| photosynthetic symbionts of aeschynomene spp. form a cluster with bradyrhizobia on the basis of fatty acid and rrna analyses. | the relationship between photosynthetic rhizobia that nodulate 10 aeschynomene species (aeschynomene afraspera, aeschynomene denticulata, aeschynomene evenia, aeschynomene indica, aeschynomene nilotica, aeschynomene pratensis, aeschynomene rudis, aeschynomene scabra, aeschynomene schimperi, and aeschynomene sensitiva) and reference strains of the genera bradyrhizobium, rhizobium, and azorhizobium was investigated by analyzing cellular fatty acid methyl esters (fame) and 16s rrna sequences. the m ... | 1994 | 7520731 |
| the aquatic budding bacterium blastobacter denitrificans is a nitrogen-fixing symbiont of aeschynomene indica. | blastobacter spp. are freshwater bacteria that form rosette structures by cellular attachment to a common base. comparative analyses of ribosomal 16s rrna gene and internally transcribed spacer region sequences indicated that b. denitrificans is a member of the alpha-subdivision of proteobacteria. among the alpha-proteobacteria, b. denitrificans was related to a cluster of genera, including rhodopseudomonas palustris, afipia felis, nitrobacter hamburgensis, and bradyrhizobium spp. although the p ... | 2002 | 11872460 |
| symmetric focal degeneration in the cerebellar and vestibular nuclei in swine caused by ingestion of aeschynomene indica seeds. | a vestibulocerebellar disorder was observed in 2- to 4-month-old swine after consumption of broken rice contaminated with 13% aeschynomene indica seeds. affected animals recovered in 2-14 days after removal of the contaminated food. to reproduce the syndrome, 10 pigs were fed 13% a. indica seeds in commercial pig ration or rice. they showed clinical signs similar to those observed in the spontaneous cases, 1 hour to 6 days after ingestion. three pigs recovered after the withdrawal of the contami ... | 2003 | 12724573 |
| neurotoxicity to pigs and rodents from different fractions of aeschynomene indica seeds. | aeschynomene indica seeds cause a vestibulo-cerebellar syndrome in pigs. this experiment studied the toxicity of different plant chemical fractions in pigs to determine a susceptible laboratory species to search for the plant's toxic principle. hexanic, ethanolic and acetonic extracts of a. indica seeds were administered to 1 pig each. the ethanolic extract killed the experimental pig and 2/4 mice and 0/4 rats. the ethanolic extract was fractionated into ethyl acetate, butanolic. and aqueous rem ... | 2003 | 12882484 |
| focal symmetrical encephalomalacia in swine from ingestion of aeschynomene indica seeds. | a spontaneous outbreak of neurological disease in swine caused by the ingestion of aeschynomene indica seeds and reproduction of the disease in the same specie are reported. in the natural outbreak the morbidity, mortality and lethality rates were respectively 25%-40%, 8.5%-20%, and 25%-66%. clinical signs were variable degrees of incoordination of gait, falls, sternal recumbency with wide base stance of the hind limbs, lateral recumbency and death. for experimental reproduction of the intoxicat ... | 2004 | 15587245 |
| bacteriochlorophyll and photosynthetic reaction centers in rhizobium strain btai 1. | rhizobium strain btai 1, which nodulates both stems and roots of aeschynomene indica l., formed bacteriochlorophyll and photosynthetic reaction centers resembling those of purple photosynthetic bacteria when grown aerobically ex planta under a light-dark cycle. bacteriochlorophyll formation was not observed under continuous dark or light growth conditions. the amount of pigment formed was similar to that previously found in aerobic photosynthetic bacteria. stem nodules appear to fix nitrogen pho ... | 1990 | 16348349 |
| phylogenetic analysis of bradyrhizobium japonicum and photosynthetic stem-nodulating bacteria from aeschynomene species grown in separated geographical regions. | nearly complete and short partial 16s rrna sequences were derived from pcr-amplified ribosomal dnas of bradyrhizobium japonicum usda 136 and usda 110 and five strains of bacteriochlorophyll-synthesizing bacteria isolated from stem nodules of aeschynomene indica and other aeschynomene species growing in different geographic regions, including india, the philippines and north america. we confirmed that the five stem-nodulating strains examined synthesize bacteriochlorophyll a, and the absorption s ... | 1994 | 16349221 |
