer-multiwalled carbon nanotube (mwcnt) nanohybrids, which differ in surface charge have been synthesized to study the bioelectrocatalysis of adsorbed cellobiose dehydrogenase (cdh) from phanerochaete sordida on gold electrodes. to obtain negatively charged nanohybrids, poly(3-amino-4-methoxybenzoic acid-co-aniline) (p(amb-a)) was covalently linked to the surface of mwcnts while modification with p-phenylenediamine (pda) converted the cooh-groups to positively charged amino groups. fourier t ... | 2014 | 25587429 |