| an mlsa approach for the taxonomic update of the splendidus clade, a lineage containing several fish and shellfish pathogenic vibrio spp. | a multilocus sequence analysis was undertaken in order to redefine the splendidus clade of the genus vibrio, a large group of species containing several pathogenic members that affect fish and shellfish, and are difficult to identify through both phenotypic and genotypic approaches. the study included analysis of partial sequences of reca, gyrb, mreb, rpod and pyrh genes, as well as the 16s rrna gene. seventeen type strain species were included that were complemented with other reference strains ... | 2016 | 27238556 |
| inhibition of marine vibrio sp. by pyoverdine from pseudomonas aeruginosa pa1. | siderophores are low-molecular-weight chemicals that are secreted by many microorganisms to chelate iron from the external environment in order to facilitate their growth and diverse metabolisms. in this study, a fluorescent siderophore, pyoverdine, secreted by pseudomonas aeruginosa pa1 was purified by affinity chromatography using cu-sepharose. pyoverdine was determined to have a molecular mass of 1333.54 da, as determined by maldi-tof/tof, and belong to type i pyoverdine, as determined by pcr ... | 2016 | 26476308 |
| genomic mapping of phosphorothioates reveals partial modification of short consensus sequences. | bacterial phosphorothioate (pt) dna modifications are incorporated by dnd proteins a-e and often function with dndf-h as a restriction-modification (r-m) system, as in escherichia coli b7a. however, bacteria such as vibrio cyclitrophicus ff75 lack dndf-h, which points to other pt functions. here we report two novel, orthogonal technologies to map pts across the genomes of b7a and ff75 with >90% agreement: single molecule, real-time sequencing and deep sequencing of iodine-induced cleavage at pt ... | 2014 | 24899568 |
| co-inoculation of capitella sp. i with its synergistic bacteria enhances degradation of organic matter in organically enriched sediment below fish farms. | a polychaete, capitella sp. i has been shown to degrade organics actively in organically enriched sediment below fish farms. our aim of the present study is to enhance the biological treatment of sediment by co-inoculation of capitella sp. i with bacterial isolates that possess high degrading potential for organic matter. we isolated a total of 200 bacterial strains from fecal pellets, burrow lining, worm body, and sediment, and selected six of them for the degradation experiments in the sedimen ... | 2008 | 18037450 |
| vibrio cyclotrophicus sp. nov., a polycyclic aromatic hydrocarbon (pah)-degrading marine bacterium. | strain p-2p44t was isolated from creosote-contaminated marine sediments by using a most-probable number procedure in which phenanthrene was the sole carbon and energy source. growth experiments showed that p-2p44t utilized several two- and three-ring polycyclic aromatic hydrocarbons (pahs) as substrates, including naphthalene, 2-methylnaphthalene and phenanthrene. additionally, gas-chromatography experiments showed that p-2p44t degraded several other pahs, though it was unable to use them as sol ... | 2001 | 11211274 |
| unambiguous identification and discovery of bacterial siderophores by direct injection 21 tesla fourier transform ion cyclotron resonance mass spectrometry. | under iron-limiting conditions, bacteria produce low molecular mass fe(iii) binding molecules known as siderophores to sequester the fe(iii), along with other elements, increasing their bioavailability. siderophores are thought to influence iron cycling and biogeochemistry in both marine and terrestrial ecosystems and hence the need for rapid, confident characterization of these compounds has increased. in this study, the type of siderophores produced by two marine bacterial species, synechococc ... | 2017 | 27905613 |
| phylogenomic network and comparative genomics reveal a diverged member of the φkz-related group, marine vibrio phage φjm-2012. | bacteriophages are the largest reservoir of genetic diversity. here we describe the novel phage φjm-2012. this natural isolate from marine vibrio cyclitrophicus possesses very few gene contents relevant to other well-studied marine vibrio phages. to better understand its evolutionary history, we built a mathematical model of pairwise relationships among 1,221 phage genomes, in which the genomes (nodes) are linked by edges representing the normalized number of shared orthologous protein families. ... | 2013 | 24067958 |
| polyunsaturated fatty acid production by marine bacteria. | polyunsaturated fatty acids are important in maintaining human health. limitations associated with current sources of ω-3 fatty acids and ω-6 fatty acids, from animal and plant sources, have led to increased interest in microbial production. marine bacteria may provide a suitable alternative, although the isolation of production strains and the identification of operating conditions must be addressed before manufacturing processes become economically viable. marine isolate 560 was identified as ... | 2013 | 23525832 |