| nonpigmented and bacteriochlorophyll-containing bradyrhizobia isolated from aeschynomene indica. | the legume genus aeschynomene is unusual, since many species develop stem nodules and the bradyrhizobia isolated from these nodules produce bacteriochlorophyll (bchl). evidence is presented that the bradyrhizobia of aeschynomene indica have wide distribution throughout the world, since a. indica was nodulated when grown in 58 soils collected in 14 different countries. only 38 of 79 isolates tested synthesized bchl and carotenoids during heterotrophic growth. nine isolates produced bchl constitut ... | 1995 | 16534933 |
| proposal for combining bradyrhizobium spp. (aeschynomene indica) with blastobacter denitrificans and to transfer blastobacter denitrificans (hirsch and muller, 1985) to the genus bradyrhizobium as bradyrhizobium denitrificans (comb. nov.). | the symbiotic bradyrhizobia of aeschynomene indica and the aquatic budding bacterium blastobacter denitrificans have much in common and this study broadens the characters that are shared between the two. the 23s rrna gene sequences of the bradyrhizobial isolates were most similar to each other and to the sequence of bl. denitrificans. evidence for the presence of photosynthetic genes in the genome of bl. denitrificans was obtained by pcr using primers to the conserved m subunit (pufm) of the pho ... | 2006 | 16564957 |
| large-scale transposon mutagenesis of photosynthetic bradyrhizobium sp. strain ors278 reveals new genetic loci putatively important for nod-independent symbiosis with aeschynomene indica. | photosynthetic bradyrhizobium strains possess the unusual ability to form nitrogen-fixing nodules on a specific group of legumes in the absence of nod factors. to obtain insight into the bacterial genes involved in this nod-independent symbiosis, we screened 15,648 tn5 mutants of bradyrhizobium sp. strain ors278 for clones affected in root symbiosis with aeschynomene indica. from the 268 isolated mutants, 120 mutants were altered in nodule development (ndv(-)) and 148 mutants were found to be de ... | 2010 | 20459315 |
| the nod factor-independent symbiotic signaling pathway: development of agrobacterium rhizogenes-mediated transformation for the legume aeschynomene indica. | the nitrogen-fixing symbiosis between aeschynomene indica and photosynthetic bradyrhizobia is the only legume-rhizobium association described to date that does not require lipochito-oligosaccharide nod factors (nf). to assist in deciphering the molecular basis of this nf-independent interaction, we have developed a protocol for agrobacterium rhizogenes-mediated transformation of a. indica. the cotransformation frequency (79%), the nodulation efficiency of transgenic roots (90%), and the expressi ... | 2010 | 21039272 |
| bacterial rubisco is required for efficient bradyrhizobium/aeschynomene symbiosis. | rhizobia and legume plants establish symbiotic associations resulting in the formation of organs specialized in nitrogen fixation. in such organs, termed nodules, bacteria differentiate into bacteroids which convert atmospheric nitrogen and supply the plant with organic nitrogen. as a counterpart, bacteroids receive carbon substrates from the plant. this rather simple model of metabolite exchange underlies symbiosis but does not describe the complexity of bacteroids' central metabolism. a previo ... | 2011 | 21750740 |
| diversity analyses of aeschynomene symbionts in tropical africa and central america reveal that nod-independent stem nodulation is not restricted to photosynthetic bradyrhizobia. | tropical aquatic legumes of the genus aeschynomene are unique in that they can be stem-nodulated by photosynthetic bradyrhizobia. moreover, a recent study demonstrated that two aeschynomene indica symbionts lack canonical nod genes, thereby raising questions about the distribution of such atypical symbioses among rhizobial-legume interactions. population structure and genomic diversity were compared among stem-nodulating bradyrhizobia isolated from various aeschynomene species of central america ... | 2010 | 21966910 |
| a unique bicyclic monosaccharide from the bradyrhizobium lipopolysaccharide and its role in the molecular interaction with plants. | sugar coat: the nitrogen-fixing soil bacterium bradyrhizobium sp. btai1 is coated with a unique lipopolysaccharide that does not induce innate immune responses in its host plant aeschynomene indica or in different plant families. the chemical nature of the monosaccharide forming the polymer is unprecedented in nature, which helps to avoid "harmful" recognition by its symbiotic host. | 2011 | 22058060 |
| a proteomic approach of bradyrhizobium/aeschynomene root and stem symbioses reveals the importance of the fixa locus for symbiosis. | rhizobia are soil bacteria that are able to form symbiosis with plant hosts of the legume family. these associations result in the formation of organs, called nodules in which bacteria fix atmospheric nitrogen to the benefit of the plant. most of our knowledge on the metabolism and the physiology of the bacteria during symbiosis derives from studying roots nodules of terrestrial plants. here we used a proteomics approach to investigate the bacterial physiology of photosynthetic bradyrhizobium sp ... | 2014 | 24590127 |
| genome analysis suggests that the soil oligotrophic bacterium agromonas oligotrophica (bradyrhizobium oligotrophicum) is a nitrogen-fixing symbiont of aeschynomene indica. | agromonas oligotrophica (bradyrhizobium oligotrophicum) s58(t) is a nitrogen-fixing oligotrophic bacterium isolated from paddy field soil that is able to grow in extra-low-nutrient environments. here, the complete genome sequence of s58 was determined. the s58 genome was found to comprise a circular chromosome of 8,264,165 bp with an average gc content of 65.1% lacking nodabc genes and the typical symbiosis island. the genome showed a high level of similarity to the genomes of bradyrhizobium sp. ... | 2013 | 23396330 |
| preferential association of endophytic bradyrhizobia with different rice cultivars and its implications for rice endophyte evolution. | plant colonization by bradyrhizobia is found not only in leguminous plants but also in nonleguminous species such as rice. to understand the evolution of the endophytic symbiosis of bradyrhizobia, the effect of the ecosystems of rice plantations on their associations was investigated. samples were collected from various rice (oryza sativa) tissues and crop rotational systems. the rice endophytic bradyrhizobia were isolated on the basis of oligotrophic properties, selective medium, and nodulation ... | 2015 | 25710371 |
| bradyrhizobium guangdongense sp. nov. and bradyrhizobium guangxiense sp. nov., isolated from effective nodules of peanut. | seven slow-growing rhizobia isolated from effective nodules of arachis hypogaea were assigned to the genus bradyrhizobium based on sharing 96.3-99.9 % 16s rrna gene sequence similarity with the type strains of recognized bradyrhizobium species. multilocus sequence analysis of glnii, reca, gyrb and dnak genes indicated that the seven strains belonged to two novel species represented by ccbau 51649t and ccbau 53363t. strain ccbau 51649t shared 94, 93.4, 92.3 and 94.9 % and ccbau 53363t shared 91.4 ... | 2015 | 26409482 |
| phytoremediation of soil co-contaminated with heavy metals and tnt using four plant species. | we investigated the germination, growth rates and uptake of contaminants of four plant species, barnyard grass (echinochloa crusgalli), sunflower (helianthus annuus), indian mallow (abutilon avicennae) and indian jointvetch (aeschynomene indica), grown in soil contaminated with cadmium (cd), lead (pb) and 2,4,6-trinitrotoluene (tnt). these contaminants are typically found at shooting ranges. experiments were carried out over 180 days using both single plant cultures and cultures containing an eq ... | 2007 | 17990167 |
| synthesis of bradyrhizose, a unique inositol-fused monosaccharide relevant to a nod-factor independent nitrogen fixation. | the symbiosis of bradyrhizobium sp. btai1 with its host plant aeschynomene indica relies on a nod-factor independent mechanism, wherein the bradyrhizobium o-antigen is regarded as a key factor. this o-antigen polysaccharide is composed of a unique c10 monosaccharide, namely bradyrhizose, which has a galactose-inositol trans-fused scaffold, via a homogeneous α-(1 → 7)-linkage. herein, we report the first synthesis of bradyrhizose. the scalable synthesis requires 26 steps in a high overall yield o ... | 2015 | 25797311 |
| [development characteristics of aquatic plants in a constructed wetland for treating urban drinking water source at its initial operation stage]. | the development characteristics and improvement measures of aquatic plants were studied in shijiuyang constructed wetland (scw) at its initial operation stage. scw was a large-scale wetland aiming to help relieve the source water pollution in jiaxing city. a checklist of vascular plants in scw was built, and species composition, life forms, biomass and association distributions were examined. our objectives were to examine the diversity and community structure of aquatic plants in scw at its ini ... | 2011 | 22619945 |
| degradation of phenanthrene and pyrene in rhizosphere of grasses and legumes. | phytoremediation is an emerging technology for the remediation of organic soil pollutants such as phenanthrene and pyrene (polycyclic aromatic hydrocarbons, pahs). the pah degradation ability of four native korean plant species (panicum bisulcatum, echinogalus crus-galli, astragalus membranaceus, and aeschynomene indica) was compared in the greenhouse. during the 80-day experiment, soil samples were collected and analyzed periodically to determine the residual pah content and microbial activity. ... | 2008 | 17959304 |